Zinc knuckle (CCHC-type) family protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 287, 1671 |
| AarI | CACCTGC | 1 cut(s) | 107 |
| Acc36I | ACCTGC | 3 cut(s) | 107, 119, 1595 |
| AccB1I | GGYRCC | 1 cut(s) | 1276 |
| AciI | CCGC | 3 cut(s) | 381, 431, 1985 |
| AclI | AACGTT | 1 cut(s) | 1527 |
| AclWI | GGATC | 4 cut(s) | 158, 635, 648, 1019 |
| AcsI | RAATTY | 6 cut(s) | 320, 529, 1006, 1170, 1544, 1778 |
| AcuI | CTGAAG | 3 cut(s) | 726, 1149, 1962 |
| AdeI | CACNNNGTG | 1 cut(s) | 724 |
| AfaI | GTAC | 1 cut(s) | 455 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 380, 397, 635, 1313 |
| AflIII | ACRYGT | 1 cut(s) | 1346 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 474 |
| AhlI | ACTAGT | 1 cut(s) | 58 |
| AjnI | CCWGG | 4 cut(s) | 459, 886, 928, 1925 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 686, 718 |
| AluBI | AGCT | 8 cut(s) | 740, 809, 1080, 1215, 1496, 1749, 1809, 1899 |
| AluI | AGCT | 8 cut(s) | 740, 809, 1080, 1215, 1496, 1749, 1809, 1899 |
| Alw21I | GWGCWC | 1 cut(s) | 1901 |
| Alw26I | GTCTC | 2 cut(s) | 351, 1046 |
| AlwI | GGATC | 4 cut(s) | 158, 635, 648, 1019 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 881 |
| Ama87I | CYCGRG | 1 cut(s) | 1487 |
| AoxI | GGCC | 4 cut(s) | 782, 1537, 1923, 2026 |
| ApeKI | GCWGC | 2 cut(s) | 254, 854 |
| ApoI | RAATTY | 6 cut(s) | 320, 529, 1006, 1170, 1544, 1778 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 942 |
| AspLEI | GCGC | 2 cut(s) | 1091, 2045 |
| AspS9I | GGNCC | 4 cut(s) | 872, 1795, 1844, 1923 |
| AsuHPI | GGTGA | 8 cut(s) | 116, 213, 569, 686, 710, 769, 1255, 1787 |
| AvaI | CYCGRG | 1 cut(s) | 1487 |
| AvaII | GGWCC | 3 cut(s) | 872, 1795, 1844 |
| BamHI | GGATCC | 1 cut(s) | 640 |
| BanI | GGYRCC | 1 cut(s) | 1276 |
| BanII | GRGCYC | 2 cut(s) | 1901, 2003 |
| BbsI | GAAGAC | 2 cut(s) | 1646, 1772 |
| Bbv12I | GWGCWC | 1 cut(s) | 1901 |
| BbvI | GCAGC | 2 cut(s) | 241, 866 |
| BccI | CCATC | 5 cut(s) | 916, 1013, 1361, 1855, 1877 |
| BciT130I | CCWGG | 4 cut(s) | 461, 888, 930, 1927 |
| BciVI | GTATCC | 2 cut(s) | 132, 181 |
| BclI | TGATCA | 3 cut(s) | 676, 907, 1309 |
| BcoDI | GTCTC | 2 cut(s) | 351, 1046 |
| BcuI | ACTAGT | 1 cut(s) | 58 |
| BfaI | CTAG | 3 cut(s) | 59, 797, 1746 |
| BfmI | CTRYAG | 1 cut(s) | 1422 |
| BfoI | RGCGCY | 1 cut(s) | 2046 |
| BfuAI | ACCTGC | 3 cut(s) | 107, 119, 1595 |
| BfuI | GTATCC | 2 cut(s) | 132, 181 |
| BglII | AGATCT | 1 cut(s) | 1124 |
| BisI | GCNGC | 4 cut(s) | 255, 432, 855, 1986 |
| BlsI | GCNGC | 4 cut(s) | 256, 433, 856, 1987 |
| Bme1390I | CCNGG | 4 cut(s) | 461, 888, 930, 1927 |
| Bme18I | GGWCC | 3 cut(s) | 872, 1795, 1844 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 474 |
| BmeT110I | CYCGRG | 1 cut(s) | 1487 |
