Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002680

Physical Location & Seq

Genomic Coordinates Forward (+)

21839322 .. 21845119

Gene Structure 5798 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1219900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGGACTGCAGCAATCGAAAGATGAGTTGCTCCATCAGCAAGTCAGCAATGGAAACATCGAAGGGATTAAAGCACTCTGCAGAGACGGTGCAGGCCTGGAGTGGATTGATAGAGAGGGGAATACCCCTTTGATCGTGGCCTGTACAAATCATGGGCCCTATAACGTTGCAAAAACCTTGATTGAACTCGGTGCAAACGTCAACGCCTATCGCCCCGGTCGTCATGCTGGGACTCCTCTACATCATGCAGCTAAAAGAGGCCTAGAAGATGTTGTTAAATTACTTCTTTCGCATGGAGCAAATGCTTTAATCATGAATGATGACTGTCAAAGTCCTCTTGACGTTGCTAGGGCGAAGGGGCACACCAATGTTGTCCGCGCAATTGAGAGGCATATTTGCTTATTCTCTGGTTGGTTGCGGGAGTTTTATGGTCCTGGATTTCTTGAAGTACTTGCTCCTCAGTTGGTTTCAAGAAAAGTTTGGGTTGTTGTTTTGCCATGTAGTTCTCGCAAACCCACCAAGCCTTTCAAGTTGGAGCTTGCCATGTATCCTACCATGCAGGATGCCAAGCCACGGACAGTTATTGCATTGTGGAAAGTCAATCTGGAAGAACCAAAGTTACACCTGTCTGATCCTTCAGTTGTGATTCACGATAACGCCGCGATCCCAAGAGGCAGGAGGCGGAGAAGAAGCATGTACATCGCCCGAGAGGCTAGTAGTAGGCATCAAAAACTGCAAACACGTGTCAAACTTGCAGCTGCAGATGAAAATGACAAGCATCAACTTCAGTTGTTTTGCAATGCATGCAAAGGAATTCCACAGGCGTGTCCTGCATTTTTGGGTAACAACCAACCCCCAGTTGCTCCAGCAACGGCACCACCAGCGGCAGAAGATTTAGAGTTGGCCATGGCTATTAGTGCTTCCATTCAGTCTGCTATGCAGGAGAGACCATCCTTTCCTGATCCTCACCCAACCTATGAAGGAAGTGCATCCAGTAGTTATAATGGTTGGGCCGGAGCCTCTCCGAGTAATGACAGTTACAATGGTTGGGGTGTACCAACTGCCGCTGCTGCTCCAAAAGTAAGTAGCAGCAAGTGGTCAGGGACTGAGAGTAGCGAAGTTGGAGAGTCATCTCAGCAGGTGGAAATCCAGGATACCTCTTCTGTCCAAACAGCTCCTACCTCAGATATAATTCCATCAGCCCCACCAGTTGCAGATGATAGTCTAGATGCAGCTCCTATTCACTATCCATCAATTGATTTCAGTCCTATCGATATCCCATCCCCAAGCCTTGAAAGCACACCTGCTAAAGTTGAAGAGAAGAAGGGTGAAAGCGGTTCATGTGTGATTTGTTTGGATGCTCCAGTTGAAGGAGCTTGCATCCCTTGTGGACATATGGCTGGATGCATGTCCTGTTTGAAGGAGATTAAAGGCAAGAAATGGGGATGCCCCGTCTGTCGAGCCAAGATAGACCAGGTTATAAGGCTGTATGCTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_4 zf-C3HC4_3

Protein Analysis

500

Amino Acids

53.77

Weight (kDa)

6.93

Isoelectric Point (pI)

