MD14G1246700.v1.1

Protein of unknown function (DUF1308)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007299
Physical Location & Seq
Forward (+)
32434609 .. 32438899
4291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1246700.v1.1.491

Sequence Viewer

Length: 1521 bp
ATGGAAGTCGGAAGAAAGGAAGTGATTGATGGAGCAGAAGCAGATGCATATGCAGCACAACAAGCAGCAGTGGAATTGGAATTGGCAAGGAAGAGATGCAGATTTGTGATAGGGCGGATTGAGGGATTGCCAGCTCCTACCAACATCAAACGCACTCTTCTCAAACTGGTTGACTCCGAACTCATTTTTCTCTCCCGCTCCTCCTCCGCCGCCTCCTCCTCCACTTCTTCAACTCCCCTCACCAACATCAAGAGTGTCAATATCGGACACATCGAGGCAGTTGTTCACATTCTCCGGCAGCCCTTCATTACTGGGGTTTCTCGCGTTTGCAAGCCAATCCCTTTCTCAACCTTAGCCCCAGTTGCACAGCAAACCGACCCTTGTGTCAACCACAAACACGTCCATGTTGATATAGTTTGCACACTCCATGGGAACCCTGTCTGGATTGTTGTCTCCGACAGGAACCCTAAATACATTACTTGGACTGGCTTCCGATCCCCTTCCCATCATAAGAGTAGGGGCTTAAAATTGCGTATTCAGCAACTCACTGCTGCTGCCCGCTCTGCTGTGGCACTCAAACCTTCCTCGATTATTCTTTTCTTCTCATACCGAAACGGTCTTAGCAGCGTTATTTGTGATAGACTAAAAGAGGAGTTTGGGGCAACTGAGTTTCGATTGGATTTTCCTATTTTAGACTTTGATTTTGATTTATCCGAGGAACCTGGTGACTGGACTAATGTACTTGCCGCAAGAACGTTTCAACAGGCTTGTGTATTTGAAATAAAGGTTAATGATCCAAGGGACACTGTTTTCAGTTCAGAATCTGATGTCAAGGACTCATGCCTGGGAGAGGTGGCTGCTGAGACTTACCAGGATCCGTCTGTCTCAAAGGAGGACACTCAATTTTTCCCTGTTTTCTCTTCTCTGATTTCAAGAATGGGAATTCACTCATTGGATGCAAAAAATGTGGAATCTGTGAAACTGGGGCGTATTCTGGGTGATGGTGAACTTATTAACTTTGATACAACTGCTTTGATTGCTCTTATATCGGGTATTAGTAATGGTGGTACAGACAAGCTTTTGGCTACTCCAGAAAGTGAATTGAGGCAGCGGTTTAAGGGTAACTACGAGTTTGTGATTCAACAGGTGATGTCTGAGATTCAGAATCCAATTCTTGTTGAACTTGGTCGCGCAATGTCTGGAAAGAGAGGCATAATCTGTGAAAGTGTCCTTTTGGAGTTCAAAGAGTTAGCATTTATGTGTGGTGGGCCCAATGAGAACTTGAGAGCTAGCCATTTGCTAAAGTATCTTACGGTTGTTCCTGATAGTCCCTCTGAACGCATGATGAGCCTTCCAACCACTAGAAAACTGGCTTTGAAGAATAAGGTTGTTTTTGGTACTGGTGATTCCTGGTGTGCTCCAACTGTAACGGCAAATATGGCATTTGTTAGAGCAATTTCACAAACTGGGATGTCCTTGTTTACGATTGAGCACAGACCGCGGGCTTTGACTGGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1308

Protein Analysis

507

Amino Acids

55.72

Weight (kDa)

