Zinc knuckle (CCHC-type) family protein
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Plus-3 | PF03126 | 912 - 1012 | 4.2e-13 | Plus-3 domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 89 |
| Acc36I | ACCTGC | 2 cut(s) | 89, 101 |
| AccI | GTMKAC | 1 cut(s) | 170 |
| AciI | CCGC | 4 cut(s) | 465, 2018, 2827, 2856 |
| AclWI | GGATC | 5 cut(s) | 620, 633, 753, 865, 2532 |
| AcuI | CTGAAG | 3 cut(s) | 1815, 2145, 2270 |
| AcvI | CACGTG | 1 cut(s) | 1352 |
| AcyI | GRCGYC | 1 cut(s) | 351 |
| AfaI | GTAC | 3 cut(s) | 807, 1335, 2712 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 512, 874, 1648, 2114, 2589 |
| AflII | CTTAAG | 1 cut(s) | 2165 |
| AgeI | ACCGGT | 1 cut(s) | 2665 |
| AjnI | CCWGG | 4 cut(s) | 538, 870, 1222, 1464 |
| AjuI | GAANNNNNNNTTGG | 3 cut(s) | 2984, 3016, 3089 |
| AleI | CACNNNNGTG | 1 cut(s) | 2008 |
| Alw21I | GWGCWC | 1 cut(s) | 46 |
| Alw26I | GTCTC | 6 cut(s) | 380, 1824, 1993, 2540, 2590, 2757 |
| AlwI | GGATC | 5 cut(s) | 620, 633, 753, 865, 2532 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 35, 814 |
| Ama87I | CYCGRG | 1 cut(s) | 2536 |
| AoxI | GGCC | 4 cut(s) | 138, 230, 767, 2976 |
| ApeKI | GCWGC | 7 cut(s) | 305, 839, 1585, 1916, 1963, 2015, 2201 |
| AseI | ATTAAT | 1 cut(s) | 1889 |
| AsiGI | ACCGGT | 1 cut(s) | 2665 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 396, 522, 1289 |
| AspS9I | GGNCC | 5 cut(s) | 139, 182, 467, 768, 1462 |
| AsuC2I | CCSGG | 1 cut(s) | 143 |
| AsuHPI | GGTGA | 5 cut(s) | 195, 554, 647, 2237, 2507 |
| AvaI | CYCGRG | 1 cut(s) | 2536 |
| AvaII | GGWCC | 3 cut(s) | 182, 467, 1462 |
| AxyI | CCTNAGG | 2 cut(s) | 1512, 1647 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2694, 2727 |
| BamHI | GGATCC | 1 cut(s) | 625 |
| BanII | GRGCYC | 1 cut(s) | 150 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2727, 2759 |
| BbrPI | CACGTG | 1 cut(s) | 1352 |
| BbsI | GAAGAC | 3 cut(s) | 645, 2328, 3078 |
| Bbv12I | GWGCWC | 1 cut(s) | 46 |
| BbvCI | CCTCAGC | 1 cut(s) | 3017 |
| BbvI | GCAGC | 7 cut(s) | 292, 851, 1572, 1928, 1975, 2027, 2188 |
| BccI | CCATC | 5 cut(s) | 650, 1010, 2188, 2770, 3070 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 351, 385 |
| BciT130I | CCWGG | 4 cut(s) | 540, 872, 1224, 1466 |
| BciVI | GTATCC | 1 cut(s) | 2998 |
| BclI | TGATCA | 1 cut(s) | 1342 |
| BcnI | CCSGG | 1 cut(s) | 143 |
| BcoDI | GTCTC | 6 cut(s) | 380, 1824, 1993, 2540, 2590, 2757 |
| BfaI | CTAG | 6 cut(s) | 707, 1131, 1568, 2148, 2852, 2862 |
| BfmI | CTRYAG | 2 cut(s) | 171, 2440 |
| BfrI | CTTAAG | 1 cut(s) | 2165 |
| BfuAI | ACCTGC | 2 cut(s) | 89, 101 |
