Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002680

Physical Location & Seq

Genomic Coordinates Forward (+)

16106825 .. 16108608

Gene Structure 1784 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1075400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 921 bp

ATGTATGTTTATATCTTTATCTACTCCGGATTGATATATCTAGGCCACACTTGTGATGCCAAACCGCGAACAGTTATTGCATTGTGGAAAGTCAATCTGGAAGAACCAAAGTTACACCAGTCTGATCCATCAGTTGTGATTCATGATAACTCCACAAAAACACGCATCAAACTTGCAGCTGCAGTTGAGAATGACAAGCAACAACTTCAGTTGTTTTGCAATGCATGCAAAGGAATACCACAGGCTTGTCCTGCGTTTTTGGGTAACAACCAACCCCCGGTTGCTCCAGCAAGTGCACCACCAGCTGCAGAAGATTTAGAGTTGGCCATGGCTATTAGTGCCTCCATGCAGTCTGCTTTGCAGGAGAGACCATCCTTTCCTAATCCGCACCCTACCTATGAAGCAAGTTCATCCAGTAGTGATAATGGTTGGGCCGGAGCCTCCACAAGAACTGACAGTTACAATGGTGGTGGTGCACCAACTGCAGCTGCTGCTTCAAAAGTAAGTAGCGGCGAGTGGTCAGGGACTCAGAGTAATGAAGTTGGAGAGTCATCTCAGCAGGTGGAAATCCAGGATACCTCTTCCGTCCAAACAGCTCCTACTTCAGATATAATCCCATCAGCCCCACCGGTTGCAGATGATAGTCTAGATGCAGCTCCTGTTCACTATCCATCAATTGATTTCAGTCCTATTGATATCCCATCCCCAAGCCTTGAAAGCACACCTGCTAAAATTGATGAGAAGAAAGGTGAAAGCGGTTCATGCGTGATTTGTTTGGATGCTCCAGCTGAAGGAGCTTGCATCCCATGTGGACATATGGCTGGATGCATGTCCTGTTTGAAGGAGATTACAGGTAAGAAATGGGGTTGCCCCGTCTGTCGAGCCAAGATAGACCAAGTTATAAGGCTTTATGCTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_4 zf-C3HC4_3

Protein Analysis

307

Amino Acids

32.19

Weight (kDa)

4.89

Isoelectric Point (pI)