| BmgT120I | GGNCC | 4 cut(s) | 872, 1795, 1844, 1923 |
| BmiI | GGNNCC | 6 cut(s) | 642, 927, 1278, 1633, 1796, 1924 |
| BmrFI | CCNGG | 4 cut(s) | 461, 888, 930, 1927 |
| BmrI | ACTGGG | 1 cut(s) | 716 |
| BmsI | GCATC | 1 cut(s) | 994 |
| BmuI | ACTGGG | 1 cut(s) | 716 |
| BpiI | GAAGAC | 2 cut(s) | 1646, 1772 |
| BpuEI | CTTGAG | 1 cut(s) | 1913 |
| Bsa29I | ATCGAT | 1 cut(s) | 2035 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1023, 1323 |
| BsaJI | CCNNGG | 3 cut(s) | 886, 887, 1926 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 331, 361 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 380, 397, 635, 1313 |
| Bse1I | ACTGG | 3 cut(s) | 722, 1633, 1777 |
| Bse3DI | GCAATG | 2 cut(s) | 214, 364 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1023, 1323 |
| BseBI | CCWGG | 4 cut(s) | 461, 888, 930, 1927 |
| BseCI | ATCGAT | 1 cut(s) | 2035 |
| BseDI | CCNNGG | 3 cut(s) | 886, 887, 1926 |
| BseGI | GGATG | 3 cut(s) | 1193, 1303, 1449 |
| BseJI | GATNNNNATC | 2 cut(s) | 1023, 1323 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 380, 397, 635, 1313 |
| BseMI | GCAATG | 2 cut(s) | 214, 364 |
| BseMII | CTCAG | 2 cut(s) | 521, 801 |
| BseNI | ACTGG | 3 cut(s) | 722, 1633, 1777 |
| BseXI | GCAGC | 2 cut(s) | 241, 866 |
| BsgI | GTGCAG | 2 cut(s) | 135, 873 |
| BshFI | GGCC | 4 cut(s) | 784, 1539, 1925, 2028 |
| BshNI | GGYRCC | 1 cut(s) | 1276 |
| BshVI | ATCGAT | 1 cut(s) | 2035 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1901 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1487 |
| BslFI | GGGAC | 5 cut(s) | 69, 481, 734, 869, 1808 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 380, 397, 635, 1313 |
| BsmAI | GTCTC | 2 cut(s) | 351, 1046 |
| BsmFI | GGGAC | 5 cut(s) | 69, 481, 734, 869, 1808 |
| BsmI | GAATGC | 3 cut(s) | 84, 149, 1044 |
| BsnI | GGCC | 4 cut(s) | 784, 1539, 1925, 2028 |
| BsoBI | CYCGRG | 1 cut(s) | 1487 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1901, 2003 |
| Bsp1407I | TGTACA | 1 cut(s) | 453 |
| BspACI | CCGC | 3 cut(s) | 381, 431, 1985 |
| BspANI | GGCC | 4 cut(s) | 784, 1539, 1925, 2028 |
| BspCNI | CTCAG | 2 cut(s) | 520, 802 |
| BspDI | ATCGAT | 1 cut(s) | 2035 |
| BspLI | GGNNCC | 6 cut(s) | 642, 927, 1278, 1633, 1796, 1924 |
| BspMAI | CTGCAG | 1 cut(s) | 1426 |
| BspMI | ACCTGC | 3 cut(s) | 107, 119, 1595 |
| BspPI | GGATC | 4 cut(s) | 158, 635, 648, 1019 |
| BspT107I | GGYRCC | 1 cut(s) | 1276 |
| BsrDI | GCAATG | 2 cut(s) | 214, 364 |
| BsrGI | TGTACA | 1 cut(s) | 453 |
| BsrI | ACTGG | 3 cut(s) | 722, 1633, 1777 |
| BssECI | CCNNGG | 3 cut(s) | 886, 887, 1926 |
| Bst2UI | CCWGG | 4 cut(s) | 461, 888, 930, 1927 |
| Bst4CI | ACNGT | 2 cut(s) | 406, 1554 |
| Bst6I | CTCTTC | 3 cut(s) | 417, 1109, 1128 |
| BstAUI | TGTACA | 1 cut(s) | 453 |
