MD16G1037200.v1.1

Cytidylyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004757
Physical Location & Seq
Forward (+)
2675464 .. 2678744
3281 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1037200.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGGATGACGACGATGGAGGAAGACAGGAGACGACGATGGAGGAAGACTGCATGAGTGCGCGCCCTAGTATGATGAGCGCGAGAACAAGGACACTAGTGGAAGCCATTCACTCGAGCCCCACGCAGTCCGTCCTCTATCTCGTCTGCGGAGCCTCTCAGGTCGTGGCGTGGCTGCTGTCGATTCCTGGAGCATCGAACACGGTCCTGGAAGCGGTGGTGCCCTACTCCAGAATGTCGATGATCCAATTGCTCGGCAAGATTCCGAACCAGTTTTGTAGTGCACACACTGCTGAAGAAATGGCTTTGTTGGCCTACAATCGCGCTCTCAAGCTCTCATCGCCAGGTTCCCCGGTTCTTGGTGTAGGTTTCACTGCTACTTTGGCTACCTCACGTCCAAAACTCGGAGACCACAGGCTTCACTTGTCAACAAGGACATCCGACAGACTTTGGGTGTCAACAGTGACTCTTTCTAAGGGTCTACGAAATCGAGAGGAAGAAGATATGGTTGCAAGTCATCTTTTACTCAAGGCAATTGCAAATTCATGCAAAGTTCCCGGGACATTTGTTTCAGAGCTCACTGAATCTGAAGTACCTGATGAATGTGAAATGAATTTTAGTGAAGATGAAGAGTTGGAGCAACTTATTAACGGGCAAATATGCTTTAAGGTGTATCCATTTTCAAGTGAACCACACATGTTGACTGCAGAAAGGAAGATAATATTGCCTGGTTCTTTTGATCCATTACATGAAGGTCACATGAAGCTCTTGGAGGCTGCTACTAGCTTATGCGGCAATGGCTATCCATGCTTTGAGATTTCAGCAGTCAATGCTGATAAACCTCCACTTTCAGTATCAGAAATCAAAGATCGTGTCAAGCAGTTTGAAAAAGTTGGAAAAACAGTCATTATATCCAATCAGCCATTTTTTTATAAGAAAGCTGAACTTTTCCCGGGCAGTGCTTTTGTGATTGGTGCGGACACAGCAGCGAGGCTGATTAATCCGAAGTACTATGACGGGGACTACAAGAAGATGCTGGAGATACTTCTTGGATGCAAGAAAACAGGGACCACTTTTCTTGTGGGTGGCCGTAATGTACATGGTGTTTTCAAGGTTCTTGAAGATTTTGATATTCCTGAGGAGTTAAGAGATATGTTCATCTCAATACCAGAGTTCCGCGTGGATTTATCATCCACCGAAATTAGGAAACAGGTTGGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTP_transf_like

Protein Analysis

407

Amino Acids

44.84

Weight (kDa)

5.51

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTP_transf_like PF01467 240 - 402 7.6e-09 Cytidylyltransferase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 930
AccB1I GGYRCC 1 cut(s) 217
AccI GTMKAC 1 cut(s) 478
AccII CGCG 4 cut(s) 61, 80, 321, 1176
AciI CCGC 5 cut(s) 147, 212, 789, 974, 1174
AclWI GGATC 2 cut(s) 235, 731
AcoI YGGCCR 1 cut(s) 1084
AcsI RAATTY 2 cut(s) 538, 610
AcuI CTGAAG 2 cut(s) 312, 606
AfaI GTAC 3 cut(s) 591, 1007, 1095
AfiI CCNNNNNNNGG 4 cut(s) 211, 356, 401, 1200
AflIII ACRYGT 1 cut(s) 693
AgsI TTSAA 4 cut(s) 681, 884, 1108, 1118
AhlI ACTAGT 1 cut(s) 94
AjiI CACGTC 1 cut(s) 392
AjnI CCWGG 4 cut(s) 184, 204, 340, 724
AluBI AGCT 5 cut(s) 331, 574, 763, 783, 938
AluI AGCT 5 cut(s) 331, 574, 763, 783, 938
Alw21I GWGCWC 2 cut(s) 283, 576
Alw26I GTCTC 2 cut(s) 23, 399
Alw44I GTGCAC 1 cut(s) 279
AlwI GGATC 2 cut(s) 235, 731
Ama87I CYCGRG 3 cut(s) 112, 554, 949
AoxI GGCC 2 cut(s) 309, 1084
ApaLI GTGCAC 1 cut(s) 279
