MD16G1219800.v1.1

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006555
Physical Location & Seq
Forward (+)
21826402 .. 21831900
5499 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1219800.v1.1.491

Sequence Viewer

Length: 3204 bp
ATGAAGGCACTCACTGCATTAAAAAAAGGTTCCCAGTTAATCAAGTATAGCCGGAAAGGGAAGCCAAAACTTTGTTCATTCAGAATTTCTACTGATGAAACTACACTAATCTGGTATTCACATGGAGAAGAACGAACTGTGAAATTAGCTTCTGTCTCTCGAATTATCCCTGGACAGAGAACGGCTGTATTTAGAAGGTATTTGCGCCCAGAAAAGGATTACTTGTCATTTTCTCTTCTATATAACAACGGTGAACGAACACTTGATCTGATCTGCAAGGACAAAGCTGAGGCAGAGGTATGGTTTGCTGGCCTTAAGGCACTAATTACTTCAGGACAACAACGTAGTAGACGTACTAAGAGTGATATTTATGATCTGCAAGACTGTGCTGAATCTGTTAATGGTCGTCCTTTTGGTGCGGCCTTAGATTTCACTTCGAGCATTGCCCGTGGTAGGGGATCTGTTGATTCTCGTGAATCAGTGAATTTTTCAGGCTCAGATGTGGGTTCAGAACGTGCAAATATGCAACTAAGAACAAGTGCAGGAGATGGTTTTCGAATTAGTGTTTCAAGCACTCCTAGCTGTTCAAGTGGAGGGTCTGGACCTGATGACATTGAATCACTAGGAGATGTTTATGTTTGGGGAGAGATCTGGTCTGATGGAAATGGACCTGATGGGTCCACGAATTCAATCCCTACAAAAACCAATGTGCTGATTCCAAAGCCCTTGGAGTCGAATGTTGTTCTTGATGTTCAGCAGATAGCTTGTGGTGTGCGACATGTTGCTCTTGTAACAAGGCAAGGGGAGGTTTTTACCTGGGGAGAGGAATCTGGGGGAAGACTTGGTCATGGGATTGATAGAGACTTTAGTTGTCCTCGCCTTGTTGAGTTCCTCGCAATTAATAACATTGAATTTGTTGCATGTGGTGAGTACCACACATGTGCTGTATCTACGTCAGGTGATTTGTTTACGTGGGGTGATGGTACACATAATGCTGGACTTCTTGGTCTTGGAACAGATGTTAGCCACTGGATACCTAAAAGGGTTACCGGTCCTTTAGAAGGACTTCAGGTTCTATCTGTTGCGTGTGGCACGTGGCATTCAGCTTTGGCAACTTCAAATGGAAAGCTGTTCACGTTTGGAGATGGAGCGTTTGGTGTTTTGGGCCATGGAGATCGAGAGAGTGTTCTGTATCCAAGGGATGTACAGTTGTTGAATGGACTAAAGACGATAAAAGTAGCATGCGGAGTTTGGCATACTGCAGCAATTGTAGAGGTTATGGGCCAGTCTGGTGCAAATGCTTCATCTCGTAAGTTGTTCACCTGGGGTGATGGTGACAAACATTGTTTGGGTCACGGAAGCAAGGAAACTTATCTGCTTCCCACCTGTGTCTCTTCACTTATTGACTATAATTTCCACCAGCTAGCATGTGGACACACTATGACTATTGCCCTCACCACATCAGGTCATGTGTTTACCATGGGCGGAACTGCTTATGGTCAGCTAGGCAATCCAACTTCTGACGGGAAAGTACCTTGCTTAGTACAGGATCGATTGATTGGTGAGTTTGTCGAAGAAATATCTTGTGGGTCATATCATGTTGCTGTCCTGACATCAAGAAGTGAAGTGTTCACTTGGGGAAGAGGGGCTAATGGAAGACTGGGACACGGGGACACAGAAGACAGGAAAACTCCAACTTTGGTTGAAGCCCTGAAAGATAGGCATGTGAAAAGCATATCGTGTGGCTCAAACTTCACATCTAGTATATGCATCCATAAGTGGGTCTCTGGAGCTGATCAATCAATTTGTTCAGGTTGTCGACAGACATTTGGTTTTACTAGAAAGAGGCATAACTGTTATAACTGTGGACTAGTCCATTGCCATGCTTGTAGTTCCAAAAAAGCACTGAGGGCAGCATTGGCCCCGACTCCTGGAAAACCACACCGTGTATGTGATGCTTGTTATGCAAAACTTAAAGCTGCTGAGGCTGGTAATGCTTCCAATGTTAGTAGGAGATCTATGATCACACGCTCAATGGATAGCAGGGATTTTCTAAATAGGGGAGAGGTGAAATCTTCAAGGATTCTTCTCTCTTCCACTACAGAACCCGTTAAATACCTTGAGATCAAGTCCATGAAGCCTGGGGGTGTCAGATCCGAGTCCCCTTCTATAGTCAGGGCTTCCCAAGTTCCATCCCTTTTACCACTAAAAGATATTGCGTTTCCAAGTTCATTGAGTGCACTTCAAAACGCTTTGAAGCCTGTCATGACAATGCTGCCTCAGCCCAATTCAAGATCTACTTCACCATATTCGAGGAGACCTAGTCCTCCCCGGTCTACAACTCCTATATTCTCTAGGAGTGTTATTGACAGTCTTAAGAGGACAAATGACACTCTGACTCAAGAAGTGCTGAAGTTGCAAAACCAGGTTAAAAGTCTGAAGCAGAAGTGTGATGCTCAAGATGTAGAGATTCAAAAACTAAATAAGCATGCCAAAGAAGCTGGCTCATTAGCCAATGAACAATACTCTAAGTGCAGAGCTGCCAAAGAACTTGTCAAGTCCATCGCTGAACAGATGAGGGGGTGGGAAGACAAGATACCTCCTGAGATTTTTGACAGTGATACTTTCAAAGCATTGCGTACGCAAGCTAAAGATTTTATGAACATGAATATTGGAAGCTCAAGCTCAGAGCTAGGCCAACACTATGCAGCCGATAGAACTTCCTTGGTTGTTGAGTCTTCTAGAATGGACGACAATAGAGCAGACGACTCTGCAGAGGCTGAACCTCAAAACAGCTCAGAAAACCGCTCAAGGTCACCTGAATCATCAACATTGAGGACAGAAGACAACAGAGCAGAAGACTTTGCGGAGGCTGAACCTCAAAACAGCTCAGAAAACCGCTTGAGGTCACCTGAATCATCAACGTCGCATAGAGGACAAAAGGAAGTCATTGAACAATTTGAACCGGGCGTTTATGTAACTCTCCTTCAACAACAAAATGGTACTAGGATTTTTAGGCGTGTTAAATTCAGCAAACGAAAGTTTAGTTCGGAACAGGCAGAAGCATGGTGGAAAAATAACAAAGATAGGCTGCTTAGGAGATATAGTCAGCCGAGTGCAAGTCCTGCTCCGAGTGTACCACCACCCAGCAGTCCTGTACCGCCGGAGGAAAACAGCGAGGCAGCCGCAGCTTCCCCGACTTAG

Protein Analysis

1068

Amino Acids

116.56

Weight (kDa)

8.79

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 6 - 109 8.7e-07 Pleckstrin homology domain
WD40_RLD PF25390 211 - 586 1.4e-77 RCC1-like domain
RCC1 PF00415 267 - 316 4.6e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 306 - 330 1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 319 - 370 1.1e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 374 - 423 3.8e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 439 - 485 4.4e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 488 - 537 3.1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 525 - 552 7.4e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 542 - 586 9.3e-15 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 593 - 659 3.1e-15 FYVE zinc finger
