Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008190

Physical Location & Seq

Genomic Coordinates Reverse (-)

48160911 .. 48167140

Gene Structure 6230 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1389100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2157 bp

ATGTCGACCTTTCATGTCAGATTAGGCTGGGCACCAACCGGCAACGTGTTTTGGGCTTCCGAATCGACATGTCACTGTTTGGCAAAATTGTGCACCATCATTCTGTTCATCCAAGCCATGAATTTCCTAGCTGCTGCACAAAATAGTACAAGCGGCCTACAGTTGCATATCCGTGATAAAGAGGTGGTGGTGGAAAATGGGATTCTCCAGATGACGCTATCAAAACCCGGAGGGGTGGTCACCGGACTACAATATAACGGCGTCGACAATTTGCTTGAAGTTCTTAACGAAGCTCCCAATAGAGGGTACTGGGACCTGGTTTGGACCGCACCAGGGATTACAAGAAAGAAAGGAGCATTTGATAGGATTGAAGGAACTAATTTTTCAGTTATAGTGGAAACTGAGGACCAGATAGAGCTTTCATTCACCAGAATGTGGGATCCCTCTCTGGAGGGCAAGCTTGTTCCTTTAAACATAGACATAAGGTTTGTTATGCTTCGCAACTCGTCAGGCTTCTACACTTACGCTATTTATGAACACTTGAAGGAATGGCCTGCTTTCAACTTGACCAACACGAGGACAGTGTTTAAGCTCAGAAAAGAGAAGTTTCATTACATGGCAATATCAGACAAAAGGCAAAGATACATGCCACTTCCAGATGATCGGTTGCAAGGAAGAGGCCAAGCCTTGGCTTACCCAGAGGCAGTCTTGCTTGTCAATCCCGTGGAGCCACAGTTCAAAGGAGAAGTGGATGATAAGTATCTATACTCAATTGAGAACAAAGAAAATAGGGTTCATGGATGGATATCCACTGAACCACCCGTGGGATTCTGGCAGATTACTCCTAGTCAAGAGTTCAAATCTGGTGGGCCCTTCAAACAATGCCTTACTTCCCATGTTGGGCCAACCACCCTAGCTAAGAAAACATTCCACACAACTCTATTGGCACTGATCATCTGTTTGTCATGGAATCATCAGATATTTCATAGCACCCATTACTCCGGAGCGGAGTTGATAATAGAATTTAAGCCCAGTGAGCCATGGAAGAAAGTTCTTGGCCCCATTTTTATCTATCTTAATTCTTTGGTGACTGAAGATGACCCACTTCAGCAGCTATGGGAGGACGCTAAGCAGCAGATGAAAACTGAGGTTCAAAGCTGGCCATACGACTTCCCAAGTTCAGAAGACTTTCTATCATCGGATCAACGGGGTACTGTTACCGGCAGATTACTAGTACGTGACGGGTATATCAATACTGAAGACATATTCGGAGATGGTACACAGGTAGGGTTGGCAGCTCCAGGAGATGCTGGATCATGGCAACTAGAATGCAAGGGTTACCAATTTTGGACCAAAGCAAACGATAAAGGCTTCTTCTCTATAAGTGGAATACGTCCAGGGATCTATAATGTTTATGCATGGGTTCCTGGTTTTATTGGTGACTATCGACATGATGCTGAAGTAATTATAACTCCAGGTTGTAATGTTGATGTGGGTACACTTGTATATGAACCTCCAAGAAGTGGCCCAACTTTCTGGGAAATAGGCATCCCAGACCGAACTGCAGCAGAGTTTTATATTCCTGATCCTAATCCAAACCACATTAACAAACTATACGTCAATCACACTGACAGGTTTAGGCAATATGGATTATGGGAAAGATATGCAGAACTGTATCCATACAATGATTTGGTTTACACAGTGGGTGTTAATGATTATCGAAAAGATTTTTACTTCGCTCAAGTTACTAGGAAGACAGGTAATAACACATACCAAGGATCAACATGGCAGATTAGGTTCACACTTGACACTGTGGGCAAGAACAATACCTACACATTACGAATAGCACTGGCAACAGCACATGTTTCTGAATTACAAGTTCGGATCAACGACCTGGAAGCGAGCAATCCACTATTTTCCACAGGACAAATTGGAAACGACAATACGATAGCAAGGCATGGAATTCATGGGCTGTATAGGTTGTACACTGTCGACATTCCAGGTGCTCAGCTGCTGGAAGGGAACAATACCATTTTTCTAACTCGAGCATTGAGTATAAGTCCTTTCCAAGGAATTATGTACGACTATATTCGTTTAGAAGGTCCCGCTTCTTCCAACAACGAAAGTCTGGGATCCAGTTCTTGGGGATATAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

719

Amino Acids

81.49

Weight (kDa)

5.84

Isoelectric Point (pI)

31.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhamnogal_lyase	PF06045	44 - 250	9e-92	Rhamnogalacturonate lyase family
fn3_3	PF14686	425 - 497	1.1e-23	Polysaccharide lyase family 4, domain II
CBM-like	PF14683	511 - 699	9.6e-50	Polysaccharide lyase family 4, domain III

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008190)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g34130
malus_domestica	MD15G1389100.v1.1
prunus_persica	Prupe.1G537300_v2.0.a1 Prupe.1G537300_v2.0.a1 Prupe.1G537300_v2.0.a1 Prupe.1G537300_v2.0.a1
pyrus_communis	pycom15g34880 pycom15g34890
rosa_chinensis	RchiOBHm_Chr7g0233701
rosa_laevigata	RLG00000001241
rosa_multiflora	Rmu_co7977656.1_g000001 Rmu_sc0000556.1_g000027 Rmu_sc0005305.1_g000002 Rmu_sc0005305.1_g000003
rosa_roxburghii	Rroxscaffold_3G00227080
rosa_rugosa	Rorug07G0282100 Rorug07G0282200 Rorug07G0282200
rosa_samantha	Rh7AG438500 Rh7BG411200 Rh7CG458100 Rh7DG427500
rosa_wichuraiana	Rw7G036160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1469
AccB1I	GGYRCC	1 cut(s)	31
AccB7I	CCANNNNNTGG	4 cut(s)	435, 688, 1523, 2142
AccBSI	CCGCTC	1 cut(s)	1007
AccI	GTMKAC	3 cut(s)	5, 264, 1992
AccIII	TCCGGA	1 cut(s)	1001
AciI	CCGC	4 cut(s)	153, 327, 1007, 2106
AcoI	YGGCCR	1 cut(s)	1160
AcsI	RAATTY	3 cut(s)	121, 1022, 1962
AcuI	CTGAAG	4 cut(s)	1091, 1113, 1278, 1479
AcyI	GRCGYC	1 cut(s)	261
AfaI	GTAC	8 cut(s)	148, 308, 1213, 1236, 1279, 1498, 1985, 2081
AflIII	ACRYGT	3 cut(s)	45, 68, 1861
AgsI	TTSAA	8 cut(s)	278, 371, 544, 562, 739, 859, 877, 1154
AhlI	ACTAGT	1 cut(s)	1231
