RLG00000024161

Cactus-binding C-terminus of cactin protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003428
Physical Location & Seq
Forward (+)
32650221 .. 32655572
5352 bp
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UTR
Exon/CDS
Intron
RLM00000024161

Sequence Viewer

Length: 1470 bp
ATGTCAGAAGCAAACCCTCCTTCAGCTATGACTTCTCCTGTCCTAAAGCCAGAGACACGCAACCCGAATTCTCCTACACCCTCCTCAATCTCCAAGAAACAAGCTCGCAGATTCTCTCTTCCTGATCAAAGACCTATCAAGAAGCAGAAATCCCATCGTGTCCATTATGACCCTAGGCCCAATGAAGCAGAAGAAAAAAACTATTGTTTTGGTAAGATGACCCTAGGCCCAAATAAGTGGAAACCCCAAGCTGTGCTTTATGAACGTAGGCCCAAAACAAGAAAAAAAAAATACACAAACAAAACCCAACTGTCTATTGATATTCCATATTGGCGTCCTCTAACAAAGCCATATTGGAATCCTTTCACTGACAGTATTTCTGGAGACTGGAGCGAGACTAGCAGTGGAGGAAGTAATGATGAAGGACTCGAGCTCGAGTTGATGAAACTAAAGCAAGAAAAGGATCTAACAGCTGATAGAATTGATTTCGAGAATCAAGCTGCGATAGAAAAGGTTAAGAAGAGAAGGGAGGAAAGAGCACTTGAAAAAGCACAACATGAGGAAGAAATGGCGCTGCTTGCCAGAGAACGCACTCGAGCTGAGTTCCAAGACTGGGAGAAAAAAGAAGAAGAGTTTCACTTTGATCAAAGCAAAATGAGGTCAGAGATCAGATTGCGTGAAGGGCATATTAAGCCAATTGATGTACTTTCCAAGCATCGCGATGGCTCAGCTGATTTAGATATTGAGATAAATGAGCCATACATGGTTTTCAAGGGCTTGACAGTAAAGGAATTGGAAGAGCTTCGAGATGACATCAAAATGCATCTTGGTTTGGACAGGGCTACACCTACACATATAGGATACTGGGAGTCACTTTTGGTGGTTTGCAATTGGGAGCTATCAGAAGCTCGGAAAAAGGATGCTTTGGATCAAGCTAGGGTTTGTTGGGAGGAACCTCCTGCCAAGTTACTTGCTGAACAAAGAGGCTTGTATTCTAGCATTGAAGCAGACTTCAAGTATCTCTTGGAAGAGAAGAATTATAGTCAGTTGGAGGCATTACATGCTGAAATTGAGTCACAGATGCGTTCTGGTACAGCCAAGGTTGTTGCGTATTGGGAAGCTGTTCTTAGATGCCTCCATGTATTCAAGGCCAAGGCCTGTTTGAAAGAAATCCATGCTAAAATGTTGCACAAGCGTCTGCAACACCTTGAGGGGCGAGAGGATGGTGAAGAGAAATTGGATATGCCCGATGATTTACAACCTGAAGAGGAGAGTGAACCTGATGCTAATGGTGCTGAAACATATACATCAGAACCCGTAGAGGAGGAGATCCACATGGCTGAAGAGGCCGGTTCATTTTCACAAGAACTATTACATGGAGATGAAAATGAGGACGTGAATGACCCTGAAGAGAATAAGGCTATACTGGTAAGCCTTTTAATAATAAGTGAGCATGGAAAGTTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CactinC_cactus Cactin_mid

Protein Analysis

490

Amino Acids

56.69

Weight (kDa)

5.39

Isoelectric Point (pI)

