Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0004895

Physical Location & Seq

Genomic Coordinates Forward (+)

28828503 .. 28834762

Gene Structure 6260 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00352020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1125 bp

ATGGAGGGCAGAGACTGTCGTAGCCGTGGTTTAGTGGCACATGCCCCTCCCCCTGGTTACTTTGTTAGACTTGAGAATACAAGCGCTGACGACGATTTTTATTTGAGGAAGAAAGTCAGGATGCGCAGGTGGCTTTGTTGTTCTTGTCACGTGGAGGAGTCTTATTCAGCACATGAAAATGAACACCTGAAGAGCCCAAGAAGTTATGATGGGAACAATAAGGGCACTAAGGTGTCCACTCCTTTGAAGTCTGATGTACAAAAGGCAGCACCACCTGTTGAAGTCCCTCCATTGTCTCTGGAGGAGCTGAAAGAGAAGACTGACAATTTTGGATCTAAATCATTGATCGGCGAAGGATCATATGGGAGAGTATACTATGCAAGCATGGATGATGGTAAAGCTGTTGCCGTCAAGAAGCTTGACGTTGCATCCGAGCCCGAGTCAAATGTTGAGTTTCTGACCCAGGTTTCCATGGTTTCTAGACTGAAGCACGACAATCTTGTTGAGCTGCTTGGTTACTGTGTTGAAGGAAGTCTGCGAGTGCTTGCGTATGAGTTTGCCACCATGGGATCTCTGCATGACATATTGCATGGTAGAAAGGGCGTTCAAGGGGCGCAACCTGGTCCAACACTTGACTGGATGCAGCGTGTGAGAATTGCAGTTGATGCAGCCAGGGATTTCAAAGCCAAGATAGCAGATTTTAATCTTTCAAATCAGGCTCCTGATATGGCTGCTCGTCTTCATTCCACTCGAGTTTTGGGAACTTTTGGTTATCATGCTCCAGAGTATGCAATGACTGGTCAATTGACACAGAAGAGTGATGTCTACAGCTTTGGAGTAGTTCTTCTAGAGCTTCTGACTGGGAGGAAACCTGTCGATCATACAATGCCCCGTGGACAGCAGAGTCTTGTGACTTGGGCTACTCCAAGATTGAGTGAAGATAAGGTTAAACAATGTGTAGATCCTAAGCTGAAGGGAGAATATCCTCCGAAAGGAGTCGCAAAGCTAGCAGCTGTGGCAGCTTTGTGTGTACAATATGAAGCTGAGTTCCGGCCAAATATGAGCATTGTTGTTAAGGCGCTACAACCGCTTCTAAAGCCACCTGCACCAGCTCCAGAGTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0030054 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

374

Amino Acids

41.33

Weight (kDa)

8.52

Isoelectric Point (pI)