| BfuI | GTATCC | 1 cut(s) | 2998 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2184 |
| BglII | AGATCT | 2 cut(s) | 1021, 2551 |
| BisI | GCNGC | 8 cut(s) | 306, 840, 1586, 1917, 1964, 2016, 2202, 2828 |
| BlsI | GCNGC | 8 cut(s) | 307, 841, 1587, 1918, 1965, 2017, 2203, 2829 |
| Bme1390I | CCNGG | 5 cut(s) | 143, 540, 872, 1224, 1466 |
| Bme18I | GGWCC | 3 cut(s) | 182, 467, 1462 |
| BmeT110I | CYCGRG | 1 cut(s) | 2536 |
| BmgT120I | GGNCC | 5 cut(s) | 139, 182, 467, 768, 1462 |
| BmiI | GGNNCC | 4 cut(s) | 141, 147, 603, 627 |
| BmrFI | CCNGG | 5 cut(s) | 143, 540, 872, 1224, 1466 |
| BmsI | GCATC | 7 cut(s) | 307, 334, 492, 561, 1048, 1387, 2149 |
| BpiI | GAAGAC | 3 cut(s) | 645, 2328, 3078 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2494, 2526 |
| BpmI | CTGGAG | 3 cut(s) | 534, 803, 2825 |
| Bpu10I | CCTNAGC | 1 cut(s) | 3017 |
| BpuEI | CTTGAG | 4 cut(s) | 1541, 2496, 3026, 3097 |
| BpuMI | CCSGG | 1 cut(s) | 143 |
| BsaAI | YACGTR | 1 cut(s) | 1352 |
| BsaBI | GATNNNNATC | 2 cut(s) | 660, 1020 |
| BsaHI | GRCGYC | 1 cut(s) | 351 |
| BsaI | GGTCTC | 1 cut(s) | 1824 |
| BsaJI | CCNNGG | 6 cut(s) | 141, 757, 771, 871, 1451, 1465 |
| BsaWI | WCCGGW | 2 cut(s) | 1991, 2665 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 137, 167 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 512, 874, 1648, 2114, 2589 |
| Bse118I | RCCGGY | 3 cut(s) | 205, 1670, 2665 |
| Bse1I | ACTGG | 8 cut(s) | 29, 517, 786, 1423, 1967, 2497, 2699, 2890 |
| Bse21I | CCTNAGG | 2 cut(s) | 1512, 1647 |
| Bse3DI | GCAATG | 3 cut(s) | 196, 533, 2136 |
| Bse8I | GATNNNNATC | 2 cut(s) | 660, 1020 |
| BseBI | CCWGG | 4 cut(s) | 540, 872, 1224, 1466 |
| BseDI | CCNNGG | 6 cut(s) | 141, 757, 771, 871, 1451, 1465 |
| BseJI | GATNNNNATC | 2 cut(s) | 660, 1020 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 512, 874, 1648, 2114, 2589 |
| BseMI | GCAATG | 3 cut(s) | 196, 533, 2136 |
| BseMII | CTCAG | 8 cut(s) | 231, 288, 489, 806, 1503, 1638, 2412, 3008 |
| BseNI | ACTGG | 8 cut(s) | 29, 517, 786, 1423, 1967, 2497, 2699, 2890 |
| BseRI | GAGGAG | 2 cut(s) | 929, 1973 |
| BseXI | GCAGC | 7 cut(s) | 292, 851, 1572, 1928, 1975, 2027, 2188 |
| BsgI | GTGCAG | 4 cut(s) | 117, 858, 1935, 2034 |
| BshFI | GGCC | 4 cut(s) | 140, 232, 769, 2978 |
| BshTI | ACCGGT | 1 cut(s) | 2665 |
| BsiHKAI | GWGCWC | 1 cut(s) | 46 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2536 |
| BsiSI | CCGG | 5 cut(s) | 143, 206, 1671, 1992, 2666 |
| BslFI | GGGAC | 2 cut(s) | 1201, 2418 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 512, 874, 1648, 2114, 2589 |
| BsmAI | GTCTC | 6 cut(s) | 380, 1824, 1993, 2540, 2590, 2757 |
| BsmFI | GGGAC | 2 cut(s) | 1201, 2418 |
