Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004895

Physical Location & Seq

Genomic Coordinates Forward (+)

31000233 .. 31006361

Gene Structure 6129 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1258500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGCGCAGGTGGCTCTGTTGTACTTGTCACGTCGAAGAGTCTTATCCATCAAACGAAAATGAACACCTGAAGAGCCCAAAGCACTATGCAGATGGGAACCAAAAGGGATCTAAGGTGTCAGCTCCTGTAAAATCTGAAGTACAGAAGGCTGCTCCACCTATTGAAGTGCCTCAATTATCTGTGGAGGAGCTGAAAGAGAAGACTGACAATTTTGGATCCAAATCATTGATCGGTGAAGGTTCGTATGGGAGAGTGTATTATGCAAGCTTGAATGATGGTAAAGCTGTGGCCGTGAAGAAGCTTGATGTTGCCTCTGAACCTGAGACAAATGTTGAGTTTTTGACCCAGGTTTCCATGGTTTCAAGACTGAAGCACGAAAATCTTGTTGAGTTGCTTGGTTACTGCGTTGATGGAAGTCTGCGTGTTTTGGCTTATGAGTTTGCAACCATGGGATCTCTACATGACATATTACATGGTAGAAAGGGAGTTCAAGGGGCTCAACCTGGCCCTACACTAGGCTGGATGCAGCGGGTAAGAATTGCAGTTGATGCAGCTAGGGGGTTGGAATACTTACACGAGAAGGTTCAACCTGCTATCATACACAGAGATATAAGATCTAGCAATGTGCTTCTCTTTGAAGATTTTAAAGCCAAGATAGCAGATTTTAATCTGTCAAATCAGGCTCCTGATATGGCTGCTCGTCTTCATTCTACTCGAGTTTTGGGAACATTTGGTTATCATGCCCCAGAGTATGCAATGACTGGTCAATTGACACAGAAGAGTGATGTGTACAGCTTTGGAGTGGTTCTACTAGAACTACTGACTGGGAGGAAACCTGTTGATCATACAATGCCTCGTGGACAGCAAAGTCTTGTTACTTGGGCTACTCCAAGATTGAGCGAAGACAAAGTTAAACAATGTGTTGATCCAAAGCTAAAGGAGTATCCTCCTAAGGGGGTCGCTAAGCTGGCAGCTGTTGCAGCATTGTGTGTGCAATATGAAGCTGAGTTCCGGCCAAATATGAGCATTGTTGTGAAGGCTCTTCAACCGCTTCTGAAGCCACCAGCAACGGCTCCGGAGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0030054 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

362

Amino Acids

39.88

Weight (kDa)

8.38

Isoelectric Point (pI)

