pycom02g10270

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005017
Physical Location & Seq
Reverse (-)
7140801 .. 7141265
465 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g10270.1

Sequence Viewer

Length: 465 bp
ATGCTTTCTGCTTCATTCTCCACCACATTCCTCACCGCTCCGATTCTCTCCGCCACAGCCTCTCCAAAAACGTCGACACGTGTACGATTTCAGCCGATTCGCGCCTTCGCCTCCGGCTCCGCTTACGCTTCTGCTGAGGCCGAGACGCCGTCGTATCTCCGCCCTCAAACCATGACCTCGCTGTACGAAATCCTCGGCATACCGGCCGTCGCCACGTGTCAGGAGATCAAATCGGCGTACCGAAAACTGGTTCGGGTTTGCCACCCGGACGTGGCGGCGATCGAGCGCAAGGACACGTCGGCCGACGAGTTCATGAAGATTCACGCTGCCTACTCCACGCTATCAGATCCCCAAAAGCGCGCCGATTACGACCGGCAATTTATGCGGCGTAGCCGCCCGTTGGCTTCGGCGTCGGGATATTCGGGGTACTACACTCGCCGGAACTGGGAGACCGATCGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

155

Amino Acids

17.2

Weight (kDa)

9.61

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 61 - 124 1.6e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 74
AccII CGCG 2 cut(s) 102, 360
AciI CCGC 7 cut(s) 36, 51, 120, 160, 275, 385, 394
AclWI GGATC 1 cut(s) 341
AcoI YGGCCR 2 cut(s) 204, 300
AcvI CACGTG 2 cut(s) 80, 216
AcyI GRCGYC 2 cut(s) 146, 410
AfaI GTAC 4 cut(s) 84, 185, 239, 428
AfiI CCNNNNNNNGG 4 cut(s) 247, 271, 400, 445
AflIII ACRYGT 4 cut(s) 77, 79, 215, 294
AjiI CACGTC 2 cut(s) 271, 297
Alw26I GTCTC 2 cut(s) 137, 443
AlwI GGATC 1 cut(s) 341
AoxI GGCC 3 cut(s) 138, 204, 300
ApeKI GCWGC 1 cut(s) 326
AspLEI GCGC 4 cut(s) 104, 288, 360, 362
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 25
BbrPI CACGTG 2 cut(s) 80, 216
BbvCI CCTCAGC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 313
BceAI ACGGC 2 cut(s) 133, 191
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 2 cut(s) 137, 443
BisI GCNGC 4 cut(s) 276, 327, 386, 394
BlsI GCNGC 4 cut(s) 277, 328, 387, 395
Bme1390I CCNGG 1 cut(s) 266
BmgBI CACGTC 2 cut(s) 271, 297
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 1 cut(s) 266
BmrI ACTGGG 1 cut(s) 454
BmuI ACTGGG 1 cut(s) 454
Bpu10I CCTNAGC 1 cut(s) 135
BpuMI CCSGG 1 cut(s) 266
Bsa29I ATCGAT 1 cut(s) 457
BsaAI YACGTR 2 cut(s) 80, 216
BsaHI GRCGYC 2 cut(s) 146, 410
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 193
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 271, 400, 445
Bse118I RCCGGY 2 cut(s) 202, 372
Bse1I ACTGG 2 cut(s) 252, 449
BseCI ATCGAT 1 cut(s) 457
BseDI CCNNGG 1 cut(s) 193
BseLI CCNNNNNNNGG 4 cut(s) 247, 271, 400, 445
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 2 cut(s) 252, 449
BsePI GCGCGC 1 cut(s) 358
BseX3I CGGCCG 2 cut(s) 204, 300
BseXI GCAGC 1 cut(s) 313
Bsh1236I CGCG 2 cut(s) 102, 360
Bsh1285I CGRYCG 5 cut(s) 207, 282, 303, 373, 457
BshFI GGCC 3 cut(s) 140, 206, 302
BshVI ATCGAT 1 cut(s) 457
