Rh3BG194800

Cactus-binding C-terminus of cactin protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0003428
Physical Location & Seq
Reverse (-)
17222937 .. 17224328
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG194800.1

Sequence Viewer

Length: 597 bp
ATGCTTTGGATCAAGCTAGGGTTCGTTGGGAGGAACCTCCTGCCAAGTTACTTGCTGAACAAAGAGGCTTATATTCTAGCATTGAAGCAGACTTCCAAGTATCTCTTGGAAGAGAAGAATTATAGTCAGTTGGAGGCATTACATGCTGAAATTGAGTCACAGATGCGTTCTGGTACAGCCAAGGTTGTTGCGTATTGGGAGGCTGTTCTTAGACGCCTCCATGTATTGAAGGCCAAGGCCTGTTTGAAAGAAATCCATGCTAAAATGTTGCACAAGCGTCTGCAACACCTTGAGGGGCGAGAGGATGGTGAAGAGAAATTGGATATGCCCGATGATTTACAACCTGAAGAGGAGAGTGAGCCTGATGCTAATGGTGCTGAAACATATACATCAGAACCCGTAGAGGAGATCCACATGGCTGAAGAGGCCGGTTCATTTTCACAAGAACTATTACATGGAGATGAAAATGAGGACGTGAATGACCCTGAAGAGAATACGGCTATACTGGAACACAAGCGTACAGCTTTATTAGAACAACAACAACCACGAATTCAAGAAGCTATGGCATCAAAGCCAGCTCCACCACCAGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CactinC_cactus Cactin_mid

Protein Analysis

198

Amino Acids

22.59

Weight (kDa)

4.84

Isoelectric Point (pI)

