MD13G1214600.v1.1

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006555
Physical Location & Seq
Forward (+)
20330598 .. 20336880
6283 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1214600.v1.1.491

Sequence Viewer

Length: 3234 bp
ATGGCAGATCCTGCTAGCTATGGGAATTATGAGCGTGATACTGAACAAGCACTCATTGCATTAAAAAAAGGTTCCCAGTTAATCAAGTATAGCCGGAAAGGACAGCCAAAACTTCATACATTCAGAATTTCTACTGATGAAACTACACTAATCTGGTATTCACATGGAGAAGAACGAACTCTGAAATTAGCTTCTGTCTCTCGAATTATCCCCGGACAGAGAACAGCTGTGTTTAGAAGGTATTTGCGCCCAGAAAAGGATTACTTGTCATTTTCTCTTCTATATAAGAACGGTGAACGAACACTTGATCTGATCTGCAAGGACAAAGCTGAGGCAGAGATATGGTTTGCTGGCCTTAAGGCGGTAATTACTTCAGGAAAAGAGCGTAGTAGACGTAGTCAGAGTGATATTTCTGATTTGCAAGACTCTGCTGAATCTGTTCATGGCCATCCATTTGGTGCGACATTAGAGTTCACGTCAAGCATTGCCAGTGGTAGGGTATCCGTTGATTCTCATGAATCAGTGAATTTGGCAGGCTCAGATGTGGGTTCAGAACGTGCAAACATGCAACTAAGAACAAGTGCAGGAGATGGTTTTCGAATTAGTGTTTCAAGCACTCCTAGCTGTTCAAGTGGAGGGTCTGGACATGATGATATAGAATCACTAGGAGATGTTTATGTGTGGGGAGAGATCTGGTCTGATGGAAATGGACCTGATGGGTCTGCAAATTCAATCTCTAAAAAAATCGATGTGCTGATTCCAAAGCCCTTGGAGTCGAATGTTGTTCTTGATGTTCAGCAGATAGCTTGTGGTGTGCGACATGTTGCTCTTGTAACAAGGCAAGGGGAGGTTTTCACCTGGGGAGAGGAATCGGGTGGAAGACTCGGTCATGGGATTGATAGAGACTTTAGTCGTCCTCGCCTTGTTGAGTTCCTCGCAATTAATAACATTGAATTTGTTGCATGTGGTGAGTACCACACATGTGCTGTATCTACATCAGGTGATTTGTTTACGTGGGGTGATGGTACACATAATGCTGGACTTCTTGGTCTTGGAACTGATGTTAGCCACTGGATACCTAAAAGGGTTACCGGTCCTTTAGAAGGACTTCAGGTTCTATCTGTTGCATGTGGCACATGGCATTCAGCTTTAGCAACTTCGAATGGAAAGGTATTTACATTTGGAGATGGAGCGTTCGGTGTTTTGGGCCATGGGGATCGAGAGAGTGTTCCGTATCCAAGGGAGGTACAGTCGTTGAATGGACTAAAGACTATAAAAGTAGCATGCGGAGTTTGGCATACTGCAGCTATTGTAGAAGTTATGGGCCAGTCTGGTACAAATGCTTCATCTCGTAAGTTGTTCACCTGGGGCGATGGTGACAAACATCGTTTGGGTCACGAAATCAAGGAAACTTATCTGCTTCCCACCTGTGTCTCTTCACTTATCGACTATAATTTCCACCAGCTAGCATGTGGACACACTATGACTATTGCCCTCACCACATCAGGTCATGTGTTTACCATGGGCGGAACTGCATATGGCCAGCTAGGCAATCCAAGTTCTGATGGGAAAGTACCTTGTTTAGTACAGGATCGATTGGTTGGTGAGTTTGTCGAAGAAATATCTTGTGGGTCATATCATGTTGCTGTCCTGACATCAAGAAGTGAAGTGTTCACTTGGGGAAAAGGTGCTAATGGAAGACTGGGACACGGGGACACAGAAGACCAGAAAACTCCAACTTTGGTTGAAGCCCTAAAAGATAGGCATGTGAAAAGCATATCGTGCGGCTCAAACTTCACATCTAGTATATGCATCCATAAGTGGATCTCTGGAGCTGATCAATCAATTTGCTCAGGTTGTCGACAGACATTTGGTTTTACTAGAAAGAGGCATAACTGTTATAACTGTGGACTAGTCCATTGCCATGCTTGTAGTTCCAAGAAAGCACTGAGGGCAGCTTTGGCCCCGACTCCTGGAAAACCTCATCGTGTATGTGATGCTTGTTATGCGAAACTTAAGGCTGCTGAGGCTGGTAATGCTTCCAGTGTTAGTAGGAGATCTAGGGTCACACGCTCGATGGATAGCAGGGATTTACTAAGTAGAGTAGAGGTGAAATCTTCAAGGATTCTTCTCTCTCCCTCTATAGAACCCGTTAAATACCTTGAGGTCAAGTCCATGAAGCCTGGTGGTGTCAGATCCGAATCCCCTTCTATAGTCAGGGCTTCCCAAGTTCCATCCCTTTTACAACTTAAAGATATTGCATTTCCAAGTTCGTTGAGTGCACTTCAAAACGTTTTGAAGCCTGTCATGACAACGCCGTCTCAGCCCAATTCGAGATCTACTTCACCATATTCAAGGAGACCTAGTCCTCCCCGGTCTACAACTCCTATATTCTCTAGGAGTGTTATTGACAGTCTTAAGAGAACAAATGGCACTCTGACTCAAGAAGTATCAAAGATGCAAAACCAGGTTAAAAGTCTGAAGCATAAGTGTGATGCTCAAGAGGTAGAGATAAAGAAACTACGTAAACATGTGAAAGAAGCTGGCTCATTAGCCGATGACCAATACTCGAAGTGCAGAGCTGCCAAAGAACTCGTCAAGTCCATCACGGAACAGATGAGGGAATGGGAAGACAAGATACCTCCTGAGATTTCTGACAGCGATACTTTCAAAGCATTGCGTACGCAAGCTGAGGATTTTATGAACACGAATATTGGAAGGCCAAGCTCAGATCTTGACCAACACTATGCAACCGATAGAACTTCCTTGGTTGTTGAGTCTTCTAGAATGGAAGACAACAAGGCTAAAGACTCTGCAGAGGCTGAACAGCAAAACAGCTCAGAAAACCACTCGAAGTCACCTGAATCATCAACGTTGCAGACAGAAGACAACAGATCAGAAAACTTTGCGGATGCTGGACCTCAAAACCGCTCAAGGTCACCAGAATCATCAATGTCACGGACAGGACAAAAGGAAGTCATTGAACAATTTGAACCGGGCGTTTATGTAACTCTCCTTCAACTAAAAAATGGTATTTGGGTTTTTAAGCGTGTTAAATTCAGCAAACGGAAGTTTAGTTCGGAACAGGCAGAAGCATGGTGGAAAAACAACAAAGATAGGCTGCTTAGGAGATATAGTCAGCCGAATACAAGTCCTGCTCCGAGTGTACCACCACCCAGCCGTCCTATACCGCCCGAGGAAAACAGCGAGGCAGCCGCAGCTTCCCCGACTTAG

Protein Analysis

1078

Amino Acids

117.93

Weight (kDa)

8.66

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 15 - 121 2.3e-06 Pleckstrin homology domain
WD40_RLD PF25390 225 - 600 1.3e-77 RCC1-like domain
RCC1 PF00415 281 - 330 1.4e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 320 - 344 1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 333 - 384 1.1e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 388 - 437 5.9e-15 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 453 - 499 2.9e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 502 - 551 1.8e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 556 - 600 8.7e-15 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 607 - 673 2.9e-15 FYVE zinc finger
BRX_N PF13713 857 - 889 1.4e-08 Transcription factor BRX N-terminal domain
BRX PF08381 991 - 1045 1.1e-21 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1902
AasI GACNNNNNNGTC 2 cut(s) 1047, 2317
AccBSI CCGCTC 1 cut(s) 2931
AccI GTMKAC 3 cut(s) 391, 1861, 2378
AciI CCGC 8 cut(s) 362, 1287, 1527, 1785, 2909, 2929, 3191, 3216
AclI AACGTT 2 cut(s) 2292, 2873
