Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007383

Physical Location & Seq

Genomic Coordinates Reverse (-)

3705928 .. 3708357

Gene Structure 2430 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1051800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGGGAGATTTTTCGGGTTGTGATTTGATAAGCAATTTGCCTCAAAGCATAATAGAGAGCATTCTTACGCGTCTCCCGATCAGAGACGCTGTAAGAACTAGCTGCTTATCAAGGAAGTGGAGATACAAATGGACTACTATCACTCATCTTTCATTTGACGAAAAATGTGTCACTATATCAAATGACCGAGCTCTTGTTGAGAAAAGCCTCATAGATTTTGTTACTCGAGCTCTCTTTCTTCACCAAGGACCCATTCACAAGTTCCAGCTTTCAACTTCTTATTTGCAGAGCTGCCCGGATATTGATCAATGGATACTTTTCCTTTCGAGGAACGATATCAAAGAGTTGGTTCTTGAGTTGGGTGAAGGTGAGTGGTTTAGGGTGCCTTCGTGCCTTTTTTACTGTAAAAAGTTGACCCGTCTGGAGCTATTTCGCTGTGAGTTGGATCCGCCTCCTTCTTTTAAAGGATTTTTGTGTTTGAAGAGCCTCGATCTTCATCAGGTTTTGGTTACTCCTGATGCAATCGAAAGTCTTATTTCCAGTTGCCCTCTACTTGAGAGTCTGGCTTTGTCGTACTTCGATAGCTTAGCTCTAAACATCCGTGCTCCAAATCTCAGATATTTGTGTCTTGAAGGCGAATTTAAGGATATCTGTCTTGAAAATACTCCGCTTTTGGTTGCCATGTCTGTTGCTATGTATATGACTGACGGCATTGCTGAGCACTTTGAGCAAAGTTCAAATTGCAATTTTATCAAGTTTCTTGGTGGCATTCCACTTCTTGAGAGGCTTGTTGGGCATATCTACTTTACGAAGTATTTGAGTGTAGGTAATGACCTAGGAATTCTTCCGATTACTTACAATCGTTTAAAGATTATCGAATTATATCAAGTAAGTTTCGAAGATATGAATGAGATACTAGTTGTTCTTCGCTTGATTTCGAGCGCTCCCAACTTAAAGGAACTTCAAATCTCGGGATCTTCAAATGCTTTAGCAGCAATAGAAGCGTCCGACTTGGATTTCTGGGAGAAACAATGCCCTTCTGACTGCACATTCCGAAGGCTTAAAGTTGTGAAGATGACAGATATGTCTGGAGTGCCACATGAGATGGAATTCATCAAATTTTTGTTGAAGAAATCGCCCGTGCTTGAGATGATGAGCGTCATGCCTTGCGCGTATGGTTTGGAAGGACGGATGAACTTGTTGATTGAGTTGGTGAGGTTTAAACGGGCATCTCCTGAAGCAGAAATTATCTTCATCCAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box FBD LRR_2

Protein Analysis

422

Amino Acids

48.05

Weight (kDa)

5.46

Isoelectric Point (pI)

50.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	10 - 45	5.3e-08	F-box domain
LRR_At1g61320_AtMIF1	PF23622	88 - 196	2e-08	At1g61320/AtMIF1, LRR domain
LRR_At5g56370	PF24758	101 - 323	7.9e-18	FBD-associated F-box protein At5g56370, LRR repeats
FBD	PF08387	345 - 386	3.3e-07	FBD

