Rh3DG180000

Cactus-binding C-terminus of cactin protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0003428
Physical Location & Seq
Forward (+)
16051844 .. 16053242
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG180000.1

Sequence Viewer

Length: 648 bp
ATGACCTTTCTTTATGTACAAGTGCTTCCAACTTCCAAGATTATTCACCTTCAATTTCAACATTGCAACTCTACTGTGGACTTGATCAAGAGAGTTCTGCGCACAAGTAAATTGTCACTTTTGGTGGTTTGCAATTGGGAGCTATCAGAAGCTCGGAAAAAGGATGCTTTGGATCAAGCTAGGGTTCGTTGGGAGGAACCTCCTGCCAAGTTACTTGCTGAACAAAGAGGCTTATATTCTAGCATTGAAGCAGACTTCAAGTATCTCTTGGAAGAGAAGAATTATAGTCAGTTGGAGGCATTACATGCTGAAATTGAGTCACAGATGCGTTCTGGTACAGCCAAGGTTGTTGCGTATTGGGAGGCTGTTCTTAGACGCCTCCATGTATTCAAGGCCAAGGCCTGTTTGAAAGAAATCCATGCTAAAATGTTGCACAAGCAGGAGAGTGAGCCTGATGCTAATGGTGCTGAAACATATACATCAGAAACCGTAGAGGAGATCCACATGGCTGAAGAGGCCGGTTCATTTTCACAAGAACTATTACATGGAGATGAAAATGAGGACGTGAATGACCCTGAAGAGAATAGGGCTATACTGGAACACAAGCGTACAGCTTTATTAGAACAACAACAACCACGAATTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CactinC_cactus Cactin_mid

Protein Analysis

215

Amino Acids

24.82

Weight (kDa)

5.53

Isoelectric Point (pI)

