Rw0G003520

Thioredoxin-like 1-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00125
OG0006818
Physical Location & Seq
Forward (+)
4889 .. 6378
1490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G003520.1

Sequence Viewer

Length: 858 bp
ATGGCGGATGTGGTTGTGAGCAAAACCAACTTGTTTTCATCATGCCACCACCATCATCAACACATCTCTGTGTTTCCAAAGACTTGGAATCAATCATCGCTGTCATTCTCATCAAAGACGGACTTTCACGGCAAACGGATTGGGCTTCAACAACTTAGACGACCCTTTCCCACTGCAAGGGATCCGCCGCAATTCCAAGCCATTAATTCACAGCTTCAGGCGCCGGGTTTTCGAATCGGTAAAGCTCATAGATGGTGGGAAAAGGGGATCCAACCCAACATGAAAGAGGTGACGTCAGCACAAGACCTTGTGGATTCCCTCTCAAACGCAGGAGATAAGCTTGTCGTCCTTGATTTCTTCTCTCCCGGTTGTGGTGGCTGCAAGGCTCTTCATCCCAAGATATGTCAGTTCGCTGAGATGAACCCAGATGTTCAGTTTCTTCAGGTGAACTATGAGGAACACAAATCCATGTGTTATAGCCTCAATGTTCATGTCCTACCCTTCTTTCGCTTCTACAGAGGAGCTCATGGTCGTGTATGCAGCTTTAGCTGCACAAACGCCACGATAAAAAAATTTAAAGATGCGTTAGCCAAGCACAAGCCAGAGCGCTGCAGCCTTGGGCCAACTAAGGGGTTGGAGGAGAAAGAGCTCCTCGCACTTTCTGCACTTTCTGCTAATAAAGATCTTAAATTCACCTACACCCCTAAACAAGTTGAATTTGTGCCTGCAAAAGCAGCAGATGAAATGATTGTGGCAGCACCATCTCGTTCAAACTTGGAATCTCTTCCTCTGCCTTCAACAACATTGAAGTCTGGTCAAATCTCCGAGGAGAGAAACATGGTCAGTGCTGGGAGATGA

Protein Analysis

285

Amino Acids

31.83

Weight (kDa)

