Prupe.1G030800_v2.0.a1

Zinc knuckle (CCHC-type) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001074
Physical Location & Seq
Reverse (-)
2189630 .. 2198222
8593 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G030800.13

Sequence Viewer

Length: 3171 bp
ATGGACGTGGACAATGAGAACATAGAACCTGGGACTGATTTAGGACTTGCTCTGGGATATTCGAATCAGTGCATTCAGAGAATATTGCATGGTGTTTCAGGTGCAGGTGCAAATGCAGGTTCGAGGATACACATGACATTTGTGGCCGCTGAGCCCCTGTCAGAATTAGTTTGGTCTAAAGACAAAGGTCCAAGCAATGTCACTCTTTCACCACCACAAAGCAATACAGGCGGGAGGTCTTCTACTGACAAGCCTATTGATGAAGAGAATTTTATAACACCACAAACATCATCGCATTTAAGGAGTGAAGCTGCCTGTAAAGATATGACGATGTCTCCCACAAGCGACGCTGGCATCATGCCTGCATGTGGGTCAAGTTGTGAACATGAGACGGGAACTGGTGGTAATGTGGAGGAAGTTAAAGCAGCAGTGGAAGTATCTGTTCCATATAATCAGGAGGGAATTTACCCTCCAGTCAATTTCCAAGTAGATGAAATACCTGAGACCAGAGAAAACGATTTTCCAACATTGTCAGGTAATGTTGACAGAGAAGGGGCTGATATCTTGTTAATTGAATCAGATCAAATACTACCTTTTGTGGAACAAAATGAACCATTATTGGGGGATCCTGATGGTGGAGACAGACATGCTGATGTTGGCAATCAGAAAATGGAAATGGATCTTGTTTCGACCTCTGAAGTTCACCCAGTGAATGAAAGTAAAGCTTCGGGTGCTCCAGTGGAGAATCAAAGGCCCCAAGGCAGAAGGCCTTTGGAAAAAATGGAGATAACTGCTGAGAATGATTTACAAAATCTCAAAAGTGAACATGCTTATGGTGCAGAGAGTCAGATTTTAGGATTAGAATCTTCCCCAGGGGTTAAAGACAAGTTTGAGCAGGATGTGGAAGTGCTTCCTGGAAACAAATCTGTTCTGGTTAAAGACTCTCCAACTAACAGTAAAATCCATAAATATCAATGGAAAGGCAAAGAAAAAGCGTTATCTTATGGAGATCTTAATGGAAGAATGTCAGAAGATGAGGATGATAGCCATGAGAGTGTTGAGAGCTGTAATAGTGCAGGGTTGTTTTCATTAGGAAAGAAGAGATGGAACTTTGAAGATGAGTTCATCGTTGGGAGTAAAAGATTCAGAAAGCAAATTCAAGAAACGCCGACTTGCATATCCTATATCAGACAAGATAGCTCCTTCATGAATTGGATGTCCAGCATGGTGAAGGGGTTTTCAAAATCAATGCAAGATGAGGCACCTTCTCTTGCTCTTACACTTGCACATCCTGATCATGGACATGCGCATTCTGATAAGAAGCTTATCACATGCAACAAGAACCAAGATGCTGGATTAAAAAATATCGGTTTCCAGTCAATTTTTCAGTCCTTGTATTGCCCAAAGGCAGAGCAGCAAGAAGCAAGGATGTTGAATGACAATCATCAAATAGGAGAAATATCAGCAGAACTTGAGTCAAATACCACTCCAAAAGCCTTTCATGGGGAGAAAATCAATTTAAGCAGAGTTTTGCTTTCAGTTGGGAAGTTCAAAAAATCTTCATCTGGAAATGAAGTACGTTCAGCAGCCCGAACTAAGAGTTCATCTGAAAAAGCAGCAGGTATTCAGGAGAAAGGCAACACTAACTCTGCAGAGGAAAAGAATCCATGCAACTTTCGGTTTCATAAAAACAAAGACAGAGCTAGCTCTAACTCTTCTCTGGGTAAACGCAAGAAAAAGAGTGTAGAGGATGTTGAATCTAGCCTACAATCTGAAGGGAAGACAACTGATAAATTTGGTCGTAGAAGTGCCCTTCTGGAAAGCTTGTGGATAACTCGATTTACTCAAAAAACACCTGCTCCATCATTAATCTTAAATCGATACATCCAGAGTACTGACGGAGTTCTTGAGTGCTCCGATGACCGCAAAAATGTGGGGGACAAGGAGCAGTCTGCTGAAGATTTAGTTATTGTCATTGGTAATGATCCGCAGAATTGTGTGGCTGACAATGAGGGCTCATCTGCTTTCAATAATAAAGGCCAGAATGATCAAAAGTCCATGTCCAAATTCAACCCTATCTTTCCTTCCCCTAAATTCAGAGGTTCAGAAGCAATGGCTTCATCTTTTGCTAGGAGATTGGATGCTCTGAAGCACATCACCCCATCTGGTGCAACAGGTAATGCCGCCTATGGAAACATGACCTGTTTCTTTTGTGGCAGAAAAGGTCACCATTTACGAGAGTGTTCAGAGATAACAGACACTGAACTTCAGGAATTACTCAGTAAATGCAAGTCCTATAATGGAGCTGAACATTTGCCTTCTTTCTGCATTAGATGTTCTCGATGCAGTCATTGGGCCACGGCATGTCCTAATGCGCCCTCAATGGGAGAATCTCAATTGGATTGTAATGTTTCTTGTTTAGATTATTACTGCAGTCAGAGTGAAATGAAGCACAATTCAAGAAATGATGTGAAGCTCCTAACTGGTAAGGAGAGTGAATTTCAATCTTCTGTTGCTCATACATTATTTGATGAGGATGATTCAAGAATAGAGGCAGATCTTAATTTGAGTTGGAAGACGAATAAGATGATAGTCTCGAAGAAAATGAGATCTCATCCAAATTCAGTTAAGGAATATAGTTCTTCAAGCCTTGGGGAAAATAAGCTAATGCCCTTGTCAAAGTTTGTCAATGCACAGATTTCAGATGTACCGAAAGGAATCTTTGACTCTGTAAGAAGGCTTCGCTTGTCTCGCACAGATGTCGTGAAATGGATGAATTCCCATACGTCACTCTCACAACTTGAGGGGTTTTTCCTGCGCCTGCGGCTTGGGAAGTGGGAAGAAGGACTTGGGGGAACTGGATACTATGTGTCTTGCATAACTGGTTCTCAGAGAGAGACTTGTCCACAGAATGTGGACTCTATAGCTGTTGTTGTTGGGGGAATCAAATGTTTGGTCAAGAGCCAATATGTGTCAAACCATGATTTCCTTGAGGATGAGCTGAAGGCATGGTGGTCTGCGACCTCAAAGGGCAATGGCAAGCTTCCATCTGAAGAAGATTTGAGAGAACAAGTGAAGAGAAAAACAATGTTAGGGCTACCTGCAGCTGAAACTAGGAATTGTAGTCAACTAAAGATTATATACGAAAGACTTCAAGGGATATATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plus-3

Protein Analysis

1057

Amino Acids

116.6

Weight (kDa)

5.85

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 275
AarI CACCTGC 2 cut(s) 95, 1864
Acc16I TGCGCA 1 cut(s) 1308
Acc36I ACCTGC 5 cut(s) 95, 107, 1610, 1864, 3109
AccB1I GGYRCC 1 cut(s) 1261
AciI CCGC 6 cut(s) 147, 231, 1924, 1988, 2184, 2824
AclWI GGATC 4 cut(s) 620, 633, 687, 1979
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 9 cut(s) 268, 462, 1155, 1793, 2066, 2093, 2498, 2620, 2776
AcuI CTGAAG 7 cut(s) 717, 1794, 1977, 2168, 2252, 3023, 3072
AdeI CACNNNGTG 1 cut(s) 709
AfaI GTAC 3 cut(s) 1578, 1894, 2709
