Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0007383

Physical Location & Seq

Genomic Coordinates Reverse (-)

3144296 .. 3146307

Gene Structure 2012 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom13g04730.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGAGGCGTGCTCGAAAGTCTAATCCTTCCCCACAGCCCACTGTGCTTTGTAGTCGATTGAGTTGGAATACAGAACAAATGGGCGATTTTTCGGGTTGTGATTCGATAAGCAATTTGCCTCAGGCCATTATAGAGAGCATTCTCACGCGTCTGCCAATAAGAGATGCTGTAAGAACTAGCTGTTTATCAACGAAATGGAGATACAAATGGACTACTATCACTCATCTTTCATTCGACGAAAAATGTGTCACTCTATCAAATGACCGAGCTCTTGTTGAAAAAAGCCTCATACGTTTTATTACTCGGGCACTCTTTCTTCACCAAGGACCCATTCACAAGTTCCAGCTTAAGACTTCATACTTGCAGAGCTGCCCGGATATAGATCGATGGATACTTTTTCTTTCAAGGAAAGATATCAAAGAACTGGTTCTTGAGTTGGGGGAAGGTGAGTGGTTTAGGGTGCCTTCGTGCCTTTTTTATTGTAAAAAATTGACCCGTTTGGAGCTATTTCGCTGCGAGTTGGATCCGCCTCCTGCTTTTAAAGGATTTTTGTGTTTGAAGGGCCTCAATCTTCATCAGGTTTTGGTTACTCCTGATGCAATTGAAAGTCTTATTTCCAGTTGCCCGCTACTTGAGAGTCTGGCTTTGTCATACTCTGATAGCTTAGCTCTAAACATCCGCGCTCCAAATCTCAGATACTTGTGTCTTGAAGGCGAATTTAAGGATATCTGTCTTGAAAATACGCCGCTTTTGGTTGCCATATCTGTTGCCATGTATATGACTGACGACATTGCTGAGCACTTTGAGCAAAGTTCAAATTGCAATTTTGTCAAGTTTCTTGGTGGCATTCCACTTCTTGAGAGGCTTGTTGGGCATATCTACTTTACAAAGTATTTGAGTATAGGTAATGACCTAGGAATTCTTCCAATTACTTACAACCGTTTAAAGATTATCGAATTATATCAAGTAAGTTTCGAAGATATGAAAGAGATACTAGTTGTTCTTCGCTTGATTACGAGCGCTCCCAACTTAAAGGAACTTCAAATCTCGGGATCTTCAAATACTTTAGCAGCAATAGAAGCGACCGACTTGGATTTCTGGGAGAAACAATGCCCTTCCGACTGCACATTCCGAAGGCTTAAAGTTGTGAAGATGACAGATATGTCCGGAGTGCCACACGAGATGGAATTCATGAAATTTCTATTGAAGAAATCACCCGTGCTTGAGACGATGAGCGTTACGCCTTGCGCGTATGTTTTGGAAGGACGGTTGAATATGTTGATTGAGTTGGCGAGGTTTAAACGGGCATCTCCGGAAGCAGAAGTTATCTTCATCCAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box FBD LRR_2

Protein Analysis

448

Amino Acids

51.08

Weight (kDa)

7.01

Isoelectric Point (pI)

