AT3G23280

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002680
Physical Location & Seq
Forward (+)
8321329 .. 8324477
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G23280.1

Sequence Viewer

Length: 1389 bp
ATGGGACAACAGCAATCAAAAGGGGAATTGCTGTATCAGCAAGTTAGTTATGGTAACTCTGAAGGAATTCGAGCTCTTCATCGAGATGGTGGAGACCTTGAGTGGATGGATAGAGAAGGAAAAACTCCGTTGATCTTGGCTTGTATGAACTCCGAGCTATTTGATGTTGCTAAGACTTTGATTGAGTTGGGTTCTAATGTTAATGCTTATCGTCCTGGTCGTCATGCTGGGACTCCTCTGCACCATGCTGCAAAAAGAGGTCTTGAGAACACTGTTAAGTTACTTCTTTCTCACGGTGCGAATCCACTTGTTCTTAATGATGATTGTCAGACACCACTTGAGGTCGCTAGAGTCAAAGGGTTTAGCAATGTTGTACGTGCAATTGAGAAACACATCTGCTTGTTCTCGGGTTGGATGCGTGAATTTTACGGCCCTACCTTTCTCGACTTATTTGCTCCGCAGCTTCTTTCAAGAAGAGTATGGGTAGTCATTGTACCTACTGGTTCAAGAAACCCTACAAAGCCTTTCAAGTTGGAGCTGGTTGTGTATGCTAGTCTGCAAGATGCACAGCCACGCACAGTGATGCCTTTGTGGAAAGCAAACTTGGAAGAACCAAAAGCGAAGCAGTCCGATACTTCAGTGATGATCGTTGATAACTCCACAATCCCAAGCCGCAGGATGAAGAAACGAAGGGTCTGCGCCTCCCACGGGAGACGTAGGCCTCAAGTAGTTAGGCAAACACGTCTTAAATTCGCACCTTCGACTGAAGGTGACAGCCAACAGCTAAAGTGGTTTTGTGATGCATGTAAAGGGATTCCACAGCCGATGCATCCACCGGTCTTCCTCCAAGCACCACCATCTGCTCCACCACCACCATCAGAAGATGGACTAGCCATGGGAATGAACGCCTCACTTCACACTACTATGAGTGACCCATCAAATCTCAATCACCATTCCATCGGTCAAGCAAGTTCCTCATCCGGTCCAAGCTCATCAACTGCACCTCCCTCAGGAAAAGCAAGTGCCTTTGGATTCAATAGCCATGGAATTGGCATTGTGCTAGAATCTTCACCATCAGCTCCTCCATTGACAGATGATGATATAGCTACAGTAGACGATGGTCCAATTCATTACCCATCAATCGATTCAACACCAGTCGATCTCCCATCTGCTGCTTCTCTTCCAGCTTCAACAGAAGGTGAAAGGAAAGAAGACGGGAACACTGGAACGTGTGCGATATGTCTCGATGCTCCATCTGAAGCAGTCTGTGTCCCGTGTGGACATGTCGCGGGATGTATGTCTTGCTTGAAGGAGATCAAATCCAAGAATTGGGGATGTCCCGTCTGTCGAGCCAAGATCGATCAGGTTATTAAGCTGTACCGTGTCTGA

Protein Analysis

462

Amino Acids

50.05

Weight (kDa)

8.33

Isoelectric Point (pI)