AfiI CCNNNNNNNGG 6 cut(s) 368, 620, 635, 1298, 1503, 2384
AjiI CACGTC 1 cut(s) 7
AjnI CCWGG 3 cut(s) 28, 871, 913
AjuI GAANNNNNNNTTGG 2 cut(s) 2832, 2864
Alw21I GWGCWC 2 cut(s) 736, 1916
Alw26I GTCTC 7 cut(s) 339, 383, 497, 633, 2599, 2754, 2891
AlwI GGATC 4 cut(s) 620, 633, 687, 1979
AlwNI CAGNNNCTG 1 cut(s) 2261
AoxI GGCC 5 cut(s) 144, 752, 767, 2038, 2355
ApeKI GCWGC 6 cut(s) 311, 425, 1414, 1586, 1616, 3104
ApoI RAATTY 9 cut(s) 268, 462, 1155, 1793, 2066, 2093, 2498, 2620, 2776
ArsI GACNNNNNNTTYG 2 cut(s) 2940, 2972
AseI ATTAAT 1 cut(s) 1868
Asp700I GAANNNNTTC 2 cut(s) 909, 3150
AspLEI GCGC 3 cut(s) 1309, 2377, 2820
AspS9I GGNCC 3 cut(s) 188, 753, 2355
AsuHPI GGTGA 5 cut(s) 201, 695, 1240, 2149, 2219
AsuII TTCGAA 1 cut(s) 62
AsuNHI GCTAGC 1 cut(s) 1703
AvaII GGWCC 1 cut(s) 188
BaeGI GKGCMC 1 cut(s) 1813
BamHI GGATCC 1 cut(s) 625
BanI GGYRCC 1 cut(s) 1261
BanII GRGCYC 2 cut(s) 156, 2018
BarI GAAGNNNNNNTAC 2 cut(s) 2724, 2756
BbsI GAAGAC 3 cut(s) 231, 1787, 2582
Bbv12I GWGCWC 2 cut(s) 736, 1916
BbvI GCAGC 6 cut(s) 298, 437, 1426, 1598, 1628, 3116
BccI CCATC 5 cut(s) 626, 1098, 1870, 2170, 3055
BceAI ACGGC 1 cut(s) 2376
BcgI CGANNNNNNTGC 2 cut(s) 2743, 2777
BciT130I CCWGG 3 cut(s) 30, 873, 915
BciVI GTATCC 2 cut(s) 120, 2855
BclI TGATCA 2 cut(s) 1294, 2047
BcoDI GTCTC 7 cut(s) 339, 383, 497, 633, 2599, 2754, 2891
BfaI CTAG 4 cut(s) 1704, 1761, 2130, 3114
BfmI CTRYAG 4 cut(s) 1650, 2431, 2922, 3102
BfuAI ACCTGC 5 cut(s) 95, 107, 1610, 1864, 3109
BfuI GTATCC 2 cut(s) 120, 2855
BglII AGATCT 3 cut(s) 1009, 2557, 2609
BisI GCNGC 9 cut(s) 147, 312, 426, 1415, 1587, 1617, 2184, 2825, 3105
BlpI GCTNAGC 1 cut(s) 150
BlsI GCNGC 9 cut(s) 148, 313, 427, 1416, 1588, 1618, 2185, 2826, 3106
BmcAI AGTACT 1 cut(s) 1894
Bme1390I CCNGG 3 cut(s) 30, 873, 915
Bme18I GGWCC 1 cut(s) 188
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 3 cut(s) 188, 753, 2355
BmiI GGNNCC 3 cut(s) 627, 755, 1263
BmrFI CCNGG 3 cut(s) 30, 873, 915
BmrI ACTGGG 1 cut(s) 701
BmsI GCATC 4 cut(s) 363, 1339, 2131, 2333
BmtI GCTAGC 1 cut(s) 1707