AjnI	CCWGG	8 cut(s)	315, 331, 1300, 1396, 1426, 1474, 1893, 1999
AloI	GAACNNNNNNTCC	2 cut(s)	719, 751
Alw21I	GWGCWC	2 cut(s)	95, 2008
Alw44I	GTGCAC	1 cut(s)	91
AlwNI	CAGNNNCTG	1 cut(s)	2014
Ama87I	CYCGRG	1 cut(s)	2043
Aor13HI	TCCGGA	1 cut(s)	1001
AoxI	GGCC	8 cut(s)	154, 551, 679, 869, 902, 1057, 1160, 1525
ApaI	GGGCCC	1 cut(s)	873
ApaLI	GTGCAC	1 cut(s)	91
ApeKI	GCWGC	7 cut(s)	131, 134, 1111, 1132, 1295, 1565, 2011
ApoI	RAATTY	3 cut(s)	121, 1022, 1962
ArsI	GACNNNNNNTTYG	4 cut(s)	470, 502, 1602, 1634
Asp700I	GAANNNNTTC	2 cut(s)	926, 1188
AsuC2I	CCSGG	1 cut(s)	228
AsuHPI	GGTGA	4 cut(s)	232, 418, 1099, 1451
AvaI	CYCGRG	1 cut(s)	2043
AvaII	GGWCC	5 cut(s)	313, 324, 406, 1350, 2102
BaeGI	GKGCMC	3 cut(s)	34, 95, 873
BaeI	ACNNNNGTAYC	4 cut(s)	1269, 1302, 1488, 1521
BalI	TGGCCA	1 cut(s)	1162
BamHI	GGATCC	2 cut(s)	439, 2132
BanI	GGYRCC	1 cut(s)	31
BanII	GRGCYC	1 cut(s)	873
BarI	GAAGNNNNNNTAC	2 cut(s)	596, 628
BauI	CACGAG	1 cut(s)	574
BbsI	GAAGAC	3 cut(s)	1191, 1266, 1760
Bbv12I	GWGCWC	2 cut(s)	95, 2008
BbvI	GCAGC	7 cut(s)	118, 121, 1123, 1144, 1307, 1577, 1998
BccI	CCATC	3 cut(s)	104, 795, 1268
BceAI	ACGGC	1 cut(s)	274
BciT130I	CCWGG	8 cut(s)	317, 333, 1302, 1398, 1428, 1476, 1895, 2001
BciVI	GTATCC	1 cut(s)	1686
BclI	TGATCA	1 cut(s)	951
BcnI	CCSGG	1 cut(s)	228
BcuI	ACTAGT	1 cut(s)	1231
BfaI	CTAG	6 cut(s)	128, 846, 914, 1232, 1325, 1749
BfmI	CTRYAG	2 cut(s)	158, 1563
BfuI	GTATCC	1 cut(s)	1686
BisI	GCNGC	8 cut(s)	132, 135, 154, 1112, 1133, 1296, 1566, 2012
BlpI	GCTNAGC	2 cut(s)	1128, 2007
BlsI	GCNGC	8 cut(s)	133, 136, 155, 1113, 1134, 1297, 1567, 2013
Bme1390I	CCNGG	9 cut(s)	228, 317, 333, 1302, 1398, 1428, 1476, 1895, 2001
Bme18I	GGWCC	5 cut(s)	313, 324, 406, 1350, 2102
BmeT110I	CYCGRG	1 cut(s)	2043
BmiI	GGNNCC	9 cut(s)	33, 314, 441, 729, 871, 1060, 1425, 2104, 2134
BmrFI	CCNGG	9 cut(s)	228, 317, 333, 1302, 1398, 1428, 1476, 1895, 2001
BmrI	ACTGGG	2 cut(s)	319, 1026
BmsI	GCATC	3 cut(s)	1297, 1444, 1557
BmuI	ACTGGG	2 cut(s)	319, 1026
BpiI	GAAGAC	3 cut(s)	1191, 1266, 1760
BpmI	CTGGAG	4 cut(s)	191, 470, 1284, 1458
Bpu1102I	GCTNAGC	2 cut(s)	1128, 2007