AclWI GGATC 5 cut(s) 2, 1224, 1599, 1832, 2190
AcoI YGGCCR 2 cut(s) 445, 1540
AcsI RAATTY 5 cut(s) 126, 526, 727, 953, 3056
AcuI CTGAAG 3 cut(s) 357, 1094, 2501
AfaI GTAC 8 cut(s) 974, 1027, 1248, 1336, 1575, 1587, 2683, 3168
AfiI CCNNNNNNNGG 5 cut(s) 256, 361, 495, 1103, 1973
AflII CTTAAG 3 cut(s) 356, 2015, 2417
AflIII ACRYGT 3 cut(s) 820, 980, 2530
AgeI ACCGGT 1 cut(s) 1091
AhlI ACTAGT 1 cut(s) 1912
AjiI CACGTC 1 cut(s) 477
AjnI CCWGG 5 cut(s) 857, 1364, 1972, 2182, 2466
AleI CACNNNNGTG 1 cut(s) 981
AloI GAACNNNNNNTCC 4 cut(s) 1098, 1130, 3061, 3093
Alw21I GWGCWC 1 cut(s) 2284
Alw26I GTCTC 5 cut(s) 202, 897, 1438, 2325, 2353
Alw44I GTGCAC 1 cut(s) 2280
AlwI GGATC 5 cut(s) 2, 1224, 1599, 1832, 2190
AlwNI CAGNNNCTG 2 cut(s) 11, 2822
Ama87I CYCGRG 1 cut(s) 3194
AoxI GGCC 7 cut(s) 352, 445, 1207, 1324, 1540, 1962, 2720
ApaLI GTGCAC 1 cut(s) 2280
ApeKI GCWGC 7 cut(s) 1304, 1955, 2021, 2582, 3121, 3212, 3218
ApoI RAATTY 5 cut(s) 126, 526, 727, 953, 3056
ArsI GACNNNNNNTTYG 2 cut(s) 2580, 2612
AseI ATTAAT 1 cut(s) 942
AsiGI ACCGGT 1 cut(s) 1091
Asp700I GAANNNNTTC 2 cut(s) 438, 1107
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 6 cut(s) 710, 1094, 1207, 1324, 1963, 2918
AsuC2I CCSGG 3 cut(s) 213, 2374, 2997
AsuII TTCGAA 2 cut(s) 598, 1160
AsuNHI GCTAGC 2 cut(s) 14, 1465
AvaI CYCGRG 1 cut(s) 3194
AvaII GGWCC 3 cut(s) 710, 1094, 2918
BaeGI GKGCMC 1 cut(s) 2284
BaeI ACNNNNGTAYC 2 cut(s) 1238, 1271
BalI TGGCCA 2 cut(s) 447, 1542
BarI GAAGNNNNNNTAC 6 cut(s) 1327, 1359, 2622, 2654, 3000, 3032
BbsI GAAGAC 7 cut(s) 886, 1705, 1728, 2637, 2772, 2799, 2892
Bbv12I GWGCWC 1 cut(s) 2284
BbvCI CCTCAGC 3 cut(s) 330, 2025, 2691
BbvI GCAGC 7 cut(s) 1316, 1967, 2008, 2569, 3108, 3224, 3230
BceAI ACGGC 2 cut(s) 2302, 3165
BciT130I CCWGG 5 cut(s) 859, 1366, 1974, 2184, 2468
BciVI GTATCC 3 cut(s) 511, 1068, 1245
BclI TGATCA 1 cut(s) 1837
BcnI CCSGG 3 cut(s) 213, 2374, 2997
BcoDI GTCTC 5 cut(s) 202, 897, 1438, 2325, 2353
BcuI ACTAGT 1 cut(s) 1912
BfmI CTRYAG 4 cut(s) 1302, 2142, 2211, 2814
BfrI CTTAAG 3 cut(s) 356, 2015, 2417
BfuI GTATCC 3 cut(s) 511, 1068, 1245
BglI GCCNNNNNGGC 1 cut(s) 1548
BglII AGATCT 4 cut(s) 690, 2057, 2336, 2731
BisI GCNGC 9 cut(s) 1305, 1786, 1956, 2022, 2583, 3122, 3213, 3216, 3219
BlsI GCNGC 9 cut(s) 1306, 1787, 1957, 2023, 2584, 3123, 3214, 3217, 3220