BmuI ACTGGG 1 cut(s) 701
BoxI GACNNNNGTC 1 cut(s) 186
BpiI GAAGAC 3 cut(s) 231, 1787, 2582
BpmI CTGGAG 2 cut(s) 456, 720
Bpu1102I GCTNAGC 1 cut(s) 150
Bpu14I TTCGAA 1 cut(s) 62
BpuEI CTTGAG 4 cut(s) 1493, 1928, 2822, 3011
Bsa29I ATCGAT 1 cut(s) 1879
BsaBI GATNNNNATC 1 cut(s) 862
BsaI GGTCTC 1 cut(s) 497
BsaJI CCNNGG 6 cut(s) 29, 757, 871, 872, 2358, 2650
BsaXI ACNNNNNCTCC 4 cut(s) 319, 349, 1843, 1873
Bsc4I CCNNNNNNNGG 6 cut(s) 368, 620, 635, 1298, 1503, 2384
Bse1I ACTGG 8 cut(s) 403, 473, 707, 737, 1375, 2488, 2863, 2887
Bse3DI GCAATG 3 cut(s) 202, 2118, 3040
Bse8I GATNNNNATC 1 cut(s) 862
BseBI CCWGG 3 cut(s) 30, 873, 915
BseCI ATCGAT 1 cut(s) 1879
BseDI CCNNGG 6 cut(s) 29, 757, 871, 872, 2358, 2650
BseJI GATNNNNATC 1 cut(s) 862
BseLI CCNNNNNNNGG 6 cut(s) 368, 620, 635, 1298, 1503, 2384
BseMI GCAATG 3 cut(s) 202, 2118, 3040
BseMII CTCAG 5 cut(s) 141, 492, 786, 2293, 2903
BseNI ACTGG 8 cut(s) 403, 473, 707, 737, 1375, 2488, 2863, 2887
BseSI GKGCMC 1 cut(s) 1813
BseXI GCAGC 6 cut(s) 298, 437, 1426, 1598, 1628, 3116
BsgI GTGCAG 3 cut(s) 123, 858, 1095
BshFI GGCC 5 cut(s) 146, 754, 769, 2040, 2357
BshNI GGYRCC 1 cut(s) 1261
BshVI ATCGAT 1 cut(s) 1879
BsiHKAI GWGCWC 2 cut(s) 736, 1916
BslFI GGGAC 2 cut(s) 46, 1952
BslI CCNNNNNNNGG 6 cut(s) 368, 620, 635, 1298, 1503, 2384
BsmAI GTCTC 7 cut(s) 339, 383, 497, 633, 2599, 2754, 2891
BsmBI CGTCTC 1 cut(s) 383
BsmFI GGGAC 2 cut(s) 46, 1952
BsmI GAATGC 2 cut(s) 72, 1309
BsnI GGCC 5 cut(s) 146, 754, 769, 2040, 2357
Bso31I GGTCTC 1 cut(s) 497
Bsp119I TTCGAA 1 cut(s) 62
Bsp1286I GDGCHC 5 cut(s) 156, 736, 1813, 1916, 2018
Bsp143I GATC 9 cut(s) 580, 625, 679, 1009, 1294, 1984, 2047, 2557, 2609
Bsp1720I GCTNAGC 1 cut(s) 150
BspACI CCGC 6 cut(s) 147, 231, 1924, 1988, 2184, 2824
BspANI GGCC 5 cut(s) 146, 754, 769, 2040, 2357
BspCNI CTCAG 5 cut(s) 142, 493, 787, 2292, 2902
BspDI ATCGAT 1 cut(s) 1879
BspHI TCATGA 1 cut(s) 1206
BspLI GGNNCC 3 cut(s) 627, 755, 1263
BspMAI CTGCAG 3 cut(s) 1654, 2435, 3106
BspMI ACCTGC 5 cut(s) 95, 107, 1610, 1864, 3109
BspOI GCTAGC 1 cut(s) 1707
