Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003428

Physical Location & Seq

Genomic Coordinates Reverse (-)

14611136 .. 14617660

Gene Structure 6525 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1096200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2148 bp

ATGATAATAAGATGTGCCGGGTTTGGGCCCCCGAAATATATGACCGACTTAACTCCAATTTTAATTTGTACATCCGTTCGTTTCGATACAAACCCAAAAAAAACCTCTGGAAAATTATTACCGCGAAATATGGGCAGTCACGGACGCAGCAGCCGAAGAAAGGAGGCGCCGTCGTCTTCCTCCAAGAGTCGCCGCAGAAGAAGATCCGACGACTCCGATTCCGTGTCGTATACCGACTCCGATGATTCCGACAGCCACGACTCCTCCCCGGCGAGGAGCTCCAGGAAGCGCAAGGAACGAAGCAGTCGCAGAAAGAGCAGCCGTCGGAAGGACTCCGACGACGAGTCGTACGACAGTGATGAGAGCGAGGAACGTGACCGCAAGACGAAGAGGTCGTCGAAGAACGTTACGGAGGAGCAAATCGCTGAGTACTTGGCTAAGAAGGCACAGAAGAAGGCAGCTCGAGTAGCCAAGAAGATAAAATCGAAGACGGTTTCGGGTTACTCGAATGATTCGAATCCCTTTGGCGATTCCAATCTCAATGAGATGTTTGTATGGCGAAAGAAGATTGACCGTGATGTGTCACATGGTGTTCCGCTTGACGAGTTTTCAGTTAAGGCTGAGAGAAAAAGACAGAGAGAAAGAATGGCAGAGATTGAAAAGGTTAAGAAAAGAAGGGAAGAAAGGGCACTTGAGAAAGCACAACATGAGGAAGAAATGGCACTGCTAGCCAGAGAACGTGCTCGGGCTGAGTTCCAAGACTGGGAGAAAAAAGAAGAAGAGTTCCACTTCGATCAAAGCAAAGTCAGGTCAGAGATCAGATTGCGTGAAGGGCGTATTAAGCCAATTGATGTATTGTCTAAACATCTGAATGGCTCAGATGACTTTGAGATTGAGATAGACGAGCCGTATATGGTCTTCATGGGATTGACTGTAAAGGAAATGGAAGAGCTTCGAGATGACATCAAAATGCACCTTGATTTGGACAGGGCAACAGAAACACATGTGAAGTACTGGGAGGCACTTTTAGTGGTTTGTGATTGGGAGCTAGCTGAAGGTCGGAAAAAAGATGCAATAGATCGAGCTAAGGTTCGTGGAGAGGAACCTCCTGCTGAGTTGCTTGCGGAACAAAGAGGCTTGCATACTAGCGTTGAAGCAGATGTCAAGAACCTCTTGGAAGGAAAGACTTTCAGGGAATTAGAGGAATTACAGTCCAAAATTGAGTCAGAAATGCGTTCTGGAACAGCCAAGGTTGTTGAGTATTGGGAGGCTGTTCTCACACGCCTCCATATATTCAAGGCCAAGGCTTGTTTGAAAGAAATCCATGCTAAAATGTTGCACAAGCATTTGCAACGGCTTCAGAAACGGGAGGATGGTGAAAAGAACTTGGATAGGGTTCATGATTCACAACCTGAAGAGGAGGAGAGTGAGTCTGATGTCAAAGATGCTCAAGCATTTTCTCCAGAACCCATGGAGGAGGAGATTCATGTGGCTGAGGAAGAGGCCGGGTCATTTTCACCAGAGTTGTTGCATGGAGATGAAAATGAGGAAGTAATCGACCCTGAAGAGGACAGGGCTATACTGGAAAGGAAGCGTGTAGCTGTGTTAGAAGAACAACAGAGGCGGATTCAAGAAGCTATGACATCAAAGCCAGCTCCACCAGAAGATAATTTTGAGATGAAGGCCATGAAATTCATGGGAGAAATGGTTGAAGGAGATGCAGTTCTTGGCACGGGTTCTGAAGTGAACCTTGACTCACAGGTATACTGGTGGCATGACAAGTACCGACCTAGAAAGCCAAAATATTTCAACCGTGTTCACACCGGATATGAGTGGAACAAGTACAATCAGACTCACTATGATCATGATAACCCTCCTCCGAAGATTGTGCAAGGATACAAGTTCAACATCTTCTATCCGGACCTTGTTGACAAGACGAAGGCCCCACATTATACGATAGAGAAGGATGGCAACAGCAGTGAGACATGCATTATAAGGTTCCATGCAGGGCCACCCTACGAGGACATTGCGTTCCGGATAGTAAACAAAGAATGGGAATACTCCCACAAAAAGGGGTTCAAATGCACATTTGAACGTGGAATTTTGCACGTATACTTCAACTTCAAACGACACCGCTATCGTAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CactinC_cactus Cactin_mid

Protein Analysis

716

Amino Acids

83.8

Weight (kDa)

6.54

Isoelectric Point (pI)

66.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cactin_mid	PF10312	245 - 442	1.7e-60	Conserved mid region of cactin
CactinC_cactus	PF09732	591 - 715	7.9e-64	Cactus-binding C-terminus of cactin protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003428)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03910 AT1G03910 AT2G36815 AT2G36815
fragaria_vesca	FvH4_6g17312 FvH4_6g17312 FvH4_6g17312 FvH4_6g17312 FvH4_6g17312
malus_domestica	MD12G1102100.v1.1 MD14G1096200.v1.1
prunus_persica	Prupe.7G122300_v2.0.a1
pyrus_communis	pycom14g09420
rosa_chinensis	RchiOBHm_Chr3g0471451 RchiOBHm_Chr3g0471601
rosa_laevigata	RLG00000024153 RLG00000024161 RLG00000024177
rosa_multiflora	Rmu_sc0006582.1_g000009 Rmu_sc0008048.1_g000005 Rmu_sc0037090.1_g000001
rosa_roxburghii	Rroxscaffold_6G00409750 Rroxscaffold_6G00409940
rosa_rugosa	Rorug03G0117800 Rorug03G0118500
rosa_samantha	Rh3AG167700 Rh3AG169100 Rh3AG169200 Rh3BG193200 Rh3BG194800 Rh3CG183600 Rh3CG185500 Rh3DG180000 Rh3DG182300
rosa_wichuraiana	Rw3G015800 Rw3G015900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1994
AasI	GACNNNNNNGTC	1 cut(s)	391
AccB1I	GGYRCC	1 cut(s)	166
AccI	GTMKAC	3 cut(s)	230, 1764, 2112
AccII	CGCG	1 cut(s)	124
AccIII	TCCGGA	2 cut(s)	1918, 2034
AciI	CCGC	7 cut(s)	122, 193, 379, 596, 1124, 1624, 2134
AclI	AACGTT	1 cut(s)	405
AclWI	GGATC	1 cut(s)	198
AcsI	RAATTY	2 cut(s)	1691, 2100
AcuI	CTGAAG	5 cut(s)	1074, 1343, 1434, 1584, 1761
AcyI	GRCGYC	1 cut(s)	167
AdeI	CACNNNGTG	1 cut(s)	590
AfaI	GTAC	6 cut(s)	70, 350, 431, 1013, 1784, 1844
AfiI	CCNNNNNNNGG	8 cut(s)	24, 160, 273, 328, 763, 982, 1567, 2071