49.98

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007383)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13570 AT1G13570 AT1G13570
fragaria_vesca	FvH4_4g28670 FvH4_4g28670 FvH4_4g28670 FvH4_4g28670
malus_domestica	MD13G1052800.v1.1 MD16G1051800.v1.1
prunus_persica	Prupe.1G299200_v2.0.a1 Prupe.1G299200_v2.0.a1 Prupe.1G299200_v2.0.a1
pyrus_communis	pycom13g04730 pycom16g04550
rosa_chinensis	RchiOBHm_Chr4g0436781
rosa_laevigata	RLG00000006496
rosa_multiflora	Rmu_co8098618.1_g000001 Rmu_sc0001837.1_g000005
rosa_roxburghii	Rroxscaffold_5G00377850
rosa_rugosa	Rorug04G0296000
rosa_samantha	Rh4AG350500 Rh4BG359400 Rh4CG373700 Rh4DG353400
rosa_wichuraiana	Rw4G030670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1162
AccB1I	GGYRCC	1 cut(s)	460
AccII	CGCG	3 cut(s)	148, 681, 1250
AccIII	TCCGGA	2 cut(s)	1166, 1312
AciI	CCGC	4 cut(s)	527, 626, 679, 746
AclWI	GGATC	3 cut(s)	518, 531, 1060
AcsI	RAATTY	4 cut(s)	716, 918, 1187, 1196
AfeI	AGCGCT	1 cut(s)	1021
AflII	CTTAAG	1 cut(s)	347
AflIII	ACRYGT	1 cut(s)	146
AhlI	ACTAGT	1 cut(s)	994
AjuI	GAANNNNNNNTTGG	2 cut(s)	315, 347
AluBI	AGCT	7 cut(s)	180, 269, 346, 369, 505, 663, 668
AluI	AGCT	7 cut(s)	180, 269, 346, 369, 505, 663, 668
Alw21I	GWGCWC	3 cut(s)	13, 271, 801
Alw26I	GTCTC	1 cut(s)	1220
AlwI	GGATC	3 cut(s)	518, 531, 1060
Ama87I	CYCGRG	2 cut(s)	303, 1048
Aor13HI	TCCGGA	2 cut(s)	1166, 1312
Aor51HI	AGCGCT	1 cut(s)	1021
AoxI	GGCC	2 cut(s)	123, 562
ApeKI	GCWGC	3 cut(s)	369, 513, 1070
ApoI	RAATTY	4 cut(s)	716, 918, 1187, 1196
ArsI	GACNNNNNNTTYG	2 cut(s)	231, 263
Asp700I	GAANNNNTTC	1 cut(s)	426
AspA2I	CCTAGG	1 cut(s)	913
AspLEI	GCGC	3 cut(s)	683, 1022, 1250
AspS9I	GGNCC	2 cut(s)	326, 562
AsuC2I	CCSGG	1 cut(s)	374
AsuHPI	GGTGA	3 cut(s)	311, 458, 1206
AsuII	TTCGAA	1 cut(s)	975
AvaI	CYCGRG	2 cut(s)	303, 1048
AvaII	GGWCC	1 cut(s)	326
AvrII	CCTAGG	1 cut(s)	913
AxyI	CCTNAGG	1 cut(s)	120
BaeGI	GKGCMC	1 cut(s)	310
BaeI	ACNNNNGTAYC	2 cut(s)	688, 721
BamHI	GGATCC	1 cut(s)	523
BanI	GGYRCC	1 cut(s)	460
BanII	GRGCYC	1 cut(s)	271
BauI	CACGAG	1 cut(s)	1178
Bbv12I	GWGCWC	3 cut(s)	13, 271, 801
BbvI	GCAGC	3 cut(s)	356, 500, 1082
BccI	CCATC	2 cut(s)	381, 1177
BciVI	GTATCC	1 cut(s)	384
