Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002015

Physical Location & Seq

Genomic Coordinates Reverse (-)

33278270 .. 33282901

Gene Structure 4632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1320300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1413 bp

ATGAAGCTGGATGTGAACATGTTGAGATACCTCTCCAAAGATGATTTTAGGGTTCTCACTGCTGTGGAAATGGGAATGAGAAATCATGAAATTGTACCCTGTGAACTGGTGGATCGGATAGCGTCGCTCAAGCATGGTGGTACTTATAAGGTTCTCAAGAATCTCCTCAGGCATAAGCTCTTGCATCACGACTCTTCTAAATATGACGGGTTTCGACTCACCTACCTTGGTTATGATTTTCTTGCAATTAAGACTCTGGTTAATCGTGGAGTCTTTGTGTCTGTTGGCCGTCAAATTGGCGTAGGAAAAGAGTCTGATATCTTTGAGGTCGCCACAGAAGATGGTACGGTTATGGCAATGAAGTTGCACCGGCTTGGTAGAGTTTCTTTTAGGGCTGTAAAAACTAAGCGTGATTACTTGAAACATCGTAGCAGTTATAGTTGGCTCTACTTGTCCCGCCTTGCTGCACTCAAAGAATTTGCTTTTATGAAGGCTTTAGAAGAGCATGGCTTTCCTGTTCCAAGTGCTGTGGATTGTAACAGGCATTGTGTGATTATGTCACTTGTACAAGGATACCCACTTGTGCAGGTGAAGCAACTGCAGAACCCAGAGGTGGTTTTTGAAACAATCATCGGACTTGTCGTTCGCCTTGCTGAACATGGTTTAATTCACTGTGACTTCAATGAATTTAACATTATGATTGATGATGACGAGAAGGTCACCATGATTGATTTTCCACAGATGGTGTCTGTATCACATCATAATGCACAGATGTACTTTGACCGTGATGTTGAATGTGTCTTTAAGTTTTTTAGAAAGAGGTTCAACATGAATTTTCAAGAGTGCAGAGATGATATTGATAGAACAGAGGTAGACACAGATGAAAGTGGGAGGCTTTGTTTTTCTTCAATAGCCAAGGATGCTGTTTTTCTTGACAAGGAACTTGCTGCCAGTGGGTTTACTAGGAAGGATCAGGAAGAGATTGAGAAGTTCATTGAAGGAAGTTTGGATAAGGATGCTAGTTCTGCTGATGAAGGTACTGAAGATGACACAGACAACTCTGCGTTAAATGAATCAAACATAGAGGGTGTTGATTCATTGAATTTGTTAGATCAGGACCAGACTTCAAATTTAAAGCAGGAAGAGGAAGGAGCAGTGGACAATCTAAGAAACTGTGAAGCAGGCCCAAGCAGTAAACCCGAATGTGAAGATGTGAGTGACAAGGAGGGAGATAATGACACCGAAAATGGAAATGATGCTGAGCTGGTAAAGCGCTTGAGCAAGCAGAGACGACGTGCCATGACAGCAGCCCGCGGAGGACGGAGGACTCTTTCCTCCAGAAATACCTACAAAGATAAGGGCGGTAGATCCTCCAACAAATCCAAAATCCAGAATCAATTAAGCAGCTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

RIO1 Rio2_N

Protein Analysis

471

Amino Acids

53.48

Weight (kDa)

5.65

Isoelectric Point (pI)

