Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009049

Physical Location & Seq

Genomic Coordinates Forward (+)

32847476 .. 32850407

Gene Structure 2932 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1318200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGCTCCCACCCGACCACTCCAGCTTCAACCTCCTCACCCATTTCATCAATATCCACCCGTCCGATCGCGGTCCCGATTCTGATCCCATCTTCCTCGGAGGCCTGTTCCTGCACCCGAGTAACACCGTCCCTCCTTCCTTCCTTTCCTTGATTTCACCCAATGTCCGTTTGCGTTCCCGTTCGGGATGTTGTTTAAGGTGCCGCGGCCGCAGGACGCTGCGTTTTGAAGGTTTGAAGGGAAGAAGCGGCGGTGGCGGCACGCCGTTGTTTTTGTCTGTGAGTTTGTCGATAAGGGGAAGTGAAGGAGATGGTGGTGAGGAAGAGTATGTTCCGGAATCAGGACAAGTCCTGGGGCAAAATGGGGATAAAAGCTTTTCATCGGAGGAGGCCGCCGTCGTGGCGGTGTTCGAGAAGGGTGAGGCTGAGACTGCACGGAAGGGATCGGGTGCTATGAATACCACCAAGCATCTTTGGTCTGGAGCTGTTGCTGCTATGGTTTCAAGGACGTTCGTTGCTCCTCTTGAGAGGCTAAAGCTGGAATATGTAGTTCGTGGCGAACAGAAGAACCTTATTGAACTCATCAGGACGATTGCAGCTTCTCAAGGGCTGAAGGGATTTTGGAAAGGAAATTTTGTCAATATTCTTCGCACGGCACCCTTTAAGGCTATCAATTTCTGTGCTTATGATACTTACAGAAATCATCTGAGGAAACTGTCTCGGAGTGAGGAGTCCACAAATTACGAGAGGTTTCTTGCTGGTGCTGCGGCTGGAATTACAGCTACCTTGCTCTGCTTACCTATGGACACTATCAGGACAAAGATGGTAGCACCTGGTGGAGAAGCCTTGGGTGGTGTAATCGGTGCTTTCCGCCACATGATCCAAACTGAAGGGTTCTTTTCTCTTTACAAGGGTTTACTACCTTCGATTGTGAGCATGGCACCTTCAGGTGCAGTTTTCTACGGTGTTTATGATATACTGAAATCGGCTTATCTTCATTCACCTGAAGGGAGGAAAAGGATCCAACATATGAAACAAGAAGGTGATAAATTGAATGCTTTGGAACAACTGGAGTTGGGTCCCGTTAGAACATTACTATATGGGGCAATCGCCGGTGCTTGTTCTGAAGCTGCTACATATCCATTTGAAGTTGTGAGGAGACACCTTCAAATGCAAGTTCGAGCAACTAAGTTAAGCGCGCTTGCAACTTGTGTTAAAATTGTCGAGCAAGGAGGTGTGCCTGCTCTTTATGCAGGATTAACACCAAGCCTATTGCAGGTTTTACCATCTGCTGCCATAAGTTATCTTGTATACGAGTTCATGAAGATTTTTCTCAAAGTCGAGTCTACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000295 GO:0003674 GO:0005215 GO:0005346 GO:0005347 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006855 GO:0006862 GO:0006950 GO:0006979 GO:0008150 GO:0008509 GO:0008514 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010941 GO:0015075 GO:0015215 GO:0015216 GO:0015217 GO:0015238 GO:0015605 GO:0015711 GO:0015748 GO:0015865 GO:0015866 GO:0015867 GO:0015868 GO:0015893 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0019866 GO:0022857 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031975 GO:0033554 GO:0034220 GO:0034599 GO:0042221 GO:0042493 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051503 GO:0051592 GO:0051716 GO:0055085 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071277 GO:0071702 GO:0071705 GO:0098656 GO:1901264 GO:1901505 GO:1901679