BcnI	CCSGG	1 cut(s)	374
BcoDI	GTCTC	1 cut(s)	1220
BcuI	ACTAGT	1 cut(s)	994
BfaI	CTAG	3 cut(s)	177, 914, 995
BfoI	RGCGCY	1 cut(s)	1023
BfrI	CTTAAG	1 cut(s)	347
BfuI	GTATCC	1 cut(s)	384
BisI	GCNGC	4 cut(s)	370, 514, 746, 1071
BlnI	CCTAGG	1 cut(s)	913
BlpI	GCTNAGC	2 cut(s)	664, 795
BlsI	GCNGC	4 cut(s)	371, 515, 747, 1072
Bme1390I	CCNGG	1 cut(s)	374
Bme18I	GGWCC	1 cut(s)	326
BmeT110I	CYCGRG	2 cut(s)	303, 1048
BmgT120I	GGNCC	2 cut(s)	326, 562
BmiI	GGNNCC	3 cut(s)	328, 462, 525
BmrFI	CCNGG	1 cut(s)	374
BmsI	GCATC	3 cut(s)	154, 586, 1316
BplI	GAGNNNNNCTC	1 cut(s)	27
Bpu1102I	GCTNAGC	2 cut(s)	664, 795
Bpu14I	TTCGAA	1 cut(s)	975
BpuEI	CTTGAG	4 cut(s)	452, 653, 878, 1244
BpuMI	CCSGG	1 cut(s)	374
Bsa29I	ATCGAT	1 cut(s)	385
BsaBI	GATNNNNATC	1 cut(s)	381
BsaJI	CCNNGG	2 cut(s)	322, 913
BsaWI	WCCGGW	2 cut(s)	1166, 1312
BsaXI	ACNNNNNCTCC	2 cut(s)	1006, 1036
Bse1I	ACTGG	2 cut(s)	429, 618
Bse21I	CCTNAGG	1 cut(s)	120
Bse3DI	GCAATG	1 cut(s)	789
Bse8I	GATNNNNATC	1 cut(s)	381
BseAI	TCCGGA	2 cut(s)	1166, 1312
BseCI	ATCGAT	1 cut(s)	385
BseDI	CCNNGG	2 cut(s)	322, 913
BseGI	GGATG	2 cut(s)	675, 1332
BseJI	GATNNNNATC	1 cut(s)	381
BseMI	GCAATG	1 cut(s)	789
BseMII	CTCAG	3 cut(s)	134, 706, 786
BseNI	ACTGG	2 cut(s)	429, 618
BseSI	GKGCMC	1 cut(s)	310
BseXI	GCAGC	3 cut(s)	356, 500, 1082
BsgI	GTGCAG	1 cut(s)	1108
Bsh1236I	CGCG	3 cut(s)	148, 681, 1250
Bsh1285I	CGRYCG	1 cut(s)	1086
BshFI	GGCC	2 cut(s)	125, 564
BshNI	GGYRCC	1 cut(s)	460
BshVI	ATCGAT	1 cut(s)	385
BsiEI	CGRYCG	1 cut(s)	1086
BsiHKAI	GWGCWC	3 cut(s)	13, 271, 801
BsiHKCI	CYCGRG	2 cut(s)	303, 1048
BsiSI	CCGG	3 cut(s)	374, 1167, 1313
BsmAI	GTCTC	1 cut(s)	1220
BsmBI	CGTCTC	1 cut(s)	1220
BsmI	GAATGC	2 cut(s)	138, 846
BsnI	GGCC	2 cut(s)	125, 564
BsoBI	CYCGRG	2 cut(s)	303, 1048
Bsp119I	TTCGAA	1 cut(s)	975
Bsp1286I	GDGCHC	4 cut(s)	13, 271, 310, 801
Bsp13I	TCCGGA	2 cut(s)	1166, 1312
Bsp143I	GATC	3 cut(s)	382, 523, 1052
Bsp1720I	GCTNAGC	2 cut(s)	664, 795
BspACI	CCGC	4 cut(s)	527, 626, 679, 746
BspANI	GGCC	2 cut(s)	125, 564
BspCNI	CTCAG	3 cut(s)	133, 705, 787
BspDI	ATCGAT	1 cut(s)	385
BspEI	TCCGGA	2 cut(s)	1166, 1312
BspFNI	CGCG	3 cut(s)	148, 681, 1250
BspHI	TCATGA	1 cut(s)	1191
