Rw4G005220
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009928
Physical Location & Seq
Forward (+)
10625857 .. 10627056
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G005220.1

Sequence Viewer

Length: 801 bp
ATGTATTGTGGGATGATGGCAGCTCATATGGGTTGGGGAATAATAGAAGAAGAGGGTTGGAGAAAGGGTCCTTGGACTTCTGAGGAAGATAGATTGCTTATTGAGTATGTAAGGCTTCATGGTGAAGGAAGATGGAACTCTGTGGCTAGGCTTGCAGGACTGAAAAGAAATGGAAAGAGCTGCAGACTGAGGTGGGTGAATTATCTGAGGCCAGACCTTAAGAGAGGGCAGATAACTCCTCATGAAGAAAGCATAATTCTAGAGCTACATGCTAGGTGGGGGAACAGGTGGTCAACGATTGCAAGAAGCTTGCCTGGGAGGACTGATAATGAGATCAAGAACTACTGGAGGACCCATTTCAAGAAAAAGGCCAAAGTGCCTTCTGATGCCTCTGAGAAGGCCAAAACTCGCCTCTTACGGCGGCAACAGTTTCATCATCAACAGCAGCAGCAGCAACAATTGCAGCTGATTAATCAAGCAGACATGAAAAGAATCATGGCCTTGCTGGATGAAAATGACACCAAAATATCGTCGTGGCCACCTCAAGCAGCCCAAGGTAAGCAGGACGTGGACACTAGTACTATAGCATACCCTAATCACACAATTACTGAGGAGCAAGGCTTCTTCTATTCTATGCTCAATGGTAATGTGCCTCAAGTTGCTGAAGCTTCTTCCAGCGAGGACAGTTTTCTGTGGGATGGCTTGTGGAACTTGGATGATGTCCATGTCAATTTTACCACTTCAGCTTGTGCAACAGGCAAAGCTAGTACTTTTCACAACTTTGTGGTTCCCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

266

Amino Acids

30.74

Weight (kDa)

8.84

Isoelectric Point (pI)

