MD14G1214300.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005657
Physical Location & Seq
Forward (+)
29849283 .. 29851211
1929 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1214300.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGCGGCCTTTACAGCCTCTGCAGCTCCGGCGGCTCCTGCTCCGCTACCTCTCCACCTCCTCTTCATCGCCGTCGTCTTCCCAAACACTCCAAACCCACATTCCATTTCTCCCCCAAAACGCCACCCATTTCCGTCATTCCCGACCCGCCATAGTCACCGGAACAAGTTCCCACTTTCTATCTCTACCCCATTTCTTTCTCTCTAAACCCTTCTCTTCCTCCGCCCCACCGACCCAAGATCCAGATCAGTCCGAATTAGCTCAGTCTTTCTCCTCCGAGCTCCTACAGGATTCTAGCTCCGACCCCCTCTCCGTAACCCAACGCCTCCAATTGAGCTTCTCCCACATCACCCCGACAGCTCCCCTGGTCCTTAACGTACTCAATCAGTCCCCCGAGGCCGGCCGTACGGTTTTAGGGTTTAACCAGTGGCTGATCTCGAACCCTAATTTCGAGCACACGGACGAGACCCTTTCGTATTTTGTGGATTACTTTGGGAGGAGGAAGGATTTCAAGGCGACCCACGATGTGATTGTGAGTGCAGGTGGAGTTGCTGGGGCGAAAACCTTTGCTTCCGCCATTGATAGGCTGGTGAGAGCTGGGAGGCCTGCTCAGGCTGTTTCTTTCTTTGAGAAGATGGAGAAGGATTACGGGTTGAAGCGCGACAAAGATTCGCTGAAATTGGTTATCGAAAAGCTCTGTGAAAACGGTTTTGCGAGCAATGCTGAGAAAATGGTGAAGGGTTTGGCCAATGAGTTTTTCCCTGATGAGTATATGTGTGATTTGCTGATCAAGGGCTGGTGTGTTGATGGGAAGCTCGAGGAGGCCAGGAGATTGGCTGGAGAGATGTATAGAGGAGGGTTTGAGATTGGTTCTACTGCTTACAATGCGATTCTCGATTGCGTTTCCAAGCTTTGTAGGAAGAAGGATCCCTTTCGCCTTCATTCGGAGGTGGAGGAAATCTTGGTGGATATGGACACTCATGGGGTTCCGAGAAATACAGAGGATGCTCTGAACCTGTTTGAGAGAATGGGTGAGTGGGGGTGTTACCCGAATGAAACTACATTTCTTGTGTTGATCAGGAGCTTGTATCAGGCGGCAAGGATTGGGGAAGGGGATGAAATGATTGATAGGATGAAGTCTGCCGGGTTTGGCGAGGCTCTTAATAAGAAGGAATATTATGGATTCTTAAAGATTTTGTGTGGCATTGAGAGAATCGATCACGCATTACGTGTTTTCAAGAAGATGAAGGAGGATAAATGTGAGCCCGGAATCAAGACTTATGAGCTGTTGATGGGAAAACTGTATGCTCACAACCGCGTCGATAGAGCCACTGCGCTGTTCAATGAGGCTCAAAAGAGAGGGGTGCCTTTGACACAGAAGGCGTATCCGGTGGACCTGTATTTCTTGAAGAAGAAGAAGGAGCTAAAGGCTTTGAAGAAGGGTGCGAAGAAGGAGAAGAAGCGGGAAACTTTCCCTGAGAAGATGGCAAGGAAGAAGAGACGGTTGAAGCAAATTCGATTGAGTTTCGTAAAGAAGCCGAAGAAAATGCAGCGTCGAGCCTACTGA

Protein Analysis

522

Amino Acids

59.32

Weight (kDa)

9.48

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 332 - 362 6e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 530
Acc36I ACCTGC 1 cut(s) 530
AccB1I GGYRCC 1 cut(s) 1363
AccII CGCG 2 cut(s) 660, 1317
AciI CCGC 9 cut(s) 4, 31, 43, 147, 222, 573, 1094, 1315, 1462
AclWI GGATC 3 cut(s) 233, 920, 933
AcoI YGGCCR 2 cut(s) 400, 744
AcsI RAATTY 1 cut(s) 1512
