Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003030

Physical Location & Seq

Genomic Coordinates Forward (+)

42980437 .. 42986103

Gene Structure 5667 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1356500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2448 bp

ATGGACGGGGAGGCTAACCTTCCAGTCCGACCCGCTGCGGCGAGGAATTTGATCCGTTCGGTCAACCTGGATTCCACATCGTCATCGTCTTCGTCGTTCTCGTCTTCCTCCCCGCCGAACTTTCTCCGGAATGTCCAGGCGGCCTTCAAGCGACACCGCCCTCTCGTAATGCAGTCAAATAGCATAAGACCTCGGCGTATGGTGCTCCCCCAGCGAAGCGTATCCAAAAGTGCGGATTCAAGTGTAGCTCCTCTCATTGAATCAAAGCAGTTGAGAGATGCAGCTTCAATGAGTCAGGGTAATACTGCAAAGGAGTCAATTGCGCAGCCAAACAACATGAAGGAAACTCAAGAAGATGCATCTATTACACCATTTTCCACTTCAGGCGTTAGCACCCAAACCTTTGATGAACGATTAAATCCATTTGATGCGCAAATAGATCAAGCCAAAATTGGCGTTAGTGGTAAAGACAATAACTTGATGTCTCCCTTGCACTTGGAGTCCCAACAGATTGATGGTAAAAAAAAGGTCCAATTTTCAACTGGAAGCGTTCCCATTCCCCAGGAAATGGAGTGGATTTCCGGCAGTCAAGTGGATAGATCAGCTGTCACCAATCTTGACTCAAAGCATCAGAATATTCAGAACATGGAATCTGATGTCTGTTTGAAGTCTGATGGTGTAATTTCTTCTTCATTGGCAAAGAGAACAACTGCCATTCAGGACCAGTTGCATCAGTTTAGGAACTTCTTAAGCCAGCCTGCAACTCAATCCTCAGTTGTCGGGCCATCTTGTGCTACAACAACGTCTGTCCATGCTACTTCAGCACCCATGCTTAGTACAACAACTTATTGTTCTCATTTTCATAGCGGTCCTCATGTGGCAGTCGATCCTCAACCCGTAACTCAAGGGAATATGATGCATCCTGCAAAAGCTTCTTTAAAAGTCACCAGTGGAATGTCAACTGCCCAGGTAGCTGTTGCAGACCAAAGTTGTAACTCTGCAATTGATACCCAAATGCAAGTTAAGGAGTATGAACTCTCTAAGGATCCAATTGGCTGCATGTCGAAGGAAAGTAAGATTACAGAACGTCATTCTCTTCTTGATGCCAAGTCAACTGAAGGGAAAGGGCTTACATGTGATGTTACCAATCCACCATCACAGGTTCCTTTGCCCCAAAATTCATGTGCTGATATGAAGTCAGAGCCTTCTAAATCTGAGAAGCAACAAAAGGCTGTAAGTAGTAAAGGTGCATCAGCCCCTCGTAAAAGGAATTATGATCCGGAATTGTTTTTTAAAGTCAACGGGAAGCTCTATCAAAGGCTTGGAAAAATAGGTAGTGGAGGGAGCAGTGAGGTCCACAAAGTCATATCATCAGACTGTACAATCTATGCACTTAAGAAAATCAAGCTGAAAGGGCGTGATTACGCCACTGCTTTTGGCTTTTGTCAAGAAATTGAGTATTTGAATAGGCTGAAGGGAAAAAACCACATCATACAGCTTATTGACTATGAGGTGACAGATAAGGCTTTGCTCCATGAAGTCTTGAGTGGCTCCATGAATAATAAGGATGGCAGAGTCAAGGATGATGGGTATATATACATGGTACTTGAATATGGAGAAATTGATTTGGCTCACATGCTGTCTCAGCAGTGGAAGGAAATGGACGTCTCCAAAAAGACCATAGATGAGAACTGGCTACGATTCTACTGGCAGCAAATTCTTCTAGCGGTTAACACTATACATGAGGAACGTATTGTGCACTCTGACTTGAAGCCAGCCAACTTCCTTCTTGTCAAAGGCTCTCTAAAGCTAATTGATTTTGGGATTGCCAAAGCAATAATGAGTGATACAACGAACATCCAGAGGGATTCTCAGGTGGGTACACTTAGCTACATGTCTCCAGAAGCGTTCATGTGCAACGAGAGTGATGCAGATGGAAACACTATCAAGTGTGGTCGACCATCAGACATCTGGTCGCTCGGCTGCATCCTTTATCAGATGGTATATGGGAGGACACCATTTGCAGACTACAAGACCTTCTGGGCCAAGTTCAAAGTTATAACTGATCCCAACCATGAAATCACATATGAACCAGTCTCAAACCCCTGGCTTATGGACCTCATGAAAAAGTGCCTCGCATGGGACCGCAACGAAAGGTGGAGGATACCTCAGCTACTCCAACACCCTTTCATTGTTCCCCCAGTTCCACAGCAGCCATCTTCGCCTTGCGAGGAAAACTGTAAACTGCTTCAACTTATTTCCCAAACCTGTGCTGACGATCGCGAGGCTTCCAAGCTATGTTGTCAGATGCAGCAACTGCTTAAGGACCCCGTCACGTCAATAGGGTCTCAGTTAGTATCATCACGAGACCTACAATGTAAATTGCTCTCCCAGATGACAAAACTTTGCCTTCAGCTCCAGGAACATTTAGCAAACTCAGAGGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004672 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005813 GO:0005815 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0015630 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0031974 GO:0031981 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044454 GO:0044464 GO:0070013 GO:0071704 GO:0098687 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 map04110 map04111