58.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_5 PF13857 60 - 116 3e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 78 - 128 2.2e-06 Ankyrin repeats (3 copies)
Ank PF00023 78 - 106 2.5e-06 Ankyrin repeat
zf-C3HC4_3 PF13920 409 - 456 1.3e-13 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 410 - 450 1e-06 Ring finger domain
Prok-RING_4 PF14447 411 - 455 1e-05 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1329
AccI GTMKAC 1 cut(s) 1113
AccII CGCG 1 cut(s) 1289
AciI CCGC 3 cut(s) 458, 673, 1289
AcsI RAATTY 3 cut(s) 66, 422, 749
AcuI CTGAAG 4 cut(s) 81, 621, 786, 1278
AfaI GTAC 3 cut(s) 375, 495, 1379
AfiI CCNNNNNNNGG 3 cut(s) 708, 1010, 1329
AflIII ACRYGT 3 cut(s) 740, 1229, 1282
AgeI ACCGGT 1 cut(s) 835
AgsI TTSAA 7 cut(s) 471, 507, 529, 1036, 1149, 1191, 1309
AjiI CACGTC 1 cut(s) 743
AjnI CCWGG 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
Alw21I GWGCWC 1 cut(s) 76
Alw26I GTCTC 3 cut(s) 87, 706, 1247
Ama87I CYCGRG 1 cut(s) 406
AoxI GGCC 2 cut(s) 430, 719
ApeKI GCWGC 3 cut(s) 248, 460, 1172
ApoI RAATTY 3 cut(s) 66, 422, 749
AsiGI ACCGGT 1 cut(s) 835
Asp700I GAANNNNTTC 1 cut(s) 66
AspLEI GCGC 1 cut(s) 701
AspS9I GGNCC 3 cut(s) 431, 983, 1121
AsuHPI GGTGA 4 cut(s) 782, 941, 1062, 1211
AvaI CYCGRG 1 cut(s) 406
AvaII GGWCC 2 cut(s) 983, 1121
AxyI CCTNAGG 1 cut(s) 1009
BaeI ACNNNNGTAYC 2 cut(s) 624, 657
BanII GRGCYC 1 cut(s) 76
BbsI GAAGAC 2 cut(s) 832, 1218
Bbv12I GWGCWC 1 cut(s) 76
BbvI GCAGC 3 cut(s) 235, 472, 1159
BceAI ACGGC 1 cut(s) 445
BcgI CGANNNNNNTGC 4 cut(s) 434, 468, 678, 712
BciT130I CCWGG 1 cut(s) 216
BcoDI GTCTC 3 cut(s) 87, 706, 1247
BfaI CTAG 4 cut(s) 348, 552, 890, 1061
BfmI CTRYAG 1 cut(s) 1107
BisI GCNGC 4 cut(s) 249, 461, 673, 1173
BlsI GCNGC 4 cut(s) 250, 462, 674, 1174
Bme1390I CCNGG 1 cut(s) 216
Bme18I GGWCC 2 cut(s) 983, 1121
BmeT110I CYCGRG 1 cut(s) 406
BmgBI CACGTC 1 cut(s) 743
BmgT120I GGNCC 3 cut(s) 431, 983, 1121
BmrFI CCNGG 1 cut(s) 216
BmsI GCATC 7 cut(s) 405, 553, 573, 790, 816, 838, 1237
BoxI GACNNNNGTC 1 cut(s) 1119
BpiI GAAGAC 2 cut(s) 832, 1218
BpuEI CTTGAG 4 cut(s) 119, 284, 359, 708
Bsa29I ATCGAT 2 cut(s) 1143, 1359
BsaAI YACGTR 1 cut(s) 377
BsaI GGTCTC 1 cut(s) 87
BsaJI CCNNGG 3 cut(s) 706, 894, 1042
BsaWI WCCGGW 2 cut(s) 835, 980
BsaXI ACNNNNNCTCC 2 cut(s) 988, 1018
Bsc4I CCNNNNNNNGG 3 cut(s) 708, 1010, 1329
Bse118I RCCGGY 1 cut(s) 835
Bse1I ACTGG 3 cut(s) 505, 1154, 1228
Bse21I CCTNAGG 1 cut(s) 1009
Bse3DI GCAATG 1 cut(s) 373
BseBI CCWGG 1 cut(s) 216
BseCI ATCGAT 2 cut(s) 1143, 1359
BseDI CCNNGG 3 cut(s) 706, 894, 1042
BseGI GGATG 7 cut(s) 111, 420, 684, 829, 977, 1298, 1340
BseLI CCNNNNNNNGG 3 cut(s) 708, 1010, 1329
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 1 cut(s) 1023
BseNI ACTGG 3 cut(s) 505, 1154, 1228
BseRI GAGGAG 2 cut(s) 225, 1071
BseXI GCAGC 3 cut(s) 235, 472, 1159
