MD16G1088300.v1.1
ERF Family

superfamily. Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007183
Physical Location & Seq
Forward (+)
6088147 .. 6089667
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1088300.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGCCGCAACTGTTTCACTCTCGTTCTCTTTAGATCCGCAGCAGCAGCACCACCACCACCACTGCCATCTCTACCACTTCAGTCCCCGCCAAACTCTTCGCTTCCCTCCGTCAACCTCCTCATCTCTTAGTCTTCCCCCCAAATACCCACTTTCTTTTTTCCCCAATTCAGACCGTTTCTCCTTCAAGTCTTCCTCCTCCCCATCTCATTCTCCCACTACCACCACCGCTACTGATTCCACTAATACTGATTCCGCTGCTACTACTTCAACAGCTGCGTCATTCCTTGAATACCCACTTCGAACATGTAAGTTTCTGAGCAATGAAGAGCTCGAGCAGCTCAAACTCCTCGAGGGTTTCAGGTATTACCAGGAATTGGAATCTGGGTCGATGTGGGTTCGGGTGATGAGGCCTGAAGAGTTGGACATCACCGTTGGGTTGCTCGCCGAGTCGTTTGCGGAGTCGATGCTTTTGCCTTCTGCGTATGTGTCTGTGCTGGCATACTTGGTCAAGCAGTACTTGTTTGAGAGAATGGAGCTGGTTCCAAACACTGCCACGCTCATCGGGTTTTACAGAAGAAAAAAAGAAGAGGGAAAAGCAGAAGAAGAACAACTGGAAGAAGTAGAAGATGAAGTTGAGCTTTTGGCGGGGACTGTGGAAGTTTGCTTTAACAAAAAGGGGGCCAATGCTTCTCCTCCCACACCAACTCCTCCTAAGAATTCGCCTTATATAAGTAACATGGCCGTGAAAAAGTCGCTTCGGAGGAGGGGCATTGGCTGGCATCTTTTGAAGGCAAGCGAGGAACTAATCTCGCAGATGAGTTCCTCAAGAGAGGTGTACTTGCATTGCAGGATGATTGACACAGCTCCATTCAACATGTATAAAAAAGCAGGATACGACATTGTAAAGACGGATAACATTTTGGTTATGTTGCTGTTGCAGAGGCGCAAGCACTTAATGTGCAAGAAAATTCCAGTCTTAAACAGCTTTTCGGAATCAGATACGTCATGTTCTGAAGAGGAATCAACACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acetyltransf_1 Acetyltransf_10 FR47

Protein Analysis

345

Amino Acids

38.95

Weight (kDa)

6.28

Isoelectric Point (pI)