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007383)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13570 AT1G13570 AT1G13570
fragaria_vesca	FvH4_4g28670 FvH4_4g28670 FvH4_4g28670 FvH4_4g28670
malus_domestica	MD13G1052800.v1.1 MD16G1051800.v1.1
prunus_persica	Prupe.1G299200_v2.0.a1 Prupe.1G299200_v2.0.a1 Prupe.1G299200_v2.0.a1
pyrus_communis	pycom13g04730 pycom16g04550
rosa_chinensis	RchiOBHm_Chr4g0436781
rosa_laevigata	RLG00000006496
rosa_multiflora	Rmu_co8098618.1_g000001 Rmu_sc0001837.1_g000005
rosa_roxburghii	Rroxscaffold_5G00377850
rosa_rugosa	Rorug04G0296000
rosa_samantha	Rh4AG350500 Rh4BG359400 Rh4CG373700 Rh4DG353400
rosa_wichuraiana	Rw4G030670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1084
AccB1I	GGYRCC	1 cut(s)	382
AccII	CGCG	2 cut(s)	70, 1172
AciI	CCGC	2 cut(s)	449, 668
AclWI	GGATC	3 cut(s)	440, 453, 982
AcsI	RAATTY	4 cut(s)	638, 840, 1109, 1118
AcuI	CTGAAG	1 cut(s)	1257
AfaI	GTAC	1 cut(s)	575
AfeI	AGCGCT	1 cut(s)	943
AflIII	ACRYGT	1 cut(s)	68
AgsI	TTSAA	8 cut(s)	273, 481, 632, 659, 738, 965, 981, 1129
AhlI	ACTAGT	1 cut(s)	916
AjuI	GAANNNNNNNTTGG	2 cut(s)	237, 269
AluBI	AGCT	8 cut(s)	102, 191, 230, 268, 291, 427, 585, 590
AluI	AGCT	8 cut(s)	102, 191, 230, 268, 291, 427, 585, 590
Alw21I	GWGCWC	4 cut(s)	193, 232, 607, 723
Alw26I	GTCTC	2 cut(s)	77, 78
AlwI	GGATC	3 cut(s)	440, 453, 982
Ama87I	CYCGRG	2 cut(s)	225, 970
Aor51HI	AGCGCT	1 cut(s)	943
ApeKI	GCWGC	3 cut(s)	102, 291, 992
ApoI	RAATTY	4 cut(s)	638, 840, 1109, 1118
ArsI	GACNNNNNNTTYG	2 cut(s)	153, 185
AspA2I	CCTAGG	1 cut(s)	835
AspLEI	GCGC	2 cut(s)	944, 1172
AspS9I	GGNCC	1 cut(s)	248
AsuC2I	CCSGG	1 cut(s)	296
AsuHPI	GGTGA	4 cut(s)	233, 374, 380, 1225
AsuII	TTCGAA	1 cut(s)	897
AvaI	CYCGRG	2 cut(s)	225, 970
AvaII	GGWCC	1 cut(s)	248
AvrII	CCTAGG	1 cut(s)	835
BamHI	GGATCC	1 cut(s)	445
BanI	GGYRCC	1 cut(s)	382
BanII	GRGCYC	2 cut(s)	193, 232
BarI	GAAGNNNNNNTAC	2 cut(s)	107, 139
Bbv12I	GWGCWC	4 cut(s)	193, 232, 607, 723
BbvI	GCAGC	3 cut(s)	89, 278, 1004
BccI	CCATC	1 cut(s)	1099
BceAI	ACGGC	1 cut(s)	724
BciVI	GTATCC	1 cut(s)	306
BclI	TGATCA	1 cut(s)	304
BcnI	CCSGG	1 cut(s)	296
BcoDI	GTCTC	2 cut(s)	77, 78
BcuI	ACTAGT	1 cut(s)	916
BfaI	CTAG	3 cut(s)	99, 836, 917
BfoI	RGCGCY	1 cut(s)	945
BfuI	GTATCC	1 cut(s)	306
BisI	GCNGC	3 cut(s)	103, 292, 993
BlnI	CCTAGG	1 cut(s)	835
BlpI	GCTNAGC	2 cut(s)	586, 717
BlsI	GCNGC	3 cut(s)	104, 293, 994
Bme1390I	CCNGG	1 cut(s)	296
Bme18I	GGWCC	1 cut(s)	248
