Rorug03G0331000
ERF Family

Polyketide cyclase dehydrase and lipid transport superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001074
Physical Location & Seq
Reverse (-)
38418829 .. 38421895
3067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0331000.1

Sequence Viewer

Length: 660 bp
ATGGCTCAATTGAGCGACTTCAGCCTGCGATTTCTTCCCGTGAGCACTTCGATTGAAACTGGCAAAGTCGGAATTGACATATCTAGAAGCTCGATTGATCTGAAATGGGTCTTCTCAGCATTATTCGAAAAATTAAAAGGAAGGAGAAAGAAATACGTATACTCATGCGGGCTTGACAATTCGGGAAAAACTACAATTGTGCTGAGGATTAATGGGGAGGACACCAGCGTCGTCAGTCCCACCCTCGGCTTCAACATCAAGACTCTCACTTACCATAAGTATACACTTAACATATGGGATGTGGGGGGTCAAAAAACAATACGATCTTATTGGAGAAACTATTTTGAACAAACTGATGGTCTCGTATGGGTAGTTGACAGTTCTGATCTTAGAAGATTAGATGACTGCAAAATGGAGCTTGATAATCTTTTGAAGGAAGAGAGGCTATCAGGATCGTCCTTACTGATACTAGCAAATAAGCAGGACATAAAAGGTGCCCTTACTCCACAAGAAATTGCCAAGGTTTTGAACTTGGAGGCTATGGATAAAACTCGACACTGGAATATTGTTGGGTGCAGTGCATACACGGGTGAGGGGCTGCTTCAGGGATTTGATTGGTTGGTCCAAGACATTGCCTCTCGGATCTATGTGCTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

219

Amino Acids

24.83

Weight (kDa)