ApeKI GCWGC 3 cut(s) 172, 773, 983
ApoI RAATTY 2 cut(s) 538, 610
ArsI GACNNNNNNTTYG 2 cut(s) 855, 887
AseI ATTAAT 1 cut(s) 996
Asp700I GAANNNNTTC 1 cut(s) 105
AspLEI GCGC 4 cut(s) 61, 63, 80, 323
AspS9I GGNCC 2 cut(s) 202, 1065
AsuC2I CCSGG 5 cut(s) 350, 555, 556, 950, 951
AvaI CYCGRG 3 cut(s) 112, 554, 949
AvaII GGWCC 2 cut(s) 202, 1065
AxyI CCTNAGG 1 cut(s) 1134
BaeGI GKGCMC 2 cut(s) 222, 283
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 2 cut(s) 119, 576
BbsI GAAGAC 2 cut(s) 28, 51
Bbv12I GWGCWC 2 cut(s) 283, 576
BbvI GCAGC 3 cut(s) 159, 760, 995
BccI CCATC 2 cut(s) 8, 31
BceAI ACGGC 1 cut(s) 1071
BciT130I CCWGG 4 cut(s) 186, 206, 342, 726
BciVI GTATCC 1 cut(s) 681
BcnI CCSGG 5 cut(s) 350, 555, 556, 950, 951
BcoDI GTCTC 2 cut(s) 23, 399
BcuI ACTAGT 1 cut(s) 94
BfaI CTAG 3 cut(s) 66, 95, 780
BfmI CTRYAG 1 cut(s) 702
BfuI GTATCC 1 cut(s) 681
BisI GCNGC 4 cut(s) 173, 774, 790, 984
BlsI GCNGC 4 cut(s) 174, 775, 791, 985
BmcAI AGTACT 1 cut(s) 1007
Bme1390I CCNGG 9 cut(s) 186, 206, 342, 350, 555, 556, 726, 950, 951
Bme18I GGWCC 2 cut(s) 202, 1065
BmeT110I CYCGRG 3 cut(s) 112, 554, 949
BmgBI CACGTC 1 cut(s) 392
BmgT120I GGNCC 2 cut(s) 202, 1065
BmiI GGNNCC 4 cut(s) 151, 219, 346, 1066
BmrFI CCNGG 9 cut(s) 186, 206, 342, 350, 555, 556, 726, 950, 951
BmsI GCATC 3 cut(s) 200, 1020, 1040
BpiI GAAGAC 2 cut(s) 28, 51
BpmI CTGGAG 3 cut(s) 207, 211, 1055
BpuEI CTTGAG 2 cut(s) 311, 509
BpuMI CCSGG 5 cut(s) 350, 555, 556, 950, 951
BsaI GGTCTC 1 cut(s) 399
BsaJI CCNNGG 3 cut(s) 348, 554, 949
Bsc4I CCNNNNNNNGG 4 cut(s) 211, 356, 401, 1200
Bse1I ACTGG 1 cut(s) 268
Bse21I CCTNAGG 1 cut(s) 1134
Bse3DI GCAATG 1 cut(s) 799
BseBI CCWGG 4 cut(s) 186, 206, 342, 726
BseDI CCNNGG 3 cut(s) 348, 554, 949
BseGI GGATG 4 cut(s) 10, 434, 1055, 1187
BseLI CCNNNNNNNGG 4 cut(s) 211, 356, 401, 1200
BseMI GCAATG 1 cut(s) 799
BseMII CTCAG 2 cut(s) 170, 1125
BseNI ACTGG 1 cut(s) 268
BsePI GCGCGC 1 cut(s) 59
BseRI GAGGAG 1 cut(s) 1151
BseSI GKGCMC 2 cut(s) 222, 283
BseXI GCAGC 3 cut(s) 159, 760, 995
Bsh1236I CGCG 4 cut(s) 61, 80, 321, 1176
BshFI GGCC 2 cut(s) 311, 1086
BshNI GGYRCC 1 cut(s) 217
BsiHKAI GWGCWC 2 cut(s) 283, 576
BsiHKCI CYCGRG 3 cut(s) 112, 554, 949
BsiSI CCGG 3 cut(s) 350, 555, 950
BslFI GGGAC 3 cut(s) 571, 1031, 1078
BslI CCNNNNNNNGG 4 cut(s) 211, 356, 401, 1200
BsmAI GTCTC 2 cut(s) 23, 399
BsmBI CGTCTC 1 cut(s) 23
BsmFI GGGAC 3 cut(s) 571, 1031, 1078
BsnI GGCC 2 cut(s) 311, 1086
Bso31I GGTCTC 1 cut(s) 399
BsoBI CYCGRG 3 cut(s) 112, 554, 949
Bsp1286I GDGCHC 4 cut(s) 119, 222, 283, 576
Bsp1407I TGTACA 1 cut(s) 1093