62.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 215 - 299 7.8e-07 Acetyltransferase (GNAT) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 350
AccB7I CCANNNNNTGG 1 cut(s) 376
AciI CCGC 7 cut(s) 6, 38, 88, 228, 255, 458, 647
AclWI GGATC 1 cut(s) 29
AcoI YGGCCR 2 cut(s) 3, 741
AcsI RAATTY 2 cut(s) 718, 969
AcuI CTGAAG 3 cut(s) 64, 435, 1035
AfaI GTAC 2 cut(s) 518, 839
AfiI CCNNNNNNNGG 1 cut(s) 376
AflIII ACRYGT 2 cut(s) 305, 876
AgsI TTSAA 5 cut(s) 187, 270, 290, 790, 874
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 7 cut(s) 275, 331, 340, 538, 640, 866, 987
AluI AGCT 7 cut(s) 275, 331, 340, 538, 640, 866, 987
Alw21I GWGCWC 1 cut(s) 333
AlwI GGATC 1 cut(s) 29
Ama87I CYCGRG 2 cut(s) 332, 350
AoxI GGCC 4 cut(s) 3, 410, 681, 741
ApeKI GCWGC 6 cut(s) 40, 43, 46, 257, 275, 337
ApoI RAATTY 2 cut(s) 718, 969
AspLEI GCGC 1 cut(s) 948
AspS9I GGNCC 1 cut(s) 681
AsuHPI GGTGA 2 cut(s) 415, 421
AsuII TTCGAA 1 cut(s) 301
AvaI CYCGRG 2 cut(s) 332, 350
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 2 cut(s) 125, 183
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 6 cut(s) 52, 55, 58, 244, 262, 349
BccI CCATC 2 cut(s) 75, 211
BceAI ACGGC 1 cut(s) 728
BcgI CGANNNNNNTGC 4 cut(s) 437, 454, 471, 488
BciT130I CCWGG 1 cut(s) 371
BciVI GTATCC 1 cut(s) 887
BfuI GTATCC 1 cut(s) 887
BisI GCNGC 7 cut(s) 6, 41, 44, 47, 258, 276, 338
BlsI GCNGC 7 cut(s) 7, 42, 45, 48, 259, 277, 339
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 1 cut(s) 371
BmeT110I CYCGRG 2 cut(s) 332, 350
BmgT120I GGNCC 1 cut(s) 681
BmiI GGNNCC 2 cut(s) 543, 682
BmrFI CCNGG 1 cut(s) 371
BmsI GCATC 2 cut(s) 456, 790
BpiI GAAGAC 2 cut(s) 125, 183
Bpu14I TTCGAA 1 cut(s) 301
BpuEI CTTGAG 1 cut(s) 811
BsaXI ACNNNNNCTCC 4 cut(s) 164, 194, 691, 721
Bsc4I CCNNNNNNNGG 1 cut(s) 376
Bse1I ACTGG 2 cut(s) 618, 974
Bse3DI GCAATG 2 cut(s) 328, 844
BseBI CCWGG 1 cut(s) 371
BseGI GGATG 1 cut(s) 858
BseLI CCNNNNNNNGG 1 cut(s) 376
BseMI GCAATG 2 cut(s) 328, 844
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 618, 974
BseRI GAGGAG 6 cut(s) 109, 187, 338, 684, 699, 778
BseXI GCAGC 6 cut(s) 52, 55, 58, 244, 262, 349
BshFI GGCC 4 cut(s) 5, 412, 683, 743
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 2 cut(s) 332, 350
BslFI GGGAC 2 cut(s) 69, 664
BslI CCNNNNNNNGG 1 cut(s) 376
BsmFI GGGAC 2 cut(s) 69, 664
BsnI GGCC 4 cut(s) 5, 412, 683, 743
BsoBI CYCGRG 2 cut(s) 332, 350
Bsp119I TTCGAA 1 cut(s) 301
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 1 cut(s) 34
BspACI CCGC 7 cut(s) 6, 38, 88, 228, 255, 458, 647
BspANI GGCC 4 cut(s) 5, 412, 683, 743
BspCNI CTCAG 1 cut(s) 309
BspLI GGNNCC 2 cut(s) 543, 682
BspPI GGATC 1 cut(s) 29
BspQI GCTCTTC 1 cut(s) 321
BspT104I TTCGAA 1 cut(s) 301
BsrDI GCAATG 2 cut(s) 328, 844
BsrI ACTGG 2 cut(s) 618, 974