42.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rio2_N	PF09202	8 - 89	1e-33	Rio2, N-terminal
RIO1	PF01163	106 - 280	4.3e-55	RIO domain
ABC1	PF03109	170 - 260	2.1e-07	ABC1 atypical kinase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002015)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51270 AT3G51270 AT3G51270 AT3G51270
fragaria_vesca	FvH4_4g10760 FvH4_4g10760 FvH4_4g10760 FvH4_4g10760 FvH4_7g06440 FvH4_7g06440
malus_domestica	MD01G1035500.v1.1 MD02G1001000.v1.1 MD15G1320300.v1.1
prunus_persica	Prupe.5G084300_v2.0.a1 Prupe.5G084300_v2.0.a1 Prupe.5G084300_v2.0.a1 Prupe.5G084300_v2.0.a1
pyrus_communis	pycom01g06260 pycom15g28140
rosa_chinensis	RchiOBHm_Chr1g0334711 RchiOBHm_Chr7g0210541
rosa_laevigata	RLG00000026279 RLG00000029431
rosa_multiflora	Rmu_sc0002295.1_g000002 Rmu_sc0006031.1_g000024
rosa_roxburghii	Rroxscaffold_3G00236050 Rroxscaffold_4G00279110 Rroxscaffold_4G00316660
rosa_rugosa	Rorug01G0115100 Rorug01G0115200 Rorug07G0122000 Rorug07G0122100 Rorug07G0122100 Rorug07G0122200 Rorug07G0122200
rosa_samantha	Rh1AG140300 Rh1BG107400 Rh1CG132200 Rh1DG144300 Rh7AG254300 Rh7BG249600 Rh7CG271200 Rh7DG262000
rosa_wichuraiana	Rw1G011570 Rw7G021730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	147
AarI	CACCTGC	1 cut(s)	577
AasI	GACNNNNNNGTC	1 cut(s)	716
Acc36I	ACCTGC	1 cut(s)	577
AccI	GTMKAC	1 cut(s)	873
AccII	CGCG	1 cut(s)	1314
AciI	CCGC	4 cut(s)	457, 1312, 1314, 1362
AclWI	GGATC	3 cut(s)	120, 978, 1362
AcoI	YGGCCR	1 cut(s)	286
AcsI	RAATTY	5 cut(s)	476, 686, 832, 1102, 1129
AcuI	CTGAAG	1 cut(s)	1062
AfaI	GTAC	6 cut(s)	96, 142, 346, 567, 776, 1039
AfeI	AGCGCT	1 cut(s)	1274
AfiI	CCNNNNNNNGG	1 cut(s)	613
AflIII	ACRYGT	1 cut(s)	18
AjiI	CACGTC	1 cut(s)	1295
AleI	CACNNNNGTG	1 cut(s)	62
AloI	GAACNNNNNNTCC	2 cut(s)	627, 659
AluBI	AGCT	4 cut(s)	7, 178, 1264, 1407
AluI	AGCT	4 cut(s)	7, 178, 1264, 1407
Alw26I	GTCTC	1 cut(s)	1282
AlwI	GGATC	3 cut(s)	120, 978, 1362
Aor51HI	AGCGCT	1 cut(s)	1274
AoxI	GGCC	2 cut(s)	286, 1183
ApeKI	GCWGC	4 cut(s)	464, 947, 1307, 1404
ApoI	RAATTY	5 cut(s)	476, 686, 832, 1102, 1129
ArsI	GACNNNNNNTTYG	2 cut(s)	627, 659
AspLEI	GCGC	1 cut(s)	1275