BspPI GGATC 4 cut(s) 620, 633, 687, 1979
BspT104I TTCGAA 1 cut(s) 62
BspT107I GGYRCC 1 cut(s) 1261
BspTNI GGTCTC 1 cut(s) 497
BsrDI GCAATG 3 cut(s) 202, 2118, 3040
BsrI ACTGG 8 cut(s) 403, 473, 707, 737, 1375, 2488, 2863, 2887
BssECI CCNNGG 6 cut(s) 29, 757, 871, 872, 2358, 2650
BssMI GATC 9 cut(s) 580, 625, 679, 1009, 1294, 1984, 2047, 2557, 2609
BssT1I CCWWGG 2 cut(s) 757, 2650
Bst2UI CCWGG 3 cut(s) 30, 873, 915
Bst4CI ACNGT 1 cut(s) 956
Bst6I CTCTTC 4 cut(s) 258, 1094, 1720, 3071
BstBI TTCGAA 1 cut(s) 62
BstC8I GCNNGC 5 cut(s) 352, 363, 1705, 2822, 3041
BstDEI CTNAG 6 cut(s) 150, 501, 795, 1596, 2279, 2889
BstDSI CCRYGG 1 cut(s) 2358
BstEII GGTNACC 1 cut(s) 2225
BstHHI GCGC 3 cut(s) 1309, 2377, 2820
BstKTI GATC 9 cut(s) 583, 628, 682, 1012, 1297, 1987, 2050, 2560, 2612
BstMAI GTCTC 7 cut(s) 339, 383, 497, 633, 2599, 2754, 2891
BstMBI GATC 9 cut(s) 580, 625, 679, 1009, 1294, 1984, 2047, 2557, 2609
BstMWI GCNNNNNNNGC 6 cut(s) 228, 351, 731, 836, 2751, 2824
BstNI CCWGG 3 cut(s) 30, 873, 915
BstNSI RCATGY 6 cut(s) 369, 650, 830, 1307, 1335, 2367
BstPAI GACNNNNGTC 1 cut(s) 186
BstPI GGTNACC 1 cut(s) 2225
BstSCI CCNGG 3 cut(s) 28, 871, 913
BstSFI CTRYAG 4 cut(s) 1650, 2431, 2922, 3102
BstSLI GKGCMC 1 cut(s) 1813
BstV1I GCAGC 6 cut(s) 298, 437, 1426, 1598, 1628, 3116
BstV2I GAAGAC 3 cut(s) 231, 1787, 2582
BstX2I RGATCY 5 cut(s) 625, 679, 1009, 2557, 2609
BstXI CCANNNNNNTGG 1 cut(s) 1352
BstYI RGATCY 5 cut(s) 625, 679, 1009, 2557, 2609
Bsu15I ATCGAT 1 cut(s) 1879
BsuI GTATCC 2 cut(s) 120, 2855
BsuRI GGCC 5 cut(s) 146, 754, 769, 2040, 2357
BsuTUI ATCGAT 1 cut(s) 1879
BtgI CCRYGG 1 cut(s) 2358
BtgZI GCGATG 1 cut(s) 276
BtrI CACGTC 1 cut(s) 7
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 5 cut(s) 74, 435, 714, 744, 2259
BveI ACCTGC 5 cut(s) 95, 107, 1610, 1864, 3109
Cac8I GCNNGC 5 cut(s) 352, 363, 1705, 2822, 3041
CaiI CAGNNNCTG 1 cut(s) 2261
CciI TCATGA 1 cut(s) 1206
CfoI GCGC 3 cut(s) 1309, 2377, 2820
Cfr13I GGNCC 3 cut(s) 188, 753, 2355
ClaI ATCGAT 1 cut(s) 1879
CseI GACGC 1 cut(s) 356
