Rroxscaffold_5G00338360

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002680
Physical Location & Seq
Forward (+)
6513601 .. 6517553
3953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00338360.1

Sequence Viewer

Length: 1053 bp
ATGGGGTCGTCTTCCTCCCAGAGAATCAACATTATTCAGAAACTCGAAGTCGATAACCGTTTTCCTCTGCGTTTTTACTTTCGGATGGCTGATATATTTCTCAGACAGGCTGACATATATCGGGAACAGAAAGATATTTTTTACCTGTACATTACGCTCCTAAGGTTTGCAAGTTTGGTCTCCGAGACAATACCGGATCATCAAGACTATATAAAGGCTGATCAAAGAGAAAGATCCTCTCAGAAGGAAGTGAAAAAGAAATTGTTGAATGCTCTTGCTGAGCTAGAGGAGTTAAAACCAGCAGTTAAACAGAAGATCAATGAGATAAACAGAGGAAATGCATATCAAAACAGCAGGCATGATCACCAAACTGGGGAAAGTATACCTCGTAGATCTGATAAAAAGAAAGTCGAAAGTGTGGGGACTTGTAGCTCCAGTACTGGTGGAGAGAATTTATCCCCACACCCAACACCAGAGTTTCCCTTTGGTCTCCATGGTAACAAGAGATTAAAGAAAGCACAGTATGTATCTTTCTCAGAGACACGAACAAAAATTTTACTTGAAGAGGCACATGATTGGAAGCTTACCACTCCATGCAAGCCAATACCACAGGCGCGTCCTGCAATTAACCAGCCAACTGCACCACGAGTTGAAGAAGATTTAGAACTGGTCCAGGCTATTGATGCCTCCATCCAGTCTGCTTTGCAGGAGAGGCCGCCCTTGTCTGATGCACTCCGAATCCATGAAACAAATGCTTCAAGTAGTTCGAATTCAATCCCATCATCTCCAACCCCATCAGCTCCACCAGTTTCAGATGATTGTCTAAATGATCCTTTTCGGTGTCGATCAACTGATTCCAGTCCTATCCAGGAAGGTGAAAATGCTTCTTCTTCATGTGTGATATGTATGGATTCTCCAGTTGAAGGAGCTTTTATCCCTTGTGGGCATATGGCTGGATGCATGGCTTGTTTGAATGACATTAAAGATAAGAACTGGGATTGCCCTGTCTGCCGCGCTAAAGTCGATCAGGTCATAAAGCTCTATACTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Methyltransf_29 zf-C3HC4_3

Protein Analysis

350

Amino Acids

39.25

Weight (kDa)

6.72

Isoelectric Point (pI)