AspS9I	GGNCC	2 cut(s)	1117, 1184
AsuHPI	GGTGA	3 cut(s)	211, 601, 712
AvaII	GGWCC	1 cut(s)	1117
AxyI	CCTNAGG	1 cut(s)	167
BbvI	GCAGC	3 cut(s)	451, 934, 1319
BccI	CCATC	2 cut(s)	335, 736
BceAI	ACGGC	1 cut(s)	273
BciVI	GTATCC	1 cut(s)	566
BcoDI	GTCTC	1 cut(s)	1282
BfaI	CTAG	2 cut(s)	963, 1020
BfmI	CTRYAG	1 cut(s)	599
BfoI	RGCGCY	1 cut(s)	1276
BfuAI	ACCTGC	1 cut(s)	577
BfuI	GTATCC	1 cut(s)	566
BisI	GCNGC	4 cut(s)	465, 948, 1308, 1405
BlpI	GCTNAGC	1 cut(s)	1260
BlsI	GCNGC	4 cut(s)	466, 949, 1309, 1406
Bme18I	GGWCC	1 cut(s)	1117
BmgBI	CACGTC	1 cut(s)	1295
BmgT120I	GGNCC	2 cut(s)	1117, 1184
BmsI	GCATC	4 cut(s)	193, 910, 1006, 1246
BpmI	CTGGAG	1 cut(s)	1321
Bpu1102I	GCTNAGC	1 cut(s)	1260
BpuEI	CTTGAG	3 cut(s)	113, 140, 1297
BsaJI	CCNNGG	3 cut(s)	226, 915, 1312
BsaXI	ACNNNNNCTCC	4 cut(s)	261, 291, 883, 913
Bsc4I	CCNNNNNNNGG	1 cut(s)	613
Bse118I	RCCGGY	1 cut(s)	369
Bse1I	ACTGG	2 cut(s)	111, 951
Bse21I	CCTNAGG	1 cut(s)	167
Bse3DI	GCAATG	1 cut(s)	363
BseDI	CCNNGG	3 cut(s)	226, 915, 1312
BseGI	GGATG	3 cut(s)	16, 925, 1021
BseLI	CCNNNNNNNGG	1 cut(s)	613
BseMI	GCAATG	1 cut(s)	363
BseMII	CTCAG	2 cut(s)	181, 1251
BseNI	ACTGG	2 cut(s)	111, 951
BseRI	GAGGAG	1 cut(s)	155
BseXI	GCAGC	3 cut(s)	451, 934, 1319
BsgI	GTGCAG	3 cut(s)	450, 605, 865
Bsh1236I	CGCG	1 cut(s)	1314
BshFI	GGCC	2 cut(s)	288, 1185
BsiSI	CCGG	1 cut(s)	370
BslFI	GGGAC	1 cut(s)	439
BslI	CCNNNNNNNGG	1 cut(s)	613
BsmAI	GTCTC	1 cut(s)	1282
BsmBI	CGTCTC	1 cut(s)	1282
BsmFI	GGGAC	1 cut(s)	439
BsnI	GGCC	2 cut(s)	288, 1185
Bsp1407I	TGTACA	1 cut(s)	565
Bsp143I	GATC	4 cut(s)	112, 970, 1111, 1367
Bsp1720I	GCTNAGC	1 cut(s)	1260
BspACI	CCGC	4 cut(s)	457, 1312, 1314, 1362
BspANI	GGCC	2 cut(s)	288, 1185
BspCNI	CTCAG	2 cut(s)	180, 1252
BspFNI	CGCG	1 cut(s)	1314
BspHI	TCATGA	1 cut(s)	85
BspMAI	CTGCAG	1 cut(s)	603
BspMI	ACCTGC	1 cut(s)	577
BspPI	GGATC	3 cut(s)	120, 978, 1362
BspQI	GCTCTTC	1 cut(s)	495
BsrDI	GCAATG	1 cut(s)	363
BsrFI	RCCGGY	1 cut(s)	369
BsrGI	TGTACA	1 cut(s)	565
BsrI	ACTGG	2 cut(s)	111, 951
BssAI	RCCGGY	1 cut(s)	369
BssECI	CCNNGG	3 cut(s)	226, 915, 1312
BssMI	GATC	4 cut(s)	112, 970, 1111, 1367
BssT1I	CCWWGG	2 cut(s)	226, 915
Bst4CI	ACNGT	4 cut(s)	349, 674, 785, 1175
