RchiOBHm_Chr7g0233701

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008190
Physical Location & Seq
Forward (+)
58201061 .. 58205930
4870 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20947

Sequence Viewer

Length: 1671 bp
ATGGTGGTCATGGATAATGGCATTCTCCAAGTCAATCTTTCAAAACCAGAGGGGATGGTTACTGGAATACAATATAAAGGCATCGACAATCTGCTTGAAGTTCTTGAAGATGAAACCAATAGAGGGTACTGGGATATGGTTTGGTCTGCACCAGGAATTACGAGAAAGAAAGGCGCATTTGATAGGTTTCATTACATGGCAATATCAGACAGCAGGCAAAGACACATGCCCCTTCCCGATGATCGATCAGAAAAAAGAGGGCAAGCCCTGGCTTACCCGGAGGCGGTCTTGCTCGTCAATCCGGTCGAGCCAGAGTTCAAAGGAGAGGTGGATGACAAATATCAATATTCGGTTGAGAACATACATAATCGGGTGCATGGATGGATAGCAAATGACTCACAGGTTGGATTTTGGCAGATTACTCCCAGCGAAGAGTTTAAATCTGGAGGTCCCTTCAAGCAGTGCCTTACTTCACATGTGGGACCCATCACCCTTGCCATCTTTCATAGTACCCATTACTCAGGAGGGGAACTGATATTACAATTTGGGTCCAATGAGCCATGGAAGAAAGTGTTTGGCCCAGTTTTTTTCTATCTTAATTCTTTGGGTGATGGAGCTAACCCTTTCCAGTGTCTATGGGAGGATGCTAAGCAACAGATGAACGATGAAGTTGAAAGGTGGCCATATGATTTTCCAGCTTCAAAGGACTTTCTGTCGCCTAAGCAACGAGGAACAGTTAGCGGCAGACTATTGGTCCGCGAAAGGTATGTTACGGATGATAAAGACGTACCGGGAAATGGAACTCTGGTAGGCTTGGCAGCTCCAGGAGTTGTTGGATCATGGCAGTTAGAATGCAAGGGTTACCAATTCTGGACCAAAGCTGATAATGATGGCTACTTCTCTATTCATGGCGTACGCCCAGGGACCTATAATCTTTATGCATGGGTTCCTGGTTTTATTGGTGATTACCGAGATGTTTCTGAAATTGTTGTAACTCCAGATGGTGATGTTGATGTGGGTACACTTGTATATGAGCCTCCGCGAACTGGCCCAACTCTGTGGGAAATAGGCGTCCCCGATCGAACTGCAGCAGAATTTTACGTCCCAGATCCTAATCCAAAGTACATTAACAAACTCTACGTCAACACTGACAGGTTTAGACAATATGGATTGTGGGAAAGATATGCAGAGTTATATCCTGACAATGACTTGGTTTACACAATTGGAGTTAGCGATTATAGAAAGGATTTCTTCTTTGCTCAGGTTACCAGGAAGACAGGTAATAACACATACAAAGGATCAACATGGCAGATTAGGTTTAACCTAGACAACGTGGACAAGAACACTACCTACAAACTACGAGTATCACTTGCAACAGCACATGTTTCTGAATTACAAGTTCGGATCAATGACCCCGATTCAGAGAATGATCCTCTATTTACTACCGGACAGATTGGGAACGACAACACGATCGCAAGGCATGGAATTCATGGACTGTACAGGCTATACAATGTGGTCATTCCAAGCGCTCAGCTAGTCGACGGGAACAATACCATATTTCTAACTCGAGAATTGAGTGTCAGCCCCTTTCAGGGAATCATGTATGATTATATTCGTTTAGAAGGCCCCCAATCTTCTAATGACAACAATAATATTGGATTTAGTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

556

Amino Acids

63.09

Weight (kDa)

5.12

Isoelectric Point (pI)

30.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhamnogal_lyase PF06045 2 - 55 3.6e-21 Rhamnogalacturonate lyase family
Rhamnogal_lyase PF06045 61 - 110 1.3e-19 Rhamnogalacturonate lyase family
fn3_3 PF14686 266 - 335 5.3e-25 Polysaccharide lyase family 4, domain II
