MD15G1244700.v1.1

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005017
Physical Location & Seq
Reverse (-)
20185092 .. 20185559
468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1244700.v1.1.491

Sequence Viewer

Length: 468 bp
ATGCTTTCTGCCTCGTCCTCCACCGCATTCCTCGCCGCTCCAATTCTCTCCGCCACAGCCTCTCCTAAAACGTCGACACGTGTACGATTTCAGCCGATTCGCGCCTTCGCCACCGGCTCCGCTTACGCTTTGCCTGAGGCCGAGACGGCGTCGTATCTCCGCCCTCAAACAATGACCTCGCTGTACGAAATCCTCGGCATCCCAGCCGTCGCTACGTGTCAGGAGATTAAATCGGCGTATCGGAGACTGGCTCGGGTTTGCCACCCCGACGTGGCGGCGATCGAGCGCAAGGACACGTCGGCCGACGAGTTCATGAAGATTCACGCTGCCTACTCCACACTGTCGGATCCCGACAAACGCGCCGATTACGACCGGCAATTTATGCGGCGGAGCCGGCCGTTGTCGTCGGCGTCAAGATATTCCGGGTACTACACTCGCCGGAACTGGGAGACCGACCAGTGCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

156

Amino Acids

17.39

Weight (kDa)

9.4

Isoelectric Point (pI)