Bst6I	CTCTTC	4 cut(s)	199, 495, 972, 1137
BstAUI	TGTACA	1 cut(s)	565
BstC8I	GCNNGC	3 cut(s)	1183, 1283, 1312
BstDEI	CTNAG	4 cut(s)	167, 405, 1166, 1260
BstDSI	CCRYGG	1 cut(s)	1312
BstEII	GGTNACC	1 cut(s)	718
BstF5I	GGATG	3 cut(s)	16, 925, 1021
BstFNI	CGCG	1 cut(s)	1314
BstH2I	RGCGCY	1 cut(s)	1276
BstHHI	GCGC	1 cut(s)	1275
BstKTI	GATC	4 cut(s)	115, 973, 1114, 1370
BstMAI	GTCTC	1 cut(s)	1282
BstMBI	GATC	4 cut(s)	112, 970, 1111, 1367
BstMWI	GCNNNNNNNGC	5 cut(s)	592, 920, 1025, 1270, 1304
BstNSI	RCATGY	1 cut(s)	22
BstPI	GGTNACC	1 cut(s)	718
BstSFI	CTRYAG	1 cut(s)	599
BstUI	CGCG	1 cut(s)	1314
BstV1I	GCAGC	3 cut(s)	451, 934, 1319
BstX2I	RGATCY	1 cut(s)	1367
BstYI	RGATCY	1 cut(s)	1367
Bsu36I	CCTNAGG	1 cut(s)	167
BsuI	GTATCC	1 cut(s)	566
BsuRI	GGCC	2 cut(s)	288, 1185
BtgI	CCRYGG	1 cut(s)	1312
BtrI	CACGTC	1 cut(s)	1295
BtsCI	GGATG	3 cut(s)	16, 925, 1021
BtsI	GCAGTG	2 cut(s)	57, 1161
BtsIMutI	CAGTG	4 cut(s)	57, 670, 958, 1161
BveI	ACCTGC	1 cut(s)	577
Cac8I	GCNNGC	3 cut(s)	1183, 1283, 1312
CciI	TCATGA	1 cut(s)	85
CfoI	GCGC	1 cut(s)	1275
Cfr10I	RCCGGY	1 cut(s)	369
Cfr13I	GGNCC	2 cut(s)	1117, 1184
Cfr42I	CCGCGG	1 cut(s)	1315
CseI	GACGC	1 cut(s)	111
Csp6I	GTAC	6 cut(s)	95, 141, 345, 566, 775, 1038
CspCI	CAANNNNNGTGG	4 cut(s)	118, 153, 510, 545
CviAII	CATG	8 cut(s)	19, 86, 134, 506, 659, 724, 829, 1300
CviQI	GTAC	6 cut(s)	95, 141, 345, 566, 775, 1038
DdeI	CTNAG	4 cut(s)	167, 405, 1166, 1260
DpnI	GATC	4 cut(s)	114, 972, 1113, 1369
DpnII	GATC	4 cut(s)	112, 970, 1111, 1367
DraI	TTTAAA	1 cut(s)	1134
DrdI	GACNNNNNNGTC	1 cut(s)	716
DseDI	GACNNNNNNGTC	1 cut(s)	716
EaeI	YGGCCR	1 cut(s)	286
Eam1104I	CTCTTC	4 cut(s)	199, 495, 972, 1137
EarI	CTCTTC	4 cut(s)	199, 495, 972, 1137
Eco130I	CCWWGG	2 cut(s)	226, 915
Eco32I	GATATC	1 cut(s)	319
Eco47I	GGWCC	1 cut(s)	1117
Eco47III	AGCGCT	1 cut(s)	1274
Eco57I	CTGAAG	1 cut(s)	1062
Eco81I	CCTNAGG	1 cut(s)	167
Eco91I	GGTNACC	1 cut(s)	718
EcoO65I	GGTNACC	1 cut(s)	718
EcoRV	GATATC	1 cut(s)	319
EcoT14I	CCWWGG	2 cut(s)	226, 915
ErhI	CCWWGG	2 cut(s)	226, 915
Esp3I	CGTCTC	1 cut(s)	1282
FaeI	CATG	8 cut(s)	22, 89, 137, 509, 662, 727, 832, 1303
FaqI	GGGAC	1 cut(s)	439
