MD14G1033700.v1.1

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003323
Physical Location & Seq
Forward (+)
2986751 .. 2987313
563 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1033700.v1.1.491

Sequence Viewer

Length: 420 bp
ATGGGTCGCGTCCGTACAAAGACAGTGAAGAAATCCTCCCGCCAGGTGATCGAGCGCTACTACTCGCGCATGACCCTGGACTTCCACACCAACAAGAAGATCCTCGAGGAAGTCGCCATCATCCCCTCCAAGCGCCTCCGCAACAAGATTGCCGGATTCTCCACCCACCTCATGAAGCGGATCCAGAAGGGCCCGGTTCGTGGCATTTCGCTGAAGCTGCAGGAGGAGGAGCGCGAGCGCCGCATGGACTTTGTCCCCGAGGAGTCCGCCATCAAGATCGACGAGATCTCCGTGGACAAGGAGACCCTCGACATGCTCTCCGCTCTCGGTATGGCTGACATGCCTGGTGTCAAGCGGGTCGACCCGGAAGCTCAGACTCAGAATCTCGGGTACGGGCGTGGTGCACCCAGGAGGTACTAA

Protein Analysis

140

Amino Acids

16.05

Weight (kDa)

10.03

Isoelectric Point (pI)

52.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S17e PF00833 1 - 120 8.2e-60 Ribosomal S17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 104
AccBSI CCGCTC 1 cut(s) 323
AccI GTMKAC 1 cut(s) 360
AccII CGCG 3 cut(s) 9, 67, 234
AciI CCGC 7 cut(s) 40, 139, 178, 241, 267, 321, 355
AclWI GGATC 3 cut(s) 94, 175, 188
AcuI CTGAAG 1 cut(s) 233
AfaI GTAC 3 cut(s) 16, 392, 416
AfeI AGCGCT 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 200
AjnI CCWGG 4 cut(s) 42, 75, 343, 407
AluBI AGCT 2 cut(s) 217, 371
AluI AGCT 2 cut(s) 217, 371
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 1 cut(s) 296
Alw44I GTGCAC 1 cut(s) 402
AlwI GGATC 3 cut(s) 94, 175, 188
Ama87I CYCGRG 3 cut(s) 104, 257, 386
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 1 cut(s) 190
ApaI GGGCCC 1 cut(s) 194
ApaLI GTGCAC 1 cut(s) 402
ApeKI GCWGC 1 cut(s) 217
AspLEI GCGC 5 cut(s) 57, 69, 135, 234, 240
AspS9I GGNCC 2 cut(s) 190, 191
AsuC2I CCSGG 2 cut(s) 194, 365
AsuHPI GGTGA 1 cut(s) 58
AvaI CYCGRG 3 cut(s) 104, 257, 386
BaeGI GKGCMC 2 cut(s) 194, 406
BaeI ACNNNNGTAYC 2 cut(s) 382, 415
BamHI GGATCC 1 cut(s) 180
BanII GRGCYC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 1 cut(s) 204
BccI CCATC 2 cut(s) 125, 278
BciT130I CCWGG 4 cut(s) 44, 77, 345, 409
BcnI CCSGG 2 cut(s) 194, 365
BcoDI GTCTC 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 218
BfoI RGCGCY 3 cut(s) 58, 136, 241
BglII AGATCT 1 cut(s) 285
BisI GCNGC 2 cut(s) 218, 241
BlsI GCNGC 2 cut(s) 219, 242
Bme1390I CCNGG 6 cut(s) 44, 77, 194, 345, 365, 409
BmeT110I CYCGRG 3 cut(s) 104, 257, 386
BmgT120I GGNCC 2 cut(s) 190, 191
BmiI GGNNCC 2 cut(s) 182, 192
BmrFI CCNGG 6 cut(s) 44, 77, 194, 345, 365, 409
BpuMI CCSGG 2 cut(s) 194, 365
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 4 cut(s) 75, 258, 291, 407
BsaXI ACNNNNNCTCC 4 cut(s) 272, 302, 302, 332
Bsc4I CCNNNNNNNGG 1 cut(s) 200
BseBI CCWGG 4 cut(s) 44, 77, 345, 409
BseDI CCNNGG 4 cut(s) 75, 258, 291, 407
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 1 cut(s) 200
BseMII CTCAG 2 cut(s) 386, 392
BseRI GAGGAG 3 cut(s) 239, 242, 275
BseSI GKGCMC 2 cut(s) 194, 406
BseXI GCAGC 1 cut(s) 204
Bsh1236I CGCG 3 cut(s) 9, 67, 234
