Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001023

Physical Location & Seq

Genomic Coordinates Reverse (-)

541390 .. 545634

Gene Structure 4245 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1010100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGCACGATTCATTAGATTCTAATTACCGAGACAGTTGTGATGAAGCGCTTTTGAGCTCCACTTGTCCATTCAAAACAGAGATGCCAGAACCAGCTCTTGGATGGCCTCTTCAGAGACTAACTGTTAGTCCTCTGAGGCTGAGCCAGGAAGCTCGCCGAAAATCGGATAGCTTCGACATGTTTTCCGAGAGAGAGGATGATGAATCACCGTTGTCGAGGCCGGGTTGGCCTCTCTTGCGGATAGCAGCTGCCTCAGCGGAAACTATGAGTACCACTCCTATGAGAGAATTCAAATCCAGAGAAAACATGTCCGTGGTACAGTGGGTGATGATGGACCTGCCTAGGCGTCGATTTCTTTCGGTAAATTCACAGCCGCAGGAAGGTGATGAAATTTGCTCGACAGTAGCACCAACACAGCTTTCTGATGAATTGGAGTTTCTCACCGCAGAAAACTCAGAAGGTTGCAAGGTTTTCAGCTACGCGGAACTTAGTAGCGCAACTTGTCACTTCTCGACAGAAAATCTTATCGGCGAGGGAGGGTGCAGCAGCGTGTACAAAGGGTGCCTTGGAGGCGGAGAATCAGTGGCGGTCAAGGTTCTAAAATCGTACAAGGAAGCTTGGAATGACTTTTTCTTGGAAGCCAAGTTTGTTTCTTCAATTCAGCACAAACACATCACTTGTCCAATTGGTGTATGCATGGAAGATGGCAACCTTATTTTGGTCTATGACCTCTTTCCCAGAGGGAGCTTGGAAGGAAACTTGCACGGTTGCAGTGATGGACTTATACTTCCATGGAGCGTGAGATTCAATGTGGCGGTTGCAGTTGCAGAGGCTCTAACTTACCTGCACAATGAGTGCCCTCAGCCGATCATTCACCGAGACGTTAAATCCTCAAACATTCTTCTCTCCGACGACCTTCAGCCGCAGTTATCTGATTTCGGGCTTGCTACATGGGGACCGATGGATTCTGGTTGCGTGATAAGTAGTGATGTGGTGGGAACTTTTGGATACATTGCTCCGGAATATTTCATGCATGGGGTGGTGAGTGACAAGATTGATGTTTATGCCTTCGGCGTGGTTCTCCTCGAACTATTATCTGGTCGAAAGCCGGTCGATGCCGAGGCTCTGAAAGGACAAGAGAGTTTGGTCAAGTGGGCAAGACATTCGTTAGATATTATGGATCTTAAGGCATTGCTGGATCCAAAGTTAAACGGAGACTACGATGCGGCCCAAATGCGTAGAATGGTAATGGCAGCAGGGCTTTGCATAAACCAGTCGCCTCGTCGTCGACCGGAAGCGAGACAAGTACTGAAGCTACTAACGGGAGAGACGGACGCAAAAGAGTGCGTCGATCTTCATGCGGTGAAATCGAGCGACGCGTGCTATCAAGATGAAGATGACAACCAATTCCTGGAAATCGGGCATCTTTCGGTTTCCGCTATGTCTGATACAAATGATGGGAGCATCAGCAGATCAAGTTCAACTGATACTGCATCTAGTGCAGAGAAACCACACCGGTACAAGTTGAAGGACTACTTGCTTATCCCTGACCGGTATTCTTTGCGTGAAATTTCTCCGATAAACATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009623 GO:0009719 GO:0009723 GO:0009725 GO:0009814 GO:0009987 GO:0010033 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0023052 GO:0032870 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

530

Amino Acids

58.65

Weight (kDa)

4.89

Isoelectric Point (pI)