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mito_carr

Protein Analysis

450

Amino Acids

48.8

Weight (kDa)

9.26

Isoelectric Point (pI)

45.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mito_carr	PF00153	151 - 237	1.2e-19	Mitochondrial carrier protein
Mito_carr	PF00153	244 - 331	1.6e-20	Mitochondrial carrier protein
Mito_carr	PF00153	358 - 445	6.6e-20	Mitochondrial carrier protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009049)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64970 AT5G64970 AT5G64970
fragaria_vesca	FvH4_1g20840
malus_domestica	MD01G1030900.v1.1 MD15G1318200.v1.1
prunus_persica	Prupe.6G187500_v2.0.a1
pyrus_communis	pycom01g05840 pycom15g28540
rosa_chinensis	RchiOBHm_Chr2g0113451
rosa_laevigata	RLG00000018064
rosa_multiflora	Rmu_sc0007415.1_g000018 Rmu_sc0020459.1_g000001
rosa_roxburghii	Rroxscaffold_2G00129230 Rroxscaffold_2G00129930
rosa_rugosa	Rorug02G0187600
rosa_samantha	Rh2AG242100 Rh2BG256000 Rh2CG246300 Rh2DG250000
rosa_wichuraiana	Rw0G018830 Rw2G018890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1264
AccB1I	GGYRCC	3 cut(s)	198, 652, 937
AccI	GTMKAC	2 cut(s)	1308, 1343
AccII	CGCG	3 cut(s)	69, 204, 1196
AccIII	TCCGGA	1 cut(s)	331
AclWI	GGATC	5 cut(s)	77, 448, 871, 1012, 1025
AcoI	YGGCCR	1 cut(s)	205
AcsI	RAATTY	1 cut(s)	628
AcuI	CTGAAG	5 cut(s)	629, 906, 927, 1023, 1143
AdeI	CACNNNGTG	1 cut(s)	833
AfiI	CCNNNNNNNGG	2 cut(s)	338, 1273
AgsI	TTSAA	8 cut(s)	28, 227, 235, 501, 575, 1051, 1145, 1166
AjnI	CCWGG	2 cut(s)	348, 829
AluBI	AGCT	7 cut(s)	24, 372, 482, 535, 596, 779, 1127
AluI	AGCT	7 cut(s)	24, 372, 482, 535, 596, 779, 1127
Alw26I	GTCTC	3 cut(s)	419, 720, 1150
AlwI	GGATC	5 cut(s)	77, 448, 871, 1012, 1025
Ama87I	CYCGRG	1 cut(s)	115
Aor13HI	TCCGGA	1 cut(s)	331
AoxI	GGCC	3 cut(s)	100, 205, 387
ApeKI	GCWGC	6 cut(s)	217, 488, 593, 761, 1127, 1289
ApoI	RAATTY	1 cut(s)	628
AspLEI	GCGC	2 cut(s)	1196, 1198
AspS9I	GGNCC	2 cut(s)	71, 1076
AsuHPI	GGTGA	6 cut(s)	28, 147, 326, 428, 990, 1052
AvaI	CYCGRG	1 cut(s)	115
AvaII	GGWCC	2 cut(s)	71, 1076
BamHI	GGATCC	1 cut(s)	1017