Pfam Domains

Protein Families

Pkinase

Protein Analysis

816

Amino Acids

90.38

Weight (kDa)

7.95

Isoelectric Point (pI)

48.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	438 - 731	1.4e-54	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	480 - 727	2.4e-20	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003030)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77720
fragaria_vesca	FvH4_2g26330 FvH4_2g26330 FvH4_2g26330 FvH4_2g26330 FvH4_2g26330 FvH4_2g26330 FvH4_2g26330
malus_domestica	MD15G1356500.v1.1
prunus_persica	Prupe.1G503700_v2.0.a1 Prupe.1G503700_v2.0.a1 Prupe.1G503700_v2.0.a1 Prupe.1G503700_v2.0.a1 Prupe.1G503700_v2.0.a1
pyrus_communis	pycom15g31860
rosa_chinensis	RchiOBHm_Chr6g0294911
rosa_laevigata	RLG00000011828 RLG00000019151
rosa_multiflora	Rmu_sc0000245.1_g000041 Rmu_sc0008966.1_g000001 Rmu_sc0021726.1_g000004 Rmu_sc0039793.1_g000001 Rmu_ssc0000227.1_g000001
rosa_roxburghii	Rroxscaffold_7G00172850
rosa_rugosa	Rorug06G0247700 Rorug06G0247800 Rorug06G0247900 Rorug06G0248000 Rorug06G0248000 Rorug06G0248100 Rorug06G0248200
rosa_samantha	Rh5BG206500 Rh5BG222800 Rh6AG361400 Rh6BG368800 Rh6CG375400 Rh6DG361800
rosa_wichuraiana	Rw6G031510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2060
AatII	GACGTC	1 cut(s)	1668
Acc16I	TGCGCA	2 cut(s)	324, 432
AccB7I	CCANNNNNTGG	1 cut(s)	568
AccI	GTMKAC	1 cut(s)	1957
AccII	CGCG	1 cut(s)	2283
AccIII	TCCGGA	2 cut(s)	126, 1279
AciI	CCGC	9 cut(s)	33, 38, 113, 140, 157, 233, 867, 1727, 2146
AclWI	GGATC	6 cut(s)	46, 881, 1040, 1053, 1271, 2060
AcsI	RAATTY	3 cut(s)	46, 1177, 1716
AcuI	CTGAAG	5 cut(s)	366, 804, 1137, 1493, 2396
AcyI	GRCGYC	1 cut(s)	1665
AfaI	GTAC	4 cut(s)	838, 1381, 1605, 1882
AfiI	CCNNNNNNNGG	2 cut(s)	568, 2140
AflII	CTTAAG	3 cut(s)	748, 1394, 2321
AflIII	ACRYGT	2 cut(s)	1133, 1893
AhdI	GACNNNNNGTC	1 cut(s)	1972
AjiI	CACGTC	1 cut(s)	2337
AjnI	CCWGG	6 cut(s)	66, 135, 561, 966, 2105, 2418
Alw21I	GWGCWC	2 cut(s)	207, 1761
Alw26I	GTCTC	7 cut(s)	489, 1647, 1672, 1902, 2101, 2352, 2361
Alw44I	GTGCAC	1 cut(s)	1757
AlwI	GGATC	6 cut(s)	46, 881, 1040, 1053, 1271, 2060
AlwNI	CAGNNNCTG	1 cut(s)	2317
Aor13HI	TCCGGA	2 cut(s)	126, 1279
AoxI	GGCC	3 cut(s)	141, 782, 2043