| BstC8I | GCNNGC | 2 cut(s) | 1494, 1807 |
| BstDEI | CTNAG | 2 cut(s) | 507, 810 |
| BstF5I | GGATG | 3 cut(s) | 1193, 1303, 1449 |
| BstH2I | RGCGCY | 1 cut(s) | 2046 |
| BstHHI | GCGC | 2 cut(s) | 1091, 2045 |
| BstMAI | GTCTC | 2 cut(s) | 351, 1046 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 155, 387, 851, 1086 |
| BstNI | CCWGG | 4 cut(s) | 461, 888, 930, 1927 |
| BstNSI | RCATGY | 3 cut(s) | 665, 845, 1350 |
| BstSCI | CCNGG | 4 cut(s) | 459, 886, 928, 1925 |
| BstSFI | CTRYAG | 1 cut(s) | 1422 |
| BstV1I | GCAGC | 2 cut(s) | 241, 866 |
| BstV2I | GAAGAC | 2 cut(s) | 1646, 1772 |
| BstX2I | RGATCY | 3 cut(s) | 640, 1024, 1124 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1367 |
| BstYI | RGATCY | 3 cut(s) | 640, 1024, 1124 |
| Bsu15I | ATCGAT | 1 cut(s) | 2035 |
| BsuI | GTATCC | 2 cut(s) | 132, 181 |
| BsuRI | GGCC | 4 cut(s) | 784, 1539, 1925, 2028 |
| BsuTUI | ATCGAT | 1 cut(s) | 2035 |
| BtsCI | GGATG | 3 cut(s) | 1193, 1303, 1449 |
| BtsIMutI | CAGTG | 1 cut(s) | 729 |
| BveI | ACCTGC | 3 cut(s) | 107, 119, 1595 |
| Cac8I | GCNNGC | 2 cut(s) | 1494, 1807 |
| CaiI | CAGNNNCTG | 1 cut(s) | 881 |
| CfoI | GCGC | 2 cut(s) | 1091, 2045 |
| Cfr13I | GGNCC | 4 cut(s) | 872, 1795, 1844, 1923 |
| ClaI | ATCGAT | 1 cut(s) | 2035 |
| Csp6I | GTAC | 1 cut(s) | 454 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 223, 258 |
| CviQI | GTAC | 1 cut(s) | 454 |
| DdeI | CTNAG | 2 cut(s) | 507, 810 |
| DraIII | CACNNNGTG | 1 cut(s) | 724 |
| DriI | GACNNNNNGTC | 1 cut(s) | 474 |
| Eam1104I | CTCTTC | 3 cut(s) | 417, 1109, 1128 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 474 |
| EarI | CTCTTC | 3 cut(s) | 417, 1109, 1128 |
| Ecl136II | GAGCTC | 1 cut(s) | 1899 |
| Eco147I | AGGCCT | 1 cut(s) | 784 |
| Eco24I | GRGCYC | 2 cut(s) | 1901, 2003 |
| Eco32I | GATATC | 2 cut(s) | 577, 1233 |
| Eco47I | GGWCC | 3 cut(s) | 872, 1795, 1844 |
| Eco53kI | GAGCTC | 1 cut(s) | 1899 |
| Eco57I | CTGAAG | 3 cut(s) | 726, 1149, 1962 |
| Eco88I | CYCGRG | 1 cut(s) | 1487 |
| EcoICRI | GAGCTC | 1 cut(s) | 1899 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1795 |
| EcoRII | CCWGG | 4 cut(s) | 459, 886, 928, 1925 |
| EcoRV | GATATC | 2 cut(s) | 577, 1233 |
| EcoT22I | ATGCAT | 2 cut(s) | 86, 987 |
| EcoT38I | GRGCYC | 2 cut(s) | 1901, 2003 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 724, 756 |
| FaqI | GGGAC | 5 cut(s) | 69, 481, 734, 869, 1808 |
| FauI | CCCGC | 1 cut(s) | 374 |
| FauNDI | CATATG | 1 cut(s) | 981 |
| FbaI | TGATCA | 3 cut(s) | 676, 907, 1309 |
| Fnu4HI | GCNGC | 4 cut(s) | 255, 432, 855, 1986 |
| FokI | GGATG | 3 cut(s) | 1180, 1290, 1456 |
| FriOI | GRGCYC | 2 cut(s) | 1901, 2003 |
| Fsp4HI | GCNGC | 4 cut(s) | 255, 432, 855, 1986 |
| FspBI | CTAG | 3 cut(s) | 59, 797, 1746 |