60.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 22 - 69 2.5e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 25 - 83 1.3e-13 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 75 - 119 1e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 79 - 125 1.8e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 421
AcoI YGGCCR 1 cut(s) 536
AcuI CTGAAG 2 cut(s) 684, 726
AfaI GTAC 2 cut(s) 580, 769
AflII CTTAAG 1 cut(s) 218
AgsI TTSAA 1 cut(s) 361
AhlI ACTAGT 1 cut(s) 575
AjiI CACGTC 1 cut(s) 568
AjnI CCWGG 1 cut(s) 313
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AluBI AGCT 8 cut(s) 23, 180, 265, 309, 466, 668, 746, 764
AluI AGCT 8 cut(s) 23, 180, 265, 309, 466, 668, 746, 764
AoxI GGCC 5 cut(s) 209, 369, 399, 498, 536
ApeKI GCWGC 7 cut(s) 20, 180, 445, 448, 451, 463, 548
AseI ATTAAT 1 cut(s) 471
AspS9I GGNCC 2 cut(s) 68, 351
AsuHPI GGTGA 2 cut(s) 134, 208
AvaII GGWCC 2 cut(s) 68, 351
BalI TGGCCA 1 cut(s) 538
BbvI GCAGC 7 cut(s) 32, 167, 457, 460, 463, 475, 560
BccI CCATC 3 cut(s) 10, 126, 692
BceAI ACGGC 1 cut(s) 434
BciT130I CCWGG 1 cut(s) 315
BcuI ACTAGT 1 cut(s) 575
BfaI CTAG 5 cut(s) 147, 260, 273, 576, 765
BfmI CTRYAG 2 cut(s) 181, 582
BfrI CTTAAG 1 cut(s) 218
BisI GCNGC 8 cut(s) 21, 181, 422, 446, 449, 452, 464, 549
BlsI GCNGC 8 cut(s) 22, 182, 423, 447, 450, 453, 465, 550
BmcAI AGTACT 2 cut(s) 580, 769
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 2 cut(s) 68, 351
BmgBI CACGTC 1 cut(s) 568
BmgT120I GGNCC 2 cut(s) 68, 351
BmiI GGNNCC 3 cut(s) 69, 353, 789
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 376
BpmI CTGGAG 1 cut(s) 367
BpuEI CTTGAG 2 cut(s) 528, 639
BsaJI CCNNGG 3 cut(s) 71, 314, 553
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bse1I ACTGG 1 cut(s) 350
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 3 cut(s) 71, 314, 553
BseGI GGATG 4 cut(s) 18, 514, 703, 721
BseMII CTCAG 5 cut(s) 72, 179, 197, 384, 600
BseNI ACTGG 1 cut(s) 350
BseRI GAGGAG 2 cut(s) 228, 626
BseXI GCAGC 7 cut(s) 32, 167, 457, 460, 463, 475, 560
BshFI GGCC 5 cut(s) 211, 371, 401, 500, 538
BsnI GGCC 5 cut(s) 211, 371, 401, 500, 538
Bsp143I GATC 1 cut(s) 333
BspACI CCGC 1 cut(s) 421
BspANI GGCC 5 cut(s) 211, 371, 401, 500, 538
BspCNI CTCAG 5 cut(s) 73, 180, 198, 385, 601
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 3 cut(s) 69, 353, 789
BspMAI CTGCAG 1 cut(s) 185
BspTI CTTAAG 1 cut(s) 218
BsrI ACTGG 1 cut(s) 350
BssECI CCNNGG 3 cut(s) 71, 314, 553
BssMI GATC 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 71, 553
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 2 cut(s) 429, 686
Bst6I CTCTTC 1 cut(s) 45
BstAFI CTTAAG 1 cut(s) 218
BstAPI GCANNNNNTGC 1 cut(s) 460
BstC8I GCNNGC 2 cut(s) 153, 311
BstDEI CTNAG 5 cut(s) 81, 188, 206, 393, 609
BstF5I GGATG 4 cut(s) 18, 514, 703, 721
BstKTI GATC 1 cut(s) 336
BstMBI GATC 1 cut(s) 333
BstMWI GCNNNNNNNGC 3 cut(s) 152, 451, 460
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 2 cut(s) 181, 582
BstV1I GCAGC 7 cut(s) 32, 167, 457, 460, 463, 475, 560
BsuRI GGCC 5 cut(s) 211, 371, 401, 500, 538
BtrI CACGTC 1 cut(s) 568
BtsCI GGATG 4 cut(s) 18, 514, 703, 721
Cac8I GCNNGC 2 cut(s) 153, 311
CciI TCATGA 1 cut(s) 241
Cfr13I GGNCC 2 cut(s) 68, 351
Csp6I GTAC 2 cut(s) 579, 768
CviAII CATG 6 cut(s) 119, 242, 269, 484, 496, 725
CviQI GTAC 2 cut(s) 579, 768
DdeI CTNAG 5 cut(s) 81, 188, 206, 393, 609
DpnI GATC 1 cut(s) 335
DpnII GATC 1 cut(s) 333
EaeI YGGCCR 1 cut(s) 536
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco130I CCWWGG 2 cut(s) 71, 553
Eco47I GGWCC 2 cut(s) 68, 351
Eco57I CTGAAG 2 cut(s) 684, 726
EcoO109I RGGNCCY 2 cut(s) 68, 351
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 2 cut(s) 71, 553