BseYI CCCAGC 1 cut(s) 227
BsgI GTGCAG 2 cut(s) 224, 984
Bsh1236I CGCG 1 cut(s) 1289
BshFI GGCC 2 cut(s) 432, 721
BshTI ACCGGT 1 cut(s) 835
BshVI ATCGAT 2 cut(s) 1143, 1359
BsiHKAI GWGCWC 1 cut(s) 76
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 2 cut(s) 836, 981
BslFI GGGAC 4 cut(s) 18, 244, 1256, 1323
BslI CCNNNNNNNGG 3 cut(s) 708, 1010, 1329
BsmAI GTCTC 3 cut(s) 87, 706, 1247
BsmBI CGTCTC 1 cut(s) 706
BsmFI GGGAC 4 cut(s) 18, 244, 1256, 1323
BsnI GGCC 2 cut(s) 432, 721
Bso31I GGTCTC 1 cut(s) 87
BsoBI CYCGRG 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 6 cut(s) 132, 645, 1159, 1314, 1356, 1360
Bsp19I CCATGG 2 cut(s) 894, 1042
BspACI CCGC 3 cut(s) 458, 673, 1289
BspANI GGCC 2 cut(s) 432, 721
BspCNI CTCAG 1 cut(s) 1022
BspDI ATCGAT 2 cut(s) 1143, 1359
BspFNI CGCG 1 cut(s) 1289
BspQI GCTCTTC 1 cut(s) 81
BspTNI GGTCTC 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 373
BsrFI RCCGGY 1 cut(s) 835
BsrI ACTGG 3 cut(s) 505, 1154, 1228
BssAI RCCGGY 1 cut(s) 835
BssECI CCNNGG 3 cut(s) 706, 894, 1042
BssMI GATC 6 cut(s) 132, 645, 1159, 1314, 1356, 1360
BssT1I CCWWGG 2 cut(s) 894, 1042
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 5 cut(s) 274, 296, 580, 1111, 1382
Bst6I CTCTTC 3 cut(s) 81, 469, 1185
BstBAI YACGTR 1 cut(s) 377
BstDEI CTNAG 2 cut(s) 171, 1009
BstDSI CCRYGG 3 cut(s) 706, 894, 1042
BstENI CCTNNNNNAGG 1 cut(s) 1008
BstF5I GGATG 7 cut(s) 111, 420, 684, 829, 977, 1298, 1340
BstFNI CGCG 1 cut(s) 1289
BstHHI GCGC 1 cut(s) 701
BstKTI GATC 6 cut(s) 135, 648, 1162, 1317, 1359, 1363
BstMAI GTCTC 3 cut(s) 87, 706, 1247
BstMBI GATC 6 cut(s) 132, 645, 1159, 1314, 1356, 1360
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 1 cut(s) 216
BstNSI RCATGY 2 cut(s) 807, 1286
BstPAI GACNNNNGTC 1 cut(s) 1119
BstSCI CCNGG 1 cut(s) 214
BstSFI CTRYAG 1 cut(s) 1107
BstUI CGCG 1 cut(s) 1289
BstV1I GCAGC 3 cut(s) 235, 472, 1159
BstV2I GAAGAC 2 cut(s) 832, 1218
BstXI CCANNNNNNTGG 1 cut(s) 1049
Bsu15I ATCGAT 2 cut(s) 1143, 1359
Bsu36I CCTNAGG 1 cut(s) 1009
BsuRI GGCC 2 cut(s) 432, 721
BsuTUI ATCGAT 2 cut(s) 1143, 1359
BtgI CCRYGG 3 cut(s) 706, 894, 1042
BtrI CACGTC 1 cut(s) 743
BtsCI GGATG 7 cut(s) 111, 420, 684, 829, 977, 1298, 1340
BtsIMutI CAGTG 4 cut(s) 270, 585, 645, 1221
CfoI GCGC 1 cut(s) 701
Cfr10I RCCGGY 1 cut(s) 835
Cfr13I GGNCC 3 cut(s) 431, 983, 1121
ClaI ATCGAT 2 cut(s) 1143, 1359
Csp6I GTAC 3 cut(s) 374, 494, 1378
CspAI ACCGGT 1 cut(s) 835
CviAII CATG 6 cut(s) 224, 245, 804, 895, 1043, 1283
CviQI GTAC 3 cut(s) 374, 494, 1378
DdeI CTNAG 2 cut(s) 171, 1009
DpnI GATC 6 cut(s) 134, 647, 1161, 1316, 1358, 1362
DpnII GATC 6 cut(s) 132, 645, 1159, 1314, 1356, 1360
Eam1104I CTCTTC 3 cut(s) 81, 469, 1185
EarI CTCTTC 3 cut(s) 81, 469, 1185
Ecl136II GAGCTC 1 cut(s) 74
Eco130I CCWWGG 2 cut(s) 894, 1042
Eco147I AGGCCT 1 cut(s) 721
Eco24I GRGCYC 1 cut(s) 76