BmeT110I	CYCGRG	2 cut(s)	225, 970
BmgT120I	GGNCC	1 cut(s)	248
BmiI	GGNNCC	3 cut(s)	250, 384, 447
BmrFI	CCNGG	1 cut(s)	296
BmsI	GCATC	2 cut(s)	508, 1238
BpmI	CTGGAG	2 cut(s)	443, 1110
Bpu1102I	GCTNAGC	2 cut(s)	586, 717
Bpu14I	TTCGAA	1 cut(s)	897
BpuEI	CTTGAG	4 cut(s)	374, 575, 800, 1166
BpuMI	CCSGG	1 cut(s)	296
BsaBI	GATNNNNATC	2 cut(s)	303, 495
BsaJI	CCNNGG	2 cut(s)	244, 835
Bse1I	ACTGG	1 cut(s)	540
Bse3DI	GCAATG	1 cut(s)	711
Bse8I	GATNNNNATC	2 cut(s)	303, 495
BseDI	CCNNGG	2 cut(s)	244, 835
BseGI	GGATG	3 cut(s)	597, 1197, 1254
BseJI	GATNNNNATC	2 cut(s)	303, 495
BseMI	GCAATG	1 cut(s)	711
BseMII	CTCAG	2 cut(s)	628, 708
BseNI	ACTGG	1 cut(s)	540
BseXI	GCAGC	3 cut(s)	89, 278, 1004
BsgI	GTGCAG	1 cut(s)	1030
Bsh1236I	CGCG	2 cut(s)	70, 1172
BshNI	GGYRCC	1 cut(s)	382
BsiHKAI	GWGCWC	4 cut(s)	193, 232, 607, 723
BsiHKCI	CYCGRG	2 cut(s)	225, 970
BsiSI	CCGG	1 cut(s)	296
BsmAI	GTCTC	2 cut(s)	77, 78
BsmBI	CGTCTC	2 cut(s)	77, 78
BsmI	GAATGC	2 cut(s)	60, 768
BsoBI	CYCGRG	2 cut(s)	225, 970
Bsp119I	TTCGAA	1 cut(s)	897
Bsp1286I	GDGCHC	4 cut(s)	193, 232, 607, 723
Bsp143I	GATC	5 cut(s)	78, 304, 445, 490, 974
Bsp1720I	GCTNAGC	2 cut(s)	586, 717
BspACI	CCGC	2 cut(s)	449, 668
BspCNI	CTCAG	2 cut(s)	627, 709
BspFNI	CGCG	2 cut(s)	70, 1172
BspLI	GGNNCC	3 cut(s)	250, 384, 447
BspPI	GGATC	3 cut(s)	440, 453, 982
BspQI	GCTCTTC	1 cut(s)	476
BspT104I	TTCGAA	1 cut(s)	897
BspT107I	GGYRCC	1 cut(s)	382
BsrDI	GCAATG	1 cut(s)	711
BsrI	ACTGG	1 cut(s)	540
BssECI	CCNNGG	2 cut(s)	244, 835
BssMI	GATC	5 cut(s)	78, 304, 445, 490, 974
BssT1I	CCWWGG	2 cut(s)	244, 835
Bst4CI	ACNGT	1 cut(s)	404
Bst6I	CTCTTC	1 cut(s)	476
BstBI	TTCGAA	1 cut(s)	897
BstDEI	CTNAG	3 cut(s)	586, 614, 717
BstF5I	GGATG	3 cut(s)	597, 1197, 1254
BstFNI	CGCG	2 cut(s)	70, 1172
BstH2I	RGCGCY	1 cut(s)	945
BstHHI	GCGC	2 cut(s)	944, 1172
BstKTI	GATC	5 cut(s)	81, 307, 448, 493, 977
BstMAI	GTCTC	2 cut(s)	77, 78
BstMBI	GATC	5 cut(s)	78, 304, 445, 490, 974
BstMWI	GCNNNNNNNGC	4 cut(s)	727, 793, 992, 1001
BstSCI	CCNGG	1 cut(s)	294
BstUI	CGCG	2 cut(s)	70, 1172
BstV1I	GCAGC	3 cut(s)	89, 278, 1004
BstX2I	RGATCY	2 cut(s)	445, 974
BstYI	RGATCY	2 cut(s)	445, 974
BsuI	GTATCC	1 cut(s)	306
BtsCI	GGATG	3 cut(s)	597, 1197, 1254
CfoI	GCGC	2 cut(s)	944, 1172
Cfr13I	GGNCC	1 cut(s)	248
CseI	GACGC	4 cut(s)	59, 95, 993, 1147
Csp6I	GTAC	1 cut(s)	574
CviAII	CATG	3 cut(s)	682, 1100, 1162
CviQI	GTAC	1 cut(s)	574
DdeI	CTNAG	3 cut(s)	586, 614, 717
DpnI	GATC	5 cut(s)	80, 306, 447, 492, 976
