RchiOBHm_Chr3g0497211

Thioredoxin-like 1-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0006818
Physical Location & Seq
Forward (+)
48915209 .. 48917450
2242 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45942

Sequence Viewer

Length: 849 bp
ATGGCGGATGTGGTTGTGAGCAAAACCAACTTGTTTTCATCATGCCACCATCATCATCAACACATCTCTGTGTTTCCAAAGACTTGGAATCAATCATCGTTGTCATTCTCATCAAAGACGGACTTTCATGGCAAACGGATTGGGCTTCAACAACTTAGACGACCCCTTCCCTCTGCAAGGGATCCGCCGCAATTCCAAGCCATTAATTCACAGCTTCAGGCGCCGGGTTTTCGAATCGGTAAAGCTCATAGATGGTGGGAAAAGGGGATCCAACCCAACATGAAAGAGGTGACGTCAGCACAAGACCTTGTGGATTCCCTCTCAAACGCAGGAGATAAGCTTGTCGTCCTTGATTTCTTCTCTCCCGGTTGTGGTGGCTGCAAAGCTCTTCATCCCAAGATATGTCAGTTCGCTGATATGAACCCAGATGTTCAGTTTCTTCAGGTGAACTATGAGGAACACAAATCCATGTGTTATAGCCTCAATGTTCATGTCCTACCCTTCTTTCGCTTCTACAGAGGAGCTCATGGTCGTGTATGCAGCTTTAGCTGCACAAACGCCACGATCAAAAAATTTAAAGATGCGTTAGCCAAGCACAAGCCAGAGCGCTGCAGCCTTGGGCCAACTAAGGGGTTGGAGGAGAAAGAGCTCCTCGCACTTTCTGCTAATAAAGATCTTAAATTCACCTACACCCCTAAACAAGTTGAATTTGTGCCTGCAAAAGCATCAGAGGAAATGATTGTGGCAGCACCATCTCGTTCAAACTCGGAATCTCTTCCTCTACCTTCAACAACTTTGAAGTCTGGTCAAATCTCCGAGGAGAGAAACATGGTCAGTGCTGGGAGATGA

Protein Analysis

282

Amino Acids

31.5

Weight (kDa)