BssMI GATC 1 cut(s) 34
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 4 cut(s) 13, 176, 433, 655
Bst6I CTCTTC 5 cut(s) 102, 321, 411, 582, 1011
BstBI TTCGAA 1 cut(s) 301
BstC8I GCNNGC 5 cut(s) 444, 498, 779, 796, 950
BstDEI CTNAG 3 cut(s) 128, 317, 714
BstF5I GGATG 1 cut(s) 858
BstHHI GCGC 1 cut(s) 948
BstKTI GATC 1 cut(s) 37
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 2 cut(s) 46, 337
BstNI CCWGG 1 cut(s) 371
BstNSI RCATGY 2 cut(s) 309, 880
BstSCI CCNGG 1 cut(s) 369
BstV1I GCAGC 6 cut(s) 52, 55, 58, 244, 262, 349
BstV2I GAAGAC 2 cut(s) 125, 183
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuI GTATCC 1 cut(s) 887
BsuRI GGCC 4 cut(s) 5, 412, 683, 743
BtsCI GGATG 1 cut(s) 858
BtsI GCAGTG 2 cut(s) 61, 549
BtsIMutI CAGTG 2 cut(s) 61, 549
Cac8I GCNNGC 5 cut(s) 444, 498, 779, 796, 950
CfoI GCGC 1 cut(s) 948
Cfr13I GGNCC 1 cut(s) 681
CseI GACGC 1 cut(s) 267
Csp6I GTAC 2 cut(s) 517, 838
CviAII CATG 5 cut(s) 306, 739, 877, 1008, 1032
CviQI GTAC 2 cut(s) 517, 838
DdeI CTNAG 3 cut(s) 128, 317, 714
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
EaeI YGGCCR 2 cut(s) 3, 741
Eam1104I CTCTTC 5 cut(s) 102, 321, 411, 582, 1011
EarI CTCTTC 5 cut(s) 102, 321, 411, 582, 1011
Ecl136II GAGCTC 1 cut(s) 331
Eco147I AGGCCT 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 333
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 3 cut(s) 64, 435, 1035
Eco88I CYCGRG 2 cut(s) 332, 350
EcoICRI GAGCTC 1 cut(s) 331
EcoRI GAATTC 1 cut(s) 718
EcoRII CCWGG 1 cut(s) 369
EcoT38I GRGCYC 1 cut(s) 333
FaeI CATG 5 cut(s) 309, 742, 880, 1011, 1035
FalI AAGNNNNNCTT 4 cut(s) 503, 535, 624, 656
FaqI GGGAC 2 cut(s) 69, 664
FatI CATG 5 cut(s) 305, 738, 876, 1007, 1031
FauI CCCGC 2 cut(s) 95, 640
Fnu4HI GCNGC 7 cut(s) 6, 41, 44, 47, 258, 276, 338
FokI GGATG 1 cut(s) 865
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 7 cut(s) 6, 41, 44, 47, 258, 276, 338
GlaI GCGC 1 cut(s) 947
GluI GCNGC 7 cut(s) 6, 41, 44, 47, 258, 276, 338
HaeIII GGCC 4 cut(s) 5, 412, 683, 743
HgaI GACGC 1 cut(s) 267
HhaI GCGC 1 cut(s) 948
Hin1II CATG 5 cut(s) 309, 742, 880, 1011, 1035
Hin6I GCGC 1 cut(s) 946
HinP1I GCGC 1 cut(s) 946
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 7 cut(s) 236, 251, 380, 449, 461, 995, 1022
HphI GGTGA 2 cut(s) 415, 421
Hpy166II GTNNAC 2 cut(s) 114, 838
Hpy188I TCNGA 6 cut(s) 172, 318, 762, 994, 1000, 1015
Hpy188III TCNNGA 1 cut(s) 828
Hpy8I GTNNAC 2 cut(s) 114, 838
HpyAV CCTTC 3 cut(s) 193, 486, 784
HpyCH4III ACNGT 4 cut(s) 13, 176, 433, 655
HpyCH4IV ACGT 1 cut(s) 1004
HpyCH4V TGCA 4 cut(s) 844, 849, 940, 963
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 337
HpyF3I CTNAG 3 cut(s) 128, 317, 714
HpySE526I ACGT 1 cut(s) 1004
Hsp92II CATG 5 cut(s) 309, 742, 880, 1011, 1035
HspAI GCGC 1 cut(s) 946
