MD14G1127700.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015077
Physical Location & Seq
Reverse (-)
20333685 .. 20334662
978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1127700.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGACATAATGCATGAAAACAACCTTGCTTTTGGGTTCACAGACGCCATTGACATCCGATCTTCTCTCTCAAATCTCATACTAACAAGCTCCACAAACACCCTGGACTCGATTTTCTCTCACTGCCCTCAAACACTCGCCGCCGCCGTAACAACCACCACCAGCGGCAGTTCTGTATCTGAGCCGCTGGGCTCATCAGTTTACCTCCGCCAGAGAGACCTCCTCCAGAAGTTCTGCGACGAAGGCCGAGCAAAAGTAAACACCACGGCCTCGCGCTCTTTTGCGACGAACCCATTTCAGAGTTCGGTTTGTTCTTCGACTACTTACATGGCTCCATGCAAGAAGAAGCTCTACAGGGGAGTACGGCAGAGGCATTGGGGCAAATGGGTCGCCGAGATCAGACTCCCTCAGAACAGAATGAGAGTCTGGCTCGGCACCTACGACACCGCGGAAGCCGCCGCCTACGCTTACGACCGGGCGGCCTACAAGCTCCGCGGTGAGTACGCGAAGTTGAATTTCCCCAATCTCAAGGACCCGAACAATTTGGGTTTTGGCGACTGGTCTAGACTGAACGCTGTGAAAAACTCCGTGGATGCGAAAATCCAAGCTATTTGTCAGAAGGTGAAGAGAGAAAGAGCAAAGAAGAACGTGAAGAAGAGCAGCTCTTCAGTTGGTGGTGATGGGAGTTTCCCTGAGAACGGGAAGGTTGTGAAGATGGTATCCTCGCCGTCGTCCTCATCGTCGCCTTTGGTCGATGGCGAAGGTTTTGGTCACGAAATGGTGTCATTAACGCCCACGCCCTCCGTGTCTGAGGACGGGGTTTGGAGGATTGAGAACCCGCCGCCGTCTGTGTCAACAGAGTTTCAAATGGGGATGGCGGAGTTGGGTATGGGAGAGAAGTCGGAATTTGAGGGGTGTTCACTTGCCAAAATGCCTTCATTTGATCCAGAGTTGATTTGGGAAGTTCTTGCTATTTAG

Protein Analysis

326

Amino Acids

35.65

Weight (kDa)

