Rh3BG373200

Thioredoxin-like 1-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0006818
Physical Location & Seq
Forward (+)
47127365 .. 47128886
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG373200.1

Sequence Viewer

Length: 849 bp
ATGGCGGATGTGGTTGTGAGCAAAACCAACTTGCTTTCATCATGCCACCACCATGATCAACACATCTCTGTGTTTCCAAGGACTTGGAATCAATCATCGCTGTCATTCTCATCAAAGACGGACTTTCATGGCAAACGGATTGGGCTTCAACAACTTAGACGACCCTTTCCCACTGCAAGGGATCCGCCGCAATTCCAAGCCATTAATTCACAGCTTCAGGCGCCGGGTTTTCGAATCGGTAAAGCTCATAGATGGTGGGAAAAGGGGATCCAACCCAACATGAAAGAGGTGACATCAGCACAAGACCTTGTGGATTCCCTCTCAAACGCCGGAGATAAGCTTGTCGTCCTTGATTTCTTCTCTCCCGGTTGTGGTGGCTGCAAAGCTCTTCATCCTAAGATATGTCAGTTTGCTGAGATGAACCCAGATGTTCAGTTTCTTCAGGTGAACTATGAGGAACACAAATCCATGTGTTATAGCCTCAATGTTCATGTCCTACCCTTCTTTCGCTTCTACAGAGGAGCTCATGGTCGTGTATGCAGCTTTAGCTGCACAAACGCCACGATCAAAAAATTTAAAGATGCGTTAGCCAAGCACAAGCCAGAGCGCTGCAGCCTTGGGCCAACTAAGGGGTTGGAGGAGAAAGAGCTCCTCGCACTTTCTGCTAATAAAGATCTTAAATTCACCTACACCCCTAAACAAGTTGAATTTGTGCCTGCAAAAGCAGCAGATGAAATGATTGTGGCAGCACCATCTCGTTCAAACTTGGAATCTCTTCCTCTGCCTTCAACAACATTGAAGTCTGGTCAAATCTCCGAGGAGAGAAACATGGTCAGTGCCGGGAGATGA

Protein Analysis

282

Amino Acids

31.53

Weight (kDa)