58.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	252 - 300	5.2e-14	Zinc finger, C3HC4 type (RING finger)
Prok-RING_4	PF14447	255 - 300	5.2e-06	Prokaryotic RING finger family 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002680)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23280 AT3G23280 AT4G14365
fragaria_vesca	FvH4_4g03720 FvH4_4g03720 FvH4_4g03730
malus_domestica	MD05G1075400.v1.1 MD13G1214800.v1.1 MD16G1219900.v1.1
prunus_persica	Prupe.1G040200_v2.0.a1 Prupe.1G040200_v2.0.a1
pyrus_communis	pycom13g18820 pycom16g18320
rosa_chinensis	RchiOBHm_Chr4g0393331 RchiOBHm_Chr4g0393371
rosa_laevigata	RLG00000009750 RLG00000009758 RLG00000009759 RLG00000009763
rosa_multiflora	Rmu_sc0008931.1_g000009
rosa_roxburghii	Rroxscaffold_3G00224380 Rroxscaffold_5G00338320 Rroxscaffold_5G00338340 Rroxscaffold_5G00338360 Rroxscaffold_5G00338390
rosa_rugosa	Rorug04G0001300 Rorug04G0001500 Rorug04G0001800
rosa_samantha	Rh4AG046800 Rh4AG047000 Rh4AG047300 Rh4AG047400 Rh4BG042400 Rh4BG042600 Rh4CG049900 Rh4CG050000 Rh4DG044200 Rh5AG327600
rosa_wichuraiana	Rw4G003700 Rw4G003720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	902
AarI	CACCTGC	2 cut(s)	550, 733
Acc36I	ACCTGC	2 cut(s)	550, 733
AccII	CGCG	1 cut(s)	67
AccIII	TCCGGA	1 cut(s)	26
AciI	CCGC	4 cut(s)	65, 386, 510, 756
AclWI	GGATC	1 cut(s)	119
AcoI	YGGCCR	1 cut(s)	324
AcuI	CTGAAG	3 cut(s)	191, 588, 810
AfiI	CCNNNNNNNGG	2 cut(s)	277, 791
AgeI	ACCGGT	1 cut(s)	628
AgsI	TTSAA	3 cut(s)	498, 716, 841
AjnI	CCWGG	1 cut(s)	570
AleI	CACNNNNGTG	1 cut(s)	51
AluBI	AGCT	7 cut(s)	179, 305, 488, 596, 656, 788, 797
AluI	AGCT	7 cut(s)	179, 305, 488, 596, 656, 788, 797
Alw21I	GWGCWC	2 cut(s)	298, 478
Alw26I	GTCTC	1 cut(s)	361
Alw44I	GTGCAC	2 cut(s)	294, 474
AlwI	GGATC	1 cut(s)	119
AlwNI	CAGNNNCTG	2 cut(s)	491, 659
Aor13HI	TCCGGA	1 cut(s)	26
AoxI	GGCC	3 cut(s)	43, 324, 432
ApaLI	GTGCAC	2 cut(s)	294, 474
ApeKI	GCWGC	7 cut(s)	176, 179, 305, 485, 488, 491, 653
AsiGI	ACCGGT	1 cut(s)	628
AspS9I	GGNCC	1 cut(s)	432
AsuC2I	CCSGG	1 cut(s)	278
AsuHPI	GGTGA	1 cut(s)	761
BaeGI	GKGCMC	2 cut(s)	298, 478
BalI	TGGCCA	1 cut(s)	326
Bbv12I	GWGCWC	2 cut(s)	298, 478
BbvI	GCAGC	7 cut(s)	166, 188, 292, 475, 478, 497, 665
BccI	CCATC	5 cut(s)	136, 379, 625, 679, 709
BciT130I	CCWGG	1 cut(s)	572
BciVI	GTATCC	1 cut(s)	568
BcnI	CCSGG	1 cut(s)	278
BcoDI	GTCTC	1 cut(s)	361
BfaI	CTAG	2 cut(s)	41, 647
BfmI	CTRYAG	3 cut(s)	180, 306, 483
BfuAI	ACCTGC	2 cut(s)	550, 733