7.15

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1308 PF07000 338 - 504 6.6e-28 Protein of unknown function (DUF1308)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 383
AccBSI CCGCTC 2 cut(s) 198, 561
AccII CGCG 3 cut(s) 324, 1191, 1501
AciI CCGC 9 cut(s) 115, 196, 207, 210, 559, 747, 1111, 1499, 1501
AclI AACGTT 1 cut(s) 755
AclWI GGATC 4 cut(s) 489, 788, 869, 882
AcsI RAATTY 1 cut(s) 942
AfaI GTAC 3 cut(s) 741, 1069, 1399
AfiI CCNNNNNNNGG 1 cut(s) 850
AflIII ACRYGT 1 cut(s) 397
AgsI TTSAA 8 cut(s) 231, 761, 779, 933, 1142, 1181, 1243, 1378
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 4 cut(s) 721, 843, 870, 1409
AluBI AGCT 3 cut(s) 134, 1078, 1289
AluI AGCT 3 cut(s) 134, 1078, 1289
Alw21I GWGCWC 2 cut(s) 1420, 1494
Alw26I GTCTC 3 cut(s) 457, 857, 889
AlwI GGATC 4 cut(s) 489, 788, 869, 882
AlwNI CAGNNNCTG 1 cut(s) 824
AoxI GGCC 1 cut(s) 1268
ApaI GGGCCC 1 cut(s) 1272
ApeKI GCWGC 8 cut(s) 53, 65, 298, 551, 554, 624, 857, 1108
ApoI RAATTY 1 cut(s) 942
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
AspLEI GCGC 1 cut(s) 1193
AspS9I GGNCC 2 cut(s) 1268, 1269
AsuHPI GGTGA 6 cut(s) 232, 737, 1010, 1016, 1159, 1415
AsuNHI GCTAGC 1 cut(s) 1289
BaeGI GKGCMC 1 cut(s) 1272
BamHI GGATCC 1 cut(s) 874
BanII GRGCYC 1 cut(s) 1272
Bbv12I GWGCWC 2 cut(s) 1420, 1494
BbvI GCAGC 8 cut(s) 65, 77, 310, 538, 541, 636, 844, 1120
BccI CCATC 3 cut(s) 23, 513, 995
BceAI ACGGC 1 cut(s) 1446
BciT130I CCWGG 4 cut(s) 723, 845, 872, 1411
BcoDI GTCTC 3 cut(s) 457, 857, 889
BfaI CTAG 2 cut(s) 1290, 1362
Bme1390I CCNGG 4 cut(s) 723, 845, 872, 1411
BmgBI CACGTC 1 cut(s) 400
BmgT120I GGNCC 2 cut(s) 1268, 1269
BmiI GGNNCC 5 cut(s) 434, 464, 720, 876, 1270
BmrFI CCNGG 4 cut(s) 723, 845, 872, 1411
BmrI ACTGGG 4 cut(s) 321, 353, 992, 1476
BmsI GCATC 3 cut(s) 34, 86, 946
BmtI GCTAGC 1 cut(s) 1293
BmuI ACTGGG 4 cut(s) 321, 353, 992, 1476
BpmI CTGGAG 1 cut(s) 1074
Bpu10I CCTNAGC 1 cut(s) 352
BpuEI CTTGAG 1 cut(s) 1303
BsaJI CCNNGG 5 cut(s) 427, 714, 797, 844, 1499
Bsc4I CCNNNNNNNGG 1 cut(s) 850
Bse3DI GCAATG 1 cut(s) 1200
BseBI CCWGG 4 cut(s) 723, 845, 872, 1411
BseDI CCNNGG 5 cut(s) 427, 714, 797, 844, 1499
BseGI GGATG 2 cut(s) 961, 1476
BseLI CCNNNNNNNGG 1 cut(s) 850
BseMI GCAATG 1 cut(s) 1200
BseMII CTCAG 3 cut(s) 657, 852, 1146
BseRI GAGGAG 5 cut(s) 190, 193, 205, 208, 665
BseSI GKGCMC 1 cut(s) 1272
BseXI GCAGC 8 cut(s) 65, 77, 310, 538, 541, 636, 844, 1120
Bsh1236I CGCG 3 cut(s) 324, 1191, 1501