AdeI CACNNNGTG 1 cut(s) 526
AfaI GTAC 2 cut(s) 378, 406
AfiI CCNNNNNNNGG 3 cut(s) 398, 582, 943
AflIII ACRYGT 1 cut(s) 1228
AgsI TTSAA 7 cut(s) 511, 655, 1237, 1342, 1408, 1435, 1507
AjnI CCWGG 2 cut(s) 363, 824
AjuI GAANNNNNNNTTGG 4 cut(s) 84, 116, 740, 772
Alw21I GWGCWC 2 cut(s) 282, 456
Alw26I GTCTC 2 cut(s) 458, 1492
AlwI GGATC 3 cut(s) 233, 920, 933
AlwNI CAGNNNCTG 2 cut(s) 19, 430
Ama87I CYCGRG 2 cut(s) 392, 815
AoxI GGCC 6 cut(s) 5, 396, 400, 602, 744, 822
ApeKI GCWGC 2 cut(s) 22, 1549
ApoI RAATTY 1 cut(s) 1512
Asp700I GAANNNNTTC 2 cut(s) 166, 506
AspLEI GCGC 2 cut(s) 660, 1336
AspS9I GGNCC 2 cut(s) 367, 1393
AsuC2I CCSGG 2 cut(s) 1144, 1266
AsuHPI GGTGA 5 cut(s) 148, 340, 601, 745, 1043
AvaI CYCGRG 2 cut(s) 392, 815
AvaII GGWCC 2 cut(s) 367, 1393
BalI TGGCCA 1 cut(s) 746
BamHI GGATCC 1 cut(s) 925
BanI GGYRCC 1 cut(s) 1363
BanII GRGCYC 2 cut(s) 282, 1266
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 282, 456
BbvI GCAGC 2 cut(s) 34, 1561
BccI CCATC 4 cut(s) 628, 800, 1285, 1477
BceAI ACGGC 2 cut(s) 55, 387
BcgI CGANNNNNNTGC 2 cut(s) 1528, 1562
BciT130I CCWGG 2 cut(s) 365, 826
BciVI GTATCC 1 cut(s) 1395
BclI TGATCA 2 cut(s) 786, 1074
BcnI CCSGG 2 cut(s) 1144, 1266
BcoDI GTCTC 2 cut(s) 458, 1492
BfaI CTAG 1 cut(s) 294
BfmI CTRYAG 2 cut(s) 20, 284
BfuAI ACCTGC 1 cut(s) 530
BfuI GTATCC 1 cut(s) 1395
BisI GCNGC 5 cut(s) 5, 23, 32, 1095, 1550
BlsI GCNGC 5 cut(s) 6, 24, 33, 1096, 1551
Bme1390I CCNGG 4 cut(s) 365, 826, 1144, 1266
Bme18I GGWCC 2 cut(s) 367, 1393
BmeT110I CYCGRG 2 cut(s) 392, 815
BmgT120I GGNCC 2 cut(s) 367, 1393
BmiI GGNNCC 4 cut(s) 35, 927, 987, 1365
BmrFI CCNGG 4 cut(s) 365, 826, 1144, 1266
BmsI GCATC 1 cut(s) 994
BpiI GAAGAC 1 cut(s) 69
BplI GAGNNNNNCTC 2 cut(s) 592, 624
BpmI CTGGAG 1 cut(s) 858
Bpu10I CCTNAGC 1 cut(s) 609
BpuMI CCSGG 2 cut(s) 1144, 1266
Bsa29I ATCGAT 1 cut(s) 1215
BsaAI YACGTR 1 cut(s) 1229
BsaBI GATNNNNATC 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 2 cut(s) 363, 393
BsaWI WCCGGW 2 cut(s) 158, 1387
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 3 cut(s) 398, 582, 943
Bse118I RCCGGY 1 cut(s) 398
Bse1I ACTGG 1 cut(s) 424
Bse3DI GCAATG 1 cut(s) 724
Bse8I GATNNNNATC 1 cut(s) 243
BseBI CCWGG 2 cut(s) 365, 826
BseCI ATCGAT 1 cut(s) 1215
BseDI CCNNGG 2 cut(s) 363, 393