52.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	171 - 438	5.5e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	172 - 438	1.2e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16750 AT2G16750 AT4G35030 AT4G35030 AT4G35030 AT4G35030 AT4G35030 AT4G35030 AT4G35030 AT4G35030 AT4G35030
fragaria_vesca	FvH4_2g01230 FvH4_2g01230 FvH4_2g01230 FvH4_2g01230 FvH4_2g01230 FvH4_2g01230 FvH4_2g01230 FvH4_2g01230 FvH4_2g01230 FvH4_2g01230 FvH4_2g01230 FvH4_2g01230 FvH4_2g01230 FvH4_2g38170 FvH4_2g38170
malus_domestica	MD05G1121300.v1.1 MD08G1010200.v1.1 MD15G1010100.v1.1
prunus_persica	Prupe.1G362600_v2.0.a1 Prupe.8G165200_v2.0.a1 Prupe.8G165200_v2.0.a1
pyrus_communis	pycom05g11550 pycom10g10680 pycom15g00830
rosa_chinensis	RchiOBHm_Chr6g0251891 RchiOBHm_Chr6g0303311
rosa_laevigata	RLG00000011093 RLG00000015422
rosa_multiflora	Rmu_sc0007252.1_g000005 Rmu_sc0027227.1_g000001 Rmu_ssc0000303.1_g000011
rosa_roxburghii	Rroxscaffold_7G00164880 Rroxscaffold_7G00216330
rosa_rugosa	Rorug05G0504900 Rorug05G0504900 Rorug06G0320200 Rorug06G0320300 Rorug06G0320300 Rorug06G0320300 Rorug06G0320300
rosa_samantha	Rh6AG016000 Rh6AG434700 Rh6BG014000 Rh6BG468400 Rh6CG010900 Rh6CG446700 Rh6DG012100 Rh6DG433500
rosa_wichuraiana	Rw6G001530 Rw6G037640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	345, 852
AccB1I	GGYRCC	1 cut(s)	561
AccB7I	CCANNNNNTGG	2 cut(s)	98, 1411
AccI	GTMKAC	1 cut(s)	1288
AccII	CGCG	2 cut(s)	482, 1379
AccIII	TCCGGA	1 cut(s)	1018
AclWI	GGATC	3 cut(s)	1188, 1193, 1206
AcsI	RAATTY	4 cut(s)	287, 364, 390, 1569
AcuI	CTGAAG	3 cut(s)	95, 902, 1331
AcyI	GRCGYC	1 cut(s)	346
AfaI	GTAC	6 cut(s)	271, 318, 554, 608, 1308, 1520
AfeI	AGCGCT	1 cut(s)	48
AfiI	CCNNNNNNNGG	5 cut(s)	98, 163, 380, 718, 1411
AflII	CTTAAG	1 cut(s)	1184
AflIII	ACRYGT	4 cut(s)	177, 306, 1377, 1584
AgeI	ACCGGT	2 cut(s)	1515, 1551
AgsI	TTSAA	6 cut(s)	73, 292, 657, 808, 1482, 1528
AjnI	CCWGG	2 cut(s)	144, 1410
AjuI	GAANNNNNNNTTGG	4 cut(s)	81, 113, 403, 435
Alw21I	GWGCWC	1 cut(s)	59
Alw26I	GTCTC	6 cut(s)	24, 109, 873, 1209, 1294, 1322
AlwI	GGATC	3 cut(s)	1188, 1193, 1206
Aor13HI	TCCGGA	1 cut(s)	1018
Aor51HI	AGCGCT	1 cut(s)	48
AoxI	GGCC	4 cut(s)	104, 218, 227, 1227
ApeKI	GCWGC	5 cut(s)	245, 248, 543, 546, 1253
ApoI	RAATTY	4 cut(s)	287, 364, 390, 1569
AsiGI	ACCGGT	2 cut(s)	1515, 1551
AspA2I	CCTAGG	1 cut(s)	341