8.64

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 117 - 166 7.2e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015077)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64380
fragaria_vesca FvH4_5g04470
malus_domestica MD06G1105500.v1.1 MD14G1127700.v1.1
prunus_persica Prupe.5G117800_v2.0.a1
pyrus_communis pycom06g09980
rosa_chinensis RchiOBHm_Chr7g0195031
rosa_laevigata RLG00000004151 RLG00000015304
rosa_multiflora Rmu_sc0002246.1_g000002
rosa_rugosa Rorug07G0020000
rosa_samantha Rh7AG147800 Rh7BG148400 Rh7CG151900 Rh7DG148800
rosa_wichuraiana Rw7G012600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 434
AccII CGCG 4 cut(s) 274, 449, 495, 506
AclWI GGATC 1 cut(s) 938
AcsI RAATTY 2 cut(s) 514, 905
AcuI CTGAAG 1 cut(s) 651
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 363, 503
AfiI CCNNNNNNNGG 1 cut(s) 698
AgsI TTSAA 2 cut(s) 514, 866
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 5 cut(s) 90, 349, 490, 608, 663
AluI AGCT 5 cut(s) 90, 349, 490, 608, 663
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 1 cut(s) 938
AoxI GGCC 3 cut(s) 244, 267, 480
ApeKI GCWGC 1 cut(s) 660
ApoI RAATTY 2 cut(s) 514, 905
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 532
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 3 cut(s) 509, 634, 689
AvaII GGWCC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 434
BanII GRGCYC 1 cut(s) 194
BarI GAAGNNNNNNTAC 2 cut(s) 335, 367
BbvI GCAGC 1 cut(s) 672
BccI CCATC 4 cut(s) 674, 709, 749, 868
BceAI ACGGC 5 cut(s) 131, 282, 380, 712, 829
BcgI CGANNNNNNTGC 2 cut(s) 370, 404
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 1 cut(s) 730
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 1 cut(s) 564
BfmI CTRYAG 1 cut(s) 352
BfuI GTATCC 1 cut(s) 730
BisI GCNGC 9 cut(s) 141, 144, 166, 185, 456, 459, 480, 661, 842
BlsI GCNGC 9 cut(s) 142, 145, 167, 186, 457, 460, 481, 662, 843
Bme1390I CCNGG 2 cut(s) 104, 476
Bme18I GGWCC 1 cut(s) 532
BmgT120I GGNCC 1 cut(s) 532
BmiI GGNNCC 3 cut(s) 333, 436, 534
BmrFI CCNGG 2 cut(s) 104, 476
BmsI GCATC 1 cut(s) 583
BplI GAGNNNNNCTC 4 cut(s) 207, 239, 414, 446
BpmI CTGGAG 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 512
BpuMI CCSGG 1 cut(s) 476
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 210
BsaJI CCNNGG 5 cut(s) 102, 264, 447, 493, 588
Bsc4I CCNNNNNNNGG 1 cut(s) 698
Bse1I ACTGG 1 cut(s) 563
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 5 cut(s) 102, 264, 447, 493, 588
BseGI GGATG 3 cut(s) 54, 598, 879
BseLI CCNNNNNNNGG 1 cut(s) 698
BseMII CTCAG 4 cut(s) 171, 422, 684, 801
BseNI ACTGG 1 cut(s) 563
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 1 cut(s) 672
BseYI CCCAGC 1 cut(s) 187
Bsh1236I CGCG 4 cut(s) 274, 449, 495, 506
Bsh1285I CGRYCG 1 cut(s) 475
BshFI GGCC 3 cut(s) 246, 269, 482
BshNI GGYRCC 1 cut(s) 434
BsiEI CGRYCG 1 cut(s) 475
BsiSI CCGG 1 cut(s) 475
BslI CCNNNNNNNGG 1 cut(s) 698
BsmAI GTCTC 1 cut(s) 210
BsnI GGCC 3 cut(s) 246, 269, 482
Bso31I GGTCTC 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 194