BRX_N PF13713 843 - 875 7.1e-07 Transcription factor BRX N-terminal domain
BRX PF08381 981 - 1035 2.3e-23 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1860
AasI GACNNNNNNGTC 1 cut(s) 1005
AccBSI CCGCTC 1 cut(s) 2808
AccI GTMKAC 3 cut(s) 349, 1819, 2336
AciI CCGC 8 cut(s) 419, 1245, 1485, 2806, 2867, 2899, 3161, 3186
AclWI GGATC 3 cut(s) 466, 1557, 2148
AcsI RAATTY 5 cut(s) 84, 484, 685, 911, 3026
AcuI CTGAAG 4 cut(s) 315, 1052, 2432, 2459
AcvI CACGTG 1 cut(s) 1095
AdeI CACNNNGTG 1 cut(s) 1946
AfiI CCNNNNNNNGG 6 cut(s) 214, 453, 454, 1061, 1780, 1931
AflII CTTAAG 2 cut(s) 314, 2375
AflIII ACRYGT 2 cut(s) 778, 938
AgeI ACCGGT 1 cut(s) 1049
AhlI ACTAGT 1 cut(s) 1870
AjnI CCWGG 6 cut(s) 169, 815, 1322, 1930, 2140, 2424
AjuI GAANNNNNNNTTGG 2 cut(s) 58, 90
AleI CACNNNNGTG 1 cut(s) 939
AloI GAACNNNNNNTCC 2 cut(s) 1056, 1088
Alw21I GWGCWC 1 cut(s) 2242
Alw26I GTCTC 5 cut(s) 160, 855, 1396, 1789, 2311
Alw44I GTGCAC 1 cut(s) 2238
AlwI GGATC 3 cut(s) 466, 1557, 2148
AlwNI CAGNNNCTG 1 cut(s) 2780
AoxI GGCC 6 cut(s) 310, 420, 1165, 1282, 1920, 2695
ApaLI GTGCAC 1 cut(s) 2238
ApeKI GCWGC 9 cut(s) 1262, 1913, 1979, 2275, 2540, 2708, 3091, 3182, 3188
ApoI RAATTY 5 cut(s) 84, 484, 685, 911, 3026
ArsI GACNNNNNNTTYG 2 cut(s) 2538, 2570
AseI ATTAAT 1 cut(s) 900
AsiGI ACCGGT 1 cut(s) 1049
Asp700I GAANNNNTTC 1 cut(s) 1065
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 7 cut(s) 602, 668, 678, 1052, 1165, 1282, 1921
AsuC2I CCSGG 2 cut(s) 2332, 2967
AsuII TTCGAA 1 cut(s) 556
AsuNHI GCTAGC 1 cut(s) 1423
AvaII GGWCC 4 cut(s) 602, 668, 678, 1052
BaeGI GKGCMC 1 cut(s) 2242
BarI GAAGNNNNNNTAC 4 cut(s) 2580, 2612, 2970, 3002
BauI CACGAG 1 cut(s) 471
BbrPI CACGTG 1 cut(s) 1095
BbsI GAAGAC 7 cut(s) 844, 1663, 1686, 2595, 2730, 2850, 2865
Bbv12I GWGCWC 1 cut(s) 2242
BbvCI CCTCAGC 3 cut(s) 288, 1983, 2280
BbvI GCAGC 9 cut(s) 1274, 1925, 1966, 2262, 2527, 2720, 3078, 3194, 3200
BccI CCATC 8 cut(s) 542, 653, 668, 974, 1139, 1325, 2200, 2570
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 6 cut(s) 171, 817, 1324, 1932, 2142, 2426
BciVI GTATCC 2 cut(s) 1026, 1203
BclI TGATCA 2 cut(s) 1795, 2022
BcnI CCSGG 2 cut(s) 2332, 2967
BcoDI GTCTC 5 cut(s) 160, 855, 1396, 1789, 2311