Csp6I GTAC 3 cut(s) 1577, 1893, 2708
CviQI GTAC 3 cut(s) 1577, 1893, 2708
DdeI CTNAG 6 cut(s) 150, 501, 795, 1596, 2279, 2889
DpnI GATC 9 cut(s) 582, 627, 681, 1011, 1296, 1986, 2049, 2559, 2611
DpnII GATC 9 cut(s) 580, 625, 679, 1009, 1294, 1984, 2047, 2557, 2609
DraIII CACNNNGTG 1 cut(s) 709
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 4 cut(s) 258, 1094, 1720, 3071
EarI CTCTTC 4 cut(s) 258, 1094, 1720, 3071
Eco130I CCWWGG 2 cut(s) 757, 2650
Eco147I AGGCCT 1 cut(s) 769
Eco24I GRGCYC 2 cut(s) 156, 2018
Eco31I GGTCTC 1 cut(s) 497
Eco32I GATATC 1 cut(s) 562
Eco47I GGWCC 1 cut(s) 188
Eco57I CTGAAG 7 cut(s) 717, 1794, 1977, 2168, 2252, 3023, 3072
Eco91I GGTNACC 1 cut(s) 2225
EcoO109I RGGNCCY 1 cut(s) 753
EcoO65I GGTNACC 1 cut(s) 2225
EcoRI GAATTC 1 cut(s) 2776
EcoRII CCWGG 3 cut(s) 28, 871, 913
EcoRV GATATC 1 cut(s) 562
EcoT14I CCWWGG 2 cut(s) 757, 2650
EcoT38I GRGCYC 2 cut(s) 156, 2018
ErhI CCWWGG 2 cut(s) 757, 2650
Esp3I CGTCTC 1 cut(s) 383
FalI AAGNNNNNCTT 4 cut(s) 709, 741, 2832, 2864
FaqI GGGAC 2 cut(s) 46, 1952
FauI CCCGC 1 cut(s) 224
FbaI TGATCA 2 cut(s) 1294, 2047
Fnu4HI GCNGC 9 cut(s) 147, 312, 426, 1415, 1587, 1617, 2184, 2825, 3105
FriOI GRGCYC 2 cut(s) 156, 2018
Fsp4HI GCNGC 9 cut(s) 147, 312, 426, 1415, 1587, 1617, 2184, 2825, 3105
FspAI RTGCGCAY 1 cut(s) 1308
FspBI CTAG 4 cut(s) 1704, 1761, 2130, 3114
FspI TGCGCA 1 cut(s) 1308
GlaI GCGC 3 cut(s) 1308, 2376, 2819
GluI GCNGC 9 cut(s) 147, 312, 426, 1415, 1587, 1617, 2184, 2825, 3105
GsuI CTGGAG 2 cut(s) 456, 720
HaeIII GGCC 5 cut(s) 146, 754, 769, 2040, 2357
HgaI GACGC 1 cut(s) 356
HhaI GCGC 3 cut(s) 1309, 2377, 2820
Hin6I GCGC 3 cut(s) 1307, 2375, 2818
HinP1I GCGC 3 cut(s) 1307, 2375, 2818
HincII GTYRAC 2 cut(s) 544, 3128
HindII GTYRAC 2 cut(s) 544, 3128
HindIII AAGCTT 4 cut(s) 723, 1322, 1822, 3041
HphI GGTGA 5 cut(s) 201, 695, 1240, 2149, 2219
Hpy166II GTNNAC 9 cut(s) 10, 383, 544, 703, 824, 1727, 2906, 2917, 3128
Hpy8I GTNNAC 9 cut(s) 10, 383, 544, 703, 824, 1727, 2906, 2917, 3128
Hpy99I CGWCG 1 cut(s) 350
HpyCH4III ACNGT 1 cut(s) 956