CBM-like PF14683 352 - 540 3.1e-51 Polysaccharide lyase family 4, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1539
AccII CGCG 2 cut(s) 759, 1042
AciI CCGC 4 cut(s) 284, 741, 757, 1040
AclWI GGATC 5 cut(s) 844, 1103, 1306, 1412, 1424
AcoI YGGCCR 1 cut(s) 680
AcsI RAATTY 2 cut(s) 1094, 1485
AcyI GRCGYC 1 cut(s) 1071
AfaI GTAC 7 cut(s) 128, 511, 789, 915, 1021, 1124, 1499
AfeI AGCGCT 1 cut(s) 1528
AfiI CCNNNNNNNGG 6 cut(s) 123, 283, 797, 1046, 1591, 1592
AflIII ACRYGT 2 cut(s) 475, 1381
AgsI TTSAA 7 cut(s) 42, 98, 107, 319, 457, 674, 702
AhdI GACNNNNNGTC 2 cut(s) 712, 752
AjnI CCWGG 6 cut(s) 151, 267, 823, 919, 949, 1268
AluBI AGCT 5 cut(s) 617, 698, 821, 881, 1534
AluI AGCT 5 cut(s) 617, 698, 821, 881, 1534
AlwI GGATC 5 cut(s) 844, 1103, 1306, 1412, 1424
Ama87I CYCGRG 1 cut(s) 1566
Aor51HI AGCGCT 1 cut(s) 1528
AoxI GGCC 4 cut(s) 577, 680, 1048, 1624
ApeKI GCWGC 2 cut(s) 818, 1088
ApoI RAATTY 2 cut(s) 1094, 1485
ArsI GACNNNNNNTTYG 2 cut(s) 1125, 1157
AspLEI GCGC 2 cut(s) 176, 1529
AspS9I GGNCC 9 cut(s) 449, 482, 549, 578, 754, 873, 924, 1049, 1625
AsuC2I CCSGG 2 cut(s) 278, 792
AsuHPI GGTGA 4 cut(s) 481, 620, 974, 1016
AvaI CYCGRG 1 cut(s) 1566
AvaII GGWCC 6 cut(s) 449, 482, 549, 754, 873, 924
BaeI ACNNNNGTAYC 2 cut(s) 1011, 1044
BalI TGGCCA 1 cut(s) 682
BbsI GAAGAC 1 cut(s) 1280
BbvI GCAGC 2 cut(s) 830, 1100
BccI CCATC 7 cut(s) 49, 375, 494, 506, 605, 884, 995
BcgI CGANNNNNNTGC 2 cut(s) 1067, 1101
BciT130I CCWGG 6 cut(s) 153, 269, 825, 921, 951, 1270
BcnI CCSGG 2 cut(s) 278, 792
BfaI CTAG 2 cut(s) 1325, 1535
BfmI CTRYAG 1 cut(s) 1086
BfoI RGCGCY 1 cut(s) 1530
BisI GCNGC 3 cut(s) 742, 819, 1089
BlpI GCTNAGC 2 cut(s) 648, 1530
BlsI GCNGC 3 cut(s) 743, 820, 1090
Bme1390I CCNGG 8 cut(s) 153, 269, 278, 792, 825, 921, 951, 1270
Bme18I GGWCC 6 cut(s) 449, 482, 549, 754, 873, 924
BmeRI GACNNNNNGTC 2 cut(s) 712, 752
BmeT110I CYCGRG 1 cut(s) 1566
BmgT120I GGNCC 9 cut(s) 449, 482, 549, 578, 754, 873, 924, 1049, 1625
BmiI GGNNCC 7 cut(s) 451, 483, 484, 550, 925, 948, 1627
BmrFI CCNGG 8 cut(s) 153, 269, 278, 792, 825, 921, 951, 1270
BmrI ACTGGG 2 cut(s) 139, 575
BmsI GCATC 2 cut(s) 90, 634
BmuI ACTGGG 2 cut(s) 139, 575
BpiI GAAGAC 1 cut(s) 1280
BpmI CTGGAG 3 cut(s) 465, 807, 981
Bpu10I CCTNAGC 2 cut(s) 720, 1260
Bpu1102I GCTNAGC 2 cut(s) 648, 1530
BpuMI CCSGG 2 cut(s) 278, 792
Bsa29I ATCGAT 1 cut(s) 244
BsaBI GATNNNNATC 1 cut(s) 1113
BsaHI GRCGYC 1 cut(s) 1071
BsaJI CCNNGG 4 cut(s) 267, 560, 919, 920