BanI	GGYRCC	3 cut(s)	198, 652, 937
BbvI	GCAGC	6 cut(s)	204, 475, 605, 748, 1114, 1276
BccI	CCATC	4 cut(s)	95, 302, 814, 1291
BceAI	ACGGC	3 cut(s)	247, 377, 666
BciT130I	CCWGG	2 cut(s)	350, 831
BcoDI	GTCTC	3 cut(s)	419, 720, 1150
BfuAI	ACCTGC	1 cut(s)	1264
BglI	GCCNNNNNGGC	1 cut(s)	398
Bme1390I	CCNGG	2 cut(s)	350, 831
Bme18I	GGWCC	2 cut(s)	71, 1076
BmeT110I	CYCGRG	1 cut(s)	115
BmgT120I	GGNCC	2 cut(s)	71, 1076
BmiI	GGNNCC	7 cut(s)	73, 200, 654, 939, 1019, 1077, 1078
BmrFI	CCNGG	2 cut(s)	350, 831
BmsI	GCATC	1 cut(s)	475
BpmI	CTGGAG	3 cut(s)	4, 498, 1088
BpuEI	CTTGAG	2 cut(s)	542, 585
BsaBI	GATNNNNATC	1 cut(s)	81
BsaJI	CCNNGG	4 cut(s)	94, 202, 349, 843
BsaWI	WCCGGW	1 cut(s)	331
BsaXI	ACNNNNNCTCC	2 cut(s)	115, 145
Bsc4I	CCNNNNNNNGG	2 cut(s)	338, 1273
Bse118I	RCCGGY	1 cut(s)	1109
Bse1I	ACTGG	1 cut(s)	1071
Bse8I	GATNNNNATC	1 cut(s)	81
BseAI	TCCGGA	1 cut(s)	331
BseBI	CCWGG	2 cut(s)	350, 831
BseDI	CCNNGG	4 cut(s)	94, 202, 349, 843
BseGI	GGATG	1 cut(s)	191
BseJI	GATNNNNATC	1 cut(s)	81
BseLI	CCNNNNNNNGG	2 cut(s)	338, 1273
BseMII	CTCAG	2 cut(s)	414, 695
BseNI	ACTGG	1 cut(s)	1071
BsePI	GCGCGC	1 cut(s)	1194
BseRI	GAGGAG	5 cut(s)	23, 398, 507, 740, 1168
BseX3I	CGGCCG	1 cut(s)	205
BseXI	GCAGC	6 cut(s)	204, 475, 605, 748, 1114, 1276
BsgI	GTGCAG	3 cut(s)	95, 414, 969
Bsh1236I	CGCG	3 cut(s)	69, 204, 1196
Bsh1285I	CGRYCG	2 cut(s)	67, 208
BshFI	GGCC	3 cut(s)	102, 207, 389
BshNI	GGYRCC	3 cut(s)	198, 652, 937
BsiEI	CGRYCG	2 cut(s)	67, 208
BsiHKCI	CYCGRG	1 cut(s)	115
BsiSI	CCGG	2 cut(s)	332, 1110
BslFI	GGGAC	3 cut(s)	57, 113, 1062
BslI	CCNNNNNNNGG	2 cut(s)	338, 1273
BsmAI	GTCTC	3 cut(s)	419, 720, 1150
BsmFI	GGGAC	3 cut(s)	57, 113, 1062
BsmI	GAATGC	1 cut(s)	1057
BsnI	GGCC	3 cut(s)	102, 207, 389
BsoBI	CYCGRG	1 cut(s)	115
Bsp13I	TCCGGA	1 cut(s)	331
Bsp143I	GATC	5 cut(s)	64, 82, 440, 876, 1017
BspANI	GGCC	3 cut(s)	102, 207, 389
BspCNI	CTCAG	2 cut(s)	415, 696
BspEI	TCCGGA	1 cut(s)	331
BspFNI	CGCG	3 cut(s)	69, 204, 1196
BspHI	TCATGA	1 cut(s)	1317
BspLI	GGNNCC	7 cut(s)	73, 200, 654, 939, 1019, 1077, 1078
BspMI	ACCTGC	1 cut(s)	1264
BspPI	GGATC	5 cut(s)	77, 448, 871, 1012, 1025