62.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cactin_mid PF10312 195 - 392 1.6e-55 Conserved mid region of cactin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 720
AclWI GGATC 3 cut(s) 471, 936, 1324
AcsI RAATTY 1 cut(s) 67
AcuI CTGAAG 4 cut(s) 6, 1284, 1362, 1428
AcyI GRCGYC 1 cut(s) 334
AfaI GTAC 2 cut(s) 705, 1093
AfiI CCNNNNNNNGG 1 cut(s) 613
AgsI TTSAA 6 cut(s) 545, 772, 1004, 1015, 1147, 1165
AjiI CACGTC 1 cut(s) 1396
Alw21I GWGCWC 2 cut(s) 435, 541
Alw26I GTCTC 3 cut(s) 47, 378, 389
AlwI GGATC 3 cut(s) 471, 936, 1324
Ama87I CYCGRG 3 cut(s) 428, 434, 594
AoxI GGCC 6 cut(s) 176, 226, 269, 1149, 1155, 1347
ApeKI GCWGC 2 cut(s) 500, 574
ApoI RAATTY 1 cut(s) 67
Asp700I GAANNNNTTC 5 cut(s) 362, 633, 801, 1122, 1462
AspA2I CCTAGG 2 cut(s) 173, 223
AspLEI GCGC 1 cut(s) 574
AspS9I GGNCC 3 cut(s) 177, 227, 270
AsuHPI GGTGA 1 cut(s) 1238
AvaI CYCGRG 3 cut(s) 428, 434, 594
AvrII CCTAGG 2 cut(s) 173, 223
BanII GRGCYC 1 cut(s) 435
Bbv12I GWGCWC 2 cut(s) 435, 541
BbvI GCAGC 2 cut(s) 487, 561
BccI CCATC 3 cut(s) 162, 716, 1217
BciVI GTATCC 1 cut(s) 854
BclI TGATCA 2 cut(s) 124, 643
BcoDI GTCTC 3 cut(s) 47, 378, 389
BfaI CTAG 5 cut(s) 174, 224, 399, 936, 996
BfoI RGCGCY 1 cut(s) 575
BfuI GTATCC 1 cut(s) 854
BisI GCNGC 2 cut(s) 501, 575
BlnI CCTAGG 2 cut(s) 173, 223
BlpI GCTNAGC 1 cut(s) 727
BlsI GCNGC 2 cut(s) 502, 576
BmeT110I CYCGRG 3 cut(s) 428, 434, 594
BmgBI CACGTC 1 cut(s) 1396
BmgT120I GGNCC 3 cut(s) 177, 227, 270
BmiI GGNNCC 1 cut(s) 954
BmrI ACTGGG 2 cut(s) 622, 874
BmsI GCATC 6 cut(s) 724, 832, 910, 1071, 1121, 1273
BmuI ACTGGG 2 cut(s) 622, 874
BpmI CTGGAG 2 cut(s) 402, 409
Bpu1102I GCTNAGC 1 cut(s) 727
BpuEI CTTGAG 1 cut(s) 1229
BsaHI GRCGYC 1 cut(s) 334
BsaJI CCNNGG 4 cut(s) 173, 223, 1098, 1152
Bsc4I CCNNNNNNNGG 1 cut(s) 613
Bse118I RCCGGY 1 cut(s) 1349
Bse1I ACTGG 4 cut(s) 392, 617, 869, 1431
BseDI CCNNGG 4 cut(s) 173, 223, 1098, 1152
BseGI GGATG 2 cut(s) 925, 1228
BseLI CCNNNNNNNGG 1 cut(s) 613
BseMII CTCAG 2 cut(s) 591, 741
BseNI ACTGG 4 cut(s) 392, 617, 869, 1431
BseRI GAGGAG 4 cut(s) 73, 1283, 1337, 1340
BseXI GCAGC 2 cut(s) 487, 561
Bsh1236I CGCG 1 cut(s) 720
BshFI GGCC 6 cut(s) 178, 228, 271, 1151, 1157, 1349
BsiHKAI GWGCWC 2 cut(s) 435, 541
BsiHKCI CYCGRG 3 cut(s) 428, 434, 594
BsiSI CCGG 1 cut(s) 1350
BslI CCNNNNNNNGG 1 cut(s) 613
BsmAI GTCTC 3 cut(s) 47, 378, 389
BsnI GGCC 6 cut(s) 178, 228, 271, 1151, 1157, 1349
BsoBI CYCGRG 3 cut(s) 428, 434, 594
Bsp1286I GDGCHC 2 cut(s) 435, 541