ApaLI	GTGCAC	1 cut(s)	1757
ApeKI	GCWGC	8 cut(s)	35, 281, 325, 1056, 1711, 1983, 2212, 2311
ApoI	RAATTY	3 cut(s)	46, 1177, 1716
AspLEI	GCGC	2 cut(s)	325, 433
AspS9I	GGNCC	9 cut(s)	529, 721, 782, 869, 1354, 2043, 2116, 2143, 2326
AsuHPI	GGTGA	3 cut(s)	601, 937, 1525
AvaII	GGWCC	7 cut(s)	529, 721, 869, 1354, 2116, 2143, 2326
BaeGI	GKGCMC	1 cut(s)	1761
BamHI	GGATCC	1 cut(s)	1045
BauI	CACGAG	1 cut(s)	2364
BbsI	GAAGAC	2 cut(s)	81, 96
Bbv12I	GWGCWC	2 cut(s)	207, 1761
BbvCI	CCTCAGC	1 cut(s)	2169
BbvI	GCAGC	8 cut(s)	22, 293, 337, 1043, 1723, 1970, 2224, 2323
BcgI	CGANNNNNNTGC	2 cut(s)	1910, 1944
BciT130I	CCWGG	6 cut(s)	68, 137, 563, 968, 2107, 2420
BciVI	GTATCC	2 cut(s)	232, 2157
BcoDI	GTCTC	7 cut(s)	489, 1647, 1672, 1902, 2101, 2352, 2361
BfaI	CTAG	1 cut(s)	1724
BfrI	CTTAAG	3 cut(s)	748, 1394, 2321
BfuI	GTATCC	2 cut(s)	232, 2157
Bme1390I	CCNGG	6 cut(s)	68, 137, 563, 968, 2107, 2420
Bme18I	GGWCC	7 cut(s)	529, 721, 869, 1354, 2116, 2143, 2326
BmeRI	GACNNNNNGTC	1 cut(s)	1972
BmgBI	CACGTC	1 cut(s)	2337
BmgT120I	GGNCC	9 cut(s)	529, 721, 782, 869, 1354, 2043, 2116, 2143, 2326
BmiI	GGNNCC	5 cut(s)	1047, 1164, 1552, 2144, 2328
BmrFI	CCNGG	6 cut(s)	68, 137, 563, 968, 2107, 2420
BmrI	ACTGGG	1 cut(s)	2195
BmuI	ACTGGG	1 cut(s)	2195
BpiI	GAAGAC	2 cut(s)	81, 96
BplI	GAGNNNNNCTC	4 cut(s)	1855, 1887, 2152, 2184
BpmI	CTGGAG	2 cut(s)	1884, 2402
Bpu10I	CCTNAGC	1 cut(s)	2169
BpuEI	CTTGAG	3 cut(s)	333, 888, 1564
BsaHI	GRCGYC	1 cut(s)	1665
BsaI	GGTCTC	2 cut(s)	2352, 2361
BsaJI	CCNNGG	4 cut(s)	191, 561, 966, 2105
BsaWI	WCCGGW	2 cut(s)	126, 1279
Bsc4I	CCNNNNNNNGG	2 cut(s)	568, 2140
Bse1I	ACTGG	8 cut(s)	23, 547, 724, 948, 1697, 1712, 2093, 2201
BseAI	TCCGGA	2 cut(s)	126, 1279
BseBI	CCWGG	6 cut(s)	68, 137, 563, 968, 2107, 2420
BseDI	CCNNGG	4 cut(s)	191, 561, 966, 2105
BseGI	GGATG	5 cut(s)	919, 1573, 1588, 1857, 1986
BseLI	CCNNNNNNNGG	2 cut(s)	568, 2140
BseMII	CTCAG	6 cut(s)	786, 1206, 1658, 1886, 2183, 2363
BseNI	ACTGG	8 cut(s)	23, 547, 724, 948, 1697, 1712, 2093, 2201
BseRI	GAGGAG	1 cut(s)	240
BseSI	GKGCMC	1 cut(s)	1761
BseXI	GCAGC	8 cut(s)	22, 293, 337, 1043, 1723, 1970, 2224, 2323
BseYI	CCCAGC	1 cut(s)	210
Bsh1236I	CGCG	1 cut(s)	2283
Bsh1285I	CGRYCG	1 cut(s)	2281