57.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	7 - 69	4.9e-08	Ankyrin repeats (3 copies)
Ank_5	PF13857	60 - 116	2.1e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	78 - 127	6.9e-07	Ankyrin repeats (3 copies)
Ank	PF00023	78 - 106	1.1e-06	Ankyrin repeat
zf-C3HC4_3	PF13920	445 - 493	1e-13	Zinc finger, C3HC4 type (RING finger)
Prok-RING_4	PF14447	448 - 493	1e-05	Prokaryotic RING finger family 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002680)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23280 AT3G23280 AT4G14365
fragaria_vesca	FvH4_4g03720 FvH4_4g03720 FvH4_4g03730
malus_domestica	MD05G1075400.v1.1 MD13G1214800.v1.1 MD16G1219900.v1.1
prunus_persica	Prupe.1G040200_v2.0.a1 Prupe.1G040200_v2.0.a1
pyrus_communis	pycom13g18820 pycom16g18320
rosa_chinensis	RchiOBHm_Chr4g0393331 RchiOBHm_Chr4g0393371
rosa_laevigata	RLG00000009750 RLG00000009758 RLG00000009759 RLG00000009763
rosa_multiflora	Rmu_sc0008931.1_g000009
rosa_roxburghii	Rroxscaffold_3G00224380 Rroxscaffold_5G00338320 Rroxscaffold_5G00338340 Rroxscaffold_5G00338360 Rroxscaffold_5G00338390
rosa_rugosa	Rorug04G0001300 Rorug04G0001500 Rorug04G0001800
rosa_samantha	Rh4AG046800 Rh4AG047000 Rh4AG047300 Rh4AG047400 Rh4BG042400 Rh4BG042600 Rh4CG049900 Rh4CG050000 Rh4DG044200 Rh5AG327600
rosa_wichuraiana	Rw4G003700 Rw4G003720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1002, 1481
AarI	CACCTGC	2 cut(s)	1129, 1312
Acc36I	ACCTGC	2 cut(s)	1129, 1312
AccB1I	GGYRCC	1 cut(s)	874
AccII	CGCG	2 cut(s)	378, 662
AciI	CCGC	7 cut(s)	376, 418, 660, 682, 884, 1065, 1335
AclI	AACGTT	1 cut(s)	165
AclWI	GGATC	3 cut(s)	626, 658, 956
AcoI	YGGCCR	1 cut(s)	903
AcsI	RAATTY	1 cut(s)	813
AcuI	CTGAAG	2 cut(s)	621, 770
AcvI	CACGTG	1 cut(s)	743
AfaI	GTAC	4 cut(s)	145, 450, 698, 1056
AfiI	CCNNNNNNNGG	2 cut(s)	573, 1370
AflIII	ACRYGT	2 cut(s)	740, 742
AgsI	TTSAA	8 cut(s)	185, 446, 471, 529, 1295, 1316, 1370, 1420
AjnI	CCWGG	4 cut(s)	96, 433, 1149, 1473
AleI	CACNNNNGTG	1 cut(s)	823
AluBI	AGCT	6 cut(s)	251, 538, 758, 1175, 1235, 1376
AluI	AGCT	6 cut(s)	251, 538, 758, 1175, 1235, 1376
Alw26I	GTCTC	2 cut(s)	78, 940
AlwI	GGATC	3 cut(s)	626, 658, 956