AflIII	ACRYGT	1 cut(s)	1003
AhdI	GACNNNNNGTC	1 cut(s)	343
AjnI	CCWGG	1 cut(s)	281
AloI	GAACNNNNNNTCC	4 cut(s)	1707, 1739, 2015, 2047
AluBI	AGCT	9 cut(s)	279, 461, 952, 1048, 1052, 1085, 1601, 1637, 1655
AluI	AGCT	9 cut(s)	279, 461, 952, 1048, 1052, 1085, 1601, 1637, 1655
Alw21I	GWGCWC	2 cut(s)	281, 745
Alw26I	GTCTC	1 cut(s)	1976
AlwI	GGATC	1 cut(s)	198
Ama87I	CYCGRG	2 cut(s)	462, 744
Aor13HI	TCCGGA	2 cut(s)	1918, 2034
AoxI	GGCC	6 cut(s)	26, 1299, 1503, 1683, 1941, 2009
ApaI	GGGCCC	1 cut(s)	30
ApeKI	GCWGC	4 cut(s)	147, 150, 318, 458
ApoI	RAATTY	2 cut(s)	1691, 2100
ArsI	GACNNNNNNTTYG	2 cut(s)	380, 412
Asp700I	GAANNNNTTC	1 cut(s)	951
AspLEI	GCGC	2 cut(s)	169, 291
AspS9I	GGNCC	5 cut(s)	26, 27, 1921, 1942, 2009
AsuC2I	CCSGG	3 cut(s)	19, 269, 1507
AsuHPI	GGTGA	2 cut(s)	1388, 1509
AsuII	TTCGAA	1 cut(s)	515
AsuNHI	GCTAGC	2 cut(s)	727, 1048
AvaI	CYCGRG	2 cut(s)	462, 744
AvaII	GGWCC	1 cut(s)	1921
BaeGI	GKGCMC	2 cut(s)	30, 691
BanI	GGYRCC	1 cut(s)	166
BanII	GRGCYC	2 cut(s)	30, 281
BarI	GAAGNNNNNNTAC	2 cut(s)	902, 934
BbsI	GAAGAC	3 cut(s)	168, 494, 910
Bbv12I	GWGCWC	2 cut(s)	281, 745
BbvCI	CCTCAGC	1 cut(s)	1494
BbvI	GCAGC	4 cut(s)	159, 162, 330, 470
BccI	CCATC	2 cut(s)	1367, 1961
BceAI	ACGGC	4 cut(s)	154, 306, 892, 1370
BcgI	CGANNNNNNTGC	4 cut(s)	1870, 1904, 2009, 2043
BciT130I	CCWGG	1 cut(s)	283
BciVI	GTATCC	1 cut(s)	1889
BclI	TGATCA	1 cut(s)	1861
BcnI	CCSGG	3 cut(s)	19, 269, 1507
BcoDI	GTCTC	1 cut(s)	1976
BfaI	CTAG	4 cut(s)	728, 1049, 1146, 1791
BfoI	RGCGCY	1 cut(s)	170
BfuI	GTATCC	1 cut(s)	1889
BisI	GCNGC	5 cut(s)	148, 151, 193, 319, 459
BlsI	GCNGC	5 cut(s)	149, 152, 194, 320, 460
BmcAI	AGTACT	2 cut(s)	431, 1013
Bme1390I	CCNGG	4 cut(s)	19, 269, 283, 1507
Bme18I	GGWCC	1 cut(s)	1921
BmeRI	GACNNNNNGTC	1 cut(s)	343
BmeT110I	CYCGRG	2 cut(s)	462, 744
BmgT120I	GGNCC	5 cut(s)	26, 27, 1921, 1942, 2009
BmiI	GGNNCC	6 cut(s)	28, 29, 168, 1104, 1944, 2000
BmrFI	CCNGG	4 cut(s)	19, 269, 283, 1507
BmrI	ACTGGG	2 cut(s)	772, 1024
BmsI	GCATC	3 cut(s)	1060, 1435, 1708
BmtI	GCTAGC	2 cut(s)	731, 1052
BmuI	ACTGGG	2 cut(s)	772, 1024
BpiI	GAAGAC	3 cut(s)	168, 494, 910