BshFI	GGCC	3 cut(s)	143, 784, 2045
BsiEI	CGRYCG	1 cut(s)	2281
BsiHKAI	GWGCWC	2 cut(s)	207, 1761
BsiSI	CCGG	3 cut(s)	127, 582, 1280
BslFI	GGGAC	2 cut(s)	487, 2156
BslI	CCNNNNNNNGG	2 cut(s)	568, 2140
BsmAI	GTCTC	7 cut(s)	489, 1647, 1672, 1902, 2101, 2352, 2361
BsmBI	CGTCTC	1 cut(s)	1672
BsmFI	GGGAC	2 cut(s)	487, 2156
BsnI	GGCC	3 cut(s)	143, 784, 2045
Bso31I	GGTCTC	2 cut(s)	2352, 2361
Bsp1286I	GDGCHC	2 cut(s)	207, 1761
Bsp13I	TCCGGA	2 cut(s)	126, 1279
Bsp1407I	TGTACA	1 cut(s)	1379
Bsp143I	GATC	8 cut(s)	51, 439, 599, 886, 1045, 1276, 2065, 2278
Bsp68I	TCGCGA	1 cut(s)	2283
BspACI	CCGC	9 cut(s)	33, 38, 113, 140, 157, 233, 867, 1727, 2146
BspANI	GGCC	3 cut(s)	143, 784, 2045
BspCNI	CTCAG	6 cut(s)	785, 1207, 1657, 1885, 2182, 2362
BspEI	TCCGGA	2 cut(s)	126, 1279
BspFNI	CGCG	1 cut(s)	2283
BspHI	TCATGA	1 cut(s)	2121
BspLI	GGNNCC	5 cut(s)	1047, 1164, 1552, 2144, 2328
BspPI	GGATC	6 cut(s)	46, 881, 1040, 1053, 1271, 2060
BspTI	CTTAAG	3 cut(s)	748, 1394, 2321
BspTNI	GGTCTC	2 cut(s)	2352, 2361
BsrGI	TGTACA	1 cut(s)	1379
BsrI	ACTGG	8 cut(s)	23, 547, 724, 948, 1697, 1712, 2093, 2201
BssECI	CCNNGG	4 cut(s)	191, 561, 966, 2105
BssMI	GATC	8 cut(s)	51, 439, 599, 886, 1045, 1276, 2065, 2278
BssNI	GRCGYC	1 cut(s)	1665
BssSI	CACGAG	1 cut(s)	2364
Bst2BI	CACGAG	1 cut(s)	2364
Bst2UI	CCWGG	6 cut(s)	68, 137, 563, 968, 2107, 2420
Bst4CI	ACNGT	2 cut(s)	1379, 2240
Bst6I	CTCTTC	1 cut(s)	1101
BstACI	GRCGYC	1 cut(s)	1665
BstAFI	CTTAAG	3 cut(s)	748, 1394, 2321
BstAPI	GCANNNNNTGC	1 cut(s)	2317
BstAUI	TGTACA	1 cut(s)	1379
BstC8I	GCNNGC	3 cut(s)	755, 759, 1776
BstF5I	GGATG	5 cut(s)	919, 1573, 1588, 1857, 1986
BstFNI	CGCG	1 cut(s)	2283
BstHHI	GCGC	2 cut(s)	325, 433
BstKTI	GATC	8 cut(s)	54, 442, 602, 889, 1048, 1279, 2068, 2281
BstMAI	GTCTC	7 cut(s)	489, 1647, 1672, 1902, 2101, 2352, 2361
BstMBI	GATC	8 cut(s)	51, 439, 599, 886, 1045, 1276, 2065, 2278
BstMCI	CGRYCG	1 cut(s)	2281
BstMWI	GCNNNNNNNGC	8 cut(s)	202, 211, 821, 971, 1414, 1645, 2221, 2317
BstNI	CCWGG	6 cut(s)	68, 137, 563, 968, 2107, 2420
BstNSI	RCATGY	4 cut(s)	1063, 1137, 1639, 1897
BstSCI	CCNGG	6 cut(s)	66, 135, 561, 966, 2105, 2418
BstSLI	GKGCMC	1 cut(s)	1761
BstUI	CGCG	1 cut(s)	2283
BstV1I	GCAGC	8 cut(s)	22, 293, 337, 1043, 1723, 1970, 2224, 2323