BsaWI WCCGGW 2 cut(s) 301, 1445
Bsc4I CCNNNNNNNGG 6 cut(s) 123, 283, 797, 1046, 1591, 1592
Bse1I ACTGG 5 cut(s) 67, 134, 581, 628, 1051
Bse8I GATNNNNATC 1 cut(s) 1113
BseBI CCWGG 6 cut(s) 153, 269, 825, 921, 951, 1270
BseCI ATCGAT 1 cut(s) 244
BseDI CCNNGG 4 cut(s) 267, 560, 919, 920
BseGI GGATG 5 cut(s) 60, 337, 386, 649, 781
BseJI GATNNNNATC 1 cut(s) 1113
BseLI CCNNNNNNNGG 6 cut(s) 123, 283, 797, 1046, 1591, 1592
BseMII CTCAG 3 cut(s) 534, 1274, 1544
BseNI ACTGG 5 cut(s) 67, 134, 581, 628, 1051
BseXI GCAGC 2 cut(s) 830, 1100
BseYI CCCAGC 1 cut(s) 425
BsgI GTGCAG 1 cut(s) 132
Bsh1236I CGCG 2 cut(s) 759, 1042
Bsh1285I CGRYCG 3 cut(s) 306, 1081, 1473
BshFI GGCC 4 cut(s) 579, 682, 1050, 1626
BshVI ATCGAT 1 cut(s) 244
BsiEI CGRYCG 3 cut(s) 306, 1081, 1473
BsiHKCI CYCGRG 1 cut(s) 1566
BsiSI CCGG 4 cut(s) 278, 302, 791, 1446
BsiWI CGTACG 1 cut(s) 913
BslFI GGGAC 5 cut(s) 435, 495, 937, 1058, 1088
BslI CCNNNNNNNGG 6 cut(s) 123, 283, 797, 1046, 1591, 1592
BsmFI GGGAC 5 cut(s) 435, 495, 937, 1058, 1088
BsmI GAATGC 2 cut(s) 21, 857
BsnI GGCC 4 cut(s) 579, 682, 1050, 1626
BsoBI CYCGRG 1 cut(s) 1566
Bsp1407I TGTACA 1 cut(s) 1497
Bsp143I GATC 9 cut(s) 241, 245, 836, 1078, 1108, 1298, 1404, 1429, 1470
Bsp1720I GCTNAGC 2 cut(s) 648, 1530
Bsp19I CCATGG 1 cut(s) 560
BspACI CCGC 4 cut(s) 284, 741, 757, 1040
BspANI GGCC 4 cut(s) 579, 682, 1050, 1626
BspCNI CTCAG 3 cut(s) 533, 1273, 1543
BspDI ATCGAT 1 cut(s) 244
BspFNI CGCG 2 cut(s) 759, 1042
BspLI GGNNCC 7 cut(s) 451, 483, 484, 550, 925, 948, 1627
BspMAI CTGCAG 1 cut(s) 1090
BspPI GGATC 5 cut(s) 844, 1103, 1306, 1412, 1424
BsrGI TGTACA 1 cut(s) 1497
BsrI ACTGG 5 cut(s) 67, 134, 581, 628, 1051
BssECI CCNNGG 4 cut(s) 267, 560, 919, 920
BssMI GATC 9 cut(s) 241, 245, 836, 1078, 1108, 1298, 1404, 1429, 1470
BssNI GRCGYC 1 cut(s) 1071
BssT1I CCWWGG 1 cut(s) 560
Bst2UI CCWGG 6 cut(s) 153, 269, 825, 921, 951, 1270
Bst4CI ACNGT 2 cut(s) 736, 1497
Bst6I CTCTTC 1 cut(s) 426
BstACI GRCGYC 1 cut(s) 1071
BstAUI TGTACA 1 cut(s) 1497
BstC8I GCNNGC 2 cut(s) 215, 264
BstDEI CTNAG 5 cut(s) 520, 648, 720, 1260, 1530
BstDSI CCRYGG 1 cut(s) 560
BstEII GGTNACC 2 cut(s) 860, 1264
BstF5I GGATG 5 cut(s) 60, 337, 386, 649, 781
BstFNI CGCG 2 cut(s) 759, 1042
BstH2I RGCGCY 1 cut(s) 1530
BstHHI GCGC 2 cut(s) 176, 1529
BstKTI GATC 9 cut(s) 244, 248, 839, 1081, 1111, 1301, 1407, 1432, 1473