33.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	74 - 286	4.5e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	75 - 348	2.8e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004895)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43230 AT2G43230 AT3G59350 AT3G59350 AT3G59350 AT3G59350 AT3G59350 AT3G59350
fragaria_vesca	FvH4_4g11280 FvH4_4g11280 FvH4_4g11280
malus_domestica	MD04G1038500.v1.1 MD13G1258500.v1.1
prunus_persica	Prupe.1G189000_v2.0.a1 Prupe.1G189000_v2.0.a1
pyrus_communis	pycom13g25750
rosa_chinensis	RchiOBHm_Chr4g0407431
rosa_laevigata	RLG00000008720
rosa_multiflora	Rmu_sc0004841.1_g000042
rosa_roxburghii	Rroxscaffold_5G00352020
rosa_rugosa	Rorug04G0079700 Rorug04G0079700 Rorug04G0079800 Rorug04G0079900
rosa_samantha	Rh4AG144300 Rh4BG142200 Rh4CG150900 Rh4DG138300
rosa_wichuraiana	Rw0G014480 Rw4G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	867
Acc16I	TGCGCA	1 cut(s)	5
Acc36I	ACCTGC	1 cut(s)	598
AccIII	TCCGGA	1 cut(s)	1075
AciI	CCGC	2 cut(s)	529, 1049
AclWI	GGATC	5 cut(s)	115, 210, 223, 460, 920
AcoI	YGGCCR	2 cut(s)	288, 1013
AcuI	CTGAAG	4 cut(s)	89, 156, 389, 1076
AfaI	GTAC	3 cut(s)	22, 141, 791
AfiI	CCNNNNNNNGG	2 cut(s)	515, 953
AgsI	TTSAA	7 cut(s)	164, 271, 363, 491, 587, 638, 1046
AjiI	CACGTC	1 cut(s)	31
AjnI	CCWGG	2 cut(s)	345, 502
Alw26I	GTCTC	1 cut(s)	317
AlwI	GGATC	5 cut(s)	115, 210, 223, 460, 920
AlwNI	CAGNNNCTG	1 cut(s)	125
Ama87I	CYCGRG	1 cut(s)	714
Aor13HI	TCCGGA	1 cut(s)	1075
AoxI	GGCC	3 cut(s)	288, 505, 1013
ApeKI	GCWGC	6 cut(s)	149, 526, 551, 695, 971, 980
AspLEI	GCGC	1 cut(s)	6
AspS9I	GGNCC	1 cut(s)	506
AsuHPI	GGTGA	1 cut(s)	245
AvaI	CYCGRG	1 cut(s)	714
AxyI	CCTNAGG	1 cut(s)	951
BamHI	GGATCC	1 cut(s)	215
BanII	GRGCYC	2 cut(s)	77, 499
BauI	CACGAG	2 cut(s)	575, 855
BbsI	GAAGAC	3 cut(s)	206, 695, 909
BbvI	GCAGC	6 cut(s)	136, 538, 563, 682, 983, 992
BccI	CCATC	4 cut(s)	55, 86, 269, 404
BceAI	ACGGC	1 cut(s)	275
BciT130I	CCWGG	2 cut(s)	347, 504
BciVI	GTATCC	1 cut(s)	954
BclI	TGATCA	1 cut(s)	841
BcoDI	GTCTC	1 cut(s)	317
BfaI	CTAG	4 cut(s)	515, 555, 618, 812
BfuAI	ACCTGC	1 cut(s)	598
BfuI	GTATCC	1 cut(s)	954
BglII	AGATCT	1 cut(s)	614
BisI	GCNGC	6 cut(s)	150, 527, 552, 696, 972, 981
BlpI	GCTNAGC	1 cut(s)	963
BlsI	GCNGC	6 cut(s)	151, 528, 553, 697, 973, 982
Bme1390I	CCNGG	2 cut(s)	347, 504
BmeT110I	CYCGRG	1 cut(s)	714
BmgBI	CACGTC	1 cut(s)	31
BmgT120I	GGNCC	1 cut(s)	506
BmiI	GGNNCC	4 cut(s)	98, 217, 684, 1074
BmrFI	CCNGG	2 cut(s)	347, 504
BmrI	ACTGGG	1 cut(s)	834
BmsI	GCATC	2 cut(s)	513, 538
BmuI	ACTGGG	1 cut(s)	834