55.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
USP8_dimer PF08969 13 - 102 2.7e-10 USP8 dimerisation domain
zf-C3HC4_3 PF13920 297 - 344 1.3e-14 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 298 - 338 9.3e-06 Ring finger domain
Prok-RING_4 PF14447 299 - 343 5.8e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 382
AccII CGCG 2 cut(s) 616, 1014
AciI CCGC 2 cut(s) 716, 1012
AclWI GGATC 3 cut(s) 204, 228, 824
AcsI RAATTY 3 cut(s) 451, 552, 769
AfaI GTAC 2 cut(s) 149, 439
AfiI CCNNNNNNNGG 2 cut(s) 373, 923
AgsI TTSAA 7 cut(s) 268, 563, 653, 759, 774, 923, 973
AjnI CCWGG 2 cut(s) 672, 867
AluBI AGCT 6 cut(s) 283, 432, 583, 800, 929, 1039
AluI AGCT 6 cut(s) 283, 432, 583, 800, 929, 1039
Alw26I GTCTC 4 cut(s) 179, 184, 494, 533
AlwI GGATC 3 cut(s) 204, 228, 824
AoxI GGCC 1 cut(s) 713
ApoI RAATTY 3 cut(s) 451, 552, 769
AspLEI GCGC 2 cut(s) 616, 1016
AspS9I GGNCC 1 cut(s) 670
AsuHPI GGTGA 2 cut(s) 356, 887
AsuII TTCGAA 1 cut(s) 767
AvaII GGWCC 1 cut(s) 670
AxyI CCTNAGG 1 cut(s) 161
BauI CACGAG 1 cut(s) 645
BbsI GAAGAC 1 cut(s) 3
BccI CCATC 4 cut(s) 79, 698, 787, 802
BciT130I CCWGG 2 cut(s) 674, 869
BclI TGATCA 2 cut(s) 220, 361
BcoDI GTCTC 4 cut(s) 179, 184, 494, 533
BfaI CTAG 1 cut(s) 284
BglII AGATCT 1 cut(s) 392
BisI GCNGC 2 cut(s) 716, 1012
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 2 cut(s) 717, 1013
BmcAI AGTACT 1 cut(s) 439
Bme1390I CCNGG 2 cut(s) 674, 869
Bme18I GGWCC 1 cut(s) 670
BmgT120I GGNCC 1 cut(s) 670
BmrFI CCNGG 2 cut(s) 674, 869
BmrI ACTGGG 2 cut(s) 381, 1003
BmsI GCATC 3 cut(s) 673, 718, 947
BmuI ACTGGG 2 cut(s) 381, 1003
BpiI GAAGAC 1 cut(s) 3
BpmI CTGGAG 2 cut(s) 418, 900
Bpu1102I GCTNAGC 1 cut(s) 279
Bpu14I TTCGAA 1 cut(s) 767
BsaI GGTCTC 2 cut(s) 184, 494
BsaJI CCNNGG 1 cut(s) 493
BsaWI WCCGGW 1 cut(s) 193
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 923
Bse1I ACTGG 9 cut(s) 376, 435, 445, 672, 694, 806, 858, 917, 998
Bse21I CCTNAGG 1 cut(s) 161
BseBI CCWGG 2 cut(s) 674, 869
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 3 cut(s) 90, 690, 962
BseLI CCNNNNNNNGG 2 cut(s) 373, 923
BseMII CTCAG 4 cut(s) 115, 254, 270, 549
BseNI ACTGG 9 cut(s) 376, 435, 445, 672, 694, 806, 858, 917, 998
BseRI GAGGAG 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 624
Bsh1236I CGCG 2 cut(s) 616, 1014
BshFI GGCC 1 cut(s) 715
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 2 cut(s) 373, 923
BsmAI GTCTC 4 cut(s) 179, 184, 494, 533
BsmFI GGGAC 1 cut(s) 436
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 1 cut(s) 715
Bso31I GGTCTC 2 cut(s) 184, 494
Bsp119I TTCGAA 1 cut(s) 767
Bsp1407I TGTACA 1 cut(s) 147
Bsp143I GATC 9 cut(s) 196, 220, 233, 315, 361, 392, 829, 845, 1024
Bsp1720I GCTNAGC 1 cut(s) 279
Bsp19I CCATGG 1 cut(s) 493
BspACI CCGC 2 cut(s) 716, 1012
BspANI GGCC 1 cut(s) 715
BspCNI CTCAG 4 cut(s) 114, 253, 271, 548