52.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 61 - 124 1.7e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 74
AccII CGCG 2 cut(s) 102, 360
AciI CCGC 8 cut(s) 24, 36, 51, 120, 160, 275, 385, 388
AclWI GGATC 2 cut(s) 341, 354
AcoI YGGCCR 2 cut(s) 300, 395
AcvI CACGTG 1 cut(s) 80
AcyI GRCGYC 2 cut(s) 149, 410
AfaI GTAC 3 cut(s) 84, 185, 428
AfiI CCNNNNNNNGG 2 cut(s) 271, 445
AflIII ACRYGT 4 cut(s) 77, 79, 215, 294
AjiI CACGTC 2 cut(s) 271, 297
Alw26I GTCTC 3 cut(s) 137, 238, 443
AlwI GGATC 2 cut(s) 341, 354
Ama87I CYCGRG 1 cut(s) 252
AoxI GGCC 3 cut(s) 138, 300, 395
ApeKI GCWGC 1 cut(s) 326
AspLEI GCGC 3 cut(s) 104, 288, 362
AsuC2I CCSGG 1 cut(s) 424
AvaI CYCGRG 1 cut(s) 252
AxyI CCTNAGG 1 cut(s) 135
BamHI GGATCC 1 cut(s) 346
BbrPI CACGTG 1 cut(s) 80
BbvI GCAGC 1 cut(s) 313
BceAI ACGGC 3 cut(s) 162, 191, 382
BcnI CCSGG 1 cut(s) 424
BcoDI GTCTC 3 cut(s) 137, 238, 443
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 4 cut(s) 36, 276, 327, 386
BlsI GCNGC 4 cut(s) 37, 277, 328, 387
Bme1390I CCNGG 1 cut(s) 424
BmeT110I CYCGRG 1 cut(s) 252
BmgBI CACGTC 2 cut(s) 271, 297
BmiI GGNNCC 3 cut(s) 118, 348, 392
BmrFI CCNGG 1 cut(s) 424
BmrI ACTGGG 1 cut(s) 454
BmsI GCATC 1 cut(s) 207
BmuI ACTGGG 1 cut(s) 454
BplI GAGNNNNNCTC 2 cut(s) 235, 267
BpuMI CCSGG 1 cut(s) 424
BsaAI YACGTR 2 cut(s) 80, 216
BsaHI GRCGYC 2 cut(s) 149, 410
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 193
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 445
Bse118I RCCGGY 3 cut(s) 113, 372, 393
Bse1I ACTGG 3 cut(s) 252, 449, 457
Bse21I CCTNAGG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 2 cut(s) 271, 445
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 3 cut(s) 252, 449, 457
BseX3I CGGCCG 2 cut(s) 300, 395
BseXI GCAGC 1 cut(s) 313
BseYI CCCAGC 1 cut(s) 202
Bsh1236I CGCG 2 cut(s) 102, 360
Bsh1285I CGRYCG 4 cut(s) 282, 303, 373, 398
BshFI GGCC 3 cut(s) 140, 302, 397
BsiEI CGRYCG 4 cut(s) 282, 303, 373, 398
BsiHKCI CYCGRG 1 cut(s) 252
BsiSI CCGG 5 cut(s) 114, 373, 394, 423, 439
BslI CCNNNNNNNGG 2 cut(s) 271, 445
BsmAI GTCTC 3 cut(s) 137, 238, 443
BsmBI CGTCTC 1 cut(s) 137
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 3 cut(s) 140, 302, 397
Bso31I GGTCTC 1 cut(s) 443
BsoBI CYCGRG 1 cut(s) 252
Bsp143I GATC 2 cut(s) 279, 346
BspACI CCGC 8 cut(s) 24, 36, 51, 120, 160, 275, 385, 388
BspANI GGCC 3 cut(s) 140, 302, 397
BspCNI CTCAG 1 cut(s) 127
BspFNI CGCG 2 cut(s) 102, 360
BspHI TCATGA 1 cut(s) 312
BspLI GGNNCC 3 cut(s) 118, 348, 392
BspPI GGATC 2 cut(s) 341, 354
BspTNI GGTCTC 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 38
BsrFI RCCGGY 3 cut(s) 113, 372, 393
BsrI ACTGG 3 cut(s) 252, 449, 457
BssAI RCCGGY 3 cut(s) 113, 372, 393
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 2 cut(s) 279, 346
BssNI GRCGYC 2 cut(s) 149, 410
Bst4CI ACNGT 1 cut(s) 342
BstACI GRCGYC 2 cut(s) 149, 410
BstAPI GCANNNNNTGC 1 cut(s) 382
BstBAI YACGTR 2 cut(s) 80, 216
BstC8I GCNNGC 1 cut(s) 395
BstDEI CTNAG 1 cut(s) 135
BstF5I GGATG 1 cut(s) 198
BstFNI CGCG 2 cut(s) 102, 360
BstHHI GCGC 3 cut(s) 104, 288, 362
BstKTI GATC 2 cut(s) 282, 349
BstMAI GTCTC 3 cut(s) 137, 238, 443
BstMBI GATC 2 cut(s) 279, 346
BstMCI CGRYCG 4 cut(s) 282, 303, 373, 398
BstMWI GCNNNNNNNGC 4 cut(s) 32, 146, 382, 394
BstSCI CCNGG 1 cut(s) 422
BstUI CGCG 2 cut(s) 102, 360
BstV1I GCAGC 1 cut(s) 313
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BstZI CGGCCG 2 cut(s) 300, 395
Bsu36I CCTNAGG 1 cut(s) 135
BsuRI GGCC 3 cut(s) 140, 302, 397
BtrI CACGTC 2 cut(s) 271, 297
BtsCI GGATG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 338, 464
Cac8I GCNNGC 1 cut(s) 395
CciI TCATGA 1 cut(s) 312
CfoI GCGC 3 cut(s) 104, 288, 362
Cfr10I RCCGGY 3 cut(s) 113, 372, 393
CseI GACGC 2 cut(s) 138, 399
Csp6I GTAC 3 cut(s) 83, 184, 427
CviAII CATG 1 cut(s) 313
CviJI RGCY 9 cut(s) 59, 94, 117, 140, 206, 251, 302, 393, 397