BpiI	GAAGAC	3 cut(s)	206, 695, 909
Bpu1102I	GCTNAGC	1 cut(s)	963
BsaBI	GATNNNNATC	2 cut(s)	220, 666
BsaJI	CCNNGG	3 cut(s)	345, 354, 447
BsaWI	WCCGGW	1 cut(s)	1075
Bsc4I	CCNNNNNNNGG	2 cut(s)	515, 953
Bse1I	ACTGG	2 cut(s)	766, 829
Bse21I	CCTNAGG	1 cut(s)	951
Bse3DI	GCAATG	2 cut(s)	628, 762
Bse8I	GATNNNNATC	2 cut(s)	220, 666
BseAI	TCCGGA	1 cut(s)	1075
BseBI	CCWGG	2 cut(s)	347, 504
BseDI	CCNNGG	3 cut(s)	345, 354, 447
BseGI	GGATG	1 cut(s)	528
BseJI	GATNNNNATC	2 cut(s)	220, 666
BseLI	CCNNNNNNNGG	2 cut(s)	515, 953
BseMI	GCAATG	2 cut(s)	628, 762
BseMII	CTCAG	2 cut(s)	312, 996
BseNI	ACTGG	2 cut(s)	766, 829
BseRI	GAGGAG	1 cut(s)	200
BseXI	GCAGC	6 cut(s)	136, 538, 563, 682, 983, 992
BshFI	GGCC	3 cut(s)	290, 507, 1015
BsiHKCI	CYCGRG	1 cut(s)	714
BsiSI	CCGG	2 cut(s)	1012, 1076
BslI	CCNNNNNNNGG	2 cut(s)	515, 953
BsmAI	GTCTC	1 cut(s)	317
BsnI	GGCC	3 cut(s)	290, 507, 1015
BsoBI	CYCGRG	1 cut(s)	714
Bsp1286I	GDGCHC	2 cut(s)	77, 499
Bsp13I	TCCGGA	1 cut(s)	1075
Bsp1407I	TGTACA	1 cut(s)	789
Bsp143I	GATC	7 cut(s)	107, 215, 228, 452, 614, 841, 925
Bsp1720I	GCTNAGC	1 cut(s)	963
Bsp19I	CCATGG	2 cut(s)	354, 447
BspACI	CCGC	2 cut(s)	529, 1049
BspANI	GGCC	3 cut(s)	290, 507, 1015
BspCNI	CTCAG	2 cut(s)	313, 997
BspEI	TCCGGA	1 cut(s)	1075
BspLI	GGNNCC	4 cut(s)	98, 217, 684, 1074
BspMI	ACCTGC	1 cut(s)	598
BspPI	GGATC	5 cut(s)	115, 210, 223, 460, 920
BspQI	GCTCTTC	2 cut(s)	65, 1047
BsrDI	GCAATG	2 cut(s)	628, 762
BsrGI	TGTACA	1 cut(s)	789
BsrI	ACTGG	2 cut(s)	766, 829
BssECI	CCNNGG	3 cut(s)	345, 354, 447
BssMI	GATC	7 cut(s)	107, 215, 228, 452, 614, 841, 925
BssSI	CACGAG	2 cut(s)	575, 855
BssT1I	CCWWGG	2 cut(s)	354, 447
Bst2BI	CACGAG	2 cut(s)	575, 855
Bst2UI	CCWGG	2 cut(s)	347, 504
Bst6I	CTCTTC	4 cut(s)	30, 65, 773, 1047
BstAPI	GCANNNNNTGC	2 cut(s)	548, 977
BstAUI	TGTACA	1 cut(s)	789
BstC8I	GCNNGC	2 cut(s)	265, 969
BstDEI	CTNAG	5 cut(s)	111, 321, 951, 963, 1005
BstDSI	CCRYGG	2 cut(s)	354, 447
BstENI	CCTNNNNNAGG	1 cut(s)	513
BstF5I	GGATG	1 cut(s)	528
BstHHI	GCGC	1 cut(s)	6
BstKTI	GATC	7 cut(s)	110, 218, 231, 455, 617, 844, 928
BstMAI	GTCTC	1 cut(s)	317
BstMBI	GATC	7 cut(s)	107, 215, 228, 452, 614, 841, 925
BstMWI	GCNNNNNNNGC	7 cut(s)	10, 548, 656, 968, 977, 980, 1057
BstNI	CCWGG	2 cut(s)	347, 504
BstSCI	CCNGG	2 cut(s)	345, 502
BstV1I	GCAGC	6 cut(s)	136, 538, 563, 682, 983, 992
BstV2I	GAAGAC	3 cut(s)	206, 695, 909
BstX2I	RGATCY	4 cut(s)	107, 215, 452, 614
BstYI	RGATCY	4 cut(s)	107, 215, 452, 614
Bsu36I	CCTNAGG	1 cut(s)	951
BsuI	GTATCC	1 cut(s)	954
BsuRI	GGCC	3 cut(s)	290, 507, 1015
BtgI	CCRYGG	2 cut(s)	354, 447