AspLEI	GCGC	2 cut(s)	49, 497
AspS9I	GGNCC	3 cut(s)	334, 956, 1228
AsuC2I	CCSGG	1 cut(s)	222
AsuHPI	GGTGA	7 cut(s)	198, 337, 395, 433, 866, 1054, 1375
AvaII	GGWCC	2 cut(s)	334, 956
AvrII	CCTAGG	1 cut(s)	341
BaeGI	GKGCMC	1 cut(s)	860
BamHI	GGATCC	1 cut(s)	1198
BanI	GGYRCC	1 cut(s)	561
BanII	GRGCYC	1 cut(s)	59
Bbv12I	GWGCWC	1 cut(s)	59
BbvCI	CCTCAGC	2 cut(s)	253, 861
BbvI	GCAGC	5 cut(s)	235, 257, 555, 558, 1265
BccI	CCATC	6 cut(s)	96, 325, 698, 770, 955, 1451
BcgI	CGANNNNNNTGC	4 cut(s)	1146, 1180, 1340, 1374
BciT130I	CCWGG	2 cut(s)	146, 1412
BciVI	GTATCC	1 cut(s)	1001
BcnI	CCSGG	1 cut(s)	222
BcoDI	GTCTC	6 cut(s)	24, 109, 873, 1209, 1294, 1322
BfaI	CTAG	2 cut(s)	342, 1497
BfoI	RGCGCY	1 cut(s)	50
BfrI	CTTAAG	1 cut(s)	1184
BfuAI	ACCTGC	2 cut(s)	345, 852
BfuI	GTATCC	1 cut(s)	1001
BglI	GCCNNNNNGGC	2 cut(s)	226, 570
BisI	GCNGC	8 cut(s)	246, 249, 374, 544, 547, 923, 1227, 1254
BlnI	CCTAGG	1 cut(s)	341
BlpI	GCTNAGC	1 cut(s)	140
BlsI	GCNGC	8 cut(s)	247, 250, 375, 545, 548, 924, 1228, 1255
BmcAI	AGTACT	1 cut(s)	1308
Bme1390I	CCNGG	3 cut(s)	146, 222, 1412
Bme18I	GGWCC	2 cut(s)	334, 956
BmgT120I	GGNCC	3 cut(s)	334, 956, 1228
BmiI	GGNNCC	3 cut(s)	563, 957, 1200
BmrFI	CCNGG	3 cut(s)	146, 222, 1412
BmsI	GCATC	6 cut(s)	72, 1105, 1213, 1432, 1473, 1502
BplI	GAGNNNNNCTC	2 cut(s)	259, 291
Bpu10I	CCTNAGC	2 cut(s)	253, 861
Bpu1102I	GCTNAGC	1 cut(s)	140
BpuMI	CCSGG	1 cut(s)	222
BsaHI	GRCGYC	1 cut(s)	346
BsaJI	CCNNGG	5 cut(s)	312, 341, 565, 791, 1119
BsaWI	WCCGGW	4 cut(s)	1018, 1291, 1515, 1551
BsaXI	ACNNNNNCTCC	2 cut(s)	567, 597
Bsc4I	CCNNNNNNNGG	5 cut(s)	98, 163, 380, 718, 1411
Bse118I	RCCGGY	3 cut(s)	1108, 1515, 1551
Bse1I	ACTGG	1 cut(s)	1273
Bse3DI	GCAATG	2 cut(s)	1011, 1190
BseAI	TCCGGA	1 cut(s)	1018
BseBI	CCWGG	2 cut(s)	146, 1412
BseDI	CCNNGG	5 cut(s)	312, 341, 565, 791, 1119
BseGI	GGATG	2 cut(s)	107, 202
BseLI	CCNNNNNNNGG	5 cut(s)	98, 163, 380, 718, 1411
BseMI	GCAATG	2 cut(s)	1011, 1190
BseMII	CTCAG	5 cut(s)	125, 131, 267, 468, 875
BseNI	ACTGG	1 cut(s)	1273
BseRI	GAGGAG	1 cut(s)	1073
BseSI	GKGCMC	1 cut(s)	860
BseXI	GCAGC	5 cut(s)	235, 257, 555, 558, 1265
BsgI	GTGCAG	3 cut(s)	562, 830, 1521
Bsh1236I	CGCG	2 cut(s)	482, 1379
Bsh1285I	CGRYCG	2 cut(s)	1113, 1292
BshFI	GGCC	4 cut(s)	106, 220, 229, 1229
BshNI	GGYRCC	1 cut(s)	561