AlwNI	CAGNNNCTG	1 cut(s)	1106
Ama87I	CYCGRG	1 cut(s)	705
AoxI	GGCC	6 cut(s)	94, 138, 155, 259, 903, 1011
ApaI	GGGCCC	1 cut(s)	159
ApeKI	GCWGC	8 cut(s)	10, 248, 755, 758, 1067, 1070, 1089, 1232
ApoI	RAATTY	1 cut(s)	813
AspLEI	GCGC	1 cut(s)	380
AspS9I	GGNCC	4 cut(s)	155, 156, 431, 1011
AsuC2I	CCSGG	1 cut(s)	216
AsuHPI	GGTGA	2 cut(s)	959, 1340
AvaI	CYCGRG	1 cut(s)	705
AvaII	GGWCC	1 cut(s)	431
BaeGI	GKGCMC	2 cut(s)	159, 363
BalI	TGGCCA	1 cut(s)	905
BanI	GGYRCC	1 cut(s)	874
BanII	GRGCYC	1 cut(s)	159
BbrPI	CACGTG	1 cut(s)	743
BbvI	GCAGC	8 cut(s)	22, 260, 745, 767, 1054, 1057, 1101, 1244
BccI	CCATC	5 cut(s)	42, 958, 1204, 1258, 1288
BceAI	ACGGC	1 cut(s)	888
BcgI	CGANNNNNNTGC	2 cut(s)	682, 716
BciT130I	CCWGG	4 cut(s)	98, 435, 1151, 1475
BciVI	GTATCC	2 cut(s)	558, 1147
BcnI	CCSGG	1 cut(s)	216
BcoDI	GTCTC	2 cut(s)	78, 940
BfaI	CTAG	4 cut(s)	263, 348, 714, 1226
BfmI	CTRYAG	3 cut(s)	8, 79, 759
BfuAI	ACCTGC	2 cut(s)	1129, 1312
BfuI	GTATCC	2 cut(s)	558, 1147
BglI	GCCNNNNNGGC	1 cut(s)	710
BmcAI	AGTACT	1 cut(s)	450
Bme1390I	CCNGG	5 cut(s)	98, 216, 435, 1151, 1475
Bme18I	GGWCC	1 cut(s)	431
BmeT110I	CYCGRG	1 cut(s)	705
BmgT120I	GGNCC	4 cut(s)	155, 156, 431, 1011
BmiI	GGNNCC	3 cut(s)	157, 876, 1018
BmrFI	CCNGG	5 cut(s)	98, 216, 435, 1151, 1475
BmrI	ACTGGG	1 cut(s)	851
BmsI	GCATC	9 cut(s)	553, 733, 787, 998, 1219, 1348, 1389, 1394, 1436
BmuI	ACTGGG	1 cut(s)	851
BpmI	CTGGAG	3 cut(s)	119, 849, 1347
BpuMI	CCSGG	1 cut(s)	216
Bsa29I	ATCGAT	1 cut(s)	1272
BsaAI	YACGTR	1 cut(s)	743
BsaI	GGTCTC	1 cut(s)	940
BsaJI	CCNNGG	3 cut(s)	214, 572, 906
Bsc4I	CCNNNNNNNGG	2 cut(s)	573, 1370
Bse1I	ACTGG	4 cut(s)	857, 993, 1208, 1364
Bse3DI	GCAATG	2 cut(s)	55, 805
BseBI	CCWGG	4 cut(s)	98, 435, 1151, 1475
BseCI	ATCGAT	1 cut(s)	1272
BseDI	CCNNGG	3 cut(s)	214, 572, 906
BseGI	GGATG	8 cut(s)	568, 950, 989, 1280, 1363, 1380, 1409, 1451
BseLI	CCNNNNNNNGG	2 cut(s)	573, 1370
BseMI	GCAATG	2 cut(s)	55, 805
BseMII	CTCAG	4 cut(s)	473, 1098, 1148, 1197
BseNI	ACTGG	4 cut(s)	857, 993, 1208, 1364
BseRI	GAGGAG	2 cut(s)	225, 447
BseSI	GKGCMC	2 cut(s)	159, 363
BseXI	GCAGC	8 cut(s)	22, 260, 745, 767, 1054, 1057, 1101, 1244