9.25

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 107 - 176 1.3e-12 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 220
AciI CCGC 3 cut(s) 5, 185, 188
AclWI GGATC 4 cut(s) 176, 189, 262, 275
AcsI RAATTY 3 cut(s) 572, 680, 707
AcuI CTGAAG 2 cut(s) 200, 425
AcyI GRCGYC 1 cut(s) 221
AfeI AGCGCT 1 cut(s) 608
AfiI CCNNNNNNNGG 3 cut(s) 177, 371, 629
AgsI TTSAA 5 cut(s) 149, 707, 762, 789, 799
AluBI AGCT 8 cut(s) 214, 245, 340, 386, 524, 543, 549, 649
AluI AGCT 8 cut(s) 214, 245, 340, 386, 524, 543, 549, 649
Alw21I GWGCWC 2 cut(s) 526, 651
AlwI GGATC 4 cut(s) 176, 189, 262, 275
Aor51HI AGCGCT 1 cut(s) 608
AoxI GGCC 1 cut(s) 620
ApeKI GCWGC 7 cut(s) 378, 540, 549, 609, 612, 725, 746
ApoI RAATTY 3 cut(s) 572, 680, 707
AseI ATTAAT 1 cut(s) 204
Asp700I GAANNNNTTC 1 cut(s) 774
AspLEI GCGC 2 cut(s) 223, 609
AspS9I GGNCC 1 cut(s) 620
AsuC2I CCSGG 3 cut(s) 225, 366, 841
AsuHPI GGTGA 3 cut(s) 301, 457, 676
AsuII TTCGAA 1 cut(s) 232
BamHI GGATCC 2 cut(s) 181, 267
BanI GGYRCC 1 cut(s) 220
BanII GRGCYC 2 cut(s) 526, 651
Bbv12I GWGCWC 2 cut(s) 526, 651
BbvI GCAGC 7 cut(s) 365, 536, 552, 596, 624, 737, 758
BccI CCATC 2 cut(s) 246, 760
BclI TGATCA 1 cut(s) 55
BcnI CCSGG 3 cut(s) 225, 366, 841
BfmI CTRYAG 2 cut(s) 514, 610
BfoI RGCGCY 2 cut(s) 224, 610
BglII AGATCT 1 cut(s) 673
BisI GCNGC 8 cut(s) 188, 379, 541, 550, 610, 613, 726, 747
BlsI GCNGC 8 cut(s) 189, 380, 542, 551, 611, 614, 727, 748
Bme1390I CCNGG 3 cut(s) 225, 366, 841
BmgT120I GGNCC 1 cut(s) 620
BmiI GGNNCC 3 cut(s) 183, 222, 269
BmrFI CCNGG 3 cut(s) 225, 366, 841
BmsI GCATC 1 cut(s) 571
Bpu14I TTCGAA 1 cut(s) 232
BpuMI CCSGG 3 cut(s) 225, 366, 841
BsaHI GRCGYC 1 cut(s) 221
BsaJI CCNNGG 3 cut(s) 77, 616, 816
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 371, 629
BseDI CCNNGG 3 cut(s) 77, 616, 816
BseGI GGATG 2 cut(s) 13, 391
BseLI CCNNNNNNNGG 3 cut(s) 177, 371, 629
BseMII CTCAG 1 cut(s) 405
BseRI GAGGAG 4 cut(s) 534, 641, 653, 833
BseXI GCAGC 7 cut(s) 365, 536, 552, 596, 624, 737, 758
BsgI GTGCAG 1 cut(s) 535
BshFI GGCC 1 cut(s) 622
BshNI GGYRCC 1 cut(s) 220
BsiHKAI GWGCWC 2 cut(s) 526, 651
BsiSI CCGG 4 cut(s) 224, 330, 366, 840
BslI CCNNNNNNNGG 3 cut(s) 177, 371, 629
BsnI GGCC 1 cut(s) 622
Bsp119I TTCGAA 1 cut(s) 232
Bsp1286I GDGCHC 2 cut(s) 526, 651
Bsp143I GATC 5 cut(s) 55, 181, 267, 564, 673
BspACI CCGC 3 cut(s) 5, 185, 188
BspANI GGCC 1 cut(s) 622
BspCNI CTCAG 1 cut(s) 406
BspLI GGNNCC 3 cut(s) 183, 222, 269
BspMAI CTGCAG 1 cut(s) 614
BspPI GGATC 4 cut(s) 176, 189, 262, 275
BspQI GCTCTTC 1 cut(s) 393
BspT104I TTCGAA 1 cut(s) 232
BspT107I GGYRCC 1 cut(s) 220
BssECI CCNNGG 3 cut(s) 77, 616, 816
BssMI GATC 5 cut(s) 55, 181, 267, 564, 673
BssNI GRCGYC 1 cut(s) 221
BssT1I CCWWGG 2 cut(s) 77, 616
Bst6I CTCTTC 2 cut(s) 393, 780
BstACI GRCGYC 1 cut(s) 221