BpuEI	CTTGAG	1 cut(s)	1725
BpuMI	CCSGG	1 cut(s)	228
BsaAI	YACGTR	1 cut(s)	1238
BsaBI	GATNNNNATC	3 cut(s)	759, 805, 1590
BsaHI	GRCGYC	1 cut(s)	261
BsaJI	CCNNGG	8 cut(s)	332, 687, 723, 822, 1040, 1397, 1774, 2068
BsaWI	WCCGGW	2 cut(s)	242, 1001
BsaXI	ACNNNNNCTCC	4 cut(s)	222, 252, 719, 749
Bse118I	RCCGGY	2 cut(s)	38, 1220
Bse1I	ACTGG	4 cut(s)	314, 1032, 1854, 2136
Bse8I	GATNNNNATC	3 cut(s)	759, 805, 1590
BseAI	TCCGGA	1 cut(s)	1001
BseBI	CCWGG	8 cut(s)	317, 333, 1302, 1398, 1428, 1476, 1895, 2001
BseDI	CCNNGG	8 cut(s)	332, 687, 723, 822, 1040, 1397, 1774, 2068
BseGI	GGATG	4 cut(s)	108, 757, 806, 1548
BseJI	GATNNNNATC	3 cut(s)	759, 805, 1590
BseMII	CTCAG	4 cut(s)	393, 607, 1137, 2021
BseNI	ACTGG	4 cut(s)	314, 1032, 1854, 2136
BseSI	GKGCMC	3 cut(s)	34, 95, 873
BseXI	GCAGC	7 cut(s)	118, 121, 1123, 1144, 1307, 1577, 1998
BseYI	CCCAGC	1 cut(s)	27
BsgI	GTGCAG	1 cut(s)	120
BshFI	GGCC	8 cut(s)	156, 553, 681, 871, 904, 1059, 1162, 1527
BshNI	GGYRCC	1 cut(s)	31
BsiHKAI	GWGCWC	2 cut(s)	95, 2008
BsiHKCI	CYCGRG	1 cut(s)	2043
BsiSI	CCGG	5 cut(s)	39, 228, 243, 1002, 1221
BslFI	GGGAC	2 cut(s)	326, 2088
BsmFI	GGGAC	2 cut(s)	326, 2088
BsmI	GAATGC	1 cut(s)	1334
BsnI	GGCC	8 cut(s)	156, 553, 681, 871, 904, 1059, 1162, 1527
BsoBI	CYCGRG	1 cut(s)	2043
Bsp120I	GGGCCC	1 cut(s)	869
Bsp1286I	GDGCHC	4 cut(s)	34, 95, 873, 2008
Bsp13I	TCCGGA	1 cut(s)	1001
Bsp1407I	TGTACA	1 cut(s)	1983
Bsp1720I	GCTNAGC	2 cut(s)	1128, 2007
Bsp19I	CCATGG	1 cut(s)	1040
BspACI	CCGC	4 cut(s)	153, 327, 1007, 2106
BspANI	GGCC	8 cut(s)	156, 553, 681, 871, 904, 1059, 1162, 1527
BspCNI	CTCAG	4 cut(s)	394, 606, 1138, 2020
BspEI	TCCGGA	1 cut(s)	1001
BspLI	GGNNCC	9 cut(s)	33, 314, 441, 729, 871, 1060, 1425, 2104, 2134
BspMAI	CTGCAG	1 cut(s)	1567
BspT107I	GGYRCC	1 cut(s)	31
BsrBI	CCGCTC	1 cut(s)	1007
BsrFI	RCCGGY	2 cut(s)	38, 1220
BsrGI	TGTACA	1 cut(s)	1983
BsrI	ACTGG	4 cut(s)	314, 1032, 1854, 2136
BssAI	RCCGGY	2 cut(s)	38, 1220
BssECI	CCNNGG	8 cut(s)	332, 687, 723, 822, 1040, 1397, 1774, 2068
BssNI	GRCGYC	1 cut(s)	261
BssSI	CACGAG	1 cut(s)	574
BssT1I	CCWWGG	4 cut(s)	687, 1040, 1774, 2068