Eco31I GGTCTC 1 cut(s) 87
Eco47I GGWCC 2 cut(s) 983, 1121
Eco53kI GAGCTC 1 cut(s) 74
Eco57I CTGAAG 4 cut(s) 81, 621, 786, 1278
Eco81I CCTNAGG 1 cut(s) 1009
Eco88I CYCGRG 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 74
EcoNI CCTNNNNNAGG 1 cut(s) 1008
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 214
EcoT14I CCWWGG 2 cut(s) 894, 1042
EcoT22I ATGCAT 2 cut(s) 805, 831
EcoT38I GRGCYC 1 cut(s) 76
ErhI CCWWGG 2 cut(s) 894, 1042
Esp3I CGTCTC 1 cut(s) 706
FaeI CATG 6 cut(s) 227, 248, 807, 898, 1046, 1286
FaqI GGGAC 4 cut(s) 18, 244, 1256, 1323
FatI CATG 6 cut(s) 223, 244, 803, 894, 1042, 1282
FauI CCCGC 1 cut(s) 1282
FblI GTMKAC 1 cut(s) 1113
Fnu4HI GCNGC 4 cut(s) 249, 461, 673, 1173
FokI GGATG 7 cut(s) 118, 427, 691, 816, 964, 1305, 1347
FriOI GRGCYC 1 cut(s) 76
Fsp4HI GCNGC 4 cut(s) 249, 461, 673, 1173
FspBI CTAG 4 cut(s) 348, 552, 890, 1061
GlaI GCGC 1 cut(s) 700
GluI GCNGC 4 cut(s) 249, 461, 673, 1173
GsaI CCCAGC 1 cut(s) 231
HaeIII GGCC 2 cut(s) 432, 721
HapII CCGG 2 cut(s) 836, 981
HhaI GCGC 1 cut(s) 701
Hin1II CATG 6 cut(s) 227, 248, 807, 898, 1046, 1286
Hin6I GCGC 1 cut(s) 699
HinP1I GCGC 1 cut(s) 699
HinfI GANTC 7 cut(s) 232, 301, 351, 814, 1032, 1064, 1145
HpaII CCGG 2 cut(s) 836, 981
HphI GGTGA 4 cut(s) 782, 941, 1062, 1211
Hpy166II GTNNAC 2 cut(s) 1114, 1280
Hpy188I TCNGA 7 cut(s) 61, 154, 330, 631, 880, 1258, 1388
Hpy188III TCNNGA 7 cut(s) 83, 263, 443, 471, 507, 1011, 1244
Hpy8I GTNNAC 2 cut(s) 1114, 1280
HpyAV CCTTC 7 cut(s) 56, 110, 684, 761, 768, 1190, 1303
HpyCH4III ACNGT 5 cut(s) 274, 296, 580, 1111, 1382
HpyCH4IV ACGT 4 cut(s) 376, 715, 742, 1229
HpyCH4V TGCA 8 cut(s) 241, 251, 380, 559, 566, 803, 829, 1001
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 2 cut(s) 171, 1009
HpySE526I ACGT 4 cut(s) 376, 715, 742, 1229
Hsp92II CATG 6 cut(s) 227, 248, 807, 898, 1046, 1286
HspAI GCGC 1 cut(s) 699
Kzo9I GATC 6 cut(s) 132, 645, 1159, 1314, 1356, 1360
LguI GCTCTTC 1 cut(s) 81
LmnI GCTCC 5 cut(s) 460, 535, 868, 1084, 1255
Lsp1109I GCAGC 3 cut(s) 235, 472, 1159
LweI GCATC 7 cut(s) 405, 553, 573, 790, 816, 838, 1237
MaeI CTAG 4 cut(s) 348, 552, 890, 1061
MaeII ACGT 4 cut(s) 376, 715, 742, 1229
MaeIII GTNAC 4 cut(s) 53, 279, 770, 929
MalI GATC 6 cut(s) 134, 647, 1161, 1316, 1358, 1362
MboI GATC 6 cut(s) 132, 645, 1159, 1314, 1356, 1360
MboII GAAGA 9 cut(s) 68, 486, 620, 694, 832, 893, 1059, 1172, 1223
MfeI CAATTG 1 cut(s) 381
MhlI GDGCHC 1 cut(s) 76
MluCI AATT 8 cut(s) 26, 66, 381, 422, 749, 1047, 1125, 1327
MlyI GAGTC 2 cut(s) 226, 360
MmeI TCCRAC 2 cut(s) 392, 513
Mph1103I ATGCAT 2 cut(s) 805, 831
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 5 cut(s) 201, 276, 315, 747, 1371
MslI CAYNNNNRTG 4 cut(s) 84, 581, 899, 923
MspI CCGG 2 cut(s) 836, 981
MspR9I CCNGG 1 cut(s) 216
MunI CAATTG 1 cut(s) 381
MvaI CCWGG 1 cut(s) 216