Bsp143I GATC 6 cut(s) 124, 463, 643, 666, 928, 1329
Bsp1720I GCTNAGC 1 cut(s) 727
Bsp68I TCGCGA 1 cut(s) 720
BspANI GGCC 6 cut(s) 178, 228, 271, 1151, 1157, 1349
BspCNI CTCAG 2 cut(s) 592, 740
BspFNI CGCG 1 cut(s) 720
BspLI GGNNCC 1 cut(s) 954
BspPI GGATC 3 cut(s) 471, 936, 1324
BspQI GCTCTTC 1 cut(s) 792
BsrFI RCCGGY 1 cut(s) 1349
BsrI ACTGG 4 cut(s) 392, 617, 869, 1431
BssAI RCCGGY 1 cut(s) 1349
BssECI CCNNGG 4 cut(s) 173, 223, 1098, 1152
BssMI GATC 6 cut(s) 124, 463, 643, 666, 928, 1329
BssNI GRCGYC 1 cut(s) 334
BssT1I CCWWGG 4 cut(s) 173, 223, 1098, 1152
Bst4CI ACNGT 3 cut(s) 312, 374, 784
Bst6I CTCTTC 9 cut(s) 123, 515, 624, 792, 1023, 1224, 1260, 1338, 1404
BstACI GRCGYC 1 cut(s) 334
BstAPI GCANNNNNTGC 1 cut(s) 1061
BstC8I GCNNGC 2 cut(s) 106, 579
BstDEI CTNAG 4 cut(s) 600, 727, 1127, 1467
BstF5I GGATG 2 cut(s) 925, 1228
BstFNI CGCG 1 cut(s) 720
BstH2I RGCGCY 1 cut(s) 575
BstHHI GCGC 1 cut(s) 574
BstKTI GATC 6 cut(s) 127, 466, 646, 669, 931, 1332
BstMAI GTCTC 3 cut(s) 47, 378, 389
BstMBI GATC 6 cut(s) 124, 463, 643, 666, 928, 1329
BstMWI GCNNNNNNNGC 7 cut(s) 399, 578, 682, 691, 1061, 1292, 1346
BstNSI RCATGY 1 cut(s) 1064
BstUI CGCG 1 cut(s) 720
BstV1I GCAGC 2 cut(s) 487, 561
BstX2I RGATCY 2 cut(s) 463, 1329
BstXI CCANNNNNNTGG 1 cut(s) 237
BstYI RGATCY 2 cut(s) 463, 1329
BsuI GTATCC 1 cut(s) 854
BsuRI GGCC 6 cut(s) 178, 228, 271, 1151, 1157, 1349
BtgZI GCGATG 2 cut(s) 701, 735
BtrI CACGTC 1 cut(s) 1396
BtsCI GGATG 2 cut(s) 925, 1228
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 2 cut(s) 366, 409
BtuMI TCGCGA 1 cut(s) 720
Cac8I GCNNGC 2 cut(s) 106, 579
CfoI GCGC 1 cut(s) 574
Cfr10I RCCGGY 1 cut(s) 1349
Cfr13I GGNCC 3 cut(s) 177, 227, 270
CseI GACGC 2 cut(s) 323, 1184
Csp6I GTAC 2 cut(s) 704, 1092
CviAII CATG 8 cut(s) 557, 763, 1061, 1139, 1175, 1336, 1376, 1454
CviQI GTAC 2 cut(s) 704, 1092
DdeI CTNAG 4 cut(s) 600, 727, 1127, 1467
DpnI GATC 6 cut(s) 126, 465, 645, 668, 930, 1331
DpnII GATC 6 cut(s) 124, 463, 643, 666, 928, 1329
Eam1104I CTCTTC 9 cut(s) 123, 515, 624, 792, 1023, 1224, 1260, 1338, 1404
EarI CTCTTC 9 cut(s) 123, 515, 624, 792, 1023, 1224, 1260, 1338, 1404
Ecl136II GAGCTC 1 cut(s) 433
Eco130I CCWWGG 4 cut(s) 173, 223, 1098, 1152
Eco147I AGGCCT 1 cut(s) 1157
Eco24I GRGCYC 1 cut(s) 435
Eco53kI GAGCTC 1 cut(s) 433
Eco57I CTGAAG 4 cut(s) 6, 1284, 1362, 1428
Eco88I CYCGRG 3 cut(s) 428, 434, 594
EcoICRI GAGCTC 1 cut(s) 433
EcoRI GAATTC 1 cut(s) 67
EcoT14I CCWWGG 4 cut(s) 173, 223, 1098, 1152