| GlaI | GCGC | 2 cut(s) | 1090, 2044 |
| GluI | GCNGC | 4 cut(s) | 255, 432, 855, 1986 |
| HaeII | RGCGCY | 1 cut(s) | 2046 |
| HaeIII | GGCC | 4 cut(s) | 784, 1539, 1925, 2028 |
| HhaI | GCGC | 2 cut(s) | 1091, 2045 |
| Hin6I | GCGC | 2 cut(s) | 1089, 2043 |
| HinP1I | GCGC | 2 cut(s) | 1089, 2043 |
| HincII | GTYRAC | 1 cut(s) | 1827 |
| HindII | GTYRAC | 1 cut(s) | 1827 |
| HindIII | AAGCTT | 2 cut(s) | 738, 1807 |
| HphI | GGTGA | 8 cut(s) | 116, 213, 569, 686, 710, 769, 1255, 1787 |
| Hpy166II | GTNNAC | 5 cut(s) | 718, 727, 1101, 1712, 1827 |
| Hpy8I | GTNNAC | 5 cut(s) | 718, 727, 1101, 1712, 1827 |
| HpyAV | CCTTC | 3 cut(s) | 1240, 1785, 1808 |
| HpyCH4III | ACNGT | 2 cut(s) | 406, 1554 |
| HpyCH4IV | ACGT | 4 cut(s) | 414, 1103, 1339, 1527 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 155, 387, 851, 1086 |
| HpyF3I | CTNAG | 2 cut(s) | 507, 810 |
| HpySE526I | ACGT | 4 cut(s) | 414, 1103, 1339, 1527 |
| HspAI | GCGC | 2 cut(s) | 1089, 2043 |
| Ksp22I | TGATCA | 3 cut(s) | 676, 907, 1309 |
| LmnI | GCTCC | 3 cut(s) | 1220, 1904, 1930 |
| Lsp1109I | GCAGC | 2 cut(s) | 241, 866 |
| LweI | GCATC | 1 cut(s) | 994 |
| MaeI | CTAG | 3 cut(s) | 59, 797, 1746 |
| MaeII | ACGT | 4 cut(s) | 414, 1103, 1339, 1527 |
| MaeIII | GTNAC | 6 cut(s) | 43, 211, 410, 557, 1451, 1964 |
| MflI | RGATCY | 3 cut(s) | 640, 1024, 1124 |
| MhlI | GDGCHC | 2 cut(s) | 1901, 2003 |
| MlyI | GAGTC | 2 cut(s) | 868, 1822 |
| MmeI | TCCRAC | 2 cut(s) | 862, 1893 |
| Mph1103I | ATGCAT | 2 cut(s) | 86, 987 |
| MroXI | GAANNNNTTC | 1 cut(s) | 942 |
| MseI | TTAA | 4 cut(s) | 821, 894, 1530, 1853 |
| MslI | CAYNNNNRTG | 3 cut(s) | 666, 1068, 1317 |
| MspA1I | CMGCKG | 1 cut(s) | 809 |
| MspR9I | CCNGG | 4 cut(s) | 461, 888, 930, 1927 |
| Mva1269I | GAATGC | 3 cut(s) | 84, 149, 1044 |
| MvaI | CCWGG | 4 cut(s) | 461, 888, 930, 1927 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 155, 387, 851, 1086 |
| NdeI | CATATG | 1 cut(s) | 981 |
| NlaIV | GGNNCC | 6 cut(s) | 642, 927, 1278, 1633, 1796, 1924 |
| NmuCI | GTSAC | 4 cut(s) | 43, 211, 557, 1451 |
| NsiI | ATGCAT | 2 cut(s) | 86, 987 |
| NspI | RCATGY | 3 cut(s) | 665, 845, 1350 |
| PaeR7I | CTCGAG | 1 cut(s) | 1487 |
| PaqCI | CACCTGC | 1 cut(s) | 107 |
| PasI | CCCWGGG | 1 cut(s) | 887 |
| PceI | AGGCCT | 1 cut(s) | 784 |
| PciI | ACATGT | 1 cut(s) | 1346 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 159 |
| PctI | GAATGC | 3 cut(s) | 84, 149, 1044 |
| PdmI | GAANNNNTTC | 1 cut(s) | 942 |
| PflFI | GACNNNGTC | 1 cut(s) | 343 |
| PfoI | TCCNGGA | 1 cut(s) | 928 |
| PkrI | GCNGC | 4 cut(s) | 256, 433, 856, 1987 |
| PleI | GAGTC | 2 cut(s) | 867, 1822 |
| PpsI | GAGTC | 2 cut(s) | 867, 1822 |
| PpuMI | RGGWCCY | 1 cut(s) | 1795 |
| PscI | ACATGT | 1 cut(s) | 1346 |
