Rh4DG353400

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007383
Physical Location & Seq
Reverse (-)
57481094 .. 57481881
788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG353400.1

Sequence Viewer

Length: 564 bp
ATGACTGATGAAAATGCTGAGAACTCTGAGCAAAGTTCAAATTGCAAGTTTATCAAGTTTCTTGGCGGCATACCACGCCTTGAGAGGCTTATTGGGCATATCTACTTTACTAAGTATTTGAGTATAGGTAAAGACCCGGGAGTCCTTCCAATTACATATAACCATCTAAAGATTATTGAATTATATCAAGTGAGTTTTGAAGATATGAAAGAGATACTTGTTGTTCTTCGCTTGATTACAAACTCTCCTAATTTAAAGGAACTTCAACTCTCGGGATCCTCTTTAACATCAATGGAATCACCGGATTTGGATTTCTGGGAGAAAGAAGGCCCTTCAGACTGCACATTCCACAGACTTAAAGTTGTGAAGATGACAGATATGTCCGGACTGCCACATGAGATGGAATTCATCAAATTTTTGTTGAGAAAATCACCGGTGCTTGAGATCATGAGTATTATGCCTTGTGTGTTTGTTACTATGGAGTCGCGTCTGATAATAGTCACGGAGTTGCTTAGTTTTACAAGGGCGTCTCCTAAAGCAGGAATTGTGTTCATCCAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

187

Amino Acids

21.35

Weight (kDa)