9.31

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 107 - 176 1.8e-12 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 220
AciI CCGC 3 cut(s) 5, 185, 188
AclWI GGATC 4 cut(s) 176, 189, 262, 275
AcsI RAATTY 3 cut(s) 572, 680, 707
AcuI CTGAAG 2 cut(s) 200, 425
AcyI GRCGYC 2 cut(s) 221, 293
AfeI AGCGCT 1 cut(s) 608
AfiI CCNNNNNNNGG 3 cut(s) 177, 371, 629
AgsI TTSAA 5 cut(s) 149, 707, 762, 789, 799
AluBI AGCT 8 cut(s) 214, 245, 340, 386, 524, 543, 549, 649
AluI AGCT 8 cut(s) 214, 245, 340, 386, 524, 543, 549, 649
Alw21I GWGCWC 2 cut(s) 526, 651
AlwI GGATC 4 cut(s) 176, 189, 262, 275
Aor51HI AGCGCT 1 cut(s) 608
AoxI GGCC 1 cut(s) 620
ApeKI GCWGC 6 cut(s) 378, 540, 549, 609, 612, 746
ApoI RAATTY 3 cut(s) 572, 680, 707
AseI ATTAAT 1 cut(s) 204
Asp700I GAANNNNTTC 1 cut(s) 774
AspLEI GCGC 2 cut(s) 223, 609
AspS9I GGNCC 1 cut(s) 620
AsuC2I CCSGG 2 cut(s) 225, 366
AsuHPI GGTGA 3 cut(s) 301, 457, 676
AsuII TTCGAA 1 cut(s) 232
BamHI GGATCC 2 cut(s) 181, 267
BanI GGYRCC 1 cut(s) 220
BanII GRGCYC 2 cut(s) 526, 651
Bbv12I GWGCWC 2 cut(s) 526, 651
BbvI GCAGC 6 cut(s) 365, 536, 552, 596, 624, 758
BccI CCATC 3 cut(s) 57, 246, 760
BcnI CCSGG 2 cut(s) 225, 366
BfmI CTRYAG 2 cut(s) 514, 610
BfoI RGCGCY 2 cut(s) 224, 610
BglII AGATCT 1 cut(s) 673
BisI GCNGC 7 cut(s) 188, 379, 541, 550, 610, 613, 747
BlsI GCNGC 7 cut(s) 189, 380, 542, 551, 611, 614, 748
Bme1390I CCNGG 2 cut(s) 225, 366
BmgT120I GGNCC 1 cut(s) 620
BmiI GGNNCC 3 cut(s) 183, 222, 269
BmrFI CCNGG 2 cut(s) 225, 366
BmsI GCATC 2 cut(s) 571, 734
Bpu14I TTCGAA 1 cut(s) 232
BpuMI CCSGG 2 cut(s) 225, 366
BsaHI GRCGYC 2 cut(s) 221, 293
BsaJI CCNNGG 2 cut(s) 616, 816
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 371, 629
BseDI CCNNGG 2 cut(s) 616, 816
BseGI GGATG 2 cut(s) 13, 391
BseLI CCNNNNNNNGG 3 cut(s) 177, 371, 629
BseRI GAGGAG 4 cut(s) 534, 641, 653, 833
BseXI GCAGC 6 cut(s) 365, 536, 552, 596, 624, 758
BseYI CCCAGC 1 cut(s) 839
BsgI GTGCAG 1 cut(s) 535
BshFI GGCC 1 cut(s) 622
BshNI GGYRCC 1 cut(s) 220
BsiHKAI GWGCWC 2 cut(s) 526, 651
BsiSI CCGG 2 cut(s) 224, 366
BslI CCNNNNNNNGG 3 cut(s) 177, 371, 629
BsnI GGCC 1 cut(s) 622
Bsp119I TTCGAA 1 cut(s) 232
Bsp1286I GDGCHC 2 cut(s) 526, 651
Bsp143I GATC 4 cut(s) 181, 267, 564, 673
BspACI CCGC 3 cut(s) 5, 185, 188
BspANI GGCC 1 cut(s) 622
BspLI GGNNCC 3 cut(s) 183, 222, 269
BspMAI CTGCAG 1 cut(s) 614
BspPI GGATC 4 cut(s) 176, 189, 262, 275
BspQI GCTCTTC 1 cut(s) 393
BspT104I TTCGAA 1 cut(s) 232
BspT107I GGYRCC 1 cut(s) 220
BssECI CCNNGG 2 cut(s) 616, 816
BssMI GATC 4 cut(s) 181, 267, 564, 673
BssNI GRCGYC 2 cut(s) 221, 293
BssT1I CCWWGG 1 cut(s) 616
Bst6I CTCTTC 2 cut(s) 393, 780
BstACI GRCGYC 2 cut(s) 221, 293
BstAPI GCANNNNNTGC 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 232