Bme1390I CCNGG 8 cut(s) 213, 859, 1366, 1974, 2184, 2374, 2468, 2997
Bme18I GGWCC 3 cut(s) 710, 1094, 2918
BmeT110I CYCGRG 1 cut(s) 3194
BmgBI CACGTC 1 cut(s) 477
BmgT120I GGNCC 6 cut(s) 710, 1094, 1207, 1324, 1963, 2918
BmiI GGNNCC 2 cut(s) 73, 1965
BmrFI CCNGG 8 cut(s) 213, 859, 1366, 1974, 2184, 2374, 2468, 2997
BmrI ACTGGG 2 cut(s) 70, 1712
BmsI GCATC 5 cut(s) 1821, 1987, 2448, 2485, 2902
BmtI GCTAGC 2 cut(s) 18, 1469
BmuI ACTGGG 2 cut(s) 70, 1712
BoxI GACNNNNGTC 1 cut(s) 909
BpiI GAAGAC 7 cut(s) 886, 1705, 1728, 2637, 2772, 2799, 2892
BpmI CTGGAG 1 cut(s) 1851
Bpu10I CCTNAGC 5 cut(s) 330, 1852, 2025, 2691, 3125
Bpu14I TTCGAA 2 cut(s) 598, 1160
BpuEI CTTGAG 4 cut(s) 2183, 2427, 2484, 2917
BpuMI CCSGG 3 cut(s) 213, 2374, 2997
Bsa29I ATCGAT 2 cut(s) 747, 1594
BsaAI YACGTR 2 cut(s) 1014, 2525
BsaBI GATNNNNATC 1 cut(s) 2200
BsaI GGTCTC 1 cut(s) 2353
BsaWI WCCGGW 1 cut(s) 1091
BsaXI ACNNNNNCTCC 6 cut(s) 1235, 1265, 2350, 2380, 3121, 3151
Bsc4I CCNNNNNNNGG 5 cut(s) 256, 361, 495, 1103, 1973
Bse118I RCCGGY 1 cut(s) 1091
Bse1I ACTGG 6 cut(s) 76, 489, 1076, 1327, 1707, 2043
Bse3DI GCAATG 4 cut(s) 54, 483, 1918, 2675
Bse8I GATNNNNATC 1 cut(s) 2200
BseBI CCWGG 5 cut(s) 859, 1366, 1974, 2184, 2468
BseCI ATCGAT 2 cut(s) 747, 1594
BseGI GGATG 4 cut(s) 448, 1812, 2234, 2917
BseJI GATNNNNATC 1 cut(s) 2200
BseLI CCNNNNNNNGG 5 cut(s) 256, 361, 495, 1103, 1973
BseMI GCAATG 4 cut(s) 54, 483, 1918, 2675
BseNI ACTGG 6 cut(s) 76, 489, 1076, 1327, 1707, 2043
BseSI GKGCMC 1 cut(s) 2284
BseXI GCAGC 7 cut(s) 1316, 1967, 2008, 2569, 3108, 3224, 3230
BseYI CCCAGC 1 cut(s) 3176
BsgI GTGCAG 2 cut(s) 603, 2596
BshFI GGCC 7 cut(s) 354, 447, 1209, 1326, 1542, 1964, 2722
BshTI ACCGGT 1 cut(s) 1091
BshVI ATCGAT 2 cut(s) 747, 1594
BsiHKAI GWGCWC 1 cut(s) 2284
BsiHKCI CYCGRG 1 cut(s) 3194
BsiSI CCGG 5 cut(s) 94, 213, 1092, 2374, 2996
BsiWI CGTACG 1 cut(s) 2681
BslFI GGGAC 2 cut(s) 1719, 1727
BslI CCNNNNNNNGG 5 cut(s) 256, 361, 495, 1103, 1973
BsmAI GTCTC 5 cut(s) 202, 897, 1438, 2325, 2353
BsmBI CGTCTC 1 cut(s) 2325
BsmFI GGGAC 2 cut(s) 1719, 1727
BsmI GAATGC 1 cut(s) 1141
BsnI GGCC 7 cut(s) 354, 447, 1209, 1326, 1542, 1964, 2722
Bso31I GGTCTC 1 cut(s) 2353
BsoBI CYCGRG 1 cut(s) 3194
Bsp119I TTCGAA 2 cut(s) 598, 1160
Bsp1286I GDGCHC 1 cut(s) 2284
Bsp19I CCATGG 2 cut(s) 1210, 1521