BshTI	ACCGGT	2 cut(s)	1515, 1551
BsiEI	CGRYCG	2 cut(s)	1113, 1292
BsiHKAI	GWGCWC	1 cut(s)	59
BsiSI	CCGG	6 cut(s)	221, 1019, 1109, 1292, 1516, 1552
BslFI	GGGAC	1 cut(s)	969
BslI	CCNNNNNNNGG	5 cut(s)	98, 163, 380, 718, 1411
BsmAI	GTCTC	6 cut(s)	24, 109, 873, 1209, 1294, 1322
BsmBI	CGTCTC	2 cut(s)	873, 1322
BsmFI	GGGAC	1 cut(s)	969
BsnI	GGCC	4 cut(s)	106, 220, 229, 1229
Bsp1286I	GDGCHC	2 cut(s)	59, 860
Bsp13I	TCCGGA	1 cut(s)	1018
Bsp1407I	TGTACA	1 cut(s)	552
Bsp143I	GATC	5 cut(s)	867, 1180, 1198, 1351, 1472
Bsp1720I	GCTNAGC	1 cut(s)	140
Bsp19I	CCATGG	1 cut(s)	791
BspANI	GGCC	4 cut(s)	106, 220, 229, 1229
BspCNI	CTCAG	5 cut(s)	126, 132, 266, 467, 874
BspEI	TCCGGA	1 cut(s)	1018
BspFNI	CGCG	2 cut(s)	482, 1379
BspLI	GGNNCC	3 cut(s)	563, 957, 1200
BspMI	ACCTGC	2 cut(s)	345, 852
BspPI	GGATC	3 cut(s)	1188, 1193, 1206
BspT107I	GGYRCC	1 cut(s)	561
BspTI	CTTAAG	1 cut(s)	1184
BsrDI	GCAATG	2 cut(s)	1011, 1190
BsrFI	RCCGGY	3 cut(s)	1108, 1515, 1551
BsrGI	TGTACA	1 cut(s)	552
BsrI	ACTGG	1 cut(s)	1273
BssAI	RCCGGY	3 cut(s)	1108, 1515, 1551
BssECI	CCNNGG	5 cut(s)	312, 341, 565, 791, 1119
BssMI	GATC	5 cut(s)	867, 1180, 1198, 1351, 1472
BssNI	GRCGYC	1 cut(s)	346
BssT1I	CCWWGG	3 cut(s)	341, 565, 791
Bst2UI	CCWGG	2 cut(s)	146, 1412
Bst4CI	ACNGT	6 cut(s)	35, 124, 210, 321, 403, 767
Bst6I	CTCTTC	1 cut(s)	114
BstACI	GRCGYC	1 cut(s)	346
BstAFI	CTTAAG	1 cut(s)	1184
BstAPI	GCANNNNNTGC	1 cut(s)	1499
BstAUI	TGTACA	1 cut(s)	552
BstC8I	GCNNGC	3 cut(s)	154, 945, 1381
BstDEI	CTNAG	6 cut(s)	134, 140, 253, 454, 488, 861
BstDSI	CCRYGG	2 cut(s)	312, 791
BstF5I	GGATG	2 cut(s)	107, 202
BstFNI	CGCG	2 cut(s)	482, 1379
BstH2I	RGCGCY	1 cut(s)	50
BstHHI	GCGC	2 cut(s)	49, 497
BstKTI	GATC	5 cut(s)	870, 1183, 1201, 1354, 1475
BstMAI	GTCTC	6 cut(s)	24, 109, 873, 1209, 1294, 1322
BstMBI	GATC	5 cut(s)	867, 1180, 1198, 1351, 1472
BstMCI	CGRYCG	2 cut(s)	1113, 1292
BstMWI	GCNNNNNNNGC	6 cut(s)	226, 235, 254, 570, 1380, 1499
BstNI	CCWGG	2 cut(s)	146, 1412
BstNSI	RCATGY	3 cut(s)	181, 310, 1588
BstSCI	CCNGG	3 cut(s)	144, 220, 1410
BstSLI	GKGCMC	1 cut(s)	860
BstUI	CGCG	2 cut(s)	482, 1379
BstV1I	GCAGC	5 cut(s)	235, 257, 555, 558, 1265
BstX2I	RGATCY	2 cut(s)	1180, 1198
BstYI	RGATCY	2 cut(s)	1180, 1198
BsuI	GTATCC	1 cut(s)	1001
BsuRI	GGCC	4 cut(s)	106, 220, 229, 1229
