MD13G1224400.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009928
Physical Location & Seq
Forward (+)
21958723 .. 21959920
1198 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1224400.v1.1.491

Sequence Viewer

Length: 798 bp
ATGTATTTGGGAATGATGGCAGGTCACCATATGGGTTGGGGTTTGATAGAAGAGGAGGGTTGGAGAAAGGGGCCTTGGACTGCTGAGGAAGATAGGTTGCTTATTGAGTATGTGAGGGTTCATGGTGAAGGCAGATGGAACTCTGTCGCTAGGCTTGCAGGACTGAAGAGAAATGGAAAGAGCTGCAGATTGAGGTGGGTGAATTATTTGAGGCCAGACCTTAAGAGGGGGCAAATTACTCCACATGAAGAGAGAATCATTGTAGACCTTCATGCAAGATGGGGAAATAGATGGTCAACAATTGCAAGGAGTTTGCCCGGAAGGACTGACAACGAAATCAAGAACTACTGGAGGACCCATTTCAAGAAAAAGGCAGTGAAAGCGCCTTCTGATGCCTCCGAGAAAGCGAAAAATCGCCTTTTAAGGAGGCAACAGTTTCACCAGCAGCAGCAACAGTTGCAACAGATTAATCAACCAGATGTCAAGAAAATCATGGCCTTACTGGATGAAAATGACAACAATAAAATATCATCCTGCTGGCCTCAAGTGAAGCCGGATTCGGAGACCTCTACTACTACCACTGTATACCCCAACACAGCTGATCAGGAGCAAGGATTCTTCTGCTCCATGTTGCATGGCAATGTCTATATGCCTGAACCTGCCTCCACTAATGAGGACAATTATCTGTGGGATGGTTTGTGGAACTTGGATGATATCCATGGTAATTTCAACACAGCTTCAGCTTGTTCAACAGGCAAACCTAGTCTGCTCACCAACTTGGTCGTTCCCTTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

266

Amino Acids

30.69

Weight (kDa)

8.97

Isoelectric Point (pI)

