MD14G1001400.v1.1

Thioredoxin-like 1-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006818
Physical Location & Seq
Forward (+)
276533 .. 278702
2170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1001400.v1.1.491

Sequence Viewer

Length: 867 bp
ATGGCAGAGGTTTTGAGCAAAACCAGTCTGTTGTCGTCCTGGCATCATCATCATCAGTATCATCAAAACAATCAAAGCAGCATCTCCGTTTTCCCAAAGAGTTGTAATTACAATAAGTCAAGGTCTTTCTCTTGCTCTCTCAAACTGAGTCCGGAGATTTCATCACCATCGTTATCGACAACGAGTGATTTTCACGGCAAAAATATTGCTTTTCAAGCCACCAGCATCACGAGTTCCCAACCCCACGCTTCTTCTGCCGTTAATTCCCAGCAGTCTGGACGTCGGATTGGGAAAGCTCTGAAATGGTGGGAGAAGGGGAATCAGCCCAACATGAAAGAGGTGGTATCTGCGGAGGACCTAGTGGAATCCCTTTCAAATGCCGGGGATAAGCTTGTGATCGTTGATTTCTTCTCTCCTGGCTGCGGTGGCTGCAAGGCTCTTCATCCCAAGATATGTCAGCTGGCAGAGATGACCCCAGATGTTCAATTCCTTCAGGTGAACTACGAGGAACACAAGTCCATGTGTTACAGTCTCAATGTTCATGTCCTGCCCTTCTTCCGCTTTTACAGAGGTGCTCATGGTCGCCTTTGCAGCTTCAGCTGTACCAACGCCACGATCAAGAAGTTTAGAGATGCATTAGCCAAGCACAAACCAGAGCGGTGCAGCCTTGGGCCAACAAAAGGGCTGGAGGAGAAAGAGCTCCTAGCTCTTGCTACCAACAAAGACCTCTCATTTACCTACACTCCTAAACCAACAGAAGATGTGCCTGCCGAGGAGGAAGTGATTGTGGCTGAAGAGACACCATTTCGTCTTCCTCTTCCATTGGCAACCTCAAAGTCTGCCCAAGTGGTCACTGCCTGGAGATAA

Protein Analysis

289

Amino Acids

31.99

Weight (kDa)