31.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	113 - 202	2.6e-12	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	114 - 224	8.4e-15	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	226 - 361	8.4e-16	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	228 - 298	3.4e-11	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004895)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43230 AT2G43230 AT3G59350 AT3G59350 AT3G59350 AT3G59350 AT3G59350 AT3G59350
fragaria_vesca	FvH4_4g11280 FvH4_4g11280 FvH4_4g11280
malus_domestica	MD04G1038500.v1.1 MD13G1258500.v1.1
prunus_persica	Prupe.1G189000_v2.0.a1 Prupe.1G189000_v2.0.a1
pyrus_communis	pycom13g25750
rosa_chinensis	RchiOBHm_Chr4g0407431
rosa_laevigata	RLG00000008720
rosa_multiflora	Rmu_sc0004841.1_g000042
rosa_roxburghii	Rroxscaffold_5G00352020
rosa_rugosa	Rorug04G0079700 Rorug04G0079700 Rorug04G0079800 Rorug04G0079900
rosa_samantha	Rh4AG144300 Rh4BG142200 Rh4CG150900 Rh4DG138300
rosa_wichuraiana	Rw0G014480 Rw4G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	117, 1111
AasI	GACNNNNNNGTC	1 cut(s)	903
Acc16I	TGCGCA	1 cut(s)	125
Acc36I	ACCTGC	2 cut(s)	117, 1111
AccI	GTMKAC	2 cut(s)	372, 825
AciI	CCGC	1 cut(s)	1088
AclWI	GGATC	4 cut(s)	340, 364, 577, 956
AcoI	YGGCCR	1 cut(s)	1052
AcuI	CTGAAG	3 cut(s)	209, 506, 992
AcvI	CACGTG	1 cut(s)	151
AfaI	GTAC	2 cut(s)	258, 1032
AfeI	AGCGCT	1 cut(s)	85
AfiI	CCNNNNNNNGG	2 cut(s)	53, 992
AgsI	TTSAA	6 cut(s)	247, 281, 527, 608, 682, 711
AjnI	CCWGG	4 cut(s)	52, 462, 619, 671
AleI	CACNNNNGTG	1 cut(s)	230
Alw26I	GTCTC	2 cut(s)	6, 300
AlwI	GGATC	4 cut(s)	340, 364, 577, 956
AlwNI	CAGNNNCTG	1 cut(s)	15
Ama87I	CYCGRG	2 cut(s)	437, 750
Aor51HI	AGCGCT	1 cut(s)	85
AoxI	GGCC	1 cut(s)	1052
ApeKI	GCWGC	7 cut(s)	266, 508, 643, 668, 731, 1010, 1019
AspLEI	GCGC	4 cut(s)	86, 126, 616, 1081
AspS9I	GGNCC	1 cut(s)	623
AsuNHI	GCTAGC	1 cut(s)	1006
AvaI	CYCGRG	2 cut(s)	437, 750
AvaII	GGWCC	1 cut(s)	623
BaeGI	GKGCMC	1 cut(s)	227
BanII	GRGCYC	2 cut(s)	197, 438
BbrPI	CACGTG	1 cut(s)	151
BbsI	GAAGAC	2 cut(s)	323, 731
BbvI	GCAGC	7 cut(s)	278, 495, 655, 680, 718, 1022, 1031
BccI	CCATC	2 cut(s)	203, 386
BceAI	ACGGC	2 cut(s)	9, 392
BciT130I	CCWGG	4 cut(s)	54, 464, 621, 673
BcoDI	GTCTC	2 cut(s)	6, 300
BfaI	CTAG	3 cut(s)	480, 848, 1007
BfmI	CTRYAG	1 cut(s)	826
BfoI	RGCGCY	2 cut(s)	87, 1082
BfuAI	ACCTGC	2 cut(s)	117, 1111
