MD14G1153400.v1.1

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008990
Physical Location & Seq
Reverse (-)
24745512 .. 24747337
1826 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1153400.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGGAAAAGCTTGGACCTTTCTTACCCAACTTCACACGGTTGCTGGGCCAGTGCTGATGTTGCTTTACCCTTTATATGCATCAGTGGTAGCCATAGAGAGCACATCCAAACTAGATGATCAGCAGTGGCTTGCCTATTGGATTATTTACTCTTTCCTCACTCTCATGGAGATGGTGCTCCAACCAGCCCTTGAGTTGTTACCAATTTGGTACAATGTGAAGCTTGTGTTTGTGGCATGGTTGGTTCTGCCACAGTTCAAGGGGGCAGCTTTCTTGTATGAGAGATATGTGAGAGATCAAGTCAGAAAGTATGCAGGGTTTAATAACCATCCCCAATATAACCATCCCCAATCCAGCAAAACATCATCCCCCACTGGCAAAGCAAAGAACAAGTTTGTGCAGTTCATGAACCCCAAGCATGAGCAGGAGGCCTATTGA

Protein Analysis

146

Amino Acids

16.89

Weight (kDa)

9.25

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 24 - 98 6e-27 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 212
AgsI TTSAA 1 cut(s) 259
AluBI AGCT 3 cut(s) 11, 223, 269
AluI AGCT 3 cut(s) 11, 223, 269
Alw21I GWGCWC 2 cut(s) 104, 180
AoxI GGCC 2 cut(s) 47, 429
ApeKI GCWGC 1 cut(s) 266
AspS9I GGNCC 2 cut(s) 15, 47
AvaII GGWCC 1 cut(s) 15
Bbv12I GWGCWC 2 cut(s) 104, 180
BbvI GCAGC 1 cut(s) 278
BccI CCATC 3 cut(s) 166, 336, 351
BclI TGATCA 1 cut(s) 118
BfaI CTAG 1 cut(s) 113
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme18I GGWCC 1 cut(s) 15
BmgT120I GGNCC 2 cut(s) 15, 47
BmsI GCATC 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 212
Bse1I ACTGG 2 cut(s) 50, 379
BseGI GGATG 4 cut(s) 104, 328, 343, 365
BseNI ACTGG 2 cut(s) 50, 379
BseXI GCAGC 1 cut(s) 278
BseYI CCCAGC 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 419
BshFI GGCC 2 cut(s) 49, 431
BsiHKAI GWGCWC 2 cut(s) 104, 180
BsnI GGCC 2 cut(s) 49, 431
Bsp1286I GDGCHC 2 cut(s) 104, 180
Bsp143I GATC 2 cut(s) 118, 295
BspANI GGCC 2 cut(s) 49, 431
BspHI TCATGA 1 cut(s) 405
BsrI ACTGG 2 cut(s) 50, 379
BssMI GATC 2 cut(s) 118, 295
Bst4CI ACNGT 2 cut(s) 40, 255
BstC8I GCNNGC 1 cut(s) 132
BstF5I GGATG 4 cut(s) 104, 328, 343, 365
BstKTI GATC 2 cut(s) 121, 298
BstMBI GATC 2 cut(s) 118, 295
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstV1I GCAGC 1 cut(s) 278
BsuRI GGCC 2 cut(s) 49, 431
BtsCI GGATG 4 cut(s) 104, 328, 343, 365
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 4 cut(s) 57, 90, 131, 372
Cac8I GCNNGC 1 cut(s) 132
CciI TCATGA 1 cut(s) 405
Cfr13I GGNCC 2 cut(s) 15, 47
Csp6I GTAC 1 cut(s) 211
CviAII CATG 4 cut(s) 166, 237, 406, 419
CviJI RGCY 8 cut(s) 11, 49, 92, 130, 188, 223, 269, 431
CviKI_1 RGCY 8 cut(s) 11, 49, 92, 130, 188, 223, 269, 431
CviQI GTAC 1 cut(s) 211
DpnI GATC 2 cut(s) 120, 297
DpnII GATC 2 cut(s) 118, 295
Eco147I AGGCCT 1 cut(s) 431
Eco47I GGWCC 1 cut(s) 15
EcoT22I ATGCAT 1 cut(s) 82
FaeI CATG 4 cut(s) 169, 240, 409, 422
FatI CATG 4 cut(s) 165, 236, 405, 418
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 1 cut(s) 267
FokI GGATG 4 cut(s) 91, 315, 330, 352
Fsp4HI GCNGC 1 cut(s) 267
FspBI CTAG 1 cut(s) 113
GluI GCNGC 1 cut(s) 267
GsaI CCCAGC 1 cut(s) 48
HaeIII GGCC 2 cut(s) 49, 431
Hin1II CATG 4 cut(s) 169, 240, 409, 422
HindIII AAGCTT 2 cut(s) 9, 221
Hpy188I TCNGA 1 cut(s) 305
Hpy188III TCNNGA 1 cut(s) 406
HpyCH4III ACNGT 2 cut(s) 40, 255
HpyCH4V TGCA 3 cut(s) 80, 314, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
Hsp92II CATG 4 cut(s) 169, 240, 409, 422
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 2 cut(s) 118, 295
LmnI GCTCC 1 cut(s) 183
LpnPI CCDG 7 cut(s) 30, 63, 198, 300, 360, 367, 410
Lsp1109I GCAGC 1 cut(s) 278
LweI GCATC 1 cut(s) 89
MaeI CTAG 1 cut(s) 113
MaeIII GTNAC 1 cut(s) 198
MalI GATC 2 cut(s) 120, 297
MboI GATC 2 cut(s) 118, 295
MhlI GDGCHC 2 cut(s) 104, 180
MluCI AATT 1 cut(s) 204
MmeI TCCRAC 1 cut(s) 205
MnlI CCTC 2 cut(s) 167, 421
Mph1103I ATGCAT 1 cut(s) 82
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 2 cut(s) 164, 170
MwoI GCNNNNNNNGC 1 cut(s) 61
NdeII GATC 2 cut(s) 118, 295
NlaIII CATG 4 cut(s) 169, 240, 409, 422
NsiI ATGCAT 1 cut(s) 82
PagI TCATGA 1 cut(s) 405
PceI AGGCCT 1 cut(s) 431
PkrI GCNGC 1 cut(s) 268
PspFI CCCAGC 1 cut(s) 44
PspPI GGNCC 2 cut(s) 15, 47
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
RseI CAYNNNNRTG 2 cut(s) 164, 170
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 2 cut(s) 118, 295
Sau96I GGNCC 2 cut(s) 15, 47
SduI GDGCHC 2 cut(s) 104, 180
SetI ASST 4 cut(s) 13, 20, 225, 271
SfaNI GCATC 1 cut(s) 89
SinI GGWCC 1 cut(s) 15
SmiMI CAYNNNNRTG 2 cut(s) 164, 170
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 1 cut(s) 204
SseBI AGGCCT 1 cut(s) 431
SspMI CTAG 1 cut(s) 113
StuI AGGCCT 1 cut(s) 431
TaaI ACNGT 2 cut(s) 40, 255
TasI AATT 1 cut(s) 204
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 4 cut(s) 57, 90, 131, 379
TseI GCWGC 1 cut(s) 266
TspDTI ATGAA 2 cut(s) 394, 422
TspRI CASTG 4 cut(s) 57, 90, 131, 379
VpaK11BI GGWCC 1 cut(s) 15
XspI CTAG 1 cut(s) 113
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.