| BsmI | GAATGC | 3 cut(s) | 869, 2366, 2929 |
| BsnI | GGCC | 4 cut(s) | 140, 232, 769, 2978 |
| Bso31I | GGTCTC | 1 cut(s) | 1824 |
| BsoBI | CYCGRG | 1 cut(s) | 2536 |
| Bsp1286I | GDGCHC | 2 cut(s) | 46, 150 |
| Bsp1407I | TGTACA | 2 cut(s) | 805, 1333 |
| BspACI | CCGC | 4 cut(s) | 465, 2018, 2827, 2856 |
| BspANI | GGCC | 4 cut(s) | 140, 232, 769, 2978 |
| BspCNI | CTCAG | 8 cut(s) | 232, 287, 490, 807, 1504, 1639, 2411, 3009 |
| BspHI | TCATGA | 2 cut(s) | 1492, 2214 |
| BspLI | GGNNCC | 4 cut(s) | 141, 147, 603, 627 |
| BspMAI | CTGCAG | 1 cut(s) | 2444 |
| BspMI | ACCTGC | 2 cut(s) | 89, 101 |
| BspPI | GGATC | 5 cut(s) | 620, 633, 753, 865, 2532 |
| BspTI | CTTAAG | 1 cut(s) | 2165 |
| BspTNI | GGTCTC | 1 cut(s) | 1824 |
| BsrDI | GCAATG | 3 cut(s) | 196, 533, 2136 |
| BsrFI | RCCGGY | 3 cut(s) | 205, 1670, 2665 |
| BsrGI | TGTACA | 2 cut(s) | 805, 1333 |
| BsrI | ACTGG | 8 cut(s) | 29, 517, 786, 1423, 1967, 2497, 2699, 2890 |
| BssAI | RCCGGY | 3 cut(s) | 205, 1670, 2665 |
| BssECI | CCNNGG | 6 cut(s) | 141, 757, 771, 871, 1451, 1465 |
| BssNI | GRCGYC | 1 cut(s) | 351 |
| BssT1I | CCWWGG | 3 cut(s) | 757, 771, 1451 |
| Bst2UI | CCWGG | 4 cut(s) | 540, 872, 1224, 1466 |
| Bst6I | CTCTTC | 5 cut(s) | 252, 290, 1148, 1822, 2493 |
| BstACI | GRCGYC | 1 cut(s) | 351 |
| BstAFI | CTTAAG | 1 cut(s) | 2165 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1667 |
| BstAUI | TGTACA | 2 cut(s) | 805, 1333 |
| BstBAI | YACGTR | 1 cut(s) | 1352 |
| BstC8I | GCNNGC | 6 cut(s) | 77, 310, 349, 360, 1527, 2029 |
| BstEII | GGTNACC | 1 cut(s) | 2243 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2587 |
| BstMAI | GTCTC | 6 cut(s) | 380, 1824, 1993, 2540, 2590, 2757 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 836, 1667, 2024, 2184, 2754, 2827 |
| BstNI | CCWGG | 4 cut(s) | 540, 872, 1224, 1466 |
| BstNSI | RCATGY | 3 cut(s) | 650, 1775, 2385 |
| BstPI | GGTNACC | 1 cut(s) | 2243 |
| BstSCI | CCNGG | 5 cut(s) | 141, 538, 870, 1222, 1464 |
| BstSFI | CTRYAG | 2 cut(s) | 171, 2440 |
| BstV1I | GCAGC | 7 cut(s) | 292, 851, 1572, 1928, 1975, 2027, 2188 |
| BstV2I | GAAGAC | 3 cut(s) | 645, 2328, 3078 |
| BstX2I | RGATCY | 3 cut(s) | 625, 1021, 2551 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1400 |
| BstYI | RGATCY | 3 cut(s) | 625, 1021, 2551 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1512, 1647 |
| BsuI | GTATCC | 1 cut(s) | 2998 |
| BsuRI | GGCC | 4 cut(s) | 140, 232, 769, 2978 |
| BtsIMutI | CAGTG | 9 cut(s) | 36, 68, 91, 123, 1132, 1912, 1951, 2406, 2632 |
| BveI | ACCTGC | 2 cut(s) | 89, 101 |
| Cac8I | GCNNGC | 6 cut(s) | 77, 310, 349, 360, 1527, 2029 |
