Rw3G015900

Cactus-binding C-terminus of cactin protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003428
Physical Location & Seq
Reverse (-)
16471880 .. 16472875
996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015900.1

Sequence Viewer

Length: 723 bp
ATGGTGCCCGAAAATGAAGTATTTGAAAACCAGGCCGGTTTGATGATGAAGCATTTCTTGGTATGTGCCATTTGGCAGTCACTTTTGGTGGTTTGCAATTGGGAGCTATCAGAAGCTCGGAAAAAGGATGCTTTGGATCAAGCTAGGGTTCTTTGGGAGGAACCTCCTGCCAAGTTACTTGCTGAACAAAGAGGCTTATATTCTAGCATTGAAGCAGACTTCAAGTATCTCTTGGAAGAGAAGAATGATAGTCAGTTGGAGGCATTACATGCTGAAATTGAGTCACAGATGCGTTCTGGTACAGCCAAGGTTATTGCGTATTGGGAGGCTGTTCTTAGACGCCTCCATGTATTCAAGGCCAAGGCCTGTTTGAAAGAAATCCATGCTAAAATGTTGCACAAGCGTCTGCAACACCTTGAGGGGCGACAGGATGGTGAAGAGAAATTGGATATGCCCGATGATTTACAACCTGAAGAGGAGAGTGAGCCTGATGCTAATGGTGCTGAAACATATACATCAGAACCCGTAGAGGAGATCCACATGGCTGAAGAGGTCGGTTCATTTTCACAAGAACTATTACATGGAGATGAAAATGAGGACGTGAATGACCCAGAAGAGAATAGGGCTATACTGGAACACAAGCGTACAGCTTTATTAGAACAACAACAACCACGAATTCAAGAAGCCATGGCATCAAAGCCAGCTCCACCACCAGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CactinC_cactus Cactin_mid

Protein Analysis

240

Amino Acids

27.52

Weight (kDa)

4.7

Isoelectric Point (pI)