BseYI	CCCAGC	1 cut(s)	227
BsgI	GTGCAG	1 cut(s)	111
Bsh1236I	CGCG	2 cut(s)	378, 662
Bsh1285I	CGRYCG	1 cut(s)	220
BshFI	GGCC	6 cut(s)	96, 140, 157, 261, 905, 1013
BshNI	GGYRCC	1 cut(s)	874
BshVI	ATCGAT	1 cut(s)	1272
BsiEI	CGRYCG	1 cut(s)	220
BsiHKCI	CYCGRG	1 cut(s)	705
BsiSI	CCGG	2 cut(s)	216, 1014
BslFI	GGGAC	3 cut(s)	18, 244, 1117
BslI	CCNNNNNNNGG	2 cut(s)	573, 1370
BsmAI	GTCTC	2 cut(s)	78, 940
BsmBI	CGTCTC	1 cut(s)	78
BsmFI	GGGAC	3 cut(s)	18, 244, 1117
BsnI	GGCC	6 cut(s)	96, 140, 157, 261, 905, 1013
Bso31I	GGTCTC	1 cut(s)	940
BsoBI	CYCGRG	1 cut(s)	705
Bsp120I	GGGCCC	1 cut(s)	155
Bsp1286I	GDGCHC	2 cut(s)	159, 363
Bsp1407I	TGTACA	2 cut(s)	143, 696
Bsp143I	GATC	4 cut(s)	132, 631, 663, 961
Bsp19I	CCATGG	1 cut(s)	906
BspACI	CCGC	7 cut(s)	376, 418, 660, 682, 884, 1065, 1335
BspANI	GGCC	6 cut(s)	96, 140, 157, 261, 905, 1013
BspCNI	CTCAG	4 cut(s)	472, 1099, 1147, 1196
BspDI	ATCGAT	1 cut(s)	1272
BspFNI	CGCG	2 cut(s)	378, 662
BspHI	TCATGA	1 cut(s)	312
BspLI	GGNNCC	3 cut(s)	157, 876, 1018
BspMAI	CTGCAG	3 cut(s)	12, 83, 763
BspMI	ACCTGC	2 cut(s)	1129, 1312
BspPI	GGATC	3 cut(s)	626, 658, 956
BspT107I	GGYRCC	1 cut(s)	874
BspTNI	GGTCTC	1 cut(s)	940
BsrDI	GCAATG	2 cut(s)	55, 805
BsrGI	TGTACA	2 cut(s)	143, 696
BsrI	ACTGG	4 cut(s)	857, 993, 1208, 1364
BssECI	CCNNGG	3 cut(s)	214, 572, 906
BssMI	GATC	4 cut(s)	132, 631, 663, 961
BssT1I	CCWWGG	1 cut(s)	906
Bst2UI	CCWGG	4 cut(s)	98, 435, 1151, 1475
Bst4CI	ACNGT	4 cut(s)	89, 326, 580, 1037
Bst6I	CTCTTC	2 cut(s)	1165, 1311
BstAPI	GCANNNNNTGC	1 cut(s)	804
BstAUI	TGTACA	2 cut(s)	143, 696
BstBAI	YACGTR	1 cut(s)	743
BstC8I	GCNNGC	4 cut(s)	94, 540, 805, 1378
BstDEI	CTNAG	4 cut(s)	459, 1107, 1134, 1183
BstDSI	CCRYGG	2 cut(s)	572, 906
BstF5I	GGATG	8 cut(s)	568, 950, 989, 1280, 1363, 1380, 1409, 1451
BstFNI	CGCG	2 cut(s)	378, 662
BstHHI	GCGC	1 cut(s)	380
BstKTI	GATC	4 cut(s)	135, 634, 666, 964
BstMAI	GTCTC	2 cut(s)	78, 940
BstMBI	GATC	4 cut(s)	132, 631, 663, 961
BstMCI	CGRYCG	1 cut(s)	220
BstMWI	GCNNNNNNNGC	8 cut(s)	37, 710, 804, 830, 881, 917, 1070, 1296
BstNI	CCWGG	4 cut(s)	98, 435, 1151, 1475
BstNSI	RCATGY	3 cut(s)	697, 807, 1411
BstSCI	CCNGG	5 cut(s)	96, 214, 433, 1149, 1473
