Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005644

Physical Location & Seq

Genomic Coordinates Forward (+)

25754177 .. 25756684

Gene Structure 2508 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1163400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1005 bp

ATGTCACGGACGAGAAAGTCCAACAGAAACAAAAAACCCACCACCGTCGAAATCGTCCTCGACTCCTCTGACACCATCACCGACACCGACTCCGACTCCGACTCCTCCGACCTAAACTCTCCAATCTCAGAGCTTTTCAGACAAAATGTGTCGAAACATCGCTCATGCTGGAGGCATGTTTTTGCGTCTTCGATTGTTCAAAAGAAGAGAATAACTCTAGAGGATATTAAAGAGGTAAAGAAGAGTTCAATGTGCCTTTTGGAATGCCCTCCCCGCTTCACTCGCCACGAGAGGTCAAAGAGATTTGGGAAAAAAGAAGAGGTGAAAGAGCTGATGAGGCCTCACAAGGTTGCCGAACGTGCAGTTTCACGAAAAAAGGAAAAGCTGGACTCTGGAGATTTCATGCGTTACTTTGAGAACTTGTGGAACAGCTTTTCAGAAGACAAGAGGACCTCTTTCGCTTACCTTGACTGTTTATGGTTTAGCTTGTACTTGCAACAAAACATTCACAAAGAACTTAAACGAGACATTAAGACCAAGGTGCTGAAGTGGATTAAGAATAAGTGTATCTTCTCAAAGAAATATGTCATTGTTCCTATCGTTTGCTGGAGCCACTGGAACCTCTTAATTCTATGCCATTTTGGTGAGAGTCTGCAGTCGGAAACCCAAAGACCATGTATGCTGTTGCTTGATTCTCTTCAAGGTGCTGATCCGGGGCGGTATGAAGAATATATAAGAAAATTTGTACTGGACATTTTTATGGAAGAGGGAAGACCTGAAAAGAAAGATCTTATCTACCGAATTCCTTTGTTGGTGCCTGAGGTGCCACAACAGCGAGATGATTTCGAGTGTGGTAACTTTGTTCTCTACTACATTAACTTGTTCATGGAAGGTGCTCCCGAGAATTTCAGCACTGAGGGCTACCCCTACTTTATGAAGAAGGATTGGTTTTCCCCAAAGGGCTTGGATTATTTCTGCCAGAAACTGTACACTTCTGCAACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009628 GO:0009651 GO:0009791 GO:0009987 GO:0010228 GO:0016787 GO:0016926 GO:0016929 GO:0018193 GO:0018205 GO:0019538 GO:0019783 GO:0022414 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0070011 GO:0070122 GO:0070137 GO:0070138 GO:0070139 GO:0070140 GO:0070646 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_C48

Protein Analysis

335

Amino Acids

39.67

Weight (kDa)

8.98

Isoelectric Point (pI)