| CaiI | CAGNNNCTG | 2 cut(s) | 35, 814 |
| CciI | TCATGA | 2 cut(s) | 1492, 2214 |
| Cfr10I | RCCGGY | 3 cut(s) | 205, 1670, 2665 |
| Cfr13I | GGNCC | 5 cut(s) | 139, 182, 467, 768, 1462 |
| CseI | GACGC | 2 cut(s) | 340, 2810 |
| CsiI | ACCWGGT | 1 cut(s) | 1222 |
| Csp6I | GTAC | 3 cut(s) | 806, 1334, 2711 |
| CspAI | ACCGGT | 1 cut(s) | 2665 |
| CviQI | GTAC | 3 cut(s) | 806, 1334, 2711 |
| Eam1104I | CTCTTC | 5 cut(s) | 252, 290, 1148, 1822, 2493 |
| EarI | CTCTTC | 5 cut(s) | 252, 290, 1148, 1822, 2493 |
| EciI | GGCGGA | 1 cut(s) | 2871 |
| Eco130I | CCWWGG | 3 cut(s) | 757, 771, 1451 |
| Eco147I | AGGCCT | 1 cut(s) | 232 |
| Eco24I | GRGCYC | 1 cut(s) | 150 |
| Eco31I | GGTCTC | 1 cut(s) | 1824 |
| Eco32I | GATATC | 1 cut(s) | 1272 |
| Eco47I | GGWCC | 3 cut(s) | 182, 467, 1462 |
| Eco57I | CTGAAG | 3 cut(s) | 1815, 2145, 2270 |
| Eco72I | CACGTG | 1 cut(s) | 1352 |
| Eco81I | CCTNAGG | 2 cut(s) | 1512, 1647 |
| Eco88I | CYCGRG | 1 cut(s) | 2536 |
| Eco91I | GGTNACC | 1 cut(s) | 2243 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2587 |
| EcoO109I | RGGNCCY | 1 cut(s) | 768 |
| EcoO65I | GGTNACC | 1 cut(s) | 2243 |
| EcoRI | GAATTC | 1 cut(s) | 2569 |
| EcoRII | CCWGG | 4 cut(s) | 538, 870, 1222, 1464 |
| EcoRV | GATATC | 1 cut(s) | 1272 |
| EcoT14I | CCWWGG | 3 cut(s) | 757, 771, 1451 |
| EcoT22I | ATGCAT | 1 cut(s) | 1280 |
| EcoT38I | GRGCYC | 1 cut(s) | 150 |
| ErhI | CCWWGG | 3 cut(s) | 757, 771, 1451 |
| FaqI | GGGAC | 2 cut(s) | 1201, 2418 |
| FbaI | TGATCA | 1 cut(s) | 1342 |
| FblI | GTMKAC | 1 cut(s) | 170 |
| Fnu4HI | GCNGC | 8 cut(s) | 306, 840, 1586, 1917, 1964, 2016, 2202, 2828 |
| FriOI | GRGCYC | 1 cut(s) | 150 |
| Fsp4HI | GCNGC | 8 cut(s) | 306, 840, 1586, 1917, 1964, 2016, 2202, 2828 |
| FspBI | CTAG | 6 cut(s) | 707, 1131, 1568, 2148, 2852, 2862 |
| GluI | GCNGC | 8 cut(s) | 306, 840, 1586, 1917, 1964, 2016, 2202, 2828 |
| GsuI | CTGGAG | 3 cut(s) | 534, 803, 2825 |
| HaeIII | GGCC | 4 cut(s) | 140, 232, 769, 2978 |
| HapII | CCGG | 5 cut(s) | 143, 206, 1671, 1992, 2666 |
| HgaI | GACGC | 2 cut(s) | 340, 2810 |
| Hin1I | GRCGYC | 1 cut(s) | 351 |
| HincII | GTYRAC | 2 cut(s) | 550, 789 |
| HindII | GTYRAC | 2 cut(s) | 550, 789 |
| HindIII | AAGCTT | 2 cut(s) | 2459, 2514 |
| HpaII | CCGG | 5 cut(s) | 143, 206, 1671, 1992, 2666 |
| HphI | GGTGA | 5 cut(s) | 195, 554, 647, 2237, 2507 |
| Hpy166II | GTNNAC | 5 cut(s) | 171, 457, 550, 611, 789 |
| Hpy8I | GTNNAC | 5 cut(s) | 171, 457, 550, 611, 789 |
| Hpy99I | CGWCG | 1 cut(s) | 987 |
| HpyAV | CCTTC | 8 cut(s) | 901, 981, 1082, 1267, 1279, 1790, 1844, 2465 |