BspT107I	GGYRCC	3 cut(s)	198, 652, 937
BsrFI	RCCGGY	1 cut(s)	1109
BsrI	ACTGG	1 cut(s)	1071
BssAI	RCCGGY	1 cut(s)	1109
BssECI	CCNNGG	4 cut(s)	94, 202, 349, 843
BssHII	GCGCGC	1 cut(s)	1194
BssMI	GATC	5 cut(s)	64, 82, 440, 876, 1017
BssNAI	GTATAC	1 cut(s)	1309
BssT1I	CCWWGG	1 cut(s)	843
Bst1107I	GTATAC	1 cut(s)	1309
Bst2UI	CCWGG	2 cut(s)	350, 831
Bst4CI	ACNGT	3 cut(s)	127, 714, 962
Bst6I	CTCTTC	1 cut(s)	315
BstC8I	GCNNGC	4 cut(s)	260, 1196, 1200, 1239
BstDEI	CTNAG	3 cut(s)	423, 704, 1185
BstDSI	CCRYGG	1 cut(s)	202
BstENI	CCTNNNNNAGG	1 cut(s)	1271
BstF5I	GGATG	1 cut(s)	191
BstFNI	CGCG	3 cut(s)	69, 204, 1196
BstHHI	GCGC	2 cut(s)	1196, 1198
BstKTI	GATC	5 cut(s)	67, 85, 443, 879, 1020
BstMAI	GTCTC	3 cut(s)	419, 720, 1150
BstMBI	GATC	5 cut(s)	64, 82, 440, 876, 1017
BstMCI	CGRYCG	2 cut(s)	67, 208
BstMWI	GCNNNNNNNGC	8 cut(s)	207, 252, 255, 398, 428, 488, 761, 1247
BstNI	CCWGG	2 cut(s)	350, 831
BstSCI	CCNGG	2 cut(s)	348, 829
BstUI	CGCG	3 cut(s)	69, 204, 1196
BstV1I	GCAGC	6 cut(s)	204, 475, 605, 748, 1114, 1276
BstX2I	RGATCY	1 cut(s)	1017
BstYI	RGATCY	1 cut(s)	1017
BstZ17I	GTATAC	1 cut(s)	1309
BstZI	CGGCCG	1 cut(s)	205
BsuRI	GGCC	3 cut(s)	102, 207, 389
BtgI	CCRYGG	1 cut(s)	202
BtsCI	GGATG	1 cut(s)	191
BveI	ACCTGC	1 cut(s)	1264
Cac8I	GCNNGC	4 cut(s)	260, 1196, 1200, 1239
CciI	TCATGA	1 cut(s)	1317
CciNI	GCGGCCGC	1 cut(s)	205
CfoI	GCGC	2 cut(s)	1196, 1198
Cfr10I	RCCGGY	1 cut(s)	1109
Cfr13I	GGNCC	2 cut(s)	71, 1076
Cfr42I	CCGCGG	1 cut(s)	205
CseI	GACGC	1 cut(s)	223
CsiI	ACCWGGT	1 cut(s)	829
CviAII	CATG	3 cut(s)	874, 934, 1318
DdeI	CTNAG	3 cut(s)	423, 704, 1185
DpnI	GATC	5 cut(s)	66, 84, 442, 878, 1019
DpnII	GATC	5 cut(s)	64, 82, 440, 876, 1017
DraIII	CACNNNGTG	1 cut(s)	833
EaeI	YGGCCR	1 cut(s)	205
EagI	CGGCCG	1 cut(s)	205
Eam1104I	CTCTTC	1 cut(s)	315
EarI	CTCTTC	1 cut(s)	315
EciI	GGCGGA	1 cut(s)	857
EclXI	CGGCCG	1 cut(s)	205
Eco130I	CCWWGG	1 cut(s)	843
Eco147I	AGGCCT	1 cut(s)	102
Eco47I	GGWCC	2 cut(s)	71, 1076
Eco52I	CGGCCG	1 cut(s)	205
Eco57I	CTGAAG	5 cut(s)	629, 906, 927, 1023, 1143
Eco88I	CYCGRG	1 cut(s)	115
EcoNI	CCTNNNNNAGG	1 cut(s)	1271