BspACI CCGC 8 cut(s) 362, 1287, 1527, 1785, 2909, 2929, 3191, 3216
BspANI GGCC 7 cut(s) 354, 447, 1209, 1326, 1542, 1964, 2722
BspDI ATCGAT 2 cut(s) 747, 1594
BspHI TCATGA 2 cut(s) 514, 2307
BspLI GGNNCC 2 cut(s) 73, 1965
BspMAI CTGCAG 2 cut(s) 1306, 2818
BspOI GCTAGC 2 cut(s) 18, 1469
BspPI GGATC 5 cut(s) 2, 1224, 1599, 1832, 2190
BspT104I TTCGAA 2 cut(s) 598, 1160
BspTI CTTAAG 3 cut(s) 356, 2015, 2417
BspTNI GGTCTC 1 cut(s) 2353
BsrBI CCGCTC 1 cut(s) 2931
BsrDI GCAATG 4 cut(s) 54, 483, 1918, 2675
BsrFI RCCGGY 1 cut(s) 1091
BsrI ACTGG 6 cut(s) 76, 489, 1076, 1327, 1707, 2043
BssAI RCCGGY 1 cut(s) 1091
BssT1I CCWWGG 5 cut(s) 768, 1210, 1238, 1521, 2766
Bst2UI CCWGG 5 cut(s) 859, 1366, 1974, 2184, 2468
Bst4CI ACNGT 5 cut(s) 293, 1251, 1898, 1907, 2414
Bst6I CTCTTC 2 cut(s) 282, 1441
BstAFI CTTAAG 3 cut(s) 356, 2015, 2417
BstAPI GCANNNNNTGC 3 cut(s) 11, 56, 1782
BstBAI YACGTR 2 cut(s) 1014, 2525
BstBI TTCGAA 2 cut(s) 598, 1160
BstC8I GCNNGC 8 cut(s) 16, 352, 535, 1285, 1467, 1544, 2545, 2688
BstDSI CCRYGG 2 cut(s) 1210, 1521
BstEII GGTNACC 2 cut(s) 1087, 2937
BstENI CCTNNNNNAGG 1 cut(s) 1101
BstF5I GGATG 4 cut(s) 448, 1812, 2234, 2917
BstHHI GCGC 1 cut(s) 249
BstMAI GTCTC 5 cut(s) 202, 897, 1438, 2325, 2353
BstNI CCWGG 5 cut(s) 859, 1366, 1974, 2184, 2468
BstNSI RCATGY 9 cut(s) 568, 824, 966, 984, 1131, 1287, 1473, 1769, 2534
BstPAI GACNNNNGTC 1 cut(s) 909
BstPI GGTNACC 2 cut(s) 1087, 2937
BstSCI CCNGG 8 cut(s) 211, 857, 1364, 1972, 2182, 2372, 2466, 2995
BstSFI CTRYAG 4 cut(s) 1302, 2142, 2211, 2814
BstSLI GKGCMC 1 cut(s) 2284
BstSNI TACGTA 1 cut(s) 2525
BstV1I GCAGC 7 cut(s) 1316, 1967, 2008, 2569, 3108, 3224, 3230
BstV2I GAAGAC 7 cut(s) 886, 1705, 1728, 2637, 2772, 2799, 2892
BstX2I RGATCY 7 cut(s) 7, 690, 1824, 2057, 2195, 2336, 2731
BstXI CCANNNNNNTGG 1 cut(s) 455
BstYI RGATCY 7 cut(s) 7, 690, 1824, 2057, 2195, 2336, 2731
Bsu15I ATCGAT 2 cut(s) 747, 1594
BsuI GTATCC 3 cut(s) 511, 1068, 1245
BsuRI GGCC 7 cut(s) 354, 447, 1209, 1326, 1542, 1964, 2722
BsuTUI ATCGAT 2 cut(s) 747, 1594
BtgI CCRYGG 2 cut(s) 1210, 1521
BtgZI GCGATG 1 cut(s) 1386
BtrI CACGTC 1 cut(s) 477
BtsCI GGATG 4 cut(s) 448, 1812, 2234, 2917
BtsIMutI CAGTG 5 cut(s) 496, 528, 1069, 1946, 2050
Cac8I GCNNGC 8 cut(s) 16, 352, 535, 1285, 1467, 1544, 2545, 2688
CaiI CAGNNNCTG 2 cut(s) 11, 2822