BseGI GGATG 3 cut(s) 1009, 1120, 1137
BseJI GATNNNNATC 1 cut(s) 243
BseLI CCNNNNNNNGG 3 cut(s) 398, 582, 943
BseMI GCAATG 1 cut(s) 724
BseMII CTCAG 4 cut(s) 275, 623, 714, 1467
BseNI ACTGG 1 cut(s) 424
BseRI GAGGAG 5 cut(s) 49, 262, 511, 833, 867
BseX3I CGGCCG 1 cut(s) 400
BseXI GCAGC 2 cut(s) 34, 1561
BseYI CCCAGC 2 cut(s) 551, 596
BsgI GTGCAG 1 cut(s) 558
Bsh1236I CGCG 2 cut(s) 660, 1317
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 6 cut(s) 7, 398, 402, 604, 746, 824
BshNI GGYRCC 1 cut(s) 1363
BshVI ATCGAT 1 cut(s) 1215
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 2 cut(s) 282, 456
BsiHKCI CYCGRG 2 cut(s) 392, 815
BsiSI CCGG 6 cut(s) 28, 159, 399, 1143, 1266, 1388
BsiWI CGTACG 1 cut(s) 404
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 3 cut(s) 398, 582, 943
BsmAI GTCTC 2 cut(s) 458, 1492
BsmBI CGTCTC 1 cut(s) 1492
BsmFI GGGAC 1 cut(s) 373
BsnI GGCC 6 cut(s) 7, 398, 402, 604, 746, 824
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 2 cut(s) 392, 815
Bsp1286I GDGCHC 3 cut(s) 282, 456, 1266
Bsp143I GATC 7 cut(s) 238, 244, 432, 786, 925, 1074, 1216
BspACI CCGC 9 cut(s) 4, 31, 43, 147, 222, 573, 1094, 1315, 1462
BspANI GGCC 6 cut(s) 7, 398, 402, 604, 746, 824
BspCNI CTCAG 4 cut(s) 274, 622, 715, 1468
BspDI ATCGAT 1 cut(s) 1215
BspFNI CGCG 2 cut(s) 660, 1317
BspLI GGNNCC 4 cut(s) 35, 927, 987, 1365
BspMAI CTGCAG 1 cut(s) 24
BspMI ACCTGC 1 cut(s) 530
BspPI GGATC 3 cut(s) 233, 920, 933
BspT107I GGYRCC 1 cut(s) 1363
BspTNI GGTCTC 1 cut(s) 458
BsrDI GCAATG 1 cut(s) 724
BsrFI RCCGGY 1 cut(s) 398
BsrI ACTGG 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 2 cut(s) 363, 393
BssMI GATC 7 cut(s) 238, 244, 432, 786, 925, 1074, 1216
Bst2UI CCWGG 2 cut(s) 365, 826
Bst4CI ACNGT 4 cut(s) 409, 707, 1302, 1503
Bst6I CTCTTC 3 cut(s) 67, 220, 1490
BstBAI YACGTR 1 cut(s) 1229
BstC8I GCNNGC 3 cut(s) 400, 606, 715
BstDEI CTNAG 4 cut(s) 261, 609, 723, 1476
BstF5I GGATG 3 cut(s) 1009, 1120, 1137
BstFNI CGCG 2 cut(s) 660, 1317
BstHHI GCGC 2 cut(s) 660, 1336
BstKTI GATC 7 cut(s) 241, 247, 435, 789, 928, 1077, 1219
BstMAI GTCTC 2 cut(s) 458, 1492
BstMBI GATC 7 cut(s) 238, 244, 432, 786, 925, 1074, 1216
BstMCI CGRYCG 1 cut(s) 403
BstMWI GCNNNNNNNGC 7 cut(s) 13, 22, 28, 31, 37, 719, 884
BstNI CCWGG 2 cut(s) 365, 826