BstMBI GATC 9 cut(s) 241, 245, 836, 1078, 1108, 1298, 1404, 1429, 1470
BstMCI CGRYCG 3 cut(s) 306, 1081, 1473
BstNI CCWGG 6 cut(s) 153, 269, 825, 921, 951, 1270
BstNSI RCATGY 3 cut(s) 229, 479, 1385
BstPI GGTNACC 2 cut(s) 860, 1264
BstSCI CCNGG 8 cut(s) 151, 267, 276, 790, 823, 919, 949, 1268
BstSFI CTRYAG 1 cut(s) 1086
BstUI CGCG 2 cut(s) 759, 1042
BstV1I GCAGC 2 cut(s) 830, 1100
BstV2I GAAGAC 1 cut(s) 1280
BstX2I RGATCY 1 cut(s) 1108
BstXI CCANNNNNNTGG 1 cut(s) 1059
BstYI RGATCY 1 cut(s) 1108
Bsu15I ATCGAT 1 cut(s) 244
BsuRI GGCC 4 cut(s) 579, 682, 1050, 1626
BsuTUI ATCGAT 1 cut(s) 244
BtgI CCRYGG 1 cut(s) 560
BtsCI GGATG 5 cut(s) 60, 337, 386, 649, 781
BtsI GCAGTG 1 cut(s) 467
BtsIMutI CAGTG 3 cut(s) 467, 635, 1146
Cac8I GCNNGC 2 cut(s) 215, 264
CfoI GCGC 2 cut(s) 176, 1529
Cfr13I GGNCC 9 cut(s) 449, 482, 549, 578, 754, 873, 924, 1049, 1625
ClaI ATCGAT 1 cut(s) 244
CseI GACGC 1 cut(s) 1060
Csp6I GTAC 7 cut(s) 127, 510, 788, 914, 1020, 1123, 1498
CviQI GTAC 7 cut(s) 127, 510, 788, 914, 1020, 1123, 1498
DdeI CTNAG 5 cut(s) 520, 648, 720, 1260, 1530
DpnI GATC 9 cut(s) 243, 247, 838, 1080, 1110, 1300, 1406, 1431, 1472
DpnII GATC 9 cut(s) 241, 245, 836, 1078, 1108, 1298, 1404, 1429, 1470
DraI TTTAAA 1 cut(s) 439
DriI GACNNNNNGTC 2 cut(s) 712, 752
EaeI YGGCCR 1 cut(s) 680
Eam1104I CTCTTC 1 cut(s) 426
Eam1105I GACNNNNNGTC 2 cut(s) 712, 752
EarI CTCTTC 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 560
Eco47I GGWCC 6 cut(s) 449, 482, 549, 754, 873, 924
Eco47III AGCGCT 1 cut(s) 1528
Eco88I CYCGRG 1 cut(s) 1566
Eco91I GGTNACC 2 cut(s) 860, 1264
EcoO109I RGGNCCY 4 cut(s) 449, 482, 924, 1625
EcoO65I GGTNACC 2 cut(s) 860, 1264
EcoRI GAATTC 1 cut(s) 1485
EcoRII CCWGG 6 cut(s) 151, 267, 823, 919, 949, 1268
EcoT14I CCWWGG 1 cut(s) 560
EcoT22I ATGCAT 1 cut(s) 943
ErhI CCWWGG 1 cut(s) 560
FalI AAGNNNNNCTT 4 cut(s) 21, 53, 1235, 1267
FaqI GGGAC 5 cut(s) 435, 495, 937, 1058, 1088
FauNDI CATATG 1 cut(s) 685
FblI GTMKAC 1 cut(s) 1539
Fnu4HI GCNGC 3 cut(s) 742, 819, 1089
FokI GGATG 5 cut(s) 67, 344, 393, 656, 788
Fsp4HI GCNGC 3 cut(s) 742, 819, 1089
FspBI CTAG 2 cut(s) 1325, 1535
GlaI GCGC 2 cut(s) 175, 1528
GluI GCNGC 3 cut(s) 742, 819, 1089
GsaI CCCAGC 1 cut(s) 429
GsuI CTGGAG 3 cut(s) 465, 807, 981
HaeII RGCGCY 1 cut(s) 1530
HaeIII GGCC 4 cut(s) 579, 682, 1050, 1626
HapII CCGG 4 cut(s) 278, 302, 791, 1446
HgaI GACGC 1 cut(s) 1060
