Rorug06G0402200

Protein of unknown function (DUF1308)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007299
Physical Location & Seq
Forward (+)
54679293 .. 54681532
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0402200.1

Sequence Viewer

Length: 1581 bp
ATGGCCAGTAGTACTGAATTAGGTGATCATGATCATGATGAATACTTGATCAGTAAAGTTTCAATCACCGAAACCGTACTGGTGTCTTCTGCACCAGTTTTGCAGCAGCCTCCAAAAGTTGTCAGCCTCTCAAACTTGGACCGCCAGTGTCCACTTCTTATGTACTTGGTTTTCTTTTACAAATATCCCACCACCTCCTCTGTTGATCATAATCGAATTTGGTCGCTTGATTCAGTTTTTCAAAGCTTGAAGTCAGGCTTGGAAGAGACTTTATCCATTTGGTACCCAGCGGGAGGTAGATTGATCATGACCAGCTCCACCGACGACGACAAAGTCCTTAGATCTAATGATGTTTATCATGAGACTAATAATTCTAAGCAGTCGCTTGGCGGTCTTAACTTGTTGTGCAACAACAAAGGTGCTGTTCTTGCCAAGGCAGCCACTGTGGTTAAGATATCAGAGCTTGGAGACCTCTCTCAATATAATGACTTCTTCGGAAACTTGGTTTACAAGCCTCCTCCTCCTGTGCTTGATGATCAACATGAAGATCATTTCTCTCAGATGCCTTTAGTTGCTGCTCAGGTGACCAAGTTTGGATGCGGAGGGTACTCAATTGGCATTGGCATCAGCCACTCGTTGTTTGATGGACCAGCTGCATATGAGTTTATGTGTGCATGGGCTTCTAATTCTGCTATCAAACCAAATAAAAACAAATTAGACGTCATAATGGATGATGAGCTGCAGCCTAAACCAGTAGTACTGGTGCACGATAGAGACGCACTGTTGATGATGGTTGACAATTATTCATGTCATGATGACGATAACGATGACGATGAAGGGGTTAGCTGTAGGGCTGCTGCCATAGATCATTTATATCAACTGATAATGCAATCAGCAGCAGCAGTTGCATCCACTGATCTCCTGATGATCAAGAGTAGCAATGATGATGATAATTATGTTCACAAGACCTTTCATTTGAGCAGTGCATTCCTCAACTGTTTGAAGAAGCAGGTTGTTGGTGACAACTTTTCATGTTCCTCCTTTGAACTTGTAGCAGCTCACCTCTGGAAGGCGAAGACTTTGGCTTTAGGATTGAGCATGGAAACAATGGTGTGCTTGCAATTTGCTGTGGACGCGAGGAACAAGGTGGACCCTCCACTTCCTCAAGGTTTCACCGGCAACGCTTACGTCCTGGCCTCTGTTGCGCTCACTGCCTCACAGGTTGTTGACCAATCCGACCACAAAGCCATTGTTGACAAGATAAGAGAAGCCAAGAAGTCTGTGACAAACAAGTACGTCAAGTCGTACATGGAGGCGGTTGCAGGTGGTGGATCCCAGCAGACTTCTCTACCTCCTCTCAAGGAGCTCACCTTAGTGTCTGATTGGACCAGAATGCCCTTCCACAAGGTTGGTTTCTTACAAGGAGAAGCAGATTATGCTTCTCCGTTGGTCGCTCCAGTGACAGAGGTTATTTATTTCATGCAGAATCCAAAGGATGACAAGGGCATTGACATGAGAATTGGATTGCTTCCGCAATACCACGATGCCTTCACTCGCTATTTCCTCACCAATACCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1308

Protein Analysis

526

Amino Acids

58.0

Weight (kDa)