BtgI	CCRYGG	2 cut(s)	312, 791
BtsCI	GGATG	2 cut(s)	107, 202
BtsI	GCAGTG	1 cut(s)	778
BtsIMutI	CAGTG	3 cut(s)	326, 588, 778
BveI	ACCTGC	2 cut(s)	345, 852
Cac8I	GCNNGC	3 cut(s)	154, 945, 1381
CfoI	GCGC	2 cut(s)	49, 497
Cfr10I	RCCGGY	3 cut(s)	1108, 1515, 1551
Cfr13I	GGNCC	3 cut(s)	334, 956, 1228
CseI	GACGC	4 cut(s)	335, 1336, 1343, 1385
Csp6I	GTAC	6 cut(s)	270, 317, 553, 607, 1307, 1519
CspAI	ACCGGT	2 cut(s)	1515, 1551
CviAII	CATG	9 cut(s)	178, 307, 697, 792, 951, 1030, 1034, 1358, 1585
CviQI	GTAC	6 cut(s)	270, 317, 553, 607, 1307, 1519
DdeI	CTNAG	6 cut(s)	134, 140, 253, 454, 488, 861
DpnI	GATC	5 cut(s)	869, 1182, 1200, 1353, 1474
DpnII	GATC	5 cut(s)	867, 1180, 1198, 1351, 1472
Eam1104I	CTCTTC	1 cut(s)	114
EarI	CTCTTC	1 cut(s)	114
EciI	GGCGGA	1 cut(s)	588
Ecl136II	GAGCTC	1 cut(s)	57
Eco130I	CCWWGG	3 cut(s)	341, 565, 791
Eco24I	GRGCYC	1 cut(s)	59
Eco47I	GGWCC	2 cut(s)	334, 956
Eco47III	AGCGCT	1 cut(s)	48
Eco53kI	GAGCTC	1 cut(s)	57
Eco57I	CTGAAG	3 cut(s)	95, 902, 1331
EcoICRI	GAGCTC	1 cut(s)	57
EcoRI	GAATTC	1 cut(s)	287
EcoRII	CCWGG	2 cut(s)	144, 1410
EcoT14I	CCWWGG	3 cut(s)	341, 565, 791
EcoT22I	ATGCAT	2 cut(s)	698, 1035
EcoT38I	GRGCYC	1 cut(s)	59
ErhI	CCWWGG	3 cut(s)	341, 565, 791
Esp3I	CGTCTC	2 cut(s)	873, 1322
FaeI	CATG	9 cut(s)	181, 310, 700, 795, 954, 1033, 1037, 1361, 1588
FalI	AAGNNNNNCTT	4 cut(s)	549, 581, 1520, 1552
FaqI	GGGAC	1 cut(s)	969
FatI	CATG	9 cut(s)	177, 306, 696, 791, 950, 1029, 1033, 1357, 1584
FblI	GTMKAC	1 cut(s)	1288
Fnu4HI	GCNGC	8 cut(s)	246, 249, 374, 544, 547, 923, 1227, 1254
FokI	GGATG	2 cut(s)	114, 209
FriOI	GRGCYC	1 cut(s)	59
Fsp4HI	GCNGC	8 cut(s)	246, 249, 374, 544, 547, 923, 1227, 1254
FspBI	CTAG	2 cut(s)	342, 1497
GlaI	GCGC	2 cut(s)	48, 496
GluI	GCNGC	8 cut(s)	246, 249, 374, 544, 547, 923, 1227, 1254
HaeII	RGCGCY	1 cut(s)	50
HaeIII	GGCC	4 cut(s)	106, 220, 229, 1229
HapII	CCGG	6 cut(s)	221, 1019, 1109, 1292, 1516, 1552
HgaI	GACGC	4 cut(s)	335, 1336, 1343, 1385
HhaI	GCGC	2 cut(s)	49, 497
Hin1I	GRCGYC	1 cut(s)	346
Hin1II	CATG	9 cut(s)	181, 310, 700, 795, 954, 1033, 1037, 1361, 1588
Hin6I	GCGC	2 cut(s)	47, 495
HinP1I	GCGC	2 cut(s)	47, 495
HincII	GTYRAC	1 cut(s)	1289
HindII	GTYRAC	1 cut(s)	1289
HindIII	AAGCTT	1 cut(s)	615
HinfI	GANTC	6 cut(s)	8, 17, 203, 578, 804, 965
HpaII	CCGG	6 cut(s)	221, 1019, 1109, 1292, 1516, 1552