Bst2BI	CACGAG	1 cut(s)	574
Bst2UI	CCWGG	8 cut(s)	317, 333, 1302, 1398, 1428, 1476, 1895, 2001
Bst4CI	ACNGT	9 cut(s)	77, 162, 583, 735, 1216, 1674, 1702, 1813, 1990
Bst6I	CTCTTC	1 cut(s)	670
BstACI	GRCGYC	1 cut(s)	261
BstAUI	TGTACA	1 cut(s)	1983
BstBAI	YACGTR	1 cut(s)	1238
BstC8I	GCNNGC	4 cut(s)	458, 555, 1160, 1903
BstDEI	CTNAG	6 cut(s)	402, 593, 918, 1128, 1146, 2007
BstDSI	CCRYGG	3 cut(s)	723, 822, 1040
BstEII	GGTNACC	2 cut(s)	238, 1337
BstENI	CCTNNNNNAGG	1 cut(s)	2067
BstF5I	GGATG	4 cut(s)	108, 757, 806, 1548
BstMWI	GCNNNNNNNGC	1 cut(s)	1036
BstNI	CCWGG	8 cut(s)	317, 333, 1302, 1398, 1428, 1476, 1895, 2001
BstNSI	RCATGY	3 cut(s)	72, 649, 1865
BstPI	GGTNACC	2 cut(s)	238, 1337
BstSCI	CCNGG	9 cut(s)	226, 315, 331, 1300, 1396, 1426, 1474, 1893, 1999
BstSFI	CTRYAG	2 cut(s)	158, 1563
BstSLI	GKGCMC	3 cut(s)	34, 95, 873
BstV1I	GCAGC	7 cut(s)	118, 121, 1123, 1144, 1307, 1577, 1998
BstV2I	GAAGAC	3 cut(s)	1191, 1266, 1760
BstX2I	RGATCY	3 cut(s)	439, 1401, 2132
BstXI	CCANNNNNNTGG	1 cut(s)	1536
BstYI	RGATCY	3 cut(s)	439, 1401, 2132
BsuI	GTATCC	1 cut(s)	1686
BsuRI	GGCC	8 cut(s)	156, 553, 681, 871, 904, 1059, 1162, 1527
BtgI	CCRYGG	3 cut(s)	723, 822, 1040
BtsCI	GGATG	4 cut(s)	108, 757, 806, 1548
Cac8I	GCNNGC	4 cut(s)	458, 555, 1160, 1903
CaiI	CAGNNNCTG	1 cut(s)	2014
Cfr10I	RCCGGY	2 cut(s)	38, 1220
CseI	GACGC	3 cut(s)	223, 250, 1133
CsiI	ACCWGGT	1 cut(s)	315
Csp6I	GTAC	8 cut(s)	147, 307, 1212, 1235, 1278, 1497, 1984, 2080
CspCI	CAANNNNNGTGG	2 cut(s)	847, 882
CviQI	GTAC	8 cut(s)	147, 307, 1212, 1235, 1278, 1497, 1984, 2080
DdeI	CTNAG	6 cut(s)	402, 593, 918, 1128, 1146, 2007
DraI	TTTAAA	1 cut(s)	471
EaeI	YGGCCR	1 cut(s)	1160
Eam1104I	CTCTTC	1 cut(s)	670
EarI	CTCTTC	1 cut(s)	670
Eco130I	CCWWGG	4 cut(s)	687, 1040, 1774, 2068
Eco24I	GRGCYC	1 cut(s)	873
Eco32I	GATATC	1 cut(s)	807
Eco47I	GGWCC	5 cut(s)	313, 324, 406, 1350, 2102
Eco57I	CTGAAG	4 cut(s)	1091, 1113, 1278, 1479
Eco88I	CYCGRG	1 cut(s)	2043
Eco91I	GGTNACC	2 cut(s)	238, 1337
EcoNI	CCTNNNNNAGG	1 cut(s)	2067
EcoO109I	RGGNCCY	3 cut(s)	313, 870, 2102
EcoO65I	GGTNACC	2 cut(s)	238, 1337