54.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_C48	PF02902	169 - 315	1.8e-12	Ulp1 protease family, C-terminal catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005644)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48480
fragaria_vesca	FvH4_3g25530 FvH4_5g07530
malus_domestica	MD14G1163400.v1.1
prunus_persica	Prupe.5G156800_v2.0.a1 Prupe.5G156800_v2.0.a1
pyrus_communis	pycom14g13600
rosa_chinensis	RchiOBHm_Chr7g0191071
rosa_laevigata	RLG00000004477
rosa_multiflora	Rmu_co8255195.1_g000001 Rmu_sc0005903.1_g000007
rosa_roxburghii	Rroxscaffold_3G00264060
rosa_rugosa	Rorug01G0316800 Rorug01G0316900 Rorug01G0316900 Rorug06G0508100 Rorug06G0508200
rosa_samantha	Rh1CG137300 Rh1DG152400 Rh1DG152500 Rh7AG115700 Rh7AG115800 Rh7BG117700 Rh7BG117800 Rh7DG119400
rosa_wichuraiana	Rw1G028850 Rw7G010060 Rw7G010150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	16
AccB1I	GGYRCC	2 cut(s)	814, 823
AciI	CCGC	2 cut(s)	274, 718
AclWI	GGATC	1 cut(s)	704
AcsI	RAATTY	3 cut(s)	740, 801, 904
AcuI	CTGAAG	1 cut(s)	566
AfaI	GTAC	3 cut(s)	491, 747, 989
AgsI	TTSAA	3 cut(s)	200, 249, 701
AluBI	AGCT	5 cut(s)	133, 331, 385, 432, 486
AluI	AGCT	5 cut(s)	133, 331, 385, 432, 486
Alw21I	GWGCWC	1 cut(s)	898
Alw26I	GTCTC	1 cut(s)	519
AlwI	GGATC	1 cut(s)	704
AlwNI	CAGNNNCTG	1 cut(s)	985
Ama87I	CYCGRG	1 cut(s)	899
AoxI	GGCC	1 cut(s)	338
ApoI	RAATTY	3 cut(s)	740, 801, 904
AspS9I	GGNCC	1 cut(s)	450
AsuC2I	CCSGG	1 cut(s)	714
AsuHPI	GGTGA	3 cut(s)	70, 334, 656
AvaI	CYCGRG	1 cut(s)	899
AvaII	GGWCC	1 cut(s)	450
AxyI	CCTNAGG	1 cut(s)	819
BanI	GGYRCC	2 cut(s)	814, 823
BauI	CACGAG	1 cut(s)	287
BbsI	GAAGAC	3 cut(s)	180, 447, 778
Bbv12I	GWGCWC	1 cut(s)	898
BccI	CCATC	1 cut(s)	83
BcnI	CCSGG	1 cut(s)	714
BcoDI	GTCTC	1 cut(s)	519
BfaI	CTAG	1 cut(s)	218
BfmI	CTRYAG	1 cut(s)	653
BglII	AGATCT	1 cut(s)	787
Bme1390I	CCNGG	1 cut(s)	714
Bme18I	GGWCC	1 cut(s)	450
BmeT110I	CYCGRG	1 cut(s)	899
BmgT120I	GGNCC	1 cut(s)	450
BmiI	GGNNCC	4 cut(s)	611, 620, 816, 825
BmrFI	CCNGG	1 cut(s)	714
BpiI	GAAGAC	3 cut(s)	180, 447, 778
BplI	GAGNNNNNCTC	2 cut(s)	199, 231
BpmI	CTGGAG	3 cut(s)	190, 414, 628
BpuMI	CCSGG	1 cut(s)	714
BsaJI	CCNNGG	2 cut(s)	537, 713
BsaXI	ACNNNNNCTCC	6 cut(s)	74, 80, 86, 104, 110, 116
Bse1I	ACTGG	2 cut(s)	620, 753
Bse21I	CCTNAGG	1 cut(s)	819
BseDI	CCNNGG	2 cut(s)	537, 713
BseMII	CTCAG	3 cut(s)	141, 810, 906
BseNI	ACTGG	2 cut(s)	620, 753
BseRI	GAGGAG	2 cut(s)	55, 94
BsgI	GTGCAG	1 cut(s)	381
BshFI	GGCC	1 cut(s)	340
BshNI	GGYRCC	2 cut(s)	814, 823
BsiHKAI	GWGCWC	1 cut(s)	898
BsiHKCI	CYCGRG	1 cut(s)	899
BsiSI	CCGG	1 cut(s)	713
BsmAI	GTCTC	1 cut(s)	519
BsmI	GAATGC	1 cut(s)	269
BsnI	GGCC	1 cut(s)	340
BsoBI	CYCGRG	1 cut(s)	899