HphI	GGTGA	7 cut(s)	198, 337, 395, 433, 866, 1054, 1375
Hpy166II	GTNNAC	2 cut(s)	553, 1289
Hpy188III	TCNNGA	4 cut(s)	297, 511, 1019, 1388
Hpy8I	GTNNAC	2 cut(s)	553, 1289
Hpy99I	CGWCG	6 cut(s)	351, 914, 1287, 1290, 1352, 1379
HpyAV	CCTTC	6 cut(s)	374, 452, 746, 926, 1078, 1522
HpyCH4III	ACNGT	6 cut(s)	35, 124, 210, 321, 403, 767
HpyCH4IV	ACGT	1 cut(s)	882
HpyF10VI	GCNNNNNNNGC	6 cut(s)	226, 235, 254, 570, 1380, 1499
HpyF3I	CTNAG	6 cut(s)	134, 140, 253, 454, 488, 861
HpySE526I	ACGT	1 cut(s)	882
Hsp92I	GRCGYC	1 cut(s)	346
Hsp92II	CATG	9 cut(s)	181, 310, 700, 795, 954, 1033, 1037, 1361, 1588
HspAI	GCGC	2 cut(s)	47, 495
Kpn2I	TCCGGA	1 cut(s)	1018
Kzo9I	GATC	5 cut(s)	867, 1180, 1198, 1351, 1472
LmnI	GCTCC	5 cut(s)	62, 744, 795, 1021, 1461
Lsp1109I	GCAGC	5 cut(s)	235, 257, 555, 558, 1265
LweI	GCATC	6 cut(s)	72, 1105, 1213, 1432, 1473, 1502
MaeI	CTAG	2 cut(s)	342, 1497
MaeII	ACGT	1 cut(s)	882
MaeIII	GTNAC	2 cut(s)	503, 1046
MalI	GATC	5 cut(s)	869, 1182, 1200, 1353, 1474
MboI	GATC	5 cut(s)	867, 1180, 1198, 1351, 1472
MboII	GAAGA	6 cut(s)	101, 645, 713, 893, 1346, 1406
MfeI	CAATTG	1 cut(s)	684
MflI	RGATCY	2 cut(s)	1180, 1198
MhlI	GDGCHC	2 cut(s)	59, 860
MluCI	AATT	9 cut(s)	22, 287, 364, 390, 428, 657, 684, 1406, 1569
MluI	ACGCGT	1 cut(s)	1377
MmeI	TCCRAC	1 cut(s)	933
Mph1103I	ATGCAT	2 cut(s)	698, 1035
MroI	TCCGGA	1 cut(s)	1018
MseI	TTAA	3 cut(s)	885, 1185, 1208
MslI	CAYNNNNRTG	2 cut(s)	278, 311
MspA1I	CMGCKG	2 cut(s)	248, 257
MspCI	CTTAAG	1 cut(s)	1184
MspI	CCGG	6 cut(s)	221, 1019, 1109, 1292, 1516, 1552
MspR9I	CCNGG	3 cut(s)	146, 222, 1412
MunI	CAATTG	1 cut(s)	684
MvaI	CCWGG	2 cut(s)	146, 1412
MvnI	CGCG	2 cut(s)	482, 1379
MwoI	GCNNNNNNNGC	6 cut(s)	226, 235, 254, 570, 1380, 1499
NciI	CCSGG	1 cut(s)	222
NcoI	CCATGG	1 cut(s)	791
NdeII	GATC	5 cut(s)	867, 1180, 1198, 1351, 1472
NlaIII	CATG	9 cut(s)	181, 310, 700, 795, 954, 1033, 1037, 1361, 1588
NlaIV	GGNNCC	3 cut(s)	563, 957, 1200
NmeAIII	GCCGAG	1 cut(s)	1144
NmuCI	GTSAC	2 cut(s)	503, 1046
NsiI	ATGCAT	2 cut(s)	698, 1035
NspI	RCATGY	3 cut(s)	181, 310, 1588
PciI	ACATGT	3 cut(s)	177, 306, 1584
PcsI	WCGNNNNNNNCGW	2 cut(s)	1218, 1376
PfeI	GAWTC	6 cut(s)	8, 17, 203, 578, 804, 965
PflMI	CCANNNNNTGG	2 cut(s)	98, 1411
PfoI	TCCNGGA	1 cut(s)	1410