BstV2I	GAAGAC	2 cut(s)	81, 96
BstX2I	RGATCY	1 cut(s)	1045
BstYI	RGATCY	1 cut(s)	1045
BsuI	GTATCC	2 cut(s)	232, 2157
BsuRI	GGCC	3 cut(s)	143, 784, 2045
BtrI	CACGTC	1 cut(s)	2337
BtsCI	GGATG	5 cut(s)	919, 1573, 1588, 1857, 1986
BtsI	GCAGTG	3 cut(s)	1354, 1428, 1655
BtsIMutI	CAGTG	4 cut(s)	955, 1354, 1428, 1655
BtuMI	TCGCGA	1 cut(s)	2283
Cac8I	GCNNGC	3 cut(s)	755, 759, 1776
CaiI	CAGNNNCTG	1 cut(s)	2317
CciI	TCATGA	1 cut(s)	2121
CfoI	GCGC	2 cut(s)	325, 433
Cfr13I	GGNCC	9 cut(s)	529, 721, 782, 869, 1354, 2043, 2116, 2143, 2326
Csp6I	GTAC	4 cut(s)	837, 1380, 1604, 1881
CspCI	CAANNNNNGTGG	2 cut(s)	1417, 1452
CviQI	GTAC	4 cut(s)	837, 1380, 1604, 1881
DpnI	GATC	8 cut(s)	53, 441, 601, 888, 1047, 1278, 2067, 2280
DpnII	GATC	8 cut(s)	51, 439, 599, 886, 1045, 1276, 2065, 2278
DraI	TTTAAA	2 cut(s)	939, 1294
DriI	GACNNNNNGTC	1 cut(s)	1972
Eam1104I	CTCTTC	1 cut(s)	1101
Eam1105I	GACNNNNNGTC	1 cut(s)	1972
EarI	CTCTTC	1 cut(s)	1101
Eco31I	GGTCTC	2 cut(s)	2352, 2361
Eco47I	GGWCC	7 cut(s)	529, 721, 869, 1354, 2116, 2143, 2326
Eco57I	CTGAAG	5 cut(s)	366, 804, 1137, 1493, 2396
EcoO109I	RGGNCCY	1 cut(s)	2326
EcoRII	CCWGG	6 cut(s)	66, 135, 561, 966, 2105, 2418
EcoT22I	ATGCAT	2 cut(s)	361, 921
Esp3I	CGTCTC	1 cut(s)	1672
FaqI	GGGAC	2 cut(s)	487, 2156
FauI	CCCGC	2 cut(s)	40, 120
FauNDI	CATATG	1 cut(s)	2086
FblI	GTMKAC	1 cut(s)	1957
FokI	GGATG	5 cut(s)	906, 1580, 1595, 1844, 1973
FspBI	CTAG	1 cut(s)	1724
FspI	TGCGCA	2 cut(s)	324, 432
GlaI	GCGC	2 cut(s)	324, 432
GsaI	CCCAGC	1 cut(s)	214
GsuI	CTGGAG	2 cut(s)	1884, 2402
HaeIII	GGCC	3 cut(s)	143, 784, 2045
HapII	CCGG	3 cut(s)	127, 582, 1280
HhaI	GCGC	2 cut(s)	325, 433
Hin1I	GRCGYC	1 cut(s)	1665
Hin6I	GCGC	2 cut(s)	323, 431
HinP1I	GCGC	2 cut(s)	323, 431
HincII	GTYRAC	6 cut(s)	64, 960, 1113, 1300, 1732, 1958
HindII	GTYRAC	6 cut(s)	64, 960, 1113, 1300, 1732, 1958
HindIII	AAGCTT	1 cut(s)	930
HpaI	GTTAAC	1 cut(s)	1732
HpaII	CCGG	3 cut(s)	127, 582, 1280
HphI	GGTGA	3 cut(s)	601, 937, 1525
Hpy99I	CGWCG	1 cut(s)	97
HpyCH4III	ACNGT	2 cut(s)	1379, 2240
HpyCH4IV	ACGT	5 cut(s)	803, 1087, 1665, 1750, 2336
HpyF10VI	GCNNNNNNNGC	8 cut(s)	202, 211, 821, 971, 1414, 1645, 2221, 2317
HpySE526I	ACGT	5 cut(s)	803, 1087, 1665, 1750, 2336