BstSFI	CTRYAG	3 cut(s)	8, 79, 759
BstSLI	GKGCMC	2 cut(s)	159, 363
BstUI	CGCG	2 cut(s)	378, 662
BstV1I	GCAGC	8 cut(s)	22, 260, 745, 767, 1054, 1057, 1101, 1244
Bsu15I	ATCGAT	1 cut(s)	1272
BsuI	GTATCC	2 cut(s)	558, 1147
BsuRI	GGCC	6 cut(s)	96, 140, 157, 261, 905, 1013
BsuTUI	ATCGAT	1 cut(s)	1272
BtgI	CCRYGG	2 cut(s)	572, 906
BtgZI	GCGATG	1 cut(s)	685
BtsCI	GGATG	8 cut(s)	568, 950, 989, 1280, 1363, 1380, 1409, 1451
BveI	ACCTGC	2 cut(s)	1129, 1312
Cac8I	GCNNGC	4 cut(s)	94, 540, 805, 1378
CaiI	CAGNNNCTG	1 cut(s)	1106
CciI	TCATGA	1 cut(s)	312
CfoI	GCGC	1 cut(s)	380
Cfr13I	GGNCC	4 cut(s)	155, 156, 431, 1011
ClaI	ATCGAT	1 cut(s)	1272
CsiI	ACCWGGT	1 cut(s)	1473
Csp6I	GTAC	4 cut(s)	144, 449, 697, 1055
CviQI	GTAC	4 cut(s)	144, 449, 697, 1055
DdeI	CTNAG	4 cut(s)	459, 1107, 1134, 1183
DpnI	GATC	4 cut(s)	134, 633, 665, 963
DpnII	GATC	4 cut(s)	132, 631, 663, 961
EaeI	YGGCCR	1 cut(s)	903
Eam1104I	CTCTTC	2 cut(s)	1165, 1311
EarI	CTCTTC	2 cut(s)	1165, 1311
EciI	GGCGGA	1 cut(s)	697
Eco130I	CCWWGG	1 cut(s)	906
Eco147I	AGGCCT	2 cut(s)	96, 261
Eco24I	GRGCYC	1 cut(s)	159
Eco31I	GGTCTC	1 cut(s)	940
Eco32I	GATATC	1 cut(s)	1276
Eco47I	GGWCC	1 cut(s)	431
Eco57I	CTGAAG	2 cut(s)	621, 770
Eco72I	CACGTG	1 cut(s)	743
Eco88I	CYCGRG	1 cut(s)	705
EcoO109I	RGGNCCY	1 cut(s)	156
EcoRI	GAATTC	1 cut(s)	813
EcoRII	CCWGG	4 cut(s)	96, 433, 1149, 1473
EcoRV	GATATC	1 cut(s)	1276
EcoT14I	CCWWGG	1 cut(s)	906
EcoT22I	ATGCAT	2 cut(s)	805, 1409
EcoT38I	GRGCYC	1 cut(s)	159
ErhI	CCWWGG	1 cut(s)	906
Esp3I	CGTCTC	1 cut(s)	78
FaqI	GGGAC	3 cut(s)	18, 244, 1117
FauI	CCCGC	1 cut(s)	411
FauNDI	CATATG	1 cut(s)	1395
FokI	GGATG	8 cut(s)	575, 937, 976, 1267, 1367, 1370, 1416, 1458
FriOI	GRGCYC	1 cut(s)	159
FspBI	CTAG	4 cut(s)	263, 348, 714, 1226
GlaI	GCGC	1 cut(s)	379
GsaI	CCCAGC	1 cut(s)	231
GsuI	CTGGAG	3 cut(s)	119, 849, 1347
HaeIII	GGCC	6 cut(s)	96, 140, 157, 261, 905, 1013
HapII	CCGG	2 cut(s)	216, 1014
HhaI	GCGC	1 cut(s)	380
Hin6I	GCGC	1 cut(s)	378
HinP1I	GCGC	1 cut(s)	378
HincII	GTYRAC	1 cut(s)	202
HindII	GTYRAC	1 cut(s)	202
HinfI	GANTC	3 cut(s)	232, 646, 1127
HpaII	CCGG	2 cut(s)	216, 1014
HphI	GGTGA	2 cut(s)	959, 1340