MvnI CGCG 1 cut(s) 1289
MwoI GCNNNNNNNGC 1 cut(s) 37
NcoI CCATGG 2 cut(s) 894, 1042
NdeII GATC 6 cut(s) 132, 645, 1159, 1314, 1356, 1360
NlaIII CATG 6 cut(s) 227, 248, 807, 898, 1046, 1286
NmuCI GTSAC 2 cut(s) 770, 929
NsiI ATGCAT 2 cut(s) 805, 831
NspI RCATGY 2 cut(s) 807, 1286
PceI AGGCCT 1 cut(s) 721
PciI ACATGT 1 cut(s) 1282
PciSI GCTCTTC 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 5 cut(s) 301, 814, 1032, 1064, 1145
PflMI CCANNNNNTGG 1 cut(s) 1329
PinAI ACCGGT 1 cut(s) 835
PkrI GCNGC 4 cut(s) 250, 462, 674, 1174
PleI GAGTC 2 cut(s) 226, 359
PpsI GAGTC 2 cut(s) 226, 359
Ppu21I YACGTR 1 cut(s) 377
PscI ACATGT 1 cut(s) 1282
PshAI GACNNNNGTC 1 cut(s) 1119
Psp124BI GAGCTC 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 214
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 1 cut(s) 214
PspPI GGNCC 3 cut(s) 431, 983, 1121
RsaI GTAC 3 cut(s) 375, 495, 1379
RsaNI GTAC 3 cut(s) 374, 494, 1378
RseI CAYNNNNRTG 4 cut(s) 84, 581, 899, 923
SacI GAGCTC 1 cut(s) 76
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 5 cut(s) 201, 276, 315, 747, 1371
SatI GCNGC 4 cut(s) 249, 461, 673, 1173
Sau3AI GATC 6 cut(s) 132, 645, 1159, 1314, 1356, 1360
Sau96I GGNCC 3 cut(s) 431, 983, 1121
SchI GAGTC 2 cut(s) 226, 360
ScrFI CCNGG 1 cut(s) 216
SduI GDGCHC 1 cut(s) 76
SfaNI GCATC 7 cut(s) 405, 553, 573, 790, 816, 838, 1237
SfcI CTRYAG 1 cut(s) 1107
SinI GGWCC 2 cut(s) 983, 1121
SmiMI CAYNNNNRTG 4 cut(s) 84, 581, 899, 923
SmlI CTYRAG 4 cut(s) 98, 263, 338, 723
SmoI CTYRAG 4 cut(s) 98, 263, 338, 723
Sse9I AATT 8 cut(s) 26, 66, 381, 422, 749, 1047, 1125, 1327
SseBI AGGCCT 1 cut(s) 721
SsiI CCGC 3 cut(s) 458, 673, 1289
SspMI CTAG 4 cut(s) 348, 552, 890, 1061
SstI GAGCTC 1 cut(s) 76
StuI AGGCCT 1 cut(s) 721
StyD4I CCNGG 1 cut(s) 214
StyI CCWWGG 2 cut(s) 894, 1042
TaaI ACNGT 5 cut(s) 274, 296, 580, 1111, 1382
TaiI ACGT 4 cut(s) 379, 718, 745, 1232
TaqI TCGA 9 cut(s) 70, 82, 444, 761, 1143, 1158, 1245, 1348, 1359
TaqII GACCGA 1 cut(s) 950
TasI AATT 8 cut(s) 26, 66, 381, 422, 749, 1047, 1125, 1327
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 5 cut(s) 301, 814, 1032, 1064, 1145
Tru1I TTAA 5 cut(s) 201, 276, 315, 747, 1371
Tru9I TTAA 5 cut(s) 201, 276, 315, 747, 1371
TscAI CASTG 4 cut(s) 277, 585, 645, 1228
TseFI GTSAC 2 cut(s) 770, 929
TseI GCWGC 3 cut(s) 248, 460, 1172
Tsp45I GTSAC 2 cut(s) 770, 929
TspDTI ATGAA 5 cut(s) 68, 161, 695, 917, 1118
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 4 cut(s) 277, 585, 645, 1228
Van91I CCANNNNNTGG 1 cut(s) 1329
VpaK11BI GGWCC 2 cut(s) 983, 1121
XagI CCTNNNNNAGG 1 cut(s) 1008
XapI RAATTY 3 cut(s) 66, 422, 749
XceI RCATGY 2 cut(s) 807, 1286
XmiI GTMKAC 1 cut(s) 1113
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 4 cut(s) 348, 552, 890, 1061
Zsp2I ATGCAT 2 cut(s) 805, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.