5.16

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 127 - 511 1.3e-46 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1313
AasI GACNNNNNNGTC 1 cut(s) 332
AatII GACGTC 1 cut(s) 723
Acc36I ACCTGC 2 cut(s) 1000, 1313
Acc65I GGTACC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 282
AccII CGCG 1 cut(s) 1136
AciI CCGC 6 cut(s) 142, 290, 390, 600, 1316, 1532
AclWI GGATC 2 cut(s) 1326, 1339
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 216
AcyI GRCGYC 1 cut(s) 720
AfaI GTAC 8 cut(s) 13, 78, 164, 284, 608, 759, 1295, 1307
AfiI CCNNNNNNNGG 2 cut(s) 293, 1069
AgsI TTSAA 5 cut(s) 63, 242, 250, 1003, 1046
AjnI CCWGG 1 cut(s) 1191
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AleI CACNNNNGTG 1 cut(s) 1373
AluBI AGCT 8 cut(s) 246, 315, 463, 653, 739, 846, 1058, 1366
AluI AGCT 8 cut(s) 246, 315, 463, 653, 739, 846, 1058, 1366
Alw21I GWGCWC 2 cut(s) 768, 1368
Alw26I GTCTC 4 cut(s) 260, 356, 462, 768
Alw44I GTGCAC 1 cut(s) 764
AlwI GGATC 2 cut(s) 1326, 1339
AlwNI CAGNNNCTG 1 cut(s) 443
AoxI GGCC 2 cut(s) 3, 1194
ApaLI GTGCAC 1 cut(s) 764
ApoI RAATTY 1 cut(s) 216
ArsI GACNNNNNNTTYG 4 cut(s) 705, 737, 958, 990
Asp718I GGTACC 1 cut(s) 282
AspLEI GCGC 1 cut(s) 1207
AspS9I GGNCC 4 cut(s) 139, 647, 1150, 1386
AsuHPI GGTGA 8 cut(s) 35, 58, 595, 1031, 1052, 1165, 1360, 1558
AvaII GGWCC 4 cut(s) 139, 647, 1150, 1386
BaeGI GKGCMC 1 cut(s) 768
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 1331
BanI GGYRCC 1 cut(s) 282
BanII GRGCYC 1 cut(s) 1368
BbsI GAAGAC 2 cut(s) 78, 1082
Bbv12I GWGCWC 2 cut(s) 768, 1368
BccI CCATC 2 cut(s) 638, 784
BciT130I CCWGG 1 cut(s) 1193
BclI TGATCA 7 cut(s) 25, 31, 48, 205, 303, 535, 927
BcoDI GTCTC 4 cut(s) 260, 356, 462, 768
BfmI CTRYAG 2 cut(s) 740, 847
BfuAI ACCTGC 2 cut(s) 1000, 1313
BglII AGATCT 1 cut(s) 341
BmcAI AGTACT 2 cut(s) 13, 759
Bme1390I CCNGG 1 cut(s) 1193
Bme18I GGWCC 4 cut(s) 139, 647, 1150, 1386
BmgT120I GGNCC 4 cut(s) 139, 647, 1150, 1386
BmiI GGNNCC 3 cut(s) 284, 1152, 1333
BmrFI CCNGG 1 cut(s) 1193
BmsI GCATC 5 cut(s) 552, 587, 633, 917, 1534
BpiI GAAGAC 2 cut(s) 78, 1082
BpmI CTGGAG 1 cut(s) 1440
Bpu10I CCTNAGC 1 cut(s) 579
BpuEI CTTGAG 2 cut(s) 1149, 1343
BsaBI GATNNNNATC 3 cut(s) 30, 210, 354
BsaHI GRCGYC 1 cut(s) 720
BsaI GGTCTC 1 cut(s) 462
BsaJI CCNNGG 1 cut(s) 432
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 1069
Bse118I RCCGGY 1 cut(s) 1175
Bse1I ACTGG 7 cut(s) 6, 84, 95, 145, 752, 765, 1457
Bse3DI GCAATG 1 cut(s) 946
Bse8I GATNNNNATC 3 cut(s) 30, 210, 354
BseBI CCWGG 1 cut(s) 1193
BseDI CCNNGG 1 cut(s) 432