BstAPI GCANNNNNTGC 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 232
BstC8I GCNNGC 1 cut(s) 717
BstDEI CTNAG 4 cut(s) 155, 396, 414, 627
BstF5I GGATG 2 cut(s) 13, 391
BstH2I RGCGCY 2 cut(s) 224, 610
BstHHI GCGC 2 cut(s) 223, 609
BstKTI GATC 5 cut(s) 58, 184, 270, 567, 676
BstMBI GATC 5 cut(s) 55, 181, 267, 564, 673
BstMWI GCNNNNNNNGC 5 cut(s) 220, 546, 549, 662, 725
BstSCI CCNGG 3 cut(s) 223, 364, 839
BstSFI CTRYAG 2 cut(s) 514, 610
BstV1I GCAGC 7 cut(s) 365, 536, 552, 596, 624, 737, 758
BstX2I RGATCY 3 cut(s) 181, 267, 673
BstXI CCANNNNNNTGG 1 cut(s) 84
BstYI RGATCY 3 cut(s) 181, 267, 673
BsuRI GGCC 1 cut(s) 622
BtgZI GCGATG 1 cut(s) 81
BtsCI GGATG 2 cut(s) 13, 391
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 171, 841
Cac8I GCNNGC 1 cut(s) 717
CfoI GCGC 2 cut(s) 223, 609
Cfr13I GGNCC 1 cut(s) 620
CviAII CATG 8 cut(s) 42, 53, 128, 280, 469, 491, 527, 829
DdeI CTNAG 4 cut(s) 155, 396, 414, 627
DinI GGCGCC 1 cut(s) 222
DpnI GATC 5 cut(s) 57, 183, 269, 566, 675
DpnII GATC 5 cut(s) 55, 181, 267, 564, 673
DraI TTTAAA 1 cut(s) 577
Eam1104I CTCTTC 2 cut(s) 393, 780
EarI CTCTTC 2 cut(s) 393, 780
EciI GGCGGA 2 cut(s) 20, 174
Ecl136II GAGCTC 2 cut(s) 524, 649
Eco130I CCWWGG 2 cut(s) 77, 616
Eco24I GRGCYC 2 cut(s) 526, 651
Eco47III AGCGCT 1 cut(s) 608
Eco53kI GAGCTC 2 cut(s) 524, 649
Eco57I CTGAAG 2 cut(s) 200, 425
EcoICRI GAGCTC 2 cut(s) 524, 649
EcoT14I CCWWGG 2 cut(s) 77, 616
EcoT38I GRGCYC 2 cut(s) 526, 651
EgeI GGCGCC 1 cut(s) 222
EheI GGCGCC 1 cut(s) 222
ErhI CCWWGG 2 cut(s) 77, 616
FaeI CATG 8 cut(s) 45, 56, 131, 283, 472, 494, 530, 832
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 8 cut(s) 41, 52, 127, 279, 468, 490, 526, 828
FbaI TGATCA 1 cut(s) 55
Fnu4HI GCNGC 8 cut(s) 188, 379, 541, 550, 610, 613, 726, 747
FokI GGATG 2 cut(s) 20, 378
FriOI GRGCYC 2 cut(s) 526, 651
Fsp4HI GCNGC 8 cut(s) 188, 379, 541, 550, 610, 613, 726, 747
GlaI GCGC 2 cut(s) 222, 608
GluI GCNGC 8 cut(s) 188, 379, 541, 550, 610, 613, 726, 747
HaeII RGCGCY 2 cut(s) 224, 610
HaeIII GGCC 1 cut(s) 622
HapII CCGG 4 cut(s) 224, 330, 366, 840
HhaI GCGC 2 cut(s) 223, 609
Hin1I GRCGYC 1 cut(s) 221
Hin1II CATG 8 cut(s) 45, 56, 131, 283, 472, 494, 530, 832
Hin6I GCGC 2 cut(s) 221, 607
HinP1I GCGC 2 cut(s) 221, 607
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 4 cut(s) 88, 234, 314, 770
HpaII CCGG 4 cut(s) 224, 330, 366, 840
HphI GGTGA 3 cut(s) 301, 457, 676
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 1 cut(s) 817
Hpy8I GTNNAC 1 cut(s) 448
HpyAV CCTTC 2 cut(s) 511, 795
HpyCH4V TGCA 6 cut(s) 176, 381, 540, 552, 612, 719
HpyF10VI GCNNNNNNNGC 5 cut(s) 220, 546, 549, 662, 725
HpyF3I CTNAG 4 cut(s) 155, 396, 414, 627
Hsp92I GRCGYC 1 cut(s) 221
Hsp92II CATG 8 cut(s) 45, 56, 131, 283, 472, 494, 530, 832
HspAI GCGC 2 cut(s) 221, 607