BpmI	CTGGAG	2 cut(s)	265, 1446
Bpu10I	CCTNAGC	2 cut(s)	1086, 1494
Bpu14I	TTCGAA	1 cut(s)	515
BpuEI	CTTGAG	2 cut(s)	713, 1434
BpuMI	CCSGG	3 cut(s)	19, 269, 1507
BsaAI	YACGTR	1 cut(s)	2110
BsaBI	GATNNNNATC	2 cut(s)	516, 534
BsaHI	GRCGYC	1 cut(s)	167
BsaJI	CCNNGG	4 cut(s)	267, 1248, 1302, 1470
BsaWI	WCCGGW	3 cut(s)	1823, 1918, 2034
Bsc4I	CCNNNNNNNGG	8 cut(s)	24, 160, 273, 328, 763, 982, 1567, 2071
Bse1I	ACTGG	4 cut(s)	767, 1019, 1587, 1772
Bse3DI	GCAATG	1 cut(s)	2025
Bse8I	GATNNNNATC	2 cut(s)	516, 534
BseAI	TCCGGA	2 cut(s)	1918, 2034
BseBI	CCWGG	1 cut(s)	283
BseDI	CCNNGG	4 cut(s)	267, 1248, 1302, 1470
BseGI	GGATG	3 cut(s)	71, 1378, 1972
BseJI	GATNNNNATC	2 cut(s)	516, 534
BseLI	CCNNNNNNNGG	8 cut(s)	24, 160, 273, 328, 763, 982, 1567, 2071
BseMI	GCAATG	1 cut(s)	2025
BseMII	CTCAG	6 cut(s)	417, 612, 741, 891, 1104, 1485
BseNI	ACTGG	4 cut(s)	767, 1019, 1587, 1772
BseRI	GAGGAG	8 cut(s)	253, 289, 428, 1433, 1436, 1490, 1493, 1866
BseSI	GKGCMC	2 cut(s)	30, 691
BseXI	GCAGC	4 cut(s)	159, 162, 330, 470
Bsh1236I	CGCG	1 cut(s)	124
BshFI	GGCC	6 cut(s)	28, 1301, 1505, 1685, 1943, 2011
BshNI	GGYRCC	1 cut(s)	166
BsiHKAI	GWGCWC	2 cut(s)	281, 745
BsiHKCI	CYCGRG	2 cut(s)	462, 744
BsiSI	CCGG	6 cut(s)	18, 269, 1506, 1824, 1919, 2035
BsiWI	CGTACG	1 cut(s)	348
BslI	CCNNNNNNNGG	8 cut(s)	24, 160, 273, 328, 763, 982, 1567, 2071
BsmAI	GTCTC	1 cut(s)	1976
BsnI	GGCC	6 cut(s)	28, 1301, 1505, 1685, 1943, 2011
BsoBI	CYCGRG	2 cut(s)	462, 744
Bsp119I	TTCGAA	1 cut(s)	515
Bsp120I	GGGCCC	1 cut(s)	26
Bsp1286I	GDGCHC	4 cut(s)	30, 281, 691, 745
Bsp13I	TCCGGA	2 cut(s)	1918, 2034
Bsp1407I	TGTACA	1 cut(s)	68
Bsp143I	GATC	5 cut(s)	203, 793, 816, 1078, 1861
Bsp19I	CCATGG	1 cut(s)	1470
BspACI	CCGC	7 cut(s)	122, 193, 379, 596, 1124, 1624, 2134
BspANI	GGCC	6 cut(s)	28, 1301, 1505, 1685, 1943, 2011
BspCNI	CTCAG	6 cut(s)	418, 613, 742, 890, 1105, 1486
BspEI	TCCGGA	2 cut(s)	1918, 2034
BspFNI	CGCG	1 cut(s)	124
BspHI	TCATGA	2 cut(s)	1399, 1864
BspLI	GGNNCC	6 cut(s)	28, 29, 168, 1104, 1944, 2000
BspOI	GCTAGC	2 cut(s)	731, 1052
BspPI	GGATC	1 cut(s)	198
BspQI	GCTCTTC	1 cut(s)	942
BspT104I	TTCGAA	1 cut(s)	515
BspT107I	GGYRCC	1 cut(s)	166
