Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 531 |
| AarI | CACCTGC | 1 cut(s) | 485 |
| Acc36I | ACCTGC | 2 cut(s) | 485, 1359 |
| AccI | GTMKAC | 2 cut(s) | 258, 1137 |
| AccII | CGCG | 1 cut(s) | 125 |
| AciI | CCGC | 2 cut(s) | 158, 581 |
| AclWI | GGATC | 2 cut(s) | 1829, 1842 |
| AcsI | RAATTY | 5 cut(s) | 563, 1000, 1056, 1252, 1711 |
| AcuI | CTGAAG | 4 cut(s) | 156, 680, 993, 1356 |
| AfaI | GTAC | 4 cut(s) | 469, 898, 1692, 1743 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 368, 1155, 1181, 1831, 2020 |
| AflIII | ACRYGT | 2 cut(s) | 792, 1258 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 53, 85, 1005, 1037 |
| AleI | CACNNNNGTG | 2 cut(s) | 425, 1499 |
| Alw21I | GWGCWC | 3 cut(s) | 626, 682, 1167 |
| Alw26I | GTCTC | 3 cut(s) | 138, 849, 944 |
| AlwI | GGATC | 2 cut(s) | 1829, 1842 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 593, 1744 |
| Ama87I | CYCGRG | 1 cut(s) | 778 |
| AoxI | GGCC | 3 cut(s) | 509, 740, 1447 |
| ApeKI | GCWGC | 3 cut(s) | 131, 1319, 1760 |
| ApoI | RAATTY | 5 cut(s) | 563, 1000, 1056, 1252, 1711 |
| AspLEI | GCGC | 2 cut(s) | 304, 919 |
| AspS9I | GGNCC | 1 cut(s) | 1463 |
| AsuC2I | CCSGG | 2 cut(s) | 779, 780 |
| AsuHPI | GGTGA | 3 cut(s) | 509, 581, 823 |
| AvaI | CYCGRG | 1 cut(s) | 778 |
| AvaII | GGWCC | 1 cut(s) | 1463 |
| BaeGI | GKGCMC | 1 cut(s) | 785 |
| BamHI | GGATCC | 1 cut(s) | 1834 |
| BanII | GRGCYC | 3 cut(s) | 121, 626, 682 |
| BauI | CACGAG | 2 cut(s) | 974, 1160 |
| BbsI | GAAGAC | 4 cut(s) | 660, 1092, 1389, 1470 |
| Bbv12I | GWGCWC | 3 cut(s) | 626, 682, 1167 |
| BbvI | GCAGC | 3 cut(s) | 118, 1306, 1772 |
| BccI | CCATC | 6 cut(s) | 108, 732, 957, 1061, 1712, 2037 |
| BciVI | GTATCC | 1 cut(s) | 577 |
| BclI | TGATCA | 1 cut(s) | 181 |
| BcnI | CCSGG | 2 cut(s) | 779, 780 |
| BcoDI | GTCTC | 3 cut(s) | 138, 849, 944 |
| BfaI | CTAG | 7 cut(s) | 305, 491, 543, 633, 1424, 1676, 1788 |
| BfmI | CTRYAG | 3 cut(s) | 339, 384, 1497 |
| BfoI | RGCGCY | 1 cut(s) | 305 |
| BfuAI | ACCTGC | 2 cut(s) | 485, 1359 |
| BfuI | GTATCC | 1 cut(s) | 577 |
| BisI | GCNGC | 3 cut(s) | 132, 1320, 1761 |
| BlpI | GCTNAGC | 1 cut(s) | 1783 |
| BlsI | GCNGC | 3 cut(s) | 133, 1321, 1762 |
| BmcAI | AGTACT | 1 cut(s) | 898 |
| Bme1390I | CCNGG | 2 cut(s) | 779, 780 |
| Bme18I | GGWCC | 1 cut(s) | 1463 |
| BmeT110I | CYCGRG | 1 cut(s) | 778 |
| BmgT120I | GGNCC | 1 cut(s) | 1463 |
| BmiI | GGNNCC | 2 cut(s) | 1464, 1836 |
| BmrFI | CCNGG | 2 cut(s) | 779, 780 |
| BmsI | GCATC | 4 cut(s) | 904, 1025, 1255, 1782 |
| BpiI | GAAGAC | 4 cut(s) | 660, 1092, 1389, 1470 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1783 |
| BpuEI | CTTGAG | 1 cut(s) | 29 |
| BpuMI | CCSGG | 2 cut(s) | 779, 780 |