BshFI GGCC 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 406
BsiHKCI CYCGRG 3 cut(s) 104, 257, 386
BsiSI CCGG 3 cut(s) 153, 194, 365
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 200
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 239
BsnI GGCC 1 cut(s) 192
Bso31I GGTCTC 1 cut(s) 296
BsoBI CYCGRG 3 cut(s) 104, 257, 386
Bsp120I GGGCCC 1 cut(s) 190
Bsp1286I GDGCHC 2 cut(s) 194, 406
Bsp143I GATC 5 cut(s) 48, 99, 180, 276, 285
BspACI CCGC 7 cut(s) 40, 139, 178, 241, 267, 321, 355
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 2 cut(s) 385, 391
BspFNI CGCG 3 cut(s) 9, 67, 234
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 2 cut(s) 182, 192
BspMAI CTGCAG 1 cut(s) 222
BspPI GGATC 3 cut(s) 94, 175, 188
BspTNI GGTCTC 1 cut(s) 296
BsrBI CCGCTC 1 cut(s) 323
BssECI CCNNGG 4 cut(s) 75, 258, 291, 407
BssMI GATC 5 cut(s) 48, 99, 180, 276, 285
Bst2UI CCWGG 4 cut(s) 44, 77, 345, 409
Bst4CI ACNGT 1 cut(s) 25
BstC8I GCNNGC 1 cut(s) 236
BstDEI CTNAG 2 cut(s) 372, 378
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 3 cut(s) 9, 67, 234
BstH2I RGCGCY 3 cut(s) 58, 136, 241
BstHHI GCGC 5 cut(s) 57, 69, 135, 234, 240
BstKTI GATC 5 cut(s) 51, 102, 183, 279, 288
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 5 cut(s) 48, 99, 180, 276, 285
BstMWI GCNNNNNNNGC 2 cut(s) 217, 240
BstNI CCWGG 4 cut(s) 44, 77, 345, 409
BstNSI RCATGY 2 cut(s) 316, 343
BstSCI CCNGG 6 cut(s) 42, 75, 192, 343, 363, 407
BstSFI CTRYAG 1 cut(s) 218
BstSLI GKGCMC 2 cut(s) 194, 406
BstUI CGCG 3 cut(s) 9, 67, 234
BstV1I GCAGC 1 cut(s) 204
BstX2I RGATCY 3 cut(s) 99, 180, 285
BstYI RGATCY 3 cut(s) 99, 180, 285
BsuRI GGCC 1 cut(s) 192
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 1 cut(s) 236
CciI TCATGA 1 cut(s) 171
CfoI GCGC 5 cut(s) 57, 69, 135, 234, 240
Cfr13I GGNCC 2 cut(s) 190, 191
Csp6I GTAC 3 cut(s) 15, 391, 415
CviAII CATG 5 cut(s) 70, 172, 244, 313, 340
CviJI RGCY 4 cut(s) 192, 217, 335, 371
CviKI_1 RGCY 4 cut(s) 192, 217, 335, 371
CviQI GTAC 3 cut(s) 15, 391, 415
DdeI CTNAG 2 cut(s) 372, 378
DpnI GATC 5 cut(s) 50, 101, 182, 278, 287
DpnII GATC 5 cut(s) 48, 99, 180, 276, 285
EciI GGCGGA 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 296
Eco47III AGCGCT 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 233
Eco88I CYCGRG 3 cut(s) 104, 257, 386
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 4 cut(s) 42, 75, 343, 407
EcoT38I GRGCYC 1 cut(s) 194
FaeI CATG 5 cut(s) 73, 175, 247, 316, 343
FaiI YATR 6 cut(s) 71, 173, 245, 314, 332, 341
FaqI GGGAC 1 cut(s) 239
FatI CATG 5 cut(s) 69, 171, 243, 312, 339
FauI CCCGC 2 cut(s) 47, 348
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 2 cut(s) 218, 241
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 2 cut(s) 218, 241
GlaI GCGC 5 cut(s) 56, 68, 134, 233, 239
GluI GCNGC 2 cut(s) 218, 241
HaeII RGCGCY 3 cut(s) 58, 136, 241
HaeIII GGCC 1 cut(s) 192
HapII CCGG 3 cut(s) 153, 194, 365
HhaI GCGC 5 cut(s) 57, 69, 135, 234, 240
