Rorug04G0001300

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002680
Physical Location & Seq
Forward (+)
301410 .. 304096
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0001300.1

Sequence Viewer

Length: 627 bp
ATGGAAGGGGATGGCGGTGGTGCGATGCCATTGTCAAGTGAGAATGCGAAAGAAGAGCAGCAGGGGACGTCCTACACTTATTGGGTGAGGCAAGTGACGGAGGAAGCGGCGCCTCCCCCGGTTCCCAGAATGCTCTCATCTCAGGATATTCTCTCCGCCCAGTCTCAGCCTGCCACCCTCGGCTCTCTCTGGAATCGGGCTGGAACTTGGGAGGAGAAGAATGTCAATAAATGGGCGACAGATAGAATAAAGGAGTTGCTTGTATCAGTGGGTTCCGTGGAGTTCCCAGGTGGCAAAGCACAAATATCAGATGTCTCCAAGTGTGTAGGGGATGCATTCTTGGTGACTGTGCGAAACAAGAAACGCGTTGGCTACACCTATGAACTAACCTTGAAAGTCAAAGGGGAATGGATTACCGGAGAGGAGAAAAAGATGGTAAAGGGCCAGATAGACATCCCAGAGTTTTCATATGGGGAACTCGATGACTTGCAGATGGAAGTGCGGTTGGGTGAAGAGAAGGATCTTCTGTATCAAGATAAGTTGCAAATTAGCCAAAATCTGAAGCTATTTTTGCAGCCTGTTCGTGAGAAATTACTTCAATTTGAACAGGAACTAAAAGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

208

Amino Acids

23.5

Weight (kDa)