HhaI GCGC 2 cut(s) 176, 1529
Hin1I GRCGYC 1 cut(s) 1071
Hin6I GCGC 2 cut(s) 174, 1527
HinP1I GCGC 2 cut(s) 174, 1527
HincII GTYRAC 2 cut(s) 1144, 1540
HindII GTYRAC 2 cut(s) 1144, 1540
HinfI GANTC 3 cut(s) 395, 1418, 1596
HpaII CCGG 4 cut(s) 278, 302, 791, 1446
HphI GGTGA 4 cut(s) 481, 620, 974, 1016
Hpy166II GTNNAC 5 cut(s) 1022, 1144, 1216, 1336, 1540
Hpy188I TCNGA 6 cut(s) 208, 250, 982, 1390, 1404, 1423
Hpy188III TCNNGA 8 cut(s) 104, 236, 444, 522, 871, 998, 1199, 1568
Hpy8I GTNNAC 5 cut(s) 1022, 1144, 1216, 1336, 1540
Hpy99I CGWCG 1 cut(s) 1544
HpyAV CCTTC 3 cut(s) 242, 463, 1616
HpyCH4III ACNGT 2 cut(s) 736, 1497
HpyCH4IV ACGT 4 cut(s) 786, 1101, 1140, 1332
HpyCH4V TGCA 7 cut(s) 149, 376, 855, 941, 1088, 1187, 1373
HpyF3I CTNAG 5 cut(s) 520, 648, 720, 1260, 1530
HpySE526I ACGT 4 cut(s) 786, 1101, 1140, 1332
Hsp92I GRCGYC 1 cut(s) 1071
HspAI GCGC 2 cut(s) 174, 1527
KflI GGGWCCC 1 cut(s) 482
Kzo9I GATC 9 cut(s) 241, 245, 836, 1078, 1108, 1298, 1404, 1429, 1470
LmnI GCTCC 2 cut(s) 614, 826
Lsp1109I GCAGC 2 cut(s) 830, 1100
LweI GCATC 2 cut(s) 90, 634
MaeI CTAG 2 cut(s) 1325, 1535
MaeII ACGT 4 cut(s) 786, 1101, 1140, 1332
MaeIII GTNAC 5 cut(s) 58, 769, 860, 991, 1264
MalI GATC 9 cut(s) 243, 247, 838, 1080, 1110, 1300, 1406, 1431, 1472
MboI GATC 9 cut(s) 241, 245, 836, 1078, 1108, 1298, 1404, 1429, 1470
MboII GAAGA 6 cut(s) 119, 443, 577, 1243, 1285, 1626
MfeI CAATTG 1 cut(s) 1221
MflI RGATCY 1 cut(s) 1108
MlsI TGGCCA 1 cut(s) 682
MluNI TGGCCA 1 cut(s) 682
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 2 cut(s) 385, 814
Mox20I TGGCCA 1 cut(s) 682
Mph1103I ATGCAT 1 cut(s) 943
MscI TGGCCA 1 cut(s) 682
MseI TTAA 4 cut(s) 438, 597, 1128, 1320
Msp20I TGGCCA 1 cut(s) 682
MspI CCGG 4 cut(s) 278, 302, 791, 1446
MspR9I CCNGG 8 cut(s) 153, 269, 278, 792, 825, 921, 951, 1270
MunI CAATTG 1 cut(s) 1221
Mva1269I GAATGC 2 cut(s) 21, 857
MvaI CCWGG 6 cut(s) 153, 269, 825, 921, 951, 1270
MvnI CGCG 2 cut(s) 759, 1042
NciI CCSGG 2 cut(s) 278, 792
NcoI CCATGG 1 cut(s) 560
NdeI CATATG 1 cut(s) 685
NdeII GATC 9 cut(s) 241, 245, 836, 1078, 1108, 1298, 1404, 1429, 1470
NlaIV GGNNCC 7 cut(s) 451, 483, 484, 550, 925, 948, 1627
NsiI ATGCAT 1 cut(s) 943
NspI RCATGY 3 cut(s) 229, 479, 1385
PaeR7I CTCGAG 1 cut(s) 1566
PasI CCCWGGG 1 cut(s) 920
PciI ACATGT 2 cut(s) 475, 1381
PctI GAATGC 2 cut(s) 21, 857
PfeI GAWTC 2 cut(s) 1418, 1596
Pfl23II CGTACG 1 cut(s) 913