BspFNI CGCG 2 cut(s) 616, 1014
BspPI GGATC 3 cut(s) 204, 228, 824
BspT104I TTCGAA 1 cut(s) 767
BspTNI GGTCTC 2 cut(s) 184, 494
BsrGI TGTACA 1 cut(s) 147
BsrI ACTGG 9 cut(s) 376, 435, 445, 672, 694, 806, 858, 917, 998
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 9 cut(s) 196, 220, 233, 315, 361, 392, 829, 845, 1024
BssNAI GTATAC 1 cut(s) 383
BssSI CACGAG 1 cut(s) 645
BssT1I CCWWGG 1 cut(s) 493
Bst1107I GTATAC 1 cut(s) 383
Bst2BI CACGAG 1 cut(s) 645
Bst2UI CCWGG 2 cut(s) 674, 869
Bst4CI ACNGT 3 cut(s) 59, 522, 1048
Bst6I CTCTTC 1 cut(s) 558
BstAUI TGTACA 1 cut(s) 147
BstBI TTCGAA 1 cut(s) 767
BstC8I GCNNGC 2 cut(s) 356, 599
BstDEI CTNAG 5 cut(s) 101, 161, 240, 279, 535
BstDSI CCRYGG 1 cut(s) 493
BstF5I GGATG 3 cut(s) 90, 690, 962
BstFNI CGCG 2 cut(s) 616, 1014
BstHHI GCGC 2 cut(s) 616, 1016
BstKTI GATC 9 cut(s) 199, 223, 236, 318, 364, 395, 832, 848, 1027
BstMAI GTCTC 4 cut(s) 179, 184, 494, 533
BstMBI GATC 9 cut(s) 196, 220, 233, 315, 361, 392, 829, 845, 1024
BstMWI GCNNNNNNNGC 4 cut(s) 620, 683, 712, 1008
BstNI CCWGG 2 cut(s) 674, 869
BstSCI CCNGG 2 cut(s) 672, 867
BstUI CGCG 2 cut(s) 616, 1014
BstV2I GAAGAC 1 cut(s) 3
BstX2I RGATCY 2 cut(s) 233, 392
BstYI RGATCY 2 cut(s) 233, 392
BstZ17I GTATAC 1 cut(s) 383
Bsu36I CCTNAGG 1 cut(s) 161
BsuRI GGCC 1 cut(s) 715
BtgI CCRYGG 1 cut(s) 493
BtsCI GGATG 3 cut(s) 90, 690, 962
Cac8I GCNNGC 2 cut(s) 356, 599
CfoI GCGC 2 cut(s) 616, 1016
Cfr13I GGNCC 1 cut(s) 670
CseI GACGC 1 cut(s) 605
Csp6I GTAC 2 cut(s) 148, 438
CviAII CATG 7 cut(s) 359, 494, 572, 594, 743, 894, 961
CviQI GTAC 2 cut(s) 148, 438
DdeI CTNAG 5 cut(s) 101, 161, 240, 279, 535
DpnI GATC 9 cut(s) 198, 222, 235, 317, 363, 394, 831, 847, 1026
DpnII GATC 9 cut(s) 196, 220, 233, 315, 361, 392, 829, 845, 1024
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
Eco130I CCWWGG 1 cut(s) 493
Eco31I GGTCTC 2 cut(s) 184, 494
Eco47I GGWCC 1 cut(s) 670
Eco81I CCTNAGG 1 cut(s) 161
EcoRI GAATTC 1 cut(s) 769
EcoRII CCWGG 2 cut(s) 672, 867
EcoT14I CCWWGG 1 cut(s) 493
EcoT22I ATGCAT 2 cut(s) 343, 962
ErhI CCWWGG 1 cut(s) 493
FaeI CATG 7 cut(s) 362, 497, 575, 597, 746, 897, 964
FaqI GGGAC 1 cut(s) 436
FatI CATG 7 cut(s) 358, 493, 571, 593, 742, 893, 960
FauNDI CATATG 1 cut(s) 948
FbaI TGATCA 2 cut(s) 220, 361
FblI GTMKAC 1 cut(s) 382
Fnu4HI GCNGC 2 cut(s) 716, 1012
FokI GGATG 3 cut(s) 97, 677, 969
Fsp4HI GCNGC 2 cut(s) 716, 1012
FspBI CTAG 1 cut(s) 284
GlaI GCGC 2 cut(s) 615, 1015
GluI GCNGC 2 cut(s) 716, 1012
GsuI CTGGAG 2 cut(s) 418, 900
HaeIII GGCC 1 cut(s) 715
HapII CCGG 1 cut(s) 194
HgaI GACGC 1 cut(s) 605
HhaI GCGC 2 cut(s) 616, 1016
Hin1II CATG 7 cut(s) 362, 497, 575, 597, 746, 897, 964
Hin6I GCGC 2 cut(s) 614, 1014
HinP1I GCGC 2 cut(s) 614, 1014
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 4 cut(s) 24, 738, 854, 911
HpaII CCGG 1 cut(s) 194
HphI GGTGA 2 cut(s) 356, 887