HpyCH4IV ACGT 3 cut(s) 6, 1579, 2786
HpyF10VI GCNNNNNNNGC 6 cut(s) 228, 351, 731, 836, 2751, 2824
HpyF3I CTNAG 6 cut(s) 150, 501, 795, 1596, 2279, 2889
HpySE526I ACGT 3 cut(s) 6, 1579, 2786
HspAI GCGC 3 cut(s) 1307, 2375, 2818
Ksp22I TGATCA 2 cut(s) 1294, 2047
Kzo9I GATC 9 cut(s) 580, 625, 679, 1009, 1294, 1984, 2047, 2557, 2609
LmnI GCTCC 7 cut(s) 739, 1205, 1864, 1919, 1945, 2303, 2481
Lsp1109I GCAGC 6 cut(s) 298, 437, 1426, 1598, 1628, 3116
LweI GCATC 4 cut(s) 363, 1339, 2131, 2333
MaeI CTAG 4 cut(s) 1704, 1761, 2130, 3114
MaeII ACGT 3 cut(s) 6, 1579, 2786
MaeIII GTNAC 3 cut(s) 199, 2225, 2787
MalI GATC 9 cut(s) 582, 627, 681, 1011, 1296, 1986, 2049, 2559, 2611
MboI GATC 9 cut(s) 580, 625, 679, 1009, 1294, 1984, 2047, 2557, 2609
MfeI CAATTG 1 cut(s) 2396
MflI RGATCY 5 cut(s) 625, 679, 1009, 2557, 2609
MhlI GDGCHC 5 cut(s) 156, 736, 1813, 1916, 2018
MlyI GAGTC 5 cut(s) 853, 935, 1484, 2720, 2912
MmeI TCCRAC 3 cut(s) 548, 971, 2552
MroXI GAANNNNTTC 2 cut(s) 909, 3150
MslI CAYNNNNRTG 4 cut(s) 651, 831, 1053, 1302
MspA1I CMGCKG 2 cut(s) 149, 3107
MspR9I CCNGG 3 cut(s) 30, 873, 915
MunI CAATTG 1 cut(s) 2396
Mva1269I GAATGC 2 cut(s) 72, 1309
MvaI CCWGG 3 cut(s) 30, 873, 915
MwoI GCNNNNNNNGC 6 cut(s) 228, 351, 731, 836, 2751, 2824
NdeII GATC 9 cut(s) 580, 625, 679, 1009, 1294, 1984, 2047, 2557, 2609
NheI GCTAGC 1 cut(s) 1703
NlaIV GGNNCC 3 cut(s) 627, 755, 1263
NmuCI GTSAC 3 cut(s) 199, 2225, 2787
NsbI TGCGCA 1 cut(s) 1308
NspI RCATGY 6 cut(s) 369, 650, 830, 1307, 1335, 2367
NspV TTCGAA 1 cut(s) 62
PagI TCATGA 1 cut(s) 1206
PaqCI CACCTGC 2 cut(s) 95, 1864
PasI CCCWGGG 1 cut(s) 872
PceI AGGCCT 1 cut(s) 769
PctI GAATGC 2 cut(s) 72, 1309
PdmI GAANNNNTTC 2 cut(s) 909, 3150
PflFI GACNNNGTC 1 cut(s) 331
PfoI TCCNGGA 1 cut(s) 913
PkrI GCNGC 9 cut(s) 148, 313, 427, 1416, 1588, 1618, 2185, 2826, 3106
PleI GAGTC 5 cut(s) 852, 935, 1483, 2720, 2912
PpsI GAGTC 5 cut(s) 852, 935, 1483, 2720, 2912
PshAI GACNNNNGTC 1 cut(s) 186
PshBI ATTAAT 1 cut(s) 1868
PsiI TTATAA 1 cut(s) 275
Psp6I CCWGG 3 cut(s) 28, 871, 913
PspEI GGTNACC 1 cut(s) 2225