BsiEI CGRYCG 5 cut(s) 207, 282, 303, 373, 457
BsiSI CCGG 5 cut(s) 114, 203, 266, 373, 439
BslI CCNNNNNNNGG 4 cut(s) 247, 271, 400, 445
BsmAI GTCTC 2 cut(s) 137, 443
BsmBI CGTCTC 1 cut(s) 137
BsnI GGCC 3 cut(s) 140, 206, 302
Bso31I GGTCTC 1 cut(s) 443
Bsp143I GATC 5 cut(s) 225, 279, 346, 454, 458
BspACI CCGC 7 cut(s) 36, 51, 120, 160, 275, 385, 394
BspANI GGCC 3 cut(s) 140, 206, 302
BspCNI CTCAG 1 cut(s) 127
BspDI ATCGAT 1 cut(s) 457
BspFNI CGCG 2 cut(s) 102, 360
BspHI TCATGA 1 cut(s) 312
BspLI GGNNCC 1 cut(s) 118
BspPI GGATC 1 cut(s) 341
BspTNI GGTCTC 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 38
BsrFI RCCGGY 2 cut(s) 202, 372
BsrI ACTGG 2 cut(s) 252, 449
BssAI RCCGGY 2 cut(s) 202, 372
BssECI CCNNGG 1 cut(s) 193
BssHII GCGCGC 1 cut(s) 358
BssMI GATC 5 cut(s) 225, 279, 346, 454, 458
BssNI GRCGYC 2 cut(s) 146, 410
BstACI GRCGYC 2 cut(s) 146, 410
BstAPI GCANNNNNTGC 1 cut(s) 382
BstBAI YACGTR 2 cut(s) 80, 216
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 1 cut(s) 135
BstFNI CGCG 2 cut(s) 102, 360
BstHHI GCGC 4 cut(s) 104, 288, 360, 362
BstKTI GATC 5 cut(s) 228, 282, 349, 457, 461
BstMAI GTCTC 2 cut(s) 137, 443
BstMBI GATC 5 cut(s) 225, 279, 346, 454, 458
BstMCI CGRYCG 5 cut(s) 207, 282, 303, 373, 457
BstMWI GCNNNNNNNGC 1 cut(s) 382
BstSCI CCNGG 1 cut(s) 264
BstUI CGCG 2 cut(s) 102, 360
BstV1I GCAGC 1 cut(s) 313
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BstZI CGGCCG 2 cut(s) 204, 300
Bsu15I ATCGAT 1 cut(s) 457
BsuRI GGCC 3 cut(s) 140, 206, 302
BsuTUI ATCGAT 1 cut(s) 457
BtrI CACGTC 2 cut(s) 271, 297
Cac8I GCNNGC 1 cut(s) 360
CciI TCATGA 1 cut(s) 312
CfoI GCGC 4 cut(s) 104, 288, 360, 362
Cfr10I RCCGGY 2 cut(s) 202, 372
ClaI ATCGAT 1 cut(s) 457
CseI GACGC 2 cut(s) 154, 399
Csp6I GTAC 4 cut(s) 83, 184, 238, 427
CviAII CATG 2 cut(s) 172, 313
CviJI RGCY 8 cut(s) 59, 94, 117, 140, 206, 302, 393, 404
CviKI_1 RGCY 8 cut(s) 59, 94, 117, 140, 206, 302, 393, 404
CviQI GTAC 4 cut(s) 83, 184, 238, 427
DdeI CTNAG 1 cut(s) 135
DpnI GATC 5 cut(s) 227, 281, 348, 456, 460
DpnII GATC 5 cut(s) 225, 279, 346, 454, 458
EaeI YGGCCR 2 cut(s) 204, 300
EagI CGGCCG 2 cut(s) 204, 300
EciI GGCGGA 2 cut(s) 40, 149
EclXI CGGCCG 2 cut(s) 204, 300
Eco31I GGTCTC 1 cut(s) 443
Eco52I CGGCCG 2 cut(s) 204, 300
Eco72I CACGTG 2 cut(s) 80, 216
Esp3I CGTCTC 1 cut(s) 137
FaeI CATG 2 cut(s) 175, 316
FaiI YATR 5 cut(s) 173, 200, 314, 383, 463
FatI CATG 2 cut(s) 171, 312
FblI GTMKAC 1 cut(s) 74
Fnu4HI GCNGC 4 cut(s) 276, 327, 386, 394
Fsp4HI GCNGC 4 cut(s) 276, 327, 386, 394
GlaI GCGC 4 cut(s) 103, 287, 359, 361
GluI GCNGC 4 cut(s) 276, 327, 386, 394
HaeIII GGCC 3 cut(s) 140, 206, 302
HapII CCGG 5 cut(s) 114, 203, 266, 373, 439
