MD16G1228600.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009928
Physical Location & Seq
Forward (+)
23353345 .. 23354653
1309 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1228600.v1.1.491

Sequence Viewer

Length: 720 bp
ATGTATTTGGGGATGATGGCAGGAGGACATCATAGGGGTTGGGGAATGATAGAAGATGAGGCATGGAGAAAGGGACCTTGGACTGCTGAGGAAGATGGATTGCTCATTGAGCATGTGAGGCTTCATGGTGAAGGCAGATGGAACTCTCTGGCTAGGCTTGCAGGGCTGAAAAGGAATGGAAAGAGCTGCAGATTGAGGTGGGTGAACTATTTGAGGCCAGAACTTAAGAGGGGGCACATAACTCCTCATGAAGAAAGCATCATTCTAGATCTACATGCTAGATGGGGAAACAGGTGGTCGACAATTGCACGAAGTTTGCCTGGAAGGACTGACAATGAGATCAAGAACTACTGGAGGACTCATTTCAAGAAAAGGGTAAAAGCGCCTTCTGATGCCTCCGAAGGGGCCAAAACCCGCCTCTTAAGGAGACAAAAATCCCATAATCAGCAGCAACAACAACAAAATTTGCAAGTGGATCAAGAAGTGAAAAGAATCATGTCCTTATTGGACGAAAATGAGACTAAACTGCCGTTATATTGGCCTCACGCAGCTGATCATCATCAGGAGCAAGGCTTCTTCTATTCCATGCTCGATGGCAATATTGTCAATGTGTCTTTGCCTGAAGCTGCCTCCAGCAATGAGGACAATTTTCTGTGGGATGGTTTGTGGAACTTAAATGATGTACATGCAAACTTTATTAACACCACCAGGCAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

240

Amino Acids

27.92

Weight (kDa)

9.21

Isoelectric Point (pI)