FatI	CATG	8 cut(s)	18, 85, 133, 505, 658, 723, 828, 1299
FauI	CCCGC	2 cut(s)	464, 1319
FblI	GTMKAC	1 cut(s)	873
Fnu4HI	GCNGC	4 cut(s)	465, 948, 1308, 1405
FokI	GGATG	3 cut(s)	23, 932, 1028
Fsp4HI	GCNGC	4 cut(s)	465, 948, 1308, 1405
FspBI	CTAG	2 cut(s)	963, 1020
GlaI	GCGC	1 cut(s)	1274
GluI	GCNGC	4 cut(s)	465, 948, 1308, 1405
GsuI	CTGGAG	1 cut(s)	1321
HaeII	RGCGCY	1 cut(s)	1276
HaeIII	GGCC	2 cut(s)	288, 1185
HapII	CCGG	1 cut(s)	370
HgaI	GACGC	1 cut(s)	111
HhaI	GCGC	1 cut(s)	1275
Hin1II	CATG	8 cut(s)	22, 89, 137, 509, 662, 727, 832, 1303
Hin6I	GCGC	1 cut(s)	1273
HinP1I	GCGC	1 cut(s)	1273
HpaII	CCGG	1 cut(s)	370
HphI	GGTGA	3 cut(s)	211, 601, 712
Hpy166II	GTNNAC	6 cut(s)	16, 104, 874, 960, 1159, 1196
Hpy188I	TCNGA	3 cut(s)	117, 316, 635
Hpy188III	TCNNGA	9 cut(s)	86, 157, 188, 839, 932, 974, 1115, 1338, 1390
Hpy8I	GTNNAC	6 cut(s)	16, 104, 874, 960, 1159, 1196
Hpy99I	CGWCG	2 cut(s)	127, 1296
HpyAV	CCTTC	6 cut(s)	484, 709, 961, 992, 1028, 1142
HpyCH4III	ACNGT	4 cut(s)	349, 674, 785, 1175
HpyCH4IV	ACGT	1 cut(s)	1294
HpyCH4V	TGCA	8 cut(s)	184, 245, 367, 467, 586, 601, 767, 846
HpyF10VI	GCNNNNNNNGC	5 cut(s)	592, 920, 1025, 1270, 1304
HpyF3I	CTNAG	4 cut(s)	167, 405, 1166, 1260
HpySE526I	ACGT	1 cut(s)	1294
Hsp92II	CATG	8 cut(s)	22, 89, 137, 509, 662, 727, 832, 1303
HspAI	GCGC	1 cut(s)	1273
KspI	CCGCGG	1 cut(s)	1315
Kzo9I	GATC	4 cut(s)	112, 970, 1111, 1367
LguI	GCTCTTC	1 cut(s)	495
LmnI	GCTCC	1 cut(s)	1151
Lsp1109I	GCAGC	3 cut(s)	451, 934, 1319
LweI	GCATC	4 cut(s)	193, 910, 1006, 1246
MaeI	CTAG	2 cut(s)	963, 1020
MaeII	ACGT	1 cut(s)	1294
MaeIII	GTNAC	5 cut(s)	536, 558, 674, 718, 1217
MalI	GATC	4 cut(s)	114, 972, 1113, 1369
MboI	GATC	4 cut(s)	112, 970, 1111, 1367
MboII	GAAGA	8 cut(s)	186, 350, 512, 897, 989, 1055, 1154, 1220
MflI	RGATCY	1 cut(s)	1367
MlyI	GAGTC	6 cut(s)	185, 210, 247, 279, 320, 1321
MmeI	TCCRAC	1 cut(s)	1398
MseI	TTAA	8 cut(s)	249, 261, 665, 690, 804, 1067, 1133, 1400
MslI	CAYNNNNRTG	2 cut(s)	62, 762
MspA1I	CMGCKG	2 cut(s)	1314, 1407
MspI	CCGG	1 cut(s)	370
MvnI	CGCG	1 cut(s)	1314
MwoI	GCNNNNNNNGC	5 cut(s)	592, 920, 1025, 1270, 1304
NdeII	GATC	4 cut(s)	112, 970, 1111, 1367