7.65

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 39 - 211 1.3e-62 ADP-ribosylation factor family
SRPRB PF09439 51 - 179 4.9e-07 Signal recognition particle receptor beta subunit
Roc PF08477 56 - 162 4.7e-10 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 57 - 211 3.1e-10 Ras family
G-alpha PF00503 80 - 135 1.5e-06 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
AccB1I GGYRCC 1 cut(s) 494
AccI GTMKAC 2 cut(s) 159, 281
AciI CCGC 1 cut(s) 168
AclWI GGATC 2 cut(s) 460, 650
AcuI CTGAAG 2 cut(s) 4, 587
AfiI CCNNNNNNNGG 1 cut(s) 245
AgsI TTSAA 5 cut(s) 56, 253, 347, 433, 529
AluBI AGCT 2 cut(s) 90, 418
AluI AGCT 2 cut(s) 90, 418
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 2 cut(s) 460, 650
ApeKI GCWGC 1 cut(s) 598
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
AseI ATTAAT 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 622
AsuHPI GGTGA 1 cut(s) 602
AsuII TTCGAA 1 cut(s) 126
AvaII GGWCC 1 cut(s) 622
BaeGI GKGCMC 1 cut(s) 499
BanI GGYRCC 1 cut(s) 494
BbsI GAAGAC 1 cut(s) 103
Bbv12I GWGCWC 1 cut(s) 47
BbvCI CCTCAGC 1 cut(s) 203
BbvI GCAGC 1 cut(s) 585
BccI CCATC 1 cut(s) 350
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 2 cut(s) 84, 470
BisI GCNGC 1 cut(s) 599
BlsI GCNGC 1 cut(s) 600
Bme18I GGWCC 1 cut(s) 622
BmgT120I GGNCC 1 cut(s) 622
BmiI GGNNCC 1 cut(s) 496
BpiI GAAGAC 1 cut(s) 103
Bpu10I CCTNAGC 1 cut(s) 203
Bpu14I TTCGAA 1 cut(s) 126
BsaAI YACGTR 1 cut(s) 157
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 2 cut(s) 244, 519
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse1I ACTGG 2 cut(s) 64, 563
Bse3DI GCAATG 1 cut(s) 630
BseDI CCNNGG 2 cut(s) 244, 519
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 2 cut(s) 129, 194
BseNI ACTGG 2 cut(s) 64, 563
BseSI GKGCMC 1 cut(s) 499
BseXI GCAGC 1 cut(s) 585
BsgI GTGCAG 1 cut(s) 595
BshNI GGYRCC 1 cut(s) 494
BsiHKAI GWGCWC 1 cut(s) 47
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 1 cut(s) 365
BsmFI GGGAC 1 cut(s) 222
Bso31I GGTCTC 1 cut(s) 365
Bsp119I TTCGAA 1 cut(s) 126
Bsp1286I GDGCHC 2 cut(s) 47, 499
Bsp143I GATC 5 cut(s) 97, 323, 385, 452, 642
BspACI CCGC 1 cut(s) 168
BspCNI CTCAG 2 cut(s) 128, 195
BspLI GGNNCC 1 cut(s) 496
BspPI GGATC 2 cut(s) 460, 650
BspT104I TTCGAA 1 cut(s) 126
BspT107I GGYRCC 1 cut(s) 494
BspTNI GGTCTC 1 cut(s) 365
BsrDI GCAATG 1 cut(s) 630
BsrI ACTGG 2 cut(s) 64, 563
BssECI CCNNGG 2 cut(s) 244, 519
BssMI GATC 5 cut(s) 97, 323, 385, 452, 642
BssNAI GTATAC 2 cut(s) 160, 282
BssT1I CCWWGG 1 cut(s) 519
Bst1107I GTATAC 2 cut(s) 160, 282
Bst4CI ACNGT 1 cut(s) 380
Bst6I CTCTTC 1 cut(s) 432
BstBAI YACGTR 1 cut(s) 157
BstBI TTCGAA 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 26, 170
BstDEI CTNAG 3 cut(s) 115, 203, 389
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 5 cut(s) 100, 326, 388, 455, 645
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 5 cut(s) 97, 323, 385, 452, 642
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstSLI GKGCMC 1 cut(s) 499
BstSNI TACGTA 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 585
BstV2I GAAGAC 1 cut(s) 103
BstX2I RGATCY 1 cut(s) 642
BstYI RGATCY 1 cut(s) 642
BstZ17I GTATAC 2 cut(s) 160, 282
BtsCI GGATG 1 cut(s) 304
BtsI GCAGTG 1 cut(s) 583
BtsIMutI CAGTG 2 cut(s) 556, 583
Cac8I GCNNGC 2 cut(s) 26, 170
Cfr13I GGNCC 1 cut(s) 622
CseI GACGC 1 cut(s) 217
CviAII CATG 1 cut(s) 165
CviJI RGCY 9 cut(s) 5, 24, 90, 172, 249, 418, 445, 539, 598
CviKI_1 RGCY 9 cut(s) 5, 24, 90, 172, 249, 418, 445, 539, 598