PspGI CCWGG 3 cut(s) 28, 871, 913
PspN4I GGNNCC 3 cut(s) 627, 755, 1263
PspPI GGNCC 3 cut(s) 188, 753, 2355
PstI CTGCAG 3 cut(s) 1654, 2435, 3106
PstNI CAGNNNCTG 1 cut(s) 2261
PsuI RGATCY 5 cut(s) 625, 679, 1009, 2557, 2609
PsyI GACNNNGTC 1 cut(s) 331
PvuII CAGCTG 1 cut(s) 3107
RsaI GTAC 3 cut(s) 1578, 1894, 2709
RsaNI GTAC 3 cut(s) 1577, 1893, 2708
RseI CAYNNNNRTG 4 cut(s) 651, 831, 1053, 1302
SatI GCNGC 9 cut(s) 147, 312, 426, 1415, 1587, 1617, 2184, 2825, 3105
Sau3AI GATC 9 cut(s) 580, 625, 679, 1009, 1294, 1984, 2047, 2557, 2609
Sau96I GGNCC 3 cut(s) 188, 753, 2355
ScaI AGTACT 1 cut(s) 1894
SchI GAGTC 5 cut(s) 853, 935, 1484, 2720, 2912
ScrFI CCNGG 3 cut(s) 30, 873, 915
SduI GDGCHC 5 cut(s) 156, 736, 1813, 1916, 2018
SfaNI GCATC 4 cut(s) 363, 1339, 2131, 2333
SfcI CTRYAG 4 cut(s) 1650, 2431, 2922, 3102
SfuI TTCGAA 1 cut(s) 62
SinI GGWCC 1 cut(s) 188
SmiMI CAYNNNNRTG 4 cut(s) 651, 831, 1053, 1302
SmlI CTYRAG 4 cut(s) 1472, 1907, 2801, 2990
SmoI CTYRAG 4 cut(s) 1472, 1907, 2801, 2990
SseBI AGGCCT 1 cut(s) 769
SsiI CCGC 6 cut(s) 147, 231, 1924, 1988, 2184, 2824
SspI AATATT 1 cut(s) 84
SspMI CTAG 4 cut(s) 1704, 1761, 2130, 3114
StuI AGGCCT 1 cut(s) 769
StyD4I CCNGG 3 cut(s) 28, 871, 913
StyI CCWWGG 2 cut(s) 757, 2650
TaaI ACNGT 1 cut(s) 956
TaiI ACGT 3 cut(s) 9, 1582, 2789
TaqI TCGA 7 cut(s) 62, 122, 689, 1837, 1879, 2341, 2597
TatI WGTACW 1 cut(s) 1892
TauI GCSGC 3 cut(s) 149, 2186, 2827
TscAI CASTG 5 cut(s) 74, 435, 714, 744, 2266
TseFI GTSAC 3 cut(s) 199, 2225, 2787
TseI GCWGC 6 cut(s) 311, 425, 1414, 1586, 1616, 3104
Tsp45I GTSAC 3 cut(s) 199, 2225, 2787
TspGWI ACGGA 1 cut(s) 1914
TspRI CASTG 5 cut(s) 74, 435, 714, 744, 2266
Tth111I GACNNNGTC 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 188
VspI ATTAAT 1 cut(s) 1868
XapI RAATTY 9 cut(s) 268, 462, 1155, 1793, 2066, 2093, 2498, 2620, 2776
XceI RCATGY 6 cut(s) 369, 650, 830, 1307, 1335, 2367
XmnI GAANNNNTTC 2 cut(s) 909, 3150
XspI CTAG 4 cut(s) 1704, 1761, 2130, 3114
ZrmI AGTACT 1 cut(s) 1894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.