CciI TCATGA 2 cut(s) 514, 2307
CfoI GCGC 1 cut(s) 249
Cfr10I RCCGGY 1 cut(s) 1091
Cfr13I GGNCC 6 cut(s) 710, 1094, 1207, 1324, 1963, 2918
ClaI ATCGAT 2 cut(s) 747, 1594
CsiI ACCWGGT 1 cut(s) 2466
Csp6I GTAC 8 cut(s) 973, 1026, 1247, 1335, 1574, 1586, 2682, 3167
CspAI ACCGGT 1 cut(s) 1091
CviQI GTAC 8 cut(s) 973, 1026, 1247, 1335, 1574, 1586, 2682, 3167
DrdI GACNNNNNNGTC 2 cut(s) 1047, 2317
DseDI GACNNNNNNGTC 2 cut(s) 1047, 2317
EaeI YGGCCR 2 cut(s) 445, 1540
Eam1104I CTCTTC 2 cut(s) 282, 1441
EarI CTCTTC 2 cut(s) 282, 1441
EciI GGCGGA 1 cut(s) 1542
Eco105I TACGTA 1 cut(s) 2525
Eco130I CCWWGG 5 cut(s) 768, 1210, 1238, 1521, 2766
Eco31I GGTCTC 1 cut(s) 2353
Eco47I GGWCC 3 cut(s) 710, 1094, 2918
Eco57I CTGAAG 3 cut(s) 357, 1094, 2501
Eco88I CYCGRG 1 cut(s) 3194
Eco91I GGTNACC 2 cut(s) 1087, 2937
EcoNI CCTNNNNNAGG 1 cut(s) 1101
EcoO65I GGTNACC 2 cut(s) 1087, 2937
EcoRII CCWGG 5 cut(s) 857, 1364, 1972, 2182, 2466
EcoT14I CCWWGG 5 cut(s) 768, 1210, 1238, 1521, 2766
EcoT22I ATGCAT 1 cut(s) 1814
ErhI CCWWGG 5 cut(s) 768, 1210, 1238, 1521, 2766
Esp3I CGTCTC 1 cut(s) 2325
FalI AAGNNNNNCTT 2 cut(s) 248, 280
FaqI GGGAC 2 cut(s) 1719, 1727
FauNDI CATATG 1 cut(s) 1537
FbaI TGATCA 1 cut(s) 1837
FblI GTMKAC 3 cut(s) 391, 1861, 2378
Fnu4HI GCNGC 9 cut(s) 1305, 1786, 1956, 2022, 2583, 3122, 3213, 3216, 3219
FokI GGATG 4 cut(s) 435, 1799, 2221, 2924
Fsp4HI GCNGC 9 cut(s) 1305, 1786, 1956, 2022, 2583, 3122, 3213, 3216, 3219
GlaI GCGC 1 cut(s) 248
GluI GCNGC 9 cut(s) 1305, 1786, 1956, 2022, 2583, 3122, 3213, 3216, 3219
GsaI CCCAGC 1 cut(s) 3180
GsuI CTGGAG 1 cut(s) 1851
HaeIII GGCC 7 cut(s) 354, 447, 1209, 1326, 1542, 1964, 2722
HapII CCGG 5 cut(s) 94, 213, 1092, 2374, 2996
HhaI GCGC 1 cut(s) 249
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HincII GTYRAC 1 cut(s) 1862
HindII GTYRAC 1 cut(s) 1862
HpaII CCGG 5 cut(s) 94, 213, 1092, 2374, 2996
HpyAV CCTTC 5 cut(s) 231, 1097, 2217, 2712, 3026
HpyCH4III ACNGT 5 cut(s) 293, 1251, 1898, 1907, 2414
HpyCH4IV ACGT 7 cut(s) 394, 476, 556, 1013, 2292, 2524, 2873
HpySE526I ACGT 7 cut(s) 394, 476, 556, 1013, 2292, 2524, 2873
HspAI GCGC 1 cut(s) 247
Ksp22I TGATCA 1 cut(s) 1837
LmnI GCTCC 3 cut(s) 1190, 1832, 3163
Lsp1109I GCAGC 7 cut(s) 1316, 1967, 2008, 2569, 3108, 3224, 3230
LweI GCATC 5 cut(s) 1821, 1987, 2448, 2485, 2902