Bsp143I GATC 3 cut(s) 240, 736, 865
BspACI CCGC 5 cut(s) 147, 212, 789, 974, 1174
BspANI GGCC 2 cut(s) 311, 1086
BspCNI CTCAG 2 cut(s) 169, 1126
BspFNI CGCG 4 cut(s) 61, 80, 321, 1176
BspLI GGNNCC 4 cut(s) 151, 219, 346, 1066
BspMAI CTGCAG 1 cut(s) 706
BspPI GGATC 2 cut(s) 235, 731
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 399
BsrDI GCAATG 1 cut(s) 799
BsrGI TGTACA 1 cut(s) 1093
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 3 cut(s) 348, 554, 949
BssHII GCGCGC 1 cut(s) 59
BssMI GATC 3 cut(s) 240, 736, 865
Bst2UI CCWGG 4 cut(s) 186, 206, 342, 726
Bst4CI ACNGT 3 cut(s) 202, 460, 901
Bst6I CTCTTC 1 cut(s) 621
BstAPI GCANNNNNTGC 2 cut(s) 287, 827
BstAUI TGTACA 1 cut(s) 1093
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 3 cut(s) 156, 471, 1134
BstF5I GGATG 4 cut(s) 10, 434, 1055, 1187
BstFNI CGCG 4 cut(s) 61, 80, 321, 1176
BstHHI GCGC 4 cut(s) 61, 63, 80, 323
BstKTI GATC 3 cut(s) 243, 739, 868
BstMAI GTCTC 2 cut(s) 23, 399
BstMBI GATC 3 cut(s) 240, 736, 865
BstMWI GCNNNNNNNGC 9 cut(s) 287, 308, 337, 380, 789, 795, 804, 827, 980
BstNI CCWGG 4 cut(s) 186, 206, 342, 726
BstNSI RCATGY 1 cut(s) 697
BstSCI CCNGG 9 cut(s) 184, 204, 340, 348, 553, 554, 724, 948, 949
BstSFI CTRYAG 1 cut(s) 702
BstSLI GKGCMC 2 cut(s) 222, 283
BstUI CGCG 4 cut(s) 61, 80, 321, 1176
BstV1I GCAGC 3 cut(s) 159, 760, 995
BstV2I GAAGAC 2 cut(s) 28, 51
Bsu36I CCTNAGG 1 cut(s) 1134
BsuI GTATCC 1 cut(s) 681
BsuRI GGCC 2 cut(s) 311, 1086
BtgZI GCGATG 1 cut(s) 321
BtrI CACGTC 1 cut(s) 392
BtsCI GGATG 4 cut(s) 10, 434, 1055, 1187
BtsI GCAGTG 3 cut(s) 285, 369, 961
BtsIMutI CAGTG 5 cut(s) 285, 369, 465, 576, 961
Cac8I GCNNGC 1 cut(s) 61
CfoI GCGC 4 cut(s) 61, 63, 80, 323
Cfr13I GGNCC 2 cut(s) 202, 1065
Cfr9I CCCGGG 2 cut(s) 554, 949
Csp6I GTAC 3 cut(s) 590, 1006, 1094
CspCI CAANNNNNGTGG 4 cut(s) 678, 713, 1057, 1092
CviAII CATG 7 cut(s) 52, 543, 694, 746, 757, 804, 1097
CviQI GTAC 3 cut(s) 590, 1006, 1094
DdeI CTNAG 3 cut(s) 156, 471, 1134
DpnI GATC 3 cut(s) 242, 738, 867
DpnII GATC 3 cut(s) 240, 736, 865
EaeI YGGCCR 1 cut(s) 1084
Eam1104I CTCTTC 1 cut(s) 621
EarI CTCTTC 1 cut(s) 621
Ecl136II GAGCTC 1 cut(s) 574
Eco24I GRGCYC 2 cut(s) 119, 576
Eco31I GGTCTC 1 cut(s) 399
Eco47I GGWCC 2 cut(s) 202, 1065
Eco53kI GAGCTC 1 cut(s) 574
Eco57I CTGAAG 2 cut(s) 312, 606
Eco81I CCTNAGG 1 cut(s) 1134
Eco88I CYCGRG 3 cut(s) 112, 554, 949
EcoICRI GAGCTC 1 cut(s) 574
EcoRII CCWGG 4 cut(s) 184, 204, 340, 724
EcoT38I GRGCYC 2 cut(s) 119, 576
Esp3I CGTCTC 1 cut(s) 23
FaeI CATG 7 cut(s) 55, 546, 697, 749, 760, 807, 1100