BstSCI CCNGG 4 cut(s) 363, 824, 1142, 1264
BstSFI CTRYAG 2 cut(s) 20, 284
BstUI CGCG 2 cut(s) 660, 1317
BstV1I GCAGC 2 cut(s) 34, 1561
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 2 cut(s) 238, 925
BstXI CCANNNNNNTGG 1 cut(s) 832
BstYI RGATCY 2 cut(s) 238, 925
BstZI CGGCCG 1 cut(s) 400
Bsu15I ATCGAT 1 cut(s) 1215
BsuI GTATCC 1 cut(s) 1395
BsuRI GGCC 6 cut(s) 7, 398, 402, 604, 746, 824
BsuTUI ATCGAT 1 cut(s) 1215
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 3 cut(s) 1009, 1120, 1137
BtsI GCAGTG 1 cut(s) 1329
BtsIMutI CAGTG 2 cut(s) 431, 1329
BveI ACCTGC 1 cut(s) 530
Cac8I GCNNGC 3 cut(s) 400, 606, 715
CaiI CAGNNNCTG 2 cut(s) 19, 430
CfoI GCGC 2 cut(s) 660, 1336
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 2 cut(s) 367, 1393
ClaI ATCGAT 1 cut(s) 1215
CseI GACGC 2 cut(s) 1306, 1541
Csp6I GTAC 2 cut(s) 377, 405
CviAII CATG 1 cut(s) 980
CviQI GTAC 2 cut(s) 377, 405
DdeI CTNAG 4 cut(s) 261, 609, 723, 1476
DpnI GATC 7 cut(s) 240, 246, 434, 788, 927, 1076, 1218
DpnII GATC 7 cut(s) 238, 244, 432, 786, 925, 1074, 1216
DraIII CACNNNGTG 1 cut(s) 526
EaeI YGGCCR 2 cut(s) 400, 744
EagI CGGCCG 1 cut(s) 400
Eam1104I CTCTTC 3 cut(s) 67, 220, 1490
EarI CTCTTC 3 cut(s) 67, 220, 1490
EciI GGCGGA 2 cut(s) 211, 562
Ecl136II GAGCTC 1 cut(s) 280
EclXI CGGCCG 1 cut(s) 400
Eco147I AGGCCT 1 cut(s) 604
Eco24I GRGCYC 2 cut(s) 282, 1266
Eco31I GGTCTC 1 cut(s) 458
Eco47I GGWCC 2 cut(s) 367, 1393
Eco52I CGGCCG 1 cut(s) 400
Eco53kI GAGCTC 1 cut(s) 280
Eco88I CYCGRG 2 cut(s) 392, 815
EcoICRI GAGCTC 1 cut(s) 280
EcoRII CCWGG 2 cut(s) 363, 824
EcoT38I GRGCYC 2 cut(s) 282, 1266
Esp3I CGTCTC 1 cut(s) 1492
FaeI CATG 1 cut(s) 983
FaiI YATR 9 cut(s) 152, 771, 773, 849, 971, 981, 1179, 1281, 1305
FalI AAGNNNNNCTT 2 cut(s) 914, 946
FaqI GGGAC 1 cut(s) 373
FatI CATG 1 cut(s) 979
FauI CCCGC 2 cut(s) 154, 1455
FbaI TGATCA 2 cut(s) 786, 1074
Fnu4HI GCNGC 5 cut(s) 5, 23, 32, 1095, 1550
FokI GGATG 3 cut(s) 1016, 1127, 1144
FriOI GRGCYC 2 cut(s) 282, 1266
FseI GGCCGGCC 1 cut(s) 402
Fsp4HI GCNGC 5 cut(s) 5, 23, 32, 1095, 1550
FspBI CTAG 1 cut(s) 294
GlaI GCGC 2 cut(s) 659, 1335
GluI GCNGC 5 cut(s) 5, 23, 32, 1095, 1550
GsaI CCCAGC 2 cut(s) 555, 600
GsuI CTGGAG 1 cut(s) 858
HaeIII GGCC 6 cut(s) 7, 398, 402, 604, 746, 824