BtrI	CACGTC	1 cut(s)	31
BtsCI	GGATG	1 cut(s)	528
BveI	ACCTGC	1 cut(s)	598
Cac8I	GCNNGC	2 cut(s)	265, 969
CaiI	CAGNNNCTG	1 cut(s)	125
CfoI	GCGC	1 cut(s)	6
Cfr13I	GGNCC	1 cut(s)	506
Csp6I	GTAC	3 cut(s)	21, 140, 790
CviAII	CATG	5 cut(s)	355, 448, 461, 473, 740
CviQI	GTAC	3 cut(s)	21, 140, 790
DdeI	CTNAG	5 cut(s)	111, 321, 951, 963, 1005
DpnI	GATC	7 cut(s)	109, 217, 230, 454, 616, 843, 927
DpnII	GATC	7 cut(s)	107, 215, 228, 452, 614, 841, 925
DraI	TTTAAA	1 cut(s)	646
DrdI	GACNNNNNNGTC	1 cut(s)	867
DseDI	GACNNNNNNGTC	1 cut(s)	867
EaeI	YGGCCR	2 cut(s)	288, 1013
Eam1104I	CTCTTC	4 cut(s)	30, 65, 773, 1047
EarI	CTCTTC	4 cut(s)	30, 65, 773, 1047
Eco130I	CCWWGG	2 cut(s)	354, 447
Eco24I	GRGCYC	2 cut(s)	77, 499
Eco57I	CTGAAG	4 cut(s)	89, 156, 389, 1076
Eco81I	CCTNAGG	1 cut(s)	951
Eco88I	CYCGRG	1 cut(s)	714
EcoNI	CCTNNNNNAGG	1 cut(s)	513
EcoRII	CCWGG	2 cut(s)	345, 502
EcoT14I	CCWWGG	2 cut(s)	354, 447
EcoT38I	GRGCYC	2 cut(s)	77, 499
ErhI	CCWWGG	2 cut(s)	354, 447
FaeI	CATG	5 cut(s)	358, 451, 464, 476, 743
FatI	CATG	5 cut(s)	354, 447, 460, 472, 739
FauI	CCCGC	1 cut(s)	522
FbaI	TGATCA	1 cut(s)	841
Fnu4HI	GCNGC	6 cut(s)	150, 527, 552, 696, 972, 981
FokI	GGATG	1 cut(s)	535
FriOI	GRGCYC	2 cut(s)	77, 499
Fsp4HI	GCNGC	6 cut(s)	150, 527, 552, 696, 972, 981
FspBI	CTAG	4 cut(s)	515, 555, 618, 812
FspI	TGCGCA	1 cut(s)	5
GlaI	GCGC	1 cut(s)	5
GluI	GCNGC	6 cut(s)	150, 527, 552, 696, 972, 981
HaeIII	GGCC	3 cut(s)	290, 507, 1015
HapII	CCGG	2 cut(s)	1012, 1076
HhaI	GCGC	1 cut(s)	6
Hin1II	CATG	5 cut(s)	358, 451, 464, 476, 743
Hin6I	GCGC	1 cut(s)	4
HinP1I	GCGC	1 cut(s)	4
HindIII	AAGCTT	2 cut(s)	265, 299
HinfI	GANTC	2 cut(s)	38, 1079
HpaII	CCGG	2 cut(s)	1012, 1076
HphI	GGTGA	1 cut(s)	245
Hpy166II	GTNNAC	2 cut(s)	790, 860
Hpy188I	TCNGA	3 cut(s)	136, 316, 1056
Hpy188III	TCNNGA	4 cut(s)	363, 686, 1076, 1083
Hpy8I	GTNNAC	2 cut(s)	790, 860
Hpy99I	CGWCG	1 cut(s)	35
HpyAV	CCTTC	4 cut(s)	139, 230, 574, 1030
HpyCH4IV	ACGT	1 cut(s)	30
HpyCH4V	TGCA	9 cut(s)	89, 263, 443, 526, 542, 551, 755, 980, 994
HpyF10VI	GCNNNNNNNGC	7 cut(s)	10, 548, 656, 968, 977, 980, 1057
HpyF3I	CTNAG	5 cut(s)	111, 321, 951, 963, 1005
HpySE526I	ACGT	1 cut(s)	30
Hsp92II	CATG	5 cut(s)	358, 451, 464, 476, 743
HspAI	GCGC	1 cut(s)	4
Kpn2I	TCCGGA	1 cut(s)	1075
Ksp22I	TGATCA	1 cut(s)	841
Kzo9I	GATC	7 cut(s)	107, 215, 228, 452, 614, 841, 925
LguI	GCTCTTC	2 cut(s)	65, 1047
LmnI	GCTCC	5 cut(s)	127, 157, 187, 688, 1078
Lsp1109I	GCAGC	6 cut(s)	136, 538, 563, 682, 983, 992
LweI	GCATC	2 cut(s)	513, 538
MaeI	CTAG	4 cut(s)	515, 555, 618, 812
MaeII	ACGT	1 cut(s)	30