BspLI	GGNNCC	3 cut(s)	328, 462, 525
BspPI	GGATC	3 cut(s)	518, 531, 1060
BspT104I	TTCGAA	1 cut(s)	975
BspT107I	GGYRCC	1 cut(s)	460
BspTI	CTTAAG	1 cut(s)	347
BsrDI	GCAATG	1 cut(s)	789
BsrI	ACTGG	2 cut(s)	429, 618
BssECI	CCNNGG	2 cut(s)	322, 913
BssMI	GATC	3 cut(s)	382, 523, 1052
BssSI	CACGAG	1 cut(s)	1178
BssT1I	CCWWGG	2 cut(s)	322, 913
Bst2BI	CACGAG	1 cut(s)	1178
Bst4CI	ACNGT	3 cut(s)	43, 941, 1269
BstAFI	CTTAAG	1 cut(s)	347
BstBI	TTCGAA	1 cut(s)	975
BstC8I	GCNNGC	2 cut(s)	9, 626
BstDEI	CTNAG	4 cut(s)	120, 664, 692, 795
BstF5I	GGATG	2 cut(s)	675, 1332
BstFNI	CGCG	3 cut(s)	148, 681, 1250
BstH2I	RGCGCY	1 cut(s)	1023
BstHHI	GCGC	3 cut(s)	683, 1022, 1250
BstKTI	GATC	3 cut(s)	385, 526, 1055
BstMAI	GTCTC	1 cut(s)	1220
BstMBI	GATC	3 cut(s)	382, 523, 1052
BstMCI	CGRYCG	1 cut(s)	1086
BstMWI	GCNNNNNNNGC	4 cut(s)	43, 805, 871, 1079
BstSCI	CCNGG	1 cut(s)	372
BstSLI	GKGCMC	1 cut(s)	310
BstUI	CGCG	3 cut(s)	148, 681, 1250
BstV1I	GCAGC	3 cut(s)	356, 500, 1082
BstX2I	RGATCY	2 cut(s)	523, 1052
BstYI	RGATCY	2 cut(s)	523, 1052
Bsu15I	ATCGAT	1 cut(s)	385
Bsu36I	CCTNAGG	1 cut(s)	120
BsuI	GTATCC	1 cut(s)	384
BsuRI	GGCC	2 cut(s)	125, 564
BsuTUI	ATCGAT	1 cut(s)	385
BtsCI	GGATG	2 cut(s)	675, 1332
BtsIMutI	CAGTG	1 cut(s)	39
Cac8I	GCNNGC	2 cut(s)	9, 626
CciI	TCATGA	1 cut(s)	1191
CfoI	GCGC	3 cut(s)	683, 1022, 1250
Cfr13I	GGNCC	2 cut(s)	326, 562
ClaI	ATCGAT	1 cut(s)	385
CseI	GACGC	1 cut(s)	137
CviAII	CATG	2 cut(s)	772, 1192
DdeI	CTNAG	4 cut(s)	120, 664, 692, 795
DpnI	GATC	3 cut(s)	384, 525, 1054
DpnII	GATC	3 cut(s)	382, 523, 1052
DraI	TTTAAA	3 cut(s)	541, 945, 1300
DrdI	GACNNNNNNGTC	1 cut(s)	1162
DseDI	GACNNNNNNGTC	1 cut(s)	1162
EciI	GGCGGA	1 cut(s)	516
Ecl136II	GAGCTC	1 cut(s)	269
Eco130I	CCWWGG	2 cut(s)	322, 913
Eco24I	GRGCYC	1 cut(s)	271
Eco32I	GATATC	2 cut(s)	415, 727
Eco47I	GGWCC	1 cut(s)	326
Eco47III	AGCGCT	1 cut(s)	1021
Eco53kI	GAGCTC	1 cut(s)	269
Eco81I	CCTNAGG	1 cut(s)	120
Eco88I	CYCGRG	2 cut(s)	303, 1048
EcoICRI	GAGCTC	1 cut(s)	269
EcoO109I	RGGNCCY	2 cut(s)	326, 562
EcoRI	GAATTC	2 cut(s)	918, 1187
EcoRV	GATATC	2 cut(s)	415, 727
EcoT14I	CCWWGG	2 cut(s)	322, 913
EcoT38I	GRGCYC	1 cut(s)	271
ErhI	CCWWGG	2 cut(s)	322, 913
Esp3I	CGTCTC	1 cut(s)	1220