KasI GGCGCC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 5 cut(s) 55, 181, 267, 564, 673
LguI GCTCTTC 1 cut(s) 393
LmnI GCTCC 2 cut(s) 521, 654
LpnPI CCDG 9 cut(s) 203, 237, 343, 379, 428, 438, 615, 729, 789
Lsp1109I GCAGC 7 cut(s) 365, 536, 552, 596, 624, 737, 758
LweI GCATC 1 cut(s) 571
MaeIII GTNAC 1 cut(s) 289
MalI GATC 5 cut(s) 57, 183, 269, 566, 675
MboI GATC 5 cut(s) 55, 181, 267, 564, 673
MboII GAAGA 4 cut(s) 349, 380, 431, 767
MflI RGATCY 3 cut(s) 181, 267, 673
MhlI GDGCHC 2 cut(s) 526, 651
MluCI AATT 5 cut(s) 191, 205, 572, 680, 707
Mly113I GGCGCC 1 cut(s) 221
MmeI TCCRAC 2 cut(s) 295, 615
MnlI CCTC 9 cut(s) 280, 329, 448, 491, 512, 631, 662, 789, 811
MroXI GAANNNNTTC 1 cut(s) 774
MseI TTAA 3 cut(s) 204, 576, 678
MslI CAYNNNNRTG 3 cut(s) 51, 68, 531
MspI CCGG 4 cut(s) 224, 330, 366, 840
MspR9I CCNGG 3 cut(s) 225, 366, 841
MwoI GCNNNNNNNGC 5 cut(s) 220, 546, 549, 662, 725
NarI GGCGCC 1 cut(s) 221
NciI CCSGG 3 cut(s) 225, 366, 841
NdeII GATC 5 cut(s) 55, 181, 267, 564, 673
NlaIII CATG 8 cut(s) 45, 56, 131, 283, 472, 494, 530, 832
NlaIV GGNNCC 3 cut(s) 183, 222, 269
NmuCI GTSAC 1 cut(s) 289
NspV TTCGAA 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 393
PdmI GAANNNNTTC 1 cut(s) 774
PfeI GAWTC 4 cut(s) 88, 234, 314, 770
PkrI GCNGC 8 cut(s) 189, 380, 542, 551, 611, 614, 727, 748
PluTI GGCGCC 1 cut(s) 224
PshBI ATTAAT 1 cut(s) 204
Psp124BI GAGCTC 2 cut(s) 526, 651
PspN4I GGNNCC 3 cut(s) 183, 222, 269
PspPI GGNCC 1 cut(s) 620
PstI CTGCAG 1 cut(s) 614
PsuI RGATCY 3 cut(s) 181, 267, 673
RseI CAYNNNNRTG 3 cut(s) 51, 68, 531
SacI GAGCTC 2 cut(s) 526, 651
SapI GCTCTTC 1 cut(s) 393
SaqAI TTAA 3 cut(s) 204, 576, 678
SatI GCNGC 8 cut(s) 188, 379, 541, 550, 610, 613, 726, 747
Sau3AI GATC 5 cut(s) 55, 181, 267, 564, 673
Sau96I GGNCC 1 cut(s) 620
ScrFI CCNGG 3 cut(s) 225, 366, 841
SduI GDGCHC 2 cut(s) 526, 651
SfaNI GCATC 1 cut(s) 571
SfcI CTRYAG 2 cut(s) 514, 610
SfoI GGCGCC 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 232
SmiMI CAYNNNNRTG 3 cut(s) 51, 68, 531
Sse9I AATT 5 cut(s) 191, 205, 572, 680, 707
SsiI CCGC 3 cut(s) 5, 185, 188
SspDI GGCGCC 1 cut(s) 220
SstI GAGCTC 2 cut(s) 526, 651
StyD4I CCNGG 3 cut(s) 223, 364, 839
StyI CCWWGG 2 cut(s) 77, 616
TaqI TCGA 1 cut(s) 232
TasI AATT 5 cut(s) 191, 205, 572, 680, 707
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 4 cut(s) 88, 234, 314, 770
Tru1I TTAA 3 cut(s) 204, 576, 678
Tru9I TTAA 3 cut(s) 204, 576, 678
TscAI CASTG 2 cut(s) 178, 841
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 7 cut(s) 378, 540, 549, 609, 612, 725, 746
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 7 cut(s) 27, 116, 296, 380, 434, 479, 747
TspGWI ACGGA 2 cut(s) 134, 151
TspRI CASTG 2 cut(s) 178, 841
VspI ATTAAT 1 cut(s) 204
XapI RAATTY 3 cut(s) 572, 680, 707
XmnI GAANNNNTTC 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.