Rh3BG341400

Ribosomal S17

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0003323
Physical Location & Seq
Forward (+)
36669521 .. 36669943
423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG341400.1

Sequence Viewer

Length: 423 bp
ATGGGTCGTGTTCGCACCAAGACAGTGAAGAAGTCCTCACGCCAGGTGATTGAGAGGTACTACTCTAAGATGACCTTGGACTTCCACACTAACAAGAAGATCTTGGAAGAGGTTGCCATCATACCCTCAAAAAGGCTCCGCAACAAGATTGCTGGCTTCTCTACTCACCTGATGAAGCGCATTCAAAAGGGTCCTGTTAGGGGTATCTCGCTGAAGCTGCAGGAAGAAGAGCGTGAGCGTCGCATGGACTTTGTACCTGAGGAGTCTGCTATCAGGATTGATGACATCAAGGTTGATAAGGAGACCCTGGACATGCTTTCTGCTCTTGGCATGTCTGAAATTCCTGGGGTGTCTGAAGCTGAGCCGCAGGTTGTGGTTCCTAGCCAAGGATATGGACGTGGTCCACAGGGAAGGAGGTTTTAA

Protein Analysis

140

Amino Acids

16.06

Weight (kDa)

9.98

Isoelectric Point (pI)

61.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S17e PF00833 1 - 120 2.3e-59 Ribosomal S17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 358
AciI CCGC 2 cut(s) 139, 365
AcsI RAATTY 1 cut(s) 339
AcuI CTGAAG 2 cut(s) 233, 375
AfaI GTAC 2 cut(s) 59, 255
AfiI CCNNNNNNNGG 3 cut(s) 132, 200, 386
AgsI TTSAA 1 cut(s) 185
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 3 cut(s) 42, 306, 343
AluBI AGCT 2 cut(s) 217, 359
AluI AGCT 2 cut(s) 217, 359
Alw26I GTCTC 1 cut(s) 296
ApeKI GCWGC 1 cut(s) 217
ApoI RAATTY 1 cut(s) 339
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 2 cut(s) 191, 401
AsuHPI GGTGA 2 cut(s) 58, 158
AvaII GGWCC 2 cut(s) 191, 401
AxyI CCTNAGG 1 cut(s) 258
BbvI GCAGC 1 cut(s) 204
BccI CCATC 1 cut(s) 125
BciT130I CCWGG 3 cut(s) 44, 308, 345
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 1 cut(s) 218
BfuAI ACCTGC 1 cut(s) 358
BglII AGATCT 1 cut(s) 99
BisI GCNGC 2 cut(s) 218, 365
BlpI GCTNAGC 1 cut(s) 360
BlsI GCNGC 2 cut(s) 219, 366
Bme1390I CCNGG 3 cut(s) 44, 308, 345
Bme18I GGWCC 2 cut(s) 191, 401
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 2 cut(s) 191, 401
BmiI GGNNCC 3 cut(s) 137, 192, 378
BmrFI CCNGG 3 cut(s) 44, 308, 345
Bpu1102I GCTNAGC 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 4 cut(s) 75, 306, 344, 385
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 200, 386
Bse21I CCTNAGG 1 cut(s) 258
BseBI CCWGG 3 cut(s) 44, 308, 345
BseDI CCNNGG 4 cut(s) 75, 306, 344, 385
BseLI CCNNNNNNNGG 3 cut(s) 132, 200, 386
BseMII CTCAG 2 cut(s) 249, 351
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 1 cut(s) 204
BslI CCNNNNNNNGG 3 cut(s) 132, 200, 386
BsmAI GTCTC 1 cut(s) 296
BsmI GAATGC 1 cut(s) 180
Bso31I GGTCTC 1 cut(s) 296
Bsp143I GATC 1 cut(s) 99
Bsp1720I GCTNAGC 1 cut(s) 360
BspACI CCGC 2 cut(s) 139, 365
BspCNI CTCAG 2 cut(s) 250, 352
BspLI GGNNCC 3 cut(s) 137, 192, 378
BspMAI CTGCAG 1 cut(s) 222
BspMI ACCTGC 1 cut(s) 358
BspQI GCTCTTC 1 cut(s) 222
BspTNI GGTCTC 1 cut(s) 296
BssECI CCNNGG 4 cut(s) 75, 306, 344, 385
BssMI GATC 1 cut(s) 99
BssT1I CCWWGG 2 cut(s) 75, 385
Bst2UI CCWGG 3 cut(s) 44, 308, 345
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 2 cut(s) 102, 222
BstC8I GCNNGC 1 cut(s) 154
BstDEI CTNAG 3 cut(s) 66, 258, 360
BstENI CCTNNNNNAGG 2 cut(s) 130, 384
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 1 cut(s) 102
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 1 cut(s) 99
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNI CCWGG 3 cut(s) 44, 308, 345
BstNSI RCATGY 2 cut(s) 316, 334
BstSCI CCNGG 3 cut(s) 42, 306, 343
BstSFI CTRYAG 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 99
BstXI CCANNNNNNTGG 1 cut(s) 392
BstYI RGATCY 1 cut(s) 99
Bsu36I CCTNAGG 1 cut(s) 258
BtrI CACGTC 1 cut(s) 398
BtsIMutI CAGTG 1 cut(s) 30
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 1 cut(s) 154
CfoI GCGC 1 cut(s) 180