BshFI GGCC 1 cut(s) 1270
BsiHKAI GWGCWC 2 cut(s) 1420, 1494
BsiSI CCGG 1 cut(s) 295
BslFI GGGAC 2 cut(s) 815, 1314
BslI CCNNNNNNNGG 1 cut(s) 850
BsmAI GTCTC 3 cut(s) 457, 857, 889
BsmFI GGGAC 2 cut(s) 815, 1314
BsnI GGCC 1 cut(s) 1270
Bsp120I GGGCCC 1 cut(s) 1268
Bsp1286I GDGCHC 3 cut(s) 1272, 1420, 1494
Bsp143I GATC 3 cut(s) 494, 793, 874
Bsp19I CCATGG 1 cut(s) 427
BspACI CCGC 9 cut(s) 115, 196, 207, 210, 559, 747, 1111, 1499, 1501
BspANI GGCC 1 cut(s) 1270
BspCNI CTCAG 3 cut(s) 658, 853, 1147
BspFNI CGCG 3 cut(s) 324, 1191, 1501
BspLI GGNNCC 5 cut(s) 434, 464, 720, 876, 1270
BspOI GCTAGC 1 cut(s) 1293
BspPI GGATC 4 cut(s) 489, 788, 869, 882
BsrBI CCGCTC 2 cut(s) 198, 561
BsrDI GCAATG 1 cut(s) 1200
BssECI CCNNGG 5 cut(s) 427, 714, 797, 844, 1499
BssMI GATC 3 cut(s) 494, 793, 874
BssT1I CCWWGG 2 cut(s) 427, 797
Bst2UI CCWGG 4 cut(s) 723, 845, 872, 1411
Bst4CI ACNGT 4 cut(s) 617, 808, 1315, 1426
Bst6I CTCTTC 3 cut(s) 86, 162, 925
BstC8I GCNNGC 5 cut(s) 132, 332, 559, 1291, 1503
BstDEI CTNAG 5 cut(s) 352, 620, 666, 861, 1155
BstDSI CCRYGG 2 cut(s) 427, 1499
BstENI CCTNNNNNAGG 1 cut(s) 848
BstF5I GGATG 2 cut(s) 961, 1476
BstFNI CGCG 3 cut(s) 324, 1191, 1501
BstHHI GCGC 1 cut(s) 1193
BstKTI GATC 3 cut(s) 497, 796, 877
BstMAI GTCTC 3 cut(s) 457, 857, 889
BstMBI GATC 3 cut(s) 494, 793, 874
BstMWI GCNNNNNNNGC 9 cut(s) 53, 62, 362, 538, 563, 1037, 1347, 1439, 1498
BstNI CCWGG 4 cut(s) 723, 845, 872, 1411
BstSCI CCNGG 4 cut(s) 721, 843, 870, 1409
BstSLI GKGCMC 1 cut(s) 1272
BstUI CGCG 3 cut(s) 324, 1191, 1501
BstV1I GCAGC 8 cut(s) 65, 77, 310, 538, 541, 636, 844, 1120
BstX2I RGATCY 1 cut(s) 874
BstYI RGATCY 1 cut(s) 874
BsuRI GGCC 1 cut(s) 1270
BtgI CCRYGG 2 cut(s) 427, 1499
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 2 cut(s) 961, 1476
BtsI GCAGTG 2 cut(s) 75, 546
BtsIMutI CAGTG 3 cut(s) 75, 546, 804
Cac8I GCNNGC 5 cut(s) 132, 332, 559, 1291, 1503
CaiI CAGNNNCTG 1 cut(s) 824
CfoI GCGC 1 cut(s) 1193
Cfr13I GGNCC 2 cut(s) 1268, 1269
Cfr42I CCGCGG 1 cut(s) 1502
CsiI ACCWGGT 1 cut(s) 721
Csp6I GTAC 3 cut(s) 740, 1068, 1398
CspCI CAANNNNNGTGG 2 cut(s) 948, 983
CviAII CATG 4 cut(s) 404, 428, 840, 1342
CviQI GTAC 3 cut(s) 740, 1068, 1398
DdeI CTNAG 5 cut(s) 352, 620, 666, 861, 1155
DpnI GATC 3 cut(s) 496, 795, 876
DpnII GATC 3 cut(s) 494, 793, 874
DrdI GACNNNNNNGTC 1 cut(s) 383
DseDI GACNNNNNNGTC 1 cut(s) 383
Eam1104I CTCTTC 3 cut(s) 86, 162, 925
EarI CTCTTC 3 cut(s) 86, 162, 925