DpnII	GATC	5 cut(s)	78, 304, 445, 490, 974
DraI	TTTAAA	3 cut(s)	463, 867, 1222
DrdI	GACNNNNNNGTC	1 cut(s)	1084
DseDI	GACNNNNNNGTC	1 cut(s)	1084
Eam1104I	CTCTTC	1 cut(s)	476
EarI	CTCTTC	1 cut(s)	476
EciI	GGCGGA	1 cut(s)	438
Ecl136II	GAGCTC	2 cut(s)	191, 230
Eco130I	CCWWGG	2 cut(s)	244, 835
Eco24I	GRGCYC	2 cut(s)	193, 232
Eco32I	GATATC	2 cut(s)	337, 649
Eco47I	GGWCC	1 cut(s)	248
Eco47III	AGCGCT	1 cut(s)	943
Eco53kI	GAGCTC	2 cut(s)	191, 230
Eco57I	CTGAAG	1 cut(s)	1257
Eco88I	CYCGRG	2 cut(s)	225, 970
EcoICRI	GAGCTC	2 cut(s)	191, 230
EcoO109I	RGGNCCY	1 cut(s)	248
EcoRI	GAATTC	2 cut(s)	840, 1109
EcoRV	GATATC	2 cut(s)	337, 649
EcoT14I	CCWWGG	2 cut(s)	244, 835
EcoT38I	GRGCYC	2 cut(s)	193, 232
ErhI	CCWWGG	2 cut(s)	244, 835
Esp3I	CGTCTC	2 cut(s)	77, 78
FaeI	CATG	3 cut(s)	685, 1103, 1165
FatI	CATG	3 cut(s)	681, 1099, 1161
FbaI	TGATCA	1 cut(s)	304
Fnu4HI	GCNGC	3 cut(s)	103, 292, 993
FokI	GGATG	3 cut(s)	584, 1204, 1241
FriOI	GRGCYC	2 cut(s)	193, 232
Fsp4HI	GCNGC	3 cut(s)	103, 292, 993
FspBI	CTAG	3 cut(s)	99, 836, 917
GlaI	GCGC	2 cut(s)	943, 1171
GluI	GCNGC	3 cut(s)	103, 292, 993
GsuI	CTGGAG	2 cut(s)	443, 1110
HaeII	RGCGCY	1 cut(s)	945
HapII	CCGG	1 cut(s)	296
HgaI	GACGC	4 cut(s)	59, 95, 993, 1147
HhaI	GCGC	2 cut(s)	944, 1172
Hin1II	CATG	3 cut(s)	685, 1103, 1165
Hin6I	GCGC	2 cut(s)	942, 1170
HinP1I	GCGC	2 cut(s)	942, 1170
HincII	GTYRAC	1 cut(s)	414
HindII	GTYRAC	1 cut(s)	414
HinfI	GANTC	1 cut(s)	559
HpaII	CCGG	1 cut(s)	296
HphI	GGTGA	4 cut(s)	233, 374, 380, 1225
Hpy166II	GTNNAC	1 cut(s)	414
Hpy188I	TCNGA	6 cut(s)	83, 617, 849, 1009, 1042, 1055
Hpy8I	GTNNAC	1 cut(s)	414
HpyAV	CCTTC	7 cut(s)	359, 396, 465, 626, 1047, 1050, 1178
HpyCH4III	ACNGT	1 cut(s)	404
HpyCH4V	TGCA	4 cut(s)	286, 521, 744, 1047
HpyF10VI	GCNNNNNNNGC	4 cut(s)	727, 793, 992, 1001
HpyF3I	CTNAG	3 cut(s)	586, 614, 717
Hsp92II	CATG	3 cut(s)	685, 1103, 1165
HspAI	GCGC	2 cut(s)	942, 1170
Ksp22I	TGATCA	1 cut(s)	304
Kzo9I	GATC	5 cut(s)	78, 304, 445, 490, 974
LguI	GCTCTTC	1 cut(s)	476
LmnI	GCTCC	3 cut(s)	424, 610, 949
Lsp1109I	GCAGC	3 cut(s)	89, 278, 1004
LweI	GCATC	2 cut(s)	508, 1238
MaeI	CTAG	3 cut(s)	99, 836, 917
MaeIII	GTNAC	3 cut(s)	169, 220, 508
MalI	GATC	5 cut(s)	80, 306, 447, 492, 976
MboI	GATC	5 cut(s)	78, 304, 445, 490, 974
MflI	RGATCY	2 cut(s)	445, 974
MhlI	GDGCHC	4 cut(s)	193, 232, 607, 723
MluCI	AATT	9 cut(s)	34, 638, 739, 745, 840, 878, 1109, 1118, 1245
MluI	ACGCGT	1 cut(s)	68
MlyI	GAGTC	1 cut(s)	568
MmeI	TCCRAC	2 cut(s)	423, 1032