Bsp143I GATC 3 cut(s) 59, 396, 943
BspANI GGCC 3 cut(s) 246, 269, 482
BspCNI CTCAG 4 cut(s) 172, 421, 685, 802
BspFNI CGCG 4 cut(s) 274, 449, 495, 506
BspLI GGNNCC 3 cut(s) 333, 436, 534
BspPI GGATC 1 cut(s) 938
BspQI GCTCTTC 2 cut(s) 650, 670
BspT107I GGYRCC 1 cut(s) 434
BspTNI GGTCTC 1 cut(s) 210
BsrI ACTGG 1 cut(s) 563
BssECI CCNNGG 5 cut(s) 102, 264, 447, 493, 588
BssMI GATC 3 cut(s) 59, 396, 943
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 104
Bst6I CTCTTC 3 cut(s) 620, 650, 670
BstACI GRCGYC 1 cut(s) 45
BstDEI CTNAG 4 cut(s) 180, 408, 693, 810
BstDSI CCRYGG 4 cut(s) 264, 447, 493, 588
BstF5I GGATG 3 cut(s) 54, 598, 879
BstFNI CGCG 4 cut(s) 274, 449, 495, 506
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 3 cut(s) 62, 399, 946
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 3 cut(s) 59, 396, 943
BstMCI CGRYCG 1 cut(s) 475
BstMWI GCNNNNNNNGC 3 cut(s) 243, 455, 464
BstNI CCWGG 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 102, 474
BstSFI CTRYAG 1 cut(s) 352
BstUI CGCG 4 cut(s) 274, 449, 495, 506
BstV1I GCAGC 1 cut(s) 672
BsuI GTATCC 1 cut(s) 730
BsuRI GGCC 3 cut(s) 246, 269, 482
BtgI CCRYGG 4 cut(s) 264, 447, 493, 588
BtsCI GGATG 3 cut(s) 54, 598, 879
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 1 cut(s) 121
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 532
Cfr42I CCGCGG 2 cut(s) 450, 496
CseI GACGC 1 cut(s) 53
Csp6I GTAC 2 cut(s) 362, 502
CviAII CATG 3 cut(s) 14, 328, 336
CviQI GTAC 2 cut(s) 362, 502
DdeI CTNAG 4 cut(s) 180, 408, 693, 810
DpnI GATC 3 cut(s) 61, 398, 945
DpnII GATC 3 cut(s) 59, 396, 943
Eam1104I CTCTTC 3 cut(s) 620, 650, 670
EarI CTCTTC 3 cut(s) 620, 650, 670
EciI GGCGGA 2 cut(s) 197, 893
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 532
Eco57I CTGAAG 1 cut(s) 651
EcoO109I RGGNCCY 1 cut(s) 532
EcoRII CCWGG 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 15
EcoT38I GRGCYC 1 cut(s) 194
FaeI CATG 3 cut(s) 17, 331, 339
FaiI YATR 6 cut(s) 8, 15, 80, 329, 337, 890
FatI CATG 3 cut(s) 13, 327, 335
FauI CCCGC 1 cut(s) 846
Fnu4HI GCNGC 9 cut(s) 141, 144, 166, 185, 456, 459, 480, 661, 842
FokI GGATG 3 cut(s) 41, 605, 886
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 9 cut(s) 141, 144, 166, 185, 456, 459, 480, 661, 842
FspBI CTAG 1 cut(s) 564
GlaI GCGC 1 cut(s) 275
GluI GCNGC 9 cut(s) 141, 144, 166, 185, 456, 459, 480, 661, 842
GsaI CCCAGC 1 cut(s) 191
GsuI CTGGAG 1 cut(s) 209
HaeIII GGCC 3 cut(s) 246, 269, 482
HapII CCGG 1 cut(s) 475
HgaI GACGC 1 cut(s) 53
HhaI GCGC 1 cut(s) 276
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 3 cut(s) 17, 331, 339
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HinfI GANTC 3 cut(s) 107, 402, 423
HpaII CCGG 1 cut(s) 475
HphI GGTGA 3 cut(s) 509, 634, 689
Hpy166II GTNNAC 5 cut(s) 39, 202, 259, 855, 920
Hpy188I TCNGA 8 cut(s) 59, 181, 300, 401, 411, 618, 811, 904
Hpy188III TCNNGA 4 cut(s) 226, 564, 773, 947
Hpy8I GTNNAC 5 cut(s) 39, 202, 259, 855, 920
Hpy99I CGWCG 4 cut(s) 242, 289, 733, 745