BsrDI	GCAATG	1 cut(s)	2025
BsrGI	TGTACA	1 cut(s)	68
BsrI	ACTGG	4 cut(s)	767, 1019, 1587, 1772
BssECI	CCNNGG	4 cut(s)	267, 1248, 1302, 1470
BssMI	GATC	5 cut(s)	203, 793, 816, 1078, 1861
BssNAI	GTATAC	3 cut(s)	231, 1765, 2113
BssNI	GRCGYC	1 cut(s)	167
BssT1I	CCWWGG	3 cut(s)	1248, 1302, 1470
Bst1107I	GTATAC	3 cut(s)	231, 1765, 2113
Bst2UI	CCWGG	1 cut(s)	283
Bst4CI	ACNGT	6 cut(s)	356, 493, 575, 934, 1212, 1814
Bst6I	CTCTTC	6 cut(s)	383, 774, 942, 1410, 1494, 1560
BstACI	GRCGYC	1 cut(s)	167
BstAUI	TGTACA	1 cut(s)	68
BstBAI	YACGTR	1 cut(s)	2110
BstBI	TTCGAA	1 cut(s)	515
BstC8I	GCNNGC	5 cut(s)	729, 1050, 1122, 1139, 1653
BstDEI	CTNAG	8 cut(s)	426, 438, 621, 750, 877, 1086, 1113, 1494
BstDSI	CCRYGG	1 cut(s)	1470
BstF5I	GGATG	3 cut(s)	71, 1378, 1972
BstFNI	CGCG	1 cut(s)	124
BstH2I	RGCGCY	1 cut(s)	170
BstHHI	GCGC	2 cut(s)	169, 291
BstKTI	GATC	5 cut(s)	206, 796, 819, 1081, 1864
BstMAI	GTCTC	1 cut(s)	1976
BstMBI	GATC	5 cut(s)	203, 793, 816, 1078, 1861
BstMWI	GCNNNNNNNGC	6 cut(s)	315, 443, 467, 728, 832, 841
BstNI	CCWGG	1 cut(s)	283
BstNSI	RCATGY	2 cut(s)	1007, 1989
BstSCI	CCNGG	4 cut(s)	17, 267, 281, 1505
BstSLI	GKGCMC	2 cut(s)	30, 691
BstUI	CGCG	1 cut(s)	124
BstV1I	GCAGC	4 cut(s)	159, 162, 330, 470
BstV2I	GAAGAC	3 cut(s)	168, 494, 910
BstX2I	RGATCY	1 cut(s)	203
BstYI	RGATCY	1 cut(s)	203
BstZ17I	GTATAC	3 cut(s)	231, 1765, 2113
BsuI	GTATCC	1 cut(s)	1889
BsuRI	GGCC	6 cut(s)	28, 1301, 1505, 1685, 1943, 2011
BtgI	CCRYGG	1 cut(s)	1470
BtsCI	GGATG	3 cut(s)	71, 1378, 1972
BtsI	GCAGTG	2 cut(s)	722, 1984
BtsIMutI	CAGTG	3 cut(s)	361, 722, 1984
Cac8I	GCNNGC	5 cut(s)	729, 1050, 1122, 1139, 1653
CciI	TCATGA	2 cut(s)	1399, 1864
CfoI	GCGC	2 cut(s)	169, 291
Cfr13I	GGNCC	5 cut(s)	26, 27, 1921, 1942, 2009
CseI	GACGC	1 cut(s)	153
Csp6I	GTAC	6 cut(s)	69, 349, 430, 1012, 1783, 1843
CviQI	GTAC	6 cut(s)	69, 349, 430, 1012, 1783, 1843
DdeI	CTNAG	8 cut(s)	426, 438, 621, 750, 877, 1086, 1113, 1494
DinI	GGCGCC	1 cut(s)	168
DpnI	GATC	5 cut(s)	205, 795, 818, 1080, 1863
DpnII	GATC	5 cut(s)	203, 793, 816, 1078, 1861
DraIII	CACNNNGTG	1 cut(s)	590
DrdI	GACNNNNNNGTC	1 cut(s)	391