| HpyCH4IV | ACGT | 3 cut(s) | 982, 1351, 1750 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 836, 1667, 2024, 2184, 2754, 2827 |
| HpySE526I | ACGT | 3 cut(s) | 982, 1351, 1750 |
| Hsp92I | GRCGYC | 1 cut(s) | 351 |
| Ksp22I | TGATCA | 1 cut(s) | 1342 |
| LmnI | GCTCC | 6 cut(s) | 145, 1259, 1934, 1960, 2321, 2490 |
| Lsp1109I | GCAGC | 7 cut(s) | 292, 851, 1572, 1928, 1975, 2027, 2188 |
| LweI | GCATC | 7 cut(s) | 307, 334, 492, 561, 1048, 1387, 2149 |
| MabI | ACCWGGT | 1 cut(s) | 1222 |
| MaeI | CTAG | 6 cut(s) | 707, 1131, 1568, 2148, 2852, 2862 |
| MaeII | ACGT | 3 cut(s) | 982, 1351, 1750 |
| MaeIII | GTNAC | 7 cut(s) | 31, 1121, 1852, 1901, 2243, 2679, 2701 |
| MfeI | CAATTG | 1 cut(s) | 2414 |
| MflI | RGATCY | 3 cut(s) | 625, 1021, 2551 |
| MhlI | GDGCHC | 2 cut(s) | 46, 150 |
| MlyI | GAGTC | 5 cut(s) | 794, 929, 2512, 2528, 2723 |
| MmeI | TCCRAC | 3 cut(s) | 983, 1666, 3015 |
| Mph1103I | ATGCAT | 1 cut(s) | 1280 |
| MroXI | GAANNNNTTC | 3 cut(s) | 396, 522, 1289 |
| MseI | TTAA | 7 cut(s) | 1242, 1889, 2043, 2166, 2271, 2463, 2616 |
| MslI | CAYNNNNRTG | 5 cut(s) | 381, 651, 1107, 1350, 2008 |
| MspA1I | CMGCKG | 1 cut(s) | 2018 |
| MspCI | CTTAAG | 1 cut(s) | 2165 |
| MspI | CCGG | 5 cut(s) | 143, 206, 1671, 1992, 2666 |
| MspR9I | CCNGG | 5 cut(s) | 143, 540, 872, 1224, 1466 |
| MunI | CAATTG | 1 cut(s) | 2414 |
| Mva1269I | GAATGC | 3 cut(s) | 869, 2366, 2929 |
| MvaI | CCWGG | 4 cut(s) | 540, 872, 1224, 1466 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 836, 1667, 2024, 2184, 2754, 2827 |
| NciI | CCSGG | 1 cut(s) | 143 |
| NlaIV | GGNNCC | 4 cut(s) | 141, 147, 603, 627 |
| NmuCI | GTSAC | 3 cut(s) | 31, 2243, 2679 |
| NsiI | ATGCAT | 1 cut(s) | 1280 |
| NspI | RCATGY | 3 cut(s) | 650, 1775, 2385 |
| OliI | CACNNNNGTG | 1 cut(s) | 2008 |
| PaeR7I | CTCGAG | 1 cut(s) | 2536 |
| PagI | TCATGA | 2 cut(s) | 1492, 2214 |
| PaqCI | CACCTGC | 1 cut(s) | 89 |
| PceI | AGGCCT | 1 cut(s) | 232 |
| PctI | GAATGC | 3 cut(s) | 869, 2366, 2929 |
| PdmI | GAANNNNTTC | 3 cut(s) | 396, 522, 1289 |
| PflFI | GACNNNGTC | 1 cut(s) | 328 |
| PinAI | ACCGGT | 1 cut(s) | 2665 |
| PkrI | GCNGC | 8 cut(s) | 307, 841, 1587, 1918, 1965, 2017, 2203, 2829 |
| PleI | GAGTC | 5 cut(s) | 793, 929, 2511, 2528, 2723 |
| PmaCI | CACGTG | 1 cut(s) | 1352 |
| PmlI | CACGTG | 1 cut(s) | 1352 |
| PpsI | GAGTC | 5 cut(s) | 793, 929, 2511, 2528, 2723 |
| Ppu21I | YACGTR | 1 cut(s) | 1352 |
| PshBI | ATTAAT | 1 cut(s) | 1889 |
| Psp6I | CCWGG | 4 cut(s) | 538, 870, 1222, 1464 |
| PspCI | CACGTG | 1 cut(s) | 1352 |
| PspEI | GGTNACC | 1 cut(s) | 2243 |
| PspGI | CCWGG | 4 cut(s) | 538, 870, 1222, 1464 |
| PspN4I | GGNNCC | 4 cut(s) | 141, 147, 603, 627 |