BcuI ACTAGT 1 cut(s) 1870
BfmI CTRYAG 4 cut(s) 1260, 2100, 2169, 2772
BfrI CTTAAG 2 cut(s) 314, 2375
BfuI GTATCC 2 cut(s) 1026, 1203
BglII AGATCT 3 cut(s) 648, 2015, 2294
Bme1390I CCNGG 8 cut(s) 171, 817, 1324, 1932, 2142, 2332, 2426, 2967
Bme18I GGWCC 4 cut(s) 602, 668, 678, 1052
BmgT120I GGNCC 7 cut(s) 602, 668, 678, 1052, 1165, 1282, 1921
BmiI GGNNCC 3 cut(s) 31, 679, 1923
BmrFI CCNGG 8 cut(s) 171, 817, 1324, 1932, 2142, 2332, 2426, 2967
BmrI ACTGGG 2 cut(s) 28, 1670
BmsI GCATC 3 cut(s) 1779, 1945, 2443
BmtI GCTAGC 1 cut(s) 1427
BmuI ACTGGG 2 cut(s) 28, 1670
BpiI GAAGAC 7 cut(s) 844, 1663, 1686, 2595, 2730, 2850, 2865
BpmI CTGGAG 1 cut(s) 1809
Bpu10I CCTNAGC 4 cut(s) 288, 1983, 2280, 3095
Bpu14I TTCGAA 1 cut(s) 556
BpuEI CTTGAG 6 cut(s) 2141, 2385, 2442, 2665, 2794, 2923
BpuMI CCSGG 2 cut(s) 2332, 2967
Bsa29I ATCGAT 1 cut(s) 1552
BsaAI YACGTR 2 cut(s) 972, 1095
BsaI GGTCTC 2 cut(s) 1789, 2311
BsaWI WCCGGW 1 cut(s) 1049
BsaXI ACNNNNNCTCC 4 cut(s) 2308, 2338, 3091, 3121
Bsc4I CCNNNNNNNGG 6 cut(s) 214, 453, 454, 1061, 1780, 1931
Bse118I RCCGGY 1 cut(s) 1049
Bse1I ACTGG 4 cut(s) 34, 1034, 1285, 1665
Bse3DI GCAATG 3 cut(s) 441, 1876, 2633
BseBI CCWGG 6 cut(s) 171, 817, 1324, 1932, 2142, 2426
BseCI ATCGAT 1 cut(s) 1552
BseGI GGATG 3 cut(s) 1207, 1770, 2192
BseLI CCNNNNNNNGG 6 cut(s) 214, 453, 454, 1061, 1780, 1931
BseMI GCAATG 3 cut(s) 441, 1876, 2633
BseMII CTCAG 9 cut(s) 279, 510, 1898, 1974, 2294, 2595, 2700, 2811, 2904
BseNI ACTGG 4 cut(s) 34, 1034, 1285, 1665
BseRI GAGGAG 1 cut(s) 2329
BseSI GKGCMC 1 cut(s) 2242
BseXI GCAGC 9 cut(s) 1274, 1925, 1966, 2262, 2527, 2720, 3078, 3194, 3200
BseYI CCCAGC 1 cut(s) 3146
BsgI GTGCAG 2 cut(s) 561, 2554
BshFI GGCC 6 cut(s) 312, 422, 1167, 1284, 1922, 2697
BshTI ACCGGT 1 cut(s) 1049
BshVI ATCGAT 1 cut(s) 1552
BsiHKAI GWGCWC 1 cut(s) 2242
BsiSI CCGG 5 cut(s) 52, 1050, 2332, 2966, 3164
BsiWI CGTACG 1 cut(s) 2639
BslFI GGGAC 3 cut(s) 1677, 1685, 2146
BslI CCNNNNNNNGG 6 cut(s) 214, 453, 454, 1061, 1780, 1931
BsmAI GTCTC 5 cut(s) 160, 855, 1396, 1789, 2311
BsmFI GGGAC 3 cut(s) 1677, 1685, 2146
BsmI GAATGC 1 cut(s) 1099
BsnI GGCC 6 cut(s) 312, 422, 1167, 1284, 1922, 2697