FaeI	CATG	2 cut(s)	775, 1195
FatI	CATG	2 cut(s)	771, 1191
FauI	CCCGC	1 cut(s)	633
Fnu4HI	GCNGC	4 cut(s)	370, 514, 746, 1071
FokI	GGATG	2 cut(s)	662, 1319
FriOI	GRGCYC	1 cut(s)	271
Fsp4HI	GCNGC	4 cut(s)	370, 514, 746, 1071
FspBI	CTAG	3 cut(s)	177, 914, 995
GlaI	GCGC	3 cut(s)	682, 1021, 1249
GluI	GCNGC	4 cut(s)	370, 514, 746, 1071
HaeII	RGCGCY	1 cut(s)	1023
HaeIII	GGCC	2 cut(s)	125, 564
HapII	CCGG	3 cut(s)	374, 1167, 1313
HgaI	GACGC	1 cut(s)	137
HhaI	GCGC	3 cut(s)	683, 1022, 1250
Hin1II	CATG	2 cut(s)	775, 1195
Hin6I	GCGC	3 cut(s)	681, 1020, 1248
HinP1I	GCGC	3 cut(s)	681, 1020, 1248
HinfI	GANTC	2 cut(s)	101, 637
HpaII	CCGG	3 cut(s)	374, 1167, 1313
HphI	GGTGA	3 cut(s)	311, 458, 1206
Hpy188I	TCNGA	4 cut(s)	658, 695, 1120, 1133
Hpy188III	TCNNGA	9 cut(s)	431, 593, 707, 734, 857, 1050, 1167, 1192, 1313
Hpy99I	CGWCG	1 cut(s)	239
HpyAV	CCTTC	8 cut(s)	36, 437, 474, 553, 704, 1125, 1128, 1256
HpyCH4III	ACNGT	3 cut(s)	43, 941, 1269
HpyCH4IV	ACGT	1 cut(s)	292
HpyCH4V	TGCA	4 cut(s)	364, 599, 822, 1125
HpyF10VI	GCNNNNNNNGC	4 cut(s)	43, 805, 871, 1079
HpyF3I	CTNAG	4 cut(s)	120, 664, 692, 795
HpySE526I	ACGT	1 cut(s)	292
Hsp92II	CATG	2 cut(s)	775, 1195
HspAI	GCGC	3 cut(s)	681, 1020, 1248
Kpn2I	TCCGGA	2 cut(s)	1166, 1312
Kzo9I	GATC	3 cut(s)	382, 523, 1052
LmnI	GCTCC	3 cut(s)	502, 688, 1027
Lsp1109I	GCAGC	3 cut(s)	356, 500, 1082
LweI	GCATC	3 cut(s)	154, 586, 1316
MaeI	CTAG	3 cut(s)	177, 914, 995
MaeII	ACGT	1 cut(s)	292
MaeIII	GTNAC	3 cut(s)	247, 586, 1237
MalI	GATC	3 cut(s)	384, 525, 1054
MboI	GATC	3 cut(s)	382, 523, 1052
MboII	GAAGA	9 cut(s)	308, 563, 914, 989, 995, 1047, 1162, 1219, 1321
MfeI	CAATTG	1 cut(s)	600
MflI	RGATCY	2 cut(s)	523, 1052
MhlI	GDGCHC	4 cut(s)	13, 271, 310, 801
MluI	ACGCGT	1 cut(s)	146
MlyI	GAGTC	1 cut(s)	646
MmeI	TCCRAC	3 cut(s)	44, 501, 1143
MnlI	CCTC	6 cut(s)	129, 296, 540, 575, 855, 1287
MroI	TCCGGA	2 cut(s)	1166, 1312
MroXI	GAANNNNTTC	1 cut(s)	426
MseI	TTAA	7 cut(s)	348, 540, 720, 944, 1031, 1140, 1299
MslI	CAYNNNNRTG	1 cut(s)	776
MspCI	CTTAAG	1 cut(s)	347
MspI	CCGG	3 cut(s)	374, 1167, 1313
MspR9I	CCNGG	1 cut(s)	374
MssI	GTTTAAAC	1 cut(s)	1300
MunI	CAATTG	1 cut(s)	600
Mva1269I	GAATGC	2 cut(s)	138, 846