Bsp1286I	GDGCHC	1 cut(s)	898
Bsp1407I	TGTACA	1 cut(s)	987
Bsp143I	GATC	2 cut(s)	709, 787
BspACI	CCGC	2 cut(s)	274, 718
BspANI	GGCC	1 cut(s)	340
BspCNI	CTCAG	3 cut(s)	140, 811, 907
BspLI	GGNNCC	4 cut(s)	611, 620, 816, 825
BspMAI	CTGCAG	1 cut(s)	657
BspPI	GGATC	1 cut(s)	704
BspT107I	GGYRCC	2 cut(s)	814, 823
BsrGI	TGTACA	1 cut(s)	987
BsrI	ACTGG	2 cut(s)	620, 753
BssECI	CCNNGG	2 cut(s)	537, 713
BssMI	GATC	2 cut(s)	709, 787
BssSI	CACGAG	1 cut(s)	287
BssT1I	CCWWGG	1 cut(s)	537
Bst2BI	CACGAG	1 cut(s)	287
Bst4CI	ACNGT	3 cut(s)	46, 473, 987
Bst6I	CTCTTC	5 cut(s)	200, 236, 312, 702, 759
BstAUI	TGTACA	1 cut(s)	987
BstDEI	CTNAG	3 cut(s)	127, 819, 915
BstKTI	GATC	2 cut(s)	712, 790
BstMAI	GTCTC	1 cut(s)	519
BstMBI	GATC	2 cut(s)	709, 787
BstMWI	GCNNNNNNNGC	7 cut(s)	273, 282, 337, 359, 823, 832, 918
BstNSI	RCATGY	1 cut(s)	179
BstSCI	CCNGG	1 cut(s)	712
BstSFI	CTRYAG	1 cut(s)	653
BstV2I	GAAGAC	3 cut(s)	180, 447, 778
BstX2I	RGATCY	1 cut(s)	787
BstYI	RGATCY	1 cut(s)	787
Bsu36I	CCTNAGG	1 cut(s)	819
BsuRI	GGCC	1 cut(s)	340
BtgZI	GCGATG	1 cut(s)	143
BtsIMutI	CAGTG	2 cut(s)	613, 912
CaiI	CAGNNNCTG	1 cut(s)	985
Cfr13I	GGNCC	1 cut(s)	450
CseI	GACGC	1 cut(s)	174
Csp6I	GTAC	3 cut(s)	490, 746, 988
CviAII	CATG	5 cut(s)	165, 176, 403, 675, 886
CviJI	RGCY	9 cut(s)	133, 331, 340, 385, 432, 486, 612, 921, 963
CviKI_1	RGCY	9 cut(s)	133, 331, 340, 385, 432, 486, 612, 921, 963
CviQI	GTAC	3 cut(s)	490, 746, 988
DdeI	CTNAG	3 cut(s)	127, 819, 915
DpnI	GATC	2 cut(s)	711, 789
DpnII	GATC	2 cut(s)	709, 787
DrdI	GACNNNNNNGTC	1 cut(s)	16
DseDI	GACNNNNNNGTC	1 cut(s)	16
Eam1104I	CTCTTC	5 cut(s)	200, 236, 312, 702, 759
EarI	CTCTTC	5 cut(s)	200, 236, 312, 702, 759
Eco130I	CCWWGG	1 cut(s)	537
Eco147I	AGGCCT	1 cut(s)	340
Eco47I	GGWCC	1 cut(s)	450
Eco57I	CTGAAG	1 cut(s)	566
Eco81I	CCTNAGG	1 cut(s)	819
Eco88I	CYCGRG	1 cut(s)	899
EcoO109I	RGGNCCY	1 cut(s)	450
EcoRI	GAATTC	1 cut(s)	801
EcoT14I	CCWWGG	1 cut(s)	537
ErhI	CCWWGG	1 cut(s)	537
FaeI	CATG	5 cut(s)	168, 179, 406, 678, 889
FalI	AAGNNNNNCTT	2 cut(s)	554, 586
FatI	CATG	5 cut(s)	164, 175, 402, 674, 885
FauI	CCCGC	1 cut(s)	281
FspBI	CTAG	1 cut(s)	218
GsuI	CTGGAG	3 cut(s)	190, 414, 628
HaeIII	GGCC	1 cut(s)	340
HapII	CCGG	1 cut(s)	713
HgaI	GACGC	1 cut(s)	174
Hin1II	CATG	5 cut(s)	168, 179, 406, 678, 889
HinfI	GANTC	7 cut(s)	62, 89, 95, 101, 389, 649, 692
HpaII	CCGG	1 cut(s)	713
HphI	GGTGA	3 cut(s)	70, 334, 656
Hpy166II	GTNNAC	1 cut(s)	990
Hpy188I	TCNGA	8 cut(s)	70, 94, 100, 109, 130, 140, 439, 661
Hpy188III	TCNNGA	4 cut(s)	218, 369, 393, 899
Hpy8I	GTNNAC	1 cut(s)	990
Hpy99I	CGWCG	1 cut(s)	50
HpyAV	CCTTC	2 cut(s)	884, 934
HpyCH4III	ACNGT	3 cut(s)	46, 473, 987
HpyCH4IV	ACGT	2 cut(s)	358, 1001