BisI	GCNGC	7 cut(s)	267, 509, 644, 669, 732, 1011, 1020
BlsI	GCNGC	7 cut(s)	268, 510, 645, 670, 733, 1012, 1021
Bme1390I	CCNGG	4 cut(s)	54, 464, 621, 673
Bme18I	GGWCC	1 cut(s)	623
BmeT110I	CYCGRG	2 cut(s)	437, 750
BmgT120I	GGNCC	1 cut(s)	623
BmiI	GGNNCC	1 cut(s)	720
BmrFI	CCNGG	4 cut(s)	54, 464, 621, 673
BmrI	ACTGGG	1 cut(s)	870
BmsI	GCATC	4 cut(s)	111, 437, 630, 655
BmtI	GCTAGC	1 cut(s)	1010
BmuI	ACTGGG	1 cut(s)	870
BpiI	GAAGAC	2 cut(s)	323, 731
BpmI	CTGGAG	3 cut(s)	320, 765, 1098
Bpu10I	CCTNAGC	1 cut(s)	966
BpuEI	CTTGAG	1 cut(s)	92
BsaAI	YACGTR	1 cut(s)	151
BsaBI	GATNNNNATC	2 cut(s)	337, 702
BsaJI	CCNNGG	7 cut(s)	25, 52, 462, 471, 564, 672, 892
Bsc4I	CCNNNNNNNGG	2 cut(s)	53, 992
Bse1I	ACTGG	3 cut(s)	641, 802, 865
Bse3DI	GCAATG	1 cut(s)	798
Bse8I	GATNNNNATC	2 cut(s)	337, 702
BseBI	CCWGG	4 cut(s)	54, 464, 621, 673
BseDI	CCNNGG	7 cut(s)	25, 52, 462, 471, 564, 672, 892
BseGI	GGATG	4 cut(s)	126, 394, 428, 645
BseJI	GATNNNNATC	2 cut(s)	337, 702
BseLI	CCNNNNNNNGG	2 cut(s)	53, 992
BseMI	GCAATG	1 cut(s)	798
BseMII	CTCAG	1 cut(s)	1035
BseNI	ACTGG	3 cut(s)	641, 802, 865
BseRI	GAGGAG	2 cut(s)	170, 317
BseSI	GKGCMC	1 cut(s)	227
BseXI	GCAGC	7 cut(s)	278, 495, 655, 680, 718, 1022, 1031
BsgI	GTGCAG	1 cut(s)	1089
BshFI	GGCC	1 cut(s)	1054
BsiHKCI	CYCGRG	2 cut(s)	437, 750
BsiSI	CCGG	1 cut(s)	1051
BslFI	GGGAC	1 cut(s)	269
BslI	CCNNNNNNNGG	2 cut(s)	53, 992
BsmAI	GTCTC	2 cut(s)	6, 300
BsmFI	GGGAC	1 cut(s)	269
BsnI	GGCC	1 cut(s)	1054
BsoBI	CYCGRG	2 cut(s)	437, 750
Bsp1286I	GDGCHC	3 cut(s)	197, 227, 438
Bsp1407I	TGTACA	2 cut(s)	256, 1030
Bsp143I	GATC	6 cut(s)	332, 345, 356, 569, 877, 961
Bsp19I	CCATGG	2 cut(s)	471, 564
BspACI	CCGC	1 cut(s)	1088
BspANI	GGCC	1 cut(s)	1054
BspCNI	CTCAG	1 cut(s)	1036
BspLI	GGNNCC	1 cut(s)	720
BspMI	ACCTGC	2 cut(s)	117, 1111
BspOI	GCTAGC	1 cut(s)	1010
BspPI	GGATC	4 cut(s)	340, 364, 577, 956
BspQI	GCTCTTC	1 cut(s)	185
BsrDI	GCAATG	1 cut(s)	798
BsrGI	TGTACA	2 cut(s)	256, 1030
BsrI	ACTGG	3 cut(s)	641, 802, 865
BssECI	CCNNGG	7 cut(s)	25, 52, 462, 471, 564, 672, 892
BssMI	GATC	6 cut(s)	332, 345, 356, 569, 877, 961
BssNAI	GTATAC	1 cut(s)	373
BssT1I	CCWWGG	2 cut(s)	471, 564
Bst1107I	GTATAC	1 cut(s)	373
Bst2UI	CCWGG	4 cut(s)	54, 464, 621, 673
Bst4CI	ACNGT	2 cut(s)	17, 521
Bst6I	CTCTTC	2 cut(s)	185, 809
BstAPI	GCANNNNNTGC	1 cut(s)	665
BstAUI	TGTACA	2 cut(s)	256, 1030