Hpy166II	GTNNAC	3 cut(s)	202, 1055, 1391
Hpy188I	TCNGA	3 cut(s)	631, 1026, 1186
Hpy188III	TCNNGA	8 cut(s)	313, 338, 443, 471, 605, 650, 959, 1226
Hpy8I	GTNNAC	3 cut(s)	202, 1055, 1391
HpyAV	CCTTC	7 cut(s)	56, 349, 645, 974, 1318, 1364, 1414
HpyCH4III	ACNGT	4 cut(s)	89, 326, 580, 1037
HpyCH4IV	ACGT	4 cut(s)	165, 198, 342, 742
HpyF10VI	GCNNNNNNNGC	8 cut(s)	37, 710, 804, 830, 881, 917, 1070, 1296
HpyF3I	CTNAG	4 cut(s)	459, 1107, 1134, 1183
HpySE526I	ACGT	4 cut(s)	165, 198, 342, 742
HspAI	GCGC	1 cut(s)	378
Kzo9I	GATC	4 cut(s)	132, 631, 663, 961
Lsp1109I	GCAGC	8 cut(s)	22, 260, 745, 767, 1054, 1057, 1101, 1244
LweI	GCATC	9 cut(s)	553, 733, 787, 998, 1219, 1348, 1389, 1394, 1436
MabI	ACCWGGT	1 cut(s)	1473
MaeI	CTAG	4 cut(s)	263, 348, 714, 1226
MaeII	ACGT	4 cut(s)	165, 198, 342, 742
MaeIII	GTNAC	3 cut(s)	618, 842, 1037
MalI	GATC	4 cut(s)	134, 633, 665, 963
MboI	GATC	4 cut(s)	132, 631, 663, 961
MboII	GAAGA	7 cut(s)	278, 620, 699, 902, 1152, 1328, 1333
MfeI	CAATTG	2 cut(s)	381, 1254
MhlI	GDGCHC	2 cut(s)	159, 363
MlsI	TGGCCA	1 cut(s)	905
MluCI	AATT	5 cut(s)	278, 381, 813, 1191, 1254
MluNI	TGGCCA	1 cut(s)	905
MlyI	GAGTC	2 cut(s)	226, 1136
MmeI	TCCRAC	2 cut(s)	513, 1102
Mox20I	TGGCCA	1 cut(s)	905
Mph1103I	ATGCAT	2 cut(s)	805, 1409
MscI	TGGCCA	1 cut(s)	905
MseI	TTAA	4 cut(s)	69, 276, 308, 1428
MslI	CAYNNNNRTG	2 cut(s)	366, 823
Msp20I	TGGCCA	1 cut(s)	905
MspA1I	CMGCKG	3 cut(s)	758, 884, 1067
MspI	CCGG	2 cut(s)	216, 1014
MspR9I	CCNGG	5 cut(s)	98, 216, 435, 1151, 1475
MunI	CAATTG	2 cut(s)	381, 1254
MvaI	CCWGG	4 cut(s)	98, 435, 1151, 1475
MvnI	CGCG	2 cut(s)	378, 662
MwoI	GCNNNNNNNGC	8 cut(s)	37, 710, 804, 830, 881, 917, 1070, 1296
NciI	CCSGG	1 cut(s)	216
NcoI	CCATGG	1 cut(s)	906
NdeI	CATATG	1 cut(s)	1395
NdeII	GATC	4 cut(s)	132, 631, 663, 961
NlaIV	GGNNCC	3 cut(s)	157, 876, 1018
NsiI	ATGCAT	2 cut(s)	805, 1409
NspI	RCATGY	3 cut(s)	697, 807, 1411
OliI	CACNNNNGTG	1 cut(s)	823
PaeI	GCATGC	1 cut(s)	807
PagI	TCATGA	1 cut(s)	312
PaqCI	CACCTGC	2 cut(s)	1129, 1312
PceI	AGGCCT	2 cut(s)	96, 261
PcsI	WCGNNNNNNNCGW	2 cut(s)	195, 217
PfeI	GAWTC	1 cut(s)	646
PfoI	TCCNGGA	2 cut(s)	433, 1149
PleI	GAGTC	2 cut(s)	226, 1135