Bso31I GGTCTC 2 cut(s) 1789, 2311
Bsp119I TTCGAA 1 cut(s) 556
Bsp1286I GDGCHC 1 cut(s) 2242
Bsp1407I TGTACA 1 cut(s) 1204
Bsp19I CCATGG 2 cut(s) 1168, 1479
BspACI CCGC 8 cut(s) 419, 1245, 1485, 2806, 2867, 2899, 3161, 3186
BspANI GGCC 6 cut(s) 312, 422, 1167, 1284, 1922, 2697
BspCNI CTCAG 9 cut(s) 280, 509, 1899, 1975, 2293, 2596, 2699, 2810, 2903
BspDI ATCGAT 1 cut(s) 1552
BspHI TCATGA 1 cut(s) 2265
BspLI GGNNCC 3 cut(s) 31, 679, 1923
BspMAI CTGCAG 2 cut(s) 1264, 2776
BspOI GCTAGC 1 cut(s) 1427
BspPI GGATC 3 cut(s) 466, 1557, 2148
BspT104I TTCGAA 1 cut(s) 556
BspTI CTTAAG 2 cut(s) 314, 2375
BspTNI GGTCTC 2 cut(s) 1789, 2311
BsrBI CCGCTC 1 cut(s) 2808
BsrDI GCAATG 3 cut(s) 441, 1876, 2633
BsrFI RCCGGY 1 cut(s) 1049
BsrGI TGTACA 1 cut(s) 1204
BsrI ACTGG 4 cut(s) 34, 1034, 1285, 1665
BssAI RCCGGY 1 cut(s) 1049
BssSI CACGAG 1 cut(s) 471
BssT1I CCWWGG 5 cut(s) 726, 1168, 1196, 1479, 2724
Bst2BI CACGAG 1 cut(s) 471
Bst2UI CCWGG 6 cut(s) 171, 817, 1324, 1932, 2142, 2426
Bst4CI ACNGT 9 cut(s) 139, 251, 386, 1209, 1856, 1865, 1946, 2372, 2618
Bst6I CTCTTC 4 cut(s) 240, 1399, 1636, 2098
BstAFI CTTAAG 2 cut(s) 314, 2375
BstAPI GCANNNNNTGC 2 cut(s) 14, 3125
BstAUI TGTACA 1 cut(s) 1204
BstBAI YACGTR 2 cut(s) 972, 1095
BstBI TTCGAA 1 cut(s) 556
BstC8I GCNNGC 6 cut(s) 310, 1243, 1425, 2490, 2503, 2646
BstDSI CCRYGG 3 cut(s) 448, 1168, 1479
BstEII GGTNACC 3 cut(s) 1045, 2814, 2907
BstENI CCTNNNNNAGG 1 cut(s) 1059
BstF5I GGATG 3 cut(s) 1207, 1770, 2192
BstHHI GCGC 1 cut(s) 207
BstMAI GTCTC 5 cut(s) 160, 855, 1396, 1789, 2311
BstNI CCWGG 6 cut(s) 171, 817, 1324, 1932, 2142, 2426
BstNSI RCATGY 7 cut(s) 782, 924, 942, 1245, 1431, 1727, 2492
BstPI GGTNACC 3 cut(s) 1045, 2814, 2907
BstSCI CCNGG 8 cut(s) 169, 815, 1322, 1930, 2140, 2330, 2424, 2965
BstSFI CTRYAG 4 cut(s) 1260, 2100, 2169, 2772
BstSLI GKGCMC 1 cut(s) 2242
BstV1I GCAGC 9 cut(s) 1274, 1925, 1966, 2262, 2527, 2720, 3078, 3194, 3200
BstV2I GAAGAC 7 cut(s) 844, 1663, 1686, 2595, 2730, 2850, 2865
BstX2I RGATCY 5 cut(s) 458, 648, 2015, 2153, 2294
BstYI RGATCY 5 cut(s) 458, 648, 2015, 2153, 2294
Bsu15I ATCGAT 1 cut(s) 1552
BsuI GTATCC 2 cut(s) 1026, 1203