BstBAI	YACGTR	1 cut(s)	151
BstC8I	GCNNGC	3 cut(s)	382, 546, 1008
BstDEI	CTNAG	3 cut(s)	228, 966, 1044
BstDSI	CCRYGG	4 cut(s)	25, 471, 564, 892
BstENI	CCTNNNNNAGG	1 cut(s)	990
BstF5I	GGATG	4 cut(s)	126, 394, 428, 645
BstH2I	RGCGCY	2 cut(s)	87, 1082
BstHHI	GCGC	4 cut(s)	86, 126, 616, 1081
BstKTI	GATC	6 cut(s)	335, 348, 359, 572, 880, 964
BstMAI	GTCTC	2 cut(s)	6, 300
BstMBI	GATC	6 cut(s)	332, 345, 356, 569, 877, 961
BstMWI	GCNNNNNNNGC	8 cut(s)	130, 665, 692, 1007, 1016, 1019, 1087, 1096
BstNI	CCWGG	4 cut(s)	54, 464, 621, 673
BstNSI	RCATGY	1 cut(s)	44
BstSCI	CCNGG	4 cut(s)	52, 462, 619, 671
BstSFI	CTRYAG	1 cut(s)	826
BstSLI	GKGCMC	1 cut(s)	227
BstV1I	GCAGC	7 cut(s)	278, 495, 655, 680, 718, 1022, 1031
BstV2I	GAAGAC	2 cut(s)	323, 731
BstX2I	RGATCY	3 cut(s)	332, 569, 961
BstYI	RGATCY	3 cut(s)	332, 569, 961
BstZ17I	GTATAC	1 cut(s)	373
BsuRI	GGCC	1 cut(s)	1054
BtgI	CCRYGG	4 cut(s)	25, 471, 564, 892
BtsCI	GGATG	4 cut(s)	126, 394, 428, 645
BveI	ACCTGC	2 cut(s)	117, 1111
Cac8I	GCNNGC	3 cut(s)	382, 546, 1008
CaiI	CAGNNNCTG	1 cut(s)	15
CfoI	GCGC	4 cut(s)	86, 126, 616, 1081
Cfr13I	GGNCC	1 cut(s)	623
CsiI	ACCWGGT	1 cut(s)	619
Csp6I	GTAC	2 cut(s)	257, 1031
CviAII	CATG	8 cut(s)	41, 173, 385, 472, 565, 578, 590, 776
CviQI	GTAC	2 cut(s)	257, 1031
DdeI	CTNAG	3 cut(s)	228, 966, 1044
DpnI	GATC	6 cut(s)	334, 347, 358, 571, 879, 963
DpnII	GATC	6 cut(s)	332, 345, 356, 569, 877, 961
DrdI	GACNNNNNNGTC	1 cut(s)	903
DseDI	GACNNNNNNGTC	1 cut(s)	903
EaeI	YGGCCR	1 cut(s)	1052
Eam1104I	CTCTTC	2 cut(s)	185, 809
EarI	CTCTTC	2 cut(s)	185, 809
Eco130I	CCWWGG	2 cut(s)	471, 564
Eco24I	GRGCYC	2 cut(s)	197, 438
Eco47I	GGWCC	1 cut(s)	623
Eco47III	AGCGCT	1 cut(s)	85
Eco57I	CTGAAG	3 cut(s)	209, 506, 992
Eco72I	CACGTG	1 cut(s)	151
Eco88I	CYCGRG	2 cut(s)	437, 750
EcoNI	CCTNNNNNAGG	1 cut(s)	990
EcoRII	CCWGG	4 cut(s)	52, 462, 619, 671
EcoT14I	CCWWGG	2 cut(s)	471, 564
EcoT38I	GRGCYC	2 cut(s)	197, 438
ErhI	CCWWGG	2 cut(s)	471, 564
FaeI	CATG	8 cut(s)	44, 176, 388, 475, 568, 581, 593, 779
FaqI	GGGAC	1 cut(s)	269
FatI	CATG	8 cut(s)	40, 172, 384, 471, 564, 577, 589, 775
FauNDI	CATATG	1 cut(s)	361
FblI	GTMKAC	2 cut(s)	372, 825
Fnu4HI	GCNGC	7 cut(s)	267, 509, 644, 669, 732, 1011, 1020
FokI	GGATG	4 cut(s)	133, 401, 415, 652
FriOI	GRGCYC	2 cut(s)	197, 438
Fsp4HI	GCNGC	7 cut(s)	267, 509, 644, 669, 732, 1011, 1020
FspBI	CTAG	3 cut(s)	480, 848, 1007