| BsaJI | CCNNGG | 5 cut(s) | 367, 506, 733, 778, 1430 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 135, 165, 1130, 1160 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 368, 1155, 1181, 1831, 2020 |
| Bse1I | ACTGG | 1 cut(s) | 1373 |
| Bse3DI | GCAATG | 1 cut(s) | 812 |
| BseDI | CCNNGG | 5 cut(s) | 367, 506, 733, 778, 1430 |
| BseGI | GGATG | 4 cut(s) | 119, 743, 919, 1040 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 368, 1155, 1181, 1831, 2020 |
| BseMI | GCAATG | 1 cut(s) | 812 |
| BseMII | CTCAG | 5 cut(s) | 567, 609, 1185, 1736, 1797 |
| BseNI | ACTGG | 1 cut(s) | 1373 |
| BseRI | GAGGAG | 4 cut(s) | 26, 837, 1211, 1262 |
| BseSI | GKGCMC | 1 cut(s) | 785 |
| BseXI | GCAGC | 3 cut(s) | 118, 1306, 1772 |
| BsgI | GTGCAG | 2 cut(s) | 1337, 1953 |
| Bsh1236I | CGCG | 1 cut(s) | 125 |
| BshFI | GGCC | 3 cut(s) | 511, 742, 1449 |
| BsiHKAI | GWGCWC | 3 cut(s) | 626, 682, 1167 |
| BsiHKCI | CYCGRG | 1 cut(s) | 778 |
| BsiSI | CCGG | 2 cut(s) | 141, 779 |
| BslFI | GGGAC | 1 cut(s) | 1476 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 368, 1155, 1181, 1831, 2020 |
| BsmAI | GTCTC | 3 cut(s) | 138, 849, 944 |
| BsmFI | GGGAC | 1 cut(s) | 1476 |
| BsmI | GAATGC | 1 cut(s) | 1496 |
| BsnI | GGCC | 3 cut(s) | 511, 742, 1449 |
| BsoBI | CYCGRG | 1 cut(s) | 778 |
| Bsp1286I | GDGCHC | 5 cut(s) | 121, 626, 682, 785, 1167 |
| Bsp1407I | TGTACA | 2 cut(s) | 467, 1690 |
| Bsp143I | GATC | 5 cut(s) | 181, 689, 826, 1834, 2051 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1783 |
| Bsp68I | TCGCGA | 1 cut(s) | 125 |
| BspACI | CCGC | 2 cut(s) | 158, 581 |
| BspANI | GGCC | 3 cut(s) | 511, 742, 1449 |
| BspCNI | CTCAG | 5 cut(s) | 568, 610, 1186, 1737, 1796 |
| BspFNI | CGCG | 1 cut(s) | 125 |
| BspHI | TCATGA | 1 cut(s) | 1129 |
| BspLI | GGNNCC | 2 cut(s) | 1464, 1836 |
| BspMI | ACCTGC | 2 cut(s) | 485, 1359 |
| BspPI | GGATC | 2 cut(s) | 1829, 1842 |
| BsrDI | GCAATG | 1 cut(s) | 812 |
| BsrGI | TGTACA | 2 cut(s) | 467, 1690 |
| BsrI | ACTGG | 1 cut(s) | 1373 |
| BssECI | CCNNGG | 5 cut(s) | 367, 506, 733, 778, 1430 |
| BssMI | GATC | 5 cut(s) | 181, 689, 826, 1834, 2051 |
| BssSI | CACGAG | 2 cut(s) | 974, 1160 |
| BssT1I | CCWWGG | 2 cut(s) | 367, 506 |
| Bst2BI | CACGAG | 2 cut(s) | 974, 1160 |
| Bst4CI | ACNGT | 5 cut(s) | 398, 595, 1434, 1498, 2015 |
| Bst6I | CTCTTC | 1 cut(s) | 1012 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1171 |
| BstAUI | TGTACA | 2 cut(s) | 467, 1690 |
| BstC8I | GCNNGC | 2 cut(s) | 359, 1270 |
| BstDEI | CTNAG | 8 cut(s) | 402, 576, 618, 704, 1194, 1243, 1745, 1783 |
| BstDSI | CCRYGG | 1 cut(s) | 1430 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1153 |
| BstF5I | GGATG | 4 cut(s) | 119, 743, 919, 1040 |
| BstFNI | CGCG | 1 cut(s) | 125 |