BseGI GGATG 4 cut(s) 602, 736, 908, 1501
BseJI GATNNNNATC 3 cut(s) 30, 210, 354
BseLI CCNNNNNNNGG 2 cut(s) 293, 1069
BseMI GCAATG 1 cut(s) 946
BseMII CTCAG 2 cut(s) 572, 593
BseNI ACTGG 7 cut(s) 6, 84, 95, 145, 752, 765, 1457
BseRI GAGGAG 4 cut(s) 187, 507, 510, 1344
BseSI GKGCMC 1 cut(s) 768
BseYI CCCAGC 2 cut(s) 286, 1335
BsgI GTGCAG 1 cut(s) 75
Bsh1236I CGCG 1 cut(s) 1136
BshFI GGCC 2 cut(s) 5, 1196
BshNI GGYRCC 1 cut(s) 282
BsiHKAI GWGCWC 2 cut(s) 768, 1368
BsiSI CCGG 1 cut(s) 1176
BslI CCNNNNNNNGG 2 cut(s) 293, 1069
BsmAI GTCTC 4 cut(s) 260, 356, 462, 768
BsmBI CGTCTC 1 cut(s) 768
BsmI GAATGC 2 cut(s) 986, 1398
BsnI GGCC 2 cut(s) 5, 1196
Bso31I GGTCTC 1 cut(s) 462
Bsp1286I GDGCHC 2 cut(s) 768, 1368
BspACI CCGC 6 cut(s) 142, 290, 390, 600, 1316, 1532
BspANI GGCC 2 cut(s) 5, 1196
BspCNI CTCAG 2 cut(s) 571, 592
BspFNI CGCG 1 cut(s) 1136
BspHI TCATGA 5 cut(s) 28, 34, 306, 358, 811
BspLI GGNNCC 3 cut(s) 284, 1152, 1333
BspMAI CTGCAG 1 cut(s) 744
BspMI ACCTGC 2 cut(s) 1000, 1313
BspPI GGATC 2 cut(s) 1326, 1339
BspT107I GGYRCC 1 cut(s) 282
BspTNI GGTCTC 1 cut(s) 462
BsrDI GCAATG 1 cut(s) 946
BsrFI RCCGGY 1 cut(s) 1175
BsrI ACTGG 7 cut(s) 6, 84, 95, 145, 752, 765, 1457
BssAI RCCGGY 1 cut(s) 1175
BssECI CCNNGG 1 cut(s) 432
BssNI GRCGYC 1 cut(s) 720
BssT1I CCWWGG 1 cut(s) 432
Bst2UI CCWGG 1 cut(s) 1193
Bst4CI ACNGT 4 cut(s) 76, 445, 783, 998
Bst6I CTCTTC 1 cut(s) 258
BstACI GRCGYC 1 cut(s) 720
BstAPI GCANNNNNTGC 2 cut(s) 905, 1436
BstC8I GCNNGC 1 cut(s) 1118
BstDEI CTNAG 5 cut(s) 338, 375, 558, 579, 1372
BstEII GGTNACC 1 cut(s) 583
BstENI CCTNNNNNAGG 1 cut(s) 1067
BstF5I GGATG 4 cut(s) 602, 736, 908, 1501
BstFNI CGCG 1 cut(s) 1136
BstHHI GCGC 1 cut(s) 1207
BstMAI GTCTC 4 cut(s) 260, 356, 462, 768
BstMWI GCNNNNNNNGC 7 cut(s) 428, 437, 905, 1133, 1202, 1211, 1436
BstNI CCWGG 1 cut(s) 1193
BstPI GGTNACC 1 cut(s) 583
BstSCI CCNGG 1 cut(s) 1191
BstSFI CTRYAG 2 cut(s) 740, 847
BstSLI GKGCMC 1 cut(s) 768
BstUI CGCG 1 cut(s) 1136
BstV2I GAAGAC 2 cut(s) 78, 1082
BstX2I RGATCY 2 cut(s) 341, 1331
BstXI CCANNNNNNTGG 1 cut(s) 1409
BstYI RGATCY 2 cut(s) 341, 1331
BsuRI GGCC 2 cut(s) 5, 1196
BtsCI GGATG 4 cut(s) 602, 736, 908, 1501
BtsI GCAGTG 2 cut(s) 988, 1209
BtsIMutI CAGTG 7 cut(s) 152, 441, 779, 912, 988, 1209, 1464
BveI ACCTGC 2 cut(s) 1000, 1313
Cac8I GCNNGC 1 cut(s) 1118
CaiI CAGNNNCTG 1 cut(s) 443
CciI TCATGA 5 cut(s) 28, 34, 306, 358, 811
CfoI GCGC 1 cut(s) 1207
Cfr10I RCCGGY 1 cut(s) 1175
Cfr13I GGNCC 4 cut(s) 139, 647, 1150, 1386
CseI GACGC 2 cut(s) 785, 1142