NlaIII	CATG	8 cut(s)	22, 89, 137, 509, 662, 727, 832, 1303
NmuCI	GTSAC	4 cut(s)	558, 674, 718, 1217
NspI	RCATGY	1 cut(s)	22
OliI	CACNNNNGTG	1 cut(s)	62
PagI	TCATGA	1 cut(s)	85
PaqCI	CACCTGC	1 cut(s)	577
PciI	ACATGT	1 cut(s)	18
PciSI	GCTCTTC	1 cut(s)	495
PcsI	WCGNNNNNNNCGW	1 cut(s)	639
PfeI	GAWTC	4 cut(s)	160, 1073, 1094, 1393
PkrI	GCNGC	4 cut(s)	466, 949, 1309, 1406
PleI	GAGTC	6 cut(s)	185, 210, 247, 278, 319, 1321
PpsI	GAGTC	6 cut(s)	185, 210, 247, 278, 319, 1321
PscI	ACATGT	1 cut(s)	18
PsiI	TTATAA	1 cut(s)	147
PspEI	GGTNACC	1 cut(s)	718
PspPI	GGNCC	2 cut(s)	1117, 1184
PstI	CTGCAG	1 cut(s)	603
PsuI	RGATCY	1 cut(s)	1367
PvuII	CAGCTG	1 cut(s)	1407
RsaI	GTAC	6 cut(s)	96, 142, 346, 567, 776, 1039
RsaNI	GTAC	6 cut(s)	95, 141, 345, 566, 775, 1038
RseI	CAYNNNNRTG	2 cut(s)	62, 762
SacII	CCGCGG	1 cut(s)	1315
SapI	GCTCTTC	1 cut(s)	495
SaqAI	TTAA	8 cut(s)	249, 261, 665, 690, 804, 1067, 1133, 1400
SatI	GCNGC	4 cut(s)	465, 948, 1308, 1405
Sau3AI	GATC	4 cut(s)	112, 970, 1111, 1367
Sau96I	GGNCC	2 cut(s)	1117, 1184
SchI	GAGTC	6 cut(s)	185, 210, 247, 279, 320, 1321
SfaNI	GCATC	4 cut(s)	193, 910, 1006, 1246
SfcI	CTRYAG	1 cut(s)	599
Sfr303I	CCGCGG	1 cut(s)	1315
SgrBI	CCGCGG	1 cut(s)	1315
SinI	GGWCC	1 cut(s)	1117
SmiMI	CAYNNNNRTG	2 cut(s)	62, 762
SmlI	CTYRAG	3 cut(s)	128, 155, 1276
SmoI	CTYRAG	3 cut(s)	128, 155, 1276
SsiI	CCGC	4 cut(s)	457, 1312, 1314, 1362
SspMI	CTAG	2 cut(s)	963, 1020
StyI	CCWWGG	2 cut(s)	226, 915
TaaI	ACNGT	4 cut(s)	349, 674, 785, 1175
TaiI	ACGT	1 cut(s)	1297
TaqI	TCGA	1 cut(s)	214
TatI	WGTACW	2 cut(s)	565, 774
TfiI	GAWTC	4 cut(s)	160, 1073, 1094, 1393
Tru1I	TTAA	8 cut(s)	249, 261, 665, 690, 804, 1067, 1133, 1400
Tru9I	TTAA	8 cut(s)	249, 261, 665, 690, 804, 1067, 1133, 1400
TscAI	CASTG	4 cut(s)	64, 677, 958, 1161
TseFI	GTSAC	4 cut(s)	558, 674, 718, 1217
TseI	GCWGC	4 cut(s)	464, 947, 1307, 1404
Tsp45I	GTSAC	4 cut(s)	558, 674, 718, 1217
TspGWI	ACGGA	1 cut(s)	1336
TspRI	CASTG	4 cut(s)	64, 677, 958, 1161
VpaK11BI	GGWCC	1 cut(s)	1117
XapI	RAATTY	5 cut(s)	476, 686, 832, 1102, 1129
XceI	RCATGY	1 cut(s)	22
XmiI	GTMKAC	1 cut(s)	873
XspI	CTAG	2 cut(s)	963, 1020

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.