Rorug04G0001800

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002680
Physical Location & Seq
Forward (+)
384391 .. 391268
6878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0001800.1

Sequence Viewer

Length: 1254 bp
ATGTTGTCCATGGTCTTATGCGAGAACTTCATCTTTGTTAACAACCACAACAAGCAGACTTTGTTTTCGCACAAAATTGATTCGCAGTCGAGACTCGCAGCCGCAGCAGCAGTTGAGGTGGGTCGAGGACGGAGGTGCAGTGGGTTCGTTGCCAATGCCGGAAAGAGAAAACGGAAATGGTGGCAGAGCTTCTTCTTCGACGAGGAAGGTAATTGGCTTGGTTTGAAAGACGATGACATGCTGGCCGCGGAGGAAGAGGAGGAGGACTTGGATTCCAATTCCTACTCGGAAGCTGAGAAATTTGAGGCGTGGAAGACAAGGGCCGAAGCGATCGTGGATTTGAGGGAAGCACAGCAGGATATGAGCAACGAGGAGAATCGCAAGTGGGAGGATTGGCTTGCTGCGGATTCCAACTCGTCCTCGTCCTGGGAATACTTGCAGTCTGATATTCCTACCGAAACTGAAGCCGAGAAGGGGGTGCTTTACTCTCTCACCGATTCCCTTCTTGGAAGGGAAGATGATGATGATGACCTGCTCTACGAAGACCGTGTGTTCCGTTACGCCTCCCTCAATTCGGCTAAATTTTTGGCGGTTTTAATAATTATACCCTGCGCCTTGGATTTTGTGGTTCACGACTATGTTTTGATGCCCTTTCTGGACAGATATGTGAAGACCGTGCCACTTGCAGCAGAGATGCTGGATGTCAGAAGAAATCAAAAGCTTCAAATGGTTGATGAATTAAAAATTGAGAGAGCAAGATTTCAGCTTGAGATGGAAATTGGTAAAGCTCCACCACTTTCTGATGAGGAGGTCTGGTGGGAGTTGCGGCATAAAGCATTAGAGCTGAGAGATGAATGGAGATTTGAAAATCGTAGAGCATTTGCCAACATATGGTCCGATATGTCATTTGGGATCTCATTATTCATTCTTTTATACTGCAACCAGAGTAAAGTAGCTTTGCTGAAATTTACAGGTTATAAGATAATAAATAATATTTCAGATACTGGGAAGGCATTTCTAATTATACTCATCACAGATATTTTTTTAGGGTATCATTCTGAGTCTGGTTGGCAGACTTTGATAGAAATTTTTGTTGAGCATTATGGGCTTCAGGTTGATCAATCAGTTATAACCATTTTTATCTGCTTGATTCCGGTTATTATTGACGCATGCGTGAAACTTTGGCTGTTTAAGTTCCTTCCAAGATTATCCCCGAAGGTGGCAAACATATTCCGGGAGATGCAGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

417

Amino Acids

48.57

Weight (kDa)