PfoI TCCNGGA 1 cut(s) 823
PkrI GCNGC 3 cut(s) 743, 820, 1090
Ple19I CGATCG 2 cut(s) 1081, 1473
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
PpuMI RGGWCCY 3 cut(s) 449, 482, 924
PscI ACATGT 2 cut(s) 475, 1381
Psp5II RGGWCCY 3 cut(s) 449, 482, 924
Psp6I CCWGG 6 cut(s) 151, 267, 823, 919, 949, 1268
PspEI GGTNACC 2 cut(s) 860, 1264
PspFI CCCAGC 1 cut(s) 425
PspGI CCWGG 6 cut(s) 151, 267, 823, 919, 949, 1268
PspLI CGTACG 1 cut(s) 913
PspN4I GGNNCC 7 cut(s) 451, 483, 484, 550, 925, 948, 1627
PspPI GGNCC 9 cut(s) 449, 482, 549, 578, 754, 873, 924, 1049, 1625
PspPPI RGGWCCY 3 cut(s) 449, 482, 924
PsrI GAACNNNNNNTAC 4 cut(s) 522, 554, 1334, 1366
PstI CTGCAG 1 cut(s) 1090
PsuI RGATCY 1 cut(s) 1108
PvuI CGATCG 2 cut(s) 1081, 1473
RsaI GTAC 7 cut(s) 128, 511, 789, 915, 1021, 1124, 1499
RsaNI GTAC 7 cut(s) 127, 510, 788, 914, 1020, 1123, 1498
SalI GTCGAC 1 cut(s) 1538
SaqAI TTAA 4 cut(s) 438, 597, 1128, 1320
SatI GCNGC 3 cut(s) 742, 819, 1089
Sau3AI GATC 9 cut(s) 241, 245, 836, 1078, 1108, 1298, 1404, 1429, 1470
Sau96I GGNCC 9 cut(s) 449, 482, 549, 578, 754, 873, 924, 1049, 1625
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 8 cut(s) 153, 269, 278, 792, 825, 921, 951, 1270
SfaNI GCATC 2 cut(s) 90, 634
SfcI CTRYAG 1 cut(s) 1086
Sfr274I CTCGAG 1 cut(s) 1566
SinI GGWCC 6 cut(s) 449, 482, 549, 754, 873, 924
SlaI CTCGAG 1 cut(s) 1566
SmlI CTYRAG 1 cut(s) 1566
SmoI CTYRAG 1 cut(s) 1566
SsiI CCGC 4 cut(s) 284, 741, 757, 1040
SspI AATATT 2 cut(s) 347, 1654
SspMI CTAG 2 cut(s) 1325, 1535
StyD4I CCNGG 8 cut(s) 151, 267, 276, 790, 823, 919, 949, 1268
StyI CCWWGG 1 cut(s) 560
TaaI ACNGT 2 cut(s) 736, 1497
TaiI ACGT 4 cut(s) 789, 1104, 1143, 1335
TaqI TCGA 6 cut(s) 84, 244, 306, 1081, 1539, 1567
TatI WGTACW 2 cut(s) 1122, 1497
TauI GCSGC 1 cut(s) 744
TfiI GAWTC 2 cut(s) 1418, 1596
Tru1I TTAA 4 cut(s) 438, 597, 1128, 1320
Tru9I TTAA 4 cut(s) 438, 597, 1128, 1320
TscAI CASTG 3 cut(s) 467, 635, 1153
TseI GCWGC 2 cut(s) 818, 1088
TspDTI ATGAA 8 cut(s) 126, 179, 494, 674, 681, 896, 1478, 1656
TspGWI ACGGA 1 cut(s) 788
TspRI CASTG 3 cut(s) 467, 635, 1153
VpaK11BI GGWCC 6 cut(s) 449, 482, 549, 754, 873, 924
XapI RAATTY 2 cut(s) 1094, 1485
XceI RCATGY 3 cut(s) 229, 479, 1385
XhoI CTCGAG 1 cut(s) 1566
XmiI GTMKAC 1 cut(s) 1539
XspI CTAG 2 cut(s) 1325, 1535
Zsp2I ATGCAT 1 cut(s) 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.