70.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cactin_mid PF10312 25 - 128 5.5e-21 Conserved mid region of cactin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AclWI GGATC 2 cut(s) 144, 529
AcsI RAATTY 1 cut(s) 675
AcuI CTGAAG 2 cut(s) 492, 567
AcyI GRCGYC 1 cut(s) 340
AfaI GTAC 2 cut(s) 301, 646
AgsI TTSAA 6 cut(s) 26, 212, 223, 355, 373, 680
AjiI CACGTC 1 cut(s) 601
AjnI CCWGG 1 cut(s) 30
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AluBI AGCT 5 cut(s) 106, 116, 143, 650, 704
AluI AGCT 5 cut(s) 106, 116, 143, 650, 704
AlwI GGATC 2 cut(s) 144, 529
AoxI GGCC 3 cut(s) 33, 357, 363
ApoI RAATTY 1 cut(s) 675
Asp700I GAANNNNTTC 1 cut(s) 53
AsuHPI GGTGA 1 cut(s) 446
BaeGI GKGCMC 1 cut(s) 9
BanI GGYRCC 1 cut(s) 4
BccI CCATC 1 cut(s) 425
BciT130I CCWGG 1 cut(s) 32
BfaI CTAG 2 cut(s) 144, 204
Bme1390I CCNGG 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 601
BmiI GGNNCC 2 cut(s) 6, 162
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 4 cut(s) 118, 279, 481, 701
BpuEI CTTGAG 1 cut(s) 437
BsaHI GRCGYC 1 cut(s) 340
BsaJI CCNNGG 3 cut(s) 306, 360, 687
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 636
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 3 cut(s) 306, 360, 687
BseGI GGATG 2 cut(s) 133, 436
BseNI ACTGG 1 cut(s) 636
BseRI GAGGAG 2 cut(s) 491, 545
BseSI GKGCMC 1 cut(s) 9
BshFI GGCC 3 cut(s) 35, 359, 365
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 1 cut(s) 36
BsnI GGCC 3 cut(s) 35, 359, 365
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 2 cut(s) 136, 534
Bsp19I CCATGG 1 cut(s) 687
BspANI GGCC 3 cut(s) 35, 359, 365
BspLI GGNNCC 2 cut(s) 6, 162
BspPI GGATC 2 cut(s) 144, 529
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 1 cut(s) 636
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 3 cut(s) 306, 360, 687
BssMI GATC 2 cut(s) 136, 534
BssNI GRCGYC 1 cut(s) 340
BssT1I CCWWGG 3 cut(s) 306, 360, 687
Bst2UI CCWGG 1 cut(s) 32
Bst6I CTCTTC 5 cut(s) 231, 432, 468, 543, 609
BstACI GRCGYC 1 cut(s) 340
BstAPI GCANNNNNTGC 1 cut(s) 269
BstC8I GCNNGC 1 cut(s) 702
BstDEI CTNAG 1 cut(s) 335
BstDSI CCRYGG 1 cut(s) 687
BstF5I GGATG 2 cut(s) 133, 436
BstKTI GATC 2 cut(s) 139, 537
BstMBI GATC 2 cut(s) 136, 534
BstMWI GCNNNNNNNGC 2 cut(s) 269, 500
BstNI CCWGG 1 cut(s) 32
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 30
BstSLI GKGCMC 1 cut(s) 9
BstX2I RGATCY 1 cut(s) 534
BstYI RGATCY 1 cut(s) 534
BsuRI GGCC 3 cut(s) 35, 359, 365
BtgI CCRYGG 1 cut(s) 687
BtrI CACGTC 1 cut(s) 601
BtsCI GGATG 2 cut(s) 133, 436
Cac8I GCNNGC 1 cut(s) 702
Cfr10I RCCGGY 1 cut(s) 35
CseI GACGC 2 cut(s) 348, 392
Csp6I GTAC 2 cut(s) 300, 645
CviAII CATG 6 cut(s) 269, 347, 383, 541, 581, 688
CviQI GTAC 2 cut(s) 300, 645
DdeI CTNAG 1 cut(s) 335
DpnI GATC 2 cut(s) 138, 536
DpnII GATC 2 cut(s) 136, 534
Eam1104I CTCTTC 5 cut(s) 231, 432, 468, 543, 609
EarI CTCTTC 5 cut(s) 231, 432, 468, 543, 609
Eco130I CCWWGG 3 cut(s) 306, 360, 687
Eco147I AGGCCT 1 cut(s) 365
Eco57I CTGAAG 2 cut(s) 492, 567
EcoRI GAATTC 1 cut(s) 675
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 3 cut(s) 306, 360, 687
ErhI CCWWGG 3 cut(s) 306, 360, 687
FaeI CATG 6 cut(s) 272, 350, 386, 544, 584, 691
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 215, 247
FatI CATG 6 cut(s) 268, 346, 382, 540, 580, 687
FokI GGATG 2 cut(s) 140, 443
FspBI CTAG 2 cut(s) 144, 204
HaeIII GGCC 3 cut(s) 35, 359, 365
HapII CCGG 1 cut(s) 36
HgaI GACGC 2 cut(s) 348, 392
Hin1I GRCGYC 1 cut(s) 340
Hin1II CATG 6 cut(s) 272, 350, 386, 544, 584, 691
HinfI GANTC 1 cut(s) 281
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 446
Hpy188I TCNGA 3 cut(s) 112, 120, 520
Hpy188III TCNNGA 1 cut(s) 680
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 3 cut(s) 96, 397, 409
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 500
HpyF3I CTNAG 1 cut(s) 335
HpySE526I ACGT 1 cut(s) 600
Hsp92I GRCGYC 1 cut(s) 340
Hsp92II CATG 6 cut(s) 272, 350, 386, 544, 584, 691
Kzo9I GATC 2 cut(s) 136, 534
LmnI GCTCC 2 cut(s) 103, 709
LweI GCATC 4 cut(s) 118, 279, 481, 701
MaeI CTAG 2 cut(s) 144, 204
MaeII ACGT 1 cut(s) 600
MaeIII GTNAC 3 cut(s) 78, 174, 282
MalI GATC 2 cut(s) 138, 536
MboI GATC 2 cut(s) 136, 534
MboII GAAGA 6 cut(s) 248, 253, 449, 485, 560, 626
MfeI CAATTG 1 cut(s) 97
MflI RGATCY 1 cut(s) 534
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 4 cut(s) 97, 276, 443, 675
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 237
MroXI GAANNNNTTC 1 cut(s) 53
MslI CAYNNNNRTG 1 cut(s) 585
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 32
MunI CAATTG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 2 cut(s) 269, 500
NcoI CCATGG 1 cut(s) 687
NdeII GATC 2 cut(s) 136, 534
NlaIII CATG 6 cut(s) 272, 350, 386, 544, 584, 691
NlaIV GGNNCC 2 cut(s) 6, 162
NmuCI GTSAC 2 cut(s) 78, 282
NspI RCATGY 1 cut(s) 272
PceI AGGCCT 1 cut(s) 365
PdmI GAANNNNTTC 1 cut(s) 53
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 6, 162
PsuI RGATCY 1 cut(s) 534
RsaI GTAC 2 cut(s) 301, 646
RsaNI GTAC 2 cut(s) 300, 645
RseI CAYNNNNRTG 1 cut(s) 585
Sau3AI GATC 2 cut(s) 136, 534
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 4 cut(s) 118, 279, 481, 701
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 4 cut(s) 97, 276, 443, 675
SseBI AGGCCT 1 cut(s) 365
SspMI CTAG 2 cut(s) 144, 204
StuI AGGCCT 1 cut(s) 365
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 3 cut(s) 306, 360, 687
TaiI ACGT 1 cut(s) 603
TasI AATT 4 cut(s) 97, 276, 443, 675
TseFI GTSAC 2 cut(s) 78, 282
Tsp45I GTSAC 2 cut(s) 78, 282
TspDTI ATGAA 4 cut(s) 30, 62, 549, 603
XapI RAATTY 1 cut(s) 675
XceI RCATGY 1 cut(s) 272
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 2 cut(s) 144, 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.