MabI ACCWGGT 1 cut(s) 2466
MaeII ACGT 7 cut(s) 394, 476, 556, 1013, 2292, 2524, 2873
MaeIII GTNAC 9 cut(s) 832, 1087, 1376, 1394, 2065, 2856, 2937, 2955, 3007
MbiI CCGCTC 1 cut(s) 2931
MflI RGATCY 7 cut(s) 7, 690, 1824, 2057, 2195, 2336, 2731
MhlI GDGCHC 1 cut(s) 2284
MlsI TGGCCA 2 cut(s) 447, 1542
MluNI TGGCCA 2 cut(s) 447, 1542
MlyI GAGTC 7 cut(s) 419, 782, 876, 1963, 2434, 2786, 2804
MmeI TCCRAC 1 cut(s) 1760
Mox20I TGGCCA 2 cut(s) 447, 1542
Mph1103I ATGCAT 1 cut(s) 1814
MroXI GAANNNNTTC 2 cut(s) 438, 1107
MscI TGGCCA 2 cut(s) 447, 1542
MslI CAYNNNNRTG 3 cut(s) 981, 1923, 2490
Msp20I TGGCCA 2 cut(s) 447, 1542
MspA1I CMGCKG 1 cut(s) 227
MspCI CTTAAG 3 cut(s) 356, 2015, 2417
MspI CCGG 5 cut(s) 94, 213, 1092, 2374, 2996
MspR9I CCNGG 8 cut(s) 213, 859, 1366, 1974, 2184, 2374, 2468, 2997
Mva1269I GAATGC 1 cut(s) 1141
MvaI CCWGG 5 cut(s) 859, 1366, 1974, 2184, 2468
NciI CCSGG 3 cut(s) 213, 2374, 2997
NcoI CCATGG 2 cut(s) 1210, 1521
NdeI CATATG 1 cut(s) 1537
NheI GCTAGC 2 cut(s) 14, 1465
NlaIV GGNNCC 2 cut(s) 73, 1965
NmuCI GTSAC 6 cut(s) 1376, 1394, 2065, 2856, 2937, 2955
NsiI ATGCAT 1 cut(s) 1814
NspI RCATGY 9 cut(s) 568, 824, 966, 984, 1131, 1287, 1473, 1769, 2534
NspV TTCGAA 2 cut(s) 598, 1160
OliI CACNNNNGTG 1 cut(s) 981
PaeI GCATGC 1 cut(s) 1287
PagI TCATGA 2 cut(s) 514, 2307
PciI ACATGT 3 cut(s) 820, 980, 2530
PctI GAATGC 1 cut(s) 1141
PdmI GAANNNNTTC 2 cut(s) 438, 1107
Pfl23II CGTACG 1 cut(s) 2681
PflFI GACNNNGTC 3 cut(s) 396, 885, 2364
PfoI TCCNGGA 1 cut(s) 1972
PinAI ACCGGT 1 cut(s) 1091
PkrI GCNGC 9 cut(s) 1306, 1787, 1957, 2023, 2584, 3123, 3214, 3217, 3220
PleI GAGTC 7 cut(s) 419, 781, 876, 1963, 2434, 2785, 2804
PpsI GAGTC 7 cut(s) 419, 781, 876, 1963, 2434, 2785, 2804
Ppu21I YACGTR 2 cut(s) 1014, 2525
PscI ACATGT 3 cut(s) 820, 980, 2530
PshAI GACNNNNGTC 1 cut(s) 909
PshBI ATTAAT 1 cut(s) 942
PsiI TTATAA 1 cut(s) 1902
Psp1406I AACGTT 2 cut(s) 2292, 2873
Psp6I CCWGG 5 cut(s) 857, 1364, 1972, 2182, 2466
PspEI GGTNACC 2 cut(s) 1087, 2937
PspFI CCCAGC 1 cut(s) 3176
PspGI CCWGG 5 cut(s) 857, 1364, 1972, 2182, 2466
PspLI CGTACG 1 cut(s) 2681
PspN4I GGNNCC 2 cut(s) 73, 1965
PspPI GGNCC 6 cut(s) 710, 1094, 1207, 1324, 1963, 2918
PstI CTGCAG 2 cut(s) 1306, 2818
PstNI CAGNNNCTG 2 cut(s) 11, 2822
PsuI RGATCY 7 cut(s) 7, 690, 1824, 2057, 2195, 2336, 2731