| BstH2I | RGCGCY | 1 cut(s) | 305 |
| BstHHI | GCGC | 2 cut(s) | 304, 919 |
| BstKTI | GATC | 5 cut(s) | 184, 692, 829, 1837, 2054 |
| BstMAI | GTCTC | 3 cut(s) | 138, 849, 944 |
| BstMBI | GATC | 5 cut(s) | 181, 689, 826, 1834, 2051 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 131, 137, 363, 1171, 1779 |
| BstNSI | RCATGY | 2 cut(s) | 796, 1262 |
| BstSCI | CCNGG | 2 cut(s) | 777, 778 |
| BstSFI | CTRYAG | 3 cut(s) | 339, 384, 1497 |
| BstSLI | GKGCMC | 1 cut(s) | 785 |
| BstUI | CGCG | 1 cut(s) | 125 |
| BstV1I | GCAGC | 3 cut(s) | 118, 1306, 1772 |
| BstV2I | GAAGAC | 4 cut(s) | 660, 1092, 1389, 1470 |
| BstX2I | RGATCY | 1 cut(s) | 1834 |
| BstYI | RGATCY | 1 cut(s) | 1834 |
| BsuI | GTATCC | 1 cut(s) | 577 |
| BsuRI | GGCC | 3 cut(s) | 511, 742, 1449 |
| BtgI | CCRYGG | 1 cut(s) | 1430 |
| BtsCI | GGATG | 4 cut(s) | 119, 743, 919, 1040 |
| BtsI | GCAGTG | 1 cut(s) | 1955 |
| BtsIMutI | CAGTG | 3 cut(s) | 1494, 1558, 1955 |
| BtuMI | TCGCGA | 1 cut(s) | 125 |
| BveI | ACCTGC | 2 cut(s) | 485, 1359 |
| Cac8I | GCNNGC | 2 cut(s) | 359, 1270 |
| CaiI | CAGNNNCTG | 2 cut(s) | 593, 1744 |
| CciI | TCATGA | 1 cut(s) | 1129 |
| CfoI | GCGC | 2 cut(s) | 304, 919 |
| Cfr13I | GGNCC | 1 cut(s) | 1463 |
| Cfr9I | CCCGGG | 1 cut(s) | 778 |
| Csp6I | GTAC | 4 cut(s) | 468, 897, 1691, 1742 |
| CviQI | GTAC | 4 cut(s) | 468, 897, 1691, 1742 |
| DdeI | CTNAG | 8 cut(s) | 402, 576, 618, 704, 1194, 1243, 1745, 1783 |
| DpnI | GATC | 5 cut(s) | 183, 691, 828, 1836, 2053 |
| DpnII | GATC | 5 cut(s) | 181, 689, 826, 1834, 2051 |
| Eam1104I | CTCTTC | 1 cut(s) | 1012 |
| EarI | CTCTTC | 1 cut(s) | 1012 |
| EciI | GGCGGA | 1 cut(s) | 596 |
| Ecl136II | GAGCTC | 2 cut(s) | 624, 680 |
| Eco130I | CCWWGG | 2 cut(s) | 367, 506 |
| Eco24I | GRGCYC | 3 cut(s) | 121, 626, 682 |
| Eco47I | GGWCC | 1 cut(s) | 1463 |
| Eco53kI | GAGCTC | 2 cut(s) | 624, 680 |
| Eco57I | CTGAAG | 4 cut(s) | 156, 680, 993, 1356 |
| Eco88I | CYCGRG | 1 cut(s) | 778 |
| EcoICRI | GAGCTC | 2 cut(s) | 624, 680 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1153 |
| EcoRI | GAATTC | 1 cut(s) | 1056 |
| EcoT14I | CCWWGG | 2 cut(s) | 367, 506 |
| EcoT38I | GRGCYC | 3 cut(s) | 121, 626, 682 |
| ErhI | CCWWGG | 2 cut(s) | 367, 506 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 679, 711, 856, 888 |
| FaqI | GGGAC | 1 cut(s) | 1476 |
| FbaI | TGATCA | 1 cut(s) | 181 |
| FblI | GTMKAC | 2 cut(s) | 258, 1137 |
| Fnu4HI | GCNGC | 3 cut(s) | 132, 1320, 1761 |
| FokI | GGATG | 4 cut(s) | 126, 750, 926, 1047 |
| FriOI | GRGCYC | 3 cut(s) | 121, 626, 682 |
| Fsp4HI | GCNGC | 3 cut(s) | 132, 1320, 1761 |
| FspBI | CTAG | 7 cut(s) | 305, 491, 543, 633, 1424, 1676, 1788 |
| GlaI | GCGC | 2 cut(s) | 303, 918 |
| GluI | GCNGC | 3 cut(s) | 132, 1320, 1761 |