FalI AAGNNNNNCTT 2 cut(s) 927, 959
FaqI GGGAC 3 cut(s) 571, 1031, 1078
FatI CATG 7 cut(s) 51, 542, 693, 745, 756, 803, 1096
FblI GTMKAC 1 cut(s) 478
Fnu4HI GCNGC 4 cut(s) 173, 774, 790, 984
FokI GGATG 4 cut(s) 17, 421, 1062, 1174
FriOI GRGCYC 2 cut(s) 119, 576
Fsp4HI GCNGC 4 cut(s) 173, 774, 790, 984
FspBI CTAG 3 cut(s) 66, 95, 780
GlaI GCGC 4 cut(s) 60, 62, 79, 322
GluI GCNGC 4 cut(s) 173, 774, 790, 984
GsuI CTGGAG 3 cut(s) 207, 211, 1055
HaeIII GGCC 2 cut(s) 311, 1086
HapII CCGG 3 cut(s) 350, 555, 950
HhaI GCGC 4 cut(s) 61, 63, 80, 323
Hin1II CATG 7 cut(s) 55, 546, 697, 749, 760, 807, 1100
Hin6I GCGC 4 cut(s) 59, 61, 78, 321
HinP1I GCGC 4 cut(s) 59, 61, 78, 321
HincII GTYRAC 3 cut(s) 426, 456, 699
HindII GTYRAC 3 cut(s) 426, 456, 699
HinfI GANTC 4 cut(s) 181, 259, 463, 581
HpaII CCGG 3 cut(s) 350, 555, 950
Hpy166II GTNNAC 6 cut(s) 281, 426, 456, 479, 686, 699
Hpy188I TCNGA 7 cut(s) 264, 404, 439, 571, 586, 856, 1002
Hpy188III TCNNGA 4 cut(s) 228, 488, 1115, 1133
Hpy8I GTNNAC 6 cut(s) 281, 426, 456, 479, 686, 699
Hpy99I CGWCG 2 cut(s) 14, 37
HpyAV CCTTC 1 cut(s) 743
HpyCH4III ACNGT 3 cut(s) 202, 460, 901
HpyCH4IV ACGT 1 cut(s) 391
HpyCH4V TGCA 7 cut(s) 51, 281, 509, 536, 546, 704, 1053
HpyF10VI GCNNNNNNNGC 9 cut(s) 287, 308, 337, 380, 789, 795, 804, 827, 980
HpyF3I CTNAG 3 cut(s) 156, 471, 1134
HpySE526I ACGT 1 cut(s) 391
Hsp92II CATG 7 cut(s) 55, 546, 697, 749, 760, 807, 1100
HspAI GCGC 4 cut(s) 59, 61, 78, 321
Kzo9I GATC 3 cut(s) 240, 736, 865
LmnI GCTCC 3 cut(s) 149, 188, 634
Lsp1109I GCAGC 3 cut(s) 159, 760, 995
LweI GCATC 3 cut(s) 200, 1020, 1040
MaeI CTAG 3 cut(s) 66, 95, 780
MaeII ACGT 1 cut(s) 391
MaeIII GTNAC 2 cut(s) 460, 752
MalI GATC 3 cut(s) 242, 738, 867
MboI GATC 3 cut(s) 240, 736, 865
MfeI CAATTG 2 cut(s) 245, 531
MhlI GDGCHC 4 cut(s) 119, 222, 283, 576
MluCI AATT 5 cut(s) 245, 531, 538, 610, 1197
MlyI GAGTC 1 cut(s) 457
MmeI TCCRAC 4 cut(s) 462, 612, 871, 1192
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 4 cut(s) 645, 663, 996, 1142
MspI CCGG 3 cut(s) 350, 555, 950
MspR9I CCNGG 9 cut(s) 186, 206, 342, 350, 555, 556, 726, 950, 951
MunI CAATTG 2 cut(s) 245, 531
MvaI CCWGG 4 cut(s) 186, 206, 342, 726
MvnI CGCG 4 cut(s) 61, 80, 321, 1176
MwoI GCNNNNNNNGC 9 cut(s) 287, 308, 337, 380, 789, 795, 804, 827, 980
NciI CCSGG 5 cut(s) 350, 555, 556, 950, 951
NdeII GATC 3 cut(s) 240, 736, 865
NlaIII CATG 7 cut(s) 55, 546, 697, 749, 760, 807, 1100
NlaIV GGNNCC 4 cut(s) 151, 219, 346, 1066
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 2 cut(s) 460, 752
NspI RCATGY 1 cut(s) 697
PaeR7I CTCGAG 1 cut(s) 112
