MD15G1431500.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005319
Physical Location & Seq
Forward (+)
53128571 .. 53130334
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1431500.v1.1.491

Sequence Viewer

Length: 339 bp
ATGGTGGCGCCAAAGAAGACGAAGAAGACCCATGAGAGCATCAACACCAGGCTCGCTCTCGTCATGAAGAGCGGCAAATACACTCTTGGTTACAAAACCGTCATCGATTCCCTCCGCAGCTCCAAAGGCAAGTTGATCATTATCTCCAACAATTGCCCGCCTCTTCGGAAGTCGGAAATTGAATACTACGCCATGCTTGCGAAGATTGGGGTTCACCATTACAACGGAAACAATGTTGAGCTCGGTACAGCCTGCGGCAAGTATTTCCGTGTTTCCTGCCTTAGCATCATCGATGCAGGTGATTCGGATATCATAAAGACTCTACCTGGTGATCATTGA

Protein Analysis

113

Amino Acids

12.34

Weight (kDa)

9.52

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 13 - 105 9.4e-25 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 287
Acc36I ACCTGC 1 cut(s) 287
AccB1I GGYRCC 1 cut(s) 7
AccBSI CCGCTC 1 cut(s) 72
AciI CCGC 4 cut(s) 72, 115, 158, 255
AcyI GRCGYC 1 cut(s) 8
AfaI GTAC 1 cut(s) 247
AgsI TTSAA 1 cut(s) 182
AjnI CCWGG 2 cut(s) 47, 325
AluBI AGCT 2 cut(s) 120, 241
AluI AGCT 2 cut(s) 120, 241
Alw21I GWGCWC 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 117
AspLEI GCGC 1 cut(s) 10
AsuHPI GGTGA 2 cut(s) 206, 311
BanI GGYRCC 1 cut(s) 7
BanII GRGCYC 1 cut(s) 243
BbsI GAAGAC 2 cut(s) 23, 32
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 129
BcgI CGANNNNNNTGC 2 cut(s) 285, 319
BciT130I CCWGG 2 cut(s) 49, 327
BclI TGATCA 2 cut(s) 135, 331
BfoI RGCGCY 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 287
BisI GCNGC 3 cut(s) 73, 118, 256
BlsI GCNGC 3 cut(s) 74, 119, 257
Bme1390I CCNGG 2 cut(s) 49, 327
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 2 cut(s) 49, 327
BmsI GCATC 3 cut(s) 48, 283, 294
BpiI GAAGAC 2 cut(s) 23, 32
Bpu10I CCTNAGC 1 cut(s) 281
Bsa29I ATCGAT 2 cut(s) 105, 291
BsaBI GATNNNNATC 1 cut(s) 140
BsaHI GRCGYC 1 cut(s) 8
Bse8I GATNNNNATC 1 cut(s) 140
BseBI CCWGG 2 cut(s) 49, 327
BseCI ATCGAT 2 cut(s) 105, 291
BseJI GATNNNNATC 1 cut(s) 140
BseXI GCAGC 1 cut(s) 129
BshNI GGYRCC 1 cut(s) 7
BshVI ATCGAT 2 cut(s) 105, 291
BsiHKAI GWGCWC 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 2 cut(s) 135, 331
BspACI CCGC 4 cut(s) 72, 115, 158, 255
BspDI ATCGAT 2 cut(s) 105, 291
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 9
BspMI ACCTGC 1 cut(s) 287
BspQI GCTCTTC 1 cut(s) 62
BspT107I GGYRCC 1 cut(s) 7
BsrBI CCGCTC 1 cut(s) 72
BssMI GATC 2 cut(s) 135, 331
BssNI GRCGYC 1 cut(s) 8
Bst2UI CCWGG 2 cut(s) 49, 327
Bst4CI ACNGT 1 cut(s) 100
Bst6I CTCTTC 2 cut(s) 62, 168
BstACI GRCGYC 1 cut(s) 8
BstC8I GCNNGC 4 cut(s) 54, 158, 198, 253
BstDEI CTNAG 1 cut(s) 281
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 2 cut(s) 138, 334
BstMBI GATC 2 cut(s) 135, 331
BstMWI GCNNNNNNNGC 2 cut(s) 126, 197
BstNI CCWGG 2 cut(s) 49, 327
BstSCI CCNGG 2 cut(s) 47, 325
BstV1I GCAGC 1 cut(s) 129
BstV2I GAAGAC 2 cut(s) 23, 32
Bsu15I ATCGAT 2 cut(s) 105, 291
BsuTUI ATCGAT 2 cut(s) 105, 291
BveI ACCTGC 1 cut(s) 287
Cac8I GCNNGC 4 cut(s) 54, 158, 198, 253
CciI TCATGA 1 cut(s) 63
CfoI GCGC 1 cut(s) 10
ClaI ATCGAT 2 cut(s) 105, 291
CsiI ACCWGGT 1 cut(s) 325
Csp6I GTAC 1 cut(s) 246
CviAII CATG 3 cut(s) 32, 64, 193
CviJI RGCY 4 cut(s) 52, 120, 241, 251
CviKI_1 RGCY 4 cut(s) 52, 120, 241, 251
CviQI GTAC 1 cut(s) 246
DdeI CTNAG 1 cut(s) 281
DinI GGCGCC 1 cut(s) 9
DpnI GATC 2 cut(s) 137, 333
DpnII GATC 2 cut(s) 135, 331