BstC8I GCNNGC 1 cut(s) 717
BstDEI CTNAG 2 cut(s) 155, 627
BstENI CCTNNNNNAGG 1 cut(s) 175
BstF5I GGATG 2 cut(s) 13, 391
BstH2I RGCGCY 2 cut(s) 224, 610
BstHHI GCGC 2 cut(s) 223, 609
BstKTI GATC 4 cut(s) 184, 270, 567, 676
BstMBI GATC 4 cut(s) 181, 267, 564, 673
BstMWI GCNNNNNNNGC 4 cut(s) 220, 546, 549, 662
BstSCI CCNGG 2 cut(s) 223, 364
BstSFI CTRYAG 2 cut(s) 514, 610
BstV1I GCAGC 6 cut(s) 365, 536, 552, 596, 624, 758
BstX2I RGATCY 3 cut(s) 181, 267, 673
BstXI CCANNNNNNTGG 1 cut(s) 84
BstYI RGATCY 3 cut(s) 181, 267, 673
BsuRI GGCC 1 cut(s) 622
BtsCI GGATG 2 cut(s) 13, 391
BtsIMutI CAGTG 1 cut(s) 841
Cac8I GCNNGC 1 cut(s) 717
CfoI GCGC 2 cut(s) 223, 609
Cfr13I GGNCC 1 cut(s) 620
CviAII CATG 7 cut(s) 42, 128, 280, 469, 491, 527, 829
DdeI CTNAG 2 cut(s) 155, 627
DinI GGCGCC 1 cut(s) 222
DpnI GATC 4 cut(s) 183, 269, 566, 675
DpnII GATC 4 cut(s) 181, 267, 564, 673
DraI TTTAAA 1 cut(s) 577
Eam1104I CTCTTC 2 cut(s) 393, 780
EarI CTCTTC 2 cut(s) 393, 780
EciI GGCGGA 2 cut(s) 20, 174
Ecl136II GAGCTC 2 cut(s) 524, 649
Eco130I CCWWGG 1 cut(s) 616
Eco24I GRGCYC 2 cut(s) 526, 651
Eco47III AGCGCT 1 cut(s) 608
Eco53kI GAGCTC 2 cut(s) 524, 649
Eco57I CTGAAG 2 cut(s) 200, 425
EcoICRI GAGCTC 2 cut(s) 524, 649
EcoNI CCTNNNNNAGG 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 616
EcoT38I GRGCYC 2 cut(s) 526, 651
EgeI GGCGCC 1 cut(s) 222
EheI GGCGCC 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 616
FaeI CATG 7 cut(s) 45, 131, 283, 472, 494, 530, 832
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 7 cut(s) 41, 127, 279, 468, 490, 526, 828
Fnu4HI GCNGC 7 cut(s) 188, 379, 541, 550, 610, 613, 747
FokI GGATG 2 cut(s) 20, 378
FriOI GRGCYC 2 cut(s) 526, 651
Fsp4HI GCNGC 7 cut(s) 188, 379, 541, 550, 610, 613, 747
GlaI GCGC 2 cut(s) 222, 608
GluI GCNGC 7 cut(s) 188, 379, 541, 550, 610, 613, 747
GsaI CCCAGC 1 cut(s) 843
HaeII RGCGCY 2 cut(s) 224, 610
HaeIII GGCC 1 cut(s) 622
HapII CCGG 2 cut(s) 224, 366
HhaI GCGC 2 cut(s) 223, 609
Hin1I GRCGYC 2 cut(s) 221, 293
Hin1II CATG 7 cut(s) 45, 131, 283, 472, 494, 530, 832
Hin6I GCGC 2 cut(s) 221, 607
HinP1I GCGC 2 cut(s) 221, 607
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 4 cut(s) 88, 234, 314, 770
HpaII CCGG 2 cut(s) 224, 366
HphI GGTGA 3 cut(s) 301, 457, 676
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 3 cut(s) 730, 769, 817
Hpy8I GTNNAC 1 cut(s) 448
HpyAV CCTTC 3 cut(s) 176, 511, 795
HpyCH4IV ACGT 1 cut(s) 293
HpyCH4V TGCA 6 cut(s) 176, 381, 540, 552, 612, 719
HpyF10VI GCNNNNNNNGC 4 cut(s) 220, 546, 549, 662
HpyF3I CTNAG 2 cut(s) 155, 627
HpySE526I ACGT 1 cut(s) 293
Hsp92I GRCGYC 2 cut(s) 221, 293
Hsp92II CATG 7 cut(s) 45, 131, 283, 472, 494, 530, 832
HspAI GCGC 2 cut(s) 221, 607