PmaCI	CACGTG	1 cut(s)	743
PmlI	CACGTG	1 cut(s)	743
PpsI	GAGTC	2 cut(s)	226, 1135
Ppu21I	YACGTR	1 cut(s)	743
PsiI	TTATAA	2 cut(s)	1002, 1481
Psp1406I	AACGTT	1 cut(s)	165
Psp6I	CCWGG	4 cut(s)	96, 433, 1149, 1473
PspCI	CACGTG	1 cut(s)	743
PspFI	CCCAGC	1 cut(s)	227
PspGI	CCWGG	4 cut(s)	96, 433, 1149, 1473
PspN4I	GGNNCC	3 cut(s)	157, 876, 1018
PspOMI	GGGCCC	1 cut(s)	155
PspPI	GGNCC	4 cut(s)	155, 156, 431, 1011
PsrI	GAACNNNNNNTAC	2 cut(s)	603, 635
PstI	CTGCAG	3 cut(s)	12, 83, 763
PstNI	CAGNNNCTG	1 cut(s)	1106
PvuII	CAGCTG	1 cut(s)	758
RsaI	GTAC	4 cut(s)	145, 450, 698, 1056
RsaNI	GTAC	4 cut(s)	144, 449, 697, 1055
RseI	CAYNNNNRTG	2 cut(s)	366, 823
SaqAI	TTAA	4 cut(s)	69, 276, 308, 1428
Sau3AI	GATC	4 cut(s)	132, 631, 663, 961
Sau96I	GGNCC	4 cut(s)	155, 156, 431, 1011
ScaI	AGTACT	1 cut(s)	450
SchI	GAGTC	2 cut(s)	226, 1136
ScrFI	CCNGG	5 cut(s)	98, 216, 435, 1151, 1475
SduI	GDGCHC	2 cut(s)	159, 363
SexAI	ACCWGGT	1 cut(s)	1473
SfaNI	GCATC	9 cut(s)	553, 733, 787, 998, 1219, 1348, 1389, 1394, 1436
SfcI	CTRYAG	3 cut(s)	8, 79, 759
SinI	GGWCC	1 cut(s)	431
SmiMI	CAYNNNNRTG	2 cut(s)	366, 823
SphI	GCATGC	1 cut(s)	807
Sse9I	AATT	5 cut(s)	278, 381, 813, 1191, 1254
SseBI	AGGCCT	2 cut(s)	96, 261
SsiI	CCGC	7 cut(s)	376, 418, 660, 682, 884, 1065, 1335
SspMI	CTAG	4 cut(s)	263, 348, 714, 1226
StuI	AGGCCT	2 cut(s)	96, 261
StyD4I	CCNGG	5 cut(s)	96, 214, 433, 1149, 1473
StyI	CCWWGG	1 cut(s)	906
TaaI	ACNGT	4 cut(s)	89, 326, 580, 1037
TaiI	ACGT	4 cut(s)	168, 201, 345, 745
TaqI	TCGA	4 cut(s)	17, 60, 1272, 1459
TasI	AATT	5 cut(s)	278, 381, 813, 1191, 1254
TatI	WGTACW	3 cut(s)	143, 448, 696
TauI	GCSGC	3 cut(s)	662, 887, 1067
TfiI	GAWTC	1 cut(s)	646
Tru1I	TTAA	4 cut(s)	69, 276, 308, 1428
Tru9I	TTAA	4 cut(s)	69, 276, 308, 1428
TseI	GCWGC	8 cut(s)	10, 248, 755, 758, 1067, 1070, 1089, 1232
TspDTI	ATGAA	4 cut(s)	329, 780, 993, 1329
TspGWI	ACGGA	1 cut(s)	589
VpaK11BI	GGWCC	1 cut(s)	431
XapI	RAATTY	1 cut(s)	813
XbaI	TCTAGA	1 cut(s)	1225
XceI	RCATGY	3 cut(s)	697, 807, 1411
XspI	CTAG	4 cut(s)	263, 348, 714, 1226
ZrmI	AGTACT	1 cut(s)	450
Zsp2I	ATGCAT	2 cut(s)	805, 1409

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.