4.87

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 186 - 415 2.5e-76 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 978, 1130
Acc36I ACCTGC 1 cut(s) 540
AccB7I CCANNNNNTGG 1 cut(s) 891
AccII CGCG 1 cut(s) 248
AciI CCGC 6 cut(s) 102, 246, 248, 404, 590, 826
AclWI GGATC 1 cut(s) 920
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 4 cut(s) 299, 581, 965, 1086
AcuI CTGAAG 2 cut(s) 483, 1094
AfiI CCNNNNNNNGG 5 cut(s) 426, 474, 574, 891, 1219
AgsI TTSAA 3 cut(s) 226, 725, 866
AjnI CCWGG 1 cut(s) 425
AluBI AGCT 7 cut(s) 189, 293, 721, 766, 788, 844, 956
AluI AGCT 7 cut(s) 189, 293, 721, 766, 788, 844, 956
Alw26I GTCTC 1 cut(s) 85
AlwI GGATC 1 cut(s) 920
AlwNI CAGNNNCTG 1 cut(s) 1004
AoxI GGCC 2 cut(s) 243, 321
ApeKI GCWGC 6 cut(s) 98, 104, 107, 401, 686, 1243
ApoI RAATTY 4 cut(s) 299, 581, 965, 1086
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
AspLEI GCGC 1 cut(s) 614
AspS9I GGNCC 2 cut(s) 321, 894
AsuC2I CCSGG 1 cut(s) 1235
AsuHPI GGTGA 1 cut(s) 484
AvaII GGWCC 1 cut(s) 894
BbsI GAAGAC 3 cut(s) 320, 549, 677
BbvI GCAGC 5 cut(s) 110, 116, 119, 388, 698
BccI CCATC 1 cut(s) 766
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 1 cut(s) 427
BclI TGATCA 1 cut(s) 1117
BcnI CCSGG 1 cut(s) 1235
BcoDI GTCTC 1 cut(s) 85
BfaI CTAG 1 cut(s) 1252
BfuAI ACCTGC 1 cut(s) 540
BisI GCNGC 9 cut(s) 99, 102, 105, 108, 246, 402, 687, 827, 1244
BlsI GCNGC 9 cut(s) 100, 103, 106, 109, 247, 403, 688, 828, 1245
Bme1390I CCNGG 2 cut(s) 427, 1235
Bme18I GGWCC 1 cut(s) 894
BmgT120I GGNCC 2 cut(s) 321, 894
BmrFI CCNGG 2 cut(s) 427, 1235
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 3 cut(s) 636, 684, 1230
BmuI ACTGGG 1 cut(s) 1014
BpiI GAAGAC 3 cut(s) 320, 549, 677
BpuEI CTTGAG 1 cut(s) 788
BpuMI CCSGG 1 cut(s) 1235
BsaJI CCNNGG 4 cut(s) 9, 246, 426, 615
BsaWI WCCGGW 1 cut(s) 1153
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 800, 830
Bsc4I CCNNNNNNNGG 5 cut(s) 426, 474, 574, 891, 1219
Bse1I ACTGG 1 cut(s) 1009
BseBI CCWGG 1 cut(s) 427
BseDI CCNNGG 4 cut(s) 9, 246, 426, 615
BseGI GGATG 1 cut(s) 706
BseLI CCNNNNNNNGG 5 cut(s) 426, 474, 574, 891, 1219
BseMII CTCAG 3 cut(s) 285, 836, 1050
BseNI ACTGG 1 cut(s) 1009
BseRI GAGGAG 4 cut(s) 272, 275, 386, 821
BseXI GCAGC 5 cut(s) 110, 116, 119, 388, 698
BsgI GTGCAG 1 cut(s) 157
Bsh1236I CGCG 1 cut(s) 248
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 2 cut(s) 245, 323
BsiEI CGRYCG 1 cut(s) 333
BsiSI CCGG 3 cut(s) 159, 1154, 1234
BslI CCNNNNNNNGG 5 cut(s) 426, 474, 574, 891, 1219
BsmAI GTCTC 1 cut(s) 85
BsnI GGCC 2 cut(s) 245, 323
Bsp143I GATC 3 cut(s) 330, 912, 1117
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 6 cut(s) 102, 246, 248, 404, 590, 826
BspANI GGCC 2 cut(s) 245, 323
BspCNI CTCAG 3 cut(s) 286, 837, 1051
BspFNI CGCG 1 cut(s) 248
BspMI ACCTGC 1 cut(s) 540
BspPI GGATC 1 cut(s) 920