BfuI	GTATCC	1 cut(s)	568
BisI	GCNGC	8 cut(s)	177, 180, 306, 486, 489, 492, 511, 654
BlsI	GCNGC	8 cut(s)	178, 181, 307, 487, 490, 493, 512, 655
Bme1390I	CCNGG	2 cut(s)	278, 572
BmgT120I	GGNCC	1 cut(s)	432
BmiI	GGNNCC	1 cut(s)	439
BmrFI	CCNGG	2 cut(s)	278, 572
BmsI	GCATC	6 cut(s)	46, 174, 640, 769, 810, 815
BpmI	CTGGAG	2 cut(s)	270, 768
BpuMI	CCSGG	1 cut(s)	278
BsaI	GGTCTC	1 cut(s)	361
BsaJI	CCNNGG	2 cut(s)	276, 327
BsaWI	WCCGGW	2 cut(s)	26, 628
Bsc4I	CCNNNNNNNGG	2 cut(s)	277, 791
Bse118I	RCCGGY	1 cut(s)	628
Bse1I	ACTGG	2 cut(s)	118, 414
Bse3DI	GCAATG	1 cut(s)	226
BseAI	TCCGGA	1 cut(s)	26
BseBI	CCWGG	1 cut(s)	572
BseDI	CCNNGG	2 cut(s)	276, 327
BseGI	GGATG	6 cut(s)	371, 410, 701, 784, 801, 830
BseLI	CCNNNNNNNGG	2 cut(s)	277, 791
BseMI	GCAATG	1 cut(s)	226
BseMII	CTCAG	2 cut(s)	542, 569
BseNI	ACTGG	2 cut(s)	118, 414
BseSI	GKGCMC	2 cut(s)	298, 478
BseXI	GCAGC	7 cut(s)	166, 188, 292, 475, 478, 497, 665
Bsh1236I	CGCG	1 cut(s)	67
BshFI	GGCC	3 cut(s)	45, 326, 434
BshTI	ACCGGT	1 cut(s)	628
BsiHKAI	GWGCWC	2 cut(s)	298, 478
BsiSI	CCGG	4 cut(s)	27, 278, 435, 629
BslFI	GGGAC	1 cut(s)	538
BslI	CCNNNNNNNGG	2 cut(s)	277, 791
BsmAI	GTCTC	1 cut(s)	361
BsmFI	GGGAC	1 cut(s)	538
BsnI	GGCC	3 cut(s)	45, 326, 434
Bso31I	GGTCTC	1 cut(s)	361
Bsp1286I	GDGCHC	2 cut(s)	298, 478
Bsp13I	TCCGGA	1 cut(s)	26
Bsp143I	GATC	1 cut(s)	124
Bsp19I	CCATGG	1 cut(s)	327
BspACI	CCGC	4 cut(s)	65, 386, 510, 756
BspANI	GGCC	3 cut(s)	45, 326, 434
BspCNI	CTCAG	2 cut(s)	541, 568
BspEI	TCCGGA	1 cut(s)	26
BspFNI	CGCG	1 cut(s)	67
BspHI	TCATGA	1 cut(s)	142
BspLI	GGNNCC	1 cut(s)	439
BspMAI	CTGCAG	3 cut(s)	184, 310, 487
BspMI	ACCTGC	2 cut(s)	550, 733
BspPI	GGATC	1 cut(s)	119
BspTNI	GGTCTC	1 cut(s)	361
BsrDI	GCAATG	1 cut(s)	226
BsrFI	RCCGGY	1 cut(s)	628
BsrI	ACTGG	2 cut(s)	118, 414
BssAI	RCCGGY	1 cut(s)	628
BssECI	CCNNGG	2 cut(s)	276, 327
BssMI	GATC	1 cut(s)	124
BssT1I	CCWWGG	1 cut(s)	327
Bst2UI	CCWGG	1 cut(s)	572
Bst4CI	ACNGT	2 cut(s)	73, 458
Bst6I	CTCTTC	1 cut(s)	586
BstAPI	GCANNNNNTGC	3 cut(s)	225, 482, 491
BstC8I	GCNNGC	2 cut(s)	226, 799
BstDEI	CTNAG	2 cut(s)	528, 555
BstDSI	CCRYGG	1 cut(s)	327
BstF5I	GGATG	6 cut(s)	371, 410, 701, 784, 801, 830
BstFNI	CGCG	1 cut(s)	67
BstKTI	GATC	1 cut(s)	127
BstMAI	GTCTC	1 cut(s)	361
BstMBI	GATC	1 cut(s)	124
BstMWI	GCNNNNNNNGC	9 cut(s)	225, 251, 302, 338, 482, 491, 717, 762, 794
BstNI	CCWGG	1 cut(s)	572
BstNSI	RCATGY	2 cut(s)	228, 832
BstSCI	CCNGG	2 cut(s)	276, 570