DriI	GACNNNNNGTC	1 cut(s)	343
DseDI	GACNNNNNNGTC	1 cut(s)	391
Eam1104I	CTCTTC	6 cut(s)	383, 774, 942, 1410, 1494, 1560
Eam1105I	GACNNNNNGTC	1 cut(s)	343
EarI	CTCTTC	6 cut(s)	383, 774, 942, 1410, 1494, 1560
EciI	GGCGGA	1 cut(s)	1639
Ecl136II	GAGCTC	1 cut(s)	279
Eco130I	CCWWGG	3 cut(s)	1248, 1302, 1470
Eco24I	GRGCYC	2 cut(s)	30, 281
Eco47I	GGWCC	1 cut(s)	1921
Eco53kI	GAGCTC	1 cut(s)	279
Eco57I	CTGAAG	5 cut(s)	1074, 1343, 1434, 1584, 1761
Eco88I	CYCGRG	2 cut(s)	462, 744
EcoICRI	GAGCTC	1 cut(s)	279
EcoO109I	RGGNCCY	2 cut(s)	27, 1942
EcoRII	CCWGG	1 cut(s)	281
EcoT14I	CCWWGG	3 cut(s)	1248, 1302, 1470
EcoT22I	ATGCAT	1 cut(s)	1991
EcoT38I	GRGCYC	2 cut(s)	30, 281
EgeI	GGCGCC	1 cut(s)	168
EheI	GGCGCC	1 cut(s)	168
ErhI	CCWWGG	3 cut(s)	1248, 1302, 1470
FalI	AAGNNNNNCTT	4 cut(s)	1157, 1189, 1734, 1766
FbaI	TGATCA	1 cut(s)	1861
FblI	GTMKAC	3 cut(s)	230, 1764, 2112
Fnu4HI	GCNGC	5 cut(s)	148, 151, 193, 319, 459
FokI	GGATG	3 cut(s)	58, 1385, 1979
FriOI	GRGCYC	2 cut(s)	30, 281
Fsp4HI	GCNGC	5 cut(s)	148, 151, 193, 319, 459
FspBI	CTAG	4 cut(s)	728, 1049, 1146, 1791
GlaI	GCGC	2 cut(s)	168, 290
GluI	GCNGC	5 cut(s)	148, 151, 193, 319, 459
GsuI	CTGGAG	2 cut(s)	265, 1446
HaeII	RGCGCY	1 cut(s)	170
HaeIII	GGCC	6 cut(s)	28, 1301, 1505, 1685, 1943, 2011
HapII	CCGG	6 cut(s)	18, 269, 1506, 1824, 1919, 2035
HgaI	GACGC	1 cut(s)	153
HhaI	GCGC	2 cut(s)	169, 291
Hin1I	GRCGYC	1 cut(s)	167
Hin6I	GCGC	2 cut(s)	167, 289
HinP1I	GCGC	2 cut(s)	167, 289
HincII	GTYRAC	1 cut(s)	1930
HindII	GTYRAC	1 cut(s)	1930
HpaII	CCGG	6 cut(s)	18, 269, 1506, 1824, 1919, 2035
HphI	GGTGA	2 cut(s)	1388, 1509
Hpy166II	GTNNAC	7 cut(s)	231, 1747, 1765, 1819, 1930, 2044, 2113
Hpy8I	GTNNAC	7 cut(s)	231, 1747, 1765, 1819, 1930, 2044, 2113
Hpy99I	CGWCG	6 cut(s)	175, 212, 327, 341, 344, 400
HpyCH4III	ACNGT	6 cut(s)	356, 493, 575, 934, 1212, 1814
HpyCH4IV	ACGT	5 cut(s)	373, 405, 739, 2095, 2109
HpyF10VI	GCNNNNNNNGC	6 cut(s)	315, 443, 467, 728, 832, 841
HpyF3I	CTNAG	8 cut(s)	426, 438, 621, 750, 877, 1086, 1113, 1494
HpySE526I	ACGT	5 cut(s)	373, 405, 739, 2095, 2109
Hsp92I	GRCGYC	1 cut(s)	167
HspAI	GCGC	2 cut(s)	167, 289