MvnI	CGCG	3 cut(s)	148, 681, 1250
MwoI	GCNNNNNNNGC	4 cut(s)	43, 805, 871, 1079
NciI	CCSGG	1 cut(s)	374
NdeII	GATC	3 cut(s)	382, 523, 1052
NlaIII	CATG	2 cut(s)	775, 1195
NlaIV	GGNNCC	3 cut(s)	328, 462, 525
NmuCI	GTSAC	1 cut(s)	247
NspV	TTCGAA	1 cut(s)	975
PagI	TCATGA	1 cut(s)	1191
PcsI	WCGNNNNNNNCGW	1 cut(s)	1247
PctI	GAATGC	2 cut(s)	138, 846
PdmI	GAANNNNTTC	1 cut(s)	426
PfeI	GAWTC	1 cut(s)	101
PkrI	GCNGC	4 cut(s)	371, 515, 747, 1072
PleI	GAGTC	1 cut(s)	645
PmeI	GTTTAAAC	1 cut(s)	1300
PpsI	GAGTC	1 cut(s)	645
PpuMI	RGGWCCY	1 cut(s)	326
Psp124BI	GAGCTC	1 cut(s)	271
Psp5II	RGGWCCY	1 cut(s)	326
PspN4I	GGNNCC	3 cut(s)	328, 462, 525
PspPI	GGNCC	2 cut(s)	326, 562
PspPPI	RGGWCCY	1 cut(s)	326
PsuI	RGATCY	2 cut(s)	523, 1052
RseI	CAYNNNNRTG	1 cut(s)	776
SacI	GAGCTC	1 cut(s)	271
SaqAI	TTAA	7 cut(s)	348, 540, 720, 944, 1031, 1140, 1299
SatI	GCNGC	4 cut(s)	370, 514, 746, 1071
Sau3AI	GATC	3 cut(s)	382, 523, 1052
Sau96I	GGNCC	2 cut(s)	326, 562
SchI	GAGTC	1 cut(s)	646
ScrFI	CCNGG	1 cut(s)	374
SduI	GDGCHC	4 cut(s)	13, 271, 310, 801
SfaNI	GCATC	3 cut(s)	154, 586, 1316
SfuI	TTCGAA	1 cut(s)	975
SinI	GGWCC	1 cut(s)	326
SmiMI	CAYNNNNRTG	1 cut(s)	776
SmlI	CTYRAG	5 cut(s)	347, 431, 632, 857, 1223
SmoI	CTYRAG	5 cut(s)	347, 431, 632, 857, 1223
SpeI	ACTAGT	1 cut(s)	994
SsiI	CCGC	4 cut(s)	527, 626, 679, 746
SspMI	CTAG	3 cut(s)	177, 914, 995
SstI	GAGCTC	1 cut(s)	271
StyD4I	CCNGG	1 cut(s)	372
StyI	CCWWGG	2 cut(s)	322, 913
TaaI	ACNGT	3 cut(s)	43, 941, 1269
TaiI	ACGT	1 cut(s)	295
TaqI	TCGA	7 cut(s)	13, 55, 104, 234, 385, 954, 975
TaqII	GACCGA	2 cut(s)	279, 1100
TauI	GCSGC	1 cut(s)	748
TfiI	GAWTC	1 cut(s)	101
Tru1I	TTAA	7 cut(s)	348, 540, 720, 944, 1031, 1140, 1299
Tru9I	TTAA	7 cut(s)	348, 540, 720, 944, 1031, 1140, 1299
TscAI	CASTG	1 cut(s)	46
TseFI	GTSAC	1 cut(s)	247
TseI	GCWGC	3 cut(s)	369, 513, 1070
Tsp45I	GTSAC	1 cut(s)	247
TspDTI	ATGAA	7 cut(s)	219, 345, 563, 998, 1180, 1208, 1321
TspRI	CASTG	1 cut(s)	46
Vha464I	CTTAAG	1 cut(s)	347
VpaK11BI	GGWCC	1 cut(s)	326
XapI	RAATTY	4 cut(s)	716, 918, 1187, 1196
XmaJI	CCTAGG	1 cut(s)	913
XmnI	GAANNNNTTC	1 cut(s)	426
XspI	CTAG	3 cut(s)	177, 914, 995

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.