| PsiI | TTATAA | 2 cut(s) | 287, 1671 |
| Psp124BI | GAGCTC | 1 cut(s) | 1901 |
| Psp1406I | AACGTT | 1 cut(s) | 1527 |
| Psp5II | RGGWCCY | 1 cut(s) | 1795 |
| Psp6I | CCWGG | 4 cut(s) | 459, 886, 928, 1925 |
| PspGI | CCWGG | 4 cut(s) | 459, 886, 928, 1925 |
| PspN4I | GGNNCC | 6 cut(s) | 642, 927, 1278, 1633, 1796, 1924 |
| PspPI | GGNCC | 4 cut(s) | 872, 1795, 1844, 1923 |
| PspPPI | RGGWCCY | 1 cut(s) | 1795 |
| PspXI | VCTCGAGB | 1 cut(s) | 1487 |
| PstI | CTGCAG | 1 cut(s) | 1426 |
| PstNI | CAGNNNCTG | 1 cut(s) | 881 |
| PsuI | RGATCY | 3 cut(s) | 640, 1024, 1124 |
| PsyI | GACNNNGTC | 1 cut(s) | 343 |
| PvuII | CAGCTG | 1 cut(s) | 809 |
| RsaI | GTAC | 1 cut(s) | 455 |
| RsaNI | GTAC | 1 cut(s) | 454 |
| RseI | CAYNNNNRTG | 3 cut(s) | 666, 1068, 1317 |
| SacI | GAGCTC | 1 cut(s) | 1901 |
| SaqAI | TTAA | 4 cut(s) | 821, 894, 1530, 1853 |
| SatI | GCNGC | 4 cut(s) | 255, 432, 855, 1986 |
| Sau96I | GGNCC | 4 cut(s) | 872, 1795, 1844, 1923 |
| SchI | GAGTC | 2 cut(s) | 868, 1822 |
| ScrFI | CCNGG | 4 cut(s) | 461, 888, 930, 1927 |
| SduI | GDGCHC | 2 cut(s) | 1901, 2003 |
| SfaNI | GCATC | 1 cut(s) | 994 |
| SfcI | CTRYAG | 1 cut(s) | 1422 |
| Sfr274I | CTCGAG | 1 cut(s) | 1487 |
| SinI | GGWCC | 3 cut(s) | 872, 1795, 1844 |
| SlaI | CTCGAG | 1 cut(s) | 1487 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 666, 1068, 1317 |
| SmlI | CTYRAG | 2 cut(s) | 1487, 1892 |
| SmoI | CTYRAG | 2 cut(s) | 1487, 1892 |
| SpeI | ACTAGT | 1 cut(s) | 58 |
| SseBI | AGGCCT | 1 cut(s) | 784 |
| SsiI | CCGC | 3 cut(s) | 381, 431, 1985 |
| SspMI | CTAG | 3 cut(s) | 59, 797, 1746 |
| SstI | GAGCTC | 1 cut(s) | 1901 |
| StuI | AGGCCT | 1 cut(s) | 784 |
| StyD4I | CCNGG | 4 cut(s) | 459, 886, 928, 1925 |
| TaaI | ACNGT | 2 cut(s) | 406, 1554 |
| TaiI | ACGT | 4 cut(s) | 417, 1106, 1342, 1530 |
| TaqI | TCGA | 6 cut(s) | 489, 588, 1074, 1488, 1731, 2035 |
| TatI | WGTACW | 1 cut(s) | 453 |
| TauI | GCSGC | 2 cut(s) | 434, 1988 |
| Tru1I | TTAA | 4 cut(s) | 821, 894, 1530, 1853 |
| Tru9I | TTAA | 4 cut(s) | 821, 894, 1530, 1853 |
| TscAI | CASTG | 1 cut(s) | 729 |
| TseFI | GTSAC | 4 cut(s) | 43, 211, 557, 1451 |
| TseI | GCWGC | 2 cut(s) | 254, 854 |
| Tsp45I | GTSAC | 4 cut(s) | 43, 211, 557, 1451 |
| TspDTI | ATGAA | 9 cut(s) | 513, 523, 639, 666, 707, 1129, 1238, 1569, 1605 |
| TspGWI | ACGGA | 2 cut(s) | 1456, 1496 |
| TspRI | CASTG | 1 cut(s) | 729 |
| Tth111I | GACNNNGTC | 1 cut(s) | 343 |
| VpaK11BI | GGWCC | 3 cut(s) | 872, 1795, 1844 |
| XapI | RAATTY | 6 cut(s) | 320, 529, 1006, 1170, 1544, 1778 |
| XceI | RCATGY | 3 cut(s) | 665, 845, 1350 |
| XhoI | CTCGAG | 1 cut(s) | 1487 |
| XmnI | GAANNNNTTC | 1 cut(s) | 942 |
| XspI | CTAG | 3 cut(s) | 59, 797, 1746 |
| Zsp2I | ATGCAT | 2 cut(s) | 86, 987 |