HapII CCGG 6 cut(s) 28, 159, 399, 1143, 1266, 1388
HgaI GACGC 2 cut(s) 1306, 1541
HhaI GCGC 2 cut(s) 660, 1336
Hin1II CATG 1 cut(s) 983
Hin6I GCGC 2 cut(s) 658, 1334
HinP1I GCGC 2 cut(s) 658, 1334
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 6 cut(s) 290, 668, 889, 1182, 1212, 1269
HpaII CCGG 6 cut(s) 28, 159, 399, 1143, 1266, 1388
HphI GGTGA 5 cut(s) 148, 340, 601, 745, 1043
Hpy166II GTNNAC 1 cut(s) 1393
Hpy188I TCNGA 6 cut(s) 253, 277, 301, 946, 990, 1011
Hpy188III TCNNGA 8 cut(s) 141, 242, 436, 893, 1078, 1237, 1273, 1405
Hpy8I GTNNAC 1 cut(s) 1393
Hpy99I CGWCG 3 cut(s) 76, 1322, 1557
HpyCH4III ACNGT 4 cut(s) 409, 707, 1302, 1503
HpyCH4IV ACGT 2 cut(s) 375, 1228
HpyCH4V TGCA 3 cut(s) 22, 539, 1549
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 22, 28, 31, 37, 719, 884
HpyF3I CTNAG 4 cut(s) 261, 609, 723, 1476
HpySE526I ACGT 2 cut(s) 375, 1228
Hsp92II CATG 1 cut(s) 983
HspAI GCGC 2 cut(s) 658, 1334
KroI GCCGGC 1 cut(s) 398
KroNI GCCGGC 1 cut(s) 400
Ksp22I TGATCA 2 cut(s) 786, 1074
Kzo9I GATC 7 cut(s) 238, 244, 432, 786, 925, 1074, 1216
LmnI GCTCC 8 cut(s) 30, 39, 45, 285, 302, 364, 1080, 1420
Lsp1109I GCAGC 2 cut(s) 34, 1561
LweI GCATC 1 cut(s) 994
MaeI CTAG 1 cut(s) 294
MaeII ACGT 2 cut(s) 375, 1228
MaeIII GTNAC 3 cut(s) 154, 313, 1043
MalI GATC 7 cut(s) 240, 246, 434, 788, 927, 1076, 1218
MboI GATC 7 cut(s) 238, 244, 432, 786, 925, 1074, 1216
MfeI CAATTG 1 cut(s) 329
MflI RGATCY 2 cut(s) 238, 925
MhlI GDGCHC 3 cut(s) 282, 456, 1266
MlsI TGGCCA 1 cut(s) 746
MluCI AATT 5 cut(s) 254, 329, 445, 677, 1512
MluNI TGGCCA 1 cut(s) 746
MmeI TCCRAC 1 cut(s) 324
Mox20I TGGCCA 1 cut(s) 746
MroNI GCCGGC 1 cut(s) 398
MroXI GAANNNNTTC 2 cut(s) 166, 506
MscI TGGCCA 1 cut(s) 746
MseI TTAA 4 cut(s) 372, 420, 1161, 1187
Msp20I TGGCCA 1 cut(s) 746
MspI CCGG 6 cut(s) 28, 159, 399, 1143, 1266, 1388
MspR9I CCNGG 4 cut(s) 365, 826, 1144, 1266
MunI CAATTG 1 cut(s) 329
MvaI CCWGG 2 cut(s) 365, 826
MvnI CGCG 2 cut(s) 660, 1317
MwoI GCNNNNNNNGC 7 cut(s) 13, 22, 28, 31, 37, 719, 884
NaeI GCCGGC 1 cut(s) 400
NciI CCSGG 2 cut(s) 1144, 1266
NdeII GATC 7 cut(s) 238, 244, 432, 786, 925, 1074, 1216
NgoMIV GCCGGC 1 cut(s) 398
NlaIII CATG 1 cut(s) 983
NlaIV GGNNCC 4 cut(s) 35, 927, 987, 1365
NmuCI GTSAC 1 cut(s) 154
PaeR7I CTCGAG 1 cut(s) 815