BstSFI	CTRYAG	3 cut(s)	180, 306, 483
BstSLI	GKGCMC	2 cut(s)	298, 478
BstUI	CGCG	1 cut(s)	67
BstV1I	GCAGC	7 cut(s)	166, 188, 292, 475, 478, 497, 665
BsuI	GTATCC	1 cut(s)	568
BsuRI	GGCC	3 cut(s)	45, 326, 434
BtgI	CCRYGG	1 cut(s)	327
BtsCI	GGATG	6 cut(s)	371, 410, 701, 784, 801, 830
BveI	ACCTGC	2 cut(s)	550, 733
Cac8I	GCNNGC	2 cut(s)	226, 799
CaiI	CAGNNNCTG	2 cut(s)	491, 659
CciI	TCATGA	1 cut(s)	142
Cfr10I	RCCGGY	1 cut(s)	628
Cfr13I	GGNCC	1 cut(s)	432
CspAI	ACCGGT	1 cut(s)	628
CspCI	CAANNNNNGTGG	2 cut(s)	451, 486
CviAII	CATG	7 cut(s)	143, 225, 328, 346, 762, 807, 829
DdeI	CTNAG	2 cut(s)	528, 555
DpnI	GATC	1 cut(s)	126
DpnII	GATC	1 cut(s)	124
EaeI	YGGCCR	1 cut(s)	324
Eam1104I	CTCTTC	1 cut(s)	586
EarI	CTCTTC	1 cut(s)	586
Eco130I	CCWWGG	1 cut(s)	327
Eco31I	GGTCTC	1 cut(s)	361
Eco32I	GATATC	1 cut(s)	697
Eco57I	CTGAAG	3 cut(s)	191, 588, 810
EcoRII	CCWGG	1 cut(s)	570
EcoRV	GATATC	1 cut(s)	697
EcoT14I	CCWWGG	1 cut(s)	327
EcoT22I	ATGCAT	2 cut(s)	226, 830
ErhI	CCWWGG	1 cut(s)	327
FaeI	CATG	7 cut(s)	146, 228, 331, 349, 765, 810, 832
FaqI	GGGAC	1 cut(s)	538
FatI	CATG	7 cut(s)	142, 224, 327, 345, 761, 806, 828
FauNDI	CATATG	1 cut(s)	816
Fnu4HI	GCNGC	8 cut(s)	177, 180, 306, 486, 489, 492, 511, 654
FokI	GGATG	6 cut(s)	358, 397, 688, 788, 791, 837
Fsp4HI	GCNGC	8 cut(s)	177, 180, 306, 486, 489, 492, 511, 654
FspBI	CTAG	2 cut(s)	41, 647
GluI	GCNGC	8 cut(s)	177, 180, 306, 486, 489, 492, 511, 654
GsuI	CTGGAG	2 cut(s)	270, 768
HaeIII	GGCC	3 cut(s)	45, 326, 434
HapII	CCGG	4 cut(s)	27, 278, 435, 629
Hin1II	CATG	7 cut(s)	146, 228, 331, 349, 765, 810, 832
HinfI	GANTC	3 cut(s)	139, 526, 548
HpaII	CCGG	4 cut(s)	27, 278, 435, 629
HphI	GGTGA	1 cut(s)	761
Hpy166II	GTNNAC	4 cut(s)	296, 476, 664, 812
Hpy188I	TCNGA	3 cut(s)	124, 531, 607
Hpy188III	TCNNGA	4 cut(s)	27, 98, 143, 647
Hpy8I	GTNNAC	4 cut(s)	296, 476, 664, 812
HpyAV	CCTTC	2 cut(s)	785, 835
HpyCH4III	ACNGT	2 cut(s)	73, 458
HpyF10VI	GCNNNNNNNGC	9 cut(s)	225, 251, 302, 338, 482, 491, 717, 762, 794
HpyF3I	CTNAG	2 cut(s)	528, 555
Hsp92II	CATG	7 cut(s)	146, 228, 331, 349, 765, 810, 832
Kpn2I	TCCGGA	1 cut(s)	26
Kzo9I	GATC	1 cut(s)	124
LmnI	GCTCC	6 cut(s)	289, 437, 601, 661, 787, 794
Lsp1109I	GCAGC	7 cut(s)	166, 188, 292, 475, 478, 497, 665
LweI	GCATC	6 cut(s)	46, 174, 640, 769, 810, 815
MaeI	CTAG	2 cut(s)	41, 647
MaeIII	GTNAC	3 cut(s)	111, 263, 458
MalI	GATC	1 cut(s)	126
MboI	GATC	1 cut(s)	124
MboII	GAAGA	4 cut(s)	113, 323, 573, 754
MfeI	CAATTG	1 cut(s)	675