Hsp92I	GRCGYC	1 cut(s)	1665
HspAI	GCGC	2 cut(s)	323, 431
Kpn2I	TCCGGA	2 cut(s)	126, 1279
KspAI	GTTAAC	1 cut(s)	1732
Kzo9I	GATC	8 cut(s)	51, 439, 599, 886, 1045, 1276, 2065, 2278
LmnI	GCTCC	6 cut(s)	210, 253, 1344, 1536, 1556, 2421
Lsp1109I	GCAGC	8 cut(s)	22, 293, 337, 1043, 1723, 1970, 2224, 2323
MaeI	CTAG	1 cut(s)	1724
MaeII	ACGT	5 cut(s)	803, 1087, 1665, 1750, 2336
MaeIII	GTNAC	7 cut(s)	607, 898, 943, 992, 1141, 1513, 2332
MalI	GATC	8 cut(s)	53, 441, 601, 888, 1047, 1278, 2067, 2280
MboI	GATC	8 cut(s)	51, 439, 599, 886, 1045, 1276, 2065, 2278
MboII	GAAGA	8 cut(s)	81, 96, 365, 678, 681, 1088, 1712, 2211
MfeI	CAATTG	3 cut(s)	318, 1002, 1050
MflI	RGATCY	1 cut(s)	1045
MhlI	GDGCHC	2 cut(s)	207, 1761
MlyI	GAGTC	5 cut(s)	301, 323, 509, 614, 1584
MmeI	TCCRAC	2 cut(s)	52, 2203
Mph1103I	ATGCAT	2 cut(s)	361, 921
MroI	TCCGGA	2 cut(s)	126, 1279
MseI	TTAA	8 cut(s)	416, 749, 938, 1023, 1293, 1395, 1731, 2322
MspA1I	CMGCKG	2 cut(s)	35, 605
MspCI	CTTAAG	3 cut(s)	748, 1394, 2321
MspI	CCGG	3 cut(s)	127, 582, 1280
MspR9I	CCNGG	6 cut(s)	68, 137, 563, 968, 2107, 2420
MunI	CAATTG	3 cut(s)	318, 1002, 1050
MvaI	CCWGG	6 cut(s)	68, 137, 563, 968, 2107, 2420
MvnI	CGCG	1 cut(s)	2283
MwoI	GCNNNNNNNGC	8 cut(s)	202, 211, 821, 971, 1414, 1645, 2221, 2317
NdeI	CATATG	1 cut(s)	2086
NdeII	GATC	8 cut(s)	51, 439, 599, 886, 1045, 1276, 2065, 2278
NlaIV	GGNNCC	5 cut(s)	1047, 1164, 1552, 2144, 2328
NmeAIII	GCCGAG	2 cut(s)	172, 1959
NmuCI	GTSAC	4 cut(s)	607, 943, 1513, 2332
NruI	TCGCGA	1 cut(s)	2283
NsbI	TGCGCA	2 cut(s)	324, 432
NsiI	ATGCAT	2 cut(s)	361, 921
NspI	RCATGY	4 cut(s)	1063, 1137, 1639, 1897
PagI	TCATGA	1 cut(s)	2121
PciI	ACATGT	2 cut(s)	1133, 1893
PcsI	WCGNNNNNNNCGW	2 cut(s)	92, 98
PfeI	GAWTC	6 cut(s)	71, 236, 260, 650, 1701, 1868
PflFI	GACNNNGTC	1 cut(s)	2330
PflMI	CCANNNNNTGG	1 cut(s)	568
PfoI	TCCNGGA	1 cut(s)	2418
Ple19I	CGATCG	1 cut(s)	2281
PleI	GAGTC	5 cut(s)	300, 322, 508, 614, 1583
PpsI	GAGTC	5 cut(s)	300, 322, 508, 614, 1583
PpuMI	RGGWCCY	1 cut(s)	2326
PscI	ACATGT	2 cut(s)	1133, 1893
PsiI	TTATAA	1 cut(s)	2060
Psp5II	RGGWCCY	1 cut(s)	2326
Psp6I	CCWGG	6 cut(s)	66, 135, 561, 966, 2105, 2418
PspFI	CCCAGC	1 cut(s)	210
PspGI	CCWGG	6 cut(s)	66, 135, 561, 966, 2105, 2418