Eam1104I CTCTTC 2 cut(s) 62, 168
EarI CTCTTC 2 cut(s) 62, 168
Ecl136II GAGCTC 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 243
Eco32I GATATC 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 241
EcoICRI GAGCTC 1 cut(s) 241
EcoRII CCWGG 2 cut(s) 47, 325
EcoRV GATATC 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 243
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
FaeI CATG 3 cut(s) 35, 67, 196
FaiI YATR 4 cut(s) 33, 65, 194, 314
FatI CATG 3 cut(s) 31, 63, 192
FauI CCCGC 1 cut(s) 165
FbaI TGATCA 2 cut(s) 135, 331
Fnu4HI GCNGC 3 cut(s) 73, 118, 256
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 3 cut(s) 73, 118, 256
GlaI GCGC 1 cut(s) 9
GluI GCNGC 3 cut(s) 73, 118, 256
HaeII RGCGCY 1 cut(s) 11
HhaI GCGC 1 cut(s) 10
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 3 cut(s) 35, 67, 196
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HinfI GANTC 3 cut(s) 107, 302, 319
HphI GGTGA 2 cut(s) 206, 311
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 3 cut(s) 168, 175, 307
Hpy188III TCNNGA 1 cut(s) 64
Hpy8I GTNNAC 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 100
HpyCH4V TGCA 1 cut(s) 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 197
HpyF3I CTNAG 1 cut(s) 281
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 3 cut(s) 35, 67, 196
HspAI GCGC 1 cut(s) 8
KasI GGCGCC 1 cut(s) 7
Ksp22I TGATCA 2 cut(s) 135, 331
Kzo9I GATC 2 cut(s) 135, 331
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 6 cut(s) 34, 61, 265, 282, 289, 312
Lsp1109I GCAGC 1 cut(s) 129
LweI GCATC 3 cut(s) 48, 283, 294
MabI ACCWGGT 1 cut(s) 325
MaeIII GTNAC 1 cut(s) 89
MalI GATC 2 cut(s) 137, 333
MbiI CCGCTC 1 cut(s) 72
MboI GATC 2 cut(s) 135, 331
MboII GAAGA 6 cut(s) 28, 34, 37, 79, 155, 214
MfeI CAATTG 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 2 cut(s) 151, 177
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 1 cut(s) 313
MmeI TCCRAC 2 cut(s) 153, 171
MnlI CCTC 2 cut(s) 122, 171
MspR9I CCNGG 2 cut(s) 49, 327
MunI CAATTG 1 cut(s) 151
MvaI CCWGG 2 cut(s) 49, 327
MwoI GCNNNNNNNGC 2 cut(s) 126, 197
NarI GGCGCC 1 cut(s) 8
NdeII GATC 2 cut(s) 135, 331
NlaIII CATG 3 cut(s) 35, 67, 196
NlaIV GGNNCC 1 cut(s) 9
PagI TCATGA 1 cut(s) 63
PaqCI CACCTGC 1 cut(s) 287
PciSI GCTCTTC 1 cut(s) 62
PfeI GAWTC 2 cut(s) 107, 302
PkrI GCNGC 3 cut(s) 74, 119, 257
PleI GAGTC 1 cut(s) 313
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 313
Psp124BI GAGCTC 1 cut(s) 243
Psp6I CCWGG 2 cut(s) 47, 325
PspGI CCWGG 2 cut(s) 47, 325
PspN4I GGNNCC 1 cut(s) 9
RsaI GTAC 1 cut(s) 247
RsaNI GTAC 1 cut(s) 246
SacI GAGCTC 1 cut(s) 243
SapI GCTCTTC 1 cut(s) 62
SatI GCNGC 3 cut(s) 73, 118, 256
Sau3AI GATC 2 cut(s) 135, 331
SchI GAGTC 1 cut(s) 313
ScrFI CCNGG 2 cut(s) 49, 327
SduI GDGCHC 1 cut(s) 243
SetI ASST 4 cut(s) 122, 243, 301, 328
SexAI ACCWGGT 1 cut(s) 325
SfaNI GCATC 3 cut(s) 48, 283, 294
SfoI GGCGCC 1 cut(s) 9
Sse9I AATT 2 cut(s) 151, 177
SsiI CCGC 4 cut(s) 72, 115, 158, 255
SspDI GGCGCC 1 cut(s) 7
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 2 cut(s) 47, 325
TaaI ACNGT 1 cut(s) 100
TaqI TCGA 2 cut(s) 105, 291
TasI AATT 2 cut(s) 151, 177
TauI GCSGC 2 cut(s) 75, 258
TfiI GAWTC 2 cut(s) 107, 302
TseI GCWGC 1 cut(s) 117
TspDTI ATGAA 1 cut(s) 80
TspGWI ACGGA 2 cut(s) 240, 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.