HgaI GACGC 2 cut(s) 154, 399
HhaI GCGC 4 cut(s) 104, 288, 360, 362
Hin1I GRCGYC 2 cut(s) 146, 410
Hin1II CATG 2 cut(s) 175, 316
Hin6I GCGC 4 cut(s) 102, 286, 358, 360
HinP1I GCGC 4 cut(s) 102, 286, 358, 360
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HinfI GANTC 3 cut(s) 43, 97, 319
HpaII CCGG 5 cut(s) 114, 203, 266, 373, 439
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 2 cut(s) 75, 83
Hpy188I TCNGA 2 cut(s) 42, 346
Hpy188III TCNNGA 3 cut(s) 221, 313, 414
Hpy8I GTNNAC 2 cut(s) 75, 83
Hpy99I CGWCG 6 cut(s) 76, 154, 212, 301, 308, 415
HpyAV CCTTC 1 cut(s) 115
HpyCH4IV ACGT 5 cut(s) 71, 79, 215, 270, 296
HpyF10VI GCNNNNNNNGC 1 cut(s) 382
HpyF3I CTNAG 1 cut(s) 135
HpySE526I ACGT 5 cut(s) 71, 79, 215, 270, 296
Hsp92I GRCGYC 2 cut(s) 146, 410
Hsp92II CATG 2 cut(s) 175, 316
HspAI GCGC 4 cut(s) 102, 286, 358, 360
Kzo9I GATC 5 cut(s) 225, 279, 346, 454, 458
LmnI GCTCC 2 cut(s) 43, 122
LpnPI CCDG 8 cut(s) 127, 206, 216, 233, 279, 386, 430, 452
Lsp1109I GCAGC 1 cut(s) 313
MaeII ACGT 5 cut(s) 71, 79, 215, 270, 296
MalI GATC 5 cut(s) 227, 281, 348, 456, 460
MbiI CCGCTC 1 cut(s) 38
MboI GATC 5 cut(s) 225, 279, 346, 454, 458
MboII GAAGA 1 cut(s) 328
MflI RGATCY 1 cut(s) 346
MluCI AATT 1 cut(s) 377
MnlI CCTC 7 cut(s) 41, 70, 121, 130, 174, 187, 203
MspI CCGG 5 cut(s) 114, 203, 266, 373, 439
MspR9I CCNGG 1 cut(s) 266
MvnI CGCG 2 cut(s) 102, 360
MwoI GCNNNNNNNGC 1 cut(s) 382
NciI CCSGG 1 cut(s) 266
NdeII GATC 5 cut(s) 225, 279, 346, 454, 458
NlaIII CATG 2 cut(s) 175, 316
NlaIV GGNNCC 1 cut(s) 118
NmeAIII GCCGAG 2 cut(s) 166, 174
PagI TCATGA 1 cut(s) 312
PauI GCGCGC 1 cut(s) 358
PfeI GAWTC 3 cut(s) 43, 97, 319
PflFI GACNNNGTC 1 cut(s) 148
PkrI GCNGC 4 cut(s) 277, 328, 387, 395
Ple19I CGATCG 2 cut(s) 282, 457
PmaCI CACGTG 2 cut(s) 80, 216
PmlI CACGTG 2 cut(s) 80, 216
Ppu21I YACGTR 2 cut(s) 80, 216
PspCI CACGTG 2 cut(s) 80, 216
PspN4I GGNNCC 1 cut(s) 118
PsuI RGATCY 1 cut(s) 346
PsyI GACNNNGTC 1 cut(s) 148
PteI GCGCGC 1 cut(s) 358
PvuI CGATCG 2 cut(s) 282, 457
RsaI GTAC 4 cut(s) 84, 185, 239, 428
RsaNI GTAC 4 cut(s) 83, 184, 238, 427
SalI GTCGAC 1 cut(s) 73
SatI GCNGC 4 cut(s) 276, 327, 386, 394
Sau3AI GATC 5 cut(s) 225, 279, 346, 454, 458
ScrFI CCNGG 1 cut(s) 266
SetI ASST 6 cut(s) 74, 82, 179, 218, 273, 299
Sse9I AATT 1 cut(s) 377
SsiI CCGC 7 cut(s) 36, 51, 120, 160, 275, 385, 394
StyD4I CCNGG 1 cut(s) 264
TaiI ACGT 5 cut(s) 74, 82, 218, 273, 299
TaqI TCGA 3 cut(s) 74, 282, 457
TasI AATT 1 cut(s) 377
TauI GCSGC 3 cut(s) 278, 388, 396
TfiI GAWTC 3 cut(s) 43, 97, 319
TseI GCWGC 1 cut(s) 326
TspDTI ATGAA 2 cut(s) 301, 329
Tth111I GACNNNGTC 1 cut(s) 148
XmiI GTMKAC 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.