PsyI GACNNNGTC 3 cut(s) 396, 885, 2364
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 8 cut(s) 974, 1027, 1248, 1336, 1575, 1587, 2683, 3168
RsaNI GTAC 8 cut(s) 973, 1026, 1247, 1335, 1574, 1586, 2682, 3167
RseI CAYNNNNRTG 3 cut(s) 981, 1923, 2490
SalI GTCGAC 1 cut(s) 1860
SatI GCNGC 9 cut(s) 1305, 1786, 1956, 2022, 2583, 3122, 3213, 3216, 3219
Sau96I GGNCC 6 cut(s) 710, 1094, 1207, 1324, 1963, 2918
SchI GAGTC 7 cut(s) 419, 782, 876, 1963, 2434, 2786, 2804
ScrFI CCNGG 8 cut(s) 213, 859, 1366, 1974, 2184, 2374, 2468, 2997
SduI GDGCHC 1 cut(s) 2284
SexAI ACCWGGT 1 cut(s) 2466
SfaNI GCATC 5 cut(s) 1821, 1987, 2448, 2485, 2902
SfcI CTRYAG 4 cut(s) 1302, 2142, 2211, 2814
SfuI TTCGAA 2 cut(s) 598, 1160
SinI GGWCC 3 cut(s) 710, 1094, 2918
SmiMI CAYNNNNRTG 3 cut(s) 981, 1923, 2490
SmlI CTYRAG 7 cut(s) 356, 2015, 2162, 2417, 2442, 2499, 2932
SmoI CTYRAG 7 cut(s) 356, 2015, 2162, 2417, 2442, 2499, 2932
SnaBI TACGTA 1 cut(s) 2525
SpeI ACTAGT 1 cut(s) 1912
SphI GCATGC 1 cut(s) 1287
SsiI CCGC 8 cut(s) 362, 1287, 1527, 1785, 2909, 2929, 3191, 3216
SspI AATATT 1 cut(s) 2713
StyD4I CCNGG 8 cut(s) 211, 857, 1364, 1972, 2182, 2372, 2466, 2995
StyI CCWWGG 5 cut(s) 768, 1210, 1238, 1521, 2766
TaaI ACNGT 5 cut(s) 293, 1251, 1898, 1907, 2414
TaiI ACGT 7 cut(s) 397, 479, 559, 1016, 2295, 2527, 2876
TaqII GACCGA 1 cut(s) 875
TatI WGTACW 1 cut(s) 1585
TauI GCSGC 2 cut(s) 1788, 3218
TscAI CASTG 5 cut(s) 496, 528, 1076, 1953, 2050
TseFI GTSAC 6 cut(s) 1376, 1394, 2065, 2856, 2937, 2955
TseI GCWGC 7 cut(s) 1304, 1955, 2021, 2582, 3121, 3212, 3218
Tsp45I GTSAC 6 cut(s) 1376, 1394, 2065, 2856, 2937, 2955
TspDTI ATGAA 7 cut(s) 104, 153, 431, 531, 1335, 2192, 2717
TspGWI ACGGA 5 cut(s) 493, 1221, 2624, 2974, 3082
TspRI CASTG 5 cut(s) 496, 528, 1076, 1953, 2050
Tth111I GACNNNGTC 3 cut(s) 396, 885, 2364
Vha464I CTTAAG 3 cut(s) 356, 2015, 2417
VneI GTGCAC 1 cut(s) 2280
VpaK11BI GGWCC 3 cut(s) 710, 1094, 2918
VspI ATTAAT 1 cut(s) 942
XagI CCTNNNNNAGG 1 cut(s) 1101
XapI RAATTY 5 cut(s) 126, 526, 727, 953, 3056
XbaI TCTAGA 1 cut(s) 2783
XceI RCATGY 9 cut(s) 568, 824, 966, 984, 1131, 1287, 1473, 1769, 2534
XcmI CCANNNNNNNNNTGG 1 cut(s) 1469
XmiI GTMKAC 3 cut(s) 391, 1861, 2378
XmnI GAANNNNTTC 2 cut(s) 438, 1107
Zsp2I ATGCAT 1 cut(s) 1814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.