EcoT22I ATGCAT 1 cut(s) 825
EcoT38I GRGCYC 1 cut(s) 435
ErhI CCWWGG 4 cut(s) 173, 223, 1098, 1152
FaeI CATG 8 cut(s) 560, 766, 1064, 1142, 1178, 1339, 1379, 1457
FalI AAGNNNNNCTT 6 cut(s) 240, 272, 1007, 1039, 1110, 1142
FatI CATG 8 cut(s) 556, 762, 1060, 1138, 1174, 1335, 1375, 1453
FbaI TGATCA 2 cut(s) 124, 643
Fnu4HI GCNGC 2 cut(s) 501, 575
FokI GGATG 2 cut(s) 932, 1235
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 2 cut(s) 501, 575
FspBI CTAG 5 cut(s) 174, 224, 399, 936, 996
GlaI GCGC 1 cut(s) 573
GluI GCNGC 2 cut(s) 501, 575
GsuI CTGGAG 2 cut(s) 402, 409
HaeII RGCGCY 1 cut(s) 575
HaeIII GGCC 6 cut(s) 178, 228, 271, 1151, 1157, 1349
HapII CCGG 1 cut(s) 1350
HgaI GACGC 2 cut(s) 323, 1184
HhaI GCGC 1 cut(s) 574
Hin1I GRCGYC 1 cut(s) 334
Hin1II CATG 8 cut(s) 560, 766, 1064, 1142, 1178, 1339, 1379, 1457
Hin6I GCGC 1 cut(s) 572
HinP1I GCGC 1 cut(s) 572
HinfI GANTC 6 cut(s) 111, 358, 426, 493, 869, 1073
HpaII CCGG 1 cut(s) 1350
HphI GGTGA 1 cut(s) 1238
Hpy166II GTNNAC 1 cut(s) 1277
Hpy188I TCNGA 6 cut(s) 7, 664, 671, 904, 912, 1312
Hpy188III TCNNGA 6 cut(s) 122, 139, 381, 490, 719, 806
Hpy8I GTNNAC 1 cut(s) 1277
HpyAV CCTTC 4 cut(s) 30, 416, 519, 674
HpyCH4III ACNGT 3 cut(s) 312, 374, 784
HpyCH4IV ACGT 2 cut(s) 265, 1395
HpyCH4V TGCA 4 cut(s) 823, 888, 1189, 1201
HpyF10VI GCNNNNNNNGC 7 cut(s) 399, 578, 682, 691, 1061, 1292, 1346
HpyF3I CTNAG 4 cut(s) 600, 727, 1127, 1467
HpySE526I ACGT 2 cut(s) 265, 1395
Hsp92I GRCGYC 1 cut(s) 334
Hsp92II CATG 8 cut(s) 560, 766, 1064, 1142, 1178, 1339, 1379, 1457
HspAI GCGC 1 cut(s) 572
Ksp22I TGATCA 2 cut(s) 124, 643
Kzo9I GATC 6 cut(s) 124, 463, 643, 666, 928, 1329
LguI GCTCTTC 1 cut(s) 792
LmnI GCTCC 2 cut(s) 390, 895
Lsp1109I GCAGC 2 cut(s) 487, 561
LweI GCATC 6 cut(s) 724, 832, 910, 1071, 1121, 1273
MaeI CTAG 5 cut(s) 174, 224, 399, 936, 996
MaeII ACGT 2 cut(s) 265, 1395
MaeIII GTNAC 3 cut(s) 870, 966, 1074
MalI GATC 6 cut(s) 126, 465, 645, 668, 930, 1331
MboI GATC 6 cut(s) 124, 463, 643, 666, 928, 1329
MfeI CAATTG 2 cut(s) 696, 889
MflI RGATCY 2 cut(s) 463, 1329
MhlI GDGCHC 2 cut(s) 435, 541
MluCI AATT 8 cut(s) 67, 480, 696, 791, 889, 1036, 1068, 1235
MlyI GAGTC 3 cut(s) 420, 878, 1082
MmeI TCCRAC 1 cut(s) 1029
Mph1103I ATGCAT 1 cut(s) 825
MroXI GAANNNNTTC 5 cut(s) 362, 633, 801, 1122, 1462
MseI TTAA 3 cut(s) 516, 690, 1439
MslI CAYNNNNRTG 3 cut(s) 720, 818, 1380
MspA1I CMGCKG 2 cut(s) 473, 731
MspI CCGG 1 cut(s) 1350
MunI CAATTG 2 cut(s) 696, 889