PinAI	ACCGGT	2 cut(s)	1515, 1551
PkrI	GCNGC	8 cut(s)	247, 250, 375, 545, 548, 924, 1228, 1255
PscI	ACATGT	3 cut(s)	177, 306, 1584
Psp124BI	GAGCTC	1 cut(s)	59
Psp6I	CCWGG	2 cut(s)	144, 1410
PspGI	CCWGG	2 cut(s)	144, 1410
PspN4I	GGNNCC	3 cut(s)	563, 957, 1200
PspPI	GGNCC	3 cut(s)	334, 956, 1228
PsuI	RGATCY	2 cut(s)	1180, 1198
PvuII	CAGCTG	1 cut(s)	248
RsaI	GTAC	6 cut(s)	271, 318, 554, 608, 1308, 1520
RsaNI	GTAC	6 cut(s)	270, 317, 553, 607, 1307, 1519
RseI	CAYNNNNRTG	2 cut(s)	278, 311
SacI	GAGCTC	1 cut(s)	59
SalI	GTCGAC	1 cut(s)	1287
SaqAI	TTAA	3 cut(s)	885, 1185, 1208
SatI	GCNGC	8 cut(s)	246, 249, 374, 544, 547, 923, 1227, 1254
Sau3AI	GATC	5 cut(s)	867, 1180, 1198, 1351, 1472
Sau96I	GGNCC	3 cut(s)	334, 956, 1228
ScaI	AGTACT	1 cut(s)	1308
ScrFI	CCNGG	3 cut(s)	146, 222, 1412
SduI	GDGCHC	2 cut(s)	59, 860
SfaNI	GCATC	6 cut(s)	72, 1105, 1213, 1432, 1473, 1502
SfiI	GGCCNNNNNGGCC	1 cut(s)	226
SinI	GGWCC	2 cut(s)	334, 956
SmiMI	CAYNNNNRTG	2 cut(s)	278, 311
SmlI	CTYRAG	1 cut(s)	1184
SmoI	CTYRAG	1 cut(s)	1184
Sse9I	AATT	9 cut(s)	22, 287, 364, 390, 428, 657, 684, 1406, 1569
SspI	AATATT	1 cut(s)	1025
SspMI	CTAG	2 cut(s)	342, 1497
SstI	GAGCTC	1 cut(s)	59
StyD4I	CCNGG	3 cut(s)	144, 220, 1410
StyI	CCWWGG	3 cut(s)	341, 565, 791
TaaI	ACNGT	6 cut(s)	35, 124, 210, 321, 403, 767
TaiI	ACGT	1 cut(s)	885
TaqII	GACCGA	1 cut(s)	973
TasI	AATT	9 cut(s)	22, 287, 364, 390, 428, 657, 684, 1406, 1569
TatI	WGTACW	2 cut(s)	552, 1306
TauI	GCSGC	3 cut(s)	376, 925, 1229
TfiI	GAWTC	6 cut(s)	8, 17, 203, 578, 804, 965
Tru1I	TTAA	3 cut(s)	885, 1185, 1208
Tru9I	TTAA	3 cut(s)	885, 1185, 1208
TscAI	CASTG	3 cut(s)	326, 588, 778
TseFI	GTSAC	2 cut(s)	503, 1046
TseI	GCWGC	5 cut(s)	245, 248, 543, 546, 1253
Tsp45I	GTSAC	2 cut(s)	503, 1046
TspDTI	ATGAA	7 cut(s)	57, 216, 402, 441, 1018, 1346, 1407
TspGWI	ACGGA	3 cut(s)	301, 1227, 1346
TspRI	CASTG	3 cut(s)	326, 588, 778
Van91I	CCANNNNNTGG	2 cut(s)	98, 1411
Vha464I	CTTAAG	1 cut(s)	1184
VpaK11BI	GGWCC	2 cut(s)	334, 956
XapI	RAATTY	4 cut(s)	287, 364, 390, 1569
XceI	RCATGY	3 cut(s)	181, 310, 1588
XcmI	CCANNNNNNNNNTGG	2 cut(s)	99, 745
XmaJI	CCTAGG	1 cut(s)	341
XmiI	GTMKAC	1 cut(s)	1288
XspI	CTAG	2 cut(s)	342, 1497
ZrmI	AGTACT	1 cut(s)	1308
Zsp2I	ATGCAT	2 cut(s)	698, 1035

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.