Csp6I GTAC 8 cut(s) 12, 77, 163, 283, 607, 758, 1294, 1306
CviQI GTAC 8 cut(s) 12, 77, 163, 283, 607, 758, 1294, 1306
DdeI CTNAG 5 cut(s) 338, 375, 558, 579, 1372
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Ecl136II GAGCTC 1 cut(s) 1366
Eco130I CCWWGG 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 1368
Eco31I GGTCTC 1 cut(s) 462
Eco32I GATATC 1 cut(s) 456
Eco47I GGWCC 4 cut(s) 139, 647, 1150, 1386
Eco53kI GAGCTC 1 cut(s) 1366
Eco91I GGTNACC 1 cut(s) 583
EcoICRI GAGCTC 1 cut(s) 1366
EcoNI CCTNNNNNAGG 1 cut(s) 1067
EcoO65I GGTNACC 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 1191
EcoRV GATATC 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 1368
ErhI CCWWGG 1 cut(s) 432
Esp3I CGTCTC 1 cut(s) 768
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FauI CCCGC 1 cut(s) 283
FauNDI CATATG 1 cut(s) 658
FbaI TGATCA 7 cut(s) 25, 31, 48, 205, 303, 535, 927
FokI GGATG 4 cut(s) 609, 743, 895, 1508
FriOI GRGCYC 1 cut(s) 1368
GlaI GCGC 1 cut(s) 1206
GsaI CCCAGC 2 cut(s) 290, 1339
GsuI CTGGAG 1 cut(s) 1440
HaeIII GGCC 2 cut(s) 5, 1196
HapII CCGG 1 cut(s) 1176
HgaI GACGC 2 cut(s) 785, 1142
HhaI GCGC 1 cut(s) 1207
Hin1I GRCGYC 1 cut(s) 720
Hin6I GCGC 1 cut(s) 1205
HinP1I GCGC 1 cut(s) 1205
HincII GTYRAC 3 cut(s) 796, 1228, 1255
HindII GTYRAC 3 cut(s) 796, 1228, 1255
HindIII AAGCTT 1 cut(s) 244
HinfI GANTC 2 cut(s) 230, 1486
HpaII CCGG 1 cut(s) 1176
HphI GGTGA 8 cut(s) 35, 58, 595, 1031, 1052, 1165, 1360, 1558
Hpy166II GTNNAC 9 cut(s) 152, 508, 766, 796, 961, 1132, 1150, 1228, 1255
Hpy188I TCNGA 5 cut(s) 460, 497, 561, 1237, 1381
Hpy188III TCNNGA 8 cut(s) 29, 35, 307, 359, 812, 922, 931, 1066
Hpy8I GTNNAC 9 cut(s) 152, 508, 766, 796, 961, 1132, 1150, 1228, 1255
Hpy99I CGWCG 2 cut(s) 326, 329
HpyAV CCTTC 4 cut(s) 830, 1063, 1408, 1558
HpyCH4III ACNGT 4 cut(s) 76, 445, 783, 998
HpyCH4IV ACGT 3 cut(s) 720, 1188, 1296
HpyF10VI GCNNNNNNNGC 7 cut(s) 428, 437, 905, 1133, 1202, 1211, 1436
HpyF3I CTNAG 5 cut(s) 338, 375, 558, 579, 1372
HpySE526I ACGT 3 cut(s) 720, 1188, 1296
Hsp92I GRCGYC 1 cut(s) 720
HspAI GCGC 1 cut(s) 1205
KpnI GGTACC 1 cut(s) 286
Ksp22I TGATCA 7 cut(s) 25, 31, 48, 205, 303, 535, 927
LmnI GCTCC 3 cut(s) 320, 1363, 1459
LweI GCATC 5 cut(s) 552, 587, 633, 917, 1534
MaeII ACGT 3 cut(s) 720, 1188, 1296
MaeIII GTNAC 4 cut(s) 583, 1019, 1282, 1459
MboII GAAGA 6 cut(s) 78, 275, 484, 557, 1015, 1087
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 2 cut(s) 341, 1331
MhlI GDGCHC 2 cut(s) 768, 1368
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 1260