CviKI_1 RGCY 9 cut(s) 59, 94, 117, 140, 206, 251, 302, 393, 397
CviQI GTAC 3 cut(s) 83, 184, 427
DdeI CTNAG 1 cut(s) 135
DpnI GATC 2 cut(s) 281, 348
DpnII GATC 2 cut(s) 279, 346
EaeI YGGCCR 2 cut(s) 300, 395
EagI CGGCCG 2 cut(s) 300, 395
EciI GGCGGA 3 cut(s) 40, 149, 403
EclXI CGGCCG 2 cut(s) 300, 395
Eco31I GGTCTC 1 cut(s) 443
Eco52I CGGCCG 2 cut(s) 300, 395
Eco72I CACGTG 1 cut(s) 80
Eco81I CCTNAGG 1 cut(s) 135
Eco88I CYCGRG 1 cut(s) 252
Esp3I CGTCTC 1 cut(s) 137
FaeI CATG 1 cut(s) 316
FaiI YATR 2 cut(s) 314, 383
FatI CATG 1 cut(s) 312
FblI GTMKAC 1 cut(s) 74
Fnu4HI GCNGC 4 cut(s) 36, 276, 327, 386
FokI GGATG 1 cut(s) 185
Fsp4HI GCNGC 4 cut(s) 36, 276, 327, 386
GlaI GCGC 3 cut(s) 103, 287, 361
GluI GCNGC 4 cut(s) 36, 276, 327, 386
GsaI CCCAGC 1 cut(s) 206
HaeIII GGCC 3 cut(s) 140, 302, 397
HapII CCGG 5 cut(s) 114, 373, 394, 423, 439
HgaI GACGC 2 cut(s) 138, 399
HhaI GCGC 3 cut(s) 104, 288, 362
Hin1I GRCGYC 2 cut(s) 149, 410
Hin1II CATG 1 cut(s) 316
Hin6I GCGC 3 cut(s) 102, 286, 360
HinP1I GCGC 3 cut(s) 102, 286, 360
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HinfI GANTC 2 cut(s) 97, 319
HpaII CCGG 5 cut(s) 114, 373, 394, 423, 439
Hpy166II GTNNAC 2 cut(s) 75, 83
Hpy188I TCNGA 2 cut(s) 243, 346
Hpy188III TCNNGA 4 cut(s) 221, 313, 350, 414
Hpy8I GTNNAC 2 cut(s) 75, 83
Hpy99I CGWCG 7 cut(s) 76, 154, 212, 272, 301, 308, 409
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 5 cut(s) 71, 79, 215, 270, 296
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 146, 382, 394
HpyF3I CTNAG 1 cut(s) 135
HpySE526I ACGT 5 cut(s) 71, 79, 215, 270, 296
Hsp92I GRCGYC 2 cut(s) 149, 410
Hsp92II CATG 1 cut(s) 316
HspAI GCGC 3 cut(s) 102, 286, 360
KroI GCCGGC 1 cut(s) 393
KroNI GCCGGC 1 cut(s) 395
Kzo9I GATC 2 cut(s) 279, 346
LmnI GCTCC 3 cut(s) 43, 122, 390
Lsp1109I GCAGC 1 cut(s) 313
LweI GCATC 1 cut(s) 207
MaeII ACGT 5 cut(s) 71, 79, 215, 270, 296
MalI GATC 2 cut(s) 281, 348
MbiI CCGCTC 1 cut(s) 38
MboI GATC 2 cut(s) 279, 346
MboII GAAGA 1 cut(s) 328
MflI RGATCY 1 cut(s) 346
MluCI AATT 2 cut(s) 42, 377
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 8 cut(s) 22, 28, 41, 70, 130, 174, 187, 203
MroNI GCCGGC 1 cut(s) 393
MseI TTAA 1 cut(s) 228
MspI CCGG 5 cut(s) 114, 373, 394, 423, 439
MspR9I CCNGG 1 cut(s) 424
Mva1269I GAATGC 1 cut(s) 26
MvnI CGCG 2 cut(s) 102, 360
MwoI GCNNNNNNNGC 4 cut(s) 32, 146, 382, 394
NaeI GCCGGC 1 cut(s) 395
NciI CCSGG 1 cut(s) 424
NdeII GATC 2 cut(s) 279, 346
NgoMIV GCCGGC 1 cut(s) 393
NlaIII CATG 1 cut(s) 316
NlaIV GGNNCC 3 cut(s) 118, 348, 392
NmeAIII GCCGAG 2 cut(s) 166, 174
PagI TCATGA 1 cut(s) 312
PctI GAATGC 1 cut(s) 26
PdiI GCCGGC 1 cut(s) 395
PfeI GAWTC 2 cut(s) 97, 319
PflFI GACNNNGTC 1 cut(s) 148
PkrI GCNGC 4 cut(s) 37, 277, 328, 387
Ple19I CGATCG 1 cut(s) 282
PmaCI CACGTG 1 cut(s) 80
PmlI CACGTG 1 cut(s) 80
Ppu21I YACGTR 2 cut(s) 80, 216
PspCI CACGTG 1 cut(s) 80
PspFI CCCAGC 1 cut(s) 202
PspN4I GGNNCC 3 cut(s) 118, 348, 392
PsuI RGATCY 1 cut(s) 346
PsyI GACNNNGTC 1 cut(s) 148
PvuI CGATCG 1 cut(s) 282
RsaI GTAC 3 cut(s) 84, 185, 428
RsaNI GTAC 3 cut(s) 83, 184, 427
SalI GTCGAC 1 cut(s) 73
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 4 cut(s) 36, 276, 327, 386
Sau3AI GATC 2 cut(s) 279, 346
ScrFI CCNGG 1 cut(s) 424
SetI ASST 6 cut(s) 74, 82, 179, 218, 273, 299
SfaNI GCATC 1 cut(s) 207
Sse9I AATT 2 cut(s) 42, 377
SsiI CCGC 8 cut(s) 24, 36, 51, 120, 160, 275, 385, 388
StyD4I CCNGG 1 cut(s) 422
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 5 cut(s) 74, 82, 218, 273, 299
TaqI TCGA 2 cut(s) 74, 282
TaqII GACCGA 1 cut(s) 467
TasI AATT 2 cut(s) 42, 377
TauI GCSGC 3 cut(s) 38, 278, 388
TfiI GAWTC 2 cut(s) 97, 319
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 2 cut(s) 345, 464
TseI GCWGC 1 cut(s) 326
TspDTI ATGAA 2 cut(s) 301, 329
TspRI CASTG 2 cut(s) 345, 464
Tth111I GACNNNGTC 1 cut(s) 148
XmiI GTMKAC 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.