KasI	GGCGCC	1 cut(s)	166
Kpn2I	TCCGGA	2 cut(s)	1918, 2034
Ksp22I	TGATCA	1 cut(s)	1861
Kzo9I	GATC	5 cut(s)	203, 793, 816, 1078, 1861
LguI	GCTCTTC	1 cut(s)	942
LmnI	GCTCC	5 cut(s)	276, 284, 415, 1045, 1660
Lsp1109I	GCAGC	4 cut(s)	159, 162, 330, 470
LweI	GCATC	3 cut(s)	1060, 1435, 1708
MaeI	CTAG	4 cut(s)	728, 1049, 1146, 1791
MaeII	ACGT	5 cut(s)	373, 405, 739, 2095, 2109
MaeIII	GTNAC	5 cut(s)	137, 374, 406, 500, 582
MalI	GATC	5 cut(s)	205, 795, 818, 1080, 1863
MboI	GATC	5 cut(s)	203, 793, 816, 1078, 1861
MfeI	CAATTG	1 cut(s)	846
MflI	RGATCY	1 cut(s)	203
MhlI	GDGCHC	4 cut(s)	30, 281, 691, 745
Mly113I	GGCGCC	1 cut(s)	167
MmeI	TCCRAC	5 cut(s)	231, 273, 305, 360, 1040
Mph1103I	ATGCAT	1 cut(s)	1991
MroI	TCCGGA	2 cut(s)	1918, 2034
MroXI	GAANNNNTTC	1 cut(s)	951
MseI	TTAA	5 cut(s)	50, 62, 615, 666, 840
MslI	CAYNNNNRTG	3 cut(s)	870, 968, 1536
MspI	CCGG	6 cut(s)	18, 269, 1506, 1824, 1919, 2035
MspR9I	CCNGG	4 cut(s)	19, 269, 283, 1507
MunI	CAATTG	1 cut(s)	846
MvaI	CCWGG	1 cut(s)	283
MvnI	CGCG	1 cut(s)	124
MwoI	GCNNNNNNNGC	6 cut(s)	315, 443, 467, 728, 832, 841
NarI	GGCGCC	1 cut(s)	167
NciI	CCSGG	3 cut(s)	19, 269, 1507
NcoI	CCATGG	1 cut(s)	1470
NdeII	GATC	5 cut(s)	203, 793, 816, 1078, 1861
NheI	GCTAGC	2 cut(s)	727, 1048
NlaIV	GGNNCC	6 cut(s)	28, 29, 168, 1104, 1944, 2000
NmuCI	GTSAC	3 cut(s)	137, 374, 582
NsiI	ATGCAT	1 cut(s)	1991
NspI	RCATGY	2 cut(s)	1007, 1989
NspV	TTCGAA	1 cut(s)	515
PaeR7I	CTCGAG	1 cut(s)	462
PagI	TCATGA	2 cut(s)	1399, 1864
PciI	ACATGT	1 cut(s)	1003
PciSI	GCTCTTC	1 cut(s)	942
PcsI	WCGNNNNNNNCGW	5 cut(s)	151, 348, 392, 503, 512
PdmI	GAANNNNTTC	1 cut(s)	951
PfeI	GAWTC	8 cut(s)	218, 245, 512, 517, 530, 1403, 1483, 1627
Pfl23II	CGTACG	1 cut(s)	348
PfoI	TCCNGGA	1 cut(s)	281
PkrI	GCNGC	5 cut(s)	149, 152, 194, 320, 460
PluTI	GGCGCC	1 cut(s)	170
Ppu21I	YACGTR	1 cut(s)	2110
PscI	ACATGT	1 cut(s)	1003
PsiI	TTATAA	1 cut(s)	1994
Psp124BI	GAGCTC	1 cut(s)	281
Psp1406I	AACGTT	1 cut(s)	405
Psp6I	CCWGG	1 cut(s)	281
PspGI	CCWGG	1 cut(s)	281
PspLI	CGTACG	1 cut(s)	348
PspN4I	GGNNCC	6 cut(s)	28, 29, 168, 1104, 1944, 2000
PspOMI	GGGCCC	1 cut(s)	26
PspPI	GGNCC	5 cut(s)	26, 27, 1921, 1942, 2009