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 396, 450
MslI CAYNNNNRTG 3 cut(s) 33, 1373, 1511
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 290, 653
MspI CCGG 1 cut(s) 1176
MspR9I CCNGG 1 cut(s) 1193
MunI CAATTG 1 cut(s) 612
Mva1269I GAATGC 2 cut(s) 986, 1398
MvaI CCWGG 1 cut(s) 1193
MvnI CGCG 1 cut(s) 1136
MwoI GCNNNNNNNGC 7 cut(s) 428, 437, 905, 1133, 1202, 1211, 1436
NdeI CATATG 1 cut(s) 658
NlaIV GGNNCC 3 cut(s) 284, 1152, 1333
NmuCI GTSAC 4 cut(s) 583, 1019, 1282, 1459
OliI CACNNNNGTG 1 cut(s) 1373
PagI TCATGA 5 cut(s) 28, 34, 306, 358, 811
PaqCI CACCTGC 1 cut(s) 1313
PctI GAATGC 2 cut(s) 986, 1398
PfeI GAWTC 2 cut(s) 230, 1486
PflFI GACNNNGTC 1 cut(s) 332
Psp124BI GAGCTC 1 cut(s) 1368
Psp6I CCWGG 1 cut(s) 1191
PspEI GGTNACC 1 cut(s) 583
PspFI CCCAGC 2 cut(s) 286, 1335
PspGI CCWGG 1 cut(s) 1191
PspN4I GGNNCC 3 cut(s) 284, 1152, 1333
PspPI GGNCC 4 cut(s) 139, 647, 1150, 1386
PstI CTGCAG 1 cut(s) 744
PstNI CAGNNNCTG 1 cut(s) 443
PsuI RGATCY 2 cut(s) 341, 1331
PsyI GACNNNGTC 1 cut(s) 332
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 8 cut(s) 13, 78, 164, 284, 608, 759, 1295, 1307
RsaNI GTAC 8 cut(s) 12, 77, 163, 283, 607, 758, 1294, 1306
RseI CAYNNNNRTG 3 cut(s) 33, 1373, 1511
SacI GAGCTC 1 cut(s) 1368
SaqAI TTAA 2 cut(s) 396, 450
Sau96I GGNCC 4 cut(s) 139, 647, 1150, 1386
ScaI AGTACT 2 cut(s) 13, 759
ScrFI CCNGG 1 cut(s) 1193
SduI GDGCHC 2 cut(s) 768, 1368
SfaNI GCATC 5 cut(s) 552, 587, 633, 917, 1534
SfcI CTRYAG 2 cut(s) 740, 847
SinI GGWCC 4 cut(s) 139, 647, 1150, 1386
SmiMI CAYNNNNRTG 3 cut(s) 33, 1373, 1511
SmlI CTYRAG 2 cut(s) 1164, 1358
SmoI CTYRAG 2 cut(s) 1164, 1358
SsiI CCGC 6 cut(s) 142, 290, 390, 600, 1316, 1532
SstI GAGCTC 1 cut(s) 1368
StyD4I CCNGG 1 cut(s) 1191
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 4 cut(s) 76, 445, 783, 998
TaiI ACGT 3 cut(s) 723, 1191, 1299
TaqI TCGA 1 cut(s) 214
TatI WGTACW 3 cut(s) 11, 162, 757
TfiI GAWTC 2 cut(s) 230, 1486
Tru1I TTAA 2 cut(s) 396, 450
Tru9I TTAA 2 cut(s) 396, 450
TscAI CASTG 7 cut(s) 152, 448, 786, 919, 988, 1216, 1464
TseFI GTSAC 4 cut(s) 583, 1019, 1282, 1459
Tsp45I GTSAC 4 cut(s) 583, 1019, 1282, 1459
TspDTI ATGAA 7 cut(s) 54, 558, 795, 849, 962, 1020, 1468
TspGWI ACGGA 1 cut(s) 1434
TspRI CASTG 7 cut(s) 152, 448, 786, 919, 988, 1216, 1464
Tth111I GACNNNGTC 1 cut(s) 332
VneI GTGCAC 1 cut(s) 764
VpaK11BI GGWCC 4 cut(s) 139, 647, 1150, 1386
XagI CCTNNNNNAGG 1 cut(s) 1067
XapI RAATTY 1 cut(s) 216
ZraI GACGTC 1 cut(s) 721
ZrmI AGTACT 2 cut(s) 13, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.