MnlI	CCTC	8 cut(s)	51, 218, 321, 462, 497, 558, 777, 1209
MseI	TTAA	6 cut(s)	462, 642, 866, 953, 1062, 1221
MspI	CCGG	1 cut(s)	296
MspR9I	CCNGG	1 cut(s)	296
MssI	GTTTAAAC	1 cut(s)	1222
Mva1269I	GAATGC	2 cut(s)	60, 768
MvnI	CGCG	2 cut(s)	70, 1172
MwoI	GCNNNNNNNGC	4 cut(s)	727, 793, 992, 1001
NciI	CCSGG	1 cut(s)	296
NdeII	GATC	5 cut(s)	78, 304, 445, 490, 974
NlaIII	CATG	3 cut(s)	685, 1103, 1165
NlaIV	GGNNCC	3 cut(s)	250, 384, 447
NmuCI	GTSAC	1 cut(s)	169
NspV	TTCGAA	1 cut(s)	897
PaeR7I	CTCGAG	1 cut(s)	225
PciSI	GCTCTTC	1 cut(s)	476
PcsI	WCGNNNNNNNCGW	1 cut(s)	74
PctI	GAATGC	2 cut(s)	60, 768
PkrI	GCNGC	3 cut(s)	104, 293, 994
PleI	GAGTC	1 cut(s)	567
PmeI	GTTTAAAC	1 cut(s)	1222
PpsI	GAGTC	1 cut(s)	567
PpuMI	RGGWCCY	1 cut(s)	248
Psp124BI	GAGCTC	2 cut(s)	193, 232
Psp5II	RGGWCCY	1 cut(s)	248
PspN4I	GGNNCC	3 cut(s)	250, 384, 447
PspPI	GGNCC	1 cut(s)	248
PspPPI	RGGWCCY	1 cut(s)	248
PspXI	VCTCGAGB	1 cut(s)	225
PsuI	RGATCY	2 cut(s)	445, 974
RsaI	GTAC	1 cut(s)	575
RsaNI	GTAC	1 cut(s)	574
SacI	GAGCTC	2 cut(s)	193, 232
SapI	GCTCTTC	1 cut(s)	476
SaqAI	TTAA	6 cut(s)	462, 642, 866, 953, 1062, 1221
SatI	GCNGC	3 cut(s)	103, 292, 993
Sau3AI	GATC	5 cut(s)	78, 304, 445, 490, 974
Sau96I	GGNCC	1 cut(s)	248
SchI	GAGTC	1 cut(s)	568
ScrFI	CCNGG	1 cut(s)	296
SduI	GDGCHC	4 cut(s)	193, 232, 607, 723
SfaNI	GCATC	2 cut(s)	508, 1238
Sfr274I	CTCGAG	1 cut(s)	225
SfuI	TTCGAA	1 cut(s)	897
SinI	GGWCC	1 cut(s)	248
SlaI	CTCGAG	1 cut(s)	225
SmlI	CTYRAG	5 cut(s)	225, 353, 554, 779, 1145
SmoI	CTYRAG	5 cut(s)	225, 353, 554, 779, 1145
SpeI	ACTAGT	1 cut(s)	916
Sse9I	AATT	9 cut(s)	34, 638, 739, 745, 840, 878, 1109, 1118, 1245
SsiI	CCGC	2 cut(s)	449, 668
SspMI	CTAG	3 cut(s)	99, 836, 917
SstI	GAGCTC	2 cut(s)	193, 232
StyD4I	CCNGG	1 cut(s)	294
StyI	CCWWGG	2 cut(s)	244, 835
TaaI	ACNGT	1 cut(s)	404
TaqI	TCGA	8 cut(s)	226, 326, 489, 525, 579, 876, 897, 938
TaqII	GACCGA	1 cut(s)	201
TasI	AATT	9 cut(s)	34, 638, 739, 745, 840, 878, 1109, 1118, 1245
Tru1I	TTAA	6 cut(s)	462, 642, 866, 953, 1062, 1221
Tru9I	TTAA	6 cut(s)	462, 642, 866, 953, 1062, 1221
TseFI	GTSAC	1 cut(s)	169
TseI	GCWGC	3 cut(s)	102, 291, 992
Tsp45I	GTSAC	1 cut(s)	169
TspDTI	ATGAA	6 cut(s)	141, 485, 920, 1102, 1208, 1243
TspGWI	ACGGA	2 cut(s)	590, 1204
VpaK11BI	GGWCC	1 cut(s)	248
XapI	RAATTY	4 cut(s)	638, 840, 1109, 1118
XhoI	CTCGAG	1 cut(s)	225
XmaJI	CCTAGG	1 cut(s)	835
XspI	CTAG	3 cut(s)	99, 836, 917

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.