KasI GGCGCC 1 cut(s) 220
Kzo9I GATC 4 cut(s) 181, 267, 564, 673
LguI GCTCTTC 1 cut(s) 393
LmnI GCTCC 2 cut(s) 521, 654
Lsp1109I GCAGC 6 cut(s) 365, 536, 552, 596, 624, 758
LweI GCATC 2 cut(s) 571, 734
MaeII ACGT 1 cut(s) 293
MaeIII GTNAC 1 cut(s) 289
MalI GATC 4 cut(s) 183, 269, 566, 675
MboI GATC 4 cut(s) 181, 267, 564, 673
MboII GAAGA 4 cut(s) 349, 380, 431, 767
MflI RGATCY 3 cut(s) 181, 267, 673
MhlI GDGCHC 2 cut(s) 526, 651
MluCI AATT 5 cut(s) 191, 205, 572, 680, 707
Mly113I GGCGCC 1 cut(s) 221
MmeI TCCRAC 2 cut(s) 295, 615
MroXI GAANNNNTTC 1 cut(s) 774
MseI TTAA 3 cut(s) 204, 576, 678
MslI CAYNNNNRTG 2 cut(s) 68, 531
MspI CCGG 2 cut(s) 224, 366
MspR9I CCNGG 2 cut(s) 225, 366
MwoI GCNNNNNNNGC 4 cut(s) 220, 546, 549, 662
NarI GGCGCC 1 cut(s) 221
NciI CCSGG 2 cut(s) 225, 366
NdeII GATC 4 cut(s) 181, 267, 564, 673
NlaIII CATG 7 cut(s) 45, 131, 283, 472, 494, 530, 832
NlaIV GGNNCC 3 cut(s) 183, 222, 269
NmuCI GTSAC 1 cut(s) 289
NspV TTCGAA 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 393
PdmI GAANNNNTTC 1 cut(s) 774
PfeI GAWTC 4 cut(s) 88, 234, 314, 770
PkrI GCNGC 7 cut(s) 189, 380, 542, 551, 611, 614, 748
PluTI GGCGCC 1 cut(s) 224
PshBI ATTAAT 1 cut(s) 204
Psp124BI GAGCTC 2 cut(s) 526, 651
PspFI CCCAGC 1 cut(s) 839
PspN4I GGNNCC 3 cut(s) 183, 222, 269
PspPI GGNCC 1 cut(s) 620
PstI CTGCAG 1 cut(s) 614
PsuI RGATCY 3 cut(s) 181, 267, 673
RseI CAYNNNNRTG 2 cut(s) 68, 531
SacI GAGCTC 2 cut(s) 526, 651
SapI GCTCTTC 1 cut(s) 393
SaqAI TTAA 3 cut(s) 204, 576, 678
SatI GCNGC 7 cut(s) 188, 379, 541, 550, 610, 613, 747
Sau3AI GATC 4 cut(s) 181, 267, 564, 673
Sau96I GGNCC 1 cut(s) 620
ScrFI CCNGG 2 cut(s) 225, 366
SduI GDGCHC 2 cut(s) 526, 651
SfaNI GCATC 2 cut(s) 571, 734
SfcI CTRYAG 2 cut(s) 514, 610
SfoI GGCGCC 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 232
SmiMI CAYNNNNRTG 2 cut(s) 68, 531
Sse9I AATT 5 cut(s) 191, 205, 572, 680, 707
SsiI CCGC 3 cut(s) 5, 185, 188
SspDI GGCGCC 1 cut(s) 220
SstI GAGCTC 2 cut(s) 526, 651
StyD4I CCNGG 2 cut(s) 223, 364
StyI CCWWGG 1 cut(s) 616
TaiI ACGT 1 cut(s) 296
TaqI TCGA 1 cut(s) 232
TasI AATT 5 cut(s) 191, 205, 572, 680, 707
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 4 cut(s) 88, 234, 314, 770
Tru1I TTAA 3 cut(s) 204, 576, 678
Tru9I TTAA 3 cut(s) 204, 576, 678
TscAI CASTG 1 cut(s) 841
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 6 cut(s) 378, 540, 549, 609, 612, 746
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 6 cut(s) 27, 116, 296, 380, 434, 479
TspGWI ACGGA 2 cut(s) 134, 151
TspRI CASTG 1 cut(s) 841
VspI ATTAAT 1 cut(s) 204
XagI CCTNNNNNAGG 1 cut(s) 175
XapI RAATTY 3 cut(s) 572, 680, 707
XmnI GAANNNNTTC 1 cut(s) 774
ZraI GACGTC 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.