BsuRI GGCC 6 cut(s) 312, 422, 1167, 1284, 1922, 2697
BsuTUI ATCGAT 1 cut(s) 1552
BtgI CCRYGG 3 cut(s) 448, 1168, 1479
BtgZI GCGATG 1 cut(s) 2548
BtsCI GGATG 3 cut(s) 1207, 1770, 2192
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 5 cut(s) 12, 486, 1027, 1904, 2623
Cac8I GCNNGC 6 cut(s) 310, 1243, 1425, 2490, 2503, 2646
CaiI CAGNNNCTG 1 cut(s) 2780
CciI TCATGA 1 cut(s) 2265
CfoI GCGC 1 cut(s) 207
Cfr10I RCCGGY 1 cut(s) 1049
Cfr13I GGNCC 7 cut(s) 602, 668, 678, 1052, 1165, 1282, 1921
ClaI ATCGAT 1 cut(s) 1552
CsiI ACCWGGT 1 cut(s) 2424
CspAI ACCGGT 1 cut(s) 1049
DraIII CACNNNGTG 1 cut(s) 1946
DrdI GACNNNNNNGTC 1 cut(s) 1005
DseDI GACNNNNNNGTC 1 cut(s) 1005
Eam1104I CTCTTC 4 cut(s) 240, 1399, 1636, 2098
EarI CTCTTC 4 cut(s) 240, 1399, 1636, 2098
EciI GGCGGA 1 cut(s) 1500
Eco130I CCWWGG 5 cut(s) 726, 1168, 1196, 1479, 2724
Eco31I GGTCTC 2 cut(s) 1789, 2311
Eco47I GGWCC 4 cut(s) 602, 668, 678, 1052
Eco57I CTGAAG 4 cut(s) 315, 1052, 2432, 2459
Eco72I CACGTG 1 cut(s) 1095
Eco91I GGTNACC 3 cut(s) 1045, 2814, 2907
EcoNI CCTNNNNNAGG 1 cut(s) 1059
EcoO65I GGTNACC 3 cut(s) 1045, 2814, 2907
EcoRI GAATTC 1 cut(s) 685
EcoRII CCWGG 6 cut(s) 169, 815, 1322, 1930, 2140, 2424
EcoT14I CCWWGG 5 cut(s) 726, 1168, 1196, 1479, 2724
EcoT22I ATGCAT 1 cut(s) 1772
ErhI CCWWGG 5 cut(s) 726, 1168, 1196, 1479, 2724
FalI AAGNNNNNCTT 4 cut(s) 206, 238, 2284, 2316
FaqI GGGAC 3 cut(s) 1677, 1685, 2146
FbaI TGATCA 2 cut(s) 1795, 2022
FblI GTMKAC 3 cut(s) 349, 1819, 2336
FokI GGATG 3 cut(s) 1214, 1757, 2179
GlaI GCGC 1 cut(s) 206
GsaI CCCAGC 1 cut(s) 3150
GsuI CTGGAG 1 cut(s) 1809
HaeIII GGCC 6 cut(s) 312, 422, 1167, 1284, 1922, 2697
HapII CCGG 5 cut(s) 52, 1050, 2332, 2966, 3164
HhaI GCGC 1 cut(s) 207
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HincII GTYRAC 1 cut(s) 1820
HindII GTYRAC 1 cut(s) 1820
HpaII CCGG 5 cut(s) 52, 1050, 2332, 2966, 3164
Hpy99I CGWCG 1 cut(s) 2929
HpyAV CCTTC 4 cut(s) 189, 1055, 2175, 2996
HpyCH4III ACNGT 9 cut(s) 139, 251, 386, 1209, 1856, 1865, 1946, 2372, 2618
HpyCH4IV ACGT 8 cut(s) 343, 352, 514, 953, 971, 1094, 1136, 2924
HpySE526I ACGT 8 cut(s) 343, 352, 514, 953, 971, 1094, 1136, 2924
HspAI GCGC 1 cut(s) 205