Hpy166II GTNNAC 1 cut(s) 383
Hpy188III TCNNGA 2 cut(s) 122, 203
Hpy8I GTNNAC 1 cut(s) 383
HpyAV CCTTC 3 cut(s) 238, 866, 917
HpyCH4III ACNGT 3 cut(s) 59, 522, 1048
HpyCH4V TGCA 8 cut(s) 170, 341, 597, 623, 641, 706, 731, 960
HpyF10VI GCNNNNNNNGC 4 cut(s) 620, 683, 712, 1008
HpyF3I CTNAG 5 cut(s) 101, 161, 240, 279, 535
Hsp92II CATG 7 cut(s) 362, 497, 575, 597, 746, 897, 964
HspAI GCGC 2 cut(s) 614, 1014
Ksp22I TGATCA 2 cut(s) 220, 361
Kzo9I GATC 9 cut(s) 196, 220, 233, 315, 361, 392, 829, 845, 1024
LmnI GCTCC 4 cut(s) 162, 437, 805, 926
LweI GCATC 3 cut(s) 673, 718, 947
MaeI CTAG 1 cut(s) 284
MaeIII GTNAC 1 cut(s) 497
MalI GATC 9 cut(s) 198, 222, 235, 317, 363, 394, 831, 847, 1026
MboI GATC 9 cut(s) 196, 220, 233, 315, 361, 392, 829, 845, 1024
MboII GAAGA 7 cut(s) 3, 325, 575, 665, 668, 879, 882
MflI RGATCY 2 cut(s) 233, 392
MluCI AATT 5 cut(s) 260, 451, 552, 624, 769
MmeI TCCRAC 1 cut(s) 812
MnlI CCTC 9 cut(s) 25, 75, 247, 280, 326, 396, 559, 697, 705
Mph1103I ATGCAT 2 cut(s) 343, 962
MseI TTAA 5 cut(s) 293, 306, 509, 627, 981
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 2 cut(s) 674, 869
Mva1269I GAATGC 1 cut(s) 274
MvaI CCWGG 2 cut(s) 674, 869
MvnI CGCG 2 cut(s) 616, 1014
MwoI GCNNNNNNNGC 4 cut(s) 620, 683, 712, 1008
NcoI CCATGG 1 cut(s) 493
NdeI CATATG 1 cut(s) 948
NdeII GATC 9 cut(s) 196, 220, 233, 315, 361, 392, 829, 845, 1024
NlaIII CATG 7 cut(s) 362, 497, 575, 597, 746, 897, 964
NsiI ATGCAT 2 cut(s) 343, 962
NspV TTCGAA 1 cut(s) 767
PctI GAATGC 1 cut(s) 274
PfeI GAWTC 4 cut(s) 24, 738, 854, 911
PfoI TCCNGGA 1 cut(s) 867
PkrI GCNGC 2 cut(s) 717, 1013
Psp6I CCWGG 2 cut(s) 672, 867
PspGI CCWGG 2 cut(s) 672, 867
PspPI GGNCC 1 cut(s) 670
PsuI RGATCY 2 cut(s) 233, 392
RsaI GTAC 2 cut(s) 149, 439
RsaNI GTAC 2 cut(s) 148, 438
SaqAI TTAA 5 cut(s) 293, 306, 509, 627, 981
SatI GCNGC 2 cut(s) 716, 1012
Sau3AI GATC 9 cut(s) 196, 220, 233, 315, 361, 392, 829, 845, 1024
Sau96I GGNCC 1 cut(s) 670
ScaI AGTACT 1 cut(s) 439
ScrFI CCNGG 2 cut(s) 674, 869
SfaNI GCATC 3 cut(s) 673, 718, 947
SfuI TTCGAA 1 cut(s) 767
SinI GGWCC 1 cut(s) 670
Sse9I AATT 5 cut(s) 260, 451, 552, 624, 769
SsiI CCGC 2 cut(s) 716, 1012
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 2 cut(s) 672, 867
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 3 cut(s) 59, 522, 1048
TaqI TCGA 6 cut(s) 45, 51, 411, 767, 844, 1023
TasI AATT 5 cut(s) 260, 451, 552, 624, 769
TatI WGTACW 2 cut(s) 147, 437
TauI GCSGC 2 cut(s) 718, 1014
TfiI GAWTC 4 cut(s) 24, 738, 854, 911
Tru1I TTAA 5 cut(s) 293, 306, 509, 627, 981
Tru9I TTAA 5 cut(s) 293, 306, 509, 627, 981
TspDTI ATGAA 2 cut(s) 759, 882
VpaK11BI GGWCC 1 cut(s) 670
XapI RAATTY 3 cut(s) 451, 552, 769
XmiI GTMKAC 1 cut(s) 382
XspI CTAG 1 cut(s) 284
ZrmI AGTACT 1 cut(s) 439
Zsp2I ATGCAT 2 cut(s) 343, 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.