RLG00000000705

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005319
Physical Location & Seq
Reverse (-)
4092761 .. 4094135
1375 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000705

Sequence Viewer

Length: 339 bp
ATGGTGGCTGTGAAGAAAACCAAGAAGACCCATGAGTCTATCAACAACAGGCTCGCTCTTGTCATGAAGAGTGGCAAGTACACTCTGGGCTACAAGACCGTCATCGACACTCTTCGTAGCTCCAAAGGGAAGCTGATTATAATCTCGAACAATTGCCCTCCTCTGAGAAAGTCGGAGATCGAGTACTACGCCATGCTTGCTAAGGTTGGAGTTCATCACTACAATGGAAACAATGTTGACCTCGGTACAGCATGTGGCAAGTATTTCCGAGTGTCGTGCCTTAGCATTGTTGATCCAGGTGATTCGGATATCATCAAGACACTGCCTGGTGATCAGTGA

Protein Analysis

113

Amino Acids

12.35

Weight (kDa)

9.52

Isoelectric Point (pI)

34.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 13 - 105 7.4e-25 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 140
AasI GACNNNNNNGTC 1 cut(s) 34
AclWI GGATC 1 cut(s) 287
AfaI GTAC 3 cut(s) 80, 185, 247
AjnI CCWGG 2 cut(s) 295, 325
AluBI AGCT 2 cut(s) 120, 133
AluI AGCT 2 cut(s) 120, 133
AlwI GGATC 1 cut(s) 287
AsuHPI GGTGA 1 cut(s) 311
BbsI GAAGAC 1 cut(s) 32
BcgI CGANNNNNNTGC 2 cut(s) 258, 292
BciT130I CCWGG 2 cut(s) 297, 327
BclI TGATCA 1 cut(s) 331
BmcAI AGTACT 1 cut(s) 185
Bme1390I CCNGG 2 cut(s) 297, 327
BmrFI CCNGG 2 cut(s) 297, 327
BpiI GAAGAC 1 cut(s) 32
Bpu10I CCTNAGC 2 cut(s) 201, 281
BsaBI GATNNNNATC 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 241
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bse8I GATNNNNATC 1 cut(s) 140
BseBI CCWGG 2 cut(s) 297, 327
BseDI CCNNGG 1 cut(s) 241
BseJI GATNNNNATC 1 cut(s) 140
BseMII CTCAG 1 cut(s) 155
BseRI GAGGAG 1 cut(s) 150
Bsp143I GATC 3 cut(s) 177, 292, 331
BspCNI CTCAG 1 cut(s) 156
BspHI TCATGA 1 cut(s) 63
BspPI GGATC 1 cut(s) 287
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 3 cut(s) 177, 292, 331
Bst2UI CCWGG 2 cut(s) 297, 327
Bst4CI ACNGT 1 cut(s) 100
Bst6I CTCTTC 2 cut(s) 62, 117
BstC8I GCNNGC 2 cut(s) 54, 198
BstDEI CTNAG 3 cut(s) 164, 201, 281
BstKTI GATC 3 cut(s) 180, 295, 334
BstMBI GATC 3 cut(s) 177, 292, 331
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstNI CCWGG 2 cut(s) 297, 327
BstNSI RCATGY 1 cut(s) 255
BstSCI CCNGG 2 cut(s) 295, 325
BstV2I GAAGAC 1 cut(s) 32
BtsI GCAGTG 1 cut(s) 320
BtsIMutI CAGTG 1 cut(s) 320
Cac8I GCNNGC 2 cut(s) 54, 198
CciI TCATGA 1 cut(s) 63
Csp6I GTAC 3 cut(s) 79, 184, 246
CviAII CATG 4 cut(s) 32, 64, 193, 252
CviJI RGCY 5 cut(s) 8, 52, 90, 120, 133
CviKI_1 RGCY 5 cut(s) 8, 52, 90, 120, 133
CviQI GTAC 3 cut(s) 79, 184, 246
DdeI CTNAG 3 cut(s) 164, 201, 281
DpnI GATC 3 cut(s) 179, 294, 333
DpnII GATC 3 cut(s) 177, 292, 331
DrdI GACNNNNNNGTC 1 cut(s) 34
DseDI GACNNNNNNGTC 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 62, 117
EarI CTCTTC 2 cut(s) 62, 117
Eco32I GATATC 1 cut(s) 310
EcoRII CCWGG 2 cut(s) 295, 325
EcoRV GATATC 1 cut(s) 310
FaeI CATG 4 cut(s) 35, 67, 196, 255
FaiI YATR 5 cut(s) 33, 65, 140, 194, 253
FatI CATG 4 cut(s) 31, 63, 192, 251
FbaI TGATCA 1 cut(s) 331
Hin1II CATG 4 cut(s) 35, 67, 196, 255
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 2 cut(s) 35, 302
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 2 cut(s) 81, 238
Hpy188I TCNGA 4 cut(s) 165, 175, 269, 307
Hpy188III TCNNGA 3 cut(s) 64, 145, 316
Hpy8I GTNNAC 2 cut(s) 81, 238
HpyCH4III ACNGT 1 cut(s) 100
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 3 cut(s) 164, 201, 281
Hsp92II CATG 4 cut(s) 35, 67, 196, 255
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 3 cut(s) 177, 292, 331
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 5 cut(s) 34, 71, 282, 309, 312
MalI GATC 3 cut(s) 179, 294, 333
MboI GATC 3 cut(s) 177, 292, 331
MboII GAAGA 4 cut(s) 25, 37, 79, 104
MfeI CAATTG 1 cut(s) 151
MluCI AATT 1 cut(s) 151
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 2 cut(s) 153, 187
MnlI CCTC 3 cut(s) 168, 171, 251
MslI CAYNNNNRTG 1 cut(s) 222
MspR9I CCNGG 2 cut(s) 297, 327
MunI CAATTG 1 cut(s) 151
MvaI CCWGG 2 cut(s) 297, 327
MwoI GCNNNNNNNGC 1 cut(s) 197
NdeII GATC 3 cut(s) 177, 292, 331
NlaIII CATG 4 cut(s) 35, 67, 196, 255
NspI RCATGY 1 cut(s) 255
PagI TCATGA 1 cut(s) 63
PfeI GAWTC 1 cut(s) 302
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
PsiI TTATAA 1 cut(s) 140
Psp6I CCWGG 2 cut(s) 295, 325
PspGI CCWGG 2 cut(s) 295, 325
RsaI GTAC 3 cut(s) 80, 185, 247
RsaNI GTAC 3 cut(s) 79, 184, 246
RseI CAYNNNNRTG 1 cut(s) 222
Sau3AI GATC 3 cut(s) 177, 292, 331
ScaI AGTACT 1 cut(s) 185
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 2 cut(s) 297, 327
SetI ASST 5 cut(s) 122, 135, 207, 243, 301
SmiMI CAYNNNNRTG 1 cut(s) 222
Sse9I AATT 1 cut(s) 151
StyD4I CCNGG 2 cut(s) 295, 325
TaaI ACNGT 1 cut(s) 100
TaqI TCGA 3 cut(s) 105, 146, 180
TasI AATT 1 cut(s) 151
TatI WGTACW 2 cut(s) 78, 183
TfiI GAWTC 1 cut(s) 302
TscAI CASTG 1 cut(s) 327
TspDTI ATGAA 2 cut(s) 80, 203
TspRI CASTG 1 cut(s) 327
XceI RCATGY 1 cut(s) 255
ZrmI AGTACT 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.