PaqCI CACCTGC 1 cut(s) 530
PceI AGGCCT 1 cut(s) 604
PdiI GCCGGC 1 cut(s) 400
PdmI GAANNNNTTC 2 cut(s) 166, 506
PfeI GAWTC 6 cut(s) 290, 668, 889, 1182, 1212, 1269
Pfl23II CGTACG 1 cut(s) 404
PkrI GCNGC 5 cut(s) 6, 24, 33, 1096, 1551
Ppu21I YACGTR 1 cut(s) 1229
Psp124BI GAGCTC 1 cut(s) 282
Psp6I CCWGG 2 cut(s) 363, 824
PspFI CCCAGC 2 cut(s) 551, 596
PspGI CCWGG 2 cut(s) 363, 824
PspLI CGTACG 1 cut(s) 404
PspN4I GGNNCC 4 cut(s) 35, 927, 987, 1365
PspPI GGNCC 2 cut(s) 367, 1393
PspXI VCTCGAGB 1 cut(s) 815
PstI CTGCAG 1 cut(s) 24
PstNI CAGNNNCTG 2 cut(s) 19, 430
PsuI RGATCY 2 cut(s) 238, 925
RigI GGCCGGCC 1 cut(s) 402
RsaI GTAC 2 cut(s) 378, 406
RsaNI GTAC 2 cut(s) 377, 405
SacI GAGCTC 1 cut(s) 282
SaqAI TTAA 4 cut(s) 372, 420, 1161, 1187
SatI GCNGC 5 cut(s) 5, 23, 32, 1095, 1550
Sau3AI GATC 7 cut(s) 238, 244, 432, 786, 925, 1074, 1216
Sau96I GGNCC 2 cut(s) 367, 1393
ScrFI CCNGG 4 cut(s) 365, 826, 1144, 1266
SduI GDGCHC 3 cut(s) 282, 456, 1266
SfaNI GCATC 1 cut(s) 994
SfcI CTRYAG 2 cut(s) 20, 284
Sfr274I CTCGAG 1 cut(s) 815
SinI GGWCC 2 cut(s) 367, 1393
SlaI CTCGAG 1 cut(s) 815
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
Sse9I AATT 5 cut(s) 254, 329, 445, 677, 1512
SseBI AGGCCT 1 cut(s) 604
SsiI CCGC 9 cut(s) 4, 31, 43, 147, 222, 573, 1094, 1315, 1462
SspI AATATT 1 cut(s) 1175
SspMI CTAG 1 cut(s) 294
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 1 cut(s) 604
StyD4I CCNGG 4 cut(s) 363, 824, 1142, 1264
TaaI ACNGT 4 cut(s) 409, 707, 1302, 1503
TaiI ACGT 2 cut(s) 378, 1231
TaqI TCGA 9 cut(s) 437, 450, 687, 816, 894, 1215, 1320, 1516, 1555
TasI AATT 5 cut(s) 254, 329, 445, 677, 1512
TauI GCSGC 3 cut(s) 7, 34, 1097
TfiI GAWTC 6 cut(s) 290, 668, 889, 1182, 1212, 1269
Tru1I TTAA 4 cut(s) 372, 420, 1161, 1187
Tru9I TTAA 4 cut(s) 372, 420, 1161, 1187
TscAI CASTG 2 cut(s) 431, 1336
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 2 cut(s) 22, 1549
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 6 cut(s) 54, 929, 1068, 1131, 1148, 1259
TspGWI ACGGA 3 cut(s) 122, 301, 473
TspRI CASTG 2 cut(s) 431, 1336
VpaK11BI GGWCC 2 cut(s) 367, 1393
XapI RAATTY 1 cut(s) 1512
XcmI CCANNNNNNNNNTGG 1 cut(s) 583
XhoI CTCGAG 1 cut(s) 815
XmnI GAANNNNTTC 2 cut(s) 166, 506
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.