9.31

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 107 - 176 1.3e-12 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 220
AciI CCGC 3 cut(s) 5, 185, 188
AclWI GGATC 4 cut(s) 176, 189, 262, 275
AcsI RAATTY 3 cut(s) 572, 689, 716
AcuI CTGAAG 2 cut(s) 200, 425
AcyI GRCGYC 2 cut(s) 221, 293
AfeI AGCGCT 1 cut(s) 608
AfiI CCNNNNNNNGG 3 cut(s) 177, 371, 629
AgsI TTSAA 5 cut(s) 149, 716, 771, 798, 808
AluBI AGCT 7 cut(s) 214, 245, 340, 524, 543, 549, 649
AluI AGCT 7 cut(s) 214, 245, 340, 524, 543, 549, 649
Alw21I GWGCWC 2 cut(s) 526, 651
AlwI GGATC 4 cut(s) 176, 189, 262, 275
Aor51HI AGCGCT 1 cut(s) 608
AoxI GGCC 1 cut(s) 620
ApeKI GCWGC 7 cut(s) 378, 540, 549, 609, 612, 734, 755
ApoI RAATTY 3 cut(s) 572, 689, 716
AseI ATTAAT 1 cut(s) 204
Asp700I GAANNNNTTC 1 cut(s) 783
AspLEI GCGC 2 cut(s) 223, 609
AspS9I GGNCC 1 cut(s) 620
AsuC2I CCSGG 2 cut(s) 225, 366
AsuHPI GGTGA 3 cut(s) 301, 457, 685
AsuII TTCGAA 1 cut(s) 232
BamHI GGATCC 2 cut(s) 181, 267
BanI GGYRCC 1 cut(s) 220
BanII GRGCYC 2 cut(s) 526, 651
Bbv12I GWGCWC 2 cut(s) 526, 651
BbvI GCAGC 7 cut(s) 365, 536, 552, 596, 624, 746, 767
BccI CCATC 3 cut(s) 60, 246, 769
BceAI ACGGC 1 cut(s) 145
BcnI CCSGG 2 cut(s) 225, 366
BfmI CTRYAG 2 cut(s) 514, 610
BfoI RGCGCY 2 cut(s) 224, 610
BglII AGATCT 1 cut(s) 682
BisI GCNGC 8 cut(s) 188, 379, 541, 550, 610, 613, 735, 756
BlsI GCNGC 8 cut(s) 189, 380, 542, 551, 611, 614, 736, 757
Bme1390I CCNGG 2 cut(s) 225, 366
BmgT120I GGNCC 1 cut(s) 620
BmiI GGNNCC 3 cut(s) 183, 222, 269
BmrFI CCNGG 2 cut(s) 225, 366
BmsI GCATC 1 cut(s) 571
Bpu14I TTCGAA 1 cut(s) 232
BpuMI CCSGG 2 cut(s) 225, 366
BsaHI GRCGYC 2 cut(s) 221, 293
BsaJI CCNNGG 2 cut(s) 616, 825
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 371, 629
BseDI CCNNGG 2 cut(s) 616, 825
BseGI GGATG 2 cut(s) 13, 391
BseLI CCNNNNNNNGG 3 cut(s) 177, 371, 629
BseMII CTCAG 1 cut(s) 405
BseRI GAGGAG 4 cut(s) 534, 641, 653, 842
BseXI GCAGC 7 cut(s) 365, 536, 552, 596, 624, 746, 767
BseYI CCCAGC 1 cut(s) 848
BsgI GTGCAG 2 cut(s) 535, 648
BshFI GGCC 1 cut(s) 622
BshNI GGYRCC 1 cut(s) 220
BsiHKAI GWGCWC 2 cut(s) 526, 651
BsiSI CCGG 2 cut(s) 224, 366
BslI CCNNNNNNNGG 3 cut(s) 177, 371, 629
BsnI GGCC 1 cut(s) 622
Bsp119I TTCGAA 1 cut(s) 232
Bsp1286I GDGCHC 2 cut(s) 526, 651
Bsp143I GATC 3 cut(s) 181, 267, 682
BspACI CCGC 3 cut(s) 5, 185, 188
BspANI GGCC 1 cut(s) 622
BspCNI CTCAG 1 cut(s) 406
BspLI GGNNCC 3 cut(s) 183, 222, 269
BspMAI CTGCAG 1 cut(s) 614
BspPI GGATC 4 cut(s) 176, 189, 262, 275
BspQI GCTCTTC 1 cut(s) 393
BspT104I TTCGAA 1 cut(s) 232
BspT107I GGYRCC 1 cut(s) 220
BssECI CCNNGG 2 cut(s) 616, 825
BssMI GATC 3 cut(s) 181, 267, 682
BssNI GRCGYC 2 cut(s) 221, 293
BssT1I CCWWGG 1 cut(s) 616
Bst6I CTCTTC 2 cut(s) 393, 789
BstACI GRCGYC 2 cut(s) 221, 293