Ksp22I TGATCA 2 cut(s) 1795, 2022
LmnI GCTCC 3 cut(s) 1148, 1790, 3133
Lsp1109I GCAGC 9 cut(s) 1274, 1925, 1966, 2262, 2527, 2720, 3078, 3194, 3200
LweI GCATC 3 cut(s) 1779, 1945, 2443
MabI ACCWGGT 1 cut(s) 2424
MaeII ACGT 8 cut(s) 343, 352, 514, 953, 971, 1094, 1136, 2924
MaeIII GTNAC 7 cut(s) 790, 1045, 1334, 1352, 2814, 2907, 2977
MbiI CCGCTC 1 cut(s) 2808
MfeI CAATTG 1 cut(s) 1266
MflI RGATCY 5 cut(s) 458, 648, 2015, 2153, 2294
MhlI GDGCHC 1 cut(s) 2242
MlyI GAGTC 6 cut(s) 740, 1921, 2168, 2392, 2744, 2762
MmeI TCCRAC 2 cut(s) 1538, 1718
Mph1103I ATGCAT 1 cut(s) 1772
MroXI GAANNNNTTC 1 cut(s) 1065
MslI CAYNNNNRTG 3 cut(s) 939, 1881, 2270
MspCI CTTAAG 2 cut(s) 314, 2375
MspI CCGG 5 cut(s) 52, 1050, 2332, 2966, 3164
MspR9I CCNGG 8 cut(s) 171, 817, 1324, 1932, 2142, 2332, 2426, 2967
MunI CAATTG 1 cut(s) 1266
Mva1269I GAATGC 1 cut(s) 1099
MvaI CCWGG 6 cut(s) 171, 817, 1324, 1932, 2142, 2426
NciI CCSGG 2 cut(s) 2332, 2967
NcoI CCATGG 2 cut(s) 1168, 1479
NheI GCTAGC 1 cut(s) 1423
NlaIV GGNNCC 3 cut(s) 31, 679, 1923
NmeAIII GCCGAG 1 cut(s) 3138
NmuCI GTSAC 4 cut(s) 1334, 1352, 2814, 2907
NsiI ATGCAT 1 cut(s) 1772
NspI RCATGY 7 cut(s) 782, 924, 942, 1245, 1431, 1727, 2492
NspV TTCGAA 1 cut(s) 556
OliI CACNNNNGTG 1 cut(s) 939
PaeI GCATGC 2 cut(s) 1245, 2492
PagI TCATGA 1 cut(s) 2265
PciI ACATGT 2 cut(s) 778, 938
PcsI WCGNNNNNNNCGW 1 cut(s) 349
PctI GAATGC 1 cut(s) 1099
PdmI GAANNNNTTC 1 cut(s) 1065
Pfl23II CGTACG 1 cut(s) 2639
PflFI GACNNNGTC 2 cut(s) 843, 2322
PfoI TCCNGGA 1 cut(s) 1930
PinAI ACCGGT 1 cut(s) 1049
PleI GAGTC 6 cut(s) 739, 1921, 2167, 2392, 2743, 2762
PmaCI CACGTG 1 cut(s) 1095
PmlI CACGTG 1 cut(s) 1095
PpsI GAGTC 6 cut(s) 739, 1921, 2167, 2392, 2743, 2762
Ppu21I YACGTR 2 cut(s) 972, 1095
PscI ACATGT 2 cut(s) 778, 938
PshBI ATTAAT 1 cut(s) 900
PsiI TTATAA 1 cut(s) 1860
Psp6I CCWGG 6 cut(s) 169, 815, 1322, 1930, 2140, 2424
PspCI CACGTG 1 cut(s) 1095
PspEI GGTNACC 3 cut(s) 1045, 2814, 2907
PspFI CCCAGC 1 cut(s) 3146
PspGI CCWGG 6 cut(s) 169, 815, 1322, 1930, 2140, 2424
PspLI CGTACG 1 cut(s) 2639
PspN4I GGNNCC 3 cut(s) 31, 679, 1923
PspPI GGNCC 7 cut(s) 602, 668, 678, 1052, 1165, 1282, 1921