58.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cactin_mid PF10312 39 - 140 7.9e-21 Conserved mid region of cactin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 101
AclWI GGATC 2 cut(s) 180, 493
AcsI RAATTY 1 cut(s) 639
AcuI CTGAAG 2 cut(s) 531, 597
AcyI GRCGYC 1 cut(s) 376
AfaI GTAC 3 cut(s) 18, 337, 610
AgsI TTSAA 7 cut(s) 53, 59, 248, 259, 391, 409, 644
AjiI CACGTC 1 cut(s) 565
AluBI AGCT 4 cut(s) 142, 152, 179, 614
AluI AGCT 4 cut(s) 142, 152, 179, 614
AlwI GGATC 2 cut(s) 180, 493
AoxI GGCC 3 cut(s) 393, 399, 516
ApoI RAATTY 1 cut(s) 639
AspLEI GCGC 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 38
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BclI TGATCA 1 cut(s) 84
BfaI CTAG 2 cut(s) 180, 240
BmgBI CACGTC 1 cut(s) 565
BmiI GGNNCC 1 cut(s) 198
BmsI GCATC 3 cut(s) 154, 315, 445
BsaHI GRCGYC 1 cut(s) 376
BsaJI CCNNGG 2 cut(s) 342, 396
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 1 cut(s) 600
Bse3DI GCAATG 1 cut(s) 61
BseDI CCNNGG 2 cut(s) 342, 396
BseGI GGATG 1 cut(s) 169
BseMI GCAATG 1 cut(s) 61
BseNI ACTGG 1 cut(s) 600
BseRI GAGGAG 1 cut(s) 509
BshFI GGCC 3 cut(s) 395, 401, 518
BsiSI CCGG 1 cut(s) 519
BsnI GGCC 3 cut(s) 395, 401, 518
Bsp1407I TGTACA 1 cut(s) 16
Bsp143I GATC 3 cut(s) 84, 172, 498
BspANI GGCC 3 cut(s) 395, 401, 518
BspLI GGNNCC 1 cut(s) 198
BspPI GGATC 2 cut(s) 180, 493
BsrDI GCAATG 1 cut(s) 61
BsrFI RCCGGY 1 cut(s) 518
BsrGI TGTACA 1 cut(s) 16
BsrI ACTGG 1 cut(s) 600
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 2 cut(s) 342, 396
BssMI GATC 3 cut(s) 84, 172, 498
BssNI GRCGYC 1 cut(s) 376
BssT1I CCWWGG 2 cut(s) 342, 396
Bst4CI ACNGT 2 cut(s) 76, 490
Bst6I CTCTTC 3 cut(s) 267, 507, 573
BstACI GRCGYC 1 cut(s) 376
BstAPI GCANNNNNTGC 1 cut(s) 305
BstAUI TGTACA 1 cut(s) 16
BstDEI CTNAG 1 cut(s) 371
BstF5I GGATG 1 cut(s) 169
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 3 cut(s) 87, 175, 501
BstMBI GATC 3 cut(s) 84, 172, 498
BstMWI GCNNNNNNNGC 3 cut(s) 305, 464, 515
BstNSI RCATGY 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 498
BstYI RGATCY 1 cut(s) 498
BsuRI GGCC 3 cut(s) 395, 401, 518
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 1 cut(s) 169
CfoI GCGC 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 518
CseI GACGC 1 cut(s) 384
Csp6I GTAC 3 cut(s) 17, 336, 609
CviAII CATG 5 cut(s) 305, 383, 419, 505, 545
CviQI GTAC 3 cut(s) 17, 336, 609
DdeI CTNAG 1 cut(s) 371
DpnI GATC 3 cut(s) 86, 174, 500
DpnII GATC 3 cut(s) 84, 172, 498
Eam1104I CTCTTC 3 cut(s) 267, 507, 573
EarI CTCTTC 3 cut(s) 267, 507, 573
Eco130I CCWWGG 2 cut(s) 342, 396
Eco147I AGGCCT 1 cut(s) 401
Eco57I CTGAAG 2 cut(s) 531, 597
EcoRI GAATTC 1 cut(s) 639
EcoT14I CCWWGG 2 cut(s) 342, 396
ErhI CCWWGG 2 cut(s) 342, 396
FaeI CATG 5 cut(s) 308, 386, 422, 508, 548
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FatI CATG 5 cut(s) 304, 382, 418, 504, 544
FbaI TGATCA 1 cut(s) 84
FokI GGATG 1 cut(s) 176
FspBI CTAG 2 cut(s) 180, 240
FspI TGCGCA 1 cut(s) 101
GlaI GCGC 1 cut(s) 101
HaeIII GGCC 3 cut(s) 395, 401, 518
HapII CCGG 1 cut(s) 519
HgaI GACGC 1 cut(s) 384
HhaI GCGC 1 cut(s) 102
Hin1I GRCGYC 1 cut(s) 376
Hin1II CATG 5 cut(s) 308, 386, 422, 508, 548
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HinfI GANTC 1 cut(s) 317
HpaII CCGG 1 cut(s) 519
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 3 cut(s) 148, 156, 484
Hpy188III TCNNGA 1 cut(s) 88
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 76, 490
HpyCH4IV ACGT 1 cut(s) 564
HpyCH4V TGCA 3 cut(s) 66, 132, 433
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 464, 515
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 1 cut(s) 564
Hsp92I GRCGYC 1 cut(s) 376
Hsp92II CATG 5 cut(s) 308, 386, 422, 508, 548
HspAI GCGC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 84
Kzo9I GATC 3 cut(s) 84, 172, 498
LmnI GCTCC 1 cut(s) 139
LpnPI CCDG 8 cut(s) 216, 318, 415, 425, 465, 532, 581, 588
LweI GCATC 3 cut(s) 154, 315, 445
MaeI CTAG 2 cut(s) 180, 240
MaeII ACGT 1 cut(s) 564
MaeIII GTNAC 3 cut(s) 114, 210, 318
MalI GATC 3 cut(s) 86, 174, 500
MboI GATC 3 cut(s) 84, 172, 498
MboII GAAGA 4 cut(s) 284, 289, 524, 590
MfeI CAATTG 1 cut(s) 133
MflI RGATCY 1 cut(s) 498
MluCI AATT 6 cut(s) 53, 110, 133, 280, 312, 639
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 2 cut(s) 53, 273
MnlI CCTC 9 cut(s) 187, 210, 221, 289, 355, 389, 487, 508, 553
MslI CAYNNNNRTG 1 cut(s) 549
MspI CCGG 1 cut(s) 519
MunI CAATTG 1 cut(s) 133
MwoI GCNNNNNNNGC 3 cut(s) 305, 464, 515
NdeII GATC 3 cut(s) 84, 172, 498
NlaIII CATG 5 cut(s) 308, 386, 422, 508, 548
NlaIV GGNNCC 1 cut(s) 198
NmuCI GTSAC 2 cut(s) 114, 318
NsbI TGCGCA 1 cut(s) 101
NspI RCATGY 1 cut(s) 308
PceI AGGCCT 1 cut(s) 401
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
PspN4I GGNNCC 1 cut(s) 198
PsuI RGATCY 1 cut(s) 498
RsaI GTAC 3 cut(s) 18, 337, 610
RsaNI GTAC 3 cut(s) 17, 336, 609
RseI CAYNNNNRTG 1 cut(s) 549
Sau3AI GATC 3 cut(s) 84, 172, 498
SchI GAGTC 1 cut(s) 326
SetI ASST 9 cut(s) 8, 51, 144, 154, 181, 202, 348, 567, 616
SfaNI GCATC 3 cut(s) 154, 315, 445
SmiMI CAYNNNNRTG 1 cut(s) 549
Sse9I AATT 6 cut(s) 53, 110, 133, 280, 312, 639
SseBI AGGCCT 1 cut(s) 401
SspMI CTAG 2 cut(s) 180, 240
StuI AGGCCT 1 cut(s) 401
StyI CCWWGG 2 cut(s) 342, 396
TaaI ACNGT 2 cut(s) 76, 490
TaiI ACGT 1 cut(s) 567
TasI AATT 6 cut(s) 53, 110, 133, 280, 312, 639
TatI WGTACW 1 cut(s) 16
TseFI GTSAC 2 cut(s) 114, 318
Tsp45I GTSAC 2 cut(s) 114, 318
TspDTI ATGAA 2 cut(s) 513, 567
XapI RAATTY 1 cut(s) 639
XceI RCATGY 1 cut(s) 308
XspI CTAG 2 cut(s) 180, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.