8.66

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 118 - 192 9.4e-14 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 283
AccBSI CCGCTC 1 cut(s) 658
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 4 cut(s) 350, 423, 559, 658
AcuI CTGAAG 3 cut(s) 476, 580, 813
AcyI GRCGYC 1 cut(s) 280
AfaI GTAC 1 cut(s) 604
AfiI CCNNNNNNNGG 2 cut(s) 422, 680
AgsI TTSAA 3 cut(s) 215, 375, 485
AjnI CCWGG 3 cut(s) 38, 415, 857
AluBI AGCT 7 cut(s) 296, 391, 460, 594, 600, 700, 707
AluI AGCT 7 cut(s) 296, 391, 460, 594, 600, 700, 707
Alw21I GWGCWC 2 cut(s) 577, 702
Alw26I GTCTC 2 cut(s) 536, 791
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 1 cut(s) 671
ApeKI GCWGC 5 cut(s) 78, 420, 429, 591, 663
AspS9I GGNCC 2 cut(s) 355, 671
AsuC2I CCSGG 1 cut(s) 382
AsuHPI GGTGA 2 cut(s) 156, 508
AvaII GGWCC 1 cut(s) 355
BanII GRGCYC 1 cut(s) 702
BauI CACGAG 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 803
Bbv12I GWGCWC 2 cut(s) 577, 702
BbvI GCAGC 5 cut(s) 90, 407, 416, 603, 675
BccI CCATC 1 cut(s) 175
BceAI ACGGC 2 cut(s) 211, 242
BciT130I CCWGG 3 cut(s) 40, 417, 859
BcnI CCSGG 1 cut(s) 382
BcoDI GTCTC 2 cut(s) 536, 791
BfaI CTAG 2 cut(s) 359, 704
BisI GCNGC 5 cut(s) 79, 421, 430, 592, 664
BlsI GCNGC 5 cut(s) 80, 422, 431, 593, 665
Bme1390I CCNGG 4 cut(s) 40, 382, 417, 859
Bme18I GGWCC 1 cut(s) 355
BmgT120I GGNCC 2 cut(s) 355, 671
BmrFI CCNGG 4 cut(s) 40, 382, 417, 859
BmsI GCATC 4 cut(s) 52, 90, 234, 622
BpiI GAAGAC 1 cut(s) 803
BpmI CTGGAG 1 cut(s) 707
BpuMI CCSGG 1 cut(s) 382
BsaHI GRCGYC 1 cut(s) 280
BsaJI CCNNGG 3 cut(s) 381, 667, 771
BsaWI WCCGGW 1 cut(s) 151
BsaXI ACNNNNNCTCC 2 cut(s) 727, 757
Bsc4I CCNNNNNNNGG 2 cut(s) 422, 680
Bse1I ACTGG 1 cut(s) 24
BseAI TCCGGA 1 cut(s) 151
BseBI CCWGG 3 cut(s) 40, 417, 859
BseDI CCNNGG 3 cut(s) 381, 667, 771
BseGI GGATG 1 cut(s) 442
BseLI CCNNNNNNNGG 2 cut(s) 422, 680
BseMII CTCAG 1 cut(s) 137
BseNI ACTGG 1 cut(s) 24
BseRI GAGGAG 2 cut(s) 704, 788
BseXI GCAGC 5 cut(s) 90, 407, 416, 603, 675
BseYI CCCAGC 1 cut(s) 267
BsgI GTGCAG 1 cut(s) 682
BshFI GGCC 1 cut(s) 673
BsiHKAI GWGCWC 2 cut(s) 577, 702
BsiSI CCGG 2 cut(s) 152, 381
BslI CCNNNNNNNGG 2 cut(s) 422, 680
BsmAI GTCTC 2 cut(s) 536, 791
BsnI GGCC 1 cut(s) 673
Bsp1286I GDGCHC 2 cut(s) 577, 702
Bsp13I TCCGGA 1 cut(s) 151
Bsp143I GATC 2 cut(s) 396, 615
BspACI CCGC 4 cut(s) 350, 423, 559, 658
BspANI GGCC 1 cut(s) 673
BspCNI CTCAG 1 cut(s) 138
BspEI TCCGGA 1 cut(s) 151
BspQI GCTCTTC 1 cut(s) 444
BsrBI CCGCTC 1 cut(s) 658
BsrI ACTGG 1 cut(s) 24
BssECI CCNNGG 3 cut(s) 381, 667, 771
BssMI GATC 2 cut(s) 396, 615
BssNI GRCGYC 1 cut(s) 280
BssSI CACGAG 1 cut(s) 229
BssT1I CCWWGG 1 cut(s) 667
Bst2BI CACGAG 1 cut(s) 229
Bst2UI CCWGG 3 cut(s) 40, 417, 859
Bst4CI ACNGT 1 cut(s) 530
Bst6I CTCTTC 3 cut(s) 444, 789, 822
BstACI GRCGYC 1 cut(s) 280
BstC8I GCNNGC 2 cut(s) 462, 768
BstDEI CTNAG 1 cut(s) 146
BstF5I GGATG 1 cut(s) 442
BstKTI GATC 2 cut(s) 399, 618
BstMAI GTCTC 2 cut(s) 536, 791
BstMBI GATC 2 cut(s) 396, 615
BstMWI GCNNNNNNNGC 6 cut(s) 215, 254, 426, 429, 591, 597
BstNI CCWGG 3 cut(s) 40, 417, 859
BstSCI CCNGG 4 cut(s) 38, 380, 415, 857
BstV1I GCAGC 5 cut(s) 90, 407, 416, 603, 675
BstV2I GAAGAC 1 cut(s) 803
BstXI CCANNNNNNTGG 1 cut(s) 275
BsuRI GGCC 1 cut(s) 673
BtsCI GGATG 1 cut(s) 442
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 1 cut(s) 852
Cac8I GCNNGC 2 cut(s) 462, 768
Cfr13I GGNCC 2 cut(s) 355, 671
Csp6I GTAC 1 cut(s) 603
CviAII CATG 4 cut(s) 331, 520, 542, 578
CviQI GTAC 1 cut(s) 603
DdeI CTNAG 1 cut(s) 146
DpnI GATC 2 cut(s) 398, 617
DpnII GATC 2 cut(s) 396, 615
Eam1104I CTCTTC 3 cut(s) 444, 789, 822
EarI CTCTTC 3 cut(s) 444, 789, 822
Ecl136II GAGCTC 1 cut(s) 700
Eco130I CCWWGG 1 cut(s) 667
Eco24I GRGCYC 1 cut(s) 702
Eco47I GGWCC 1 cut(s) 355
Eco53kI GAGCTC 1 cut(s) 700
Eco57I CTGAAG 3 cut(s) 476, 580, 813
EcoICRI GAGCTC 1 cut(s) 700
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 3 cut(s) 38, 415, 857