5.0

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aha1_N PF09229 72 - 207 1e-29 Activator of Hsp90 ATPase, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 109
AccII CGCG 1 cut(s) 366
AciI CCGC 4 cut(s) 15, 107, 156, 502
AclWI GGATC 1 cut(s) 528
AcuI CTGAAG 1 cut(s) 581
AcyI GRCGYC 2 cut(s) 68, 110
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 3 cut(s) 394, 599, 605
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 1 cut(s) 565
AluI AGCT 1 cut(s) 565
Alw26I GTCTC 2 cut(s) 168, 319
AlwI GGATC 1 cut(s) 528
AoxI GGCC 1 cut(s) 442
ApeKI GCWGC 2 cut(s) 58, 574
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 442
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 3 cut(s) 97, 355, 521
BanI GGYRCC 1 cut(s) 109
BbvI GCAGC 2 cut(s) 70, 586
BccI CCATC 3 cut(s) 5, 427, 487
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BciT130I CCWGG 1 cut(s) 288
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 2 cut(s) 168, 319
BfoI RGCGCY 1 cut(s) 113
BisI GCNGC 3 cut(s) 59, 108, 575
BlsI GCNGC 3 cut(s) 60, 109, 576
Bme1390I CCNGG 2 cut(s) 119, 288
BmgT120I GGNCC 1 cut(s) 442
BmiI GGNNCC 3 cut(s) 111, 123, 274
BmrFI CCNGG 2 cut(s) 119, 288
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 2 cut(s) 15, 322
BmuI ACTGGG 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 119
BsaBI GATNNNNATC 1 cut(s) 452
BsaHI GRCGYC 2 cut(s) 68, 110
BsaJI CCNNGG 4 cut(s) 117, 178, 276, 286
BsaWI WCCGGW 1 cut(s) 416
Bse1I ACTGG 1 cut(s) 160
Bse8I GATNNNNATC 1 cut(s) 452
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 4 cut(s) 117, 178, 276, 286
BseGI GGATG 3 cut(s) 16, 337, 453
BseJI GATNNNNATC 1 cut(s) 452
BseMII CTCAG 2 cut(s) 155, 179
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 2 cut(s) 227, 437
BseXI GCAGC 2 cut(s) 70, 586
Bsh1236I CGCG 1 cut(s) 366
BshFI GGCC 1 cut(s) 444
BshNI GGYRCC 1 cut(s) 109
BsiSI CCGG 2 cut(s) 119, 417
BslFI GGGAC 1 cut(s) 79
BsmAI GTCTC 2 cut(s) 168, 319
BsmFI GGGAC 1 cut(s) 79
BsmI GAATGC 3 cut(s) 49, 135, 335
BsnI GGCC 1 cut(s) 444
Bsp143I GATC 1 cut(s) 520
BspACI CCGC 4 cut(s) 15, 107, 156, 502
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 2 cut(s) 154, 178
BspFNI CGCG 1 cut(s) 366
BspLI GGNNCC 3 cut(s) 111, 123, 274
BspPI GGATC 1 cut(s) 528
BspQI GCTCTTC 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 109
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 4 cut(s) 117, 178, 276, 286
BssMI GATC 1 cut(s) 520
BssNI GRCGYC 2 cut(s) 68, 110
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 1 cut(s) 349
Bst6I CTCTTC 2 cut(s) 48, 507
BstACI GRCGYC 2 cut(s) 68, 110
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 2 cut(s) 141, 165
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 3 cut(s) 16, 337, 453
BstFNI CGCG 1 cut(s) 366
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 1 cut(s) 523
BstMAI GTCTC 2 cut(s) 168, 319
BstMBI GATC 1 cut(s) 520
BstMWI GCNNNNNNNGC 1 cut(s) 571
BstNI CCWGG 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 117, 286
BstUI CGCG 1 cut(s) 366
BstV1I GCAGC 2 cut(s) 70, 586
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BsuRI GGCC 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 276
BtgZI GCGATG 1 cut(s) 38
BtsCI GGATG 3 cut(s) 16, 337, 453
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 1 cut(s) 171
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 1 cut(s) 442
CviJI RGCY 8 cut(s) 169, 183, 200, 372, 444, 552, 565, 577
CviKI_1 RGCY 8 cut(s) 169, 183, 200, 372, 444, 552, 565, 577
DdeI CTNAG 2 cut(s) 141, 165
DinI GGCGCC 1 cut(s) 111
DpnI GATC 1 cut(s) 522
DpnII GATC 1 cut(s) 520
Eam1104I CTCTTC 2 cut(s) 48, 507
EarI CTCTTC 2 cut(s) 48, 507
EciI GGCGGA 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 581
EcoRII CCWGG 1 cut(s) 286
EcoT22I ATGCAT 1 cut(s) 337
EgeI GGCGCC 1 cut(s) 111
EheI GGCGCC 1 cut(s) 111
FaiI YATR 3 cut(s) 381, 469, 471
FaqI GGGAC 1 cut(s) 79
FauNDI CATATG 1 cut(s) 469
Fnu4HI GCNGC 3 cut(s) 59, 108, 575
FokI GGATG 3 cut(s) 23, 344, 440
Fsp4HI GCNGC 3 cut(s) 59, 108, 575
GlaI GCGC 1 cut(s) 111
GluI GCNGC 3 cut(s) 59, 108, 575
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 1 cut(s) 444
HapII CCGG 2 cut(s) 119, 417
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 2 cut(s) 68, 110
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HinfI GANTC 1 cut(s) 193
HpaII CCGG 2 cut(s) 119, 417
HphI GGTGA 3 cut(s) 97, 355, 521
Hpy188I TCNGA 2 cut(s) 310, 561
Hpy188III TCNNGA 4 cut(s) 143, 190, 533, 584
HpyAV CCTTC 1 cut(s) 511
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 1 cut(s) 68
HpyCH4V TGCA 4 cut(s) 335, 490, 544, 574
HpyF10VI GCNNNNNNNGC 1 cut(s) 571
HpyF3I CTNAG 2 cut(s) 141, 165
HpySE526I ACGT 1 cut(s) 68
Hsp92I GRCGYC 2 cut(s) 68, 110
HspAI GCGC 1 cut(s) 110
KasI GGCGCC 1 cut(s) 109
Kzo9I GATC 1 cut(s) 520
LguI GCTCTTC 1 cut(s) 48
Lsp1109I GCAGC 2 cut(s) 70, 586
LweI GCATC 2 cut(s) 15, 322
MaeII ACGT 1 cut(s) 68
MaeIII GTNAC 2 cut(s) 94, 343
MalI GATC 1 cut(s) 522
MboI GATC 1 cut(s) 520
MboII GAAGA 4 cut(s) 65, 229, 515, 524
MflI RGATCY 1 cut(s) 520
MluCI AATT 3 cut(s) 546, 590, 599
MluI ACGCGT 1 cut(s) 364
Mly113I GGCGCC 1 cut(s) 110
MnlI CCTC 6 cut(s) 81, 94, 123, 188, 205, 415
Mph1103I ATGCAT 1 cut(s) 337
MspI CCGG 2 cut(s) 119, 417
MspR9I CCNGG 2 cut(s) 119, 288
Mva1269I GAATGC 3 cut(s) 49, 135, 335
MvaI CCWGG 1 cut(s) 288
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 1 cut(s) 571
NarI GGCGCC 1 cut(s) 110
NciI CCSGG 1 cut(s) 119
NdeI CATATG 1 cut(s) 469
NdeII GATC 1 cut(s) 520
NlaIV GGNNCC 3 cut(s) 111, 123, 274
NmeAIII GCCGAG 1 cut(s) 159
NmuCI GTSAC 2 cut(s) 94, 343
NsiI ATGCAT 1 cut(s) 337
PciSI GCTCTTC 1 cut(s) 48
PctI GAATGC 3 cut(s) 49, 135, 335
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 3 cut(s) 60, 109, 576
PluTI GGCGCC 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 286
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 3 cut(s) 111, 123, 274
PspPI GGNCC 1 cut(s) 442
PsuI RGATCY 1 cut(s) 520
SapI GCTCTTC 1 cut(s) 48
SatI GCNGC 3 cut(s) 59, 108, 575
Sau3AI GATC 1 cut(s) 520
Sau96I GGNCC 1 cut(s) 442
ScrFI CCNGG 2 cut(s) 119, 288
SetI ASST 5 cut(s) 71, 292, 380, 392, 567
SfaNI GCATC 2 cut(s) 15, 322
SfoI GGCGCC 1 cut(s) 111
Sse9I AATT 3 cut(s) 546, 590, 599
SsiI CCGC 4 cut(s) 15, 107, 156, 502
SspDI GGCGCC 1 cut(s) 109
StyD4I CCNGG 2 cut(s) 117, 286
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 1 cut(s) 71
TaqI TCGA 1 cut(s) 480
TasI AATT 3 cut(s) 546, 590, 599
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 1 cut(s) 193
TscAI CASTG 1 cut(s) 273
TseFI GTSAC 2 cut(s) 94, 343
TseI GCWGC 2 cut(s) 58, 574
Tsp45I GTSAC 2 cut(s) 94, 343
TspDTI ATGAA 2 cut(s) 396, 456
TspGWI ACGGA 2 cut(s) 113, 265
TspRI CASTG 1 cut(s) 273
ZraI GACGTC 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.