Kzo9I GATC 1 cut(s) 34
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 2 cut(s) 535, 871
Lsp1109I GCAGC 6 cut(s) 52, 55, 58, 244, 262, 349
LweI GCATC 2 cut(s) 456, 790
MaeII ACGT 1 cut(s) 1004
MaeIII GTNAC 1 cut(s) 734
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 4 cut(s) 166, 374, 718, 969
MlyI GAGTC 2 cut(s) 458, 470
MmeI TCCRAC 1 cut(s) 402
MseI TTAA 3 cut(s) 669, 956, 980
MslI CAYNNNNRTG 1 cut(s) 743
MspA1I CMGCKG 2 cut(s) 257, 275
MspR9I CCNGG 1 cut(s) 371
MvaI CCWGG 1 cut(s) 371
MwoI GCNNNNNNNGC 2 cut(s) 46, 337
NdeII GATC 1 cut(s) 34
NlaIII CATG 5 cut(s) 309, 742, 880, 1011, 1035
NlaIV GGNNCC 2 cut(s) 543, 682
NmeAIII GCCGAG 1 cut(s) 472
NspI RCATGY 2 cut(s) 309, 880
NspV TTCGAA 1 cut(s) 301
PaeR7I CTCGAG 2 cut(s) 332, 350
PceI AGGCCT 1 cut(s) 412
PciI ACATGT 2 cut(s) 305, 876
PciSI GCTCTTC 1 cut(s) 321
PfeI GAWTC 5 cut(s) 236, 251, 380, 995, 1022
PflMI CCANNNNNTGG 1 cut(s) 376
PkrI GCNGC 7 cut(s) 7, 42, 45, 48, 259, 277, 339
PleI GAGTC 2 cut(s) 457, 469
PpsI GAGTC 2 cut(s) 457, 469
PscI ACATGT 2 cut(s) 305, 876
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 2 cut(s) 543, 682
PspPI GGNCC 1 cut(s) 681
PspXI VCTCGAGB 2 cut(s) 332, 350
PsuI RGATCY 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 2 cut(s) 518, 839
RsaNI GTAC 2 cut(s) 517, 838
RseI CAYNNNNRTG 1 cut(s) 743
SacI GAGCTC 1 cut(s) 333
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 3 cut(s) 669, 956, 980
SatI GCNGC 7 cut(s) 6, 41, 44, 47, 258, 276, 338
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 1 cut(s) 681
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 2 cut(s) 458, 470
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 1 cut(s) 333
SfaNI GCATC 2 cut(s) 456, 790
Sfr274I CTCGAG 2 cut(s) 332, 350
SfuI TTCGAA 1 cut(s) 301
SlaI CTCGAG 2 cut(s) 332, 350
SmiMI CAYNNNNRTG 1 cut(s) 743
SmlI CTYRAG 3 cut(s) 332, 350, 826
SmoI CTYRAG 3 cut(s) 332, 350, 826
Sse9I AATT 4 cut(s) 166, 374, 718, 969
SseBI AGGCCT 1 cut(s) 412
SsiI CCGC 7 cut(s) 6, 38, 88, 228, 255, 458, 647
SstI GAGCTC 1 cut(s) 333
StuI AGGCCT 1 cut(s) 412
StyD4I CCNGG 1 cut(s) 369
TaaI ACNGT 4 cut(s) 13, 176, 433, 655
TaiI ACGT 1 cut(s) 1007
TaqI TCGA 5 cut(s) 301, 333, 351, 389, 464
TasI AATT 4 cut(s) 166, 374, 718, 969
TatI WGTACW 2 cut(s) 516, 837
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 5 cut(s) 236, 251, 380, 995, 1022
Tru1I TTAA 3 cut(s) 669, 956, 980
Tru9I TTAA 3 cut(s) 669, 956, 980
TscAI CASTG 2 cut(s) 68, 556
TseI GCWGC 6 cut(s) 40, 43, 46, 257, 275, 337
TspDTI ATGAA 2 cut(s) 339, 645
TspGWI ACGGA 2 cut(s) 99, 926
TspRI CASTG 2 cut(s) 68, 556
Van91I CCANNNNNTGG 1 cut(s) 376
XapI RAATTY 2 cut(s) 718, 969
XceI RCATGY 2 cut(s) 309, 880
XhoI CTCGAG 2 cut(s) 332, 350
ZrmI AGTACT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.