63.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 24 - 71 2e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 27 - 85 7.5e-11 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 77 - 121 1.2e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 81 - 126 2.6e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 299
AciI CCGC 1 cut(s) 415
AclWI GGATC 1 cut(s) 483
AcsI RAATTY 1 cut(s) 463
AcuI CTGAAG 1 cut(s) 642
AfaI GTAC 1 cut(s) 684
AfiI CCNNNNNNNGG 1 cut(s) 402
AflII CTTAAG 2 cut(s) 224, 421
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 2 cut(s) 319, 707
AjuI GAANNNNNNNTTGG 2 cut(s) 61, 93
AluBI AGCT 4 cut(s) 186, 551, 626, 717
AluI AGCT 4 cut(s) 186, 551, 626, 717
Alw26I GTCTC 2 cut(s) 421, 512
AlwI GGATC 1 cut(s) 483
AoxI GGCC 3 cut(s) 215, 405, 539
ApeKI GCWGC 4 cut(s) 186, 448, 548, 626
ApoI RAATTY 1 cut(s) 463
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 2 cut(s) 74, 405
AsuHPI GGTGA 2 cut(s) 140, 214
AvaII GGWCC 1 cut(s) 74
BaeGI GKGCMC 1 cut(s) 237
BbvCI CCTCAGC 1 cut(s) 87
BbvI GCAGC 4 cut(s) 173, 460, 560, 613
BccI CCATC 6 cut(s) 10, 89, 132, 276, 587, 653
BceAI ACGGC 1 cut(s) 514
BciT130I CCWGG 2 cut(s) 321, 709
BclI TGATCA 1 cut(s) 553
BcoDI GTCTC 2 cut(s) 421, 512
BfaI CTAG 4 cut(s) 153, 266, 279, 718
BfmI CTRYAG 1 cut(s) 187
BfoI RGCGCY 1 cut(s) 386
BfrI CTTAAG 2 cut(s) 224, 421
BglII AGATCT 1 cut(s) 268
BisI GCNGC 4 cut(s) 187, 449, 549, 627
BlsI GCNGC 4 cut(s) 188, 450, 550, 628
Bme1390I CCNGG 2 cut(s) 321, 709
Bme18I GGWCC 1 cut(s) 74
BmgT120I GGNCC 2 cut(s) 74, 405
BmiI GGNNCC 2 cut(s) 75, 406
BmrFI CCNGG 2 cut(s) 321, 709
BmsI GCATC 2 cut(s) 267, 382
BpmI CTGGAG 2 cut(s) 373, 616
Bpu10I CCTNAGC 1 cut(s) 87
BsaBI GATNNNNATC 1 cut(s) 558
BsaJI CCNNGG 1 cut(s) 77
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse1I ACTGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 643
Bse8I GATNNNNATC 1 cut(s) 558
BseBI CCWGG 2 cut(s) 321, 709
BseDI CCNNGG 1 cut(s) 77
BseGI GGATG 2 cut(s) 18, 664
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 1 cut(s) 402
BseMI GCAATG 1 cut(s) 643
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 1 cut(s) 356
BseRI GAGGAG 1 cut(s) 234
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 4 cut(s) 173, 460, 560, 613
BshFI GGCC 3 cut(s) 217, 407, 541
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 402
BsmAI GTCTC 2 cut(s) 421, 512
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 3 cut(s) 217, 407, 541
Bsp1286I GDGCHC 1 cut(s) 237
Bsp1407I TGTACA 1 cut(s) 682
Bsp143I GATC 4 cut(s) 268, 339, 475, 553
BspACI CCGC 1 cut(s) 415
BspANI GGCC 3 cut(s) 217, 407, 541
BspCNI CTCAG 1 cut(s) 79
BspHI TCATGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 75, 406
BspMAI CTGCAG 1 cut(s) 191
BspPI GGATC 1 cut(s) 483
BspTI CTTAAG 2 cut(s) 224, 421
BsrDI GCAATG 1 cut(s) 643
BsrGI TGTACA 1 cut(s) 682
BsrI ACTGG 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 77
BssMI GATC 4 cut(s) 268, 339, 475, 553
BssT1I CCWWGG 1 cut(s) 77
Bst2UI CCWGG 2 cut(s) 321, 709
BstAFI CTTAAG 2 cut(s) 224, 421
BstAUI TGTACA 1 cut(s) 682
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 18, 664
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 4 cut(s) 271, 342, 478, 556
BstMAI GTCTC 2 cut(s) 421, 512
BstMBI GATC 4 cut(s) 268, 339, 475, 553
BstMWI GCNNNNNNNGC 4 cut(s) 109, 118, 158, 163
BstNI CCWGG 2 cut(s) 321, 709
BstNSI RCATGY 3 cut(s) 116, 278, 689
BstSCI CCNGG 2 cut(s) 319, 707
BstSFI CTRYAG 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 4 cut(s) 173, 460, 560, 613
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BsuRI GGCC 3 cut(s) 217, 407, 541
BtsCI GGATG 2 cut(s) 18, 664
Cac8I GCNNGC 1 cut(s) 159
CciI TCATGA 1 cut(s) 247
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 74, 405
Csp6I GTAC 1 cut(s) 683
CviAII CATG 8 cut(s) 63, 113, 125, 248, 275, 496, 586, 686
CviQI GTAC 1 cut(s) 683
DdeI CTNAG 1 cut(s) 87