MaeIII	GTNAC	3 cut(s)	26, 398, 874
MalI	GATC	7 cut(s)	109, 217, 230, 454, 616, 843, 927
MboI	GATC	7 cut(s)	107, 215, 228, 452, 614, 841, 925
MboII	GAAGA	9 cut(s)	47, 82, 211, 307, 650, 695, 790, 914, 1034
MfeI	CAATTG	1 cut(s)	767
MflI	RGATCY	4 cut(s)	107, 215, 452, 614
MhlI	GDGCHC	2 cut(s)	77, 499
MluCI	AATT	4 cut(s)	173, 208, 537, 767
MlyI	GAGTC	1 cut(s)	47
MmeI	TCCRAC	1 cut(s)	543
MnlI	CCTC	6 cut(s)	178, 180, 322, 822, 864, 957
MroI	TCCGGA	1 cut(s)	1075
MseI	TTAA	3 cut(s)	645, 666, 912
MslI	CAYNNNNRTG	1 cut(s)	1031
MspA1I	CMGCKG	2 cut(s)	529, 974
MspI	CCGG	2 cut(s)	1012, 1076
MspR9I	CCNGG	2 cut(s)	347, 504
MunI	CAATTG	1 cut(s)	767
MvaI	CCWGG	2 cut(s)	347, 504
MwoI	GCNNNNNNNGC	7 cut(s)	10, 548, 656, 968, 977, 980, 1057
NcoI	CCATGG	2 cut(s)	354, 447
NdeII	GATC	7 cut(s)	107, 215, 228, 452, 614, 841, 925
NlaIII	CATG	5 cut(s)	358, 451, 464, 476, 743
NlaIV	GGNNCC	4 cut(s)	98, 217, 684, 1074
NmuCI	GTSAC	1 cut(s)	26
NsbI	TGCGCA	1 cut(s)	5
PaeR7I	CTCGAG	1 cut(s)	714
PciSI	GCTCTTC	2 cut(s)	65, 1047
PkrI	GCNGC	6 cut(s)	151, 528, 553, 697, 973, 982
PleI	GAGTC	1 cut(s)	46
PpsI	GAGTC	1 cut(s)	46
Psp6I	CCWGG	2 cut(s)	345, 502
PspGI	CCWGG	2 cut(s)	345, 502
PspN4I	GGNNCC	4 cut(s)	98, 217, 684, 1074
PspPI	GGNCC	1 cut(s)	506
PspXI	VCTCGAGB	1 cut(s)	714
PstNI	CAGNNNCTG	1 cut(s)	125
PsuI	RGATCY	4 cut(s)	107, 215, 452, 614
PvuII	CAGCTG	1 cut(s)	974
RsaI	GTAC	3 cut(s)	22, 141, 791
RsaNI	GTAC	3 cut(s)	21, 140, 790
RseI	CAYNNNNRTG	1 cut(s)	1031
SapI	GCTCTTC	2 cut(s)	65, 1047
SaqAI	TTAA	3 cut(s)	645, 666, 912
SatI	GCNGC	6 cut(s)	150, 527, 552, 696, 972, 981
Sau3AI	GATC	7 cut(s)	107, 215, 228, 452, 614, 841, 925
Sau96I	GGNCC	1 cut(s)	506
SchI	GAGTC	1 cut(s)	47
ScrFI	CCNGG	2 cut(s)	347, 504
SduI	GDGCHC	2 cut(s)	77, 499
SfaNI	GCATC	2 cut(s)	513, 538
Sfr274I	CTCGAG	1 cut(s)	714
SlaI	CTCGAG	1 cut(s)	714
SmiMI	CAYNNNNRTG	1 cut(s)	1031
SmlI	CTYRAG	1 cut(s)	714
SmoI	CTYRAG	1 cut(s)	714
Sse9I	AATT	4 cut(s)	173, 208, 537, 767
SsiI	CCGC	2 cut(s)	529, 1049
SspMI	CTAG	4 cut(s)	515, 555, 618, 812
StyD4I	CCNGG	2 cut(s)	345, 502
StyI	CCWWGG	2 cut(s)	354, 447
TaiI	ACGT	1 cut(s)	33
TaqI	TCGA	2 cut(s)	33, 715
TasI	AATT	4 cut(s)	173, 208, 537, 767
TatI	WGTACW	3 cut(s)	20, 139, 789
Tru1I	TTAA	3 cut(s)	645, 666, 912
Tru9I	TTAA	3 cut(s)	645, 666, 912
TseFI	GTSAC	1 cut(s)	26
TseI	GCWGC	6 cut(s)	149, 526, 551, 695, 971, 980
Tsp45I	GTSAC	1 cut(s)	26
TspDTI	ATGAA	3 cut(s)	75, 695, 1014
XagI	CCTNNNNNAGG	1 cut(s)	513
XhoI	CTCGAG	1 cut(s)	714
XspI	CTAG	4 cut(s)	515, 555, 618, 812

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.