EcoO109I	RGGNCCY	1 cut(s)	1076
EcoRII	CCWGG	2 cut(s)	348, 829
EcoT14I	CCWWGG	1 cut(s)	843
ErhI	CCWWGG	1 cut(s)	843
FaeI	CATG	3 cut(s)	877, 937, 1321
FaqI	GGGAC	3 cut(s)	57, 113, 1062
FatI	CATG	3 cut(s)	873, 933, 1317
FauNDI	CATATG	1 cut(s)	1026
FblI	GTMKAC	2 cut(s)	1308, 1343
FokI	GGATG	1 cut(s)	198
GlaI	GCGC	2 cut(s)	1195, 1197
GsuI	CTGGAG	3 cut(s)	4, 498, 1088
HaeIII	GGCC	3 cut(s)	102, 207, 389
HapII	CCGG	2 cut(s)	332, 1110
HgaI	GACGC	1 cut(s)	223
HhaI	GCGC	2 cut(s)	1196, 1198
Hin1II	CATG	3 cut(s)	877, 937, 1321
Hin6I	GCGC	2 cut(s)	1194, 1196
HinP1I	GCGC	2 cut(s)	1194, 1196
HindIII	AAGCTT	1 cut(s)	370
HinfI	GANTC	4 cut(s)	77, 335, 728, 1340
HpaII	CCGG	2 cut(s)	332, 1110
HphI	GGTGA	6 cut(s)	28, 147, 326, 428, 990, 1052
Hpy166II	GTNNAC	4 cut(s)	732, 914, 1309, 1344
Hpy188I	TCNGA	7 cut(s)	64, 82, 98, 382, 705, 720, 1123
Hpy8I	GTNNAC	4 cut(s)	732, 914, 1309, 1344
Hpy99I	CGWCG	1 cut(s)	398
HpyCH4III	ACNGT	3 cut(s)	127, 714, 962
HpyCH4IV	ACGT	1 cut(s)	506
HpyCH4V	TGCA	8 cut(s)	112, 431, 593, 950, 1171, 1202, 1250, 1273
HpyF10VI	GCNNNNNNNGC	8 cut(s)	207, 252, 255, 398, 428, 488, 761, 1247
HpyF3I	CTNAG	3 cut(s)	423, 704, 1185
HpySE526I	ACGT	1 cut(s)	506
Hsp92II	CATG	3 cut(s)	877, 937, 1321
HspAI	GCGC	2 cut(s)	1194, 1196
KflI	GGGWCCC	1 cut(s)	1076
Kpn2I	TCCGGA	1 cut(s)	331
KspI	CCGCGG	1 cut(s)	205
Kzo9I	GATC	5 cut(s)	64, 82, 440, 876, 1017
LmnI	GCTCC	3 cut(s)	9, 479, 520
Lsp1109I	GCAGC	6 cut(s)	204, 475, 605, 748, 1114, 1276
LweI	GCATC	1 cut(s)	475
MabI	ACCWGGT	1 cut(s)	829
MaeII	ACGT	1 cut(s)	506
MaeIII	GTNAC	1 cut(s)	119
MalI	GATC	5 cut(s)	66, 84, 442, 878, 1019
MboI	GATC	5 cut(s)	64, 82, 440, 876, 1017
MboII	GAAGA	7 cut(s)	82, 252, 332, 574, 635, 983, 1333
MflI	RGATCY	1 cut(s)	1017
MluCI	AATT	6 cut(s)	628, 670, 736, 771, 1046, 1215
MlyI	GAGTC	2 cut(s)	737, 1349
MmeI	TCCRAC	1 cut(s)	1045
MroI	TCCGGA	1 cut(s)	331
MseI	TTAA	5 cut(s)	194, 660, 1190, 1212, 1256
MspA1I	CMGCKG	1 cut(s)	204
MspI	CCGG	2 cut(s)	332, 1110
MspR9I	CCNGG	2 cut(s)	350, 831
Mva1269I	GAATGC	1 cut(s)	1057
MvaI	CCWGG	2 cut(s)	350, 831
MvnI	CGCG	3 cut(s)	69, 204, 1196