PauI GCGCGC 1 cut(s) 59
PciI ACATGT 1 cut(s) 693
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 3 cut(s) 181, 259, 581
PfoI TCCNGGA 2 cut(s) 184, 204
PkrI GCNGC 4 cut(s) 174, 775, 791, 985
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PscI ACATGT 1 cut(s) 693
PshBI ATTAAT 1 cut(s) 996
PsiI TTATAA 1 cut(s) 930
Psp124BI GAGCTC 1 cut(s) 576
Psp6I CCWGG 4 cut(s) 184, 204, 340, 724
PspGI CCWGG 4 cut(s) 184, 204, 340, 724
PspN4I GGNNCC 4 cut(s) 151, 219, 346, 1066
PspPI GGNCC 2 cut(s) 202, 1065
PspXI VCTCGAGB 1 cut(s) 112
PstI CTGCAG 1 cut(s) 706
PteI GCGCGC 1 cut(s) 59
RsaI GTAC 3 cut(s) 591, 1007, 1095
RsaNI GTAC 3 cut(s) 590, 1006, 1094
SacI GAGCTC 1 cut(s) 576
SaqAI TTAA 4 cut(s) 645, 663, 996, 1142
SatI GCNGC 4 cut(s) 173, 774, 790, 984
Sau3AI GATC 3 cut(s) 240, 736, 865
Sau96I GGNCC 2 cut(s) 202, 1065
ScaI AGTACT 1 cut(s) 1007
SchI GAGTC 1 cut(s) 457
ScrFI CCNGG 9 cut(s) 186, 206, 342, 350, 555, 556, 726, 950, 951
SduI GDGCHC 4 cut(s) 119, 222, 283, 576
SfaNI GCATC 3 cut(s) 200, 1020, 1040
SfcI CTRYAG 1 cut(s) 702
Sfr274I CTCGAG 1 cut(s) 112
SinI GGWCC 2 cut(s) 202, 1065
SlaI CTCGAG 1 cut(s) 112
SmaI CCCGGG 2 cut(s) 556, 951
SmlI CTYRAG 3 cut(s) 112, 326, 524
SmoI CTYRAG 3 cut(s) 112, 326, 524
SpeI ACTAGT 1 cut(s) 94
Sse9I AATT 5 cut(s) 245, 531, 538, 610, 1197
SsiI CCGC 5 cut(s) 147, 212, 789, 974, 1174
SspI AATATT 1 cut(s) 720
SspMI CTAG 3 cut(s) 66, 95, 780
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 9 cut(s) 184, 204, 340, 348, 553, 554, 724, 948, 949
TaaI ACNGT 3 cut(s) 202, 460, 901
TaiI ACGT 1 cut(s) 394
TaqI TCGA 5 cut(s) 113, 179, 194, 236, 487
TasI AATT 5 cut(s) 245, 531, 538, 610, 1197
TatI WGTACW 2 cut(s) 1005, 1093
TauI GCSGC 1 cut(s) 792
TfiI GAWTC 3 cut(s) 181, 259, 581
Tru1I TTAA 4 cut(s) 645, 663, 996, 1142
Tru9I TTAA 4 cut(s) 645, 663, 996, 1142
TscAI CASTG 5 cut(s) 292, 376, 465, 583, 961
TseFI GTSAC 2 cut(s) 460, 752
TseI GCWGC 3 cut(s) 172, 773, 983
Tsp45I GTSAC 2 cut(s) 460, 752
TspDTI ATGAA 7 cut(s) 531, 612, 623, 639, 762, 773, 1144
TspGWI ACGGA 1 cut(s) 118
TspMI CCCGGG 2 cut(s) 554, 949
TspRI CASTG 5 cut(s) 292, 376, 465, 583, 961
VneI GTGCAC 1 cut(s) 279
VpaK11BI GGWCC 2 cut(s) 202, 1065
VspI ATTAAT 1 cut(s) 996
XapI RAATTY 2 cut(s) 538, 610
XceI RCATGY 1 cut(s) 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 1075
XhoI CTCGAG 1 cut(s) 112
XmaI CCCGGG 2 cut(s) 554, 949
XmiI GTMKAC 1 cut(s) 478
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 3 cut(s) 66, 95, 780
ZrmI AGTACT 1 cut(s) 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.