| HaeII | RGCGCY | 1 cut(s) | 305 |
| HaeIII | GGCC | 3 cut(s) | 511, 742, 1449 |
| HapII | CCGG | 2 cut(s) | 141, 779 |
| HhaI | GCGC | 2 cut(s) | 304, 919 |
| Hin6I | GCGC | 2 cut(s) | 302, 917 |
| HinP1I | GCGC | 2 cut(s) | 302, 917 |
| HincII | GTYRAC | 3 cut(s) | 720, 1138, 1969 |
| HindII | GTYRAC | 3 cut(s) | 720, 1138, 1969 |
| HindIII | AAGCTT | 3 cut(s) | 1088, 1240, 2059 |
| HpaII | CCGG | 2 cut(s) | 141, 779 |
| HphI | GGTGA | 3 cut(s) | 509, 581, 823 |
| Hpy166II | GTNNAC | 7 cut(s) | 259, 457, 720, 770, 1138, 1858, 1969 |
| Hpy188III | TCNNGA | 8 cut(s) | 31, 124, 892, 943, 974, 1130, 1605, 1838 |
| Hpy8I | GTNNAC | 7 cut(s) | 259, 457, 720, 770, 1138, 1858, 1969 |
| HpyAV | CCTTC | 4 cut(s) | 187, 227, 875, 1111 |
| HpyCH4III | ACNGT | 5 cut(s) | 398, 595, 1434, 1498, 2015 |
| HpyCH4IV | ACGT | 2 cut(s) | 1475, 1850 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 131, 137, 363, 1171, 1779 |
| HpyF3I | CTNAG | 8 cut(s) | 402, 576, 618, 704, 1194, 1243, 1745, 1783 |
| HpySE526I | ACGT | 2 cut(s) | 1475, 1850 |
| HspAI | GCGC | 2 cut(s) | 302, 917 |
| Ksp22I | TGATCA | 1 cut(s) | 181 |
| Kzo9I | GATC | 5 cut(s) | 181, 689, 826, 1834, 2051 |
| LmnI | GCTCC | 4 cut(s) | 629, 1827, 2002, 2046 |
| Lsp1109I | GCAGC | 3 cut(s) | 118, 1306, 1772 |
| LweI | GCATC | 4 cut(s) | 904, 1025, 1255, 1782 |
| MaeI | CTAG | 7 cut(s) | 305, 491, 543, 633, 1424, 1676, 1788 |
| MaeII | ACGT | 2 cut(s) | 1475, 1850 |
| MaeIII | GTNAC | 5 cut(s) | 569, 644, 971, 1553, 1938 |
| MalI | GATC | 5 cut(s) | 183, 691, 828, 1836, 2053 |
| MboI | GATC | 5 cut(s) | 181, 689, 826, 1834, 2051 |
| MflI | RGATCY | 1 cut(s) | 1834 |
| MhlI | GDGCHC | 5 cut(s) | 121, 626, 682, 785, 1167 |
| MlyI | GAGTC | 5 cut(s) | 529, 566, 725, 946, 1816 |
| MseI | TTAA | 4 cut(s) | 81, 375, 597, 932 |
| MslI | CAYNNNNRTG | 5 cut(s) | 425, 797, 1263, 1386, 1499 |
| MspI | CCGG | 2 cut(s) | 141, 779 |
| MspR9I | CCNGG | 2 cut(s) | 779, 780 |
| Mva1269I | GAATGC | 1 cut(s) | 1496 |
| MvnI | CGCG | 1 cut(s) | 125 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 131, 137, 363, 1171, 1779 |
| NciI | CCSGG | 2 cut(s) | 779, 780 |
| NdeII | GATC | 5 cut(s) | 181, 689, 826, 1834, 2051 |
| NlaIV | GGNNCC | 2 cut(s) | 1464, 1836 |
| NmuCI | GTSAC | 4 cut(s) | 569, 644, 971, 1553 |
| NruI | TCGCGA | 1 cut(s) | 125 |
| NspI | RCATGY | 2 cut(s) | 796, 1262 |
| OliI | CACNNNNGTG | 2 cut(s) | 425, 1499 |
| PagI | TCATGA | 1 cut(s) | 1129 |
| PaqCI | CACCTGC | 1 cut(s) | 485 |
| PciI | ACATGT | 2 cut(s) | 792, 1258 |
| PctI | GAATGC | 1 cut(s) | 1496 |
| PfeI | GAWTC | 6 cut(s) | 380, 1185, 1311, 1705, 1735, 1883 |
| PkrI | GCNGC | 3 cut(s) | 133, 1321, 1762 |
| PleI | GAGTC | 5 cut(s) | 528, 566, 724, 945, 1815 |