EcoRI	GAATTC	1 cut(s)	1962
EcoRII	CCWGG	8 cut(s)	315, 331, 1300, 1396, 1426, 1474, 1893, 1999
EcoRV	GATATC	1 cut(s)	807
EcoT14I	CCWWGG	4 cut(s)	687, 1040, 1774, 2068
EcoT22I	ATGCAT	1 cut(s)	1420
EcoT38I	GRGCYC	1 cut(s)	873
ErhI	CCWWGG	4 cut(s)	687, 1040, 1774, 2068
FaqI	GGGAC	2 cut(s)	326, 2088
FauI	CCCGC	1 cut(s)	2113
FbaI	TGATCA	1 cut(s)	951
FblI	GTMKAC	3 cut(s)	5, 264, 1992
Fnu4HI	GCNGC	8 cut(s)	132, 135, 154, 1112, 1133, 1296, 1566, 2012
FokI	GGATG	4 cut(s)	95, 764, 813, 1535
FriOI	GRGCYC	1 cut(s)	873
Fsp4HI	GCNGC	8 cut(s)	132, 135, 154, 1112, 1133, 1296, 1566, 2012
FspBI	CTAG	6 cut(s)	128, 846, 914, 1232, 1325, 1749
GluI	GCNGC	8 cut(s)	132, 135, 154, 1112, 1133, 1296, 1566, 2012
GsaI	CCCAGC	1 cut(s)	31
GsuI	CTGGAG	4 cut(s)	191, 470, 1284, 1458
HaeIII	GGCC	8 cut(s)	156, 553, 681, 871, 904, 1059, 1162, 1527
HapII	CCGG	5 cut(s)	39, 228, 243, 1002, 1221
HgaI	GACGC	3 cut(s)	223, 250, 1133
Hin1I	GRCGYC	1 cut(s)	261
HincII	GTYRAC	3 cut(s)	6, 265, 1993
HindII	GTYRAC	3 cut(s)	6, 265, 1993
HindIII	AAGCTT	1 cut(s)	458
HinfI	GANTC	4 cut(s)	62, 202, 828, 970
HpaII	CCGG	5 cut(s)	39, 228, 243, 1002, 1221
HphI	GGTGA	4 cut(s)	232, 418, 1099, 1451
Hpy166II	GTNNAC	9 cut(s)	6, 93, 265, 1280, 1499, 1696, 1800, 1986, 1993
Hpy188III	TCNNGA	6 cut(s)	208, 449, 656, 851, 1002, 1583
Hpy8I	GTNNAC	9 cut(s)	6, 93, 265, 1280, 1499, 1696, 1800, 1986, 1993
Hpy99I	CGWCG	1 cut(s)	266
HpyAV	CCTTC	5 cut(s)	365, 538, 883, 2012, 2093
HpyCH4III	ACNGT	9 cut(s)	77, 162, 583, 735, 1216, 1674, 1702, 1813, 1990
HpyCH4IV	ACGT	4 cut(s)	45, 1237, 1393, 1617
HpyCH4V	TGCA	8 cut(s)	93, 137, 166, 670, 1332, 1418, 1565, 1667
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1036
HpyF3I	CTNAG	6 cut(s)	402, 593, 918, 1128, 1146, 2007
HpySE526I	ACGT	4 cut(s)	45, 1237, 1393, 1617
Hsp92I	GRCGYC	1 cut(s)	261
Kpn2I	TCCGGA	1 cut(s)	1001
Ksp22I	TGATCA	1 cut(s)	951
LmnI	GCTCC	5 cut(s)	298, 353, 727, 1004, 1303
Lsp1109I	GCAGC	7 cut(s)	118, 121, 1123, 1144, 1307, 1577, 1998
LweI	GCATC	3 cut(s)	1297, 1444, 1557
MabI	ACCWGGT	1 cut(s)	315
MaeI	CTAG	6 cut(s)	128, 846, 914, 1232, 1325, 1749