HpyCH4V	TGCA	4 cut(s)	362, 496, 655, 998
HpyF10VI	GCNNNNNNNGC	7 cut(s)	273, 282, 337, 359, 823, 832, 918
HpyF3I	CTNAG	3 cut(s)	127, 819, 915
HpySE526I	ACGT	2 cut(s)	358, 1001
Hsp92II	CATG	5 cut(s)	168, 179, 406, 678, 889
Kzo9I	GATC	2 cut(s)	709, 787
LmnI	GCTCC	2 cut(s)	609, 901
MaeI	CTAG	1 cut(s)	218
MaeII	ACGT	2 cut(s)	358, 1001
MaeIII	GTNAC	3 cut(s)	3, 407, 854
MalI	GATC	2 cut(s)	711, 789
MboI	GATC	2 cut(s)	709, 787
MflI	RGATCY	1 cut(s)	787
MhlI	GDGCHC	1 cut(s)	898
MluCI	AATT	4 cut(s)	627, 740, 801, 904
MlyI	GAGTC	6 cut(s)	56, 83, 89, 95, 383, 658
MmeI	TCCRAC	5 cut(s)	45, 117, 123, 132, 639
MseI	TTAA	6 cut(s)	228, 519, 531, 555, 626, 876
MslI	CAYNNNNRTG	2 cut(s)	642, 758
MspI	CCGG	1 cut(s)	713
MspR9I	CCNGG	1 cut(s)	714
Mva1269I	GAATGC	1 cut(s)	269
MwoI	GCNNNNNNNGC	7 cut(s)	273, 282, 337, 359, 823, 832, 918
NciI	CCSGG	1 cut(s)	714
NdeII	GATC	2 cut(s)	709, 787
NlaIII	CATG	5 cut(s)	168, 179, 406, 678, 889
NlaIV	GGNNCC	4 cut(s)	611, 620, 816, 825
NmuCI	GTSAC	1 cut(s)	3
NspI	RCATGY	1 cut(s)	179
PceI	AGGCCT	1 cut(s)	340
PctI	GAATGC	1 cut(s)	269
PfeI	GAWTC	1 cut(s)	692
PleI	GAGTC	6 cut(s)	56, 83, 89, 95, 383, 657
PpsI	GAGTC	6 cut(s)	56, 83, 89, 95, 383, 657
PpuMI	RGGWCCY	1 cut(s)	450
Psp5II	RGGWCCY	1 cut(s)	450
PspN4I	GGNNCC	4 cut(s)	611, 620, 816, 825
PspPI	GGNCC	1 cut(s)	450
PspPPI	RGGWCCY	1 cut(s)	450
PstI	CTGCAG	1 cut(s)	657
PstNI	CAGNNNCTG	1 cut(s)	985
PsuI	RGATCY	1 cut(s)	787
RsaI	GTAC	3 cut(s)	491, 747, 989
RsaNI	GTAC	3 cut(s)	490, 746, 988
RseI	CAYNNNNRTG	2 cut(s)	642, 758
SaqAI	TTAA	6 cut(s)	228, 519, 531, 555, 626, 876
Sau3AI	GATC	2 cut(s)	709, 787
Sau96I	GGNCC	1 cut(s)	450
SchI	GAGTC	6 cut(s)	56, 83, 89, 95, 383, 658
ScrFI	CCNGG	1 cut(s)	714
SduI	GDGCHC	1 cut(s)	898
SfcI	CTRYAG	1 cut(s)	653
SinI	GGWCC	1 cut(s)	450
SmiMI	CAYNNNNRTG	2 cut(s)	642, 758
Sse9I	AATT	4 cut(s)	627, 740, 801, 904
SseBI	AGGCCT	1 cut(s)	340
SsiI	CCGC	2 cut(s)	274, 718
SspMI	CTAG	1 cut(s)	218
StuI	AGGCCT	1 cut(s)	340
StyD4I	CCNGG	1 cut(s)	712
StyI	CCWWGG	1 cut(s)	537
TaaI	ACNGT	3 cut(s)	46, 473, 987
TaiI	ACGT	2 cut(s)	361, 1004
TaqI	TCGA	5 cut(s)	48, 60, 152, 191, 846
TasI	AATT	4 cut(s)	627, 740, 801, 904
TatI	WGTACW	3 cut(s)	489, 745, 987
TfiI	GAWTC	1 cut(s)	692
Tru1I	TTAA	6 cut(s)	228, 519, 531, 555, 626, 876
Tru9I	TTAA	6 cut(s)	228, 519, 531, 555, 626, 876
TscAI	CASTG	2 cut(s)	620, 919
TseFI	GTSAC	1 cut(s)	3
Tsp45I	GTSAC	1 cut(s)	3
TspDTI	ATGAA	4 cut(s)	391, 738, 874, 950
TspGWI	ACGGA	1 cut(s)	22
TspRI	CASTG	2 cut(s)	620, 919
VpaK11BI	GGWCC	1 cut(s)	450
XapI	RAATTY	3 cut(s)	740, 801, 904
XbaI	TCTAGA	1 cut(s)	217
XceI	RCATGY	1 cut(s)	179
XspI	CTAG	1 cut(s)	218

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.