HpyAV CCTTC 5 cut(s) 236, 613, 697, 755, 945
HpyCH4IV ACGT 1 cut(s) 648
HpyCH4V TGCA 2 cut(s) 13, 339
HpyF10VI GCNNNNNNNGC 3 cut(s) 243, 455, 464
HpyF3I CTNAG 4 cut(s) 180, 408, 693, 810
HpySE526I ACGT 1 cut(s) 648
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 3 cut(s) 17, 331, 339
HspAI GCGC 1 cut(s) 274
KspI CCGCGG 2 cut(s) 450, 496
Kzo9I GATC 3 cut(s) 59, 396, 943
LguI GCTCTTC 2 cut(s) 650, 670
LmnI GCTCC 3 cut(s) 95, 337, 495
Lsp1109I GCAGC 1 cut(s) 672
LweI GCATC 1 cut(s) 583
MaeI CTAG 1 cut(s) 564
MaeII ACGT 1 cut(s) 648
MaeIII GTNAC 2 cut(s) 148, 770
MalI GATC 3 cut(s) 61, 398, 945
MboI GATC 3 cut(s) 59, 396, 943
MboII GAAGA 9 cut(s) 54, 306, 355, 637, 655, 657, 664, 667, 724
MhlI GDGCHC 1 cut(s) 194
MluCI AATT 3 cut(s) 514, 541, 905
MlyI GAGTC 3 cut(s) 101, 396, 432
MmeI TCCRAC 1 cut(s) 882
Mph1103I ATGCAT 1 cut(s) 15
MseI TTAA 1 cut(s) 788
MspA1I CMGCKG 4 cut(s) 165, 187, 449, 495
MspI CCGG 1 cut(s) 475
MspR9I CCNGG 2 cut(s) 104, 476
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 4 cut(s) 274, 449, 495, 506
MwoI GCNNNNNNNGC 3 cut(s) 243, 455, 464
NciI CCSGG 1 cut(s) 476
NdeII GATC 3 cut(s) 59, 396, 943
NlaIII CATG 3 cut(s) 17, 331, 339
NlaIV GGNNCC 3 cut(s) 333, 436, 534
NmeAIII GCCGAG 3 cut(s) 272, 411, 418
NmuCI GTSAC 1 cut(s) 770
NsiI ATGCAT 1 cut(s) 15
PciSI GCTCTTC 2 cut(s) 650, 670
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 438, 737
PkrI GCNGC 9 cut(s) 142, 145, 167, 186, 457, 460, 481, 662, 843
PleI GAGTC 3 cut(s) 101, 396, 431
PpsI GAGTC 3 cut(s) 101, 396, 431
PpuMI RGGWCCY 1 cut(s) 532
Psp5II RGGWCCY 1 cut(s) 532
Psp6I CCWGG 1 cut(s) 102
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 3 cut(s) 333, 436, 534
PspPI GGNCC 1 cut(s) 532
PspPPI RGGWCCY 1 cut(s) 532
RsaI GTAC 2 cut(s) 363, 503
RsaNI GTAC 2 cut(s) 362, 502
SacII CCGCGG 2 cut(s) 450, 496
SapI GCTCTTC 2 cut(s) 650, 670
SaqAI TTAA 1 cut(s) 788
SatI GCNGC 9 cut(s) 141, 144, 166, 185, 456, 459, 480, 661, 842
Sau3AI GATC 3 cut(s) 59, 396, 943
Sau96I GGNCC 1 cut(s) 532
SchI GAGTC 3 cut(s) 101, 396, 432
ScrFI CCNGG 2 cut(s) 104, 476
SduI GDGCHC 1 cut(s) 194
SfaNI GCATC 1 cut(s) 583
SfcI CTRYAG 1 cut(s) 352
Sfr303I CCGCGG 2 cut(s) 450, 496
SgrBI CCGCGG 2 cut(s) 450, 496
SinI GGWCC 1 cut(s) 532
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
Sse9I AATT 3 cut(s) 514, 541, 905
SspMI CTAG 1 cut(s) 564
StyD4I CCNGG 2 cut(s) 102, 474
TaiI ACGT 1 cut(s) 651
TaqI TCGA 3 cut(s) 110, 317, 753
TasI AATT 3 cut(s) 514, 541, 905
TauI GCSGC 8 cut(s) 143, 146, 168, 187, 458, 461, 482, 844
Tru1I TTAA 1 cut(s) 788
Tru9I TTAA 1 cut(s) 788
TscAI CASTG 1 cut(s) 128
TseFI GTSAC 1 cut(s) 770
TseI GCWGC 1 cut(s) 660
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 2 cut(s) 30, 927
TspGWI ACGGA 2 cut(s) 577, 793
TspRI CASTG 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 532
XapI RAATTY 2 cut(s) 514, 905
XbaI TCTAGA 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 2 cut(s) 100, 954
XspI CTAG 1 cut(s) 564
Zsp2I ATGCAT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.