EcoT14I CCWWGG 1 cut(s) 667
EcoT22I ATGCAT 1 cut(s) 637
EcoT38I GRGCYC 1 cut(s) 702
ErhI CCWWGG 1 cut(s) 667
FaeI CATG 4 cut(s) 334, 523, 545, 581
FaiI YATR 5 cut(s) 332, 454, 521, 543, 579
FatI CATG 4 cut(s) 330, 519, 541, 577
Fnu4HI GCNGC 5 cut(s) 79, 421, 430, 592, 664
FokI GGATG 1 cut(s) 429
FriOI GRGCYC 1 cut(s) 702
Fsp4HI GCNGC 5 cut(s) 79, 421, 430, 592, 664
FspBI CTAG 2 cut(s) 359, 704
GluI GCNGC 5 cut(s) 79, 421, 430, 592, 664
GsaI CCCAGC 1 cut(s) 271
GsuI CTGGAG 1 cut(s) 707
HaeIII GGCC 1 cut(s) 673
HapII CCGG 2 cut(s) 152, 381
Hin1I GRCGYC 1 cut(s) 280
Hin1II CATG 4 cut(s) 334, 523, 545, 581
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 3 cut(s) 148, 319, 365
HpaII CCGG 2 cut(s) 152, 381
HphI GGTGA 2 cut(s) 156, 508
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 2 cut(s) 285, 300
Hpy188III TCNNGA 4 cut(s) 152, 229, 276, 619
Hpy8I GTNNAC 1 cut(s) 499
Hpy99I CGWCG 1 cut(s) 285
HpyAV CCTTC 3 cut(s) 307, 500, 562
HpyCH4III ACNGT 1 cut(s) 530
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 4 cut(s) 432, 591, 635, 663
HpyF10VI GCNNNNNNNGC 6 cut(s) 215, 254, 426, 429, 591, 597
HpyF3I CTNAG 1 cut(s) 146
HpySE526I ACGT 1 cut(s) 280
Hsp92I GRCGYC 1 cut(s) 280
Hsp92II CATG 4 cut(s) 334, 523, 545, 581
Kpn2I TCCGGA 1 cut(s) 151
Kzo9I GATC 2 cut(s) 396, 615
LguI GCTCTTC 1 cut(s) 444
LmnI GCTCC 1 cut(s) 705
Lsp1109I GCAGC 5 cut(s) 90, 407, 416, 603, 675
LweI GCATC 4 cut(s) 52, 90, 234, 622
MaeI CTAG 2 cut(s) 359, 704
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 2 cut(s) 524, 850
MalI GATC 2 cut(s) 398, 617
MbiI CCGCTC 1 cut(s) 658
MboI GATC 2 cut(s) 396, 615
MboII GAAGA 8 cut(s) 243, 400, 431, 547, 770, 803, 806, 809
MhlI GDGCHC 2 cut(s) 577, 702
MluCI AATT 3 cut(s) 106, 262, 485
MlyI GAGTC 1 cut(s) 157
MmeI TCCRAC 1 cut(s) 263
Mph1103I ATGCAT 1 cut(s) 637
MroI TCCGGA 1 cut(s) 151
MseI TTAA 1 cut(s) 261
MspA1I CMGCKG 2 cut(s) 460, 600
MspI CCGG 2 cut(s) 152, 381
MspR9I CCNGG 4 cut(s) 40, 382, 417, 859
MvaI CCWGG 3 cut(s) 40, 417, 859
MwoI GCNNNNNNNGC 6 cut(s) 215, 254, 426, 429, 591, 597
NciI CCSGG 1 cut(s) 382
NdeII GATC 2 cut(s) 396, 615
NlaIII CATG 4 cut(s) 334, 523, 545, 581
NmeAIII GCCGAG 1 cut(s) 796
NmuCI GTSAC 1 cut(s) 850
NsiI ATGCAT 1 cut(s) 637
PciSI GCTCTTC 1 cut(s) 444
PfeI GAWTC 2 cut(s) 319, 365
PkrI GCNGC 5 cut(s) 80, 422, 431, 593, 665
PleI GAGTC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 156
PpuMI RGGWCCY 1 cut(s) 355
Psp124BI GAGCTC 1 cut(s) 702
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 3 cut(s) 38, 415, 857
PspFI CCCAGC 1 cut(s) 267
PspGI CCWGG 3 cut(s) 38, 415, 857
PspPI GGNCC 2 cut(s) 355, 671
PspPPI RGGWCCY 1 cut(s) 355
PvuII CAGCTG 2 cut(s) 460, 600
RsaI GTAC 1 cut(s) 604
RsaNI GTAC 1 cut(s) 603
SacI GAGCTC 1 cut(s) 702
SapI GCTCTTC 1 cut(s) 444
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 5 cut(s) 79, 421, 430, 592, 664
Sau3AI GATC 2 cut(s) 396, 615
Sau96I GGNCC 2 cut(s) 355, 671
SchI GAGTC 1 cut(s) 157
ScrFI CCNGG 4 cut(s) 40, 382, 417, 859
SduI GDGCHC 2 cut(s) 577, 702
SfaNI GCATC 4 cut(s) 52, 90, 234, 622
SinI GGWCC 1 cut(s) 355
Sse9I AATT 3 cut(s) 106, 262, 485
SsiI CCGC 4 cut(s) 350, 423, 559, 658
SspI AATATT 1 cut(s) 205
SspMI CTAG 2 cut(s) 359, 704
SstI GAGCTC 1 cut(s) 702
StyD4I CCNGG 4 cut(s) 38, 380, 415, 857
StyI CCWWGG 1 cut(s) 667
TaaI ACNGT 1 cut(s) 530
TaiI ACGT 1 cut(s) 283
TaqI TCGA 1 cut(s) 176
TasI AATT 3 cut(s) 106, 262, 485
TfiI GAWTC 2 cut(s) 319, 365
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 1 cut(s) 859
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 5 cut(s) 78, 420, 429, 591, 663
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 4 cut(s) 150, 347, 431, 530
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 1 cut(s) 859
VpaK11BI GGWCC 1 cut(s) 355
XspI CTAG 2 cut(s) 359, 704
ZraI GACGTC 1 cut(s) 281
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.