EciI GGCGGA 2 cut(s) 130, 196
Eco130I CCWWGG 2 cut(s) 427, 797
Eco24I GRGCYC 1 cut(s) 1272
EcoNI CCTNNNNNAGG 1 cut(s) 848
EcoRI GAATTC 1 cut(s) 942
EcoRII CCWGG 4 cut(s) 721, 843, 870, 1409
EcoT14I CCWWGG 2 cut(s) 427, 797
EcoT22I ATGCAT 1 cut(s) 49
EcoT38I GRGCYC 1 cut(s) 1272
ErhI CCWWGG 2 cut(s) 427, 797
FaeI CATG 4 cut(s) 407, 431, 843, 1345
FaqI GGGAC 2 cut(s) 815, 1314
FatI CATG 4 cut(s) 403, 427, 839, 1341
FauI CCCGC 3 cut(s) 203, 566, 1494
FauNDI CATATG 1 cut(s) 49
FokI GGATG 2 cut(s) 968, 1483
FriOI GRGCYC 1 cut(s) 1272
FspBI CTAG 2 cut(s) 1290, 1362
GlaI GCGC 1 cut(s) 1192
GsuI CTGGAG 1 cut(s) 1074
HaeIII GGCC 1 cut(s) 1270
HapII CCGG 1 cut(s) 295
HhaI GCGC 1 cut(s) 1193
Hin1II CATG 4 cut(s) 407, 431, 843, 1345
Hin6I GCGC 1 cut(s) 1191
HinP1I GCGC 1 cut(s) 1191
HincII GTYRAC 2 cut(s) 172, 388
HindII GTYRAC 2 cut(s) 172, 388
HindIII AAGCTT 1 cut(s) 1076
HinfI GANTC 8 cut(s) 173, 821, 836, 971, 1138, 1159, 1165, 1406
HpaII CCGG 1 cut(s) 295
HphI GGTGA 6 cut(s) 232, 737, 1010, 1016, 1159, 1415
Hpy166II GTNNAC 5 cut(s) 172, 286, 388, 1007, 1482
Hpy188III TCNNGA 6 cut(s) 250, 442, 933, 1091, 1200, 1322
Hpy8I GTNNAC 5 cut(s) 172, 286, 388, 1007, 1482
HpyAV CCTTC 4 cut(s) 313, 510, 591, 1361
HpyCH4III ACNGT 4 cut(s) 617, 808, 1315, 1426
HpyCH4IV ACGT 2 cut(s) 399, 755
HpyCH4V TGCA 7 cut(s) 47, 53, 99, 330, 365, 420, 959
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 62, 362, 538, 563, 1037, 1347, 1439, 1498
HpyF3I CTNAG 5 cut(s) 352, 620, 666, 861, 1155
HpySE526I ACGT 2 cut(s) 399, 755
Hsp92II CATG 4 cut(s) 407, 431, 843, 1345
HspAI GCGC 1 cut(s) 1191
KspI CCGCGG 1 cut(s) 1502
Kzo9I GATC 3 cut(s) 494, 793, 874
LmnI GCTCC 4 cut(s) 32, 139, 203, 1423
Lsp1109I GCAGC 8 cut(s) 65, 77, 310, 538, 541, 636, 844, 1120
LweI GCATC 3 cut(s) 34, 86, 946
MabI ACCWGGT 1 cut(s) 721
MaeI CTAG 2 cut(s) 1290, 1362
MaeII ACGT 2 cut(s) 399, 755
MaeIII GTNAC 3 cut(s) 725, 1121, 1426
MalI GATC 3 cut(s) 496, 795, 876
MbiI CCGCTC 2 cut(s) 198, 561
MboI GATC 3 cut(s) 494, 793, 874
MboII GAAGA 7 cut(s) 24, 103, 149, 219, 592, 912, 1390
MflI RGATCY 1 cut(s) 874
MhlI GDGCHC 3 cut(s) 1272, 1420, 1494
MluCI AATT 8 cut(s) 74, 80, 527, 902, 942, 1100, 1170, 1455
MlyI GAGTC 2 cut(s) 167, 830
MmeI TCCRAC 3 cut(s) 480, 1379, 1445
Mph1103I ATGCAT 1 cut(s) 49
MseI TTAA 4 cut(s) 524, 789, 1014, 1116
MslI CAYNNNNRTG 2 cut(s) 402, 1258
MspA1I CMGCKG 2 cut(s) 1111, 1501
MspI CCGG 1 cut(s) 295
MspR9I CCNGG 4 cut(s) 723, 845, 872, 1411