57.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 23 - 70 5.6e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 26 - 84 3.3e-13 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 76 - 120 6.8e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 80 - 134 1.1e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 11, 667
AccI GTMKAC 2 cut(s) 264, 585
AcuI CTGAAG 2 cut(s) 185, 723
AfiI CCNNNNNNNGG 1 cut(s) 226
AflII CTTAAG 1 cut(s) 221
AgsI TTSAA 3 cut(s) 364, 730, 750
AjuI GAANNNNNNNTTGG 2 cut(s) 58, 90
AluBI AGCT 4 cut(s) 183, 599, 737, 743
AluI AGCT 4 cut(s) 183, 599, 737, 743
Alw26I GTCTC 1 cut(s) 557
AoxI GGCC 4 cut(s) 71, 212, 495, 539
ApeKI GCWGC 3 cut(s) 183, 445, 448
AseI ATTAAT 1 cut(s) 468
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 2 cut(s) 71, 354
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 5 cut(s) 17, 137, 211, 431, 763
AvaII GGWCC 1 cut(s) 354
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 3 cut(s) 170, 457, 460
BccI CCATC 5 cut(s) 10, 129, 273, 285, 686
BclI TGATCA 1 cut(s) 601
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 557
BfaI CTAG 2 cut(s) 150, 762
BfmI CTRYAG 1 cut(s) 184
BfoI RGCGCY 1 cut(s) 386
BfrI CTTAAG 1 cut(s) 221
BfuAI ACCTGC 2 cut(s) 11, 667
BisI GCNGC 3 cut(s) 184, 446, 449
BlsI GCNGC 3 cut(s) 185, 447, 450
Bme1390I CCNGG 1 cut(s) 318
Bme18I GGWCC 1 cut(s) 354
BmgT120I GGNCC 2 cut(s) 71, 354
BmiI GGNNCC 2 cut(s) 72, 356
BmrFI CCNGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 382
BpmI CTGGAG 1 cut(s) 370
Bpu10I CCTNAGC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 528
BpuMI CCSGG 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 2 cut(s) 74, 718
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse1I ACTGG 2 cut(s) 353, 507
Bse3DI GCAATG 1 cut(s) 646
BseDI CCNNGG 2 cut(s) 74, 718
BseGI GGATG 4 cut(s) 511, 530, 697, 715
BseLI CCNNNNNNNGG 1 cut(s) 226
BseMI GCAATG 1 cut(s) 646
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 2 cut(s) 353, 507
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 3 cut(s) 170, 457, 460
BshFI GGCC 4 cut(s) 73, 214, 497, 541
BsiSI CCGG 2 cut(s) 318, 554
BslI CCNNNNNNNGG 1 cut(s) 226
BsmAI GTCTC 1 cut(s) 557
BsnI GGCC 4 cut(s) 73, 214, 497, 541
Bso31I GGTCTC 1 cut(s) 557
Bsp143I GATC 1 cut(s) 601
Bsp19I CCATGG 1 cut(s) 718
BspANI GGCC 4 cut(s) 73, 214, 497, 541
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 2 cut(s) 72, 356
BspMAI CTGCAG 1 cut(s) 188
BspMI ACCTGC 2 cut(s) 11, 667
BspTI CTTAAG 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 646
BsrI ACTGG 2 cut(s) 353, 507
BssECI CCNNGG 2 cut(s) 74, 718
BssMI GATC 1 cut(s) 601
BssNAI GTATAC 1 cut(s) 586
BssT1I CCWWGG 2 cut(s) 74, 718
Bst1107I GTATAC 1 cut(s) 586
Bst4CI ACNGT 3 cut(s) 435, 456, 583
Bst6I CTCTTC 3 cut(s) 45, 161, 243
BstAFI CTTAAG 1 cut(s) 221
BstAPI GCANNNNNTGC 1 cut(s) 457
BstC8I GCNNGC 2 cut(s) 156, 539
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 718
BstEII GGTNACC 1 cut(s) 23
BstF5I GGATG 4 cut(s) 511, 530, 697, 715
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 1 cut(s) 604
BstMAI GTCTC 1 cut(s) 557
BstMBI GATC 1 cut(s) 601
BstMWI GCNNNNNNNGC 3 cut(s) 155, 380, 457
BstPI GGTNACC 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 316
BstSFI CTRYAG 1 cut(s) 184
BstV1I GCAGC 3 cut(s) 170, 457, 460
BstZ17I GTATAC 1 cut(s) 586
BsuRI GGCC 4 cut(s) 73, 214, 497, 541
BtgI CCRYGG 1 cut(s) 718
BtsCI GGATG 4 cut(s) 511, 530, 697, 715
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 2 cut(s) 381, 579
BveI ACCTGC 2 cut(s) 11, 667
Cac8I GCNNGC 2 cut(s) 156, 539
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 71, 354
CviAII CATG 7 cut(s) 122, 245, 272, 493, 628, 635, 719
DdeI CTNAG 1 cut(s) 84
DpnI GATC 1 cut(s) 603
DpnII GATC 1 cut(s) 601
Eam1104I CTCTTC 3 cut(s) 45, 161, 243
EarI CTCTTC 3 cut(s) 45, 161, 243