PspXI	VCTCGAGB	1 cut(s)	462
PsuI	RGATCY	1 cut(s)	203
RsaI	GTAC	6 cut(s)	70, 350, 431, 1013, 1784, 1844
RsaNI	GTAC	6 cut(s)	69, 349, 430, 1012, 1783, 1843
RseI	CAYNNNNRTG	3 cut(s)	870, 968, 1536
SacI	GAGCTC	1 cut(s)	281
SapI	GCTCTTC	1 cut(s)	942
SaqAI	TTAA	5 cut(s)	50, 62, 615, 666, 840
SatI	GCNGC	5 cut(s)	148, 151, 193, 319, 459
Sau3AI	GATC	5 cut(s)	203, 793, 816, 1078, 1861
Sau96I	GGNCC	5 cut(s)	26, 27, 1921, 1942, 2009
ScaI	AGTACT	2 cut(s)	431, 1013
ScrFI	CCNGG	4 cut(s)	19, 269, 283, 1507
SduI	GDGCHC	4 cut(s)	30, 281, 691, 745
SfaNI	GCATC	3 cut(s)	1060, 1435, 1708
SfoI	GGCGCC	1 cut(s)	168
Sfr274I	CTCGAG	1 cut(s)	462
SfuI	TTCGAA	1 cut(s)	515
SinI	GGWCC	1 cut(s)	1921
SlaI	CTCGAG	1 cut(s)	462
SmiMI	CAYNNNNRTG	3 cut(s)	870, 968, 1536
SmlI	CTYRAG	3 cut(s)	462, 692, 1449
SmoI	CTYRAG	3 cut(s)	462, 692, 1449
SsiI	CCGC	7 cut(s)	122, 193, 379, 596, 1124, 1624, 2134
SspDI	GGCGCC	1 cut(s)	166
SspI	AATATT	1 cut(s)	1805
SspMI	CTAG	4 cut(s)	728, 1049, 1146, 1791
SstI	GAGCTC	1 cut(s)	281
StyD4I	CCNGG	4 cut(s)	17, 267, 281, 1505
StyI	CCWWGG	3 cut(s)	1248, 1302, 1470
TaaI	ACNGT	6 cut(s)	356, 493, 575, 934, 1212, 1814
TaiI	ACGT	5 cut(s)	376, 408, 742, 2098, 2112
TaqII	GACCGA	1 cut(s)	59
TatI	WGTACW	4 cut(s)	68, 429, 1011, 1842
TauI	GCSGC	1 cut(s)	195
TfiI	GAWTC	8 cut(s)	218, 245, 512, 517, 530, 1403, 1483, 1627
Tru1I	TTAA	5 cut(s)	50, 62, 615, 666, 840
Tru9I	TTAA	5 cut(s)	50, 62, 615, 666, 840
TscAI	CASTG	3 cut(s)	361, 729, 1984
TseFI	GTSAC	3 cut(s)	137, 374, 582
TseI	GCWGC	4 cut(s)	147, 150, 318, 458
Tsp45I	GTSAC	3 cut(s)	137, 374, 582
TspDTI	ATGAA	7 cut(s)	910, 1388, 1475, 1554, 1684, 1694, 1703
TspGWI	ACGGA	4 cut(s)	64, 156, 211, 425
TspRI	CASTG	3 cut(s)	361, 729, 1984
VpaK11BI	GGWCC	1 cut(s)	1921
XapI	RAATTY	2 cut(s)	1691, 2100
XceI	RCATGY	2 cut(s)	1007, 1989
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1693
XhoI	CTCGAG	1 cut(s)	462
XmiI	GTMKAC	3 cut(s)	230, 1764, 2112
XmnI	GAANNNNTTC	1 cut(s)	951
XspI	CTAG	4 cut(s)	728, 1049, 1146, 1791
ZrmI	AGTACT	2 cut(s)	431, 1013
Zsp2I	ATGCAT	1 cut(s)	1991

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.