FspI	TGCGCA	1 cut(s)	125
GlaI	GCGC	4 cut(s)	85, 125, 615, 1080
GluI	GCNGC	7 cut(s)	267, 509, 644, 669, 732, 1011, 1020
GsuI	CTGGAG	3 cut(s)	320, 765, 1098
HaeII	RGCGCY	2 cut(s)	87, 1082
HaeIII	GGCC	1 cut(s)	1054
HapII	CCGG	1 cut(s)	1051
HhaI	GCGC	4 cut(s)	86, 126, 616, 1081
Hin1II	CATG	8 cut(s)	44, 176, 388, 475, 568, 581, 593, 779
Hin6I	GCGC	4 cut(s)	84, 124, 614, 1079
HinP1I	GCGC	4 cut(s)	84, 124, 614, 1079
HindIII	AAGCTT	1 cut(s)	416
HinfI	GANTC	5 cut(s)	158, 440, 904, 996, 1118
HpaII	CCGG	1 cut(s)	1051
Hpy166II	GTNNAC	5 cut(s)	237, 373, 826, 896, 1031
Hpy188I	TCNGA	5 cut(s)	253, 433, 459, 858, 990
Hpy188III	TCNNGA	9 cut(s)	118, 299, 412, 480, 722, 782, 848, 1115, 1122
Hpy8I	GTNNAC	5 cut(s)	237, 373, 826, 896, 1031
Hpy99I	CGWCG	1 cut(s)	95
HpyAV	CCTTC	3 cut(s)	347, 521, 967
HpyCH4III	ACNGT	2 cut(s)	17, 521
HpyCH4IV	ACGT	2 cut(s)	150, 423
HpyCH4V	TGCA	9 cut(s)	380, 428, 577, 589, 643, 659, 668, 791, 1106
HpyF10VI	GCNNNNNNNGC	8 cut(s)	130, 665, 692, 1007, 1016, 1019, 1087, 1096
HpyF3I	CTNAG	3 cut(s)	228, 966, 1044
HpySE526I	ACGT	2 cut(s)	150, 423
Hsp92II	CATG	8 cut(s)	44, 176, 388, 475, 568, 581, 593, 779
HspAI	GCGC	4 cut(s)	84, 124, 614, 1079
Kzo9I	GATC	6 cut(s)	332, 345, 356, 569, 877, 961
LguI	GCTCTTC	1 cut(s)	185
LmnI	GCTCC	4 cut(s)	304, 724, 784, 1117
Lsp1109I	GCAGC	7 cut(s)	278, 495, 655, 680, 718, 1022, 1031
LweI	GCATC	4 cut(s)	111, 437, 630, 655
MabI	ACCWGGT	1 cut(s)	619
MaeI	CTAG	3 cut(s)	480, 848, 1007
MaeII	ACGT	2 cut(s)	150, 423
MaeIII	GTNAC	4 cut(s)	56, 146, 515, 910
MalI	GATC	6 cut(s)	334, 347, 358, 571, 879, 963
MboI	GATC	6 cut(s)	332, 345, 356, 569, 877, 961
MboII	GAAGA	7 cut(s)	121, 202, 328, 731, 826, 836, 950
MfeI	CAATTG	1 cut(s)	803
MflI	RGATCY	3 cut(s)	332, 569, 961
MhlI	GDGCHC	3 cut(s)	197, 227, 438
MluCI	AATT	3 cut(s)	325, 654, 803
MlyI	GAGTC	4 cut(s)	167, 449, 913, 1005
MmeI	TCCRAC	1 cut(s)	650
MnlI	CCTC	7 cut(s)	57, 99, 148, 295, 297, 858, 996
MseI	TTAA	3 cut(s)	702, 948, 1074
MslI	CAYNNNNRTG	2 cut(s)	177, 230
MspA1I	CMGCKG	1 cut(s)	1013
MspI	CCGG	1 cut(s)	1051
MspR9I	CCNGG	4 cut(s)	54, 464, 621, 673
MunI	CAATTG	1 cut(s)	803
MvaI	CCWGG	4 cut(s)	54, 464, 621, 673
MwoI	GCNNNNNNNGC	8 cut(s)	130, 665, 692, 1007, 1016, 1019, 1087, 1096
NcoI	CCATGG	2 cut(s)	471, 564
NdeI	CATATG	1 cut(s)	361
NdeII	GATC	6 cut(s)	332, 345, 356, 569, 877, 961