ErhI CCWWGG 2 cut(s) 71, 553
FaeI CATG 6 cut(s) 122, 245, 272, 487, 499, 728
FatI CATG 6 cut(s) 118, 241, 268, 483, 495, 724
FauNDI CATATG 1 cut(s) 27
Fnu4HI GCNGC 8 cut(s) 21, 181, 422, 446, 449, 452, 464, 549
FokI GGATG 4 cut(s) 25, 521, 710, 728
Fsp4HI GCNGC 8 cut(s) 21, 181, 422, 446, 449, 452, 464, 549
FspBI CTAG 5 cut(s) 147, 260, 273, 576, 765
GluI GCNGC 8 cut(s) 21, 181, 422, 446, 449, 452, 464, 549
GsuI CTGGAG 1 cut(s) 367
HaeIII GGCC 5 cut(s) 211, 371, 401, 500, 538
Hin1II CATG 6 cut(s) 122, 245, 272, 487, 499, 728
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HindIII AAGCTT 2 cut(s) 307, 666
HinfI GANTC 1 cut(s) 492
HphI GGTGA 2 cut(s) 134, 208
Hpy166II GTNNAC 2 cut(s) 294, 571
Hpy188I TCNGA 4 cut(s) 82, 207, 385, 394
Hpy188III TCNNGA 4 cut(s) 242, 260, 337, 361
Hpy8I GTNNAC 2 cut(s) 294, 571
Hpy99I CGWCG 1 cut(s) 535
HpyAV CCTTC 3 cut(s) 119, 390, 391
HpyCH4III ACNGT 2 cut(s) 429, 686
HpyCH4IV ACGT 1 cut(s) 567
HpyCH4V TGCA 5 cut(s) 155, 183, 302, 463, 752
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 451, 460
HpyF3I CTNAG 5 cut(s) 81, 188, 206, 393, 609
HpySE526I ACGT 1 cut(s) 567
Hsp92II CATG 6 cut(s) 122, 245, 272, 487, 499, 728
Kzo9I GATC 1 cut(s) 333
LmnI GCTCC 1 cut(s) 613
Lsp1109I GCAGC 7 cut(s) 32, 167, 457, 460, 463, 475, 560
LweI GCATC 1 cut(s) 376
MaeI CTAG 5 cut(s) 147, 260, 273, 576, 765
MaeII ACGT 1 cut(s) 567
MalI GATC 1 cut(s) 335
MboI GATC 1 cut(s) 333
MboII GAAGA 7 cut(s) 59, 62, 98, 141, 257, 616, 663
MfeI CAATTG 1 cut(s) 458
MlsI TGGCCA 1 cut(s) 538
MluCI AATT 5 cut(s) 199, 255, 458, 603, 730
MluNI TGGCCA 1 cut(s) 538
MmeI TCCRAC 1 cut(s) 38
Mox20I TGGCCA 1 cut(s) 538
MscI TGGCCA 1 cut(s) 538
MseI TTAA 2 cut(s) 219, 471
Msp20I TGGCCA 1 cut(s) 538
MspA1I CMGCKG 1 cut(s) 466
MspCI CTTAAG 1 cut(s) 218
MspR9I CCNGG 1 cut(s) 315
MunI CAATTG 1 cut(s) 458
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 3 cut(s) 152, 451, 460
NdeI CATATG 1 cut(s) 27
NdeII GATC 1 cut(s) 333
NlaIII CATG 6 cut(s) 122, 245, 272, 487, 499, 728
NlaIV GGNNCC 3 cut(s) 69, 353, 789
NspI RCATGY 1 cut(s) 272
PagI TCATGA 1 cut(s) 241
PfeI GAWTC 1 cut(s) 492
PkrI GCNGC 8 cut(s) 22, 182, 423, 447, 450, 453, 465, 550
PpuMI RGGWCCY 2 cut(s) 68, 351
PshBI ATTAAT 1 cut(s) 471
Psp5II RGGWCCY 2 cut(s) 68, 351
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 69, 353, 789
PspPI GGNCC 2 cut(s) 68, 351
PspPPI RGGWCCY 2 cut(s) 68, 351
PstI CTGCAG 1 cut(s) 185
PvuII CAGCTG 1 cut(s) 466
RsaI GTAC 2 cut(s) 580, 769
RsaNI GTAC 2 cut(s) 579, 768
SaqAI TTAA 2 cut(s) 219, 471
SatI GCNGC 8 cut(s) 21, 181, 422, 446, 449, 452, 464, 549
Sau3AI GATC 1 cut(s) 333
Sau96I GGNCC 2 cut(s) 68, 351
ScaI AGTACT 2 cut(s) 580, 769
ScrFI CCNGG 1 cut(s) 315
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 2 cut(s) 181, 582
SinI GGWCC 2 cut(s) 68, 351
SmlI CTYRAG 3 cut(s) 218, 543, 654
SmoI CTYRAG 3 cut(s) 218, 543, 654
SpeI ACTAGT 1 cut(s) 575
Sse9I AATT 5 cut(s) 199, 255, 458, 603, 730
SsiI CCGC 1 cut(s) 421
SspMI CTAG 5 cut(s) 147, 260, 273, 576, 765
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 2 cut(s) 71, 553
TaaI ACNGT 2 cut(s) 429, 686
TaiI ACGT 1 cut(s) 570
TasI AATT 5 cut(s) 199, 255, 458, 603, 730
TatI WGTACW 2 cut(s) 578, 767
TauI GCSGC 1 cut(s) 424
TfiI GAWTC 1 cut(s) 492
Tru1I TTAA 2 cut(s) 219, 471
Tru9I TTAA 2 cut(s) 219, 471
TseI GCWGC 7 cut(s) 20, 180, 445, 448, 451, 463, 548
TspDTI ATGAA 5 cut(s) 107, 258, 422, 500, 525
Vha464I CTTAAG 1 cut(s) 218
VpaK11BI GGWCC 2 cut(s) 68, 351
VspI ATTAAT 1 cut(s) 471
XbaI TCTAGA 1 cut(s) 259
XceI RCATGY 1 cut(s) 272
XspI CTAG 5 cut(s) 147, 260, 273, 576, 765
ZrmI AGTACT 2 cut(s) 580, 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.