MvaI CCWGG 4 cut(s) 723, 845, 872, 1411
MvnI CGCG 3 cut(s) 324, 1191, 1501
MwoI GCNNNNNNNGC 9 cut(s) 53, 62, 362, 538, 563, 1037, 1347, 1439, 1498
NcoI CCATGG 1 cut(s) 427
NdeI CATATG 1 cut(s) 49
NdeII GATC 3 cut(s) 494, 793, 874
NheI GCTAGC 1 cut(s) 1289
NlaIII CATG 4 cut(s) 407, 431, 843, 1345
NlaIV GGNNCC 5 cut(s) 434, 464, 720, 876, 1270
NmuCI GTSAC 1 cut(s) 725
NsiI ATGCAT 1 cut(s) 49
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PfeI GAWTC 6 cut(s) 821, 971, 1138, 1159, 1165, 1406
PleI GAGTC 2 cut(s) 167, 830
PpsI GAGTC 2 cut(s) 167, 830
Psp1406I AACGTT 1 cut(s) 755
Psp6I CCWGG 4 cut(s) 721, 843, 870, 1409
PspGI CCWGG 4 cut(s) 721, 843, 870, 1409
PspN4I GGNNCC 5 cut(s) 434, 464, 720, 876, 1270
PspOMI GGGCCC 1 cut(s) 1268
PspPI GGNCC 2 cut(s) 1268, 1269
PsrI GAACNNNNNNTAC 2 cut(s) 455, 487
PstNI CAGNNNCTG 1 cut(s) 824
PsuI RGATCY 1 cut(s) 874
RsaI GTAC 3 cut(s) 741, 1069, 1399
RsaNI GTAC 3 cut(s) 740, 1068, 1398
RseI CAYNNNNRTG 2 cut(s) 402, 1258
SacII CCGCGG 1 cut(s) 1502
SaqAI TTAA 4 cut(s) 524, 789, 1014, 1116
Sau3AI GATC 3 cut(s) 494, 793, 874
Sau96I GGNCC 2 cut(s) 1268, 1269
SchI GAGTC 2 cut(s) 167, 830
ScrFI CCNGG 4 cut(s) 723, 845, 872, 1411
SduI GDGCHC 3 cut(s) 1272, 1420, 1494
SexAI ACCWGGT 1 cut(s) 721
SfaNI GCATC 3 cut(s) 34, 86, 946
Sfr303I CCGCGG 1 cut(s) 1502
SgrBI CCGCGG 1 cut(s) 1502
SmiMI CAYNNNNRTG 2 cut(s) 402, 1258
SmlI CTYRAG 1 cut(s) 1282
SmoI CTYRAG 1 cut(s) 1282
Sse9I AATT 8 cut(s) 74, 80, 527, 902, 942, 1100, 1170, 1455
SsiI CCGC 9 cut(s) 115, 196, 207, 210, 559, 747, 1111, 1499, 1501
SspMI CTAG 2 cut(s) 1290, 1362
StyD4I CCNGG 4 cut(s) 721, 843, 870, 1409
StyI CCWWGG 2 cut(s) 427, 797
TaaI ACNGT 4 cut(s) 617, 808, 1315, 1426
TaiI ACGT 2 cut(s) 402, 758
TaqI TCGA 3 cut(s) 273, 587, 673
TasI AATT 8 cut(s) 74, 80, 527, 902, 942, 1100, 1170, 1455
TatI WGTACW 1 cut(s) 739
TauI GCSGC 2 cut(s) 212, 749
TfiI GAWTC 6 cut(s) 821, 971, 1138, 1159, 1165, 1406
Tru1I TTAA 4 cut(s) 524, 789, 1014, 1116
Tru9I TTAA 4 cut(s) 524, 789, 1014, 1116
TscAI CASTG 3 cut(s) 75, 553, 811
TseFI GTSAC 1 cut(s) 725
TseI GCWGC 8 cut(s) 53, 65, 298, 551, 554, 624, 857, 1108
Tsp45I GTSAC 1 cut(s) 725
TspDTI ATGAA 1 cut(s) 295
TspGWI ACGGA 1 cut(s) 867
TspRI CASTG 3 cut(s) 75, 553, 811
XagI CCTNNNNNAGG 1 cut(s) 848
XapI RAATTY 1 cut(s) 942
XcmI CCANNNNNNNNNTGG 1 cut(s) 1366
XspI CTAG 2 cut(s) 1290, 1362
Zsp2I ATGCAT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.