Hin1II CATG 5 cut(s) 73, 175, 247, 316, 343
Hin6I GCGC 5 cut(s) 55, 67, 133, 232, 238
HinP1I GCGC 5 cut(s) 55, 67, 133, 232, 238
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 4 cut(s) 156, 263, 376, 382
HpaII CCGG 3 cut(s) 153, 194, 365
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 3 cut(s) 295, 361, 404
Hpy188I TCNGA 2 cut(s) 375, 381
Hpy188III TCNNGA 3 cut(s) 172, 184, 274
Hpy8I GTNNAC 3 cut(s) 295, 361, 404
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 1 cut(s) 181
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4V TGCA 2 cut(s) 220, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 240
HpyF3I CTNAG 2 cut(s) 372, 378
Hsp92II CATG 5 cut(s) 73, 175, 247, 316, 343
HspAI GCGC 5 cut(s) 55, 67, 133, 232, 238
Kzo9I GATC 5 cut(s) 48, 99, 180, 276, 285
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 1 cut(s) 204
MalI GATC 5 cut(s) 50, 101, 182, 278, 287
MbiI CCGCTC 1 cut(s) 323
MboI GATC 5 cut(s) 48, 99, 180, 276, 285
MboII GAAGA 2 cut(s) 40, 109
MflI RGATCY 3 cut(s) 99, 180, 285
MhlI GDGCHC 2 cut(s) 194, 406
MlyI GAGTC 2 cut(s) 272, 370
MspI CCGG 3 cut(s) 153, 194, 365
MspR9I CCNGG 6 cut(s) 44, 77, 194, 345, 365, 409
MvaI CCWGG 4 cut(s) 44, 77, 345, 409
MvnI CGCG 3 cut(s) 9, 67, 234
MwoI GCNNNNNNNGC 2 cut(s) 217, 240
NciI CCSGG 2 cut(s) 194, 365
NdeII GATC 5 cut(s) 48, 99, 180, 276, 285
NlaIII CATG 5 cut(s) 73, 175, 247, 316, 343
NlaIV GGNNCC 2 cut(s) 182, 192
NspI RCATGY 2 cut(s) 316, 343
PaeR7I CTCGAG 1 cut(s) 104
PagI TCATGA 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PfeI GAWTC 2 cut(s) 156, 382
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 2 cut(s) 219, 242
PleI GAGTC 2 cut(s) 271, 370
PpsI GAGTC 2 cut(s) 271, 370
Psp6I CCWGG 4 cut(s) 42, 75, 343, 407
PspGI CCWGG 4 cut(s) 42, 75, 343, 407
PspN4I GGNNCC 2 cut(s) 182, 192
PspOMI GGGCCC 1 cut(s) 190
PspPI GGNCC 2 cut(s) 190, 191
PspXI VCTCGAGB 1 cut(s) 104
PstI CTGCAG 1 cut(s) 222
PsuI RGATCY 3 cut(s) 99, 180, 285
PsyI GACNNNGTC 1 cut(s) 251
RsaI GTAC 3 cut(s) 16, 392, 416
RsaNI GTAC 3 cut(s) 15, 391, 415
SalI GTCGAC 1 cut(s) 359
SatI GCNGC 2 cut(s) 218, 241
Sau3AI GATC 5 cut(s) 48, 99, 180, 276, 285
Sau96I GGNCC 2 cut(s) 190, 191
SchI GAGTC 2 cut(s) 272, 370
ScrFI CCNGG 6 cut(s) 44, 77, 194, 345, 365, 409
SduI GDGCHC 2 cut(s) 194, 406
SetI ASST 5 cut(s) 48, 171, 219, 373, 416
SfcI CTRYAG 1 cut(s) 218
Sfr274I CTCGAG 1 cut(s) 104
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SsiI CCGC 7 cut(s) 40, 139, 178, 241, 267, 321, 355
StyD4I CCNGG 6 cut(s) 42, 75, 192, 343, 363, 407
TaaI ACNGT 1 cut(s) 25
TaqI TCGA 5 cut(s) 51, 105, 279, 309, 360
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 2 cut(s) 156, 382
TscAI CASTG 1 cut(s) 30
TseI GCWGC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 188
TspGWI ACGGA 1 cut(s) 280
TspRI CASTG 1 cut(s) 30
Tth111I GACNNNGTC 1 cut(s) 251
VneI GTGCAC 1 cut(s) 402
XceI RCATGY 2 cut(s) 316, 343
XhoI CTCGAG 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.