PspN4I	GGNNCC	5 cut(s)	1047, 1164, 1552, 2144, 2328
PspPI	GGNCC	9 cut(s)	529, 721, 782, 869, 1354, 2043, 2116, 2143, 2326
PspPPI	RGGWCCY	1 cut(s)	2326
PstNI	CAGNNNCTG	1 cut(s)	2317
PsuI	RGATCY	1 cut(s)	1045
PsyI	GACNNNGTC	1 cut(s)	2330
PvuI	CGATCG	1 cut(s)	2281
PvuII	CAGCTG	1 cut(s)	605
RruI	TCGCGA	1 cut(s)	2283
RsaI	GTAC	4 cut(s)	838, 1381, 1605, 1882
RsaNI	GTAC	4 cut(s)	837, 1380, 1604, 1881
SalI	GTCGAC	1 cut(s)	1956
SaqAI	TTAA	8 cut(s)	416, 749, 938, 1023, 1293, 1395, 1731, 2322
Sau3AI	GATC	8 cut(s)	51, 439, 599, 886, 1045, 1276, 2065, 2278
Sau96I	GGNCC	9 cut(s)	529, 721, 782, 869, 1354, 2043, 2116, 2143, 2326
SchI	GAGTC	5 cut(s)	301, 323, 509, 614, 1584
ScrFI	CCNGG	6 cut(s)	68, 137, 563, 968, 2107, 2420
SduI	GDGCHC	2 cut(s)	207, 1761
SinI	GGWCC	7 cut(s)	529, 721, 869, 1354, 2116, 2143, 2326
SmlI	CTYRAG	6 cut(s)	348, 748, 903, 1394, 1543, 2321
SmoI	CTYRAG	6 cut(s)	348, 748, 903, 1394, 1543, 2321
SsiI	CCGC	9 cut(s)	33, 38, 113, 140, 157, 233, 867, 1727, 2146
SspI	AATATT	1 cut(s)	637
SspMI	CTAG	1 cut(s)	1724
StyD4I	CCNGG	6 cut(s)	66, 135, 561, 966, 2105, 2418
TaaI	ACNGT	2 cut(s)	1379, 2240
TaiI	ACGT	5 cut(s)	806, 1090, 1668, 1753, 2339
TaqI	TCGA	3 cut(s)	885, 1064, 1957
TaqII	GACCGA	1 cut(s)	49
TatI	WGTACW	2 cut(s)	836, 1379
TauI	GCSGC	2 cut(s)	41, 143
TfiI	GAWTC	6 cut(s)	71, 236, 260, 650, 1701, 1868
Tru1I	TTAA	8 cut(s)	416, 749, 938, 1023, 1293, 1395, 1731, 2322
Tru9I	TTAA	8 cut(s)	416, 749, 938, 1023, 1293, 1395, 1731, 2322
TscAI	CASTG	4 cut(s)	955, 1354, 1435, 1655
TseFI	GTSAC	4 cut(s)	607, 943, 1513, 2332
TseI	GCWGC	8 cut(s)	35, 281, 325, 1056, 1711, 1983, 2212, 2311
Tsp45I	GTSAC	4 cut(s)	607, 943, 1513, 2332
TspGWI	ACGGA	1 cut(s)	44
TspRI	CASTG	4 cut(s)	955, 1354, 1435, 1655
Tth111I	GACNNNGTC	1 cut(s)	2330
Van91I	CCANNNNNTGG	1 cut(s)	568
Vha464I	CTTAAG	3 cut(s)	748, 1394, 2321
VneI	GTGCAC	1 cut(s)	1757
VpaK11BI	GGWCC	7 cut(s)	529, 721, 869, 1354, 2116, 2143, 2326
XapI	RAATTY	3 cut(s)	46, 1177, 1716
XceI	RCATGY	4 cut(s)	1063, 1137, 1639, 1897
XcmI	CCANNNNNNNNNTGG	3 cut(s)	512, 539, 1968
XmiI	GTMKAC	1 cut(s)	1957
XspI	CTAG	1 cut(s)	1724
ZraI	GACGTC	1 cut(s)	1666
Zsp2I	ATGCAT	2 cut(s)	361, 921

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.