BstAPI GCANNNNNTGC 2 cut(s) 662, 671
BstBI TTCGAA 1 cut(s) 232
BstC8I GCNNGC 1 cut(s) 726
BstDEI CTNAG 3 cut(s) 155, 414, 627
BstF5I GGATG 2 cut(s) 13, 391
BstH2I RGCGCY 2 cut(s) 224, 610
BstHHI GCGC 2 cut(s) 223, 609
BstKTI GATC 3 cut(s) 184, 270, 685
BstMBI GATC 3 cut(s) 181, 267, 682
BstMWI GCNNNNNNNGC 6 cut(s) 220, 546, 549, 662, 671, 734
BstSCI CCNGG 2 cut(s) 223, 364
BstSFI CTRYAG 2 cut(s) 514, 610
BstV1I GCAGC 7 cut(s) 365, 536, 552, 596, 624, 746, 767
BstX2I RGATCY 3 cut(s) 181, 267, 682
BstXI CCANNNNNNTGG 1 cut(s) 84
BstYI RGATCY 3 cut(s) 181, 267, 682
BsuRI GGCC 1 cut(s) 622
BtgZI GCGATG 1 cut(s) 81
BtsCI GGATG 2 cut(s) 13, 391
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 171, 850
Cac8I GCNNGC 1 cut(s) 726
CfoI GCGC 2 cut(s) 223, 609
Cfr13I GGNCC 1 cut(s) 620
CviAII CATG 6 cut(s) 42, 280, 469, 491, 527, 838
DdeI CTNAG 3 cut(s) 155, 414, 627
DinI GGCGCC 1 cut(s) 222
DpnI GATC 3 cut(s) 183, 269, 684
DpnII GATC 3 cut(s) 181, 267, 682
DraI TTTAAA 1 cut(s) 577
Eam1104I CTCTTC 2 cut(s) 393, 789
EarI CTCTTC 2 cut(s) 393, 789
EciI GGCGGA 2 cut(s) 20, 174
Ecl136II GAGCTC 2 cut(s) 524, 649
Eco130I CCWWGG 1 cut(s) 616
Eco24I GRGCYC 2 cut(s) 526, 651
Eco47III AGCGCT 1 cut(s) 608
Eco53kI GAGCTC 2 cut(s) 524, 649
Eco57I CTGAAG 2 cut(s) 200, 425
EcoICRI GAGCTC 2 cut(s) 524, 649
EcoT14I CCWWGG 1 cut(s) 616
EcoT38I GRGCYC 2 cut(s) 526, 651
EgeI GGCGCC 1 cut(s) 222
EheI GGCGCC 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 616
FaeI CATG 6 cut(s) 45, 283, 472, 494, 530, 841
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 6 cut(s) 41, 279, 468, 490, 526, 837
Fnu4HI GCNGC 8 cut(s) 188, 379, 541, 550, 610, 613, 735, 756
FokI GGATG 2 cut(s) 20, 378
FriOI GRGCYC 2 cut(s) 526, 651
Fsp4HI GCNGC 8 cut(s) 188, 379, 541, 550, 610, 613, 735, 756
GlaI GCGC 2 cut(s) 222, 608
GluI GCNGC 8 cut(s) 188, 379, 541, 550, 610, 613, 735, 756
GsaI CCCAGC 1 cut(s) 852
HaeII RGCGCY 2 cut(s) 224, 610
HaeIII GGCC 1 cut(s) 622
HapII CCGG 2 cut(s) 224, 366
HhaI GCGC 2 cut(s) 223, 609
Hin1I GRCGYC 2 cut(s) 221, 293
Hin1II CATG 6 cut(s) 45, 283, 472, 494, 530, 841
Hin6I GCGC 2 cut(s) 221, 607
HinP1I GCGC 2 cut(s) 221, 607
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 4 cut(s) 88, 234, 314, 779
HpaII CCGG 2 cut(s) 224, 366
HphI GGTGA 3 cut(s) 301, 457, 685
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 1 cut(s) 826
Hpy8I GTNNAC 1 cut(s) 448
HpyAV CCTTC 2 cut(s) 511, 804
HpyCH4IV ACGT 1 cut(s) 293
HpyCH4V TGCA 7 cut(s) 176, 381, 540, 552, 612, 665, 728
HpyF10VI GCNNNNNNNGC 6 cut(s) 220, 546, 549, 662, 671, 734
HpyF3I CTNAG 3 cut(s) 155, 414, 627
HpySE526I ACGT 1 cut(s) 293
Hsp92I GRCGYC 2 cut(s) 221, 293
Hsp92II CATG 6 cut(s) 45, 283, 472, 494, 530, 841
HspAI GCGC 2 cut(s) 221, 607