MhlI	GDGCHC	2 cut(s)	298, 478
MlsI	TGGCCA	1 cut(s)	326
MluCI	AATT	2 cut(s)	675, 732
MluNI	TGGCCA	1 cut(s)	326
MlyI	GAGTC	2 cut(s)	520, 557
MmeI	TCCRAC	1 cut(s)	523
MnlI	CCTC	3 cut(s)	352, 451, 589
Mox20I	TGGCCA	1 cut(s)	326
Mph1103I	ATGCAT	2 cut(s)	226, 830
MroI	TCCGGA	1 cut(s)	26
MscI	TGGCCA	1 cut(s)	326
MslI	CAYNNNNRTG	1 cut(s)	51
Msp20I	TGGCCA	1 cut(s)	326
MspA1I	CMGCKG	4 cut(s)	179, 305, 488, 788
MspI	CCGG	4 cut(s)	27, 278, 435, 629
MspR9I	CCNGG	2 cut(s)	278, 572
MunI	CAATTG	1 cut(s)	675
MvaI	CCWGG	1 cut(s)	572
MvnI	CGCG	1 cut(s)	67
MwoI	GCNNNNNNNGC	9 cut(s)	225, 251, 302, 338, 482, 491, 717, 762, 794
NciI	CCSGG	1 cut(s)	278
NcoI	CCATGG	1 cut(s)	327
NdeI	CATATG	1 cut(s)	816
NdeII	GATC	1 cut(s)	124
NlaIII	CATG	7 cut(s)	146, 228, 331, 349, 765, 810, 832
NlaIV	GGNNCC	1 cut(s)	439
NsiI	ATGCAT	2 cut(s)	226, 830
NspI	RCATGY	2 cut(s)	228, 832
OliI	CACNNNNGTG	1 cut(s)	51
PaeI	GCATGC	1 cut(s)	228
PagI	TCATGA	1 cut(s)	142
PaqCI	CACCTGC	2 cut(s)	550, 733
PfeI	GAWTC	1 cut(s)	139
PfoI	TCCNGGA	1 cut(s)	570
PinAI	ACCGGT	1 cut(s)	628
PkrI	GCNGC	8 cut(s)	178, 181, 307, 487, 490, 493, 512, 655
PleI	GAGTC	2 cut(s)	520, 556
PpsI	GAGTC	2 cut(s)	520, 556
PsiI	TTATAA	1 cut(s)	902
Psp6I	CCWGG	1 cut(s)	570
PspGI	CCWGG	1 cut(s)	570
PspN4I	GGNNCC	1 cut(s)	439
PspPI	GGNCC	1 cut(s)	432
PsrI	GAACNNNNNNTAC	2 cut(s)	96, 128
PstI	CTGCAG	3 cut(s)	184, 310, 487
PstNI	CAGNNNCTG	2 cut(s)	491, 659
PvuII	CAGCTG	4 cut(s)	179, 305, 488, 788
RseI	CAYNNNNRTG	1 cut(s)	51
SatI	GCNGC	8 cut(s)	177, 180, 306, 486, 489, 492, 511, 654
Sau3AI	GATC	1 cut(s)	124
Sau96I	GGNCC	1 cut(s)	432
SchI	GAGTC	2 cut(s)	520, 557
ScrFI	CCNGG	2 cut(s)	278, 572
SduI	GDGCHC	2 cut(s)	298, 478
SfaNI	GCATC	6 cut(s)	46, 174, 640, 769, 810, 815
SfcI	CTRYAG	3 cut(s)	180, 306, 483
SmiMI	CAYNNNNRTG	1 cut(s)	51
SphI	GCATGC	1 cut(s)	228
Sse9I	AATT	2 cut(s)	675, 732
SsiI	CCGC	4 cut(s)	65, 386, 510, 756
SspMI	CTAG	2 cut(s)	41, 647
StyD4I	CCNGG	2 cut(s)	276, 570
StyI	CCWWGG	1 cut(s)	327
TaaI	ACNGT	2 cut(s)	73, 458
TaqI	TCGA	1 cut(s)	880
TasI	AATT	2 cut(s)	675, 732
TauI	GCSGC	1 cut(s)	513
TfiI	GAWTC	1 cut(s)	139
TseI	GCWGC	7 cut(s)	176, 179, 305, 485, 488, 491, 653
TspDTI	ATGAA	5 cut(s)	131, 399, 414, 552, 750
TspGWI	ACGGA	1 cut(s)	574
VneI	GTGCAC	2 cut(s)	294, 474
XbaI	TCTAGA	1 cut(s)	646
XceI	RCATGY	2 cut(s)	228, 832
XspI	CTAG	2 cut(s)	41, 647
Zsp2I	ATGCAT	2 cut(s)	226, 830

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.