5.63

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBD PF08387 111 - 152 9.3e-08 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 140, 379
AccII CGCG 1 cut(s) 487
AccIII TCCGGA 1 cut(s) 383
AciI CCGC 1 cut(s) 66
AclWI GGATC 2 cut(s) 270, 283
AcsI RAATTY 2 cut(s) 404, 413
AcuI CTGAAG 1 cut(s) 318
AcyI GRCGYC 1 cut(s) 527
AfiI CCNNNNNNNGG 1 cut(s) 539
AgeI ACCGGT 1 cut(s) 433
AgsI TTSAA 4 cut(s) 39, 179, 200, 266
Alw26I GTCTC 1 cut(s) 534
AlwI GGATC 2 cut(s) 270, 283
Ama87I CYCGRG 2 cut(s) 136, 271
Aor13HI TCCGGA 1 cut(s) 383
AoxI GGCC 1 cut(s) 328
ApoI RAATTY 2 cut(s) 404, 413
AsiGI ACCGGT 1 cut(s) 433
AspS9I GGNCC 1 cut(s) 329
AsuC2I CCSGG 2 cut(s) 137, 138
AsuHPI GGTGA 2 cut(s) 291, 423
AvaI CYCGRG 2 cut(s) 136, 271
BamHI GGATCC 1 cut(s) 275
BccI CCATC 2 cut(s) 171, 394
BcnI CCSGG 2 cut(s) 137, 138
BcoDI GTCTC 1 cut(s) 534
BisI GCNGC 1 cut(s) 67
BlsI GCNGC 1 cut(s) 68
Bme1390I CCNGG 2 cut(s) 137, 138
BmeT110I CYCGRG 2 cut(s) 136, 271
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 2 cut(s) 137, 138
BpuEI CTTGAG 2 cut(s) 101, 461
BpuMI CCSGG 2 cut(s) 137, 138
BsaHI GRCGYC 1 cut(s) 527
BsaJI CCNNGG 1 cut(s) 136
BsaWI WCCGGW 3 cut(s) 301, 383, 433
BsaXI ACNNNNNCTCC 2 cut(s) 229, 259
Bsc4I CCNNNNNNNGG 1 cut(s) 539
Bse118I RCCGGY 1 cut(s) 433
BseAI TCCGGA 1 cut(s) 383
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 1 cut(s) 552
BseLI CCNNNNNNNGG 1 cut(s) 539
BseMII CTCAG 2 cut(s) 9, 18
BsgI GTGCAG 1 cut(s) 325
Bsh1236I CGCG 1 cut(s) 487
BshFI GGCC 1 cut(s) 330
BshTI ACCGGT 1 cut(s) 433
BsiHKCI CYCGRG 2 cut(s) 136, 271
BsiSI CCGG 4 cut(s) 137, 302, 384, 434
BslI CCNNNNNNNGG 1 cut(s) 539
BsmAI GTCTC 1 cut(s) 534
BsmBI CGTCTC 1 cut(s) 534
BsnI GGCC 1 cut(s) 330
BsoBI CYCGRG 2 cut(s) 136, 271
Bsp13I TCCGGA 1 cut(s) 383
Bsp143I GATC 2 cut(s) 275, 444
BspACI CCGC 1 cut(s) 66
BspANI GGCC 1 cut(s) 330
BspCNI CTCAG 2 cut(s) 10, 19
BspEI TCCGGA 1 cut(s) 383
BspFNI CGCG 1 cut(s) 487
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 1 cut(s) 277
BspPI GGATC 2 cut(s) 270, 283
BsrFI RCCGGY 1 cut(s) 433
BssAI RCCGGY 1 cut(s) 433
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 2 cut(s) 275, 444
BssNI GRCGYC 1 cut(s) 527
BstACI GRCGYC 1 cut(s) 527
BstDEI CTNAG 4 cut(s) 18, 27, 111, 512
BstENI CCTNNNNNAGG 1 cut(s) 537
BstF5I GGATG 1 cut(s) 552
BstFNI CGCG 1 cut(s) 487
BstKTI GATC 2 cut(s) 278, 447
BstMAI GTCTC 1 cut(s) 534
BstMBI GATC 2 cut(s) 275, 444
BstMWI GCNNNNNNNGC 2 cut(s) 75, 94
BstSCI CCNGG 2 cut(s) 135, 136
BstUI CGCG 1 cut(s) 487
BstX2I RGATCY 1 cut(s) 275
BstYI RGATCY 1 cut(s) 275
BsuRI GGCC 1 cut(s) 330
BtsCI GGATG 1 cut(s) 552
CciI TCATGA 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 433
Cfr13I GGNCC 1 cut(s) 329
Cfr9I CCCGGG 1 cut(s) 136
CseI GACGC 2 cut(s) 476, 516
CspAI ACCGGT 1 cut(s) 433
CviAII CATG 2 cut(s) 395, 448
CviJI RGCY 2 cut(s) 88, 330
CviKI_1 RGCY 2 cut(s) 88, 330
DdeI CTNAG 4 cut(s) 18, 27, 111, 512
DpnI GATC 2 cut(s) 277, 446
DpnII GATC 2 cut(s) 275, 444
DraI TTTAAA 1 cut(s) 255
DrdI GACNNNNNNGTC 2 cut(s) 140, 379
DseDI GACNNNNNNGTC 2 cut(s) 140, 379
Eco57I CTGAAG 1 cut(s) 318
Eco88I CYCGRG 2 cut(s) 136, 271
EcoNI CCTNNNNNAGG 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 404
Esp3I CGTCTC 1 cut(s) 534
FaeI CATG 2 cut(s) 398, 451
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FatI CATG 2 cut(s) 394, 447
Fnu4HI GCNGC 1 cut(s) 67
FokI GGATG 1 cut(s) 539
Fsp4HI GCNGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 67
HaeIII GGCC 1 cut(s) 330
HapII CCGG 4 cut(s) 137, 302, 384, 434
HgaI GACGC 2 cut(s) 476, 516
Hin1I GRCGYC 1 cut(s) 527
Hin1II CATG 2 cut(s) 398, 451
HinfI GANTC 3 cut(s) 141, 296, 482
HpaII CCGG 4 cut(s) 137, 302, 384, 434
HphI GGTGA 2 cut(s) 291, 423
Hpy188I TCNGA 3 cut(s) 28, 337, 492
Hpy188III TCNNGA 3 cut(s) 273, 384, 448
HpyAV CCTTC 3 cut(s) 155, 320, 342
HpyCH4V TGCA 2 cut(s) 45, 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 94
HpyF3I CTNAG 4 cut(s) 18, 27, 111, 512
Hsp92I GRCGYC 1 cut(s) 527
Hsp92II CATG 2 cut(s) 398, 451
Kpn2I TCCGGA 1 cut(s) 383
Kzo9I GATC 2 cut(s) 275, 444
LpnPI CCDG 6 cut(s) 150, 301, 315, 397, 447, 525
MaeIII GTNAC 2 cut(s) 472, 499
MalI GATC 2 cut(s) 277, 446
MboI GATC 2 cut(s) 275, 444
MboII GAAGA 3 cut(s) 212, 218, 379
MflI RGATCY 1 cut(s) 275
MluCI AATT 7 cut(s) 40, 150, 179, 250, 404, 413, 543
MlyI GAGTC 2 cut(s) 150, 491
MnlI CCTC 2 cut(s) 78, 289
MroI TCCGGA 1 cut(s) 383
MseI TTAA 3 cut(s) 254, 284, 357
MspI CCGG 4 cut(s) 137, 302, 384, 434
MspR9I CCNGG 2 cut(s) 137, 138
MvnI CGCG 1 cut(s) 487
MwoI GCNNNNNNNGC 2 cut(s) 75, 94
NciI CCSGG 2 cut(s) 137, 138
NdeII GATC 2 cut(s) 275, 444
NlaIII CATG 2 cut(s) 398, 451
NlaIV GGNNCC 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 499
PagI TCATGA 1 cut(s) 447
PfeI GAWTC 1 cut(s) 296
PinAI ACCGGT 1 cut(s) 433
PkrI GCNGC 1 cut(s) 68
PleI GAGTC 2 cut(s) 149, 490
PpsI GAGTC 2 cut(s) 149, 490
PspN4I GGNNCC 1 cut(s) 277
PspPI GGNCC 1 cut(s) 329
PsuI RGATCY 1 cut(s) 275
SaqAI TTAA 3 cut(s) 254, 284, 357
SatI GCNGC 1 cut(s) 67
Sau3AI GATC 2 cut(s) 275, 444
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 2 cut(s) 150, 491
ScrFI CCNGG 2 cut(s) 137, 138
SetI ASST 1 cut(s) 130
SgrAI CRCCGGYG 1 cut(s) 433
SmaI CCCGGG 1 cut(s) 138
SmlI CTYRAG 2 cut(s) 80, 440
SmoI CTYRAG 2 cut(s) 80, 440
Sse9I AATT 7 cut(s) 40, 150, 179, 250, 404, 413, 543
SsiI CCGC 1 cut(s) 66
StyD4I CCNGG 2 cut(s) 135, 136
TasI AATT 7 cut(s) 40, 150, 179, 250, 404, 413, 543
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 3 cut(s) 254, 284, 357
Tru9I TTAA 3 cut(s) 254, 284, 357
TseFI GTSAC 1 cut(s) 499
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 4 cut(s) 24, 221, 397, 541
TspGWI ACGGA 1 cut(s) 518
TspMI CCCGGG 1 cut(s) 136
XagI CCTNNNNNAGG 1 cut(s) 537
XapI RAATTY 2 cut(s) 404, 413
XmaI CCCGGG 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.