BsrI ACTGG 1 cut(s) 1009
BssECI CCNNGG 4 cut(s) 9, 246, 426, 615
BssMI GATC 3 cut(s) 330, 912, 1117
BssT1I CCWWGG 2 cut(s) 9, 615
Bst2UI CCWGG 1 cut(s) 427
Bst4CI ACNGT 2 cut(s) 548, 676
Bst6I CTCTTC 1 cut(s) 249
BstC8I GCNNGC 3 cut(s) 243, 399, 1171
BstDEI CTNAG 3 cut(s) 294, 845, 1059
BstDSI CCRYGG 2 cut(s) 9, 246
BstF5I GGATG 1 cut(s) 706
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 614
BstKTI GATC 3 cut(s) 333, 915, 1120
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 3 cut(s) 330, 912, 1117
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 3 cut(s) 104, 107, 1105
BstNI CCWGG 1 cut(s) 427
BstNSI RCATGY 2 cut(s) 241, 1173
BstSCI CCNGG 2 cut(s) 425, 1233
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 5 cut(s) 110, 116, 119, 388, 698
BstV2I GAAGAC 3 cut(s) 320, 549, 677
BstX2I RGATCY 1 cut(s) 912
BstYI RGATCY 1 cut(s) 912
BsuRI GGCC 2 cut(s) 245, 323
BtgI CCRYGG 2 cut(s) 9, 246
BtsCI GGATG 1 cut(s) 706
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 145
BveI ACCTGC 1 cut(s) 540
Cac8I GCNNGC 3 cut(s) 243, 399, 1171
CaiI CAGNNNCTG 1 cut(s) 1004
CfoI GCGC 1 cut(s) 614
Cfr13I GGNCC 2 cut(s) 321, 894
Cfr42I CCGCGG 1 cut(s) 249
CseI GACGC 2 cut(s) 1175, 1235
CviAII CATG 3 cut(s) 10, 238, 1170
DdeI CTNAG 3 cut(s) 294, 845, 1059
DpnI GATC 3 cut(s) 332, 914, 1119
DpnII GATC 3 cut(s) 330, 912, 1117
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 2 cut(s) 9, 615
Eco47I GGWCC 1 cut(s) 894
Eco57I CTGAAG 2 cut(s) 483, 1094
EcoRII CCWGG 1 cut(s) 425
EcoT14I CCWWGG 2 cut(s) 9, 615
ErhI CCWWGG 2 cut(s) 9, 615
FaeI CATG 3 cut(s) 13, 241, 1173
FatI CATG 3 cut(s) 9, 237, 1169
FauNDI CATATG 1 cut(s) 890
FbaI TGATCA 1 cut(s) 1117
Fnu4HI GCNGC 9 cut(s) 99, 102, 105, 108, 246, 402, 687, 827, 1244
FokI GGATG 1 cut(s) 713
Fsp4HI GCNGC 9 cut(s) 99, 102, 105, 108, 246, 402, 687, 827, 1244
FspBI CTAG 1 cut(s) 1252
GlaI GCGC 1 cut(s) 613
GluI GCNGC 9 cut(s) 99, 102, 105, 108, 246, 402, 687, 827, 1244
HaeIII GGCC 2 cut(s) 245, 323
HapII CCGG 3 cut(s) 159, 1154, 1234
HgaI GACGC 2 cut(s) 1175, 1235
HhaI GCGC 1 cut(s) 614
Hin1II CATG 3 cut(s) 13, 241, 1173
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 719
HinfI GANTC 8 cut(s) 80, 93, 272, 376, 407, 497, 1061, 1150
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 3 cut(s) 159, 1154, 1234
HphI GGTGA 1 cut(s) 484
Hpy166II GTNNAC 2 cut(s) 40, 631
Hpy188I TCNGA 7 cut(s) 289, 445, 707, 802, 898, 1000, 1060
Hpy188III TCNNGA 3 cut(s) 90, 632, 656
Hpy8I GTNNAC 2 cut(s) 40, 631
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 7 cut(s) 200, 466, 504, 512, 1003, 1208, 1210
HpyCH4III ACNGT 2 cut(s) 548, 676
HpyCH4V TGCA 5 cut(s) 138, 439, 686, 939, 1243
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 107, 1105
HpyF3I CTNAG 3 cut(s) 294, 845, 1059
Hsp92II CATG 3 cut(s) 13, 241, 1173
HspAI GCGC 1 cut(s) 612