| PpsI | GAGTC | 5 cut(s) | 528, 566, 724, 945, 1815 |
| PscI | ACATGT | 2 cut(s) | 792, 1258 |
| PsiI | TTATAA | 1 cut(s) | 531 |
| Psp124BI | GAGCTC | 2 cut(s) | 626, 682 |
| PspN4I | GGNNCC | 2 cut(s) | 1464, 1836 |
| PspPI | GGNCC | 1 cut(s) | 1463 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 742, 774 |
| PstNI | CAGNNNCTG | 2 cut(s) | 593, 1744 |
| PsuI | RGATCY | 1 cut(s) | 1834 |
| RruI | TCGCGA | 1 cut(s) | 125 |
| RsaI | GTAC | 4 cut(s) | 469, 898, 1692, 1743 |
| RsaNI | GTAC | 4 cut(s) | 468, 897, 1691, 1742 |
| RseI | CAYNNNNRTG | 5 cut(s) | 425, 797, 1263, 1386, 1499 |
| SacI | GAGCTC | 2 cut(s) | 626, 682 |
| SalI | GTCGAC | 1 cut(s) | 1136 |
| SaqAI | TTAA | 4 cut(s) | 81, 375, 597, 932 |
| SatI | GCNGC | 3 cut(s) | 132, 1320, 1761 |
| Sau3AI | GATC | 5 cut(s) | 181, 689, 826, 1834, 2051 |
| Sau96I | GGNCC | 1 cut(s) | 1463 |
| ScaI | AGTACT | 1 cut(s) | 898 |
| SchI | GAGTC | 5 cut(s) | 529, 566, 725, 946, 1816 |
| ScrFI | CCNGG | 2 cut(s) | 779, 780 |
| SduI | GDGCHC | 5 cut(s) | 121, 626, 682, 785, 1167 |
| SfaNI | GCATC | 4 cut(s) | 904, 1025, 1255, 1782 |
| SfcI | CTRYAG | 3 cut(s) | 339, 384, 1497 |
| SinI | GGWCC | 1 cut(s) | 1463 |
| SmaI | CCCGGG | 1 cut(s) | 780 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 425, 797, 1263, 1386, 1499 |
| SmlI | CTYRAG | 1 cut(s) | 8 |
| SmoI | CTYRAG | 1 cut(s) | 8 |
| SsiI | CCGC | 2 cut(s) | 158, 581 |
| SspI | AATATT | 2 cut(s) | 1532, 2030 |
| SspMI | CTAG | 7 cut(s) | 305, 491, 543, 633, 1424, 1676, 1788 |
| SstI | GAGCTC | 2 cut(s) | 626, 682 |
| StyD4I | CCNGG | 2 cut(s) | 777, 778 |
| StyI | CCWWGG | 2 cut(s) | 367, 506 |
| TaaI | ACNGT | 5 cut(s) | 398, 595, 1434, 1498, 2015 |
| TaiI | ACGT | 2 cut(s) | 1478, 1853 |
| TaqI | TCGA | 3 cut(s) | 1137, 1716, 1878 |
| TatI | WGTACW | 3 cut(s) | 467, 896, 1690 |
| TfiI | GAWTC | 6 cut(s) | 380, 1185, 1311, 1705, 1735, 1883 |
| Tru1I | TTAA | 4 cut(s) | 81, 375, 597, 932 |
| Tru9I | TTAA | 4 cut(s) | 81, 375, 597, 932 |
| TscAI | CASTG | 3 cut(s) | 1501, 1558, 1962 |
| TseFI | GTSAC | 4 cut(s) | 569, 644, 971, 1553 |
| TseI | GCWGC | 3 cut(s) | 131, 1319, 1760 |
| Tsp45I | GTSAC | 4 cut(s) | 569, 644, 971, 1553 |
| TspDTI | ATGAA | 9 cut(s) | 152, 342, 492, 666, 1109, 1146, 1370, 1525, 1926 |
| TspGWI | ACGGA | 1 cut(s) | 178 |
| TspMI | CCCGGG | 1 cut(s) | 778 |
| TspRI | CASTG | 3 cut(s) | 1501, 1558, 1962 |
| VpaK11BI | GGWCC | 1 cut(s) | 1463 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1153 |
| XapI | RAATTY | 5 cut(s) | 563, 1000, 1056, 1252, 1711 |
| XceI | RCATGY | 2 cut(s) | 796, 1262 |
| XmaI | CCCGGG | 1 cut(s) | 778 |
| XmiI | GTMKAC | 2 cut(s) | 258, 1137 |
| XspI | CTAG | 7 cut(s) | 305, 491, 543, 633, 1424, 1676, 1788 |
| ZrmI | AGTACT | 1 cut(s) | 898 |