MwoI	GCNNNNNNNGC	8 cut(s)	207, 252, 255, 398, 428, 488, 761, 1247
NdeI	CATATG	1 cut(s)	1026
NdeII	GATC	5 cut(s)	64, 82, 440, 876, 1017
NlaIII	CATG	3 cut(s)	877, 937, 1321
NlaIV	GGNNCC	7 cut(s)	73, 200, 654, 939, 1019, 1077, 1078
NotI	GCGGCCGC	1 cut(s)	205
PagI	TCATGA	1 cut(s)	1317
PauI	GCGCGC	1 cut(s)	1194
PceI	AGGCCT	1 cut(s)	102
PctI	GAATGC	1 cut(s)	1057
PfeI	GAWTC	2 cut(s)	77, 335
Ple19I	CGATCG	1 cut(s)	67
PleI	GAGTC	2 cut(s)	736, 1348
PpsI	GAGTC	2 cut(s)	736, 1348
PpuMI	RGGWCCY	1 cut(s)	1076
Psp5II	RGGWCCY	1 cut(s)	1076
Psp6I	CCWGG	2 cut(s)	348, 829
PspGI	CCWGG	2 cut(s)	348, 829
PspN4I	GGNNCC	7 cut(s)	73, 200, 654, 939, 1019, 1077, 1078
PspPI	GGNCC	2 cut(s)	71, 1076
PspPPI	RGGWCCY	1 cut(s)	1076
PsuI	RGATCY	1 cut(s)	1017
PteI	GCGCGC	1 cut(s)	1194
PvuI	CGATCG	1 cut(s)	67
SacII	CCGCGG	1 cut(s)	205
SaqAI	TTAA	5 cut(s)	194, 660, 1190, 1212, 1256
Sau3AI	GATC	5 cut(s)	64, 82, 440, 876, 1017
Sau96I	GGNCC	2 cut(s)	71, 1076
SchI	GAGTC	2 cut(s)	737, 1349
ScrFI	CCNGG	2 cut(s)	350, 831
SexAI	ACCWGGT	1 cut(s)	829
SfaNI	GCATC	1 cut(s)	475
Sfr303I	CCGCGG	1 cut(s)	205
SgrAI	CRCCGGYG	1 cut(s)	1109
SgrBI	CCGCGG	1 cut(s)	205
SinI	GGWCC	2 cut(s)	71, 1076
SmlI	CTYRAG	2 cut(s)	521, 600
SmoI	CTYRAG	2 cut(s)	521, 600
Sse9I	AATT	6 cut(s)	628, 670, 736, 771, 1046, 1215
SseBI	AGGCCT	1 cut(s)	102
SspI	AATATT	1 cut(s)	640
StuI	AGGCCT	1 cut(s)	102
StyD4I	CCNGG	2 cut(s)	348, 829
StyI	CCWWGG	1 cut(s)	843
TaaI	ACNGT	3 cut(s)	127, 714, 962
TaiI	ACGT	1 cut(s)	509
TaqI	TCGA	6 cut(s)	287, 408, 923, 1177, 1221, 1338
TasI	AATT	6 cut(s)	628, 670, 736, 771, 1046, 1215
TauI	GCSGC	7 cut(s)	204, 207, 210, 249, 258, 392, 767
TfiI	GAWTC	2 cut(s)	77, 335
Tru1I	TTAA	5 cut(s)	194, 660, 1190, 1212, 1256
Tru9I	TTAA	5 cut(s)	194, 660, 1190, 1212, 1256
TseI	GCWGC	6 cut(s)	217, 488, 593, 761, 1127, 1289
TspDTI	ATGAA	7 cut(s)	34, 366, 467, 983, 1043, 1306, 1334
TspGWI	ACGGA	2 cut(s)	155, 448
VpaK11BI	GGWCC	2 cut(s)	71, 1076
XagI	CCTNNNNNAGG	1 cut(s)	1271
XapI	RAATTY	1 cut(s)	628
XmiI	GTMKAC	2 cut(s)	1308, 1343

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.