KasI GGCGCC 1 cut(s) 220
Kzo9I GATC 3 cut(s) 181, 267, 682
LguI GCTCTTC 1 cut(s) 393
LmnI GCTCC 2 cut(s) 521, 654
Lsp1109I GCAGC 7 cut(s) 365, 536, 552, 596, 624, 746, 767
LweI GCATC 1 cut(s) 571
MaeII ACGT 1 cut(s) 293
MaeIII GTNAC 1 cut(s) 289
MalI GATC 3 cut(s) 183, 269, 684
MboI GATC 3 cut(s) 181, 267, 682
MboII GAAGA 4 cut(s) 349, 380, 431, 776
MflI RGATCY 3 cut(s) 181, 267, 682
MhlI GDGCHC 2 cut(s) 526, 651
MluCI AATT 5 cut(s) 191, 205, 572, 689, 716
Mly113I GGCGCC 1 cut(s) 221
MmeI TCCRAC 2 cut(s) 295, 615
MnlI CCTC 9 cut(s) 280, 329, 448, 491, 512, 631, 662, 798, 820
MroXI GAANNNNTTC 1 cut(s) 783
MseI TTAA 3 cut(s) 204, 576, 687
MslI CAYNNNNRTG 2 cut(s) 68, 531
MspI CCGG 2 cut(s) 224, 366
MspR9I CCNGG 2 cut(s) 225, 366
MwoI GCNNNNNNNGC 6 cut(s) 220, 546, 549, 662, 671, 734
NarI GGCGCC 1 cut(s) 221
NciI CCSGG 2 cut(s) 225, 366
NdeII GATC 3 cut(s) 181, 267, 682
NlaIII CATG 6 cut(s) 45, 283, 472, 494, 530, 841
NlaIV GGNNCC 3 cut(s) 183, 222, 269
NmuCI GTSAC 1 cut(s) 289
NspV TTCGAA 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 393
PdmI GAANNNNTTC 1 cut(s) 783
PfeI GAWTC 4 cut(s) 88, 234, 314, 779
PkrI GCNGC 8 cut(s) 189, 380, 542, 551, 611, 614, 736, 757
PluTI GGCGCC 1 cut(s) 224
PshBI ATTAAT 1 cut(s) 204
Psp124BI GAGCTC 2 cut(s) 526, 651
PspFI CCCAGC 1 cut(s) 848
PspN4I GGNNCC 3 cut(s) 183, 222, 269
PspPI GGNCC 1 cut(s) 620
PstI CTGCAG 1 cut(s) 614
PsuI RGATCY 3 cut(s) 181, 267, 682
RseI CAYNNNNRTG 2 cut(s) 68, 531
SacI GAGCTC 2 cut(s) 526, 651
SapI GCTCTTC 1 cut(s) 393
SaqAI TTAA 3 cut(s) 204, 576, 687
SatI GCNGC 8 cut(s) 188, 379, 541, 550, 610, 613, 735, 756
Sau3AI GATC 3 cut(s) 181, 267, 682
Sau96I GGNCC 1 cut(s) 620
ScrFI CCNGG 2 cut(s) 225, 366
SduI GDGCHC 2 cut(s) 526, 651
SfaNI GCATC 1 cut(s) 571
SfcI CTRYAG 2 cut(s) 514, 610
SfoI GGCGCC 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 232
SmiMI CAYNNNNRTG 2 cut(s) 68, 531
Sse9I AATT 5 cut(s) 191, 205, 572, 689, 716
SsiI CCGC 3 cut(s) 5, 185, 188
SspDI GGCGCC 1 cut(s) 220
SstI GAGCTC 2 cut(s) 526, 651
StyD4I CCNGG 2 cut(s) 223, 364
StyI CCWWGG 1 cut(s) 616
TaiI ACGT 1 cut(s) 296
TaqI TCGA 1 cut(s) 232
TasI AATT 5 cut(s) 191, 205, 572, 689, 716
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 4 cut(s) 88, 234, 314, 779
Tru1I TTAA 3 cut(s) 204, 576, 687
Tru9I TTAA 3 cut(s) 204, 576, 687
TscAI CASTG 2 cut(s) 178, 850
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 7 cut(s) 378, 540, 549, 609, 612, 734, 755
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 6 cut(s) 27, 296, 380, 434, 479, 756
TspGWI ACGGA 2 cut(s) 134, 151
TspRI CASTG 2 cut(s) 178, 850
VspI ATTAAT 1 cut(s) 204
XapI RAATTY 3 cut(s) 572, 689, 716
XmnI GAANNNNTTC 1 cut(s) 783
ZraI GACGTC 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.