NheI	GCTAGC	1 cut(s)	1006
NlaIII	CATG	8 cut(s)	44, 176, 388, 475, 568, 581, 593, 779
NlaIV	GGNNCC	1 cut(s)	720
NmuCI	GTSAC	2 cut(s)	146, 910
NsbI	TGCGCA	1 cut(s)	125
NspI	RCATGY	1 cut(s)	44
OliI	CACNNNNGTG	1 cut(s)	230
PaeR7I	CTCGAG	1 cut(s)	750
PaqCI	CACCTGC	2 cut(s)	117, 1111
PciSI	GCTCTTC	1 cut(s)	185
PcsI	WCGNNNNNNNCGW	1 cut(s)	429
PkrI	GCNGC	7 cut(s)	268, 510, 645, 670, 733, 1012, 1021
PleI	GAGTC	4 cut(s)	166, 448, 912, 1004
PmaCI	CACGTG	1 cut(s)	151
PmlI	CACGTG	1 cut(s)	151
PpsI	GAGTC	4 cut(s)	166, 448, 912, 1004
Ppu21I	YACGTR	1 cut(s)	151
Psp6I	CCWGG	4 cut(s)	52, 462, 619, 671
PspCI	CACGTG	1 cut(s)	151
PspGI	CCWGG	4 cut(s)	52, 462, 619, 671
PspN4I	GGNNCC	1 cut(s)	720
PspPI	GGNCC	1 cut(s)	623
PspXI	VCTCGAGB	1 cut(s)	750
PstNI	CAGNNNCTG	1 cut(s)	15
PsuI	RGATCY	3 cut(s)	332, 569, 961
PvuII	CAGCTG	1 cut(s)	1013
RsaI	GTAC	2 cut(s)	258, 1032
RsaNI	GTAC	2 cut(s)	257, 1031
RseI	CAYNNNNRTG	2 cut(s)	177, 230
SapI	GCTCTTC	1 cut(s)	185
SaqAI	TTAA	3 cut(s)	702, 948, 1074
SatI	GCNGC	7 cut(s)	267, 509, 644, 669, 732, 1011, 1020
Sau3AI	GATC	6 cut(s)	332, 345, 356, 569, 877, 961
Sau96I	GGNCC	1 cut(s)	623
SchI	GAGTC	4 cut(s)	167, 449, 913, 1005
ScrFI	CCNGG	4 cut(s)	54, 464, 621, 673
SduI	GDGCHC	3 cut(s)	197, 227, 438
SexAI	ACCWGGT	1 cut(s)	619
SfaNI	GCATC	4 cut(s)	111, 437, 630, 655
SfcI	CTRYAG	1 cut(s)	826
Sfr274I	CTCGAG	1 cut(s)	750
SinI	GGWCC	1 cut(s)	623
SlaI	CTCGAG	1 cut(s)	750
SmiMI	CAYNNNNRTG	2 cut(s)	177, 230
SmlI	CTYRAG	2 cut(s)	71, 750
SmoI	CTYRAG	2 cut(s)	71, 750
Sse9I	AATT	3 cut(s)	325, 654, 803
SsiI	CCGC	1 cut(s)	1088
SspMI	CTAG	3 cut(s)	480, 848, 1007
StyD4I	CCNGG	4 cut(s)	52, 462, 619, 671
StyI	CCWWGG	2 cut(s)	471, 564
TaaI	ACNGT	2 cut(s)	17, 521
TaiI	ACGT	2 cut(s)	153, 426
TaqI	TCGA	2 cut(s)	751, 876
TasI	AATT	3 cut(s)	325, 654, 803
TatI	WGTACW	2 cut(s)	256, 1030
Tru1I	TTAA	3 cut(s)	702, 948, 1074
Tru9I	TTAA	3 cut(s)	702, 948, 1074
TseFI	GTSAC	2 cut(s)	146, 910
TseI	GCWGC	7 cut(s)	266, 508, 643, 668, 731, 1010, 1019
Tsp45I	GTSAC	2 cut(s)	146, 910
TspDTI	ATGAA	4 cut(s)	189, 195, 731, 1053
VpaK11BI	GGWCC	1 cut(s)	623
XagI	CCTNNNNNAGG	1 cut(s)	990
XbaI	TCTAGA	2 cut(s)	479, 847
XceI	RCATGY	1 cut(s)	44
XcmI	CCANNNNNNNNNTGG	2 cut(s)	633, 754
XhoI	CTCGAG	1 cut(s)	750
XmiI	GTMKAC	2 cut(s)	372, 825
XspI	CTAG	3 cut(s)	480, 848, 1007

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.