MvnI CGCG 1 cut(s) 720
MwoI GCNNNNNNNGC 7 cut(s) 399, 578, 682, 691, 1061, 1292, 1346
NdeII GATC 6 cut(s) 124, 463, 643, 666, 928, 1329
NlaIII CATG 8 cut(s) 560, 766, 1064, 1142, 1178, 1339, 1379, 1457
NlaIV GGNNCC 1 cut(s) 954
NmuCI GTSAC 2 cut(s) 870, 1074
NruI TCGCGA 1 cut(s) 720
NsiI ATGCAT 1 cut(s) 825
NspI RCATGY 1 cut(s) 1064
PaeR7I CTCGAG 3 cut(s) 428, 434, 594
PceI AGGCCT 1 cut(s) 1157
PciSI GCTCTTC 1 cut(s) 792
PdmI GAANNNNTTC 5 cut(s) 362, 633, 801, 1122, 1462
PfeI GAWTC 3 cut(s) 111, 358, 493
PkrI GCNGC 2 cut(s) 502, 576
PleI GAGTC 3 cut(s) 420, 877, 1081
PpsI GAGTC 3 cut(s) 420, 877, 1081
Psp124BI GAGCTC 1 cut(s) 435
PspN4I GGNNCC 1 cut(s) 954
PspPI GGNCC 3 cut(s) 177, 227, 270
PspXI VCTCGAGB 3 cut(s) 428, 434, 594
PsuI RGATCY 2 cut(s) 463, 1329
PvuII CAGCTG 2 cut(s) 473, 731
RruI TCGCGA 1 cut(s) 720
RsaI GTAC 2 cut(s) 705, 1093
RsaNI GTAC 2 cut(s) 704, 1092
RseI CAYNNNNRTG 3 cut(s) 720, 818, 1380
SacI GAGCTC 1 cut(s) 435
SapI GCTCTTC 1 cut(s) 792
SaqAI TTAA 3 cut(s) 516, 690, 1439
SatI GCNGC 2 cut(s) 501, 575
Sau3AI GATC 6 cut(s) 124, 463, 643, 666, 928, 1329
Sau96I GGNCC 3 cut(s) 177, 227, 270
SchI GAGTC 3 cut(s) 420, 878, 1082
SduI GDGCHC 2 cut(s) 435, 541
SfaNI GCATC 6 cut(s) 724, 832, 910, 1071, 1121, 1273
Sfr274I CTCGAG 3 cut(s) 428, 434, 594
SlaI CTCGAG 3 cut(s) 428, 434, 594
SmiMI CAYNNNNRTG 3 cut(s) 720, 818, 1380
SmlI CTYRAG 4 cut(s) 428, 434, 594, 1208
SmoI CTYRAG 4 cut(s) 428, 434, 594, 1208
Sse9I AATT 8 cut(s) 67, 480, 696, 791, 889, 1036, 1068, 1235
SseBI AGGCCT 1 cut(s) 1157
SspMI CTAG 5 cut(s) 174, 224, 399, 936, 996
SstI GAGCTC 1 cut(s) 435
StuI AGGCCT 1 cut(s) 1157
StyI CCWWGG 4 cut(s) 173, 223, 1098, 1152
TaaI ACNGT 3 cut(s) 312, 374, 784
TaiI ACGT 2 cut(s) 268, 1398
TaqI TCGA 5 cut(s) 429, 435, 489, 595, 805
TasI AATT 8 cut(s) 67, 480, 696, 791, 889, 1036, 1068, 1235
TatI WGTACW 1 cut(s) 703
TfiI GAWTC 3 cut(s) 111, 358, 493
Tru1I TTAA 3 cut(s) 516, 690, 1439
Tru9I TTAA 3 cut(s) 516, 690, 1439
TscAI CASTG 2 cut(s) 373, 409
TseFI GTSAC 2 cut(s) 870, 1074
TseI GCWGC 2 cut(s) 500, 574
Tsp45I GTSAC 2 cut(s) 870, 1074
TspDTI ATGAA 6 cut(s) 198, 276, 435, 458, 1344, 1398
TspRI CASTG 2 cut(s) 373, 409
XapI RAATTY 1 cut(s) 67
XceI RCATGY 1 cut(s) 1064
XhoI CTCGAG 3 cut(s) 428, 434, 594
XmaJI CCTAGG 2 cut(s) 173, 223
XmnI GAANNNNTTC 5 cut(s) 362, 633, 801, 1122, 1462
XspI CTAG 5 cut(s) 174, 224, 399, 936, 996
Zsp2I ATGCAT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.