Ksp22I TGATCA 1 cut(s) 1117
KspAI GTTAAC 1 cut(s) 40
KspI CCGCGG 1 cut(s) 249
Kzo9I GATC 3 cut(s) 330, 912, 1117
LmnI GCTCC 1 cut(s) 793
Lsp1109I GCAGC 5 cut(s) 110, 116, 119, 388, 698
LweI GCATC 3 cut(s) 636, 684, 1230
MaeI CTAG 1 cut(s) 1252
MaeIII GTNAC 2 cut(s) 557, 1247
MalI GATC 3 cut(s) 332, 914, 1119
MboI GATC 3 cut(s) 330, 912, 1117
MboII GAAGA 8 cut(s) 184, 187, 266, 325, 527, 554, 682, 720
MflI RGATCY 1 cut(s) 912
MlyI GAGTC 2 cut(s) 87, 1070
MmeI TCCRAC 1 cut(s) 435
MseI TTAA 4 cut(s) 39, 596, 740, 1191
MslI CAYNNNNRTG 1 cut(s) 636
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 3 cut(s) 159, 1154, 1234
MspR9I CCNGG 2 cut(s) 427, 1235
MvaI CCWGG 1 cut(s) 427
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 3 cut(s) 104, 107, 1105
NciI CCSGG 1 cut(s) 1235
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 890
NdeII GATC 3 cut(s) 330, 912, 1117
NlaIII CATG 3 cut(s) 13, 241, 1173
NmeAIII GCCGAG 1 cut(s) 493
NmuCI GTSAC 1 cut(s) 1247
NspI RCATGY 2 cut(s) 241, 1173
PaeI GCATGC 1 cut(s) 1173
PfeI GAWTC 6 cut(s) 80, 272, 376, 407, 497, 1150
PflMI CCANNNNNTGG 1 cut(s) 891
PfoI TCCNGGA 1 cut(s) 1233
PkrI GCNGC 9 cut(s) 100, 103, 106, 109, 247, 403, 688, 828, 1245
Ple19I CGATCG 1 cut(s) 333
PleI GAGTC 2 cut(s) 87, 1069
PpsI GAGTC 2 cut(s) 87, 1069
PsiI TTATAA 2 cut(s) 978, 1130
Psp6I CCWGG 1 cut(s) 425
PspGI CCWGG 1 cut(s) 425
PspPI GGNCC 2 cut(s) 321, 894
PstNI CAGNNNCTG 1 cut(s) 1004
PsuI RGATCY 1 cut(s) 912
PvuI CGATCG 1 cut(s) 333
RseI CAYNNNNRTG 1 cut(s) 636
SacII CCGCGG 1 cut(s) 249
SaqAI TTAA 4 cut(s) 39, 596, 740, 1191
SatI GCNGC 9 cut(s) 99, 102, 105, 108, 246, 402, 687, 827, 1244
Sau3AI GATC 3 cut(s) 330, 912, 1117
Sau96I GGNCC 2 cut(s) 321, 894
SchI GAGTC 2 cut(s) 87, 1070
ScrFI CCNGG 2 cut(s) 427, 1235
SfaNI GCATC 3 cut(s) 636, 684, 1230
Sfr303I CCGCGG 1 cut(s) 249
SgrBI CCGCGG 1 cut(s) 249
SinI GGWCC 1 cut(s) 894
SmiMI CAYNNNNRTG 1 cut(s) 636
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
SphI GCATGC 1 cut(s) 1173
SsiI CCGC 6 cut(s) 102, 246, 248, 404, 590, 826
SspI AATATT 1 cut(s) 994
SspMI CTAG 1 cut(s) 1252
StyD4I CCNGG 2 cut(s) 425, 1233
StyI CCWWGG 2 cut(s) 9, 615
TaaI ACNGT 2 cut(s) 548, 676
TaqI TCGA 3 cut(s) 89, 124, 198
TauI GCSGC 3 cut(s) 104, 248, 829
TfiI GAWTC 6 cut(s) 80, 272, 376, 407, 497, 1150
Tru1I TTAA 4 cut(s) 39, 596, 740, 1191
Tru9I TTAA 4 cut(s) 39, 596, 740, 1191
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 1247
TseI GCWGC 6 cut(s) 98, 104, 107, 401, 686, 1243
Tsp45I GTSAC 1 cut(s) 1247
TspDTI ATGAA 4 cut(s) 19, 750, 867, 913
TspGWI ACGGA 3 cut(s) 145, 187, 545
TspRI CASTG 1 cut(s) 145
Van91I CCANNNNNTGG 1 cut(s) 891
VpaK11BI GGWCC 1 cut(s) 894
XapI RAATTY 4 cut(s) 299, 581, 965, 1086
XceI RCATGY 2 cut(s) 241, 1173
XspI CTAG 1 cut(s) 1252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.