DdeI CTNAG 3 cut(s) 115, 203, 389
DpnI GATC 5 cut(s) 99, 325, 387, 454, 644
DpnII GATC 5 cut(s) 97, 323, 385, 452, 642
DrdI GACNNNNNNGTC 1 cut(s) 227
DseDI GACNNNNNNGTC 1 cut(s) 227
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
Eco105I TACGTA 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 519
Eco31I GGTCTC 1 cut(s) 365
Eco47I GGWCC 1 cut(s) 622
Eco57I CTGAAG 2 cut(s) 4, 587
EcoT14I CCWWGG 1 cut(s) 519
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 1 cut(s) 168
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FaqI GGGAC 1 cut(s) 222
FatI CATG 1 cut(s) 164
FauI CCCGC 1 cut(s) 161
FauNDI CATATG 1 cut(s) 293
FblI GTMKAC 2 cut(s) 159, 281
Fnu4HI GCNGC 1 cut(s) 599
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 1 cut(s) 599
FspBI CTAG 2 cut(s) 84, 470
GluI GCNGC 1 cut(s) 599
HgaI GACGC 1 cut(s) 217
Hin1II CATG 1 cut(s) 168
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HinfI GANTC 1 cut(s) 262
HphI GGTGA 1 cut(s) 602
Hpy166II GTNNAC 3 cut(s) 160, 282, 376
Hpy188I TCNGA 4 cut(s) 71, 102, 385, 642
Hpy188III TCNNGA 4 cut(s) 84, 183, 259, 450
Hpy8I GTNNAC 3 cut(s) 160, 282, 376
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 2 cut(s) 135, 427
HpyCH4III ACNGT 1 cut(s) 380
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 3 cut(s) 408, 576, 581
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
HpyF3I CTNAG 3 cut(s) 115, 203, 389
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 1 cut(s) 168
Kzo9I GATC 5 cut(s) 97, 323, 385, 452, 642
LmnI GCTCC 1 cut(s) 415
LpnPI CCDG 7 cut(s) 38, 45, 238, 435, 467, 544, 590
Lsp1109I GCAGC 1 cut(s) 585
MaeI CTAG 2 cut(s) 84, 470
MaeII ACGT 1 cut(s) 156
MalI GATC 5 cut(s) 99, 325, 387, 454, 644
MboI GATC 5 cut(s) 97, 323, 385, 452, 642
MboII GAAGA 4 cut(s) 26, 103, 405, 449
MfeI CAATTG 2 cut(s) 8, 195
MflI RGATCY 1 cut(s) 642
MhlI GDGCHC 2 cut(s) 47, 499
MluCI AATT 6 cut(s) 8, 72, 131, 178, 195, 513
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 7 cut(s) 198, 211, 254, 435, 529, 586, 646
MseI TTAA 3 cut(s) 134, 210, 288
MunI CAATTG 2 cut(s) 8, 195
MwoI GCNNNNNNNGC 1 cut(s) 21
NdeI CATATG 1 cut(s) 293
NdeII GATC 5 cut(s) 97, 323, 385, 452, 642
NlaIII CATG 1 cut(s) 168
NlaIV GGNNCC 1 cut(s) 496
NmeAIII GCCGAG 1 cut(s) 225
NspV TTCGAA 1 cut(s) 126
PkrI GCNGC 1 cut(s) 600
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
Ppu21I YACGTR 1 cut(s) 157
PshBI ATTAAT 1 cut(s) 210
PspN4I GGNNCC 1 cut(s) 496
PspPI GGNCC 1 cut(s) 622
PsuI RGATCY 1 cut(s) 642
SaqAI TTAA 3 cut(s) 134, 210, 288
SatI GCNGC 1 cut(s) 599
Sau3AI GATC 5 cut(s) 97, 323, 385, 452, 642
Sau96I GGNCC 1 cut(s) 622
SchI GAGTC 1 cut(s) 256
SduI GDGCHC 2 cut(s) 47, 499
SetI ASST 5 cut(s) 92, 159, 420, 496, 525
SfuI TTCGAA 1 cut(s) 126
SinI GGWCC 1 cut(s) 622
SnaBI TACGTA 1 cut(s) 157
Sse9I AATT 6 cut(s) 8, 72, 131, 178, 195, 513
SsiI CCGC 1 cut(s) 168
SspI AATATT 1 cut(s) 565
SspMI CTAG 2 cut(s) 84, 470
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 1 cut(s) 380
TaiI ACGT 1 cut(s) 159
TaqI TCGA 4 cut(s) 50, 92, 126, 553
TasI AATT 6 cut(s) 8, 72, 131, 178, 195, 513
Tru1I TTAA 3 cut(s) 134, 210, 288
Tru9I TTAA 3 cut(s) 134, 210, 288
TscAI CASTG 2 cut(s) 563, 583
TseI GCWGC 1 cut(s) 598
TspRI CASTG 2 cut(s) 563, 583
VpaK11BI GGWCC 1 cut(s) 622
VspI ATTAAT 1 cut(s) 210
XbaI TCTAGA 1 cut(s) 83
XmiI GTMKAC 2 cut(s) 159, 281
XspI CTAG 2 cut(s) 84, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.