75.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cactin_mid PF10312 30 - 86 9.1e-14 Conserved mid region of cactin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 17, 403
AcsI RAATTY 1 cut(s) 549
AcuI CTGAAG 3 cut(s) 366, 441, 507
AcyI GRCGYC 1 cut(s) 214
AfaI GTAC 2 cut(s) 175, 520
AgsI TTSAA 4 cut(s) 85, 229, 247, 554
AjiI CACGTC 1 cut(s) 475
AluBI AGCT 4 cut(s) 16, 524, 560, 578
AluI AGCT 4 cut(s) 16, 524, 560, 578
AlwI GGATC 2 cut(s) 17, 403
AoxI GGCC 3 cut(s) 231, 237, 426
ApoI RAATTY 1 cut(s) 549
AsuHPI GGTGA 1 cut(s) 320
BccI CCATC 1 cut(s) 299
BceAI ACGGC 1 cut(s) 513
BfaI CTAG 2 cut(s) 17, 77
BmgBI CACGTC 1 cut(s) 475
BmiI GGNNCC 1 cut(s) 35
BmsI GCATC 3 cut(s) 153, 355, 575
BpuEI CTTGAG 1 cut(s) 311
BsaHI GRCGYC 1 cut(s) 214
BsaJI CCNNGG 2 cut(s) 180, 234
Bse118I RCCGGY 1 cut(s) 428
Bse1I ACTGG 1 cut(s) 510
BseDI CCNNGG 2 cut(s) 180, 234
BseGI GGATG 1 cut(s) 310
BseNI ACTGG 1 cut(s) 510
BseRI GAGGAG 2 cut(s) 365, 419
BshFI GGCC 3 cut(s) 233, 239, 428
BsiSI CCGG 1 cut(s) 429
BsnI GGCC 3 cut(s) 233, 239, 428
Bsp143I GATC 2 cut(s) 9, 408
BspANI GGCC 3 cut(s) 233, 239, 428
BspLI GGNNCC 1 cut(s) 35
BspPI GGATC 2 cut(s) 17, 403
BsrFI RCCGGY 1 cut(s) 428
BsrI ACTGG 1 cut(s) 510
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 2 cut(s) 180, 234
BssMI GATC 2 cut(s) 9, 408
BssNI GRCGYC 1 cut(s) 214
BssT1I CCWWGG 2 cut(s) 180, 234
Bst6I CTCTTC 5 cut(s) 105, 306, 342, 417, 483
BstACI GRCGYC 1 cut(s) 214
BstAPI GCANNNNNTGC 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 576
BstDEI CTNAG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 310
BstKTI GATC 2 cut(s) 12, 411
BstMBI GATC 2 cut(s) 9, 408
BstMWI GCNNNNNNNGC 3 cut(s) 143, 374, 425
BstNSI RCATGY 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 408
BstYI RGATCY 1 cut(s) 408
BsuRI GGCC 3 cut(s) 233, 239, 428
BtrI CACGTC 1 cut(s) 475
BtsCI GGATG 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 576
Cfr10I RCCGGY 1 cut(s) 428
CseI GACGC 2 cut(s) 222, 266
Csp6I GTAC 2 cut(s) 174, 519
CviAII CATG 5 cut(s) 143, 221, 257, 415, 455
CviQI GTAC 2 cut(s) 174, 519
DdeI CTNAG 1 cut(s) 209
DpnI GATC 2 cut(s) 11, 410
DpnII GATC 2 cut(s) 9, 408
Eam1104I CTCTTC 5 cut(s) 105, 306, 342, 417, 483
EarI CTCTTC 5 cut(s) 105, 306, 342, 417, 483
Eco130I CCWWGG 2 cut(s) 180, 234
Eco147I AGGCCT 1 cut(s) 239
Eco57I CTGAAG 3 cut(s) 366, 441, 507
EcoRI GAATTC 1 cut(s) 549
EcoT14I CCWWGG 2 cut(s) 180, 234
ErhI CCWWGG 2 cut(s) 180, 234
FaeI CATG 5 cut(s) 146, 224, 260, 418, 458
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FatI CATG 5 cut(s) 142, 220, 256, 414, 454
FokI GGATG 1 cut(s) 317
FspBI CTAG 2 cut(s) 17, 77
HaeIII GGCC 3 cut(s) 233, 239, 428
HapII CCGG 1 cut(s) 429
HgaI GACGC 2 cut(s) 222, 266
Hin1I GRCGYC 1 cut(s) 214
Hin1II CATG 5 cut(s) 146, 224, 260, 418, 458
HinfI GANTC 1 cut(s) 155
HpaII CCGG 1 cut(s) 429
HphI GGTGA 1 cut(s) 320
Hpy188I TCNGA 1 cut(s) 394
Hpy188III TCNNGA 1 cut(s) 554
HpyAV CCTTC 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 2 cut(s) 271, 283
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 374, 425
HpyF3I CTNAG 1 cut(s) 209
HpySE526I ACGT 1 cut(s) 474
Hsp92I GRCGYC 1 cut(s) 214
Hsp92II CATG 5 cut(s) 146, 224, 260, 418, 458
Kzo9I GATC 2 cut(s) 9, 408
LmnI GCTCC 1 cut(s) 583
LpnPI CCDG 9 cut(s) 53, 156, 253, 357, 375, 442, 491, 498, 588
LweI GCATC 3 cut(s) 153, 355, 575
MaeI CTAG 2 cut(s) 17, 77
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 2 cut(s) 47, 156
MalI GATC 2 cut(s) 11, 410
MboI GATC 2 cut(s) 9, 408
MboII GAAGA 6 cut(s) 122, 127, 323, 359, 434, 500
MflI RGATCY 1 cut(s) 408
MluCI AATT 4 cut(s) 118, 150, 317, 549
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 459
MspI CCGG 1 cut(s) 429
MwoI GCNNNNNNNGC 3 cut(s) 143, 374, 425
NdeII GATC 2 cut(s) 9, 408
NlaIII CATG 5 cut(s) 146, 224, 260, 418, 458
NlaIV GGNNCC 1 cut(s) 35
NmuCI GTSAC 1 cut(s) 156
NspI RCATGY 1 cut(s) 146
PceI AGGCCT 1 cut(s) 239
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 35
PsuI RGATCY 1 cut(s) 408
RsaI GTAC 2 cut(s) 175, 520
RsaNI GTAC 2 cut(s) 174, 519
RseI CAYNNNNRTG 1 cut(s) 459
Sau3AI GATC 2 cut(s) 9, 408
SchI GAGTC 1 cut(s) 164
SetI ASST 9 cut(s) 18, 39, 186, 291, 346, 477, 526, 562, 580
SfaNI GCATC 3 cut(s) 153, 355, 575
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 4 cut(s) 118, 150, 317, 549
SseBI AGGCCT 1 cut(s) 239
SspMI CTAG 2 cut(s) 17, 77
StuI AGGCCT 1 cut(s) 239
StyI CCWWGG 2 cut(s) 180, 234
TaiI ACGT 1 cut(s) 477
TasI AATT 4 cut(s) 118, 150, 317, 549
TseFI GTSAC 1 cut(s) 156
Tsp45I GTSAC 1 cut(s) 156
TspDTI ATGAA 2 cut(s) 423, 477
XapI RAATTY 1 cut(s) 549
XceI RCATGY 1 cut(s) 146
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XspI CTAG 2 cut(s) 17, 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.