MaeII	ACGT	4 cut(s)	45, 1237, 1393, 1617
MaeIII	GTNAC	8 cut(s)	71, 238, 1087, 1216, 1238, 1337, 1439, 1744
MbiI	CCGCTC	1 cut(s)	1007
MboII	GAAGA	8 cut(s)	687, 1057, 1106, 1196, 1271, 1366, 1765, 2103
MfeI	CAATTG	1 cut(s)	771
MflI	RGATCY	3 cut(s)	439, 1401, 2132
MhlI	GDGCHC	4 cut(s)	34, 95, 873, 2008
MlsI	TGGCCA	1 cut(s)	1162
MluNI	TGGCCA	1 cut(s)	1162
MmeI	TCCRAC	1 cut(s)	2139
Mox20I	TGGCCA	1 cut(s)	1162
Mph1103I	ATGCAT	1 cut(s)	1420
MroI	TCCGGA	1 cut(s)	1001
MroXI	GAANNNNTTC	2 cut(s)	926, 1188
MscI	TGGCCA	1 cut(s)	1162
MseI	TTAA	7 cut(s)	285, 470, 588, 1026, 1077, 1605, 1710
MslI	CAYNNNNRTG	2 cut(s)	171, 431
Msp20I	TGGCCA	1 cut(s)	1162
MspA1I	CMGCKG	1 cut(s)	2011
MspI	CCGG	5 cut(s)	39, 228, 243, 1002, 1221
MspR9I	CCNGG	9 cut(s)	228, 317, 333, 1302, 1398, 1428, 1476, 1895, 2001
MunI	CAATTG	1 cut(s)	771
Mva1269I	GAATGC	1 cut(s)	1334
MvaI	CCWGG	8 cut(s)	317, 333, 1302, 1398, 1428, 1476, 1895, 2001
MwoI	GCNNNNNNNGC	1 cut(s)	1036
NciI	CCSGG	1 cut(s)	228
NcoI	CCATGG	1 cut(s)	1040
NlaIV	GGNNCC	9 cut(s)	33, 314, 441, 729, 871, 1060, 1425, 2104, 2134
NmuCI	GTSAC	5 cut(s)	71, 238, 1087, 1238, 1439
NsiI	ATGCAT	1 cut(s)	1420
NspI	RCATGY	3 cut(s)	72, 649, 1865
PaeR7I	CTCGAG	1 cut(s)	2043
PciI	ACATGT	2 cut(s)	68, 1861
PctI	GAATGC	1 cut(s)	1334
PdmI	GAANNNNTTC	2 cut(s)	926, 1188
PfeI	GAWTC	4 cut(s)	62, 202, 828, 970
PflMI	CCANNNNNTGG	4 cut(s)	435, 688, 1523, 2142
PfoI	TCCNGGA	1 cut(s)	1300
PkrI	GCNGC	8 cut(s)	133, 136, 155, 1113, 1134, 1297, 1567, 2013
Ppu21I	YACGTR	1 cut(s)	1238
PpuMI	RGGWCCY	2 cut(s)	313, 2102
PscI	ACATGT	2 cut(s)	68, 1861
PsiI	TTATAA	1 cut(s)	1469
Psp5II	RGGWCCY	2 cut(s)	313, 2102
Psp6I	CCWGG	8 cut(s)	315, 331, 1300, 1396, 1426, 1474, 1893, 1999
PspEI	GGTNACC	2 cut(s)	238, 1337
PspFI	CCCAGC	1 cut(s)	27
PspGI	CCWGG	8 cut(s)	315, 331, 1300, 1396, 1426, 1474, 1893, 1999
PspN4I	GGNNCC	9 cut(s)	33, 314, 441, 729, 871, 1060, 1425, 2104, 2134
PspOMI	GGGCCC	1 cut(s)	869
PspPPI	RGGWCCY	2 cut(s)	313, 2102
PspXI	VCTCGAGB	1 cut(s)	2043
PsrI	GAACNNNNNNTAC	2 cut(s)	1814, 1846
PstI	CTGCAG	1 cut(s)	1567
PstNI	CAGNNNCTG	1 cut(s)	2014