PstI CTGCAG 2 cut(s) 1264, 2776
PstNI CAGNNNCTG 1 cut(s) 2780
PsuI RGATCY 5 cut(s) 458, 648, 2015, 2153, 2294
PsyI GACNNNGTC 2 cut(s) 843, 2322
RseI CAYNNNNRTG 3 cut(s) 939, 1881, 2270
SalI GTCGAC 1 cut(s) 1818
Sau96I GGNCC 7 cut(s) 602, 668, 678, 1052, 1165, 1282, 1921
SchI GAGTC 6 cut(s) 740, 1921, 2168, 2392, 2744, 2762
ScrFI CCNGG 8 cut(s) 171, 817, 1324, 1932, 2142, 2332, 2426, 2967
SduI GDGCHC 1 cut(s) 2242
SexAI ACCWGGT 1 cut(s) 2424
SfaNI GCATC 3 cut(s) 1779, 1945, 2443
SfcI CTRYAG 4 cut(s) 1260, 2100, 2169, 2772
SfuI TTCGAA 1 cut(s) 556
SinI GGWCC 4 cut(s) 602, 668, 678, 1052
SmiMI CAYNNNNRTG 3 cut(s) 939, 1881, 2270
SmlI CTYRAG 8 cut(s) 314, 2120, 2375, 2400, 2457, 2680, 2809, 2902
SmoI CTYRAG 8 cut(s) 314, 2120, 2375, 2400, 2457, 2680, 2809, 2902
SpeI ACTAGT 1 cut(s) 1870
SphI GCATGC 2 cut(s) 1245, 2492
SsiI CCGC 8 cut(s) 419, 1245, 1485, 2806, 2867, 2899, 3161, 3186
SspI AATATT 1 cut(s) 2671
StyD4I CCNGG 8 cut(s) 169, 815, 1322, 1930, 2140, 2330, 2424, 2965
StyI CCWWGG 5 cut(s) 726, 1168, 1196, 1479, 2724
TaaI ACNGT 9 cut(s) 139, 251, 386, 1209, 1856, 1865, 1946, 2372, 2618
TaiI ACGT 8 cut(s) 346, 355, 517, 956, 974, 1097, 1139, 2927
TaqI TCGA 9 cut(s) 160, 437, 556, 734, 1177, 1552, 1572, 1819, 2312
TatI WGTACW 2 cut(s) 1204, 1543
TauI GCSGC 2 cut(s) 422, 3188
TscAI CASTG 5 cut(s) 19, 486, 1034, 1911, 2623
TseFI GTSAC 4 cut(s) 1334, 1352, 2814, 2907
TseI GCWGC 9 cut(s) 1262, 1913, 1979, 2275, 2540, 2708, 3091, 3182, 3188
Tsp45I GTSAC 4 cut(s) 1334, 1352, 2814, 2907
TspDTI ATGAA 9 cut(s) 17, 66, 111, 1293, 2150, 2220, 2532, 2675, 2681
TspGWI ACGGA 1 cut(s) 1371
TspRI CASTG 5 cut(s) 19, 486, 1034, 1911, 2623
Tth111I GACNNNGTC 2 cut(s) 843, 2322
Vha464I CTTAAG 2 cut(s) 314, 2375
VneI GTGCAC 1 cut(s) 2238
VpaK11BI GGWCC 4 cut(s) 602, 668, 678, 1052
VspI ATTAAT 1 cut(s) 900
XagI CCTNNNNNAGG 1 cut(s) 1059
XapI RAATTY 5 cut(s) 84, 484, 685, 911, 3026
XbaI TCTAGA 1 cut(s) 2741
XceI RCATGY 7 cut(s) 782, 924, 942, 1245, 1431, 1727, 2492
XcmI CCANNNNNNNNNTGG 1 cut(s) 1427
XmiI GTMKAC 3 cut(s) 349, 1819, 2336
XmnI GAANNNNTTC 1 cut(s) 1065
Zsp2I ATGCAT 1 cut(s) 1772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.