Eco130I CCWWGG 2 cut(s) 74, 718
Eco31I GGTCTC 1 cut(s) 557
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 1 cut(s) 354
Eco57I CTGAAG 2 cut(s) 185, 723
Eco91I GGTNACC 1 cut(s) 23
EcoO109I RGGNCCY 2 cut(s) 71, 354
EcoO65I GGTNACC 1 cut(s) 23
EcoRV GATATC 1 cut(s) 715
EcoT14I CCWWGG 2 cut(s) 74, 718
ErhI CCWWGG 2 cut(s) 74, 718
FaeI CATG 7 cut(s) 125, 248, 275, 496, 631, 638, 722
FatI CATG 7 cut(s) 121, 244, 271, 492, 627, 634, 718
FauNDI CATATG 1 cut(s) 30
FbaI TGATCA 1 cut(s) 601
FblI GTMKAC 2 cut(s) 264, 585
Fnu4HI GCNGC 3 cut(s) 184, 446, 449
FokI GGATG 4 cut(s) 517, 518, 704, 722
Fsp4HI GCNGC 3 cut(s) 184, 446, 449
FspBI CTAG 2 cut(s) 150, 762
GlaI GCGC 1 cut(s) 384
GluI GCNGC 3 cut(s) 184, 446, 449
GsuI CTGGAG 1 cut(s) 370
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 4 cut(s) 73, 214, 497, 541
HapII CCGG 2 cut(s) 318, 554
HhaI GCGC 1 cut(s) 385
Hin1II CATG 7 cut(s) 125, 248, 275, 496, 631, 638, 722
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HinfI GANTC 3 cut(s) 255, 557, 615
HpaII CCGG 2 cut(s) 318, 554
HphI GGTGA 5 cut(s) 17, 137, 211, 431, 763
Hpy166II GTNNAC 3 cut(s) 265, 297, 586
Hpy188I TCNGA 3 cut(s) 391, 400, 562
Hpy188III TCNNGA 4 cut(s) 340, 364, 484, 605
Hpy8I GTNNAC 3 cut(s) 265, 297, 586
HpyAV CCTTC 4 cut(s) 122, 278, 315, 396
HpyCH4III ACNGT 3 cut(s) 435, 456, 583
HpyCH4V TGCA 6 cut(s) 158, 186, 275, 305, 460, 634
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 380, 457
HpyF3I CTNAG 1 cut(s) 84
Hsp92II CATG 7 cut(s) 125, 248, 275, 496, 631, 638, 722
HspAI GCGC 1 cut(s) 383
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 1 cut(s) 601
LmnI GCTCC 2 cut(s) 607, 629
Lsp1109I GCAGC 3 cut(s) 170, 457, 460
LweI GCATC 1 cut(s) 382
MaeI CTAG 2 cut(s) 150, 762
MaeIII GTNAC 1 cut(s) 23
MalI GATC 1 cut(s) 603
MboI GATC 1 cut(s) 601
MboII GAAGA 5 cut(s) 62, 101, 178, 260, 610
MfeI CAATTG 1 cut(s) 300
MluCI AATT 5 cut(s) 202, 234, 300, 679, 724
MmeI TCCRAC 1 cut(s) 41
MseI TTAA 3 cut(s) 222, 422, 468
MslI CAYNNNNRTG 1 cut(s) 639
MspA1I CMGCKG 1 cut(s) 599
MspCI CTTAAG 1 cut(s) 221
MspI CCGG 2 cut(s) 318, 554
MspR9I CCNGG 1 cut(s) 318
MunI CAATTG 1 cut(s) 300
MwoI GCNNNNNNNGC 3 cut(s) 155, 380, 457
NciI CCSGG 1 cut(s) 318
NcoI CCATGG 1 cut(s) 718
NdeI CATATG 1 cut(s) 30
NdeII GATC 1 cut(s) 601
NlaIII CATG 7 cut(s) 125, 248, 275, 496, 631, 638, 722
NlaIV GGNNCC 2 cut(s) 72, 356
NmuCI GTSAC 1 cut(s) 23
PfeI GAWTC 3 cut(s) 255, 557, 615
PkrI GCNGC 3 cut(s) 185, 447, 450
PpuMI RGGWCCY 1 cut(s) 354
PshBI ATTAAT 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 354
PspEI GGTNACC 1 cut(s) 23
PspN4I GGNNCC 2 cut(s) 72, 356
PspPI GGNCC 2 cut(s) 71, 354
PspPPI RGGWCCY 1 cut(s) 354
PstI CTGCAG 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 599
RseI CAYNNNNRTG 1 cut(s) 639
SaqAI TTAA 3 cut(s) 222, 422, 468
SatI GCNGC 3 cut(s) 184, 446, 449
Sau3AI GATC 1 cut(s) 601
Sau96I GGNCC 2 cut(s) 71, 354
ScrFI CCNGG 1 cut(s) 318
SfaNI GCATC 1 cut(s) 382
SfcI CTRYAG 1 cut(s) 184
SinI GGWCC 1 cut(s) 354
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 2 cut(s) 221, 543
SmoI CTYRAG 2 cut(s) 221, 543
Sse9I AATT 5 cut(s) 202, 234, 300, 679, 724
SspMI CTAG 2 cut(s) 150, 762
StyD4I CCNGG 1 cut(s) 316
StyI CCWWGG 2 cut(s) 74, 718
TaaI ACNGT 3 cut(s) 435, 456, 583
TasI AATT 5 cut(s) 202, 234, 300, 679, 724
TfiI GAWTC 3 cut(s) 255, 557, 615
Tru1I TTAA 3 cut(s) 222, 422, 468
Tru9I TTAA 3 cut(s) 222, 422, 468
TscAI CASTG 2 cut(s) 381, 586
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 3 cut(s) 183, 445, 448
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 4 cut(s) 110, 260, 261, 522
TspRI CASTG 2 cut(s) 381, 586
Vha464I CTTAAG 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 354
VspI ATTAAT 1 cut(s) 468
XmiI GTMKAC 2 cut(s) 264, 585
XspI CTAG 2 cut(s) 150, 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.