PsuI	RGATCY	3 cut(s)	439, 1401, 2132
PvuII	CAGCTG	1 cut(s)	2011
RsaI	GTAC	8 cut(s)	148, 308, 1213, 1236, 1279, 1498, 1985, 2081
RsaNI	GTAC	8 cut(s)	147, 307, 1212, 1235, 1278, 1497, 1984, 2080
RseI	CAYNNNNRTG	2 cut(s)	171, 431
SalI	GTCGAC	3 cut(s)	4, 263, 1991
SaqAI	TTAA	7 cut(s)	285, 470, 588, 1026, 1077, 1605, 1710
SatI	GCNGC	8 cut(s)	132, 135, 154, 1112, 1133, 1296, 1566, 2012
ScrFI	CCNGG	9 cut(s)	228, 317, 333, 1302, 1398, 1428, 1476, 1895, 2001
SduI	GDGCHC	4 cut(s)	34, 95, 873, 2008
SexAI	ACCWGGT	1 cut(s)	315
SfaNI	GCATC	3 cut(s)	1297, 1444, 1557
SfcI	CTRYAG	2 cut(s)	158, 1563
Sfr274I	CTCGAG	1 cut(s)	2043
SinI	GGWCC	5 cut(s)	313, 324, 406, 1350, 2102
SlaI	CTCGAG	1 cut(s)	2043
SmiMI	CAYNNNNRTG	2 cut(s)	171, 431
SmlI	CTYRAG	2 cut(s)	1740, 2043
SmoI	CTYRAG	2 cut(s)	1740, 2043
SpeI	ACTAGT	1 cut(s)	1231
SsiI	CCGC	4 cut(s)	153, 327, 1007, 2106
SspMI	CTAG	6 cut(s)	128, 846, 914, 1232, 1325, 1749
StyD4I	CCNGG	9 cut(s)	226, 315, 331, 1300, 1396, 1426, 1474, 1893, 1999
StyI	CCWWGG	4 cut(s)	687, 1040, 1774, 2068
TaaI	ACNGT	9 cut(s)	77, 162, 583, 735, 1216, 1674, 1702, 1813, 1990
TaiI	ACGT	4 cut(s)	48, 1240, 1396, 1620
TaqI	TCGA	7 cut(s)	5, 65, 264, 1447, 1720, 1992, 2044
TaqII	GACCGA	1 cut(s)	1572
TatI	WGTACW	2 cut(s)	146, 1983
TauI	GCSGC	1 cut(s)	156
TfiI	GAWTC	4 cut(s)	62, 202, 828, 970
Tru1I	TTAA	7 cut(s)	285, 470, 588, 1026, 1077, 1605, 1710
Tru9I	TTAA	7 cut(s)	285, 470, 588, 1026, 1077, 1605, 1710
TseFI	GTSAC	5 cut(s)	71, 238, 1087, 1238, 1439
TseI	GCWGC	7 cut(s)	131, 134, 1111, 1132, 1295, 1565, 2011
Tsp45I	GTSAC	5 cut(s)	71, 238, 1087, 1238, 1439
TspGWI	ACGGA	1 cut(s)	161
Van91I	CCANNNNNTGG	4 cut(s)	435, 688, 1523, 2142
VneI	GTGCAC	1 cut(s)	91
VpaK11BI	GGWCC	5 cut(s)	313, 324, 406, 1350, 2102
XagI	CCTNNNNNAGG	1 cut(s)	2067
XapI	RAATTY	3 cut(s)	121, 1022, 1962
XceI	RCATGY	3 cut(s)	72, 649, 1865
XhoI	CTCGAG	1 cut(s)	2043
XmiI	GTMKAC	3 cut(s)	5, 264, 1992
XmnI	GAANNNNTTC	2 cut(s)	926, 1188
XspI	CTAG	6 cut(s)	128, 846, 914, 1232, 1325, 1749
Zsp2I	ATGCAT	1 cut(s)	1420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.