Cfr13I GGNCC 2 cut(s) 191, 401
CseI GACGC 1 cut(s) 227
Csp6I GTAC 2 cut(s) 58, 254
CviAII CATG 3 cut(s) 244, 313, 331
CviJI RGCY 6 cut(s) 136, 156, 217, 359, 364, 384
CviKI_1 RGCY 6 cut(s) 136, 156, 217, 359, 364, 384
CviQI GTAC 2 cut(s) 58, 254
DdeI CTNAG 3 cut(s) 66, 258, 360
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
Eam1104I CTCTTC 2 cut(s) 102, 222
EarI CTCTTC 2 cut(s) 102, 222
Eco130I CCWWGG 2 cut(s) 75, 385
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 2 cut(s) 191, 401
Eco57I CTGAAG 2 cut(s) 233, 375
Eco81I CCTNAGG 1 cut(s) 258
EcoNI CCTNNNNNAGG 2 cut(s) 130, 384
EcoO109I RGGNCCY 1 cut(s) 191
EcoRII CCWGG 3 cut(s) 42, 306, 343
EcoT14I CCWWGG 2 cut(s) 75, 385
ErhI CCWWGG 2 cut(s) 75, 385
FaeI CATG 3 cut(s) 247, 316, 334
FaiI YATR 5 cut(s) 122, 245, 314, 332, 393
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 86, 118
FatI CATG 3 cut(s) 243, 312, 330
Fnu4HI GCNGC 2 cut(s) 218, 365
Fsp4HI GCNGC 2 cut(s) 218, 365
FspBI CTAG 1 cut(s) 381
GlaI GCGC 1 cut(s) 179
GluI GCNGC 2 cut(s) 218, 365
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 180
Hin1II CATG 3 cut(s) 247, 316, 334
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HinfI GANTC 1 cut(s) 263
HphI GGTGA 2 cut(s) 58, 158
Hpy166II GTNNAC 1 cut(s) 404
Hpy188I TCNGA 2 cut(s) 337, 355
Hpy188III TCNNGA 1 cut(s) 274
Hpy8I GTNNAC 1 cut(s) 404
Hpy99I CGWCG 1 cut(s) 243
HpyAV CCTTC 1 cut(s) 405
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 1 cut(s) 220
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 3 cut(s) 66, 258, 360
HpySE526I ACGT 1 cut(s) 397
Hsp92II CATG 3 cut(s) 247, 316, 334
HspAI GCGC 1 cut(s) 178
Kzo9I GATC 1 cut(s) 99
LguI GCTCTTC 1 cut(s) 222
LmnI GCTCC 1 cut(s) 141
Lsp1109I GCAGC 1 cut(s) 204
MaeI CTAG 1 cut(s) 381
MaeII ACGT 1 cut(s) 397
MalI GATC 1 cut(s) 101
MboI GATC 1 cut(s) 99
MboII GAAGA 5 cut(s) 40, 109, 119, 236, 239
MflI RGATCY 1 cut(s) 99
MluCI AATT 1 cut(s) 339
MlyI GAGTC 1 cut(s) 272
MnlI CCTC 6 cut(s) 46, 48, 103, 136, 253, 408
MseI TTAA 1 cut(s) 421
MspR9I CCNGG 3 cut(s) 44, 308, 345
Mva1269I GAATGC 1 cut(s) 180
MvaI CCWGG 3 cut(s) 44, 308, 345
MwoI GCNNNNNNNGC 1 cut(s) 217
NdeII GATC 1 cut(s) 99
NlaIII CATG 3 cut(s) 247, 316, 334
NlaIV GGNNCC 3 cut(s) 137, 192, 378
NspI RCATGY 2 cut(s) 316, 334
PciSI GCTCTTC 1 cut(s) 222
PctI GAATGC 1 cut(s) 180
PflFI GACNNNGTC 1 cut(s) 399
PkrI GCNGC 2 cut(s) 219, 366
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 191
Psp6I CCWGG 3 cut(s) 42, 306, 343
PspGI CCWGG 3 cut(s) 42, 306, 343
PspN4I GGNNCC 3 cut(s) 137, 192, 378
PspPI GGNCC 2 cut(s) 191, 401
PspPPI RGGWCCY 1 cut(s) 191
PstI CTGCAG 1 cut(s) 222
PsuI RGATCY 1 cut(s) 99
PsyI GACNNNGTC 1 cut(s) 399
RsaI GTAC 2 cut(s) 59, 255
RsaNI GTAC 2 cut(s) 58, 254
SapI GCTCTTC 1 cut(s) 222
SaqAI TTAA 1 cut(s) 421
SatI GCNGC 2 cut(s) 218, 365
Sau3AI GATC 1 cut(s) 99
Sau96I GGNCC 2 cut(s) 191, 401
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 3 cut(s) 44, 308, 345
SfcI CTRYAG 1 cut(s) 218
SinI GGWCC 2 cut(s) 191, 401
Sse9I AATT 1 cut(s) 339
SsiI CCGC 2 cut(s) 139, 365
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 3 cut(s) 42, 306, 343
StyI CCWWGG 2 cut(s) 75, 385
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 400
TasI AATT 1 cut(s) 339
TauI GCSGC 1 cut(s) 367
Tru1I TTAA 1 cut(s) 421
Tru9I TTAA 1 cut(s) 421
TscAI CASTG 1 cut(s) 30
TseI GCWGC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 188
TspRI CASTG 1 cut(s) 30
Tth111I GACNNNGTC 1 cut(s) 399
VpaK11BI GGWCC 2 cut(s) 191, 401
XagI CCTNNNNNAGG 2 cut(s) 130, 384
XapI RAATTY 1 cut(s) 339
XceI RCATGY 2 cut(s) 316, 334
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.