DpnI GATC 4 cut(s) 270, 341, 477, 555
DpnII GATC 4 cut(s) 268, 339, 475, 553
Eco130I CCWWGG 1 cut(s) 77
Eco47I GGWCC 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 642
EcoO109I RGGNCCY 1 cut(s) 74
EcoRII CCWGG 2 cut(s) 319, 707
EcoT14I CCWWGG 1 cut(s) 77
ErhI CCWWGG 1 cut(s) 77
FaeI CATG 8 cut(s) 66, 116, 128, 251, 278, 499, 589, 689
FaqI GGGAC 1 cut(s) 87
FatI CATG 8 cut(s) 62, 112, 124, 247, 274, 495, 585, 685
FauI CCCGC 1 cut(s) 422
FbaI TGATCA 1 cut(s) 553
FblI GTMKAC 1 cut(s) 299
Fnu4HI GCNGC 4 cut(s) 187, 449, 549, 627
FokI GGATG 2 cut(s) 25, 671
Fsp4HI GCNGC 4 cut(s) 187, 449, 549, 627
FspBI CTAG 4 cut(s) 153, 266, 279, 718
GlaI GCGC 1 cut(s) 384
GluI GCNGC 4 cut(s) 187, 449, 549, 627
GsuI CTGGAG 2 cut(s) 373, 616
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 3 cut(s) 217, 407, 541
HhaI GCGC 1 cut(s) 385
Hin1II CATG 8 cut(s) 66, 116, 128, 251, 278, 499, 589, 689
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HinfI GANTC 2 cut(s) 358, 492
HphI GGTGA 2 cut(s) 140, 214
Hpy166II GTNNAC 2 cut(s) 205, 300
Hpy188I TCNGA 2 cut(s) 391, 400
Hpy188III TCNNGA 6 cut(s) 248, 266, 343, 367, 479, 563
Hpy8I GTNNAC 2 cut(s) 205, 300
HpyAV CCTTC 4 cut(s) 125, 318, 395, 396
HpyCH4V TGCA 5 cut(s) 161, 189, 308, 469, 689
HpyF10VI GCNNNNNNNGC 4 cut(s) 109, 118, 158, 163
HpyF3I CTNAG 1 cut(s) 87
Hsp92II CATG 8 cut(s) 66, 116, 128, 251, 278, 499, 589, 689
HspAI GCGC 1 cut(s) 383
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 4 cut(s) 268, 339, 475, 553
LmnI GCTCC 1 cut(s) 565
Lsp1109I GCAGC 4 cut(s) 173, 460, 560, 613
LweI GCATC 2 cut(s) 267, 382
MaeI CTAG 4 cut(s) 153, 266, 279, 718
MalI GATC 4 cut(s) 270, 341, 477, 555
MboI GATC 4 cut(s) 268, 339, 475, 553
MboII GAAGA 4 cut(s) 65, 104, 263, 568
MfeI CAATTG 1 cut(s) 303
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 3 cut(s) 303, 463, 646
MlyI GAGTC 1 cut(s) 352
MseI TTAA 4 cut(s) 225, 422, 674, 699
MspA1I CMGCKG 1 cut(s) 551
MspCI CTTAAG 2 cut(s) 224, 421
MspR9I CCNGG 2 cut(s) 321, 709
MunI CAATTG 1 cut(s) 303
MvaI CCWGG 2 cut(s) 321, 709
MwoI GCNNNNNNNGC 4 cut(s) 109, 118, 158, 163
NdeII GATC 4 cut(s) 268, 339, 475, 553
NlaIII CATG 8 cut(s) 66, 116, 128, 251, 278, 499, 589, 689
NlaIV GGNNCC 2 cut(s) 75, 406
NspI RCATGY 3 cut(s) 116, 278, 689
PagI TCATGA 1 cut(s) 247
PfeI GAWTC 1 cut(s) 492
PkrI GCNGC 4 cut(s) 188, 450, 550, 628
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
PpuMI RGGWCCY 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 74
Psp6I CCWGG 2 cut(s) 319, 707
PspGI CCWGG 2 cut(s) 319, 707
PspN4I GGNNCC 2 cut(s) 75, 406
PspPI GGNCC 2 cut(s) 74, 405
PspPPI RGGWCCY 1 cut(s) 74
PstI CTGCAG 1 cut(s) 191
PsuI RGATCY 1 cut(s) 268
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 1 cut(s) 684
RsaNI GTAC 1 cut(s) 683
SalI GTCGAC 1 cut(s) 298
SaqAI TTAA 4 cut(s) 225, 422, 674, 699
SatI GCNGC 4 cut(s) 187, 449, 549, 627
Sau3AI GATC 4 cut(s) 268, 339, 475, 553
Sau96I GGNCC 2 cut(s) 74, 405
SchI GAGTC 1 cut(s) 352
ScrFI CCNGG 2 cut(s) 321, 709
SduI GDGCHC 1 cut(s) 237
SetI ASST 7 cut(s) 79, 188, 200, 296, 553, 628, 719
SfaNI GCATC 2 cut(s) 267, 382
SfcI CTRYAG 1 cut(s) 187
SinI GGWCC 1 cut(s) 74
SmlI CTYRAG 2 cut(s) 224, 421
SmoI CTYRAG 2 cut(s) 224, 421
Sse9I AATT 3 cut(s) 303, 463, 646
SsiI CCGC 1 cut(s) 415
SspI AATATT 1 cut(s) 601
SspMI CTAG 4 cut(s) 153, 266, 279, 718
StyD4I CCNGG 2 cut(s) 319, 707
StyI CCWWGG 1 cut(s) 77
TaqI TCGA 2 cut(s) 299, 591
TasI AATT 3 cut(s) 303, 463, 646
TatI WGTACW 1 cut(s) 682
TfiI GAWTC 1 cut(s) 492
Tru1I TTAA 4 cut(s) 225, 422, 674, 699
Tru9I TTAA 4 cut(s) 225, 422, 674, 699
TseI GCWGC 4 cut(s) 186, 448, 548, 626
TspDTI ATGAA 2 cut(s) 113, 264
Vha464I CTTAAG 2 cut(s) 224, 421
VpaK11BI GGWCC 1 cut(s) 74
XapI RAATTY 1 cut(s) 463
XbaI TCTAGA 1 cut(s) 265
XceI RCATGY 3 cut(s) 116, 278, 689
XmiI GTMKAC 1 cut(s) 299
XspI CTAG 4 cut(s) 153, 266, 279, 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.