| PspPI | GGNCC | 5 cut(s) | 139, 182, 467, 768, 1462 |
| PstI | CTGCAG | 1 cut(s) | 2444 |
| PstNI | CAGNNNCTG | 2 cut(s) | 35, 814 |
| PsuI | RGATCY | 3 cut(s) | 625, 1021, 2551 |
| PsyI | GACNNNGTC | 1 cut(s) | 328 |
| RsaI | GTAC | 3 cut(s) | 807, 1335, 2712 |
| RsaNI | GTAC | 3 cut(s) | 806, 1334, 2711 |
| RseI | CAYNNNNRTG | 5 cut(s) | 381, 651, 1107, 1350, 2008 |
| SaqAI | TTAA | 7 cut(s) | 1242, 1889, 2043, 2166, 2271, 2463, 2616 |
| SatI | GCNGC | 8 cut(s) | 306, 840, 1586, 1917, 1964, 2016, 2202, 2828 |
| Sau96I | GGNCC | 5 cut(s) | 139, 182, 467, 768, 1462 |
| SchI | GAGTC | 5 cut(s) | 794, 929, 2512, 2528, 2723 |
| ScrFI | CCNGG | 5 cut(s) | 143, 540, 872, 1224, 1466 |
| SduI | GDGCHC | 2 cut(s) | 46, 150 |
| SexAI | ACCWGGT | 1 cut(s) | 1222 |
| SfaNI | GCATC | 7 cut(s) | 307, 334, 492, 561, 1048, 1387, 2149 |
| SfcI | CTRYAG | 2 cut(s) | 171, 2440 |
| Sfr274I | CTCGAG | 1 cut(s) | 2536 |
| SinI | GGWCC | 3 cut(s) | 182, 467, 1462 |
| SlaI | CTCGAG | 1 cut(s) | 2536 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 381, 651, 1107, 1350, 2008 |
| SmlI | CTYRAG | 6 cut(s) | 1520, 2165, 2511, 2536, 3005, 3076 |
| SmoI | CTYRAG | 6 cut(s) | 1520, 2165, 2511, 2536, 3005, 3076 |
| SseBI | AGGCCT | 1 cut(s) | 232 |
| SsiI | CCGC | 4 cut(s) | 465, 2018, 2827, 2856 |
| SspMI | CTAG | 6 cut(s) | 707, 1131, 1568, 2148, 2852, 2862 |
| StuI | AGGCCT | 1 cut(s) | 232 |
| StyD4I | CCNGG | 5 cut(s) | 141, 538, 870, 1222, 1464 |
| StyI | CCWWGG | 3 cut(s) | 757, 771, 1451 |
| TaiI | ACGT | 3 cut(s) | 985, 1354, 1753 |
| TaqI | TCGA | 5 cut(s) | 371, 985, 1767, 1825, 2537 |
| TatI | WGTACW | 2 cut(s) | 805, 1333 |
| TauI | GCSGC | 1 cut(s) | 2830 |
| Tru1I | TTAA | 7 cut(s) | 1242, 1889, 2043, 2166, 2271, 2463, 2616 |
| Tru9I | TTAA | 7 cut(s) | 1242, 1889, 2043, 2166, 2271, 2463, 2616 |
| TscAI | CASTG | 9 cut(s) | 36, 68, 91, 123, 1132, 1912, 1951, 2406, 2632 |
| TseFI | GTSAC | 3 cut(s) | 31, 2243, 2679 |
| TseI | GCWGC | 7 cut(s) | 305, 839, 1585, 1916, 1963, 2015, 2201 |
| Tsp45I | GTSAC | 3 cut(s) | 31, 2243, 2679 |
| TspRI | CASTG | 9 cut(s) | 36, 68, 91, 123, 1132, 1912, 1951, 2406, 2632 |
| Tth111I | GACNNNGTC | 1 cut(s) | 328 |
| Vha464I | CTTAAG | 1 cut(s) | 2165 |
| VpaK11BI | GGWCC | 3 cut(s) | 182, 467, 1462 |
| VspI | ATTAAT | 1 cut(s) | 1889 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2587 |
| XceI | RCATGY | 3 cut(s) | 650, 1775, 2385 |
| XhoI | CTCGAG | 1 cut(s) | 2536 |
| XmiI | GTMKAC | 1 cut(s) | 170 |
| XmnI | GAANNNNTTC | 3 cut(s) | 396, 522, 1289 |
| XspI | CTAG | 6 cut(s) | 707, 1131, 1568, 2148, 2852, 2862 |
| Zsp2I | ATGCAT | 1 cut(s) | 1280 |